CN108849773B - 一种使家蚕日系茶斑限性品种的斑纹更加清晰的转育方法 - Google Patents
一种使家蚕日系茶斑限性品种的斑纹更加清晰的转育方法 Download PDFInfo
- Publication number
- CN108849773B CN108849773B CN201810661049.XA CN201810661049A CN108849773B CN 108849773 B CN108849773 B CN 108849773B CN 201810661049 A CN201810661049 A CN 201810661049A CN 108849773 B CN108849773 B CN 108849773B
- Authority
- CN
- China
- Prior art keywords
- tea
- spots
- individuals
- selfing
- female
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 241000255789 Bombyx mori Species 0.000 title claims abstract description 10
- 238000011426 transformation method Methods 0.000 title abstract description 4
- 241001122767 Theaceae Species 0.000 claims abstract description 47
- 238000000034 method Methods 0.000 claims abstract description 10
- 241000382353 Pupa Species 0.000 claims abstract description 7
- 238000009395 breeding Methods 0.000 claims abstract description 6
- 230000001488 breeding effect Effects 0.000 claims abstract description 5
- 230000000306 recurrent effect Effects 0.000 claims description 6
- 238000009396 hybridization Methods 0.000 claims description 3
- 230000002068 genetic effect Effects 0.000 abstract description 3
- 230000001186 cumulative effect Effects 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 description 15
- 108700003861 Dominant Genes Proteins 0.000 description 4
- 108700005079 Recessive Genes Proteins 0.000 description 3
- 102000052708 Recessive Genes Human genes 0.000 description 3
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 206010025421 Macule Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 238000010924 continuous production Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
- A01K67/04—Silkworms
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
本发明公开了一种使家蚕日系茶斑限性品种的斑纹更加清晰的转育方法,以筛选得到的茶斑限性品种为母本,与实用性日系品种杂交得到F1,选取雌与实用性日系品种回交得到BC1,在其后代中选留不包括深普茶的清晰茶斑个体自交得到F2,鉴蛹选留非深普茶的清晰茶斑雌与实用性日系品种连续回交二代,再采取相同步骤自交、回交,直至目的回交代数后自交,在选留的清晰淡普茶中鉴蛹获取雌与分开饲养的姬茶个体继续自交,开始累代单蛾育,单蛾育的第二代起整区淘汰出现的淡普斑或素蚕、不清晰茶斑蛾区,至雌个体中连续无非清晰茶斑出现。本发明成功利用了茶斑遗传新特点,采用特定材料与方法,使日系品种的茶斑更清晰,操作简单,过程快捷。
Description
采用本发明技术方案的一种使家蚕日系茶斑限性品种的斑纹更加清晰的转育方法,本发明的有益效果在于:基于通过连续回交育成法育成的所有日系茶斑限性品种因其限性茶斑偏浅而致使其实用化程度低的困难,开展遗传推理与分析,发现利用茶斑显性基因L与普通斑纹限性基因W⌒+构建的茶斑限性系统,其限性斑纹深浅程度还与其它基因(可能为多基因,此申报材料中把这个其它基因统一特指为第三方基因R,视为单基因)的作用有关。只是所参与试验的中系品种(包含茶斑显性基因材料)中有部分材料含有第三方基因R,而所参与试验的日系材料中则均不含有,其为隐性基因r。利用连续回交法,这部分含有第三方基因R的中系材料始终不会丢失第三方基因R,从始至终均表现为清晰的茶斑,而所有的日系材料连续回交过程中这第三方基因R均被隐性基因r所逐渐置换掉了,表现为茶斑斑纹相对较淡,不清晰,实用化程度低。利用筛选得到的具有第三方基因R的中系材料为亲本,通过杂交、回交、自交,再连续回交二次,再自交的轮回回交方法,各代继代个体在选留一同含有茶斑显性基因L、普通斑纹限性基因W^+、第二染色体上普通斑纹隐性基因p个体或群体的同时,也成功导入了与茶斑斑纹深浅表达有关的第三方基因R。打破了限性茶斑的表达只与茶斑显性基因L和限性普通斑纹基因W^+有关的固有认识。既加快育种进程,又提高了日系茶斑限性品种中限性茶斑显现度、清晰度与实用性。
附图说明
图1为本发明实施例的杂交导入步骤的流程示意图;
图2为本发明实施例的轮回回交步骤的流程示意图;
图3为本发明实施例的自交纯合步骤的流程示意图。
具体实施方式
如图1-3所示为本发明的优选实施例,其是以日系品种为普斑品种为例,如日系品种为素蚕品种或普斑限性品种,在轮回回交各代的继代个体选择中只需选取茶斑群体中清晰个体即可,自交纯合各代与本示意图所示的操作步骤与方法相一致。
