CN108828759B - Microscopic imaging device with continuously adjustable magnification - Google Patents
Microscopic imaging device with continuously adjustable magnification Download PDFInfo
- Publication number
- CN108828759B CN108828759B CN201810555920.8A CN201810555920A CN108828759B CN 108828759 B CN108828759 B CN 108828759B CN 201810555920 A CN201810555920 A CN 201810555920A CN 108828759 B CN108828759 B CN 108828759B
- Authority
- CN
- China
- Prior art keywords
- lens
- convex
- surface facing
- object side
- lens group
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/24—Base structure
- G02B21/241—Devices for focusing
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Microscoopes, Condenser (AREA)
Abstract
The invention relates to the field of microscopic imaging, and provides a microscopic imaging device with continuously adjustable magnification. The device comprises a coaxial light source, a sample area, a front module, a relay module, a rear module and an imaging area which are sequentially arranged; the relay module and the rear module can adjust the amplification factor of the whole device by adjusting the relative position of the movable lens. The experimental device has an open sample space, and can effectively control the visual field range and acquire experimental data with different resolutions by continuously adjusting the integral magnification.
Description
Technical Field
The invention relates to a microscopic imaging device, in particular to a microscopic imaging device with continuously adjustable magnification, and belongs to the field of microscopic imaging.
Background
Microscopic imaging has become the main means of sample observation at present, and has the advantages that a micrometer-scale sample can be observed, and a light source is introduced for optical test. The traditional optical microscope needs to achieve discontinuous adjustment of magnification by replacing an objective lens, has small observation space, is suitable for closely observing a sample with small volume, and cannot observe an object with large volume.
Disclosure of Invention
In view of the above, the invention provides a microscopic imaging device with continuously adjustable magnification, which has the advantages of flexible structure, large observation area space, continuously adjustable magnification range, adjustable visual field range, simple and quick operation process, and the like.
The microscopic imaging device with continuously adjustable magnification comprises: the device comprises a coaxial light source, an imaging area, a sample area, a front module, a relay module and a rear module, wherein the sample area, the front module, the relay module and the rear module are sequentially arranged between the coaxial light source and the imaging area;
the coaxial light source is used for providing uniform illumination;
the sample to be imaged is arranged in a sample area in front of the coaxial light source;
the front module comprises: a first lens group and a second lens coaxially arranged in order from an object side to an image side; the first lens group comprises more than one lens, and the whole lens is equivalent to a convex-concave lens with a convex surface facing to the object side and a concave surface facing to the image side; the second lens is a concave-convex lens with a concave surface facing the object side and a convex surface facing the image side; the first lens group is fixed in position, and the second lens can translate along the axial direction of the second lens group;
the relay module includes: a third lens and a fourth lens coaxially arranged in order from the object side to the image side; wherein the third lens is a biconcave lens; the fourth lens is a plano-convex lens with a convex surface facing the image side; the third lens is fixed in position, and the fourth lens can translate along the axial direction of the fourth lens;
the rear module includes: a fifth lens group and a sixth lens coaxially arranged in order from the object side to the image side; the fifth lens group comprises more than one lens, the whole lens is equivalent to a plano-convex lens with a convex surface facing to the object side, and the sixth lens is a concave-convex lens with a concave surface facing to the object side and a convex surface facing to the image side; the position of the fifth lens group is fixed, and the sixth lens can translate along the axial direction of the fifth lens group;
and the imaging area photographs the sample to be detected through a camera.
Advantageous effects
(1) The microscopic imaging device provided by the invention can adjust the magnification (realized by moving the second lens group and the fourth lens) according to the size of an experimental sample, and adjust and control an observation area; and the position of the lens is adjusted to realize the continuous magnification adjustment of the imaging device, the experimental visual field range is adjusted, and the operation is simple, rapid and flexible.
(2) The device provided by the invention has an open observation space, and can realize microscopic observation of large samples, such as the appearance of combustion in a bomb, the flow state in a nozzle of an oil sprayer, nanowires and the like.
Drawings
FIG. 1 is a schematic view of a microscopic imaging apparatus of the present invention with continuously adjustable magnification;
in fig. 2, a is a schematic view of the internal flow of the transparent nozzle to be observed under low magnification, and B is a schematic view of the internal flow of the transparent nozzle to be observed under high magnification.
Wherein: 1-light source, 2-oil injector, 3-nozzle, 4-front module, 41-first lens group, 42-second lens, 43-slide rail A, 5-relay module, 51-third lens, 52-fourth lens, 53-slide rail B, 6-rear module, 61-fifth lens group, 62-sixth lens, 63-slide rail C, 7-camera
Detailed Description
The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings, and the embodiments described herein are only one of the embodiments, and do not represent all of the embodiments.
