CN108827958A - For quickly detecting the kit and its detection method of magnesium chloride in edible mushroom - Google Patents
For quickly detecting the kit and its detection method of magnesium chloride in edible mushroom Download PDFInfo
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- CN108827958A CN108827958A CN201810973907.4A CN201810973907A CN108827958A CN 108827958 A CN108827958 A CN 108827958A CN 201810973907 A CN201810973907 A CN 201810973907A CN 108827958 A CN108827958 A CN 108827958A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/82—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a precipitate or turbidity
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N2021/775—Indicator and selective membrane
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Abstract
The invention discloses a kind of for quickly detecting the kit and its detection method of edible mushroom in agaric, and this method includes：Edible mushroom is shredded and is put into specimen cup, distilled water immersion is added, for several times, standing takes supernatant as prepare liquid for concussion；The first step takes prepare liquid into No. 1 centrifuge tube, and the sodium hydroxide solution containing azoviolet is added, and shakes up observation color change, and blue is presented or has blue precipitate generation, illustrates to contain magnesium ion in prepare liquid, and into second step, otherwise experiment terminates；Second step is added potassium chromate solution and silver nitrate solution mixes, generate reddish-brown precipitation in No. 2 centrifuge tubes, prepare liquid is added, shakes up standing, reddish-brown precipitation does not disappear, chloride ion is not contained in prepare liquid, reddish-brown precipitation disappears, then contains chloride ion in prepare liquid.The purpose of the present invention is to provide a kind of kits and its detection method for magnesium chloride in quickly detection edible mushroom that can be quickly detected and to the magnesium chloride added in existing edible mushroom.
Description
Technical field
The present invention relates to field of detection of food safety more particularly to a kind of for quickly detecting the reagent of edible mushroom in agaric
Box and its detection method.
Background technique
Magnesium sulfate and magnesium chloride are all to belong to inorganic chemical product, and magnesium sulfate is used to deploy in medicine protection ointment, rushes down
Agent, analgestic, antidote are also used in process hides, production fertilizer, Industrial Waste Water Treatments etc..Magnesium chloride is generally used for system gold
Belong to magnesium, disinfectant, extinguishing chemical, chilled brine, ceramics, and is used for fabric and papermaking etc..One in magnesium originally human body
Kind nutrient, but it cannot function as food additives use；If intake is excessive, the corrosion of mucous membrane can be caused, nausea is caused to be vomitted
The symptoms such as Tuhe diarrhea, or even aggravate the damage of kidney and liver.
Inspection has many kits for detecting magnesium sulfate in the market fastly at present, and principle is usually in difference Qualitive test agaric
Whether contain magnesium ion and sulfate ion, thus judges whether mix magnesium sulfate in agaric.
Existing edible mushroom magnesium sulfate kit, is divided into two classes, and the first kind is most commonly seen, and principle is difference test sample
In whether contain magnesium ion and sulfate ion, two results can determine whether to contain magnesium sulfate in sample when being the positive, wherein one
It when being positive, can determine whether that magnesium sulfate may be contained in sample, two when being feminine gender, can determine whether in sample without containing sulfuric acid
Magnesium；Second class, more rare, principle is whether to contain magnesium ion or sulfate ion in test sample, thus judgement sample
In whether contain magnesium sulfate.
The above two classes product is sensitive there are many ways to use due to it when detecting magnesium ion and sulfate ion
Degree, specificity, product form mode are different；It is all whether to add sulfuric acid according in edible mushroom but regardless of method
Magnesium and design, can not judge whether contain magnesium chloride in edible mushroom.And there is no magnesium chlorides in special detection edible mushroom at present
Fast inspection product.
Summary of the invention
The purpose of the present invention is to provide a kind of for quickly detecting the kit of magnesium chloride and its detection side in edible mushroom
Method can quickly detect the magnesium chloride added in existing edible mushroom to come, compensate in the prior art without special detection
The deficiency of the fast inspection product of magnesium chloride in edible mushroom.
