CN108836984A - A kind of Cordyceps militaris leaching liquor and its preparation method and application and cordycepin beverage - Google Patents
A kind of Cordyceps militaris leaching liquor and its preparation method and application and cordycepin beverage Download PDFInfo
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- CN108836984A CN108836984A CN201810833832.XA CN201810833832A CN108836984A CN 108836984 A CN108836984 A CN 108836984A CN 201810833832 A CN201810833832 A CN 201810833832A CN 108836984 A CN108836984 A CN 108836984A
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- Prior art keywords
- leaching liquor
- cordyceps militaris
- preparation
- cordyceps
- cordycepin
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
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Classifications
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- A—HUMAN NECESSITIES
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- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
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Abstract
The present invention provides a kind of Cordyceps militaris leaching liquor and its preparation method and application and cordycepin beverages, belong to food and drink processing technique field.The preparation method of Cordyceps militaris leaching liquor provided by the invention is using cordyceps mycelium as raw material, and drying, crushing and water mentions twice obtain Cordyceps militaris leaching liquor.Preparation method provided by the invention is easy to operate, reproducible.Fibrinolytic enzyme activity has the function of good reverse thrombus tendency in 3~5U/mL in Cordyceps militaris leaching liquor produced by the present invention.
Description
Technical field
The invention belongs to food and drink manufacture fields, and in particular to a kind of Cordyceps militaris leaching liquor and its preparation method and application
And cordycepin beverage.
Background technique
Cordyceps militaris (Cordyceps militaris) also known as Cordceps militaris, are the type sepecies of Cordyceps, in taxonomy
Cordyceps (Cordyceps) is belonged to cordyceps sinensis (Cordyceps sinensis).Cordyceps militaris contains Cordyceps sinensis polysaccharide, cordyceps sinensis
The nutritional ingredients such as element, ucleosides, sterols and amino acid needed by human, vitamin and superoxide dismutase
Various bioactivators such as (superoxide dismutase, SOD) in adjustment central functions, extend sleep, anti-
Inflammation, anticancer, antibacterial, anti-aging etc. have good effects, are a kind of good nutrition and excellent tonic product.
In recent years, with the aging of social population and the continuous improvement of living standard, hyperlipemia, atherosclerosis
And the disease incidence for the heart, cerebrovascular thrombotic disease being evolved by it increases year by year, it seriously threatens the life of the mankind
And health, the disease incidence that disease incidence alreadys exceed cancer and diabetes leap to the first place of human death's reason.It is existing conventional
Thrombus medicine such as tissue-type plasminogen activator (t-PA), the mutant (reteplase Reteplase) of t-PA, rt-PA it is embedding
Zoarium etc., not only cost is high, and the course for the treatment of is long, and side effect is obvious, larger to lesions of liver and kidney, also easily causes the risks such as cerebral hemorrhage.
Therefore, of crucial importance is become to the prevention of thrombus disease.
Summary of the invention
The purpose of the present invention is to provide a kind of Cordyceps militaris leaching liquor and its preparation method and application and cordycepin beverages.
In order to achieve the above-mentioned object of the invention, the present invention provides following technical scheme：
The present invention provides a kind of preparation methods of Cordyceps militaris leaching liquor, include the following steps：
(1) by cordyceps mycelium drying, crushing, Cordyceps militaris powder is obtained；
(2) the Cordyceps militaris powder is mixed with water, extracts 1~3h, be separated by solid-liquid separation, obtain a supernatant and solid is residual
Slag；The quality of the Cordyceps militaris powder and the volume ratio of water are 1g:40~60ml；
(3) solid residue is mixed with water, extracts 1~3h, be separated by solid-liquid separation, obtain secondary supernatant；The solid is residual
The quality of slag and the volume ratio of water are 1g:20~30ml；
(4) supernatant and the secondary supernatant are merged, obtains Cordyceps militaris leaching liquor.
Preferably, the partial size that the step (1) crushes is 50~80 mesh.
Preferably, the step (2) or the middle temperature extracted of step (3) stand alone as 50~70 DEG C.
Preferably, the step (2) or the middle mode being separated by solid-liquid separation of step (3) are centrifugation, and the revolving speed of the centrifugation is independent
Time for 8000~12000r/min, centrifugation stands alone as 10~20min.
