CN108828051A - The lipid real-time detection method of the antarctic krill oil of rapid evaporation ionization massspectrum - Google Patents
The lipid real-time detection method of the antarctic krill oil of rapid evaporation ionization massspectrum Download PDFInfo
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Abstract
The invention discloses the lipid real-time detection methods of the antarctic krill oil of rapid evaporation ionization massspectrum.It the described method comprises the following steps：A, antarctic krill oil is extracted with ethyl alcohol；B, mass spectrograph detection is introduced after ionizing antarctic krill oil, obtains mass spectrum profile diagram；C, mass spectrum profile diagram is determined to the iipidomic profile of sample after MassLynx is compared.The present invention need not carry out sample pre-treatments, real-time Mass Spectrometer Method, the iipidomic detection suitable for food can be achieved, and can promote the shrimp sauce that krill is raw material and develop.
Description
Technical field
The present invention relates to the detection method of the lipid of antarctic krill oil, especially a kind of rapid evaporation ionization massspectrum
The lipid real-time detection method of antarctic krill oil.
Background technique
Iipidomic (Lipidomics) is the popular neck of the another rapid development after genomics and proteomics
Domain, research contents include biological body lipid molecular species Structural Identification and it is quantitative and its in biological metabolism, disease, exempt from
The analysis of institutes' role such as epidemic disease.With the development of soft ionization ionization techniques and high resolution mass spectrum technology and its in lipid point
Application in analysis has been realized in and carries out quick, high-precision analysis detection to micro lipids various in biological sample, greatly
Scale, the lipid analysis of globality must be promoted.In recent years mass-spectrometric technique using more and more extensive, researcher couple
Higher requirements are also raised for mass spectrum high-flux quick detection technique.However biological organization sample cannot be used directly for it is highly sensitive
The Mass Spectrometer Method of degree, it usually needs professional is through complicated cumbersome samples such as tissue homogenate, the purifying of compound extracting and developing
After pre-treatment step, it can be used for mass spectral analysis, therefore cannot achieve the real-time Mass Spectrometer Method research of tissue samples, can not also be expired
The demand of foot high throughput omics technology research.The extracting method that iipidomic detection routinely uses is Folch method and Bligh&
Dyer method, however both methods needs to use the higher organic reagent of the toxicity levels such as chloroform, is not suitable for the lipid of food
Group learns detection.
Rapid evaporation ionization massspectrum (Rapid evaporative ionization mass spectr ometry,
REIMS it is) a kind of emerging technological means for the identification of clinical biochemical tissue in situ dynamic realtime, is burnt with thermoelectricity and lose biology
Based on suck tissue, induce biomolecular ions, formed the aerosol containing charged particle, through ion transfer tube road into
Enter spectrometer analysis.200 9 years, Takatas et al. was in desorption electrospray technology (Desorption electrospray
Ioniza tion, DESI) on the basis of couple high-resolution mass spectrometer based on high-frequency electric knife device and have developed R EIMS, be in situ
Ionization mass spectrometry research and mass spectrum metabolism group research provide a kind of completely new research method and Research Thinking.
Krill (Euphausia superba) is a kind of shell-fish zooplankter lived in Southern Oceans, tool
There is the features such as biological reserves are big, distribution is wide, is important strategic ocean new resources.It is estimated that krill storage has
Hundred million tons of 6.5-10, maximum tolerance amount of fishing is about ten thousand tons of 400-600.The back that marine fishery resources is increasingly decayed in the world
Under scape, the country such as Japan, Norway is sequentially added the research and development of krill, and the Related products industry such as existing krill oil
Change.It is rich in phosphatide mating type n-3 fatty acid in krill, is easier to absorb than the triglyceride type n-3 fatty acid in fish oil,
It is effective source of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA).Experiment shows that antarctic krill oil has drop
Low cholesterol, prevention senile dementia, prevention of arterial hardening, anti-inflammatory, protection eyesight and improvement brain learning function etc. are raw
Manage function.For the research of krill iipidomic, there is not been reported at present, and carrying out the research of krill iipidomic will deepen
Krill lipid nutrition understanding promotes the shrimp sauce that krill is raw material to develop.
