CN108823269A - The preparation method of fish collagen oligopeptide - Google Patents
The preparation method of fish collagen oligopeptide Download PDFInfo
- Publication number
- CN108823269A CN108823269A CN201810473935.XA CN201810473935A CN108823269A CN 108823269 A CN108823269 A CN 108823269A CN 201810473935 A CN201810473935 A CN 201810473935A CN 108823269 A CN108823269 A CN 108823269A
- Authority
- CN
- China
- Prior art keywords
- fish
- skin
- added
- deodorant
- collagen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]
Abstract
The invention discloses the preparation methods of fish collagen oligopeptide, specially：Tissue mashing after fish-skin is shredded is added in oxygen sodium hydroxide solution, impregnates and constantly stirs, is then washed to neutrality, adds aqueous isopropanol immersion, filters after cleaning to neutrality, sufficiently drain, is homogenized, spare；Active dry yeast and arabinose will be added in homogenate, then deodorant is added beta-cyclodextrin progress super-pressure defishying and obtains the homogenate of deodorant fish-skin；The pH of deodorant homogenate is adjusted, compound protease, sodium stearate and tetrabutylammonium bromide is added, ultrasonic wave digests, and nanofiltration membrane nanofiltration is used in enzymolysis process, and nanofiltration liquid is spray-dried to get fish collagen oligopeptide.It has the beneficial effect that：Present invention preparation side is low, easy to operate with investment, collagen degradation effect is good, the oligomeric peptide yield of collagen is high, the higher advantage of sensory-acceptance, is easy to industrial-scale production, has good application value and market potential.
Description
Technical field
The present invention relates to bioactive ingredients technical fields, the in particular to preparation method of fish collagen oligopeptide.
Background technique
Collagen（Collagen）It is extracellular matrix（ECM）A kind of structural proteins, be made of containing one or several α chain
Triple-helix structure region, also known as collagen domain.Collagen is a kind of important structural proteins in animal tissue, by animal at fibre
The macromolecular fibre type protein of cell synthesis, is widely present in animal tendon, ligament, cartilage, skin and other connective tissues
In, play the important physiological functions such as support, nutrition, immune, protection, information transmitting.The structure of all kinds of collagens has significant general character,
Its primary structure shows as glycyl-prolyl-Y, glycyl-prolyl-hydroxyprolyl-, glycyl-X-Y（Wherein X, Y are represented
Any other amino acid residue in addition to prolyl and glycyl）Tripeptides repetitive sequence.Collagen contains in other protein
Rare hydroxyproline and hydroxylysine also has more proline and lysine, lacks tryptophan, ArAA and half Guang
Histidine content is less.Secondary structure shows as special triple helix structure, be by α-peptide chain of three left hand helixs with it is parallel,
Dextrorotatory form is wound cord-like right-handed superhelix structure.Tertiary structure discloses collagen peptide chain in addition to secondary key effect, molecule
It is interior with intermolecular there is also three kinds of aldol condensations, aldehyde alcohol histidine and aldimine condensation cross-linked forms.Quaternary structure shows as virgin rubber
Original molecule arranges bunchy according to certain regular parallel, and head and the tail stagger arrangement a quarter overlaps to form stable collagen fibre with covalent bond
Dimension, is further gathered into bigger fibre bundle.
The thermal stability of fish collagen is lower and presents fingerling specificity, i.e., warm water fish collagen is compared with cold water fish collagen
Thermal stability is high, this is related with its living environment, and also difference is related due to fingerling, tissue site are different with hydroxyproline content.
Compared with terrestrial animal, fish glue original acid composition and content have the one side of general character, and there are significant particularity, methionine contains
Amount more than terrestrial animal and proline, hydroxyproline content are relatively fewer.And the content of hydroxyproline, proline is lower, glue
The temperature that original structure is destroyed is lower, this is because hydroxyproline is in connection peptide chain and stablizes collagen helix configuration aspects
Important function.In recent years, all kinds of collagens are in beauty and health care, medical and health, field of food, organization material engineering and biomaterial
Equal middle and high ends field shows good application development prospect.Collagen peptide as a kind of biologically active peptide, be between protein and
Molecularly Imprinted Polymer between amino acid.Usually collagen oligopeptide will be known as by the collagen peptide of 2~10 Amino acid profiles
（Collagenous Oligopeptide）, molecular weight is 1000Da or less.By the referred to as glue of 10~100 Amino acid profiles
Former polypeptide.Modern research suggests that for polypeptide is compared with protein, it is easier to digest and assimilate, energy can be provided, without egg to body rapidly
The features such as white matter denaturation, hypoallergenic, good water solubility, and small molecule oligopeptide may not need digestion, directly be inhaled by body
It receives and utilizes, there is multi-biological active function.In recent years, all kinds of correlative studys of domestic and international collagen peptide develop rapidly.
