CN108828759B - Microscopic imaging device with continuously adjustable magnification - Google Patents

Abstract

The invention relates to the field of microscopic imaging, and provides a microscopic imaging device with continuously adjustable magnification. The device comprises a coaxial light source, a sample area, a front module, a relay module, a rear module and an imaging area which are sequentially arranged; the relay module and the rear module can adjust the amplification factor of the whole device by adjusting the relative position of the movable lens. The experimental device has an open sample space, and can effectively control the visual field range and acquire experimental data with different resolutions by continuously adjusting the integral magnification.

Description

A microscope imaging device with continuously adjustable magnification

technical field

The invention relates to a microscopic imaging device, in particular to a microscopic imaging device with continuously adjustable magnification, belonging to the field of microscopic imaging.

Background technique

At present, microscopic imaging has become the main means of sample observation, and its advantage is that it can observe micron-scale samples and introduce light sources for optical testing. Traditional optical microscopes need to replace the objective lens to achieve intermittent adjustment of magnification, and the observation space is small, which is suitable for observing small samples at close range, but cannot observe large objects.

SUMMARY OF THE INVENTION

In view of this, the present invention provides a microscopic imaging device with continuously adjustable magnification, which has the advantages of flexible structure, large observation area space, continuously adjustable magnification range, adjustable field of view, simple and fast operation process and the like.

The microscopic imaging device with continuously adjustable magnification includes: a coaxial light source, an imaging area, a sample area, a front module, a relay module and a rear module sequentially arranged between the coaxial light source and the imaging area;

The coaxial light source is used to provide uniform illumination;

The sample to be imaged is arranged in the sample area in front of the coaxial light source;

The front module includes: a first lens group and a second lens that are coaxially arranged in sequence from the object side to the image side; the first lens group includes more than one lens, the whole of which is equivalent to a convex surface facing the object side and a concave surface facing the object side. A convex-concave lens on the image side; the second lens is a concave-convex lens with the concave surface facing the object side and the convex surface facing the image side; the position of the first lens group is fixed, and the second lens can translate along its axial direction;

The relay module includes: a third lens and a fourth lens coaxially arranged in sequence from the object side to the image side; wherein the third lens is a double concave lens; the fourth lens is a plano-convex lens with a convex surface facing the image side; the third lens The position of the lens is fixed, and the fourth lens can translate along its axis;

The rear module includes: a fifth lens group and a sixth lens coaxially arranged in sequence from the object side to the image side; the fifth lens group includes more than one lens, the whole of which is equivalent to a plano-convex lens with a convex surface facing the object side , the sixth lens is a concave-convex lens with the concave surface facing the object side and the convex surface facing the image side; the position of the fifth lens group is fixed, and the sixth lens can be translated along its axial direction;

The imaging area is photographed by a camera for the sample to be tested.

beneficial effect

(1) The microscopic imaging device proposed by the present invention can adjust the magnification (achieved by moving the second lens group and the fourth lens) according to the size of the experimental sample, and adjust the observation area; and realize the continuous magnification of the imaging device by adjusting the position of the lens Magnification adjustment, adjust the scope of the experimental field of view, the operation is simple, fast and flexible.

(2) The device proposed in the present invention has an open observation space, and can realize microscopic observation of large samples, such as combustion morphology in a container, flow state in an injector nozzle, nanowires, etc.

Description of drawings

1 is a schematic diagram of a microscope imaging device with continuously adjustable magnification according to the present invention;

In FIG. 2 , A is a schematic diagram of the internal flow of the transparent nozzle to be observed under low magnification, and B is a schematic diagram of the internal flow of the transparent nozzle to be observed under high magnification.

Among them: 1-light source, 2-fuel injector, 3-nozzle, 4-front module, 41-first lens group, 42-second lens, 43-slide rail A, 5-relay module, 51- The third lens, 52-fourth lens, 53-rail B, 6-rear module, 61-fifth lens group, 62-sixth lens, 63-rail C, 7-camera

Detailed ways

The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings. The embodiments listed here are only one of the embodiments, and do not represent all the embodiments.

The present embodiment provides a microscopic imaging device with continuously adjustable magnification. As shown in FIG. 1 , the microscopic imaging device includes: a coaxial light source 1 , an imaging area, and a coaxial light source 1 and an imaging area sequentially arranged between the coaxial light source 1 and the imaging area The sample area, front module 4, relay module 5 and rear module 6.

The coaxial light source 1 is used as a backlight light source to provide uniform illumination for the device.