The present invention provides the Cordyceps militaris leaching liquor that above-mentioned preparation method is prepared, fibrinolytic in the Cordyceps militaris leaching liquor
The vigor of enzyme is 3~5U/mL.
The present invention provides a kind of with the cordycepin beverage for reversing thrombus tendency function, and the cordycepin beverage includes upper
The Cordyceps militaris leaching liquor stated, the volume fraction of Cordyceps militaris leaching liquor is 5%~90% in the cordycepin beverage.
Preferably, the cordycepin beverage further includes corn peptide, rose leaching liquor, black fungus leaching liquor and cassia seed leaching
One of extract is a variety of.
Preferably, the cordycepin beverage further includes auxiliary material；The auxiliary material is in honey, citric acid and ethylmaltol
It is one or more.
The present invention also provides application of the above-mentioned Cordyceps militaris leaching liquor in the drug of preparation prevention or treatment thrombus disease.
Beneficial effect：The present invention provides a kind of preparation methods of Cordyceps militaris leaching liquor, using cordyceps mycelium as raw material,
Drying, crushing and water mentions twice, have finally been made Cordyceps militaris leaching liquor.Preparation method provided by the invention is simple.It is obtained
Cordyceps militaris leaching liquor fibrinolytic enzyme activity has the function of good reverse thrombus tendency in 3~5U/mL.
The present invention also provides a kind of cordycepin beverage, in the cordycepin beverage include corn peptide, rose leaching liquor,
Function factor in black fungus leaching liquor or cassia seed leaching liquor not only has the function of good reverse thrombus tendency, also has
Anti-oxidant, protection cardiovascular and cerebrovascular, refreshment eyesight improving and other effects.
Detailed description of the invention
Fig. 1 is fibrin plate described in the embodiment of the present invention 2, and wherein Fig. 1-A indicates Cordyceps militaris leaching liquor by blood fibre
The transparent circle formed after fibrillarin dissolution, Fig. 1-B indicate the not formed transparent circle of saline control；
Fig. 2 is the HE colored graph of rabbit carotid artery cross section described in the embodiment of the present invention 2, wherein Fig. 2-A is that normal group is horizontal
Section, Fig. 2-B are experimental group cross section.
Specific embodiment
The present invention provides a kind of preparation methods of Cordyceps militaris leaching liquor, include the following steps：
(1) by cordyceps mycelium drying, crushing, Cordyceps militaris powder is obtained；
(2) the Cordyceps militaris powder is mixed in proportion with water, every 1g Cordyceps militaris powder mixture ratio 40~60mL water, extraction 1
~3h is separated by solid-liquid separation, obtains a supernatant and solid residue；
(3) solid residue being mixed in proportion with water, every 1g solid residue matches 20~30mL water, 1~3h is extracted,
It is separated by solid-liquid separation, obtains secondary supernatant；
(4) supernatant and the secondary supernatant are merged, obtains Cordyceps militaris leaching liquor.
The present invention preferably first dries cordyceps mycelium.In the present invention, the temperature of the drying is preferably 30~40
DEG C, more preferably 35 DEG C, at this temperature most beneficial for the activity of protection Cordyceps militaris fibrinolysin.The time of the drying is preferably
100~120min, more preferably 100min.The present invention is not particularly limited the source of the cordyceps mycelium, this field
The product of conventional commercial.The present invention is not particularly limited instrument used in the drying, and this field is conventionally used for drying
Dry tool.
After cordyceps mycelium after being dried, the present invention crushes the cordyceps mycelium after the drying,
Obtain Cordyceps militaris powder.In the present invention, the degree of the crushing is preferably 50~80 mesh, more preferably 60 mesh.The present invention couple
Crushing tool and the method for crushing are not particularly limited, the tool and method that this field is crushed conventionally used for food.
After obtaining Cordyceps militaris powder, the present invention mixes the Cordyceps militaris powder with water, will be in Cordyceps militaris using extraction
Effective component dissolve in water body.In the present invention, the mixed proportion of the Cordyceps militaris powder and water is preferably according to every 1g pupa worm
Careless 40~60mL of powder mixture ratio water, more preferably according to every 1g Cordyceps militaris powder mixture ratio 50mL water.The water temperature when extraction is preferred
It is 50~70 DEG C, more preferably 60 DEG C.The time of the extraction is 1~3h, preferably 2h.