本实施例的使家蚕日系茶斑限性品种的斑纹更加清晰的转育方法的具体步骤如下:
杂交导入:以筛选得到的茶斑限性品种ZW^+p/p L/LR/R(淡普茶,清晰,♀)为母本,实用性日系品种ZZ+/++L/+L r/r(普斑,♂)为父本,进行杂交,得到F1代;
轮回回交:鉴蛹选取茶斑雌个体ZW^++/p L/+L R/r与实用性日系品种雄个体ZZ+/++L/+L r/r回交得到BC1,选取清晰的普茶ZW^++/p L/+L R/r、ZZ+/+L/+L R/r或淡普茶ZZ+/pL/+L R/r自交得到F2代,回交父本如为普通斑纹品种ZZ+/++L/+Lr/r,则选取不包括深普茶个体在内的其它清晰地普茶或淡普茶个体,进一步鉴蛹选留雌个体与实用性日系品种雄个体ZZ+/++L/+L r/r连续回交二代,再采用相同方法与步骤自交、回交,直至达到目的回交代数;
自交纯合:自交得到Fn,在其后代中选留清晰的淡普茶个体,进一步鉴蛹选留雌个体与分开饲养的姬茶个体自交得到Fn+1,开始累代单蛾育,从Fn+2起整区淘汰凡是出现淡普斑或素蚕、以及不清晰茶斑的蛾区,直至雌个体中连续无非清晰茶斑出现为止。
附图1-3是一个连续的流程,图中:虚线框中表现型个体是根据继代遗传需要与斑纹区分难易来选留(姬茶个体的清晰与不清晰个体难以区分),从Fn代起姬茶个体应该分开饲养、分开上蔟。
对于本领域的技术人员来说,在不脱离本发明结构的前提下,还可以作出若干变形和改进,这些也应该视为本发明的保护范围,这些都不会影响本发明实施的效果和专利的实用性。
Claims (1)
1.一种使家蚕日系茶斑限性品种的斑纹更加清晰的转育方法,其特征在于:包括杂交导入、轮回回交和自交纯合,其操作步骤如下:
杂交导入:以筛选得到的优良茶斑限性品种为母本,实用性日系品种为父本,杂交得到F1;
轮回回交:鉴蛹选取茶斑雌个体与实用性日系品种雄个体回交得到BC1,在其后代中选取不包括深普茶的清晰茶斑个体自交得到F2,鉴蛹选留非深普斑的清晰茶斑雌个体与实用性日系品种雄个体连续回交二代,再采取相同步骤自交、回交,直至目的回交代数后连续自交;
自交纯合:在自交Fn代选留清晰的淡普茶个体中,进一步鉴蛹选留雌个体与分开饲养的姬茶个体自交得到Fn+1,开始累代单蛾育,从Fn+2起整区淘汰凡是出现淡普斑或素蚕、以及不清晰茶斑的蛾区,直至雌个体中连续无非清晰茶斑出现为止。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201810661049.XA CN108849773B (zh) | 2018-06-25 | 2018-06-25 | 一种使家蚕日系茶斑限性品种的斑纹更加清晰的转育方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201810661049.XA CN108849773B (zh) | 2018-06-25 | 2018-06-25 | 一种使家蚕日系茶斑限性品种的斑纹更加清晰的转育方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN108849773A CN108849773A (zh) | 2018-11-23 |
CN108849773B true CN108849773B (zh) | 2021-01-26 |
Family
ID=64294528
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201810661049.XA Active CN108849773B (zh) | 2018-06-25 | 2018-06-25 | 一种使家蚕日系茶斑限性品种的斑纹更加清晰的转育方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN108849773B (zh) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110367210A (zh) * | 2019-07-26 | 2019-10-25 | 四川省农业科学院蚕业研究所 | 家蚕限性斑纹深色化转育方法 |
CN113080153B (zh) * | 2021-05-07 | 2023-02-28 | 云南省农业科学院蚕桑蜜蜂研究所 | 一种将家蚕素斑品种定向转育成茶斑限性系统的雌回交法 |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
BG51182A1 (en) * | 1991-03-25 | 1993-03-15 | Darzhavna Sortova Komisija | SILKWORM HYBRID "154-K x C-3080" AND ITS REVERSE CROSS-BREED |
CN103190381A (zh) * | 2013-04-28 | 2013-07-10 | 贵州省蚕业研究所 | 双回交选育家蚕多元杂交种的育种方法 |
CN104430214A (zh) * | 2014-12-31 | 2015-03-25 | 广东省蚕业技术推广中心 | 一种强健性自出茧斑纹双限性家蚕品种的培育方法与应用 |
CN104886010A (zh) * | 2015-05-11 | 2015-09-09 | 艾均文 | 一种家蚕普斑品种的斑纹限性转育方法 |
CN105532588A (zh) * | 2015-12-05 | 2016-05-04 | 艾均文 | 一种利用家蚕普通斑纹品种构建实用性茶斑限性系统的简捷方法 |
-
2018
- 2018-06-25 CN CN201810661049.XA patent/CN108849773B/zh active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
BG51182A1 (en) * | 1991-03-25 | 1993-03-15 | Darzhavna Sortova Komisija | SILKWORM HYBRID "154-K x C-3080" AND ITS REVERSE CROSS-BREED |
CN103190381A (zh) * | 2013-04-28 | 2013-07-10 | 贵州省蚕业研究所 | 双回交选育家蚕多元杂交种的育种方法 |
CN104430214A (zh) * | 2014-12-31 | 2015-03-25 | 广东省蚕业技术推广中心 | 一种强健性自出茧斑纹双限性家蚕品种的培育方法与应用 |
