CN108840904A - A method of extracting selenoprotein from tobacco leaf - Google Patents
A method of extracting selenoprotein from tobacco leaf Download PDFInfo
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- CN108840904A CN108840904A CN201810766201.0A CN201810766201A CN108840904A CN 108840904 A CN108840904 A CN 108840904A CN 201810766201 A CN201810766201 A CN 201810766201A CN 108840904 A CN108840904 A CN 108840904A
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- selenoprotein
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
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- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
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- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
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- Fertilizers (AREA)
Abstract
The present invention discloses a kind of method that selenoprotein is extracted from tobacco leaf, and low manufacture cost, manufacturing technology is easily mastered, and the methods of is homogenized, exchanges heat, is centrifuged, filter and handles, and isolates tobacco leaf protein matter, under alkaline condition by selenoprotein（PH value is 10.5）Dissolution.Gsc constant temperature 10 minutes, then PH is adjusted to neutrality, and adjusting Se content is 5.0 grams every milliliter, yellow solution is made, low temperature deposit is used, equipment needed for this preparation method is simple, preparation condition is normal pressure, is easy to control, nontoxic, the relatively conventional method of this process, the recovery rate of selenoprotein rises to 1%, and selenium content 1500ug/g, tobacco leaf selenoprotein has immunological regulation and antioxidation.
Description
Technical field
The method that the present invention relates to a kind of to extract selenoprotein from tobacco leaf.
Background technique
After Nano-selenium leaf fertilizer sprays, the selenium containing 2500 micrograms or so in tobacco leaf therefrom can extract " cigarette with industry law
Leaf selenoprotein ", existing technical solution extract the low efficiency of selenoprotein from fresh leaf.
Tobacco leaf selenoprotein has immunological regulation and antioxidation.
Selenium is the essential trace elements of the human body, is adjusted to human immunity, is delayed senescence, and protect liver anticancer etc. has important work
With.Selenium is mainly enriched in protein in plant, is existed with selenomethionine and selenocystein or other forms, in tobacco leaf
Containing more rich available protein, sprays Nano-selenium leaf fertilizer and significantly improve protein Se content in tobacco leaf, tobacco leaf is contained
The Ru bisco albumen research of selenium has shown that selenizing protein to free radical is removed, protects erythrocyte hemolysis, and chemically liver loss is protected
For shield better than not selenoprotein, the former provides soluble protein.
Summary of the invention
To solve the above-mentioned problems, the invention discloses a kind of low manufacture cost, manufacturing technology is easily mastered, and is homogenized, is changed
The processing of the methods of heat, centrifugation, filtering, isolates tobacco leaf protein matter, and selenoprotein is molten under alkaline condition (pH value 10.5)
Solution.Gsc constant temperature 10 minutes, PH is then adjusted to neutrality, adjusting Se content is 5.0 grams every milliliter, and yellow solution, low temperature is made
Deposit is used, and specific technical solution is：
A method of extracting selenoprotein from tobacco leaf, it is characterised in that：Include the following steps：
Step 1：Take the step of selenium-rich tobacco leaf sprayed by nanometer selenium tobacco leaf fertilizer is by refiner homogenate；
Step 2：The step of carrying out de- fiber and heat exchange by heat-exchanger rig；
Step 3：The step of carrying out de- slag by tripod pendulum type batch centrifugal；
Step 4：The step of being filtered by butterfly centrifugal machine；
Step 5：The step of carrying out selenoprotein separation by tube centrifuge；
Step 6：The step of by blender and strainer being added to be collected selenoprotein and washing selenoprotein；
Step 7：The step of selenoprotein is dissolved under alkaline condition；
Step 8：The step of selenoprotein bottling is sterilized and finished product is made.
Preferably：In step 7, the alkaline condition is that pH value is 10.5, and constant temperature 10 divides in stainless steel reaction pot
Clock.
Preferably：The nanometer selenium tobacco leaf fertilizer of the sprinkling includes that parts by weight are respectively former 40-60 parts of selenium powder, potassium sulfate
35-45 parts, 24-26 parts of urea, 14-16 parts of glucose, 46 parts of vitamin C 44-, 40-60 parts of lauryl sodium sulfate.
