CN108828041A - A kind of building and its application of electrochemical sensor - Google Patents
A kind of building and its application of electrochemical sensor Download PDFInfo
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- CN108828041A CN108828041A CN201811089873.9A CN201811089873A CN108828041A CN 108828041 A CN108828041 A CN 108828041A CN 201811089873 A CN201811089873 A CN 201811089873A CN 108828041 A CN108828041 A CN 108828041A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/308—Electrodes, e.g. test electrodes; Half-cells at least partially made of carbon
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/48—Systems using polarography, i.e. measuring changes in current under a slowly-varying voltage
Abstract
It is to use molecular formula for C the invention discloses a kind of building of electrochemical sensor and its application45H60N6O6, reduced graphene composite material is made in structural formula macrocyclic compound functionalization reduced graphene as shown in Figure 1, with reduced graphene composite material processing glass-carbon electrode to get electrochemical sensor.Its application is the application in dopamine detection.Sensor of the invention has easy to operate, response quickly, low in cost, and accuracy is high, and high sensitivity, detection limit is low, and the characteristics of stability and favorable reproducibility.
Description
Technical field
The present invention relates to a kind of building of sensor and its application, the building of especially a kind of electrochemical sensor and its answer
With.
Background technique
Electrochemical sensor is a kind of special device that electroanalytical technique is combined with sensing technology.Its working principle is
The chemistry of determinand or biological information are converted to the response signal that conducting system can produce, such as electric current, current potential.By behaviour
Make simplicity, response quickly, low in cost, accuracy is high, high sensitivity, detection limit are low etc., and advantages, electrochemical sensor have developed
As the ideal analysis tool being most widely used.But there is also some defects for electrochemical sensor, such as vulnerable to electroactive
Interference, poor reproducibility and long-time stability difference of substance etc..Core component of the working electrode as electrochemical sensor, often
Decide the sensing capabilities of sensor.Therefore, novel electrode decorative material is developed, stability is improved and reproducibility is this always
The research topic in field.
Reduced graphene is a kind of two-dimentional carbon nanomaterial with monoatomic thickness, it have excellent electronics, optics,
Calorifics, chemistry and engineering properties, in addition, reduced graphene also has, electrochemical window mouth width, electrochemical stability be good, charge transfer
The electrochemical properties such as resistance is small, electro catalytic activity is high and electron transfer rate is fast.Used macrocyclic compound is by 3 to tertiary fourth
Base phenol and 3 half glycoluril units are formed by methylene-bridged, have unique supramolecular chemistry performance.The big cyclisation is closed
The reduced graphene composite material of object functionalization had not only had Supramolecular Recognition performance but also had had excellent electrochemical properties.
Dopamine belongs to Catecholamine matter, is a kind of very important in mammal and mankind's central nervous system
Messenger substance.The study found that human body, which lacks dopamine, will lead to some important diseases, such as alzheimer's disease.It is clinical
On, dopamine is suffered a shock for various types, including toxic shock, cardiogenic shock, hemorrhagic shock, central shock, spy
It is other to renal insufficiency, heart discharge reduce, peripheral vascular resistance is lower and to have supplied the patient of blood volume more intentional
Justice.In recent years, electrochemistry, chemiluminescence, high performance liquid chromatography, surface plasma body resonant vibration, surface-enhanced Raman and glimmering
The method of the detection dopamine such as light method occurs in succession.In the above-mentioned methods, electrochemical process has highly selective, highly sensitive, high
The features such as stability, good biocompatibility, becomes the ideal method of detection dopamine.
Summary of the invention
The object of the present invention is to provide a kind of building of electrochemical sensor and its applications.Sensor of the invention tool
There is easy to operate, response quickly, low in cost, accuracy is high, high sensitivity, the low feature of detection limit, and in stability and again
It makes moderate progress in terms of existing property.
Technical solution of the present invention：A kind of building of electrochemical sensor, is to use molecular formula for C45H60N6O6, structural formula
Reduced graphene composite material is made in macrocyclic compound functionalization reduced graphene as shown in Fig. 1, multiple with reduced graphene
Condensation material modified glassy carbon electrode is to get electrochemical sensor.
The building of electrochemical sensor above-mentioned needs to pre-process before the glass-carbon electrode processing：Method is by glass-carbon electrode
With α-Al2O3Powder polishing grinding dries after being then cleaned by ultrasonic 1-3min with ethyl alcohol and secondary water respectively.
