CN108844897A - A kind of sampling device structure and optical projection tomography system - Google Patents
A kind of sampling device structure and optical projection tomography system Download PDFInfo
- Publication number
- CN108844897A CN108844897A CN201810870777.1A CN201810870777A CN108844897A CN 108844897 A CN108844897 A CN 108844897A CN 201810870777 A CN201810870777 A CN 201810870777A CN 108844897 A CN108844897 A CN 108844897A
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- test tube
- horizontal panel
- sampling device
- link block
- mounting base
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- 238000005070 sampling Methods 0.000 title claims abstract description 23
- 230000003287 optical effect Effects 0.000 title claims abstract description 11
- 238000003325 tomography Methods 0.000 title claims abstract description 11
- 230000007246 mechanism Effects 0.000 claims description 30
- 239000005338 frosted glass Substances 0.000 claims description 9
- 230000001105 regulatory effect Effects 0.000 claims description 9
- 230000005389 magnetism Effects 0.000 claims description 7
- 230000001276 controlling effect Effects 0.000 claims description 6
- 230000008450 motivation Effects 0.000 claims description 2
- -1 poly- Perfluoroethylene-propylene Chemical group 0.000 claims description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 2
- 229920001577 copolymer Polymers 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 8
- 230000008569 process Effects 0.000 abstract description 6
- 239000000523 sample Substances 0.000 description 21
- 238000003384 imaging method Methods 0.000 description 11
- 230000000694 effects Effects 0.000 description 8
- 230000006872 improvement Effects 0.000 description 6
- 238000010586 diagram Methods 0.000 description 5
- 239000012472 biological sample Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000696 magnetic material Substances 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 241000219194 Arabidopsis Species 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Analysing Materials By The Use Of Radiation (AREA)
Abstract
The invention discloses a kind of sampling device structure and optical projection tomography systems, the sampling device includes mounting base, loading component, test tube and fixation kit, the mounting base includes horizontal panel, the horizontal panel is equipped with a through-hole, the fixation kit is mounted in mounting base, the test tube is fixed below horizontal panel vertically by fixation kit, through-hole on the port alignment horizontal panel of the test tube, the loading component includes straight stick, the straight stick bottom is fixed with sample to be tested, the straight stick is extend into test tube by the through-hole on horizontal panel, it is placed in the sample to be tested for being fixed on straight stick bottom in test tube, realize loading operation from top to down.The hollow out setting that the invention passes through through hole on horizontal panel in mounting base, by being inserted into test tube from top to down of the straight stick in loading component, entire loading process realization not will receive any obstruction, and the sample to be tested position after guaranteeing loading is not in any deviation.
Description
Technical field
The present invention relates to sample fixing device and utilize optical projection tomography composed by the sample fixing device
System.
Background technique
Optical projection tomography technology (optical projection tomography, OPT) is a kind of new three-dimensional
Micro-imaging technique is the combination of microtechnic and CT technology, and optical projection tomography is to carry out multi-angle projection to sample,
Then digital technology reconstruction is reused, 3-D image is obtained.This imaging method has high-resolution, untouchable, reconstruction matter
Measure higher advantage.
There is the loading component of more optical projection tomography device at this stage, main difference is that biological sample is solid
Fixed difference, different biological samples will select its suitable fixed solution, fix as arabidopsis generallys use gel, and seed
Can using being fixed below turn table body after directly viscous glutinous, there are also some biological samples, for guarantee its bioactivity and
Good stretching after needing to be sticked and being fixed below turntable cylinder, then it is fixed by agar, is placed on refractive index
In match-pool.But these devices are the upper quadrat method using from the bottom up in operation, there is also because it is unsighted so that
The problem of loading sample position deviates.
Summary of the invention
The technical problem to be solved by the present invention is to：A kind of sampling device knot can be realized loading operation from top to bottom is provided
Structure.