After extraction, the present invention will be separated by solid-liquid separation, and obtain a supernatant and solid residue.In the present invention, described
The method of separation of solid and liquid preferably filters or centrifugation, is more preferably centrifuged.The revolving speed of the centrifugation is preferably 8000~12000r/
Min, more preferably 10000r/min.The time of the centrifugation is preferably 10~20min, more preferably 15min.
After obtaining a supernatant and solid residue, the present invention will mix the solid residue with water, using extraction,
Effective component remaining in solid residue is dissolved in water body.In the present invention, the mixed proportion of the solid residue and water is excellent
Choosing is according to every 1g Cordyceps militaris powder mixture ratio 20~30mL water, more preferably according to every 1g Cordyceps militaris powder mixture ratio 25mL water.The leaching
Water temperature when mentioning is preferably 50~70 DEG C, and more preferably 60 DEG C.The time of the extraction is 1~3h, preferably 2h.
After extraction, the present invention will be separated by solid-liquid separation, and obtain secondary supernatant.In the present invention, the separation of solid and liquid
Method preferably filters or centrifugation, is more preferably centrifuged.The revolving speed of the centrifugation is preferably 8000~12000r/min, more preferably
For 10000r/min.The time of the centrifugation is preferably 10~20min, more preferably 15min.
After obtaining secondary supernatant, the present invention merges a supernatant and the secondary supernatant, obtains pupa worm
Careless leaching liquor.After measured, the fibrinolytic enzyme activity in the Cordyceps militaris leaching liquor in 3~5U/mL there is good reverse thrombus to incline
To function.
The present invention provides a kind of with the cordycepin beverage for reversing thrombus tendency function, and the cordycepin beverage includes upper
State Cordyceps militaris leaching liquor.In the present invention, volume fraction of the Cordyceps militaris leaching liquor in the cordycepin beverage is preferably
5%~90%, more preferably 50%~75%.
In the present invention, for the cordycepin beverage it is also preferable to include other active materials, other described active materials are preferred
For one of corn peptide, rose leaching liquor, black fungus leaching liquor, cassia seed leaching liquor or a variety of.When the active material
When being two kinds, the active material preferably includes Cordyceps militaris leaching liquor and black fungus leaching liquor or Cordyceps militaris leaching liquor and Cassia
Sub- leaching liquor；When the active material is three kinds, the active material preferably includes corn peptide, rose leaching liquor and pupa worm
Careless leaching liquor.
In the present invention, the corn peptide is that have life by what protease hydrolytic obtained using corn protein powder as raw material
The active small-molecular peptides of object, can thoroughly be dissolved in water within the scope of very big pH, extremely stable to heat, securely and reliably, nontoxic pair
Effect is a kind of natural food protein.Rich in amino acid necessary to a variety of human bodies in corn peptide, and it is easy to human body suction
It receives.Special amino acid composition and connection type assign corn peptide multiple biological activities, such as antifatigue, promote alcohol metabolism, is anti-
Oxidation, anticancer etc..The present invention is not specially limited the source of the corn peptide, and this field conventional commercial corn peptide product is equal
It can.In the present invention, the corn peptide addition quality preferably accounts for the 0.5~2% of the cordycepin beverage gross mass, more preferably
1%.
In the present invention, the function of the rose leaching liquor is the peculiar smell and the sense of taste covered in the smell of corn peptide bring
On bitter taste, while also having effects that oxidation resistant.The present invention does not limit the preparation method of the rose leaching liquor especially
Fixed, the conventional water that rose is applied to food and drink processing in this field proposes method.In the present invention, the rose leaching
The additive amount of extract is preferably 1/3~2/3 times of the Cordyceps militaris leaching liquor volume, more preferably 1/2 times.