The building of electrochemical sensor above-mentioned, the reduced graphene are prepared via a method which：By graphene oxide
It is placed in secondary water, ultrasound 0.5-1.5h, makes it be dispersed into graphene oxide layer at room temperature；Then ascorbic acid is added,
Stirring at normal temperature 40-60h；Centrifugal filtration discards liquid after stirring, after the secondary water washing of sediment, freeze-drying to get.
The mass ratio of the building of electrochemical sensor above-mentioned, the graphene oxide and ascorbic acid is 1：8-12.
The preparation method of the building of electrochemical sensor above-mentioned, the macrocyclic compound includes the following steps：
1) half glycoluril, p-tert-butylphenol, paraformaldehyde and p-methyl benzenesulfonic acid sulfuric monohydrate is taken to be dissolved in toluene, 60-80
It heating reflux reaction 20-28 hours at DEG C, is cooled to room temperature after the reaction was completed, spins off toluene, obtain A product；
2) A product are dissolved in methylene chloride, are extracted with saturated sodium bicarbonate solution, collected organic phase, use anhydrous magnesium sulfate
It after the dry water removal of powder, filters, discards filter residue, retain filtrate, filtrate is spin-dried for, and obtains B product；
3) by B product silica gel column separating purification, eluant, eluent is ethyl acetate：N-hexane=1:3 solution obtains C product；
4) it takes C product, half glycoluril, paraformaldehyde and p-methyl benzenesulfonic acid sulfuric monohydrate to be dissolved in toluene, adds at 55-65 DEG C
It hot back flow reaction 44-52 hours, is cooled to room temperature after the reaction was completed, spins off toluene, obtain D product；
5) D product are dissolved in the sodium hydroxide solution of 55-65 DEG C of 1mol/L, after being removed by filtration insoluble matter, with hydrochloric acid tune
PH value of solution is saved to neutrality, white precipitate is filtered and is washed with secondary water and methanol to get macrocyclic compound.
The building of electrochemical sensor above-mentioned, in the step 1), in every 100ml toluene containing half glycoluril 0.8-1.2g,
P-tert-butylphenol 2-2.4g, paraformaldehyde 1-1.4g and p-methyl benzenesulfonic acid sulfuric monohydrate 0.4-0.8g；In step 4), often
The 3.0-3.06g of product containing C, one aquation of half glycoluril 0.35-0.39g, paraformaldehyde 0.6-0.7g and p-methyl benzenesulfonic acid in 100ml toluene
Close object 0.4-0.42g.
The method of the building of electrochemical sensor above-mentioned, the macrocyclic compound functionalization reduced graphene is as follows：It will
Reduced graphene is dissolved in dimethyl sulfoxide, at normal temperature ultrasound 0.5-1.5h, and suspension is made, by reduction stone in suspension
Black alkene：Macrocyclic compound=1：3 mass ratio is added macrocyclic compound, to get macrocyclic compound function after stirring at normal temperature 20-28h
The reduced graphene composite material of energyization.
The building of electrochemical sensor above-mentioned, in the reduced graphene composite material, the quality of reduced graphene is dense
Degree is 0.25mg/ml, and the mass concentration of macrocyclic compound is 0.75mg/ml.
The building of electrochemical sensor above-mentioned, it is first that described, which uses reduced graphene composite material modified glassy carbon electrode,
It takes 5 μ L drop coating of reduced graphene composite material on glass-carbon electrode, after solvent volatilization, continues 15 μ L of drop coating, volatilize to solvent
?.
A kind of application of electrochemical sensor above-mentioned in dopamine detection.
Beneficial effects of the present invention
Sensor of the invention and traditional high performance liquid chromatography, surface plasma body resonant vibration, surface-enhanced Raman with
And other detection means such as fluorescence method are compared, which has that easy to operate, response quickly, low in cost, accuracy is high, clever
Sensitivity height and the low advantage of detection limit.Compared with traditional electrochemical sensor, electrochemical sensor of the invention is more stable,
Reproducibility is more preferable.