Preferably：The nanometer selenium tobacco leaf fertilizer includes that parts by weight are respectively former 45 parts of selenium powder, 40 parts of potassium sulfate, urea
25 parts, 15 parts of glucose, 45 parts of vitamin C, 45 parts of lauryl sodium sulfate.
Preferably：The preparation method of the nanometer selenium tobacco leaf fertilizer is；Include the following steps：
Step 1：Former selenium powder and 150 milliliters of nitric acid dopes are weighed according to above-mentioned proportion, heating in container is fitted into and stirs
The step of to selenous acid solution, the warm temperature are 100 DEG C of 80-, and mixing time is 2-6 hours；
Step 2：Weigh obtained by step 1 120-200 milliliters of selenous acid solution, then take 800 milliliters of soft water 500- with it is above-mentioned
After stirring, the potassium sulfate of said ratio is added in the glucose of proportion, urea is stirred evenly to be allowed to sufficiently react and be obtained
The step of nano selenium fertilizer intermediate；
Step 3：The nano selenium fertilizer intermediate that step 2 obtains is stirred, the vitamin C of said ratio is then added
Be stirred, after then proceeding to stirring, be added said ratio lauryl sodium sulfate the step of.
Compared with the prior art, the present invention has the following advantages：
Equipment needed for this preparation method is simple, and preparation condition is normal pressure, is easy to control, nontoxic, this process phase
To conventional method, the recovery rate of selenoprotein rises to 1%, and selenium content 1500ug/g, tobacco leaf selenoprotein has immune adjust
Section and antioxidation.
Specific embodiment
The present invention is further described below with reference to embodiment.
A kind of method that selenoprotein is extracted from tobacco leaf of embodiment, it is characterised in that：Include the following steps：
Step 1：Take the step of selenium-rich tobacco leaf sprayed by nanometer selenium tobacco leaf fertilizer is by refiner homogenate；
Step 2：The step of carrying out de- fiber and heat exchange by heat-exchanger rig；
Step 3：The step of carrying out de- slag by tripod pendulum type batch centrifugal；
Step 4：The step of being filtered by butterfly centrifugal machine；
Step 5：The step of carrying out selenoprotein separation by tube centrifuge；
Step 6：The step of by blender and strainer being added to be collected selenoprotein and washing selenoprotein；
Step 7：The step of selenoprotein is dissolved under alkaline condition；
Step 8：The step of selenoprotein bottling is sterilized and finished product is made.
The alkaline condition is that pH value is 10.5, constant temperature 10 minutes in stainless steel reaction pot.
Preferably：Nanometer selenium tobacco leaf fertilizer include parts by weight be respectively former 40 parts of selenium powder, 35 parts of potassium sulfate, 24 parts of urea,
14 parts of glucose, 44 parts of vitamin C, 40 parts of lauryl sodium sulfate.
Step 3:Using the photosynthesis of plant, using targeting technology, the step of nanotechnology, slow release method；
The preparation method of the nanometer selenium tobacco leaf fertilizer is；Include the following steps：
Step 1：Former selenium powder and 150 milliliters of nitric acid dopes are weighed according to above-mentioned proportion, heating in container is fitted into and stirs
The step of to selenous acid solution, the warm temperature are 100 DEG C, and mixing time is 6 hours；
Step 2：200 milliliters of solution of selenous acid are weighed obtained by step 1, then takes the grape of 700 milliliters of soft water with said ratio
After stirring, the potassium sulfate of said ratio is added in sugar, urea is stirred evenly to be allowed to sufficiently react and be obtained in nano selenium fertilizer
The step of mesosome；
Step 3：The nano selenium fertilizer intermediate that step 2 obtains is stirred, the vitamin C of said ratio is then added
It is stirred, after then proceeding to stirring, the step of lauryl sodium sulfate of said ratio is added.
