CN108823117A - One plant of Paracoccus denitrificans bacterial strain, cultural method and its application with sulphur iron co-substrate autotrophic denitrification function - Google Patents

One plant of Paracoccus denitrificans bacterial strain, cultural method and its application with sulphur iron co-substrate autotrophic denitrification function Download PDF

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CN108823117A
CN108823117A CN201810533202.0A CN201810533202A CN108823117A CN 108823117 A CN108823117 A CN 108823117A CN 201810533202 A CN201810533202 A CN 201810533202A CN 108823117 A CN108823117 A CN 108823117A
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paracoccus denitrificans
bacterial strain
autotrophic denitrification
sulphur iron
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CN108823117B (en
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张玉
葛小帅
孙超越
苏志强
周翔
孙利剑
赵睿智
周集体
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Dalian University of Technology
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    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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    • C02F2101/00—Nature of the contaminant
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    • C02F2101/16—Nitrogen compounds, e.g. ammonia
    • C02F2101/163—Nitrates
    • C—CHEMISTRY; METALLURGY
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    • C02F2101/00—Nature of the contaminant
    • C02F2101/10—Inorganic compounds
    • C02F2101/16—Nitrogen compounds, e.g. ammonia
    • C02F2101/166—Nitrites

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Abstract

The invention belongs to field of environmental biotechnology, are related to one plant of Paracoccus denitrificans bacterial strain, cultural method and its application with sulphur iron co-substrate autotrophic denitrification function.The bacterium is Paracoccus denitrificans ZGL1, is under anaerobic nitrogen by nitrate in waste water or nitrate reductase as electron donor by pyrite or ferrous sulfide.The bacterium carries out denitrogenation of waste water by sulphur iron co-substrate autotrophy process, and low energy consumption, simple process, sludge quantity are few, has biggish application potential in denitrogenation of waste water field.