Therefore, existing iipidomic profile testing method, that there are sample pre-treatments is cumbersome, cannot achieve real-time mass spectrum
The problem of detecting, not being suitable for the iipidomic detection of food；Still for the iipidomic contour detecting of antarctic krill oil at present
It has not been reported.
Summary of the invention
The purpose of the present invention is to provide a kind of lipid real-time detection of the antarctic krill oil of rapid evaporation ionization massspectrum
Method.The present invention need not carry out sample pre-treatments, real-time Mass Spectrometer Method, the iipidomic detection suitable for food can be achieved.
Technical solution of the present invention：The lipid real-time detection method of the antarctic krill oil of rapid evaporation ionization massspectrum, packet
Include following steps：
A, the extraction of antarctic krill oil：It is freeze-dried after krill is homogenized, obtains A1 product, weighed A1 product and be placed in centrifugation
Ethyl alcohol is added to centrifuge tube in Guan Zhong, and concussion mixes, and room temperature extracts in thermostat water bath, is centrifuged with refrigerated centrifuge, shifts
Supernatant is added ethyl alcohol to lower sediment and repeats to be extracted twice, merges supernatant, be evaporated ethyl alcohol at 65 DEG C to obtain grease
Concentrate, i.e. A2 product；
B, A2 product are ionized to form aerosol by heated probe, the temperature of the heated probe is 500 DEG C, institute's shape
At aerosol pumped by Venturi and driven through nitrogen with the pressure of 2bar, mass spectrograph is introduced through PTFE tube using orthogonal manner,
Using propylene glycol/leucine enkephalin mixture as secondary solvent, sample injection device inlet port is pumped through needle；The mass spectrometer interface
It inside sets 1. 1 Ω, 3V, 500 DEG C of Kanthal A1 filament to ionize as secondary impingement, enter after ion collision mass spectrometric
StepWave device；The mass spectrometric sweep time is 1 second, and scanning range is m/z 50-1200；It is received with negative electricity from mode
Collect data, obtains antarctic krill oil mass spectrum profile diagram；
C, mass spectrum profile diagram is subjected to peak match through MassLynx, peak alignment, filters processing of making an uproar, as a result save as text lattice
Formula obtains the mass-to-charge ratio for the mass spectra peak that relative amount is 5% or more according to mass spectrometric data, the master that relative amount is 10% or more
Peak fragment ion information determines the iipidomic profile of sample after comparing in conjunction with given data.
In the lipid real-time detection method of the antarctic krill oil of rapid evaporation ionization massspectrum above-mentioned, in step A, institute
State the ethyl alcohol that ethyl alcohol is 95%；It is described that ethyl alcohol is added to centrifuge tube, it is that the amount of 5ml ethyl alcohol is added to be added with every gram of sample；It is described from
Scheming is centrifuged 10m in 9000r/min；It is described that ethyl alcohol is added to lower sediment, it is that every gram of sample adds the amount of 3ml ethyl alcohol to be added；
The extraction time is 2h.
In the lipid real-time detection method of the antarctic krill oil of rapid evaporation ionization massspectrum above-mentioned, in step A, institute
The mass ratio for stating propylene glycol and leucine enkephalin is 98:2.
In the lipid real-time detection method of the antarctic krill oil of rapid evaporation ionization massspectrum above-mentioned, in step B, institute
The flow stated in needle pump sample injection device is 0.1mL/min.
In the lipid real-time detection method of the antarctic krill oil of rapid evaporation ionization massspectrum above-mentioned, in step B, institute
State and ionize A2 product by heated probe, be pass through after the soybean oil of the preparatory degumming of A2 product is diluted 4 times heated probe from
Sonization.