The present embodiment provides a microscopic imaging apparatus with continuously adjustable magnification, as shown in fig. 1, the microscopic imaging apparatus includes: the device comprises a coaxial light source 1, an imaging area, a sample area, a front module 4, a relay module 5 and a rear module 6 which are sequentially arranged between the coaxial light source 1 and the imaging area.
Wherein the coaxial light source 1 is used as a backlight source to provide uniform illumination for the device.
In the embodiment, an oil sprayer 2 is arranged in the sample area, a tip nozzle 3 of the oil sprayer is made of organic glass, a sample to be imaged is an oil beam within 0-2cm of the beginning of the tip nozzle 3 of the oil sprayer and fluid in a transparent nozzle, and the development condition of the oil beam within 0-2cm of the beginning of the nozzle 3, the flow state change in the transparent nozzle and the combustion of the oil beam can be obtained.
The front module 4 is used for converging light beams emitted by the coaxial light source 1, the front module 4 can reach different focal lengths and numerical apertures through different lens combinations, and the front module 4 comprises: the front lens module 4 includes a first lens group 41 and a second lens group 42 coaxially disposed in order from an object side (a sample area side) to an image side (an imaging area side). Wherein the first lens group 41 includes more than one lens, which is globally equivalent to a convex-concave lens with a convex surface facing the object side and a concave surface facing the image side (e.g., a single convex-concave lens with a convex surface facing the object side and a concave surface facing the image side or a lens group consisting of one convex-concave lens with a convex surface facing the object side and a concave surface facing the image side and one biconvex lens with two convex surfaces of different curvatures); the second lens element 42 is a meniscus lens element with a concave surface facing the object side and a convex surface facing the image side. The first lens group 41 is fixed, the second lens 42 is connected to the slide rail a43, and can slide along the slide rail a43, so as to change the relative position with the first lens 41, and the focal length of the front module 4 in this embodiment ranges from 30mm to 50 mm.
The relay module 5 includes: a relay lens module and a slide rail B53; the relay lens module includes, in order from the object side to the image side, a third lens 51 and a fourth lens 52 coaxially disposed. Wherein the third lens 51 is a biconcave lens; the fourth lens 52 is a plano-convex lens with a convex surface facing the image side; the third lens 51 is fixed in position; the fourth lens 52 is connected to the slide rail B53 and can slide along the slide rail B53 to change the relative position with the third lens 51.
The rear module 6 comprises a rear lens module and a slide rail C63; the rear lens module is composed of a fifth lens group 61 and a sixth lens group 62 which are coaxially arranged from the object side to the image side. The fifth lens group 61 includes more than one lens, and the whole lens is equivalent to a plano-convex lens with a convex surface facing the object side (for example, a single plano-convex lens with a convex surface facing the object side or a lens group consisting of one plano-convex lens with a convex surface facing the object side and one biconvex lens with two convex surfaces with different curvatures); the sixth lens element 62 is a meniscus lens element with a concave surface facing the object side and a convex surface facing the image side. The fifth lens group 61 is fixed in position; the sixth lens 62 is connected to a slide rail C63 and can slide along a slide rail C63 to change the relative position with the fifth lens group 61.
The imaging area is provided with a high-speed camera 7, a three-dimensional displacement table and a control unit for controlling the three-dimensional displacement table; the high-speed camera 7 is arranged on the three-dimensional displacement table and used for controlling the displacement of the high-speed camera 7 in the X, Y and Z directions (wherein the axial direction of each lens is the X direction, the vertical direction is the Z direction, and the direction vertical to the XZ plane is the Y direction).
The working principle of the microscopic imaging device is as follows: the coaxial light source 1 irradiates the transparent nozzle 3, the nozzle 3 adopts organic glass with the light transmittance of 92 percent, and the light loss is negligible. Due to refraction and scattering of the light, the bubbles in the nozzle 3 block the light, appearing dark on the high speed camera 7 image, and the liquid oil portion appears bright on the camera.
Light beams emitted by the light source 1 are converged by the first lens group 41 after passing through a sample, the converged light beams are changed into divergent light beams after passing through a focus of the first lens group 41, and then converged by the second lens group 42, so that the divergence angle of the light beams is reduced, but the light beams are still divergent light beams and then are emitted to the relay module 5. The front module 4 can achieve different focal lengths and numerical apertures through different lens combinations, and the lens assembly 4 in this embodiment achieves a focal length of 30-50 mm.