Description

One plant has the Paracoccus denitrificans bacterial strain of sulphur iron co-substrate autotrophic denitrification function, training Support method and its application
Technical field
The invention belongs to field of environmental biotechnology, are related to one plant of taking off with sulphur iron co-substrate autotrophic denitrification function Nitrogen pair meningitidis strains, cultural method and its application in denitrogenation of waste water treatment process.
Background technique
In recent years, the discharge amount of industrial wastewater and sanitary sewage is gradually with the industrial development and social progress in China Increase, although China uses the sewage treatment policy being increasingly stringenter and measure, water pollution problems are still serious.? It announces within 2013《China Environmental State Bulletin》It shows, the ammonia nitrogen discharge in the whole country in industrial wastewater and municipal wastewater Amount has reached 253.6 ten thousand tons, and in visible a period of time, the discharge amount of China's ammonia nitrogen pollutant is still quite huge.
It is well known that a large amount of inorganic chemical nitrogen can cause water eutrophication after entering water body, original water body is destroyed The ecological balance.Furthermore the ammonia nitrogen entered in water body is easily converted into nitrate nitrogen and nitrite nitrogen, so Water compound The removing problem of (ammoniacal nitrogen, nitrate nitrogen and nitrite nitrogen) is the hot spot paid close attention in recent years.This pollutant is widely present in lake Pool, reservoir, ocean etc. are stored in water body, and serious ecological problem is brought.2016《China Environmental State Bulletin》It has been shown that, ground Be lauched water source monitoring section (point) 334, have 284 whole years up to standard, account for 85.0%, main exceeded index be manganese, iron and Ammonia nitrogen.In 24 surface water elementary items of reservoir area of Three Gorges the Changjiang river fork monitoring, 9 indexs appearance are exceeded, and exceeding standard rate is most Big is that total nitrogen reaches 89.5%.Total nitrogen exceeded phenomena such as causing water eutrophication, generating wawter bloom, red tide in water body, By serious pollution receiving water body and it is detrimental to health.Therefore, the improvement of nitrogenous effluent is in current China's water prevention and cure of pollution Significant problem.
Improvement for nitrogenous effluent, bio-denitrification technology is since it is fast and safely and by favor.Denitrification denitrogenation work Skill is considered as the removing water process mode the most cost-effective of NO3-N and NO2-N in water.Denitrification process is divided into Heterotrophic denitrification and autotrophic denitrification.Most of denitrifying bacteria belongs to heterotroph, needs to be additionally provided organic matter as electronics Donor.But by aoxidizing inorganic matter (H2, S2-, S2O3 2-, Fe, Fe2+And NH4 +Deng, Zhang Yanhao, Zhong Fohua, summer cleaning politics, economics, organization, and ideology, at water Reason technology, 2009,35 (5):75-78,104； Sahinkaya E et al,Water Research,2014,60(9):210- 217；Larese-Casanova P, Geochimica et Cosmochimica Acta,2010,74(13):3721- 3734；) be equally prevalent in nature to restore the autotrophic denitrification bacterium of nitrate.Compared to heterotrophic denitrification, certainly Support denitrification have the advantages that not need to be additionally provided organic matter, sludge quantity it is few and without secondary pollution.
Reduced sulfur compounds are in lithosphere and hydrosphere mainly with troilite such as pyrite, marcasite and magnetic iron ore Compound form exists, and these troilite compound especially pyrite are that sulphur and iron are main in biogeochemical cycle Storage form, and ferrous sulfide is also easy to get.It can be seen that occurring using sulphur iron autotrophic denitrification is probably synchronous In same system, therefore it is useless in autotrophic denitrification removing to find one plant of bacterium with sulphur iron co-substrate autotrophic denitrification function It is had practical application value in terms of NO3-N and NO2-N in water.
Summary of the invention
It is an object of the invention to aiming at the problem that NO3-N and NO2-N in waste water pollutes, providing one plant has sulphur The bacterium of iron co-substrate autotrophic denitrification function removes industrial wastewater, life under the conditions of no additional carbon or insufficient carbon source NO3-N and NO2-N in sewage and underground water living.
Technical solution of the present invention：
One plant of Paracoccus denitrificans bacterial strain with sulphur iron co-substrate autotrophic denitrification function, the Paracoccus denitrificans bacterial strain are Paracoccus denitrificans ZGL1, deposit number：CCTCC M 2012158, preservation place：Chinese Typical Representative culture Collection, preservation time：On May 7th, 2012, preservation address：Wuhan, China, Wuhan University.
The cultural method of one plant of Paracoccus denitrificans bacterial strain with sulphur iron co-substrate autotrophic denitrification function, steps are as follows：
Under anaerobic, Paracoccus denitrificans ZGL1 is inoculated in culture medium, condition of culture is：It is 7.0,30 DEG C of pH, permanent Temperature stands Anaerobic culturel；
Sulphur iron co-substrate autotrophic denitrification nutrient media components：2~10mmol/L of nitrate nitrogen, pyrite or ferrous sulfide 20~500mmol/L, KH2PO414.7mmol/L NaHCO330mmol/L, NH4Cl 18.7 mmol/L, MgCl2·6H2O 3.25mmol/L CaCl2·2H2O 0.05mmol/L passes through sterilization treatment before culture medium use.
The purposes of one plant of Paracoccus denitrificans bacterial strain with sulphur iron co-substrate autotrophic denitrification function, steps are as follows：
Under anaerobic, which removes nitrate for waste water sulphur iron co-substrate autotrophic denitrification Or nitrite：Using pyrite or ferrous sulfide as electron donor by nitrate in waste water or nitrate reductase as nitrogen.
Can use pyrite or ferrous sulfide the beneficial effects of the invention are as follows the bacterial strain will be in waste water as electron donor Nitrate or nitrate reductase are nitrogen.It is carried out under anaerobic by the bacterium by sulphur iron co-substrate autotrophy process useless Denitrogenation of water, low energy consumption, simple process, sludge quantity are few.Industrial wastewater and the carbon source for being particularly suitable for no additional carbon are insufficient It is lauched the removal of middle NO3-N and NO2-N, cost is relatively low, simple process, there is stronger practical value.
Specific embodiment
Below in conjunction with technical solution, a specific embodiment of the invention is further illustrated.
Embodiment 1：Bacterial strain of the present invention removes nitrate in waste water by electron donor of pyrite under anaerobic
Denitrogenation culture medium：KH2PO414.7mmol/L NaHCO330mmol/L, NH4Cl 18.7mmol/L, MgCl2· 6H2O 3.25mmol/L, CaCl2·2H2O 0.05mmol/L, pyrite 500mmol/L adjust pH 7.0~7.2, culture medium Pass through sterilization treatment before use.
Using denitrogenation culture medium, the bacterial strain ZGL1 for being inoculated with that dry weight is 0.8g/L under anaerobic is cultivated at 30 DEG C, The variation of nitrate, nitrite concentration is measured by sampling in separated in time, the results are shown in Table 1.From table 1 it follows that detesting Under the conditions of oxygen, using pyrite as electron donor, nitrate can be degradable, by the culture of 30h, 100% nitric acid in system Salt is removed.Nitrite pilot process has accumulation, is fully converted to nitrogen when reacting 102h.
1 bacterial strain ZGL1 of table removes nitrate in waste water by electron donor of pyrite
Embodiment 2：Bacterial strain of the present invention removes nitrate in waste water by electron donor of ferrous sulfide under anaerobic
Denitrogenation culture medium：KH2PO414.7mmol/L NaHCO330mmol/L, NH4Cl 18.7mmol/L, MgCl2· 6H2O 3.25mmol/L, CaCl2·2H2O 0.05mmol/L, ferrous sulfide 500mmol/L adjust pH 7.0~7.2, culture Pass through sterilization treatment before base use
Using denitrogenation culture medium, the bacterial strain ZGL1 for being inoculated with that dry weight is 0.8g/L under anaerobic is cultivated at 30 DEG C, The variation of nitrate, nitrite concentration is measured by sampling in separated in time, the results are shown in Table 2.From Table 2, it can be seen that detesting Under the conditions of oxygen, using ferrous sulfide as electron donor, nitrate can be degradable, by culture for 24 hours, 100% nitre in system Hydrochlorate is removed.Nitrite pilot process has accumulation, is fully converted to nitrogen after reacting 120h.
2 bacterial strain ZGL1 of table removes nitrate in waste water by electron donor of ferrous sulfide