Compared with the prior art, the present invention uses the iipidomic wheel of REIMS high throughput real-time detection antarctic krill oil
Exterior feature, to realize the real-time detection of lipid.Negative electricity from antarctic krill oil under mode by electric heating pop one's head in or single-stage electrode ionization,
Aerosol is formed, aerosol is formed by and is driven by pumping through nitrogen with the pressure of 2bar, introduced using orthogonal manner through PTFE tube
Mass spectrometer parameters are arranged in mass spectrograph, and mass spectrograph, which only scans 1 second, can be obtained mass spectrum profile diagram, and being compared by software can afterwards really
The iipidomic profile of random sample product.Quickly, efficiently, related data can be mass spectrum work to this method iipidomic profile real-time detection
Author provides fundamental basis, and provides related foundation and reference to department for functional sea food enterprise.Antarctic krill oil is through quality
Analyzer detection detects 20 kinds of fatty acid and 26 kinds of phospholipid molecules altogether, wherein EPA (m/z 301,21.59%) and D HA (m/z
327,14.44%) it is leading ion peak in mass spectrogram.The present invention need not carry out sample pre-treatments, real-time mass spectrum inspection can be achieved
It surveys, suitable for the iipidomic detection of food, can promote the shrimp sauce that krill is raw material and develop.
Detailed description of the invention
Fig. 1 is the detection device of REIMS high throughput real-time detection antarctic krill oil iipidomic profile；
Fig. 2 is influence of the concentration of alcohol to krill oil extract yield；
Fig. 3 is antarctic krill oil REIMS mass spectrum profile diagram；
Fig. 4 is fatty acid ion peak in the region antarctic krill oil iipidomic profile diagram m/z 200-500；
Fig. 5 is the phosphatide quasi-molecular ions within the scope of antarctic krill oil iipidomic profile diagram m/z 400-900.
Appended drawing reference in figure is：1- controller, 2- electric heating probe, 3- antarctic krill oil concentrate, 4- aerosol, 5-
Syringe pump (i.e. needle pumps sample injection device), 6- mass spectrograph.
Specific embodiment
The present invention will be further described below with reference to the drawings, but is not intended as the foundation limited the present invention.
It is experimental example of the invention below.
Experimental example.
1.1 key instruments and device
Xevo G2-XS quadrupole rod time of-flight mass spectrometer：Waters, US's product is furnished with rapid evaporation ion
Source；Pump11elite needle pumps sample injection device：U.S.'s Harvard Products；2150 ultrapure water processing system of Milliplus
System：U.S.'s Millipore Products；4.1 data acquisition software of Masslynx and LiveID statistical analysis software：U.S. W
Aters Products；Other is laboratory common instrument and equipment.
1.2 main materials and reagent
Krill：Distant fishing krill development in science and technology Co., Ltd product；Methanol, acetonitrile, isopropanol：It is chromatography
It is pure, German Meker Products；Formic acid：Chromatographically pure, U.S.'s T edia Products；Leucine enkephalin：U.S. Sigma-
Aldrich product；Other laboratory common agents are that analysis is pure.
1.3 experimental condition
1.3.1 antarctic krill oil extraction conditions will be freeze-dried after the homogenate of krill tissue, accurate weighing 5.0g sample
In 50mL centrifuge tube, mixing is shaken after 95% ethyl alcohol of 25mL is added, room temperature extracts 2h in thermostat water bath；Mixture is used
Refrigerated centrifuge is centrifuged 10min with 9000 r/min, shifts supernatant with pipette, and 9 5% second of 15mL is added to lower sediment
Alcohol repeats to be extracted twice, and merges supernatant；It at 65 DEG C is evaporated the organic solvent to obtain antarctic krill oil with Rotary Evaporators dense
Contracting liquid.