The solution that the present invention solves its technical problem is：
A kind of sampling device structure, including mounting base, loading component, test tube and fixation kit, the mounting base include
Horizontal panel, the horizontal panel are equipped with a through-hole, and the fixation kit is mounted in mounting base, and the test tube passes through fixation
Component is fixed on vertically below horizontal panel, the through-hole on the port alignment horizontal panel of the test tube, the loading component packet
Straight stick is included, the straight stick bottom is fixed with sample to be tested, and the straight stick is extend into test tube by the through-hole on horizontal panel, made
The sample to be tested for being fixed on straight stick bottom is placed in test tube, realizes loading operation from top to down.
As a further improvement of the above technical scheme, the loading component further includes the adjusting for controlling straight stick lifting
Mechanism, the regulating mechanism are mounted in mounting base.
As a further improvement of the above technical scheme, the straight stick upper end is equipped with a toothed segment, the regulating mechanism packet
It includes accommodating cylinder, gear and the operating stick for controlling gear rotation, the gear and is placed in accommodating column body, the straight stick
Toothed segment be inserted into accommodating cylinder in engaged with gear, described operating stick one end is connect with gear, and the other end reaches accommodating
Outside cylinder.
As a further improvement of the above technical scheme, there are two the fixation kit settings, the fixation kit includes
Arc folder, sleeve and elastic mechanism, the sleeve are mounted below horizontal panel, and the elastic mechanism is arranged in sleeve
Portion, the arc folder are connected with elastic mechanism, and the test tube presss from both sides clamping by the arc of two fixation kits.
As a further improvement of the above technical scheme, the elastic mechanism include the first link block, the second link block with
And spring, first link block are fixed at sleeve inner, second link block is movably disposed in sleeve inner,
The both ends of the spring is connected with the first link block and the second link block respectively, and the arc folder is fixedly mounted with the second link block
Together.
As a further improvement of the above technical scheme, the fixation kit further includes rotating mechanism, the rotating mechanism
Including magnetic ball and magnetic commutation body, the magnetic commutation body is fixed below horizontal panel, the magnetic ball and magnetic commutation body
Magnetism connection, first link block are installed together with magnetic ball.
As a further improvement of the above technical scheme, the test tube is perfluoroethylene-propylene pipe, the refraction of the test tube
In 1.341~1.349 between rate.
The beneficial effect of the invention is：The invention is set by the hollow out of through hole on horizontal panel in mounting base
It sets, by being inserted into test tube from top to down of the straight stick in loading component, entire loading process realization not will receive any obstruction,
Sample to be tested position after guaranteeing loading is not in any deviation.
The invention also discloses a kind of optical projection tomography system simultaneously, including photographic device, light supply apparatus,
Frosted glass, turntable, motor, index matching pond and the sampling device, the motor driven turntable rotation, the camera shooting
Device, light supply apparatus, frosted glass and index matching pond are installed on turntable, and the test tube of the sampling device is placed in refraction
In rate match-pool, the light that the light supply apparatus is exported successively passes through frosted glass, index matching pond enters in photographic device.
The beneficial effect of the invention is：Imaging system described in the invention is guaranteed whole by the loading component
A loading process realization not will receive any obstruction, and the sample to be tested position after loading is not in any deviation, final to improve
The imaging effect of chromatographic imaging system, the sample to be tested 3-D image made are relatively sharp.
Detailed description of the invention
To describe the technical solutions in the embodiments of the present invention more clearly, make required in being described below to embodiment
Attached drawing is briefly described.Obviously, described attached drawing is a part of the embodiments of the present invention, rather than is all implemented
Example, those skilled in the art without creative efforts, can also be obtained according to these attached drawings other designs
Scheme and attached drawing.
Fig. 1 is the overall structure diagram of the invention；
Fig. 2 is the fixation kit structural schematic diagram of the invention；
Fig. 3 is the mounting base structural schematic diagram of the invention；
Fig. 4 is the loading component structure diagram of the invention.