Summary of the invention
The purpose of the present invention is to provide a kind of extraction rate is fast, fish collagen oligopeptide yield and activity is high, sense organ receives
It spends, the preparation method of the fish collagen oligopeptide of good quality.
The present invention in view of the above technology in the problem of mentioning, the technical solution taken is：
The preparation method of fish collagen oligopeptide, including pretreatment, deodorant, enzymatic hydrolysis, the specific steps are that：
Pretreatment：Tissue mashing after fish-skin is shredded is 1 by solid-liquid ratio:It is 0.08-0.13M that concentration, which is added, in fish-skin by 4-6g/mL
Oxygen sodium hydroxide solution in, impregnate and constantly stir, rinsed with circulating water to neutrality after 1.5-2.5h, then by solid-liquid ratio be 1:
The aqueous isopropanol that concentration is 2-5% is added in 1.5-2.5g/mL, is cleaned repeatedly with distilled water after impregnating 10-15h to neutral rear yarn
Cloth filtering, sufficiently drains, then is by weight 1:Deionized water is added into the fish-skin drained by 5-7, homogenate, spare, fish glue from skin
Former polypeptide before preparation, because wherein contain many foreign proteins and fat, so first to carry out pre-treatment remove these foreign proteins,
Fat etc. can reduce influence of the substance of this kind to fishskin collagen polypeptide；
Deodorant：The Arab of the active dry yeast and fish-skin weight 0.1-0.3% of fish-skin weight 0.8-1.3% will be added in homogenate
Then the beta-cyclodextrin of fish-skin weight 5-8% is added in sugar, the deodorant 20-30min at 38-43 DEG C, be 60-70 DEG C, surpass in temperature
Super-pressure defishying 10-20min dredges under conditions of high pressure is 300-400MPa to get the homogenate of deodorant fish-skin, active dry yeast
The structure of pine has suction-operated, while active dry yeast enzyme containing there are many to stench substance, first uses active dry yeast in the step
It converts stench substance to without substance of smelling as of rotten fish, then the macromolecular substances such as substance such as aldehyde, ketone of smelling as of rotten fish recycle β-by cell accumulation
The embedding effect of cyclodextrine removes remaining yeast fermented flavour and fermentation acid and remaining fishy smell, reaches more preferably except raw meat is imitated
Fruit improves the sensory-acceptance of target product；
Enzymatic hydrolysis：The pH for adjusting deodorant homogenate is 7.5-8.5, is that compound protease is added in 4.5-5.5% by enzyme concentration, adds ten
Eight sour sodium and tetrabutylammonium bromide, ultrasonic wave digests 5- under conditions of temperature is 30-40 DEG C, ultrasonic power is 200-300W
8h, nanofiltration membrane nanofiltration is used in enzymolysis process, and nanofiltration liquid is spray-dried to get fish collagen oligopeptide.Different protease act on egg
Site on white matter peptide chain is different, causes composition difference of the Degree of Enzymatic Hydrolysis with polypeptide, present invention ultrasonic wave assisted recombination egg
White enzyme is digested, and Isin glue collagen can be made sufficiently to digest, and improves the content of fish collagen oligopeptide in enzymolysis liquid, while the step
Suddenly has many advantages, such as mild enzymatic hydrolysis condition, reaction process easy to control, at low cost, in enzymolysis process, have no toxic side effect generation.
Preferably, the additive amount of sodium stearate is the 2-2.6% of compound protease weight, tetrabutyl bromine in enzymolysis step
The additive amount for changing ammonium is the 0.3-0.5% of compound protease weight.The special presence of sodium stearate and tetrabutylammonium bromide can not only
The hydrophobic region of Isin glue collagen is enough acted on, dispersibility and stability of the Isin glue collagen in the homogenate of collagen meat is improved, mentions
The touch opportunity of high Isin glue collagen and protease, and then enzymatic hydrolysis rate and collagen peptide yield are improved, and sugared egg can be cut
White glycosidic bond, makes glycogen degradation, so that protease quickly identifies that the albumen for the glycogen that is removed is digested, and is finally mentioned
The yield of high collagen peptide.