The divergent light emitted from the front module 4 is diverged by the third lens 51 in the relay module 5, and the central part beam of the divergent light is converged by the fourth lens 52 and emitted to the rear module 6.
The light beam emitted by the relay module 5 is converged by the fifth lens group 61 in the rear module 6, and the converged light beam is slightly diverged by the sixth lens 62, so that the focal plane position is extended. The equivalent focal length of the fifth lens group 61 and the sixth lens 62 in the rear module 6 is 180mm, and the numerical aperture f is 3.5.
When the second lens 42 in the front module 4 moves backward, the central beam enters the third lens 51, the edge part cannot enter the third lens, the magnification is increased, and on the contrary, the magnification is reduced when the second lens moves forward; the emergent light beam from the third lens 51 is incident on the fourth lens 52, and when the fourth lens 52 moves forward, the central light beam incident on the fifth lens group 61 is increased, and the magnification is reduced; on the contrary, the magnification is increased when the lens is moved backwards; when the focal plane position changes with magnification, the focal plane position can be changed by moving the sixth lens 62, and the focal plane is moved to the position where the camera chip is located.
In the above described device, all movable lenses have a unique degree of freedom (axial movement).
In the device, a high-speed camera 7 is selected for imaging, and high-time resolution data of the sample is obtained. High speed acquisition and short exposure times result in a reduction in the number of photons acquired by the camera, making the image darker. The front module, the relay module and the rear module are all provided with high-transmittance lenses, so that light loss is reduced, and luminous flux is increased. The microscopic imaging device can acquire experimental data and images with more details, and is applied to the aspects of observing the appearance of a sample, fuel development, cavitation formation and the like. The magnification of the imaging device is adjustable, and the adjusting range is 5-20 times.
Although the embodiments of the present invention have been described in conjunction with the accompanying drawings, it will be apparent to those skilled in the art that various changes and modifications may be made without departing from the principles of the invention, and these should be considered to fall within the scope of the invention.
Claims (7)
1. A microscopic imaging apparatus with continuously adjustable magnification, comprising: the device comprises a coaxial light source (1), an imaging area, a sample area, a front module (4), a relay module (5) and a rear module (6), wherein the sample area, the front module (4), the relay module (5) and the rear module are sequentially arranged between the coaxial light source (1) and the imaging area;
the coaxial light source (1) is used for providing uniform illumination;
the sample to be imaged is arranged in a sample area in front of the coaxial light source (1);
the front module (4) comprises: a first lens group (41) and a second lens (42) coaxially arranged in order from an object side to an image side; the first lens group (41) comprises more than one lens, and the whole lens is equivalent to a convex-concave lens with a convex surface facing to the object side and a concave surface facing to the image side; the second lens (42) is a concave-convex lens with a concave surface facing the object side and a convex surface facing the image side; the first lens group (41) is fixed in position, and the second lens (42) can translate along the axial direction thereof;
the relay module (5) comprises: a third lens (51) and a fourth lens (52) coaxially disposed in order from the object side to the image side; wherein the third lens (51) is a biconcave lens; the fourth lens (52) is a plano-convex lens with a convex surface facing the image side; the third lens (51) is fixed in position, and the fourth lens (52) can translate along the axial direction of the third lens;
the rear module (6) comprises: a fifth lens group (61) and a sixth lens (62) coaxially arranged in order from the object side to the image side; the fifth lens group (61) comprises more than one lens, the whole lens is equivalent to a plano-convex lens with a convex surface facing to the object side, and the sixth lens (62) is a concave-convex lens with a concave surface facing to the object side and a convex surface facing to the image side; the fifth lens group (61) is fixed in position, and the sixth lens (62) can translate along the axial direction of the fifth lens group;
the imaging area photographs the sample to be measured through a camera (7).
2. The continuously variable magnification microscopic imaging apparatus according to claim 1, wherein the second lens group (42) is disposed on a slide a (43) and is axially translated by moving along the slide a (43); the fourth lens (52) is arranged on a sliding rail B (53), and axial translation is realized by moving along the sliding rail B (53); the sixth lens (62) is arranged on a slide rail C (63) and is axially translated by moving along the slide rail C (63).
3. The microscopic imaging apparatus with continuously adjustable magnification according to claim 1 or 2, characterized in that the imaging area is further provided with a three-dimensional displacement stage for moving the camera (7) in three dimensions.
4. The continuously variable magnification microscopic imaging apparatus according to claim 1 or 2, wherein the first lens group (41) is a single convex-concave lens with a convex surface facing the object side and a concave surface facing the image side.