The electrochemical sensor reported before comparison, such as gold nano grain, graphene and poly- (3,4- second dioxy thiophenes
Pheno) building of poly styrene sulfonate composite material electrochemical sensor, decorative material used in inventive sensor is big ring
The reduced graphene composite material of compound functionalization, composite material preparation of the present invention is easy, easy to operate, low in cost.With
Conduction and amplification by the Supramolecular Recognition performance and reduced graphene of macrocyclic compound to electrochemical signals, the present invention
Sensor application obtains lowest detection and is limited to 6.2 × 10 when the detection of dopamine-4μM, detection limit is reported lower than before
Most of detection for dopamine limit, show its sensitivity with higher.Inventive sensor is further used for detecting
Inotropin, measured rate of recovery range are 99%-113%, this shows that it is applied to actually detected standard with higher
Exactness.It is stored at 4 DEG C after 72h using the electrochemical sensor and is used further to detection dopamine solution, detected unaffected；And it transports
With 10 circle of Cyclic voltammetric method (scanning range is -300-800mV, sweep speed 100mV/s) scanning, final peak current
Only have 5.6% error with initial peak current, shows it with preferable stability.Use the different glass carbon electricity of different five
Pole constructs electrochemical sensor, and the detection error between five sensors is only 4.2%, shows it with preferable reproducibility.
Detailed description of the invention
Attached drawing 1 is the structural schematic diagram of macrocyclic compound of the invention；
Attached drawing 2 is the differential pulse voltammetry figure of electrochemical sensor detection various concentration dopamine of the invention, from figure
It can be seen that the peak position value of dopamine is 0.186V, and peak current increases with the increase of dopamine concentration；
Attached drawing 3 is the differential pulse voltammetry figure according to dopamine, and peak point current is schemed to made by dopamine concentration, by right
The analysis of figure, it is known that concentration and peak point current are presented in the range of dopamine concentration is 0.01-0.1 μM and 5-100 μM
Good linear relationship, equation of linear regression are respectively I (μ A)=+ 0.6869 (r of 96.246C (μM)2=0.9984) and I (μ A)
+ 32.157 (r of=0.3257C (μM)2=0.9626).Inventive sensor is 6.2 × 10 to the minimum detectability of dopamine-4μ
M。
Specific embodiment
Below with reference to embodiment, the present invention is further illustrated, but is not intended as the foundation limited the present invention.
The embodiment of the present invention
Embodiment 1：A kind of building of electrochemical sensor, method are as follows：
One, macrocyclic compound is prepared
1) take half glycoluril 1g, p-tert-butylphenol 2.2g, paraformaldehyde 1.2g and p-methyl benzenesulfonic acid sulfuric monohydrate 0.6g molten
In 100ml toluene, heating reflux reaction 24 hours, are cooled to room temperature after the reaction was completed at 70 DEG C, spin off toluene, obtain A product；
2) A product are dissolved in methylene chloride, are extracted with saturated sodium bicarbonate solution, collected organic phase, use anhydrous magnesium sulfate
It after the dry water removal of powder, filters, discards filter residue, retain filtrate, filtrate is spin-dried for, and obtains B product；
3) by B product silica gel column separating purification, eluant, eluent is ethyl acetate：N-hexane=1:3 solution obtains C product；
4) C product 3.03g, half glycoluril 0.37g, paraformaldehyde 0.65g and p-methyl benzenesulfonic acid sulfuric monohydrate 0.41g is taken to be dissolved in
In 100ml toluene, heating reflux reaction 48 hours, are cooled to room temperature after the reaction was completed at 60 DEG C, spin off toluene, obtain D product；
5) D product are dissolved in the sodium hydroxide solution of 60 DEG C of 1mol/L, after being removed by filtration insoluble matter, are adjusted with hydrochloric acid molten
White precipitate is filtered and is washed with secondary water and methanol to get macrocyclic compound to neutrality by liquid pH.
Two, reduced graphene is prepared
Graphene oxide is placed in secondary water, ultrasound 1h, makes it be dispersed into graphene oxide layer at room temperature；Then
The mass ratio of addition ascorbic acid, graphene oxide and ascorbic acid is 1：10, stirring at normal temperature 50h；It was centrifuged after stirring
It filters and discards liquid, after the secondary water washing of sediment, be freeze-dried to get reduced graphene.