Preferably, compound protein is clostridiopetidase A and neutral proteinase, weight ratio 1 in enzymolysis step:4-5.The step
Suddenly it is digested using clostridiopetidase A and neutral proteinase, clostridiopetidase A can exclusively be beaten under the premise of not destroying other protein
The distinctive superhelix of collagen is opened, and neutral proteinase can carry out digestion from different amino acid sites, to generate
A large amount of fish collagen oligopeptide, while leading to reinfocing effect due to synergistic function between enzyme, the fish glue accordingly generated
Collagen oligopeptide segment function can further enhance, collagen oligopeptide in the final enzymolysis efficiency for improving fish glue albumen, enzymolysis liquid
Content and activity.
Preferably, the time interval of nanofiltration is 25-35min in enzymolysis step, nanofiltration retaining molecular weight is 1kDa, is received
The filter time is 10-20min.Small molecule product is removed with nanofiltration during the reaction, can reduce the dense of product in enzymatic hydrolysis system
Degree, so that enzyme digestion reaction rate can continue to keep, greatly shortens enzymolysis time.
Preferably, containing the D-arabinose of 4.3-4.6% in deodorant step in arabinose.The presence of arabinose
Energy consumption can be provided for active dry yeast, the metabolism amount of alcohol dehydrogenase and aldehyde dehydrogenase is improved, by fishy smell substance E-2- decenal
It is converted to E-2- decenol and E-2- decylenic acid, improves the quality of deodorization effect and target product, while being avoided that the dry ferment of activity
Mother consumption clam albumen, improve the yield of target product, at the same in arabinose D-arabinose special presence so that different
The collagen of structure is very sensitive to pressure, so that the construction of collagen molecules is changed, is denaturalized, so that internal
Restriction enzyme site exposure, be easy to by protease digestion, while fish growth environment keeps itself contained micro organism quantity big, collagen egg
In white enzymolysis process mild condition be conducive to microbial growth, by product quality and yield have an adverse effect, and
D-arabinose cooperation super-pressure effect can change the Premeabilisation of cells pressure of microorganism, kills this quasi-microorganism, it is low to improve collagen
The yield and quality of poly- peptide.
Compared with the prior art, the advantages of the present invention are as follows：1）Present invention preparation side, which has, puts into low, easy to operate, glue
Former protein degradation effect is good, the oligomeric peptide yield of collagen is high, the higher advantage of sensory-acceptance, is easy to industrial-scale production, has
There are good application value and market potential；2）The extracting method deodorization effect is excellent, and the sense organ for improving collagen oligopeptide receives
Degree can make the restriction enzyme site exposure inside collagen during deodorant, be easy to that the thin of microorganism can be changed by protease digestion
Born of the same parents' osmotic pressure kills this quasi-microorganism, improves the yield and quality of collagen oligopeptide；3）The extracting method can improve collagen
With the touch opportunity of protease, the glycosidic bond of glycoprotein can be cut, makes glycogen degradation, enzymatic hydrolysis rate is improved and collagen oligopeptide obtains
Rate.