5. The continuously variable magnification microscopic imaging apparatus according to claim 1 or 2, wherein the first lens group (41) comprises two lenses, in order from an object side to an image side: convex-concave lenses with convex surfaces facing the object side and concave surfaces facing the image side, and biconvex lenses with two convex surfaces of different curvatures.
6. The continuously variable magnification microscopic imaging apparatus according to claim 1 or 2, wherein the fifth lens group (61) is a single plano-convex lens with a convex surface facing the object side.
7. The continuously variable magnification microscopic imaging apparatus according to claim 1 or 2, wherein the fifth lens group (61) comprises two lenses, in order from the object side to the image side: a plano-convex lens with the convex surface facing the object side and a biconvex lens with two convex surfaces of different curvatures.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810555920.8A CN108828759B (en) | 2018-06-01 | 2018-06-01 | Microscopic imaging device with continuously adjustable magnification |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810555920.8A CN108828759B (en) | 2018-06-01 | 2018-06-01 | Microscopic imaging device with continuously adjustable magnification |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108828759A CN108828759A (en) | 2018-11-16 |
| CN108828759B true CN108828759B (en) | 2020-08-25 |
Family
ID=64147043
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810555920.8A Active CN108828759B (en) | 2018-06-01 | 2018-06-01 | Microscopic imaging device with continuously adjustable magnification |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108828759B (en) |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2744348B2 (en) * | 1990-11-20 | 1998-04-28 | キヤノン株式会社 | High zoom lens |
| US8254021B2 (en) * | 2009-06-23 | 2012-08-28 | Raytheon Canada Limited | Method and apparatus for specimen illumination in a microscope |
| JP6427344B2 (en) * | 2014-06-20 | 2018-11-21 | オリンパス株式会社 | Variable magnification relay optical system and imaging device |
| CN204422855U (en) * | 2015-01-29 | 2015-06-24 | 济南和普威视光电技术有限公司 | A kind of continuous vari-focus non-brake method focal length imaging lens |
| CN106842531A (en) * | 2015-12-04 | 2017-06-13 | 宁波华光精密仪器有限公司 | Can continuous zoom micro objective |
| CN206710069U (en) * | 2017-05-12 | 2017-12-05 | 南京邮电大学 | A kind of focal length of lens test device of no mechanical movement |
-
2018
- 2018-06-01 CN CN201810555920.8A patent/CN108828759B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN108828759A (en) | 2018-11-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US9772481B2 (en) | Arrangement for use in the illumination of a specimen in SPIM microscopy | |
| JP6996048B2 (en) | Wide field high resolution microscope | |
| US20140043469A1 (en) | Chromatic sensor and method | |
| CN106574899B (en) | Method and apparatus for microscopic examination of a sample | |
| DE102012109577A1 (en) | Arrangement for illuminating sample in selective plane illumination microscope, has light source for generating light beam and unit for generating light strip from light beam, particularly for planar-like illumination of sample | |
| WO2007011818A1 (en) | Microscope illumination device and adapter for dark- and bright-field illumination | |
| US10288860B2 (en) | Method and device for analysing an object, in particular a microscopic sample | |
| WO2014023344A1 (en) | Improved chromatic sensor and method | |
| CN108037310A (en) | A kind of image collecting device and acquisition method for microscopic particles imaging velocity-measuring system | |
| CN107976794A (en) | A kind of variable mating plate thickness and the mating plate of length illuminate microscopical lighting system | |
| EP1882970A1 (en) | Laser scanning microscope for fluorescence analysis | |
| CN109061865A (en) | It is a kind of can auto-focusing more multiplying power microscopic structures | |
| US12019005B2 (en) | Cell observation system and cell observation method | |
| CN107260120A (en) | A kind of camera and the common light path imaging devices of OCT | |
| CN105849615B (en) | Microscope for fadout illumination and dotted scanning illumination | |
| US10495865B2 (en) | Method and device for the SPIM analysis of a sample | |
| CN108828759B (en) | Microscopic imaging device with continuously adjustable magnification | |
| EP2290423B1 (en) | Scanning-microscope for optical scanning of samples with a diffusion plate in front of a transmitted light detector | |
| US20230074006A1 (en) | Apparatus and method for light field microscopy | |
| CN110554491B (en) | Miniature phase difference digital microscope for observing unstained cells of living body | |
| EP3074807B1 (en) | Optical arrangement for imaging a sample | |
| JP2022544292A (en) | Sample imaging via two-step brightfield reconstruction | |
| CN1779436A (en) | Coaxial-illuminating microscope otpical system for atomic force microscope | |
| CN117233947B (en) | Microscope illumination system, control method and microscopic imaging detection system | |
| RU2820220C1 (en) | Varifocal lens with telecentric beam path in spaces of objects and images |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |