CN108823130B - Biocontrol strain GacAI of two-component regulation and control system for inhibiting plant pathogenic bacteria GacS/GacA and application - Google Patents
Biocontrol strain GacAI of two-component regulation and control system for inhibiting plant pathogenic bacteria GacS/GacA and application Download PDFInfo
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Abstract
The invention provides a biological control strain GacAI of a two-component regulation and control system for inhibiting plant pathogenic bacteria GacS/GacA and application thereof, wherein the biological control strain is classified and named as Bacillus subtilis GacAI, and a preservation unit comprises the following components: china general microbiological culture Collection center (CGMCC for short), preservation date: 6 and 11 days in 2018, and the accession number is: CGMCC No.15939, the bacillus subtilis GacAI secondary metabolite can effectively inhibit GacS/GacA system expression in plant pathogenic bacteria, thereby influencing the expression of pathogenic genes such as pectate lyase and other coding genes, the suspending agent prepared by taking the bacillus subtilis GacAI as an effective component can effectively prevent and treat Chinese cabbage soft rot, and the prevention and treatment effect on the Chinese cabbage soft rot reaches 84.1%.
Description
The technical field is as follows:
the invention relates to the technical field of biology, in particular to a biocontrol strain GacAI for a two-component regulation and control system for inhibiting bacteria GacS/GacA and application thereof.
Technical background:
the ability of bacteria to survive in a particular habitat requires the coordination of the expression of appropriate genes to cope with the change in the environment. In order to cope with the complicated external environment, various induction regulation systems such as GacS/GacA two-component regulation system (TCS) have been developed for bacteria, which monitor the external environment change and make an appropriate adaptive response to the environmental change. The GacS/GacA system consists of a sensor kinase (sensor kinase) GacS and a corresponding response regulator (response regulator) GacA. Autophosphorylation of the kinase GacS is induced by interaction with a signaling molecule. Phosphorylated GacS transfers phosphate groups to the corresponding response regulator GacA, and activated GacA regulates the expression of the downstream target gene (Heeb, S., and D.Haas. (2001). Regulation roles of the GacS/GacA two-component system in plant-associated and other Gram-novel bacteria. mol.plant-microorganism interaction.14: 1351-1363.). At present, the regulation and control system is one of the bacterial pathogenicity research hotspots which are paid more attention by researchers.
The expression of genes involved in pathogenicity of various gram-negative pathogenic bacteria in plants is strictly regulated by the GacS/GacA system. Such as: pectobacterium carotovorum (Pectobacterium carotovorum subsp. carotovorum, Pcc), which causes soft rot in a variety of plants, such as potatoes, cabbage, carrots, etc., synthesizes a number of hydrolytic enzymes upon infestation of a host plant, such as: pectinase, polygalacturonase, pectate lyase, etc., to destroy plant cell wall and cause soft rot. The production of these extracellular enzymes that disrupt the host cell wall and other virulence factors that interact with the host plant are tightly regulated by the GacS/GacA system (Cui, Y., A. Chatterjee, and A. K. Chatterjee. (2001). Effects Soft-component system comprises GacA and GacS of Erwinia carotovorubsp. carotovora on the production of global regulatory rsmB RNA, extracellular enzymes, and Harpinec. mol.plant-Microbe interaction.14: 516. 526.). In addition to Pectinopsis carotovora, the GacS/GacA system affects pathogenic expression in other plant pathogenic bacteria. Such as: xylem fastidiosa (Xylella fastidiosa), which is regulated by the GacS/GacA system with pathogenic factors such as adhesion and biofilm formation, is significantly less pathogenic than mutants of the GacS/GacA system (Shi, x.y., c.k.dumenyo, r.hernandez-Martinez, et al (2009) mutation of regulation pathway in Xylella fastidiosa: genes and viruses controlled bygaca.appl.environ.75: 2275. 