CN108849850A - A kind of human body cervical exfoliated cell preservative fluid - Google Patents
A kind of human body cervical exfoliated cell preservative fluid Download PDFInfo
- Publication number
- CN108849850A CN108849850A CN201710327971.0A CN201710327971A CN108849850A CN 108849850 A CN108849850 A CN 108849850A CN 201710327971 A CN201710327971 A CN 201710327971A CN 108849850 A CN108849850 A CN 108849850A
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- CN
- China
- Prior art keywords
- cell
- human body
- proclin300
- ethylene glycol
- cervical exfoliated
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0215—Disinfecting agents, e.g. antimicrobials for preserving living parts
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0221—Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention provides a kind of human body cervical exfoliated cell preservative fluids; it is made of sodium chloride, potassium chloride, proclin300, ethyl alcohol, ethylene glycol, sodium citrate, phosphate buffer, glutathione; present invention reduces use costs; really effectively protect the integrality and stationarity of cell; avoid cell dissolution and expansion; it easily promotes, compatibility is strong, saves the time for preparing sample.
Description
Technical field
The present invention relates to biologic product technology fields；More particularly to a kind of human body cervical exfoliated cell preservative fluid.
Background technique
Currently, normal human's living cells is with physiologic function, biological activity and complete cell shape before falling off
State has important value in biological study and medical test.If leaving human body, cell its physiology function for falling off
Energy, biological activity and cellular prion protein will also change.Therefore, how effectively to save cast-off cells form and structure is
Problem in need of consideration in cytology detection.Mainly from tradition and conventionally have following several inspection methods：
The smear made of traditional cytolgical examination method conventional smear (i.e. cervical smear), there are a large amount of cell overlaps
Phenomenon, and there are some heteroproteose cells without inspection value, this brings certain error to pathological examination.
And in conventional cervical carcinoma screening, there are the technical methods such as liquid based thinlayer cytology, PCR, bDNA.Liquid-based thin layer
Cytolgical examination (English abbreviation TCT) is one of common technological means, more next at present in American-European and China big and medium-sized cities
It is more widely used in cervical cytology screening.TCT is a kind of cervical smear technology of modernization, passes through acquisition
The cast-off cells of human body cervical surface with the form and structure of micro- these cells of sem observation after dyeing, and make diagnosis.
PCR and bDNA technology is to carry out the detection of DNA and RNA respectively using cervical epithelial cells.But from above-mentioned several method
It can be seen that being all to be detected using human body cervical exfoliated cell sample, thus cell-preservation liquid is that cell saves before detecting
It is crucial.Currently, the new Bai Shi in the U.S. (Thinprep cytologic test) cell-preservation liquid is and its instrument
(Thinpreper-2000) matching used special agent, but expensive, the treatment cost of making patients.
Therefore, the present invention is developed on the basis of mainly solving currently available technology and having above insufficient.
Summary of the invention
Present invention aim to address the above technical problem, especially set out a kind of to reduce cost, compatibility strong, effective right
The cell-preservation liquid of preservation is fixed in human body cervical exfoliated cell sample.
Based on above-mentioned purpose, technical problem solved by the invention is realized using following technical scheme：
A kind of human body cervical exfoliated cell preservative fluid, which is characterized in that it by sodium chloride, potassium chloride, proclin300, ethyl alcohol,
Ethylene glycol, sodium citrate, phosphate buffer, glutathione form, wherein sodium chloride 0.1-0.9%, potassium chloride 0.01-
0.09%, proclin300 0.01-0.1%, 10-50mM phosphate buffer（pH 7.0-7.4）, ethyl alcohol 10-30%, ethylene glycol
5-10%, sodium citrate 5-10mM, glutathione 1-10g/L.
Optimal, wherein sodium chloride 0.41%, potassium chloride 0.04%, proclin300 0.04%, 20mM phosphate-buffered
Liquid（pH 7.0）, ethyl alcohol 26%, ethylene glycol 6%, sodium citrate 6mM, glutathione 3g/L.