Specific embodiment
The present invention program is described further below by embodiment：
Embodiment 1：
The preparation method of fish collagen oligopeptide, including pretreatment, deodorant, enzymatic hydrolysis, the specific steps are that：
1）Pretreatment：Tissue mashing after fish-skin is shredded is 1 by solid-liquid ratio:The oxygen that concentration is 0.13M is added in fish-skin by 4g/mL
It in sodium hydroxide solution, impregnates and constantly stirs, rinsed with circulating water to neutrality after 1.5h, then pressing solid-liquid ratio is 1:2.5g/mL plus
Entering the aqueous isopropanol that concentration is 2%, filtered through gauze after being cleaned repeatedly to neutrality after immersion 15h with distilled water sufficiently drains, then
It is by weight 1:5 deionized water is added into the fish-skin drained, and homogenate, spare, fishskin collagen polypeptide is before preparation, because wherein
Containing many foreign proteins and fat, so to carry out pre-treatment first to remove these foreign proteins, fat etc., substance of this kind can be reduced
Influence to fishskin collagen polypeptide；
2）Deodorant：The arabinose of the active dry yeast and fish-skin weight 0.1% of fish-skin weight 1.3% will be added in homogenate, 43
Then the beta-cyclodextrin of fish-skin weight 8% is added, in the condition that temperature is 60 DEG C, super-pressure is 400MPa in deodorant 20min at DEG C
Lower super-pressure defishying 10min has absorption to make stench substance to get the homogenate of deodorant fish-skin, the loose structure of active dry yeast
With, while active dry yeast contains there are many enzyme, is first converted stench substance to without substance of smelling as of rotten fish with active dry yeast in the step,
Then the macromolecular substances such as stench substance such as aldehyde, ketone recycle the embedding effect of cycloheptaamylose to remove residual by cell accumulation
Yeast fermented flavour and fermentation acid and remaining fishy smell, reach more preferably except raw meat effect, improve the sensory-acceptance of target product；
3）Enzymatic hydrolysis：The pH for adjusting deodorant homogenate is 8.5, is 4.5% addition compound protease by enzyme concentration, adds sodium stearate
And tetrabutylammonium bromide, ultrasonic wave digests 8h under conditions of temperature is 40 DEG C, ultrasonic power is 200W, uses in enzymolysis process
Nanofiltration membrane nanofiltration, nanofiltration liquid are spray-dried to get fish collagen oligopeptide.Different protease act on the site on protein peptide chain
Difference causes composition difference of the Degree of Enzymatic Hydrolysis with polypeptide, which is digested with ultrasonic wave assisted recombination protease, can
It digests Isin glue collagen sufficiently, improves the content of fish collagen oligopeptide in enzymolysis liquid, while the step has enzymatic hydrolysis condition temperature
With reaction process easy to control, it is at low cost the advantages that, in enzymolysis process, have no toxic side effect generation.
The additive amount of sodium stearate is the 2% of compound protease weight in enzymolysis step, and the additive amount of tetrabutylammonium bromide is
The 0.5% of compound protease weight.The special presence of sodium stearate and tetrabutylammonium bromide can not only act on Isin glue collagen
Hydrophobic region, improve dispersibility and stability of the Isin glue collagen in the homogenate of collagen meat, improve Isin glue collagen and albumen
The touch opportunity of enzyme, and then enzymatic hydrolysis rate and collagen peptide yield are improved, and the glycosidic bond of glycoprotein can be cut, glycogen drops
Solution, so that protease quickly identifies that the albumen for the glycogen that is removed is digested, the final yield for improving collagen peptide.
Compound protein is clostridiopetidase A and neutral proteinase, weight ratio 1 in enzymolysis step:4.The step uses clostridiopetidase A
It is digested with neutral proteinase, clostridiopetidase A can exclusively open collagen spy under the premise of not destroying other protein
Some superhelixes, and neutral proteinase can carry out digestion from different amino acid sites, to generate a large amount of collagen
Oligopeptide, while leading to reinfocing effect due to synergistic function between enzyme, the fish collagen oligopeptide segment accordingly generated
Function can further enhance, the content and activity of collagen oligopeptide in the final enzymolysis efficiency for improving fish glue albumen, enzymolysis liquid.
The time interval of nanofiltration is 35min in enzymolysis step, and nanofiltration retaining molecular weight is 1kDa, and the nanofiltration time is
10min.Small molecule product is removed with nanofiltration during the reaction, can reduce the concentration of product in enzymatic hydrolysis system, so that enzymatic hydrolysis
Reaction rate can continue to keep, and greatly shorten enzymolysis time.
D-arabinose in deodorant step in arabinose containing 4.6%.The presence of arabinose can be the dry ferment of activity
Mother provides energy consumption, improves the metabolism amount of alcohol dehydrogenase and aldehyde dehydrogenase, fishy smell substance E-2- decenal is converted to E-2- decene
Pure and mild E-2- decylenic acid, improves the quality of deodorization effect and target product, while being avoided that active dry yeast consumes clam albumen,
Improve target product yield, while in arabinose D-arabinose special presence, keep the collagen of different structure equal
It is very sensitive to pressure, so that the construction of collagen molecules is changed, is denaturalized, so that internal restriction enzyme site exposure,
It is easy to by protease digestion, while fish growth environment keeps itself contained micro organism quantity big, it is mild during proteolysis
Condition be conducive to microbial growth, by product quality and yield have an adverse effect, and D-arabinose cooperate superelevation
Pressure acts on the Premeabilisation of cells pressure that can change microorganism, kills this quasi-microorganism, improves the yield and quality of fish collagen oligopeptide.