CN104886010A (zh) * | 2015-05-11 | 2015-09-09 | 艾均文 | 一种家蚕普斑品种的斑纹限性转育方法 |
CN105532588A (zh) * | 2015-12-05 | 2016-05-04 | 艾均文 | 一种利用家蚕普通斑纹品种构建实用性茶斑限性系统的简捷方法 |
Non-Patent Citations (2)
Title |
---|
家蚕限性茶斑系的选育;朱勇 等;《蚕学通讯》;19921231(第4期);第14-16页 * |
日欧系形蚕品种转育成限性斑纹品种的研究;承经宇 等;《陕西蚕业》;19921231(第1期);第5-7、14页 * |
Also Published As
Publication number | Publication date |
---|---|
CN108849773A (zh) | 2018-11-23 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
De Barro et al. | Spatial and temporal genetic variation in British field populations of the grain aphid Sitobion avenae (F.)(Hemiptera: Aphididae) studied using RAPD-PCR | |
CN108849773B (zh) | 一种使家蚕日系茶斑限性品种的斑纹更加清晰的转育方法 | |
Matsushita et al. | Allopolyploidization lays the foundation for evolution of distinct populations: evidence from analysis of synthetic Arabidopsis allohexaploids | |
Smithson et al. | The evolution of empty flowers revisited | |
Gaebelein et al. | Allohexaploids in the genus Brassica | |
Callac et al. | A novel homothallic variety of Agaricus bisporus comprises rare tetrasporic isolates from Europe | |
CN104886010B (zh) | 一种家蚕普斑品种的斑纹限性转育方法 | |
CN105532588A (zh) | 一种利用家蚕普通斑纹品种构建实用性茶斑限性系统的简捷方法 | |
Crawford et al. | Genetic diversity in Asteraceae endemic to oceanic islands: Baker’s Law and polyploidy | |
Schwenk et al. | Experimental interspecific hybridization in Daphnia | |
Kopecky et al. | Reduction of ploidy level by androgenesis in intergeneric Lolium–Festuca hybrids for turf grass breeding | |
US20190246589A1 (en) | New Guinea Impatiens Variety SAKIMP051 | |
US20170202165A1 (en) | New Guinea Impatiens Variety SAKIMP040 | |
US9826690B2 (en) | Cucurbita pepo hybrids | |
US10420317B2 (en) | Petunia variety KLEPH18389 | |
US10258005B1 (en) | New Guinea impatiens variety SAKIMP045 | |
Denton et al. | Genome-specific histories of divergence and introgression between an allopolyploid unisexual salamander lineage and two ancestral sexual species | |
US10271510B2 (en) | Petunia variety KLEPH17342 | |
US10149452B2 (en) | Guinea Impatiens variety SAKIMP044 | |
US11707046B2 (en) | Petunia variety ‘KLEPH20580’ | |
US10925251B1 (en) | Petunia variety KLEPH18370 | |
US20220217935A1 (en) | Petunia Variety KLEPH20513 | |
US10463002B2 (en) | New Guinea Impatiens variety SAKIMP047 | |
US10721896B2 (en) | Year round flowering lupine | |
US11252928B2 (en) | Miniature rose plant named ‘Meibenbino’ |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right |
Effective date of registration: 20210419 Address after: 410000 Hunan province Changsha Furong district in the Branch Road No. 23 Patentee after: Hunan Institute of Sericulture Patentee after: SOUTHWEST University Address before: 410000 Hunan province Changsha Furong district in the Branch Road No. 23 Patentee before: Hunan Institute of Sericulture |
|
TR01 | Transfer of patent right |