Compared with the prior art, the present invention has the following advantages：
Equipment needed for this preparation method is simple, and preparation condition is normal pressure, is easy to control, nontoxic, this process phase
To conventional method, the recovery rate of selenoprotein rises to 1%, and selenium content 1500ug/g, tobacco leaf selenoprotein has immune adjust
Section and antioxidation.
It is obvious to a person skilled in the art that invention is not limited to the details of the above exemplary embodiments, Er Qie
In the case where without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, the present embodiments are to be considered as illustrative and not restrictive, and the scope of the present invention is by appended power
Benefit requires rather than above description limits, it is intended that all by what is fallen within the meaning and scope of the equivalent elements of the claims
Variation is included within the present invention.Any label in claim should not be construed as limiting the claims involved.
Claims (5)
1. a kind of method for extracting selenoprotein from tobacco leaf, it is characterised in that：Include the following steps：
Step 1：Take the step of selenium-rich tobacco leaf sprayed by nanometer selenium tobacco leaf fertilizer is by refiner homogenate；
Step 2：The step of carrying out de- fiber and heat exchange by heat-exchanger rig；
Step 3：The step of carrying out de- slag by tripod pendulum type batch centrifugal；
Step 4：The step of being filtered by butterfly centrifugal machine；
Step 5：The step of carrying out selenoprotein separation by tube centrifuge；
Step 6：The step of by blender and strainer being added to be collected selenoprotein and washing selenoprotein；
Step 7：The step of selenoprotein is dissolved under alkaline condition；
Step 8：The step of selenoprotein bottling is sterilized and finished product is made.
2. a kind of method for extracting selenoprotein from tobacco leaf according to claim 1, it is characterised in that：In step 7,
The alkaline condition is that pH value is 10.5, constant temperature 10 minutes in stainless steel reaction pot.
3. a kind of method for extracting selenoprotein from tobacco leaf according to claim 1, it is characterised in that：The sprinkling
Nanometer selenium tobacco leaf fertilizer includes that parts by weight are respectively former 40-60 parts of selenium powder, 35-45 parts of potassium sulfate, 24-26 parts of urea, glucose
14-16 parts, 44-46 parts of vitamin C, 40-60 parts of lauryl sodium sulfate.
4. a kind of method for extracting selenoprotein from tobacco leaf according to claim 3, it is characterised in that：Described receives
Rice selenium tobacco leaf fertilizer includes that parts by weight are respectively former 45 parts of selenium powder, 40 parts of potassium sulfate, 25 parts of urea, 15 parts of glucose, vitamin C
45 parts, 45 parts of lauryl sodium sulfate.
5. a kind of method for extracting selenoprotein from tobacco leaf according to claim 3 or 4, it is characterised in that：It is described
The preparation method of nanometer selenium tobacco leaf fertilizer is；Include the following steps：
Step 1：Former selenium powder and 150 milliliters of nitric acid dopes are weighed according to above-mentioned proportion, heating in container is fitted into and stirs to get Asia
The step of selenic acid solution, the warm temperature are 80-100 DEG C, and mixing time is 2-6 hours；
Step 2：120-200 milliliters of selenous acid solution are weighed obtained by step 1, then takes 500-800 milliliters of soft water and said ratio
After stirring, the potassium sulfate of said ratio is added in glucose, urea stirs evenly to be allowed to sufficiently react and obtains nanometer selenium
The step of fertile intermediate；
Step 3：The nano selenium fertilizer intermediate that step 2 obtains is stirred, the vitamin C that said ratio is then added carries out
Stirring, after then proceeding to stirring, be added said ratio lauryl sodium sulfate the step of.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810766201.0A CN108840904B (en) | 2018-07-12 | 2018-07-12 | Method for extracting selenoprotein from tobacco leaves |
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| CN201810766201.0A CN108840904B (en) | 2018-07-12 | 2018-07-12 | Method for extracting selenoprotein from tobacco leaves |
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| CN108840904A true CN108840904A (en) | 2018-11-20 |
| CN108840904B CN108840904B (en) | 2022-03-04 |
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Citations (9)
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