Specific embodiment
It is carried out below with reference to technical effect of the embodiment and attached drawing to design of the invention, specific structure and generation clear
Chu, complete description, to be completely understood by the purpose of the present invention, feature and effect.Obviously, described embodiment is this hair
Bright a part of the embodiment, rather than whole embodiments, based on the embodiment of the present invention, those skilled in the art are not being paid
Other embodiments obtained, belong to the scope of protection of the invention under the premise of creative work.In addition, be previously mentioned in text
All connection/connection relationships not singly refer to that component directly connects, and referring to can be added deduct according to specific implementation situation by adding
Few couple auxiliary, to form more preferably coupling structure.Each technical characteristic in the invention, in not conflicting conflict
Under the premise of can be with combination of interactions.
Referring to Fig.1~Fig. 4, the invention disclose a kind of sampling device structure, including mounting base 1, loading component 2,
Test tube 3 and fixation kit 4, the mounting base 1 include horizontal panel 11, and the horizontal panel 11 is equipped with a through-hole 12, institute
It states fixation kit 4 to be mounted in mounting base 1, the test tube 3 is fixed on 11 lower section of horizontal panel, institute by fixation kit 4 vertically
The through-hole 12 on the port alignment horizontal panel 11 of test tube 3 is stated, the loading component 2 includes straight stick 21, and the straight stick 21 is preferential
It is made using aluminum material, straight 21 bottom of stick is fixed with sample to be tested, and the straight stick 21 passes through on horizontal panel 11
Through-hole 12 is extend into test tube 3, is placed in the sample to be tested for being fixed on straight 21 bottom of stick in test tube 3, realizes from top to down upper
Sample operation.Specifically, the invention is by the hollow out setting at through-hole 12 on horizontal panel 11 in mounting base 1, by loading group
Straight stick 21 in part 2 being inserted into test tube 3 from top to down, entire loading process realization not will receive any obstruction, in guarantee
Sample to be tested position after sample is not in any deviation.
It is further used as preferred embodiment, in the invention specific embodiment, the loading component 2 further includes
The regulating mechanism gone up and down for controlling straight stick 21, the regulating mechanism are mounted in mounting base 1, pass through setting for the regulating mechanism
It sets, convenient for users to the lifting of straight stick 21 can be controlled according to actual needs to control position of the sample to be tested in test tube 3.
Specifically, in the invention specific embodiment, straight 21 upper end of stick is equipped with a toothed segment 22, the adjusting
Mechanism includes accommodating cylinder 23, gear and the operating stick 24 for controlling gear rotation, and the gear is placed in accommodating cylinder 23
Inside, the toothed segment 22 of the straight stick 21 are inserted into accommodating cylinder 23 and engage with gear, described 24 one end of operating stick and gear
Connection, the other end reach outside accommodating cylinder 23, the lifting behaviour that user only has rotation operating stick 24 that straight stick 21 can be realized
Make.It should be noted that the through-hole referring to Fig. 3 and Fig. 4, in the invention specific embodiment, on the horizontal panel 11
12 be ladder hole 12, and the accommodating cylinder 23 of the regulating mechanism is designed as the structure to match with ladder hole 12, the appearance
It sets and is provided with one layer of rubber membrane between 23 outer peripheral surface of cylinder and through-hole 12, by the setting of the rubber membrane, make to accommodate cylinder 23
There are buffer shock-absorbing effects between horizontal panel 11, while can play and increase frictional force between accommodating cylinder 23 and through-hole 12
Effect.