A sample area is set in front of the coaxial light source 1 to provide experimental objects. In this embodiment, a fuel injector 2 is set in the sample area, the fuel injector tip nozzle 3 is made of plexiglass, and the sample to be imaged is the fuel injector tip nozzle 3 From the oil beam within the initial 0-2 cm and the fluid in the transparent nozzle, the development of the oil beam within the initial 0-2 cm of the nozzle 3, the flow state change in the transparent nozzle and the combustion of the oil beam can be obtained.

The front module 4 is used to gather the light beams emitted by the coaxial light source 1. The front module 4 can achieve different focal lengths and numerical apertures through different lens combinations. The front module 4 includes: a front lens module and a slide rail A43. In the lens module 4, from the object side (the side where the sample area is located) to the image side (the side where the imaging area is located), there are a first lens group 41 and a second lens 42 arranged coaxially in sequence. The first lens group 41 includes more than one lens, the whole of which is equivalent to a convex-concave lens with a convex surface facing the object side and a concave surface facing the image side (for example, it can be a single convex-concave lens with a convex surface facing the object side, a concave surface facing the image side, or a single convex surface facing the image side A lens group consisting of a convex-concave lens with a concave surface facing the image side on the object side and a biconvex lens with two convex surfaces with different curvatures); the second lens 42 is a concave-convex lens with the concave surface facing the object side and the convex surface facing the image side. The position of the first lens group 41 is fixed, and the second lens 42 is connected to the slide rail A43, and can slide along the slide rail A43 to change the relative position with the first lens 41. In this embodiment, the focal length range of the front module 4 is 30mm ~50mm.

The relay module 5 includes: a relay lens module and a sliding rail B53; the relay lens module is, from the object side to the image side, a third lens 51 and a fourth lens 52 arranged coaxially in sequence. The third lens 51 is a double concave lens; the fourth lens 52 is a plano-convex lens with a convex surface facing the image side; the position of the third lens 51 is fixed; The relative positions of the three lenses 51 .

The rear-mounted module 6 includes a rear-mounted lens module and a slide rail C63; wherein the rear-mounted lens module is, from the object side to the image side, a fifth lens group 61 and a sixth lens 62 arranged coaxially in sequence. The fifth lens group 61 includes more than one lens, the whole of which is equivalent to a plano-convex lens with a convex surface facing the object side (for example, it can be a plano-convex lens with a single convex surface facing the object side or a plano-convex lens with one convex surface facing the object side and one with two convex surfaces facing the object side). The sixth lens 62 is a meniscus lens with the concave surface facing the object side and the convex surface facing the image side. The position of the fifth lens group 61 is fixed; the sixth lens 62 is connected to the sliding rail C63 and can slide along the sliding rail C63 to change the relative position with the fifth lens group 61 .

The imaging area is provided with a high-speed camera 7, a three-dimensional displacement stage and a control unit for controlling the three-dimensional displacement stage; the high-speed camera 7 is arranged on the three-dimensional displacement stage for controlling the displacement of the high-speed camera 7 in the X, Y, and Z directions (wherein The axial direction of each lens is the X direction, the vertical direction is the Z direction, and the direction perpendicular to the XZ plane is the Y direction).

The working principle of the microscopic imaging device is as follows: the coaxial light source 1 illuminates the transparent nozzle 3, and the nozzle 3 is made of organic glass with a light transmittance as high as 92%, and the light loss is negligible. Due to the refraction and scattering of light, the air bubbles in the nozzle 3 block the light, which appears dark on the image of the high-speed camera 7, and the liquid oil part appears bright on the camera.

The light beam emitted by the light source 1 is converged by the first lens group 41 after passing through the sample. The converged light beam passes through the focal point of the first lens group 41 and becomes a diverging beam, and is then converged by the second lens 42. The beam divergence angle decreases, but is still Diverging the light beam, and then exit to the relay module 5 . The front module 4 can achieve different focal lengths and numerical apertures through different lens combinations. In this embodiment, the lens group 4 achieves a focal length of 30-50 mm.

The divergent light emitted from the front module 4 is diverged by the third lens 51 in the relay module 5 , and the central part of the divergent light beam is converged by the fourth lens 52 and then exits the rear module 6 .

The light beam emitted from the relay module 5 is condensed by the fifth lens group 61 in the rear module 6 , and the condensed light beam is slightly diverged by the sixth lens 62 to extend the focal plane position. The equivalent focal length of the fifth lens group 61 and the sixth lens 62 in the rear module 6 is 180 mm, and the numerical aperture is f 3.5.