Embodiment 2：
The preparation method of fish collagen oligopeptide, including pretreatment, deodorant, enzymatic hydrolysis, the specific steps are that：
1）Pretreatment：Tissue mashing after fish-skin is shredded is 1 by solid-liquid ratio:The oxygen oxygen that concentration is 0.1M is added in fish-skin by 5g/mL
Change in sodium solution, impregnate and constantly stir, rinsed with circulating water to neutrality after 2h, then pressing solid-liquid ratio is 1:Concentration is added in 2g/mL
For 3.5% aqueous isopropanol, filtered through gauze after being cleaned repeatedly to neutrality after 12h with distilled water is impregnated, is sufficiently drained, then by weight
Amount is than being 1:6 deionized water is added into the fish-skin drained, homogenate, spare；
2）Deodorant：The arabinose of the active dry yeast and fish-skin weight 0.2% of fish-skin weight 1% will be added in homogenate, at 40 DEG C
Then the beta-cyclodextrin of fish-skin weight 6% is added, under conditions of temperature is 65 DEG C, super-pressure is 350MPa in lower deodorant 25min
Super-pressure defishying 15min is to get the homogenate of deodorant fish-skin；
3）Enzymatic hydrolysis：Adjust deodorant homogenate pH be 8, by enzyme concentration be 5.0% be added compound protease, add sodium stearate with
Tetrabutylammonium bromide, ultrasonic wave digests 6h under conditions of temperature is 35 DEG C, ultrasonic power is 250W, with receiving in enzymolysis process
Filter membrane nanofiltration, nanofiltration liquid are spray-dried to get fish collagen oligopeptide.
The additive amount of sodium stearate is the 2.3% of compound protease weight, the additive amount of tetrabutylammonium bromide in enzymolysis step
It is the 0.4% of compound protease weight；Compound protein is clostridiopetidase A and neutral proteinase, weight ratio 1:4.5；Nanofiltration when
Between between be divided into 30min, nanofiltration retaining molecular weight is 1kDa, and the nanofiltration time is 15min.
D-arabinose in deodorant step in arabinose containing 4.5%.
Embodiment 3：
The preparation method of fish collagen oligopeptide, including pretreatment, deodorant, enzymatic hydrolysis, the specific steps are that：
1）Pretreatment：Tissue mashing after fish-skin is shredded is 1 by solid-liquid ratio:The oxygen that concentration is 0.08M is added in fish-skin by 6g/mL
It in sodium hydroxide solution, impregnates and constantly stirs, rinsed with circulating water to neutrality after 2.5h, then pressing solid-liquid ratio is 1:1.5g/mL plus
Entering the aqueous isopropanol that concentration is 5%, filtered through gauze after being cleaned repeatedly to neutrality after immersion 10h with distilled water sufficiently drains, then
It is by weight 1:7 deionized water is added into the fish-skin drained, homogenate, spare；
2）Deodorant：The arabinose of the active dry yeast and fish-skin weight 0.1% of fish-skin weight 1.3% will be added in homogenate, 43
Then the beta-cyclodextrin of fish-skin weight 8% is added, in the condition that temperature is 60 DEG C, super-pressure is 400MPa in deodorant 20min at DEG C
Lower super-pressure defishying 10min is to get the homogenate of deodorant fish-skin；
3）Enzymatic hydrolysis：The pH for adjusting deodorant homogenate is 8.5, is 4.5% addition compound protease by enzyme concentration, adds sodium stearate
And tetrabutylammonium bromide, ultrasonic wave digests 8h under conditions of temperature is 40 DEG C, ultrasonic power is 200W, uses in enzymolysis process
Nanofiltration membrane nanofiltration, nanofiltration liquid are spray-dried to get fish collagen oligopeptide.
The additive amount of sodium stearate is the 2% of compound protease weight in enzymolysis step, and the additive amount of tetrabutylammonium bromide is
The 0.5% of compound protease weight；Compound protein is clostridiopetidase A and neutral proteinase, weight ratio 1:4；Between the time of nanofiltration
It is divided into 35min, nanofiltration retaining molecular weight is 1kDa, and the nanofiltration time is 10min.
D-arabinose in deodorant step in arabinose containing 4.6%.
