CN108815187A - A kind of preparation and its application method comprising person joint's liquid excretion body - Google Patents
A kind of preparation and its application method comprising person joint's liquid excretion body Download PDFInfo
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- CN108815187A CN108815187A CN201810803915.4A CN201810803915A CN108815187A CN 108815187 A CN108815187 A CN 108815187A CN 201810803915 A CN201810803915 A CN 201810803915A CN 108815187 A CN108815187 A CN 108815187A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/24—Mucus; Mucous glands; Bursa; Synovial fluid; Arthral fluid; Excreta; Spinal fluid
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
- A61K31/19—Carboxylic acids, e.g. valproic acid
- A61K31/195—Carboxylic acids, e.g. valproic acid having an amino group
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
- A61K31/19—Carboxylic acids, e.g. valproic acid
- A61K31/195—Carboxylic acids, e.g. valproic acid having an amino group
- A61K31/197—Carboxylic acids, e.g. valproic acid having an amino group the amino and the carboxyl groups being attached to the same acyclic carbon chain, e.g. gamma-aminobutyric acid [GABA], beta-alanine, epsilon-aminocaproic acid, pantothenic acid
- A61K31/198—Alpha-aminoacids, e.g. alanine, edetic acids [EDTA]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
- A61K31/365—Lactones
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
- A61K31/365—Lactones
- A61K31/375—Ascorbic acid, i.e. vitamin C; Salts thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/38—Albumins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P19/00—Drugs for skeletal disorders
- A61P19/02—Drugs for skeletal disorders for joint disorders, e.g. arthritis, arthrosis
Abstract
The invention discloses a kind of preparations comprising person joint's liquid excretion body, including following component：Person joint's liquid excretion body 20-30%, polyglycol solution 15-18%, human serum albumin 7-12%, methionine 6.5-7.5%, propylene carbonate 5.3-6.7%, Catergen .1-3.8%, tranexamic acid 1.35-2.28%, solvent 4.87-5.86%, phosphide acylsarcosine compound 5.66-7.32%, deionized water are surplus；The invention discloses a kind of application methods of preparation comprising person joint's liquid excretion body, include the following steps：The separation and Extraction of person joint's liquid excretion body, prepare preparation, degerming, recovery freeze and inject use；Formula design of the invention is more reasonable, obtains person joint's liquid excretion body by separation and Extraction, has good curative effect for treatment arthropathy, compares drug or operative treatment, and the use of the excretion body preparation is more safe, reduces the wound caused by patient body；And the excretion body preparation can be with long-term preservation after being made, and excretion body preparation is also very convenient when in use, and therapeutic effect is good, is conducive to promote and apply.
Description
Technical field
The present invention relates to field of biomedicine technology, specially a kind of preparation comprising person joint's liquid excretion body and its use
Method.
Background technique
Excretion body refers to that the small film bubble (30-150nm) for containing complicated RNA and protein refers in particular to diameter and exist now
The plate-like vesica of 40-100nm, nineteen eighty-three, excretion body are found in sheep granulophilocyte for the first time, Johnstone in 1987
It is named as " exosome "；Various kinds of cell can secrete excretion body under normal and pathological state, be mainly derived from cell
Interior lysosome particle invaginates the more vesica bodies to be formed, and is discharged into extracellular matrix after the external film of more vesicas and cell membrane fusion；
The cell type of all cultures can secrete excretion body, and excretion body is naturally present in body fluid, deposit in person joint's liquid excretion body
In excretion body, in the prior art for the treatment of person joint's disease frequently with drug or operative treatment, therapeutic effect is general, to disease
The wound that human body generates is larger, and at present to the treatment method of arthropathy, most of to play relaxation effect, can not
Fundamentally radical curing of disease, for this purpose, it is proposed that a kind of preparation and its application method comprising person joint's liquid excretion body.
Summary of the invention
The purpose of the present invention is to provide a kind of preparations and its application method comprising person joint's liquid excretion body, to solve to carry on the back
The problem of being proposed in scape technology.
To achieve the above object, the present invention provides the following technical solutions：A kind of preparation comprising person joint's liquid excretion body, packet
Include following component (by mass percentage)：Person joint's liquid excretion body 20-30%, polyglycol solution 15-18%, the white egg of people's blood
White 7-12%, methionine 6.5-7.5%, propylene carbonate 5.3-6.7%, Catergen .1-3.8%, tranexamic acid 1.35-
2.28%, solvent 4.87-5.86%, phosphide acylsarcosine compound 5.66-7.32%, deionized water are surplus.
Preferably, including following component (by mass percentage)：Person joint's liquid excretion body 20%, polyglycol solution
15%, human serum albumin 7%, methionine 6.5%, propylene carbonate 5.3%, Catergen .1%, tranexamic acid 1.35%, molten
Matchmaker 4.87%, phosphide acylsarcosine compound 5.66%, deionized water are surplus.
Preferably, including following component (by mass percentage)：Person joint's liquid excretion body 23%, polyglycol solution
16%, human serum albumin 8%, methionine 6.8%, propylene carbonate 5.7%, Catergen .6%, tranexamic acid 1.68%, molten
Matchmaker 5.14%, phosphide acylsarcosine compound 5.88%, deionized water are surplus.