Claims (3)

1. one plant of Paracoccus denitrificans bacterial strain with sulphur iron co-substrate autotrophic denitrification function, which is characterized in that the denitrogenation Secondary meningitidis strains are Paracoccus denitrificans ZGL1, deposit number：CCTCC M 2012158, preservation place：In State's Type Tissue Collection, preservation time：On May 7th, 2012.
2. the cultural method of one plant of Paracoccus denitrificans bacterial strain with sulphur iron co-substrate autotrophic denitrification function, which is characterized in that Steps are as follows：
Under anaerobic, Paracoccus denitrificans ZGL1 is inoculated in culture medium, condition of culture is：7.0,30 DEG C of pH, constant temperature is quiet Set Anaerobic culturel；
Sulphur iron co-substrate autotrophic denitrification nutrient media components：2~10mmol/L of nitrate nitrogen, pyrite or ferrous sulfide 20~ 500mmol/L, KH2PO414.7mmol/L NaHCO330mmol/L, NH4Cl 18.7mmol/L, MgCl2·6H2O 3.25mmol/L CaCl2·2H2O 0.05mmol/L passes through sterilization treatment before culture medium use.
3. the purposes of one plant of Paracoccus denitrificans bacterial strain with sulphur iron co-substrate autotrophic denitrification function, which is characterized in that step It is as follows：
Under anaerobic, the Paracoccus denitrificans bacterial strain is for waste water sulphur iron co-substrate autotrophic denitrification removing nitrate or nitrous Hydrochlorate：Using pyrite or ferrous sulfide as electron donor by nitrate in waste water or nitrate reductase as nitrogen.
CN201810533202.0A 2018-05-21 2018-05-21 Paracoccus denitrificans strain with sulfur-iron co-matrix autotrophic denitrification function, culture method and application thereof Active CN108823117B (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110452846A (en) * 2019-08-16 2019-11-15 鹭滨环保科技（上海）股份有限公司 A kind of Paracoccus denitrificans and its method with mineralising bed combination progress biological denitrificaion
CN114686401A (en) * 2022-04-14 2022-07-01 华南理工大学 Biological FeS nano particle reinforced microbial agent, preparation method thereof and method for applying biological FeS nano particle reinforced microbial agent to denitrification
CN115820466A (en) * 2022-09-21 2023-03-21 恒臻(无锡)生物科技有限公司 Sulfur autotrophic denitrification strain, bacterial preparation and application thereof

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110452846A (en) * 2019-08-16 2019-11-15 鹭滨环保科技（上海）股份有限公司 A kind of Paracoccus denitrificans and its method with mineralising bed combination progress biological denitrificaion
CN110452846B (en) * 2019-08-16 2022-07-12 鹭滨环保科技（上海）股份有限公司 Paracoccus denitrificans and biological denitrification method by combining paracoccus denitrificans with mineralization bed
CN114686401A (en) * 2022-04-14 2022-07-01 华南理工大学 Biological FeS nano particle reinforced microbial agent, preparation method thereof and method for applying biological FeS nano particle reinforced microbial agent to denitrification
CN115820466A (en) * 2022-09-21 2023-03-21 恒臻(无锡)生物科技有限公司 Sulfur autotrophic denitrification strain, bacterial preparation and application thereof
CN115820466B (en) * 2022-09-21 2023-11-07 恒臻(无锡)生物科技有限公司 Sulfur autotrophic denitrification strain, bacterial preparation and application thereof

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