Three, reduced graphene composite material is prepared
Reduced graphene is dissolved in dimethyl sulfoxide, at normal temperature ultrasound 1h, suspension is made, by also in suspension
Former graphene：Macrocyclic compound=1：3 mass ratio is added macrocyclic compound, stirring at normal temperature for 24 hours after to get macrocyclic compound function
The reduced graphene composite material of energyization, in reduced graphene composite material, the mass concentration of reduced graphene is 0.25mg/
Ml, the mass concentration of macrocyclic compound are 0.75mg/ml.
Four, pretreatment of glassy carbon electrode
By glass-carbon electrode α-Al2O3Powder polishing grinding dries in the air after being then cleaned by ultrasonic 2min with ethyl alcohol and secondary water respectively
It is dry.
Five, electrochemical sensor is made
By on the glass-carbon electrode of reduced graphene composite material drop coating after the pre-treatment, after solvent volatilization, continue drop coating 1
It is secondary, it volatilizees to solvent.
Embodiment 2：A kind of building of electrochemical sensor, method are as follows：
One, macrocyclic compound is prepared
1) half glycoluril 0.8g, p-tert-butylphenol 2g, paraformaldehyde 1g and p-methyl benzenesulfonic acid sulfuric monohydrate 0.4g is taken to be dissolved in
In 100ml toluene, heating reflux reaction 20 hours, are cooled to room temperature after the reaction was completed at 60 DEG C, spin off toluene, obtain A product；
2) A product are dissolved in methylene chloride, are extracted with saturated sodium bicarbonate solution, collected organic phase, use anhydrous magnesium sulfate
It after the dry water removal of powder, filters, discards filter residue, retain filtrate, filtrate is spin-dried for, and obtains B product；
3) by B product silica gel column separating purification, eluant, eluent is ethyl acetate：N-hexane=1:3 solution obtains C product；
4) C product 3.0g, half glycoluril 0.35g, paraformaldehyde 0.6g and p-methyl benzenesulfonic acid sulfuric monohydrate 0.4g is taken to be dissolved in
In 100ml toluene, heating reflux reaction 44 hours, are cooled to room temperature after the reaction was completed at 55 DEG C, spin off toluene, obtain D product；
5) D product are dissolved in the sodium hydroxide solution of 55 DEG C of 1mol/L, after being removed by filtration insoluble matter, are adjusted with hydrochloric acid molten
White precipitate is filtered and is washed with secondary water and methanol to get macrocyclic compound to neutrality by liquid pH.
Two, reduced graphene is prepared
Graphene oxide is placed in secondary water, ultrasound 0.5h, makes it be dispersed into graphene oxide layer at room temperature；So
After be added ascorbic acid, the mass ratio of graphene oxide and ascorbic acid is 1：8, stirring at normal temperature 40h；It was centrifuged after stirring
It filters and discards liquid, after the secondary water washing of sediment, be freeze-dried to get reduced graphene.
Three, reduced graphene composite material is prepared
Reduced graphene is dissolved in dimethyl sulfoxide, at normal temperature ultrasound 0.5h, suspension is made, is pressed in suspension
Reduced graphene：Macrocyclic compound=1：Macrocyclic compound is added in 2.5 mass ratio, closes after stirring at normal temperature 20h to get big cyclisation
The reduced graphene composite material of object functionalization, in reduced graphene composite material, the mass concentration of reduced graphene is
0.25mg/ml, the mass concentration of macrocyclic compound are 0.75mg/ml.
Four, pretreatment of glassy carbon electrode
By glass-carbon electrode α-Al2O3Powder polishing grinding dries in the air after being then cleaned by ultrasonic 1min with ethyl alcohol and secondary water respectively
It is dry.
Five, electrochemical sensor is made
By on the glass-carbon electrode of reduced graphene composite material drop coating after the pre-treatment, after solvent volatilization, continue drop coating 1
It is secondary, it volatilizees to solvent.