The technical solution adopted by the present invention is as follows：It is a kind of for quickly detecting the kit of magnesium chloride in edible mushroom, the examination
Agent box includes liquid A bottles of detection, liquid B bottles of detection, liquid C bottles of detection, two centrifuge tubes and specimen cup, interior installed reagents in liquid A bottles of detection
For 0.01 ~ 0.15g/L azoviolet, solvent is 0.5 ~ 5mol/L sodium hydroxide；Installed reagents are 1 ~ 25g/L chromic acid in liquid B bottles of detection
Potassium, solvent are distilled water；Installed reagents are 2 ~ 50g/L silver nitrate in liquid C bottles of detection, and solvent is distilled water；
Before detection, edible mushroom is shredded and is put into specimen cup, distilled water is added, impregnate 5 ~ 10min, during which concussion for several times, is stood
After take supernatant as prepare liquid；Detection is carried out in two steps, the first step, takes prepare liquid into No. 1 centrifuge tube, and detection liquid A is added,
Color change is observed after shaking up, blue is presented or has blue precipitate generation, illustrates to contain magnesium ion in prepare liquid, into second step
Experiment, if liquid is not blue also generated without blue precipitate if test and terminate；Detection is added in No. 2 centrifuge tubes in second step
Liquid B and detection liquid C, mixes, generates reddish-brown precipitation, prepare liquid is added, stands after shaking up, reddish-brown precipitation does not disappear, to be measured
Chloride ion is not contained in liquid, reddish-brown precipitation disappears, then contains chloride ion in prepare liquid.
It is a kind of for quickly detecting the detection method of magnesium chloride in edible mushroom, the detection method used detection liquid A, detection
Liquid B and detection liquid C, wherein detection liquid A is 0.01 ~ 0.15g/L azoviolet, solvent is 0.5 ~ 5mol/L sodium hydroxide, detects liquid B
For 1 ~ 25g/L potassium chromate, solvent is distilled water, and detection liquid C is 2 ~ 50/L silver nitrate, and solvent is distilled water；The detection method
Steps are as follows：
Step 01：Edible mushroom to be detected is shredded, is put into specimen cup, distilled water is added, impregnates 5 ~ 10min, is during which shaken
For several times, take supernatant as prepare liquid after standing；
Step 02：It takes prepare liquid into No. 1 centrifuge tube, detection liquid A is added, shakes up observation color change；If liquid becomes blue
Or have blue precipitate generation, then illustrate to contain magnesium ion in prepare liquid, continues experiment and enter step 03；If the constant indigo plant of liquid
Color is also generated without blue precipitate, illustrates not containing magnesium ion in the prepare liquid, magnesium chloride, experiment are not contained in the edible mushroom
Terminate；
Step 03：In No. 2 centrifuge tubes, be added detection liquid B and detection liquid C, reddish-brown precipitation is generated after shaking up, be then added to
Liquid is surveyed, and shakes up standing, if reddish-brown precipitation disappears, reaction solution turns yellow, then illustrates to contain chloride ion in prepare liquid, in turn
It can be concluded that containing magnesium chloride in the edible mushroom sample；Conversely, illustrating in the edible mushroom sample if reddish-brown precipitation does not disappear
Without containing magnesium chloride.
0.1 ~ 1ml of prepare liquid is added in step 02, it is 1 ~ 4 drop that detection liquid A, which is added,.
The molal quantity for the potassium chromate being added in step 03 is 0.5 times of silver nitrate molal quantity, and 0.1 ~ 1ml is added in prepare liquid.
The good effect that technical solution of the present invention generates is as follows：Kit and detection method of the invention, passes through two steps
Experiment detects whether can judge to eat if the positive is presented in the result of two steps containing magnesium ion and chloride ion in edible mushroom
With having used magnesium chloride in bacterium；The experiment of two steps is obtained a result by observing color change, intuitive quick and easy to use；This
Invention solves the disadvantage that in the prior art the magnesium chloride testing product of not complete set.
Specific embodiment
The technical solution of kit and detection method of the invention is further elaborated combined with specific embodiments below
Explanation.
For quickly detecting the kit and detection method of magnesium chloride in edible mushroom, the edible mushroom in the present invention be agaric,
The fresh sample of the edible mushrooms such as tremella, mushroom, dictyophora phalloidea, agrocybe and dry sample.The kit includes liquid A bottles of detection, liquid B bottles of detection, detection
The centrifuge tube of C bottles, two 1.5mL of liquid and the specimen cup of a 40mL；
Liquid A bottles of detection：Reagent bottle is 12mL black drop bottle, and interior installed reagents are 0.13g/L azoviolet, and solvent is 2.5mol/L hydrogen-oxygen
Change sodium；
Liquid B bottles of detection：Reagent bottle is 12mL black drop bottle, and interior installed reagents are 4.855g/L potassium chromate, and solvent is distilled water；
Liquid C bottles of detection：Reagent bottle is 12mL black drop bottle, and interior installed reagents are 8.5g/L silver nitrate, and solvent is distilled water.