It is further used as preferred embodiment, in the invention specific embodiment, the fixation kit 4 is provided with
Two, the fixation kit 4 includes that arc folder 41, sleeve 42 and elastic mechanism, the sleeve 42 are mounted on horizontal panel 11
Lower section, the elastic mechanism are arranged inside sleeve 42, and the arc folder 41 is connected with elastic mechanism, and the test tube 3 is by two
41 clamping of arc folder of a fixation kit 4.What sleeve 42 described in present embodiment was preferentially made using magnetic material, institute
11 bottom surface of horizontal panel is stated equipped with magnetic pole 13, the magnetic pole 13 is connect with 42 magnetism of sleeve to realize horizontal panel 11 and sleeve 42
Detachability installation, so that sleeve is securely disposed at the lower section of horizontal panel 4, guarantee that test tube 3 is clamped in strongerly
Between two arc folders；In addition, the folder of arc described in the invention 41 is to be mounted on 11 lower section of horizontal panel by elastic mechanism
, it is to need the test tube 3 where sample to be tested immersing refractive index for presently used imaging system, in use process
With in pond, index matching pond is placed in the center of turntable, rotates with the rotation of turntable, and liquid level is easily made to generate slight shake
It is dynamic, and water decline the stability of sample the resistance of sample in rotary course, make the data acquired there are biggish error,
So that sample image quality reduces, and the invention is by the setting of elastic mechanism, and in index matching pond, liquid level shakes
Play the role of buffer shock-absorbing when dynamic, improve the stability of imaging system, makes imaging effect more preferably.
Specifically, in the invention specific embodiment, the elastic mechanism is connected including the first link block 43, second
Block 44 and spring 45, first link block 43 are fixed inside sleeve 42, and second link block 44 is movably
It is arranged inside sleeve 42,45 both ends of spring are connected with the first link block 43 and the second link block 44 respectively, the arc
Clevis 41 is fixedly installed togather with the second link block 44.
It is further used as preferred embodiment, in the invention specific embodiment, the fixation kit 4 further includes
Rotating mechanism, the rotating mechanism include magnetic ball 46 and magnetic commutation body 47, and the magnetic commutation body 47 is removably installed
Below horizontal panel 11, the magnetic ball 46 is connect with 47 magnetism of magnetism commutation body, and first link block 43 is pacified with magnetic ball 46
It is fitted together.Specifically, in the invention specific embodiment, the magnetic commutation body 47 is actually one and removes continuously
The square body of two end faces, magnetism commutation body 47 can be mounted on 11 lower section of horizontal panel by screw, and the invention is by turning
41 rotation of arc folder is realized in the setting of motivation structure, is repelled each other using magnetic material like pole, the attracting principle of opposite pole, so that
It is motionless that arc folder 41 may remain in the position vertical with test tube 3, can also artificially make its 90 degree to rotate.Referring to Fig. 2, Fig. 2
The surface structure schematic diagram of fixation kit 4 is shown, from being can clearly see between magnetic ball 46 and magnetic commutation body 47 in Fig. 2
Positional relationship, in the fixation kit 4 shown in Fig. 1, the sleeve 42 is by transparence, to observe inside sleeve 42
Mechanical structure.
It is further used as preferred embodiment, in the invention specific embodiment, the test tube 3 is perfluoroethylene
Acrylic tubing, in 1.341~1.349 between the refractive index of the test tube 3, be effectively reduced refraction effect due to test tube 3 to chromatography at
As brought influence.
In addition the invention also discloses a kind of optical projection tomography system, including photographic device, light source simultaneously
Device, frosted glass, turntable, motor, index matching pond and above-described sampling device, the motor driven turntable turn
Dynamic, the photographic device, light supply apparatus, frosted glass and index matching pond are installed on turntable, the sampling device
Test tube 3 is placed in index matching pond, and the light that the light supply apparatus is exported successively passes through frosted glass, index matching pond enters
Into photographic device.Specifically, imaging system described in the invention guarantees entire loading mistake by the loading component 2
Cheng Shixian not will receive any obstruction, and the sample to be tested position after loading is not in any deviation, finally improve chromatography at
As the imaging effect of system, the sample to be tested 3-D image made is relatively sharp.
It is further used as preferred embodiment, in the invention specific embodiment, the imaging system further includes
Fixed frame, the sampling device are mounted below fixed frame, and 11 two sides of horizontal panel are respectively equipped with L-type connecting plate 13, institute
It states 13 top of L-type connecting plate and fixed frame is respectively arranged below with the permanent magnetic protrusion being mutually matched and magnetic recess,
Sampling device is fixed on to the stability for guaranteeing entire sampling device in operating process in some device by the fixed frame.