In the present invention, the black fungus leaching liquor nutriment is abundant, not only rich in needed by human body such as calcium, phosphorus, iron
Mineral matter element, also containing amino acid necessary to lecithin, cephalin and human body.The present invention is to the black fungus leaching liquor
Preparation method is not particularly limited, and the conventional water that black fungus is applied to food and drink processing in this field proposes method.At this
In invention, the additive amount of the black fungus leaching liquor is preferably 1/5~2/5 times of the Cordyceps militaris leaching liquor volume, more preferably
It is 1/3 times.
In the present invention, the cassia seed leaching liquor is full of nutrition, removes and contains polysaccharide, protein, fatty acid, inorganic member
Element, amino acid, outside vitamin, also containing Chrysophanol, obtusin, Anthraquinones, non-saponified substance, naphtho--pyranone etc. at
Point.The present invention is not particularly limited the preparation method of the cassia seed leaching liquor, and this field is conventional to be applied to food for cassia seed
The water of product beverage processing proposes method.In the present invention, the additive amount of the cassia seed leaching liquor is preferably the Cordyceps militaris
1/5~2/5 times of leaching liquor volume, more preferably 1/3 times.
In the present invention, for the cordycepin beverage it is also preferable to include auxiliary material, the auxiliary material is honey, citric acid, ethyl wheat
One of bud phenol is a variety of.When the auxiliary material is two kinds, the auxiliary material preferably includes honey and citric acid；When the activity
When substance includes corn peptide, the auxiliary material preferably includes ethylmaltol.
In the present invention, the honey also contains a small amount of vitamin, amino acid, albumen other than rich in glucide
Matter, organic acid and a variety of the essential trace elements of the human bodys.In addition, also containing in honey, there are many enzyme materials, mainly include grape
Carbohydrate oxidase, amylase and invertase etc., wherein sucrose inversion in honey can be to have active monosaccharide, i.e. α-by invertase
Fructose and phlorose.In the present invention, honey addition quality preferably account for the cordycepin beverage gross mass 3~
10%, more preferably 8%.
In the present invention, the citric acid is used to adjust the acidity of cordycepin beverage.The citric acid addition quality is preferred
Account for the 0.005~0.02% of the cordycepin beverage gross mass, more preferably 0.01~0.015%.
In the present invention, the ethylmaltol is used to promote the fragrant degree of cordycepin beverage, remove corn peptide or other at
The bitter stink divided.The corn peptide addition quality preferably accounts for the 0.05~0.15% of the cordycepin beverage gross mass, more preferably
It is 0.1%.
The present invention also provides application of the above-mentioned Cordyceps militaris leaching liquor in the drug of preparation prevention or treatment thrombus disease.
Using above-mentioned Cordyceps militaris leaching liquor as sole active agent, or with above-mentioned Cordyceps militaris leaching liquor cooperation other active components, use this
Field acceptable carrier can be prepared by prevention or treatment thrombus of the present invention using this field conventional medicine preparation method
The drug of disease.In the present invention, other described medicinal active ingredients include fungi polysaccharide, cordycepin, small active peptides, class
The quality proportioning of chromocor compound etc., other medicinal active ingredients and Cordyceps militaris leaching liquor is 0~100:1.
A kind of Cordyceps militaris leaching liquor provided by the invention and its preparation method and application is carried out below with reference to embodiment detailed
Thin explanation, but they cannot be interpreted as limiting the scope of the present invention.
Embodiment 1
It by cordyceps mycelium drying, crushes, 60 meshes is crossed, in 60 DEG C, solid-liquid ratio 1:Flooding 2h is used under the conditions of 50, it is cold
But, 10000r/min is centrifuged 15min, supernatant is obtained, by solid-liquid ratio 1:25 water is added into precipitating, soaks again under the same terms
2h is mentioned, cooling, 10000r/min is centrifuged 15min, obtains supernatant, supernatant will merge twice, as Cordyceps militaris leaching liquor.
Embodiment 2
(1) prove that Cordyceps militaris leaching liquor has fibrinolytic effect using fibrin plate method.
Fibrin plate contains fibrinogen and fibrin ferment, and soluble fibrinogen is in fibrin ferment
Fibrin monomer, the spontaneous conclusion of fibrin monomer are formed under effect, multimerization forms insoluble macroscopic blood fibre
Cordyceps militaris leaching liquor described in embodiment 1 is added to this slab gel surface, after the short time cultivates by fibrillarin gel
By fibrinolysis, macroscopic transparent solusphere is formed on slab gel surface.