Detection method for quickly detecting magnesium chloride in edible mushroom is as follows：
Step 01：First carries out sample treatment before detection, chooses dried fungus as edible mushroom sample, agaric is shredded, weigh 1g in
In specimen cup, 20mL distilled water is added, impregnates 5 ~ 10min, during which oscillation for several times, takes supernatant as prepare liquid after standing；
Step 02：It takes in prepare liquid 0.5mL to No. 1 centrifuge tube, 2 drops is added（1 drop is equivalent to 50 μ L in the present invention）Liquid A is detected,
Color change is observed after shaking up, if being displayed in blue or having blue precipitate generation, illustrates to contain magnesium ion in the prepare liquid, is continued
Next step experimental procedure 03；If liquid is not blue without blue precipitate yet, liquid is displayed in red or purple or reddish violet, should be to
It surveys and does not contain magnesium ion in liquid, therefore illustrate that the agaric does not add magnesium chloride, experiment terminates；
Magnesium ion is reacted with sodium hydroxide generates magnesium hydroxide, is white precipitate, but when magnesium ion content is lower, naked eyes may
It can not directly observe and carry out white precipitate, azoviolet is added（It is azoviolet I used in the present embodiment）, azoviolet absorption hydrogen-oxygen
Changing magnesium can produce blue precipitate, and when magnesium hydroxide is less, solution is displayed in blue, when magnesium hydroxide content is high, i.e., considerable
Blue precipitate is observed, such detection method is intuitive and accurate；As not having magnesium ion in solution, then without above-mentioned reaction, solution is in
Existing color is still the reddish violet of azoviolet I.
Step 03：In No. 2 centrifuge tubes, 2 drop detection liquid B are added, 2 drops detect liquid C, mix, and generate reddish-brown precipitation；So
The prepare liquid 0.5mL in step 01 is added afterwards, stands 2 minutes after shaking up, if reddish-brown precipitation does not disappear, illustrates in prepare liquid
Without containing chloride ion, then illustrate in the sample agaric without containing magnesium chloride；If reddish-brown precipitation disappears, yellow is presented in liquid, then
Illustrate to contain chloride ion in prepare liquid, the positive is presented therefore, it is determined that containing magnesium chloride in sample agaric in the detection of two steps.
The nitric acid silver reaction detected in potassium chromate and detection the liquid C in liquid B generates siliver chromate russet precipitating, then plus
Enter prepare liquid, if siliver chromate russet precipitating disappears, illustrates to contain chloride ion in prepare liquid, silver nitrate and chromic acid nak response
Brick-red siliver chromate precipitating is generated, siliver chromate precipitating encounters the chromate that chloride ion generates silver chlorate white precipitate and yellow
（Because of Ag2CrO4Solubility ratio AgCl it is small）.Reaction equation is as follows：2AgNO3+K2CrO4=Ag2CrO4↓（Reddish-brown precipitation）+
2KNO3；Ag2CrO4+2Cl--=2AgCl↓（White precipitate）+CrO4 2-（Yellow）.
The amount for being arranged and being added when specifically used that liquid concentration is detected in the present embodiment is a kind of preferable embodiment, but
It is all based on the technical solutions of the present invention, varied concentration setting and usage amount falls in the implementation in concentration range of the present invention
Mode is within the scope of the invention.
Claims (4)
1. a kind of for quickly detecting the kit of magnesium chloride in edible mushroom, it is characterised in that：The kit includes detection liquid A
Bottle, liquid B bottles of detection, liquid C bottles of detection, two centrifuge tubes and specimen cup, detecting interior installed reagents in liquid A bottles is 0.01 ~ 0.15g/L magnesium
Reagent, solvent are 0.5 ~ 5mol/L sodium hydroxide；Installed reagents are 1 ~ 25g/L potassium chromate in liquid B bottles of detection, and solvent is distilled water；
Installed reagents are 2 ~ 50g/L silver nitrate in liquid C bottles of detection, and solvent is distilled water；
Before detection, edible mushroom is shredded and is put into specimen cup, distilled water is added, impregnate 5 ~ 10min, during which concussion for several times, is stood
After take supernatant as prepare liquid；Detection is carried out in two steps, the first step, takes prepare liquid into No. 1 centrifuge tube, and detection liquid A is added,
Color change is observed after shaking up, blue is presented or has blue precipitate generation, illustrates to contain magnesium ion in prepare liquid, into second step
Experiment, if liquid is not blue also generated without blue precipitate if test and terminate；Detection is added in No. 2 centrifuge tubes in second step
Liquid B and detection liquid C, mixes, generates reddish-brown precipitation, prepare liquid is added, stands after shaking up, reddish-brown precipitation does not disappear, to be measured
Chloride ion is not contained in liquid, reddish-brown precipitation disappears, then contains chloride ion in prepare liquid.