1.3.2 Mass Spectrometry Conditions krill oil samples are formed by aerosol by (500 DEG C) of heated probe ionizations
By Venturi pump nitrogen (2bar) driving, mass spectrum, main device such as Fig. 1 are introduced through PTFE tube using orthogonal manner；With the third two
(mass ratio of propylene glycol and leucine enkephalin is 98 to alcohol/leucine enkephalin mixture:2) it is secondary solvent, is pumped through needle
Sample injection device is with the flow inlet port of 0.1mL/min, for cleaning impurity, improving signal strength, lock mass correction；
Kanthal A1 filament (1.1 Ω, 3V, 500 DEG C) is set in mass spectrometer interface to ionize as secondary impingement, and matter is entered after ion collision
Spectrometer StepWave device；Mass spectrograph sweep time：1 second；Scanning range：m/z 50-1200；All data with negative electricity from
Mode is collected.
1.4 statistical analysis
Raw mass spectrum figure is subjected to peak match through MassLynx, peak alignment, filters processing etc. of making an uproar, as a result saves as text lattice
Formula.The mass-to-charge ratio for the mass spectra peak that relative amount is 5% or more, the master that relative amount is 10% or more are obtained according to mass spectrometric data
Peak fragment ion information etc., in conjunction with the comparison of Lip id MS Predictor and LipidMap database search criteria product and document
Report determines or speculates the structural information of lipid.
2 results
The optimization of 2.1 krill oil extracts
The extracting method that iipidomics analysis routinely uses is Folch method and Bligh&Dyer method, however both methods
Need to use the higher organic reagent of the toxicity levels such as chloroform.Safety in view of antarctic krill oil as health food refers to
Mark, this experiment, as extraction media, extract antarctic krill oil from krill dry powder using ethyl alcohol.Compare and optimizes difference
Influence of the ethanol solution of concentration to antarctic krill oil recovery rate.As a result as shown in Fig. 2, the yield of antarctic krill oil is with second
Alcohol content increases and improves, wherein 95% ethyl alcohol extraction effect is best, when using straight alcohol, yield is declined.
2.2REIMS iipidomic edge analysis
REIMS is a kind of real-time mass spectrometry method without any sample pre-treatments, passes through the direct surface ion to original sample
Change, obtain mass spectrum profile diagram in real time, using the softwares contour identification figure such as LiveID and to characteristic ion peak dimensionality reduction, modeling after, can
The realtime qualification and accuracy for realizing unknown sample score.
The common ion source of REIMS makes the small molecule on surface by cutting tissue at a given current for monopolar electrode
Rapid evaporation ionization, and mass spectrum port is entered by ion transfer tube road.It, can not since antarctic krill oil is liquid sample
Using monopolar electrode cut mode, therefore this experiment makes antarctic krill oil be heated rapid evaporation by the way of electric heating probe, lipid
Compound ions simultaneously enter mass spectrum mass analyzer with aerosol together.Krill oil meter is touched using electric heating probe
Face, scanning of the mass spectrum range are set in m/z 0-1200, record mass spectrometric data by MassLynx.As shown in figure 3, krill
Lipid molecular quasi-molecular ions mainly appears on 200-900 regional scope of m/z, and is made of two apparent peak clusters, i.e. m/z
The fatty acid ion peak cluster of 200-500 and the phosphatide quasi-molecular ions cluster of m/z 600-900.From the point of view of relative amount, fatty acid from
The signal response at sub- peak is significantly stronger than the signal strength of phosphatide quasi-molecular ions, the former peak area is about 7.6 times of the latter.Fat
Source a part of acid ion is that the free fatty acid ionization in antarctic krill oil generates, and another part is by glyceride, phosphorus
The lipids such as rouge, cholesteryl ester are reflected in mass spectrogram after the ionization of fatty acid chain fragmentation by electric heating probe high energy cracking.