The technical principle of instant component:
Sodium chloride, potassium chloride：It is a kind of osmotic pressure maintenance agent.
Sodium citrate：It is a kind of resistant to aggregation reagent, is avoided that cell accumulation.
Glutathione：It is a kind of mucus inorganic agent, the tissue mucus in cell sample liquid can be decomposed to prevent mucus from rolling into a ball shadow
Ring the sedimentation film-making of cervical exfoliated cell.
proclin300：It is a kind of preservative, can effectively kills the pathogen such as bacterium, fungi, the saccharomycete in sample,
Extend the holding time for saving liquid, while working environment and staff's health can be protected.
Ethyl alcohol, ethylene glycol：Fixative quickly can have the cell examined and be worth to leucocyte, the epithelial cell etc. in sample
Preservation is fixed, it is intact effectively to save cellular morphology, while the integrality of intracellular nucleic acid can also be maintained.
Phosphate buffer：PH of buffer 7.0-7.4 can effectively maintain cell morphological characteristic.
The beneficial effects of the invention are as follows：
It 1, include that pH buffer, osmotic pressure maintain agent in the cell-preservation liquid, it is complete effectively to save cellular prion protein, nothing
Cell dissolution and cellular swelling phenomenon, the cell-preservation liquid contain cell fixative ingredient, can be effectively quickly to upper in sample
Preservation is fixed in the human bodies cervical exfoliated cells such as skin human body cervical exfoliated cell, leucocyte, prevents cell from self-dissolving occurs.
2, nucleolysis can be effectively reduced in the cell-preservation liquid, be easy to the detection research of subsequent nucleic acid.
3, compatibility is strong：It is raw that the cell of preservation can carry out the molecules such as immunocytochemistry, TCT, HPV-PCR, bDNA simultaneously
Object skill
Art detection, is not necessarily to multiple repairing weld.
4, ingredient is simple, cheap, easy to spread.
5, easy to operate using simple, the time for preparing sample is greatly saved.
Specific embodiment
Below by way of specific embodiment, the present invention is further explained.
A kind of human body cervical exfoliated cell preservative fluid, which is characterized in that it is by sodium chloride, potassium chloride, proclin300, second
Alcohol, ethylene glycol, sodium citrate, phosphate buffer, glutathione form, wherein sodium chloride 0.1-0.9%, potassium chloride 0.01-
0.09%, proclin300 0.01-0.1%, 10-50mM phosphate buffer（pH 7.0-7.4）, ethyl alcohol 10-30%, ethylene glycol
5-10%, sodium citrate 5-10mM, glutathione 1-10g/L.
Embodiment 1, cell-preservation liquid preparation method of the invention are sodium chloride 0.41%, potassium chloride 0.04%,
Proclin300 0.04%, 20mM phosphate buffer（pH 7.0）, ethyl alcohol 26%, ethylene glycol 6%, sodium citrate 6mM, paddy Guang
Sweet peptide 3g/L allows above-mentioned each component uniform stirring to dissolve.
The cell-preservation liquid made by above embodiments in use process, reduces use cost, really effective
The integrality and stationarity for protecting cell, avoid cell dissolution and expansion, easily promote, and compatibility is strong, save and prepare sample
Time.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit application field of the present invention.It is all in the present invention
Any modifications, equivalent replacements, and improvements etc. done within spirit and principle, should all be included in the protection scope of the present invention.
Claims (2)
1. a kind of human body cervical exfoliated cell preservative fluid, which is characterized in that it is by sodium chloride, potassium chloride, proclin300, second
Alcohol, ethylene glycol, sodium citrate, phosphate buffer, glutathione form, wherein sodium chloride 0.1-0.9%, potassium chloride 0.01-
0.09%, proclin300 0.01-0.1%, 10-50mM phosphate buffer（pH 7.0-7.4）, ethyl alcohol 10-30%, ethylene glycol
5-10%, sodium citrate 5-10mM, glutathione 1-10g/L.