Embodiment 3：A kind of building of electrochemical sensor, method are as follows：
One, macrocyclic compound is prepared
1) half glycoluril 1.2g, p-tert-butylphenol 2.4g, paraformaldehyde 1.4g and p-methyl benzenesulfonic acid sulfuric monohydrate 0.8g are taken
It is dissolved in 100ml toluene, heating reflux reaction 28 hours, are cooled to room temperature after the reaction was completed at 80 DEG C, spin off toluene, obtain A
Product；
2) A product are dissolved in methylene chloride, are extracted with saturated sodium bicarbonate solution, collected organic phase, use anhydrous magnesium sulfate
It after the dry water removal of powder, filters, discards filter residue, retain filtrate, filtrate is spin-dried for, and obtains B product；
3) by B product silica gel column separating purification, eluant, eluent is ethyl acetate：N-hexane=1:3 solution obtains C product；
4) C product 3.06g, half glycoluril 0.39g, paraformaldehyde 0.7g and p-methyl benzenesulfonic acid sulfuric monohydrate 0.42g is taken to be dissolved in
In 100ml toluene, heating reflux reaction 52 hours, are cooled to room temperature after the reaction was completed at 65 DEG C, spin off toluene, obtain D product；
5) D product are dissolved in the sodium hydroxide solution of 65 DEG C of 1mol/L, after being removed by filtration insoluble matter, are adjusted with hydrochloric acid molten
White precipitate is filtered and is washed with secondary water and methanol to get macrocyclic compound to neutrality by liquid pH.
Two, reduced graphene is prepared
Graphene oxide is placed in secondary water, ultrasound 1.5h, makes it be dispersed into graphene oxide layer at room temperature；So
After be added ascorbic acid, the mass ratio of graphene oxide and ascorbic acid is 1：12, stirring at normal temperature 60h；It is centrifuged after stirring
It filters and discards liquid, after the secondary water washing of sediment, be freeze-dried to get reduced graphene.
Three, reduced graphene composite material is prepared
Reduced graphene is dissolved in dimethyl sulfoxide, at normal temperature ultrasound 1.5h, suspension is made, is pressed in suspension
Reduced graphene：Macrocyclic compound=1：Macrocyclic compound is added in 3.5 mass ratio, closes after stirring at normal temperature 28h to get big cyclisation
The reduced graphene composite material of object functionalization, in reduced graphene composite material, the mass concentration of reduced graphene is
0.25mg/ml, the mass concentration of macrocyclic compound are 0.75mg/ml.
Four, pretreatment of glassy carbon electrode
By glass-carbon electrode α-Al2O3Powder polishing grinding dries in the air after being then cleaned by ultrasonic 3min with ethyl alcohol and secondary water respectively
It is dry.
Five, electrochemical sensor is made
By on the glass-carbon electrode of reduced graphene composite material drop coating after the pre-treatment, after solvent volatilization, continue drop coating 1
It is secondary, it volatilizees to solvent.
Embodiment 4：The application of above-mentioned electrochemical sensor, is the application in dopamine detection, and detection method is as follows：
(1) dopamine solution is prepared：Methanol prepares various concentration dopamine solution as solvent, and concentration is 1.0 × 10- 8mol/L-1.0×10-4mol/L；
(2) constructed electrochemical sensor is put into the dopamine solution of each concentration after room temperature incubation 40min, is proposed
It dries, obtains electrode to be measured；
(3) using electrode to be measured as working electrode, Ag/AgCl electrode is saturated as reference electrode, platinum electrode is as auxiliary
Electrode builds three-electrode system, is measured using electrochemical workstation.Wherein, used electrochemical test method is poor
Divide pulse voltammetry, parameter is：Potential range is 0-400mV, pulse width 50ms, pulse period 200ms, pulse amplitude
For 50mV.
Claims (10)
1. a kind of building of electrochemical sensor, it is characterised in that：It is to use molecular formula for C45H60N6O6, structural formula is：
Macrocyclic compound functionalization reduced graphene, be made reduced graphene composite material, with reduced graphene composite material
Modified glassy carbon electrode is to get electrochemical sensor.
2. the building of electrochemical sensor according to claim 1, it is characterised in that：It is needed before the glass-carbon electrode processing pre-
Processing：Method is by glass-carbon electrode α-Al2O3Then powder polishing grinding is cleaned by ultrasonic 1-3min with ethyl alcohol and secondary water respectively
After dry.
3. the building of electrochemical sensor according to claim 1, it is characterised in that：The reduced graphene passes through as follows
Method preparation：Graphene oxide is placed in secondary water, ultrasound 0.5-1.5h, makes it be dispersed into graphene oxide sheet at room temperature
Layer；Then ascorbic acid, stirring at normal temperature 40-60h is added；Centrifugal filtration discards liquid, sediment secondary water after stirring
After washing, freeze-drying to get.