The preparation of fibrin plate：For preparing 10 plates, 0.25g agarose is dissolved in 50mL physiological saline
In, after being placed in 45 DEG C of water-bath heat preservation 30min, the fibrin ferment for adding 200U is mixed；0.2g bovine fibrinogen is dissolved in a bar ratio
Appropriate sodium buffer (pH7.8), is placed in 45 DEG C of water-baths and keeps the temperature 5min, takes 5mL fibrinogen solution and 5mL fibrin ferment molten respectively
Liquid mixing pour into plate (diameter 9cm), be put into after horizontal rest 30min 4 DEG C it is spare.
The preparation of fibrinolysin enzyme activity standard curve：The preparation of urokinase standard items, the urokinase physiology that 50000U/ is propped up
Salt water is diluted to 30,25,20,15,10,5,1U/mL storage it is spare.The urokinase standard items of each concentration take 10uL × 3 point flat
Plate, 37 DEG C of heat preservation 6h, amount diameter are averaged.Using x as solusphere diameter (cm), y is regression equation y=obtained by enzymatic activity (U/mL)
0.1219e4.6981x, R2=0.978.
Cordyceps militaris leaching liquor enzyme activity determination：Take 10 μ L Cordyceps militaris leaching liquor points flat in fibrin with micropipettor
Plate surface, each in parallel in triplicate, the transparent solusphere diameter (being detailed in Fig. 1) that assay plate surface is formed after 37 DEG C of heat preservation 6h,
Using physiological saline as control, enzyme activity can be calculated by bringing solusphere diameter into formula.
In Fig. 1, Fig. 1-A represents the transparent circle that Cordyceps militaris leaching liquor will be formed after fibrinolysis, and Fig. 1-B is represented
The not formed transparent circle of saline control.Fig. 1 shows that the Cordyceps militaris leaching liquor that the present invention is prepared has good reverse blood
The function of bolt tendency.
(2) Cordyceps militaris leaching liquor feeding rat suppository described in present invention embodiment 1 is inclined to animal model, rat adaptability
Feed after a week, Direct-fed high lipid food 17d, carry out modeling, normal group feeds basal feed, model group feed high lipid food and
Drinking water 24 days, normal group fed basal feed and drinking water 24 days, and experimental group feeds high lipid food and after drinking water 17 days, then
Feed basal feed and Cordyceps militaris leaching liquor 7 days.24 days fasting for solids and liquids at night, put to death in 25 days, takes cardiac blood, measures in blood plasma
Each index content.Prove that animal model can be effectively reduced in Cordyceps militaris fibrinolysin by rat suppository tendency animal model test
Total cholesterol, triglyceride and High-density Lipoprotein-cholesterol in serum, and low density lipoprotein cholesterol can be increased and contained
Amount；It is effective to extend thrombin time of blood plasma, prothrombin time, activated partial thromboplastin time, while reducing fiber
Proteinogen content；Reduce plasminogen activator inhibitor and activities of endothelial tissue plasminogen content, raise simultaneously tissue plasmin swash
Agent active quantities and plasmin activity living, illustrate its hyperlipemia that can effectively prevent and thrombus is reversed to be inclined to.From interior
Dirty index is seen, compared with being inclined to model group with thrombus, Cordyceps militaris fibrinolysin has certain protective effect to viscera in rats.
(3) Cordyceps militaris leaching liquor described in present invention embodiment 1 feeds Arteries of Rabbits thrombus model, and experiment uses normal family
Rabbit, normal group to its stomach-filling physiological saline, experimental group stomach-filling Cordyceps militaris leaching liquor.After last stomach-filling 2h, by improveing hanging wire
The foundation of method progress Arteries of Rabbits thrombus model.After experiment, put to death rabbit, production rabbit carotid artery slice, and to its into
Row pathological observation, as a result as shown in Figure 2.Fig. 2 the result shows that, normal organize is full of thrombus in rabbit carotid artery lumen, intravascular
Elastic membrane damage is serious, and there are thrombus in Cordyceps militaris experimental group rabbit carotid artery lumen, but has part to Arteries of Rabbits thrombus
Softening, absorbs dissolution, and endangium has no obvious damage, illustrates that it has prevention effect to thrombosis.