2. the cell-preservation liquid according to claim 1, which is characterized in that sodium chloride 0.41%, potassium chloride 0.04%,
Proclin300 0.04%, 20mM phosphate buffer（pH 7.0）, ethyl alcohol 26%, ethylene glycol 6%, sodium citrate 6mM, paddy Guang
Sweet peptide 3g/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710327971.0A CN108849850A (en) | 2017-05-11 | 2017-05-11 | A kind of human body cervical exfoliated cell preservative fluid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710327971.0A CN108849850A (en) | 2017-05-11 | 2017-05-11 | A kind of human body cervical exfoliated cell preservative fluid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108849850A true CN108849850A (en) | 2018-11-23 |
Family
ID=64319368
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710327971.0A Pending CN108849850A (en) | 2017-05-11 | 2017-05-11 | A kind of human body cervical exfoliated cell preservative fluid |
Country Status (1)
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| CN (1) | CN108849850A (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109644985A (en) * | 2018-12-19 | 2019-04-19 | 易普森智慧健康科技（深圳）有限公司 | A kind of cell-preservation liquid and its application |
| CN111084179A (en) * | 2019-12-12 | 2020-05-01 | 郑州华之源医学检验实验室有限公司 | Cell preservation solution and preparation method thereof |
| CN111449056A (en) * | 2020-05-25 | 2020-07-28 | 山西汉济堂医疗科技有限公司 | Novel cell preservation solution and preparation method thereof |
| CN111972396A (en) * | 2019-05-21 | 2020-11-24 | 威海威高医用材料有限公司 | Cervical exfoliated cell preservation solution, preparation method and cell preservation method |
| CN113729009A (en) * | 2021-10-14 | 2021-12-03 | 山东高创医疗器械国家研究院有限公司 | Cervical cell preservation solution and preparation method and preservation method thereof |
| CN114052006A (en) * | 2021-11-16 | 2022-02-18 | 南京仁诺医学检验有限责任公司 | Cell preservation solution suitable for liquid-based cytology and nucleic acid detection |
| CN115735902A (en) * | 2022-11-16 | 2023-03-07 | 苏州颐坤生物科技有限公司 | Cell preservation solution and preparation method and application thereof |
-
2017
- 2017-05-11 CN CN201710327971.0A patent/CN108849850A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109644985A (en) * | 2018-12-19 | 2019-04-19 | 易普森智慧健康科技（深圳）有限公司 | A kind of cell-preservation liquid and its application |
| CN109644985B (en) * | 2018-12-19 | 2021-04-27 | 易普森智慧健康科技（深圳）有限公司 | Cell preservation solution and application thereof |
| CN111972396A (en) * | 2019-05-21 | 2020-11-24 | 威海威高医用材料有限公司 | Cervical exfoliated cell preservation solution, preparation method and cell preservation method |
| CN111084179A (en) * | 2019-12-12 | 2020-05-01 | 郑州华之源医学检验实验室有限公司 | Cell preservation solution and preparation method thereof |
| CN111449056A (en) * | 2020-05-25 | 2020-07-28 | 山西汉济堂医疗科技有限公司 | Novel cell preservation solution and preparation method thereof |
| CN113729009A (en) * | 2021-10-14 | 2021-12-03 | 山东高创医疗器械国家研究院有限公司 | Cervical cell preservation solution and preparation method and preservation method thereof |
| CN114052006A (en) * | 2021-11-16 | 2022-02-18 | 南京仁诺医学检验有限责任公司 | Cell preservation solution suitable for liquid-based cytology and nucleic acid detection |
| CN115735902A (en) * | 2022-11-16 | 2023-03-07 | 苏州颐坤生物科技有限公司 | Cell preservation solution and preparation method and application thereof |
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20181123 |
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| WD01 | Invention patent application deemed withdrawn after publication |