Fig. 4 is fatty acid ion peak in the region antarctic krill oil iipidomic profile diagram m/z 200-500, each peak point
Preferably from degree, noise is relatively high to be hardly visible matrix peak；By identifying it and integrating, obtains structure and relative amount is shown in
Table 1.Maximum signal response intensity is m/z 301, and relative amount has reached 21.59%, its identified structure is FA 20:5,
That is known EPA.It secondly is respectively the (FA 22 of m/z 327:6/DHA, 14.44%), (FA 18 of m/z 281:1,
And (the FA 16 of m/z 255 12.62%):0,18.74%).Doubtful FA 18 is detected in antarctic krill oil:4 fatty acid from
Sub- peak m/z 275 (3.78%), the component usually detect in vegetalitas oil crops.Meanwhile m/z 391, m/z 417 etc.
Doubtful is over-long chain fatty acid FA 26:2 (6.17%), FA 28:3 (2.10%), existing may be with krill by pole
Cold environment-stress promotes its metabolism to generate long chain fatty acids related to improve body fluid mobility；Correlation assume it is still necessary to biology into
One step card.
Fatty acid relative amount and structural analysis in 1. antarctic krill oil of table
Note：1) fatty acid structure is to be parsed according to experience and software as a result, not yet carrying out structure verification；Representation
For FA c:D, wherein c is the carbon atom number of fatty acid chain, and d is double key number.
Fig. 5 is the phosphatide quasi-molecular ions within the scope of m/z 400-900, since the signal response of phosphatide is substantially less than fatty acid,
Matrix effect is relatively strong, after noise reduction and peak extract, identifies 26 kinds of phospholipid molecules altogether.Wherein the strongest quasi-molecular ions of signal is
M/z 763 (11.67%), doubtful structure is [PG 36:7-H]-；Followed by m/z 737 (11.46%), structure [PC o-
38: 3-choline]-/[PE o-38:3-NH3]-.Identical c:Under d ratio, PC loses the molecular structure and PE after choline fragment
Deamination based structures are identical, therefore cannot be distinguished in REIMS iipidomic Profile Spectrum.By taking mass spectra peak m/z 695.4 as an example, warp
It identifies the entire carbon atom of its sn1/sn2 fatty acid chain and double-strand quantity is 34:3, molecular structure composition may be [PC-
choline] –, or [PE-NH3]–, also or the overlapping of the two.PI molecule is less in antarctic krill oil, only m/z 835
([PI 34:1-H]-, 1.79%) and ([the PI o-36 of m/z 843:4-H]-, 1.47%) and detection.
Phosphatide relative amount and structural analysis in 2. antarctic krill oil of table
2.3 methodology validation
Organic solvent burning point is lower, open fire easily occurs by electric heating probe ionization, there has been no building standard quantitatives at present
Dicyandiamide solution.Therefore, the sensitivity test in methodology validation uses multiple dilution method, by adding into antarctic krill oil
The soybean oil of preparatory degumming carries out REIMS detection after being diluted.The results show that 26 kinds of phosphatide after 4 times of antarctic krill oil dilution
Molecule still can Rapid Detection, spectrogram is close with Fig. 5 after noise reduction, except signal strength reduce in addition to iipidomic contour feature
Similar, there were significant differences；The phospholipid molecule number that detects is reduced to 14 kinds after antarctic krill oil dilutes 10 times, each phosphatide from
The signal strength equal proportion at sub- peak declines, partial contour Character losing.Accuracy test is accurate using withinday precision and in the daytime
Degree evaluation,
Fatty acid and phosphatide respectively choose two ion m/z 301, m/z 327, m/z 763 and m/z 737, respectively at same
METHOD FOR CONTINUOUS DETERMINATION on the one 7 times and METHOD FOR CONTINUOUS DETERMINATION 5 days, it is calculated its withinday precision RSD≤6.24%, withinday precision RSD≤
8.57%.Methodology validation is the results show that this method is able to satisfy the iipidomic profile of fatty acid and phosphatide in antarctic krill oil
Analyze test request.
3 conclusions
Real-time iipidomic profile to the lipid direct ion in antarctic krill oil and is obtained using REIMS technology.?