2283.). The colonization of host plants by Pseudomonas syringae (Pseudomonas syringae) and the production of various virulence factors also require the involvement of the GacS/GacA system (Chatterjee, A., Y. Cui, H.Yang, et al (2003). GacA, the resA stress regulator of a two-component system, acts as a master regulator in Pseudomonas syringae pv. a. b. a. The GacS/GacA system in the plant pathogenic bacterium Dickeyadantii plays an important role in the pathogenic process of this bacterium by affecting the expression of pectin lyase, protease, cellulase and the three-type secretory system (Yang, S.Q., Q.Peng., Q.Zhang, et al (2008). Dynamic regulation of GacA in type III section, plasmid gene expression, plasmid format, and pathology of Dickya dantii (Erwinia chrysogeni 3937. mol.plant-Microbe interaction.21: 133. 142.). Similarly, deletion of the GacS/GacA two-component regulation system in xanthomonas oryzae pv. oryzae significantly affects the motility of the strain, and the bacterial motility is closely related to the pathogenicity of the strain (xu Jing Sheng, Wu Tong, He Cheng Yang (2010). the two-component regulation system of xanthomonas oryzae responds to the regulator gene GacAxooThe functional identification of (1) in the plant pathology report (40) (3) 282-289). The above reports indicate that the GacS/GacA system is widely involved in the regulation of pathogenicity of pathogenic bacteria.
Certain environmental factors, tricarboxylic acid cycle intermediate metabolites, azithromycin and other antibiotics can influence the GacS/GacA two-component regulation system. If the pH value is lower than 5.5, the BarA/UvrY system which is the same as the GacS/GacA system in the strain Escherichia coli is in a closed state; azithromycin (azithromycin) is a macrolide antibiotic that is widely used in clinical applications. Although Azithromycin does not affect the growth of human opportunistic pathogen p. aeruginosa in clinically used concentrations, Azithromycin can treat infections caused by p. aeruginosa mainly by inhibiting the translation of the gacA gene and the transcription of small RNA molecules RsmY and RsmZ (Perez-Martinez, i.and d. haas. (2011.) of the gacA-dependent small RNA RNAs RsmY and RsmZ in pseudomonas aeruginosa.
At present, the prevention and the treatment of crop bacterial diseases are mainly based on the use of traditional antibiotics. The traditional antibiotic action mechanism is generally to directly kill pathogenic bacteria, and the large amount of use of the traditional antibiotic also causes the environmental safety problems such as the improvement of the drug resistance of pathogenic bacteria and the like. The GacS/GacA two-component regulation system plays a key role in the pathogenic process of various plant pathogenic bacteria, and the deletion of the system does not influence the growth of pathogenic bacteria. Therefore, the screening of secondary metabolite resources for inhibiting the GacS/GacA dual-factor regulation system has wide application prospect when being specially used for preventing and treating the bacterial diseases.
The invention content is as follows:
the invention aims to provide a bacillus subtilis GacAI and application thereof in preventing and treating bacterial diseases.
The above purpose is realized by the following technical scheme:
a Bacillus subtilis is classified and named as Bacillus subtilis GacAI, and a preservation unit: china general microbiological culture Collection center (CGMCC for short), preservation date: 6 and 11 days in 2018, and the accession number is: CGMCC No. 15939.
The secondary metabolite secreted by the bacillus subtilis GacAI CGMCC No.15939 can inhibit the expression of a GacS/GacA system in pathogenic bacteria, thereby influencing the pathogenicity of the pathogenic bacteria.
The bacillus subtilis GacAI fermentation liquor is prepared into a biocontrol microbial inoculum for preventing and treating the cabbage soft rot.
The biocontrol microbial inoculum is a suspending agent and consists of bacillus subtilis GacAI fermentation liquor, N-dodecyl-beta-aminopropionic acid sodium, hydroxymethyl cellulose, acid hydrolyzed casein, sodium benzene sulfonate sulfate and peptidoglycan.