Routine operation in operating procedure of the invention is well known to those skilled in the art, herein without repeating.
Technical solution of the present invention is described in detail in embodiment described above, it should be understood that the above is only
For specific embodiments of the present invention, it is not intended to restrict the invention, all any modifications made in spirit of the invention,
Supplement or similar fashion substitution etc., should all be included in the protection scope of the present invention.
Claims (8)
1. the preparation method of fish collagen oligopeptide, including pretreatment, collagen protein powder preparation, deodorant, enzymatic hydrolysis, filtering, feature
It is：The enzymolysis step is：The pH of deodorant homogenate is adjusted, compound protease, sodium stearate and tetrabutylammonium bromide is added, is surpassed
Sound wave digests, and nanofiltration membrane nanofiltration is used in enzymolysis process, and nanofiltration liquid is spray-dried to get fish collagen oligopeptide.
2. the preparation method of fish collagen oligopeptide according to claim 1, it is characterised in that：The pH of the enzymatic hydrolysis is 7.5-
8.5, enzyme concentration 4.5-5.5%, temperature be 30-40 DEG C, ultrasonic power 200-300W, time 5-8h.
3. the preparation method of fish collagen oligopeptide according to claim 1, it is characterised in that：The addition of the sodium stearate
Amount is the 2-2.6% of compound protease weight, and the additive amount of the tetrabutylammonium bromide is the 0.3- of compound protease weight
0.5%。
4. the preparation method of fish collagen oligopeptide according to claim 1, it is characterised in that：The compound protein is collagen
Enzyme and neutral proteinase, weight ratio 1:4-5.
5. the preparation method of fish collagen oligopeptide according to claim 1, it is characterised in that：The time interval of the nanofiltration
For 25-35min, nanofiltration retaining molecular weight is 1kDa, and the nanofiltration time is 10-20min.
6. the preparation method of fish collagen oligopeptide according to claim 1, it is characterised in that：The pre-treatment step is：
Tissue mashing after fish-skin is shredded is 1 by solid-liquid ratio:The oxygen sodium oxide molybdena that concentration is 0.08-0.13M is added in fish-skin by 4-6g/mL
It in solution, impregnates and constantly stirs, rinsed with circulating water to neutrality after 1.5-2.5h, then pressing solid-liquid ratio is 1:1.5-2.5g/mL
Be added concentration be 2-5% aqueous isopropanol, impregnate 10-15h after cleaned repeatedly to neutrality with distilled water after filtered through gauze, sufficiently
It drains, then is by weight 1:Deionized water is added into the fish-skin drained by 5-7, homogenate, spare.
7. the preparation method of fish collagen oligopeptide according to claim 1, it is characterised in that：The deodorant step is：It will
The active dry yeast of fish-skin weight 0.8-1.3% and the arabinose of fish-skin weight 0.1-0.3% are added in homogenate, at 38-43 DEG C
Lower deodorant 20-30min, then be added fish-skin weight 5-8% beta-cyclodextrin, temperature be 60-70 DEG C, super-pressure 300-
Super-pressure defishying 10-20min is under conditions of 400MPa to get the homogenate of deodorant fish-skin.