Antarctic krill oil is excited into ionic state by electric heating probe under negative ion mode, and introduces mass spectrum port with aerosol, through quality point
Parser detection shares 20 kinds of fatty acid and 26 kinds of phospholipid molecule detections, wherein EPA (m/z 301,21.59%) and DHA (m/z
327,14.44%) it is leading ion peak in mass spectrogram.This method to antarctic krill oil iipidomic profile real-time detection quickly,
Efficiently, related data can provide fundamental basis for mass spectrum worker, provide related foundation to department for functional sea food enterprise
And reference.
It is the embodiment of the present invention below.
Embodiment.
The lipid real-time detection method of the antarctic krill oil of rapid evaporation ionization massspectrum, includes the following steps：
A, the extraction of antarctic krill oil：It is freeze-dried after krill is homogenized, obtains A1 product, weighed A1 product and be placed in centrifugation
Ethyl alcohol is added to centrifuge tube in Guan Zhong, and concussion mixes, and room temperature extracts in thermostat water bath, is centrifuged with refrigerated centrifuge, shifts
Supernatant is added ethyl alcohol to lower sediment and repeats to be extracted twice, merges supernatant, be evaporated ethyl alcohol at 65 DEG C to obtain grease
Concentrate, i.e. A2 product；
B, A2 product are ionized to form aerosol by heated probe, the temperature of the heated probe is 500 DEG C, institute's shape
At aerosol pumped by Venturi and driven through nitrogen with the pressure of 2bar, mass spectrograph is introduced through PTFE tube using orthogonal manner,
Using propylene glycol/leucine enkephalin mixture as secondary solvent, sample injection device inlet port is pumped through needle；The mass spectrometer interface
It inside sets 1. 1 Ω, 3V, 500 DEG C of Kanthal A1 filament to ionize as secondary impingement, enter after ion collision mass spectrometric
StepWave device；The mass spectrometric sweep time is 1 second, and scanning range is m/z 50-1200；It is received with negative electricity from mode
Collect data, obtains antarctic krill oil mass spectrum profile diagram；
C, mass spectrum profile diagram is subjected to peak match through MassLynx, peak alignment, filters processing of making an uproar, as a result save as text lattice
Formula, the mass-to-charge ratio for the mass spectra peak for being 5% or more according to the relative amount that mass spectrometric data obtains, relative amount are 10% or more
Main peak fragment ion information determines the iipidomic profile of sample after comparing in conjunction with given data.
In step A, the ethyl alcohol be 95% ethyl alcohol；It is described that ethyl alcohol is added to centrifuge tube, it is that 5ml is added with every gram of sample
The amount of ethyl alcohol is added；The centrifuge is centrifuged 10m in 9000r/min；It is described that ethyl alcohol is added to lower sediment, it is every gram of sample
Product add the amount of 3ml ethyl alcohol to be added；The extraction time is 2h.
In step A, the mass ratio of the propylene glycol and leucine enkephalin is 98:2.
In step B, the flow in the needle pump sample injection device is 0.1mL/min.
It is described to ionize A2 product by heated probe in step B, it is that the soybean oil of the preparatory degumming of A2 product is diluted 4
It is ionized after times by heated probe.
Certainly, the present invention can also have other various embodiments, without deviating from the spirit and substance of the present invention,
Those skilled in the art make various corresponding changes and modifications, but these corresponding changes in accordance with the present invention
It all should fall within the scope of protection of the appended claims of the present invention with deformation.