The suspending agent consists of the following components:
1000mL of bacillus subtilis GacAI fermentation broth
20g of sodium N-dodecyl-beta-aminopropionate
Hydroxymethyl cellulose 3g
Acid hydrolyzed casein 1g
Sodium benzenesulfonate sulfate 2g
Peptidoglycan 0.3g
Bacillus subtilis GacAI the spore content in the fermentation liquid is not less than 55 × 108CFU/mL。
The particle size of each component in the suspending agent is not more than 5 mu m.
The invention has the beneficial effects that: according to the invention, the bacillus subtilis GacAI secondary metabolite can effectively inhibit the expression of a GacS/GacA system in plant pathogenic bacteria, so that the expression of pathogenic genes such as pectate lyase and other coding genes is influenced. The suspending agent prepared by taking the bacillus subtilis GacAI as an effective component can effectively prevent and treat the soft rot of the Chinese cabbage, and the prevention and treatment effect on the soft rot of the Chinese cabbage reaches 84.1 percent.
Description of the drawings:
FIG. 1 shows that Bacillus subtilis GacAI secondary metabolites inhibit the expression of the small RNA coding gene rsmB; FIG. 2 shows that Bacillus subtilis GacAI secondary metabolite inhibits generation of signal molecules of cabbage soft rot pathogen quorum sensing system; FIG. 3 shows that Bacillus subtilis GacAI secondary metabolite inhibits pathogenicity of cabbage soft rot pathogen Pcc Z3-3.
The specific implementation mode is as follows:
in order to clarify the present invention, the following detailed description will explain the technical solution of the present invention by using specific examples, but the present invention is not limited thereto.
Example 1
The bacillus subtilis GacAI secondary metabolite inhibits the expression of a small RNA encoding gene rsmB in phytopathogen Pectobacterium carotovorum (Pcc) Z3-3.
The GacS/GacA system is regulating the expression of the small RNA coding gene rsmB. After PCR amplification of the promoter region of the rsmB gene, it was cloned into a promoter activity detecting plasmid pRG970Gm, thereby obtaining a transcriptional reporter construct (rsmB-lacZ) of rsmB, the expression of which was expressed by the GacS/GacA system. After 1 week incubation of the Bacillus subtilis GacAI at 28 ℃ the supernatant was extracted with an equal volume of ethyl acetate and the extract was re-suspended in 0.1mL of dimethyl sulfoxide (DMSO) after rotary evaporation. mu.L of the extract was added to 2mL of Pcc Z3-3(p970Gm-rsmBp) and its gacA gene mutant (p970Gm-rsmBp) (OD600 ═ 0.1), and after 12h of culture, the β -galactosidase activity in the reporter was detected.
Referring to fig. 1, the bacillus subtilis GacAI extracting solution can obviously inhibit the expression of a small RNA coding gene rsmB in pathogenic bacteria Z3-3, and the inhibition rate reaches 80%. On the other hand, the addition of GacAI extract to the Z3-3gacA mutant did not lower the expression of rsmB gene more than that of the wild type strain Z3-3, indicating that GacAI extract affects the expression of rsmB gene by GacS/GacA system.
Example 2
The bacillus subtilis GacAI secondary metabolite inhibits quorum sensing signal molecules in phytopathogen Pcc Z3-3.
After overnight culture of the strain Z3-3 in LB medium, the ratio of 1: 1000 dilutions were inoculated into LB medium containing varying concentrations of GacAI secondary metabolite. After incubation at 28 ℃ for 12h, the supernatant was extracted with an equal volume of ethyl acetate and the extract was rotary evaporated to dryness and redissolved in 100 μ L of methanol. mu.L of the sample was dropped onto detection plates of quorum sensing reporter strains Agrobacterium NTL4(pZLR4) and X-gal, and after overnight incubation, the results were recorded.