4. the building of electrochemical sensor according to claim 3, it is characterised in that：The graphene oxide and Vitamin C
The mass ratio of acid is 1：8-12.
5. the building of electrochemical sensor according to claim 1, which is characterized in that the preparation side of the macrocyclic compound
Method includes the following steps：
1) half glycoluril, p-tert-butylphenol, paraformaldehyde and p-methyl benzenesulfonic acid sulfuric monohydrate is taken to be dissolved in toluene, at 60-80 DEG C
It heating reflux reaction 20-28 hours, is cooled to room temperature after the reaction was completed, spins off toluene, obtain A product；
2) A product are dissolved in methylene chloride, are extracted with saturated sodium bicarbonate solution, organic phase is collected, with anhydrous slufuric acid magnesium dust
It after dry water removal, filters, discards filter residue, retain filtrate, filtrate is spin-dried for, and obtains B product；
3) by B product silica gel column separating purification, eluant, eluent is ethyl acetate：N-hexane=1:3 solution obtains C product；
4) it takes C product, half glycoluril, paraformaldehyde and p-methyl benzenesulfonic acid sulfuric monohydrate to be dissolved in toluene, is heated back at 55-65 DEG C
Stream reaction 44-52 hours, is cooled to room temperature after the reaction was completed, spins off toluene, obtain D product；
5) D product are dissolved in the sodium hydroxide solution of 55-65 DEG C of 1mol/L, after being removed by filtration insoluble matter, are adjusted with hydrochloric acid molten
White precipitate is filtered and is washed with secondary water and methanol to get macrocyclic compound to neutrality by liquid pH.
6. the building of electrochemical sensor according to claim 5, it is characterised in that：In the step 1), every 100ml first
Contain half glycoluril 0.8-1.2g, p-tert-butylphenol 2-2.4g, paraformaldehyde 1-1.4g and p-methyl benzenesulfonic acid sulfuric monohydrate in benzene
0.4-0.8g；In step 4), the 3.0-3.06g of product containing C, half glycoluril 0.35-0.39g, paraformaldehyde 0.6- in every 100ml toluene
0.7g and p-methyl benzenesulfonic acid sulfuric monohydrate 0.4-0.42g.
7. the building of electrochemical sensor according to claim 1, it is characterised in that：The macrocyclic compound functionalization is also
The method of former graphene is as follows：Reduced graphene is dissolved in dimethyl sulfoxide, ultrasound 0.5-1.5h, is made suspended at normal temperature
Liquid presses reduced graphene in suspension：Macrocyclic compound=1：Macrocyclic compound, stirring at normal temperature 20- is added in 3 mass ratio
To get the reduced graphene composite material of macrocyclic compound functionalization after 28h.
8. the building of electrochemical sensor according to claim 7, it is characterised in that：The reduced graphene composite material
In, the mass concentration of reduced graphene is 0.25mg/ml, and the mass concentration of macrocyclic compound is 0.75mg/ml.
9. the building of electrochemical sensor according to claim 1, it is characterised in that：Described is compound with reduced graphene
Material modified glassy carbon electrode is first to take 5 μ L drop coating of reduced graphene composite material on glass-carbon electrode, after solvent volatilization, is continued
15 μ L of drop coating volatilize to solvent.
10. a kind of application of -9 described in any item electrochemical sensors according to claim 1 in dopamine detection.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110346434A (en) * | 2019-05-30 | 2019-10-18 | 贵州大学 | A kind of reduced graphene composite material and its electrochemistry chiral sensor of building |
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| CN107741445A (en) * | 2017-08-30 | 2018-02-27 | 河海大学 | A kind of screen printing electrode and its preparation method and application |
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2018
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|---|---|---|---|---|
| US8962605B2 (en) * | 2009-08-12 | 2015-02-24 | Technion Research & Development Foundation Limited | Polycyclic compounds, termed calixurenes, and uses thereof |
| CN107741445A (en) * | 2017-08-30 | 2018-02-27 | 河海大学 | A kind of screen printing electrode and its preparation method and application |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110346434A (en) * | 2019-05-30 | 2019-10-18 | 贵州大学 | A kind of reduced graphene composite material and its electrochemistry chiral sensor of building |
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Application publication date: 20181116 |