2. a kind of for quickly detecting the detection method of magnesium chloride in edible mushroom, it is characterised in that：The detection method has used inspection
Liquid A, detection liquid B and detection liquid C are surveyed, wherein detection liquid A is 0.01 ~ 0.15g/L azoviolet, solvent is 0.5 ~ 5mol/L hydroxide
Sodium, detection liquid B are 1 ~ 25g/L potassium chromate, and solvent is distilled water, and detection liquid C is 2 ~ 50/L silver nitrate, and solvent is distilled water；It should
The step of detection method, is as follows：
Step 01：Edible mushroom to be detected is shredded, is put into specimen cup, distilled water is added, impregnates 5 ~ 10min, is during which shaken
For several times, take supernatant as prepare liquid after standing；
Step 02：It takes prepare liquid into No. 1 centrifuge tube, detection liquid A is added, shakes up observation color change；If liquid becomes blue
Or have blue precipitate generation, then illustrate to contain magnesium ion in prepare liquid, continues experiment and enter step 03；If the constant indigo plant of liquid
Color is also generated without blue precipitate, illustrates not containing magnesium ion in the prepare liquid, magnesium chloride, experiment are not contained in the edible mushroom
Terminate；
Step 03：In No. 2 centrifuge tubes, be added detection liquid B and detection liquid C, reddish-brown precipitation is generated after shaking up, be then added to
Liquid is surveyed, and shakes up standing, if reddish-brown precipitation disappears, reaction solution turns yellow, then illustrates to contain chloride ion in prepare liquid, in turn
It can be concluded that containing magnesium chloride in the edible mushroom sample；Conversely, illustrating in the edible mushroom sample if reddish-brown precipitation does not disappear
Without containing magnesium chloride.
3. according to claim 2 for quickly detecting the detection method of magnesium chloride in edible mushroom, it is characterised in that：Step
0.1 ~ 1ml of prepare liquid is added in 02, it is 1 ~ 4 drop that detection liquid A, which is added,.
4. according to claim 2 for quickly detecting the detection method of magnesium chloride in edible mushroom, it is characterised in that：Step
The molal quantity for the potassium chromate being added in 03 is 0.5 times of silver nitrate molal quantity, and 0.1 ~ 1ml is added in prepare liquid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810973907.4A CN108827958A (en) | 2018-08-24 | 2018-08-24 | For quickly detecting the kit and its detection method of magnesium chloride in edible mushroom |
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| CN201810973907.4A CN108827958A (en) | 2018-08-24 | 2018-08-24 | For quickly detecting the kit and its detection method of magnesium chloride in edible mushroom |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112986230A (en) * | 2021-03-02 | 2021-06-18 | 郑州欧柯奇仪器制造有限公司 | Kit and method for rapidly detecting magnesium sulfate in agaric |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105092572A (en) * | 2015-08-27 | 2015-11-25 | 上海市农业科学院 | Method for detecting magnesium chloride in edible mushrooms |
-
2018
- 2018-08-24 CN CN201810973907.4A patent/CN108827958A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105092572A (en) * | 2015-08-27 | 2015-11-25 | 上海市农业科学院 | Method for detecting magnesium chloride in edible mushrooms |
Non-Patent Citations (3)
| Title |
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| 徐风广 等: "《无机非金属材料化验与检测实训》", 31 July 2018, 中国建材工业出版社 * |
| 邱星群: ""增肥"黑木耳中硫酸镁的检测", 《科技信息》 * |
| 陆锦昌 等: "《丝绸染整手册 第二版》", 31 October 1982, 中国纺织出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112986230A (en) * | 2021-03-02 | 2021-06-18 | 郑州欧柯奇仪器制造有限公司 | Kit and method for rapidly detecting magnesium sulfate in agaric |
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