Referring to fig. 2, the Z3-3 quorum sensing system signal molecule was detected by using quorum system reporter strain a. tumefaciens NTL4(pZLR4), and it was found that the generation of Z3-3 quorum sensing system signal molecule could be significantly reduced by adding 10 μ L GacAI extract with a concentration of 30 μ g/mL to the detection plate; on the other hand, 40. mu.L of GacAI extract with a concentration of 30. mu.g/mL was added to almost completely inhibit the production of signal molecules of quorum sensing system of strain Z3-3.
Example 3
The bacillus subtilis GacAI secondary metabolite inhibits the pathogenicity of plant pathogenic bacteria Pcc Z3-3.
To examine the effect of the GacAI secondary metabolites on Pcc Z3-3 pathogenicity, strain Z3-3 was cultured overnight and then mixed as follows: 1000 parts of the culture were transferred to a fresh LB medium and cultured at 28 ℃ until OD600 became 1.0. And (3) uniformly mixing 5 mu L of bacterial liquid with GacAI secondary metabolites with different concentrations, inoculating the mixture to the Chinese cabbage, performing moisture-preserving culture at 28 ℃ for 24 hours, and measuring the size of the lesion spots.
Referring to fig. 3, compared with the control, the wild fungi can obviously cause the leaf of the Chinese cabbage to rot; after 10 mu L of GacAI extract with the concentration of 30 mu g/mL and the wild type strain Z3-3 are mixed and inoculated to the Chinese cabbage, the pathogenicity of the strain Z3-3 can be obviously reduced, and after 40 mu L of GacAI extract with the concentration of 30 mu g/mL and the wild type strain Z3-3 are mixed and inoculated to the Chinese cabbage, the pathogenicity of the strain Z3-3 can be completely inhibited. These results indicate that secondary metabolites produced by strain GacAI can affect the pathogenicity of strain Z3-3.
Example 4
Preparation of the suspension
Preparing the bacillus subtilis GacAI fermentation liquor, wherein the spore content is not lower than 55 × 10 after the fermentation is finished8CFU/mL, suspension formulations were prepared in the following proportions.
1000mL of bacillus subtilis GacAI fermentation broth
20g of sodium N-dodecyl-beta-aminopropionate
Hydroxymethyl cellulose 3g
Acid hydrolyzed casein 1g
Sodium benzenesulfonate sulfate 2g
Peptidoglycan 0.3g
The suspending agent contains secondary metabolite produced by Bacillus subtilis GacAI as effective component, and spore formation can prolong the effect of the preparation. N-dodecyl-beta-aminopropionic acid sodium in the suspending agent is taken as an auxiliary agent and a dispersing agent; hydroxymethyl cellulose is thickener and is a protective agent of effective component; the particle size of each component in the suspending agent is not more than 5 mu m.
Example 5
Test of field control effect of suspending agent on Chinese cabbage soft rot
Test site: guangxi Nanning, Guangxi university experiment base. Chinese cabbage variety: qingza No. three. 3 treatments are set in the test, wherein the treatment 1 is GacAI suspending agent and 100 times of diluent; treatment 2 is 20% thiabendazole copper, 500 times of diluent; treatment 3 was a clear water control with 3 replicates per treatment. The treatment method comprises the following steps: and (4) diluting and spraying. The administration time is as follows: 10 days in 12 months in 2017, 30 days in 12 months in 2017, and 20 days in 1 month in 2018, with the interval time of 20 days. Investigation of events: year 2018, month 3 and day 25.
The test results (see table 1) show that the suspending agent has a good effect of preventing and treating the soft rot of the Chinese cabbage in the Guangxi university test base, is significantly different from a control medicament (p is less than 0.01), and has an effect of preventing and treating the soft rot of the Chinese cabbage of 84.1%.