Claims (5)
1. the lipid real-time detection method of the antarctic krill oil of rapid evaporation ionization massspectrum, which is characterized in that including following step
Suddenly：
A, the extraction of antarctic krill oil：It is freeze-dried after krill is homogenized, obtains A1 product, weighed A1 product and be placed in centrifuge tube,
Ethyl alcohol is added to centrifuge tube, concussion mixes, and room temperature extracts in thermostat water bath, is centrifuged with refrigerated centrifuge, supernatant is shifted,
Ethyl alcohol is added to lower sediment to repeat to be extracted twice, merges supernatant, is evaporated ethyl alcohol at 65 DEG C to obtain grease concentrate, i.e.,
A2 product；
B, A2 product are ionized to form aerosol by heated probe, the temperature of the heated probe is 500 DEG C, is formed by gas
Colloidal sol is pumped by Venturi and is driven through nitrogen with the pressure of 2bar, introduces mass spectrograph through PTFE tube using orthogonal manner, with the third two
Alcohol/leucine enkephalin mixture is secondary solvent, pumps sample injection device inlet port through needle；1.1 are set in the mass spectrometer interface
Ω, 3V, 500 DEG C of Kanthal A1 filament are ionized as secondary impingement, and mass spectrometric StepWave is entered after ion collision and is filled
It sets；The mass spectrometric sweep time is 1 second, and scanning range is m/z 50-1200；Data are collected from mode with negative electricity, are obtained
Antarctic krill oil mass spectrum profile diagram；
C, mass spectrum profile diagram is subjected to peak match through MassLynx, peak alignment, filters processing of making an uproar, as a result save as text formatting, root
The mass-to-charge ratio for the mass spectra peak that relative amount is 5% or more is obtained according to mass spectrometric data, the main peak fragment that relative amount is 10% or more
Ion information determines the iipidomic profile of sample after comparing in conjunction with given data.
2. the lipid real-time detection method of the antarctic krill oil of rapid evaporation ionization massspectrum according to claim 1,
It is characterized in that：In step A, the ethyl alcohol be 95% ethyl alcohol；It is described that ethyl alcohol is added to centrifuge tube, it is that 5ml is added with every gram of sample
The amount of ethyl alcohol is added；The centrifuge is centrifuged 10min with 9000r/min；It is described that ethyl alcohol is added to lower sediment, it is every gram of sample
The amount of 3ml ethyl alcohol is added to be added；The extraction time is 2h.
3. the lipid real-time detection method of the antarctic krill oil of rapid evaporation ionization massspectrum according to claim 1,
It is characterized in that：In step A, the mass ratio of the propylene glycol and leucine enkephalin is 98:2.
4. the lipid real-time detection method of the antarctic krill oil of rapid evaporation ionization massspectrum according to claim 1,
It is characterized in that：In step B, the flow in the needle pump sample injection device is 0.1mL/min.
5. the lipid real-time detection method of the antarctic krill oil of rapid evaporation ionization massspectrum according to claim 1,
It is characterized in that：It is described to ionize A2 product by heated probe in step B, it is that the soybean oil of the preparatory degumming of A2 product is diluted 4
It is ionized after times by heated probe.
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| CN110514732A (en) * | 2019-09-03 | 2019-11-29 | 北京出入境检验检疫局检验检疫技术中心 | Identify the method for the sea-buckthorn place of production and/or kind based on rapid evaporation ionization mass spectrometry technology |
| CN111812248A (en) * | 2020-07-23 | 2020-10-23 | 浙江工商大学 | Analysis and detection method for effectively screening phospholipids in krill oil |
| CN112782266A (en) * | 2021-02-10 | 2021-05-11 | 中国检验检疫科学研究院 | Method for identifying fresh meat products and frozen and thawed meat products |
| CN113720896A (en) * | 2021-09-26 | 2021-11-30 | 浙江工商大学 | In-situ rapid evaporation ionization mass spectrometry for real-time identification of seven shrimps |
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| CN112782266A (en) * | 2021-02-10 | 2021-05-11 | 中国检验检疫科学研究院 | Method for identifying fresh meat products and frozen and thawed meat products |
| CN113720896A (en) * | 2021-09-26 | 2021-11-30 | 浙江工商大学 | In-situ rapid evaporation ionization mass spectrometry for real-time identification of seven shrimps |
| CN113740410A (en) * | 2021-09-26 | 2021-12-03 | 浙江工商大学 | Method for detecting wet-type maturation maturity of tuna meat |
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