Embodiment 3
It by cordyceps mycelium drying, crushes, 60 meshes is crossed, in 60 DEG C, solid-liquid ratio 1:Flooding 2h is used under the conditions of 50, it is cold
But, 10000r/min is centrifuged 15min, supernatant is obtained, by solid-liquid ratio 1:25 water is added into precipitating, soaks again under the same terms
2h is mentioned, cooling, 10000r/min is centrifuged 15min, obtains supernatant, supernatant will merge twice, as Cordyceps militaris leaching liquor.
It takes 1g rose that 500mL water is added and extracts 30min at 80 DEG C, rose leaching liquor is made.
According to Cordyceps militaris leaching liquor:Rose wate=2:1 volume ratio mixes Cordyceps militaris leaching liquor and rose leaching liquor
It closes, corn peptide, ethylmaltol, honey and citric acid is then added, be made with the Cordyceps militaris drink for reversing thrombus tendency function
Material.Wherein, on the basis of the cordycepin beverage, the mass percent of each component addition is as follows：1% corn peptide, 0.1% ethyl
Maltol, 8% honey, 0.015% citric acid.
After measured, the fibrinolytic enzyme activity in the cordycepin beverage is 2.78U/mL；Cordycepin content is 12.43 μ g/mL；
Polyoses content is 199mg/mL；For DPPH free radical scavenging ability, it is approximately equivalent in every milliliter of beverage containing 0.3mg/mL's
Vc；For OH free radical scavenging ability, the Vc containing 0.42mg/mL is approximately equivalent in every milliliter of beverage；For reducing power energy
Power is approximately equivalent to the Vc containing 0.25mg/mL in every milliliter of beverage.Beverage product obtained has thrombus, anti-oxidant, anti-
Fatigue improves and exempts from physical strength and other effects.
Embodiment 4
It by cordyceps mycelium drying, crushes, 60 meshes is crossed, in 60 DEG C, solid-liquid ratio 1:Flooding 2h is used under the conditions of 50, it is cold
But, 10000r/min is centrifuged 15min, supernatant is obtained, by solid-liquid ratio 1:25 water is added into precipitating, soaks again under the same terms
2h is mentioned, cooling, 10000r/min is centrifuged 15min, obtains supernatant, supernatant will merge twice, as Cordyceps militaris leaching liquor.
Dry black fungus is taken to be sent out with bubble, removal root is cleaned, by dry weight 1:200 are homogenized, by weight in wet base 1:5 at 60 DEG C
2h is extracted, cooling, 10000r/min is centrifuged 15min, supernatant is obtained, by solid-liquid ratio 1:2.5 water, identical item are added into precipitating
2h is extracted under part again, cooling, 10000r/min is centrifuged 15min, obtains supernatant, supernatant will merge twice, as black wood
Ear leaching liquor.
According to Cordyceps militaris leaching liquor：Black fungus leaching liquor=3:1 volume ratio extracts Cordyceps militaris leaching liquor and black fungus
Liquid mixing, is then added ethylmaltol, honey and citric acid, is made with the cordycepin beverage for reversing thrombus tendency function.
Wherein, on the basis of the cordycepin beverage, the mass percent of each component addition is as follows：0.1% ethylmaltol, 8%
Honey, 0.015% citric acid.
After measured, the fibrinolytic enzyme activity in the cordycepin beverage is 4.28U/mL；Cordycepin content is 9.32 μ g/mL；
Polyoses content is 180mg/mL；For DPPH free radical scavenging ability, it is approximately equivalent in every milliliter of beverage containing 0.03mg/mL's
Vc；For OH free radical scavenging ability, the Vc containing 0.42mg/mL is approximately equivalent in every milliliter of beverage；For reducing power energy
Power is approximately equivalent to the Vc containing 0.188mg/mL in every milliliter of beverage.Beverage product obtained have thrombus, it is anti-oxidant,
Physical strength and other effects is exempted from anticancer, raising.