Better embodiment of the invention is illustrated above, but the invention is not limited to the implementation
Example, those skilled in the art can also make various equivalent modifications on the premise of without prejudice to spirit of the invention or replace
It changes, these equivalent variation or replacement are all included in the scope defined by the claims of the present application.
Claims (8)
1. a kind of sampling device structure, it is characterised in that：Including mounting base (1), loading component (2), test tube (3) and fixed group
Part (4), the mounting base (1) include horizontal panel (11), and the horizontal panel (11) is equipped with a through-hole (12), the fixation
Component (4) is mounted on mounting base (1), and the test tube (3) is fixed under horizontal panel (11) vertically by fixation kit (4)
Side, the port of the test tube (3) are directed at the through-hole (12) on horizontal panel (11), and the loading component (2) includes straight stick (21),
The straight stick (21) is extend into test tube (3) by the through-hole (12) on horizontal panel (11).
2. a kind of sampling device structure according to claim 1, it is characterised in that：The loading component (2) further includes using
In the regulating mechanism for controlling straight stick (21) lifting, the regulating mechanism is mounted on mounting base (1).
3. a kind of sampling device structure according to claim 2, it is characterised in that：Straight stick (21) upper end is equipped with a saw
Tooth section (22), the regulating mechanism include accommodating cylinder (23), gear and the operating stick (24) for controlling gear rotation, institute
It states gear and is placed in accommodating cylinder (23) inside, the toothed segment (22) of the straight stick (21) is inserted into accommodating cylinder (23) and gear
Engagement, described operating stick (24) one end are connect with gear, and it is external that the other end reaches accommodating cylinder (23).
4. a kind of sampling device structure according to claim 1, it is characterised in that：The fixation kit (4) is provided with two
A, the fixation kit (4) includes that arc folder (41), sleeve (42) and elastic mechanism, the sleeve (42) are mounted on level
Below panel (11), the elastic mechanism setting is internal in sleeve (42), and the arc folder (41) is connected with elastic mechanism, institute
State arc folder (41) clamping of the test tube (3) by two fixation kits (4).
5. a kind of sampling device structure according to claim 4, it is characterised in that：The elastic mechanism includes the first connection
Block (43), the second link block (44) and spring (45), first link block (43) are fixed at sleeve (42) inside, institute
Stating the second link block (44), to be movably disposed in sleeve (42) internal, spring (45) both ends respectively with the first link block
(43) and the second link block (44) is connected, and the arc folder (41) is fixedly installed togather with the second link block (44).
6. a kind of sampling device structure according to claim 5, it is characterised in that：The fixation kit (4) further includes turning
Motivation structure, the rotating mechanism include magnetic ball (46) and magnetic commutation body (47), and magnetic commutation body (47) is fixed on water
Below surface plate (11), the magnetic ball (46) connect with magnetism commutation body (47) magnetism, first link block (43) and magnetic ball
(46) it is installed together.
7. a kind of sampling device structure according to any one of claims 1 to 6, it is characterised in that：The test tube (3) is poly-
Perfluoroethylene-propylene (copolymer) pipe, in 1.341~1.349 between the refractive index of the test tube (3).
8. a kind of optical projection tomography system, it is characterised in that：Including photographic device, light supply apparatus, frosted glass, turntable,
Motor, index matching pond and the described in any item sampling devices of claim 1 to 7, the motor driven turntable rotation, institute
It states photographic device, light supply apparatus, frosted glass and index matching pond to be installed on turntable, the test tube of the sampling device
(3) it is placed in index matching pond, the light that the light supply apparatus is exported successively passes through frosted glass, index matching pond enters
In photographic device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810870777.1A CN108844897B (en) | 2018-08-02 | 2018-08-02 | Loading device structure and optical projection tomography system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810870777.1A CN108844897B (en) | 2018-08-02 | 2018-08-02 | Loading device structure and optical projection tomography system |
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| Publication Number | Publication Date |
|---|---|
| CN108844897A true CN108844897A (en) | 2018-11-20 |
| CN108844897B CN108844897B (en) | 2024-03-26 |
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