8. the preparation method of fish collagen oligopeptide according to claim 7, it is characterised in that：Contain in the arabinose
The D-arabinose of 4.3-4.6%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810473935.XA CN108823269A (en) | 2018-05-17 | 2018-05-17 | The preparation method of fish collagen oligopeptide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810473935.XA CN108823269A (en) | 2018-05-17 | 2018-05-17 | The preparation method of fish collagen oligopeptide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108823269A true CN108823269A (en) | 2018-11-16 |
Family
ID=64148884
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810473935.XA Withdrawn CN108823269A (en) | 2018-05-17 | 2018-05-17 | The preparation method of fish collagen oligopeptide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108823269A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110592053A (en) * | 2019-09-29 | 2019-12-20 | 河南新仰韶生物科技有限公司 | Collagen hydrolysis complex enzyme, protein oligopeptide and preparation method thereof |
| CN111334548A (en) * | 2020-02-21 | 2020-06-26 | 海南美肽生物科技有限公司 | Hydrolyzed collagen compound and preparation method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101601487A (en) * | 2009-07-09 | 2009-12-16 | 山东好当家海洋发展股份有限公司 | A kind of shellfish protein beverage and preparation method thereof |
| JP4400039B2 (en) * | 2002-06-07 | 2010-01-20 | チッソ株式会社 | Method for producing fish scale-derived collagen peptide and calcium apatite |
| CN101875675A (en) * | 2010-06-11 | 2010-11-03 | 江苏惠利隆塑业集团有限公司 | Preparation method of some glucoside |
| CN102690853A (en) * | 2012-03-22 | 2012-09-26 | 浙江省海洋开发研究院 | Preparation method of sleeve-fish skin low-molecular-weight collagen peptide |
| CN102703555A (en) * | 2012-06-08 | 2012-10-03 | 集美大学 | Extraction and preparation method for micromolecule fishskin collagen peptide |
-
2018
- 2018-05-17 CN CN201810473935.XA patent/CN108823269A/en not_active Withdrawn
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4400039B2 (en) * | 2002-06-07 | 2010-01-20 | チッソ株式会社 | Method for producing fish scale-derived collagen peptide and calcium apatite |
| CN101601487A (en) * | 2009-07-09 | 2009-12-16 | 山东好当家海洋发展股份有限公司 | A kind of shellfish protein beverage and preparation method thereof |
| CN101875675A (en) * | 2010-06-11 | 2010-11-03 | 江苏惠利隆塑业集团有限公司 | Preparation method of some glucoside |
| CN102690853A (en) * | 2012-03-22 | 2012-09-26 | 浙江省海洋开发研究院 | Preparation method of sleeve-fish skin low-molecular-weight collagen peptide |
| CN102703555A (en) * | 2012-06-08 | 2012-10-03 | 集美大学 | Extraction and preparation method for micromolecule fishskin collagen peptide |
Non-Patent Citations (1)
| Title |
|---|
| 王燕等: "相转移催化法合成糖苷类化合物的研究进展", 《云南化工》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110592053A (en) * | 2019-09-29 | 2019-12-20 | 河南新仰韶生物科技有限公司 | Collagen hydrolysis complex enzyme, protein oligopeptide and preparation method thereof |
| CN111334548A (en) * | 2020-02-21 | 2020-06-26 | 海南美肽生物科技有限公司 | Hydrolyzed collagen compound and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108823270A (en) | A kind of extracting method of oyster peptide | |
| CN102199646B (en) | Method for preparing collagen peptide with fish skin | |
| CN102690853B (en) | Preparation method of sleeve-fish skin low-molecular-weight collagen peptide | |
| CN102676619B (en) | Comprehensive utilization process for marine fish skins | |
| CN108823269A (en) | The preparation method of fish collagen oligopeptide | |
| CN108728507A (en) | The preparation method of fishskin collagen polypeptide | |
| US20040253678A1 (en) | Novel collagen production method | |
| CN1769424A (en) | Bacillus strain and its uses | |
| CN101869169B (en) | Method for preparing fish oligopeptide from gurry by combining fermentation and membrane technology | |
| CN110495611A (en) | A kind of technique improving sea cucumber nutritional health effect | |
| CN108148131A (en) | The method that collagen is extracted from squid skin | |
| CN110564802A (en) | Extraction method of yak achilles tendon bone collagen | |
| CN106591408A (en) | Method for preparing trepang collagen peptide by adopting microbiological fermentation method | |
| CN109486887A (en) | It is a kind of ultrasound and enzymatic hydrolysis coordinate system for oyster polypeptide method | |
| JP3481328B2 (en) | Method for producing fermented liquid for adding food or beverage and fermented liquid for adding food or beverage | |
| CN108822206A (en) | A kind of biologically active cod collagen peptide | |
| CN112715815A (en) | Cod micromolecular collagen peptide solid beverage | |
| JP4180108B1 (en) | Method for producing fermentation product and fermentation product | |
| US7429561B2 (en) | Method for stimulating cell growth using sponge protein hydrolysates | |
| CN109097426A (en) | A kind of preparation method of giant salamander protein peptides | |
| CN107815480A (en) | With the method for catfish fish guts protease extraction collagen | |
| CN108546727A (en) | The method that collagen polypeptide is extracted from fish scale | |
| CN110818791B (en) | Preparation method of fish collagen | |
| CN108707641A (en) | The method that collagen polypeptide is extracted from fish scale | |
| CN105624242A (en) | Method for preparing active peptide through high-temperature fermentation of aquatic protein |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20181116 |