Embodiment 5
It by cordyceps mycelium drying, crushes, 60 meshes is crossed, in 60 DEG C, solid-liquid ratio 1:Flooding 2h is used under the conditions of 50, it is cold
But, 10000r/min is centrifuged 15min, supernatant is obtained, by solid-liquid ratio 1:25 water is added into precipitating, soaks again under the same terms
2h is mentioned, cooling, 10000r/min is centrifuged 15min, obtains supernatant, supernatant will merge twice, as Cordyceps militaris leaching liquor.
The cassia seed after frying dries pulverizing is taken, in 60 DEG C, solid-liquid ratio 1:Flooding 2h is used under the conditions of 20, it is cooling,
10000r/min is centrifuged 15min, supernatant is obtained, by solid-liquid ratio 1:10 water is added into precipitating, extracts again under the same terms
2h, cooling, 10000r/min is centrifuged 15min, obtains supernatant, supernatant will merge twice, as cassia seed leaching liquor.
According to Cordyceps militaris leaching liquor：Cassia seed leaching liquor=3:1 volume ratio extracts Cordyceps militaris leaching liquor and cassia seed
Liquid mixing, is then added honey and citric acid, is made with the cordycepin beverage for reversing thrombus tendency function.Wherein, with described
On the basis of cordycepin beverage, the mass percent of each component addition is as follows：8% honey, 0.01% citric acid.
After measured, the fibrinolytic enzyme activity in the cordycepin beverage is 3.09U/mL；Cordycepin content is 9.32 μ g/mL；
Polyoses content is 189mg/mL；For DPPH free radical scavenging ability, it is approximately equivalent in every milliliter of beverage containing 0.03mg/mL's
Vc；For OH free radical scavenging ability, the Vc containing 0.2mg/mL is approximately equivalent in every milliliter of beverage；For reducing power energy
Power is approximately equivalent to the Vc containing 0.188mg/mL in every milliliter of beverage.Beverage product obtained have thrombus, it is anti-oxidant,
Physical strength and other effects is exempted from antifatigue, raising.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (9)
1. a kind of preparation method of Cordyceps militaris leaching liquor, which is characterized in that include the following steps：
(1) by cordyceps mycelium drying, crushing, Cordyceps militaris powder is obtained；
(2) the Cordyceps militaris powder is mixed with water, extracts 1~3h, be separated by solid-liquid separation, obtain a supernatant and solid residue；Institute
The volume ratio of the quality and water of stating Cordyceps militaris powder is 1g:40~60mL；
(3) solid residue is mixed with water, extracts 1~3h, be separated by solid-liquid separation, obtain secondary supernatant；The solid residue
Quality and the volume ratio of water are 1g:20~30mL；
(4) supernatant and the secondary supernatant are merged, obtains Cordyceps militaris leaching liquor.
2. preparation method according to claim 1, which is characterized in that the partial size of step (1) the Cordyceps militaris powder is 50
~80 mesh.
3. preparation method according to claim 1, which is characterized in that the temperature extracted in the step (2) or step (3)
Stand alone as 50~70 DEG C.
4. preparation method according to claim 1, which is characterized in that be separated by solid-liquid separation in the step (2) or step (3)
Mode is centrifugation, and the revolving speed of the centrifugation stands alone as 8000~12000r/min, and the time of centrifugation stands alone as 10~20min.
5. the Cordyceps militaris leaching liquor that any one of Claims 1 to 4 preparation method is prepared, which is characterized in that the pupa
The vigor of fibrinolysin is 3~5U/mL in cordyceps sinensis leaching liquor.
6. a kind of with the cordycepin beverage for reversing thrombus tendency function, which is characterized in that the cordycepin beverage includes right
It is required that Cordyceps militaris leaching liquor described in 5, the volume fraction of Cordyceps militaris leaching liquor is 5%~90% in the cordycepin beverage.
7. cordycepin beverage according to claim 6, which is characterized in that the cordycepin beverage further includes corn peptide, rose
One of rare colored leaching liquor, black fungus leaching liquor and cassia seed leaching liquor are a variety of.
8. cordycepin beverage according to claim 6 or 7, which is characterized in that the cordycepin beverage further includes auxiliary material；Institute
Stating auxiliary material is one of honey, citric acid and ethylmaltol or a variety of.
9. application of the Cordyceps militaris leaching liquor described in claim 5 in the drug of preparation prevention or treatment thrombus disease.
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