Table 1 shows comparative data of the field control effect of the suspension concentrate of the present invention on soft rot of chinese cabbage in the university of Guangxi.
| Treatment of | The incidence of disease% | Control effect% |
| The suspending agent of the invention | 13.3A | 84.1 |
| 20% Thiodiazole copper | 20.9B | 74.9 |
| Clean water | 83.4C | - |
Claims (5)
1. A biocontrol strain GacAI for inhibiting a plant pathogenic bacterium GacS/GacA two-component regulation system is characterized in that the biocontrol strain is named as bacillus subtilis (Bacillus subtilis)Bacillus subtilis) GacAI, depository: china general microbiological culture Collection center (CGMCC for short), preservation date: 6 and 11 days in 2018, and the accession number is: CGMCCNo.15939.
2. The application of the biocontrol strain GacAI for inhibiting the plant pathogenic bacteria GacS/GacA two-component regulation system according to claim 1, wherein a biocontrol microbial inoculum is prepared from a Bacillus subtilis GacAI fermentation broth to prevent and control the soft rot of Chinese cabbage.
3. The use of the GacS/GacA two-component control system biocontrol strain GacA i for inhibiting plant pathogenic bacteria of claim 2 wherein the bacillus subtilis GacA i fermentation broth has a spore content of no less than 55 × 108CFU/mL。
4. The application of the gacS/GacA two-component control system biocontrol strain GacAI for inhibiting plant pathogenic bacteria as claimed in claim 2 or 3, wherein the biocontrol microbial inoculum is a suspending agent, and is prepared from bacillus subtilis GacAI fermentation liquor, N-dodecyl-beta-amino sodium propionate, hydroxymethyl cellulose, acid hydrolyzed casein, sodium benzenesulfonate sulfate and peptidoglycan according to the following mixture ratio:
5. the use of a GacS/GacA two-component control system biocontrol strain GacA i for inhibiting phytopathogenic bacteria according to claim 4 wherein the particle size of each component of the suspension is not greater than 5 μm.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5548874B2 (en) * | 2010-03-31 | 2014-07-16 | 富山県 | Cancer immunosuppression releasing agent and cancer immunotherapy composition |
| CN105219681A (en) * | 2015-11-04 | 2016-01-06 | 湖北工程学院 | A kind of bacillus amyloliquefaciens Bacillus amyloliquefaciens D2WM and preparation method and application |
| CN105296387A (en) * | 2015-10-28 | 2016-02-03 | 哈尔滨医科大学 | Streptomyces cyaneofuscatus strain and anti-cancer active metabolite and application thereof |
| CN108118019A (en) * | 2018-03-09 | 2018-06-05 | 易虹 | Bacillus subtilis strain and its application |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5548874B2 (en) * | 2010-03-31 | 2014-07-16 | 富山県 | Cancer immunosuppression releasing agent and cancer immunotherapy composition |
| CN105296387A (en) * | 2015-10-28 | 2016-02-03 | 哈尔滨医科大学 | Streptomyces cyaneofuscatus strain and anti-cancer active metabolite and application thereof |
| CN105219681A (en) * | 2015-11-04 | 2016-01-06 | 湖北工程学院 | A kind of bacillus amyloliquefaciens Bacillus amyloliquefaciens D2WM and preparation method and application |
| CN108118019A (en) * | 2018-03-09 | 2018-06-05 | 易虹 | Bacillus subtilis strain and its application |
Non-Patent Citations (3)
| Title |
|---|
| An Extract Produced by Bacillus sp. BR3 Influences the Function of the GacS/GacA Two-Component System in Pseudomonas syringae pv. tomato DC3000;Bo Zhang 等;《Frontiers in Microbiology》;20190911;第1-12页 * |
| GacS/GacA双因子调控系统和群体感应系统在生防荧光假单胞杆菌2P24中的作用;魏海雷;《万方》;20050714;全文 * |
| 白菜根腐病的特征及其防治措施;贾水娜 等;《北京农业》;20130925;第100页 * |
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