When the second lens 42 in the front module 4 is moved backward, the central beam enters the third lens 51, the edge portion cannot be incident on the third lens, and the magnification increases; otherwise, the magnification decreases when it moves forward; the third lens 51 exits The light beam is incident on the fourth lens 52. When the fourth lens 52 moves forward, the central beam incident on the fifth lens group 61 increases, and the magnification decreases; on the contrary, when the fourth lens 52 moves backward, the magnification increases; the position of the focal plane increases with the magnification. When the magnification is changed, by moving the sixth lens 62, the position of the focal plane can be changed, and the focal plane can be moved to the position where the camera chip is located.

In the above device, all movable lenses have a unique degree of freedom (axial movement).

In the above device, the high-speed camera 7 is used for imaging to obtain high time resolution data of the sample. The high-speed acquisition and short exposure time result in a reduction in the number of photons captured by the camera, resulting in a darker image. The front module, the relay module and the rear module all use high transmittance lenses to reduce light loss and increase luminous flux. The microscopic imaging device can obtain experimental data and images with more details, and can be applied to observe sample morphology, fuel oil development, cavitation formation, etc. The magnification of the imaging device is adjustable, and the adjustment range is 5-20 times.

Although the embodiments of the present invention are described in conjunction with the accompanying drawings, for those skilled in the art, without departing from the principles of the present invention, several modifications and improvements can be made, which should also be regarded as belonging to the present invention. protected range.

Claims (7)

1. A microscopic imaging apparatus with continuously adjustable magnification, comprising: the device comprises a coaxial light source (1), an imaging area, a sample area, a front module (4), a relay module (5) and a rear module (6), wherein the sample area, the front module (4), the relay module (5) and the rear module are sequentially arranged between the coaxial light source (1) and the imaging area;

the coaxial light source (1) is used for providing uniform illumination;

the sample to be imaged is arranged in a sample area in front of the coaxial light source (1);

the front module (4) comprises: a first lens group (41) and a second lens (42) coaxially arranged in order from an object side to an image side; the first lens group (41) comprises more than one lens, and the whole lens is equivalent to a convex-concave lens with a convex surface facing to the object side and a concave surface facing to the image side; the second lens (42) is a concave-convex lens with a concave surface facing the object side and a convex surface facing the image side; the first lens group (41) is fixed in position, and the second lens (42) can translate along the axial direction thereof;

the relay module (5) comprises: a third lens (51) and a fourth lens (52) coaxially disposed in order from the object side to the image side; wherein the third lens (51) is a biconcave lens; the fourth lens (52) is a plano-convex lens with a convex surface facing the image side; the third lens (51) is fixed in position, and the fourth lens (52) can translate along the axial direction of the third lens;

the rear module (6) comprises: a fifth lens group (61) and a sixth lens (62) coaxially arranged in order from the object side to the image side; the fifth lens group (61) comprises more than one lens, the whole lens is equivalent to a plano-convex lens with a convex surface facing to the object side, and the sixth lens (62) is a concave-convex lens with a concave surface facing to the object side and a convex surface facing to the image side; the fifth lens group (61) is fixed in position, and the sixth lens (62) can translate along the axial direction of the fifth lens group;

the imaging area photographs the sample to be measured through a camera (7).

2. The continuously variable magnification microscopic imaging apparatus according to claim 1, wherein the second lens group (42) is disposed on a slide a (43) and is axially translated by moving along the slide a (43); the fourth lens (52) is arranged on a sliding rail B (53), and axial translation is realized by moving along the sliding rail B (53); the sixth lens (62) is arranged on a slide rail C (63) and is axially translated by moving along the slide rail C (63).

3. The microscopic imaging apparatus with continuously adjustable magnification according to claim 1 or 2, characterized in that the imaging area is further provided with a three-dimensional displacement stage for moving the camera (7) in three dimensions.

4. The continuously variable magnification microscopic imaging apparatus according to claim 1 or 2, wherein the first lens group (41) is a single convex-concave lens with a convex surface facing the object side and a concave surface facing the image side.

5. The continuously variable magnification microscopic imaging apparatus according to claim 1 or 2, wherein the first lens group (41) comprises two lenses, in order from an object side to an image side: convex-concave lenses with convex surfaces facing the object side and concave surfaces facing the image side, and biconvex lenses with two convex surfaces of different curvatures.

6. The continuously variable magnification microscopic imaging apparatus according to claim 1 or 2, wherein the fifth lens group (61) is a single plano-convex lens with a convex surface facing the object side.

7. The continuously variable magnification microscopic imaging apparatus according to claim 1 or 2, wherein the fifth lens group (61) comprises two lenses, in order from the object side to the image side: a plano-convex lens with the convex surface facing the object side and a biconvex lens with two convex surfaces of different curvatures.

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