Preferably, including following component (by mass percentage)：Person joint's liquid excretion body 27%, polyglycol solution
17%, human serum albumin 10%, methionine 7.2%, propylene carbonate 6.2%, vitamin C 3.5%, tranexamic acid 2.12%,
Solvent 5.48%, phosphide acylsarcosine compound 6.92%, deionized water are surplus.
Preferably, including following component (by mass percentage)：Person joint's liquid excretion body 30%, polyglycol solution
18%, human serum albumin 12%, methionine 7.5%, propylene carbonate 6.7%, vitamin C 3.8%, tranexamic acid 2.28%,
Solvent 5.86%, phosphide acylsarcosine compound 7.32%, deionized water are surplus.
Preferably, the solvent is any one of Multiple electrolytes injection, glucose injection or physiological saline；Institute
Phosphide acylsarcosine compound is stated to be made of the dioctylis sulfosuccinas natricus of phosphide acylsarcosine 45% and 55%.
A kind of preparation method of the preparation comprising person joint's liquid excretion body, includes the following steps：
Step 1：It takes person joint's liquid to be placed in sterile centrifugation tube, utilizes the excretion body in supercentrifugation separation joint fluid；
Centrifugation step is as follows：300g, 4 DEG C, 15 minutes；3000g, 4 DEG C, 15 minutes；20000g, 4 DEG C, 70 minutes；0.22 micron of sieve
Son filtering；120000g, 120 minutes, obtains person joint's liquid excretion body by 4 DEG C；
Step 2：Prepare preparation：Human serum albumin, methionine, vitamin and tranexamic acid is taken to be placed in high speed agitator,
It is stirred 15-25 minutes with the speed of 600-800 turns/min；Then propylene carbonate, phosphide acylsarcosine are added into blender
Compound, solvent and deionized water, improving mixing speed is that 1000-1200 turns/min, and temperature is maintained at 25-35 DEG C when stirring,
Centrifugal filtration 10-20 minutes after stirring, filtered fluid and person joint's liquid excretion body is taken to be mixed to get preparation；
Step 3：Degerming：Preparation obtained in step 2 is taken to be sent into filtration sterilization in degerming grade hydrophilic filters；It is hydrophilic
Property filter aperture be 0.1~1 μm；
Step 4：Recovery freezes：Preparation after taking filtration sterilization is added water bath with thermostatic control and recovers, and preparation is added and saves pipe
In, pre-cooling 20-30 minutes is then carried out, liquid nitrogen container cryopreservation is eventually adding；
Step 5：Injection uses：It takes out preparation and saves pipe, then carried out disinfection using syringe, syringe sucks preparation
The preparation in pipe is saved, is injected at patient articular.
Compared with prior art, the beneficial effects of the invention are as follows：Formula design of the invention is more reasonable, is mentioned by separation
Person joint's liquid excretion body is obtained, by adding human serum albumin, vitamin C, methionine, ammonia first ring to person joint's liquid excretion body
Substance needed for a variety of joint fluids such as acid, propylene carbonate has good curative effect for treatment arthropathy, controls compared to drug or operation
It treats, the use of the excretion body preparation is more safe, reduces the wound caused by patient body；And the excretion body preparation exists
Can be with long-term preservation after being made, excretion body preparation is also very convenient when in use, and highly-safe, therapeutic effect is good, conducive to pushing away
Wide application.
Specific embodiment
Below in conjunction with the embodiment of the present invention, technical scheme in the embodiment of the invention is clearly and completely described,
Obviously, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Based in the present invention
Embodiment, every other embodiment obtained by those of ordinary skill in the art without making creative efforts, all
Belong to the scope of protection of the invention.
Embodiment one：
A kind of preparation comprising person joint's liquid excretion body, including following component (by mass percentage)：Outside person joint's liquid
Secrete body 20%, polyglycol solution 15%, human serum albumin 7%, methionine 6.5%, propylene carbonate 5.3%, vitamin
C2.1%, tranexamic acid 1.35%, solvent 4.87%, phosphide acylsarcosine compound 5.66%, deionized water are surplus.
Further, solvent is Multiple electrolytes injection；Phosphide acylsarcosine compound is by phosphide acylsarcosine 45%
It is formed with 55% dioctylis sulfosuccinas natricus.
A kind of preparation method of the preparation comprising person joint's liquid excretion body, includes the following steps：
Step 1：The separation and Extraction of person joint's liquid excretion body：Take person joint's liquid to be placed in sterile centrifugation tube, using hypervelocity from
Heart method separates the excretion body in joint fluid；Centrifugation step is as follows：300g, 4 DEG C, 15 minutes；3000g, 4 DEG C, 15 minutes；
20000g, 4 DEG C, 70 minutes；0.22 micron of screen filtration；120000g, 120 minutes, obtains person joint's liquid excretion body by 4 DEG C；
Step 2：Prepare preparation：Human serum albumin, methionine, vitamin and tranexamic acid is taken to be placed in high speed agitator,
It is stirred 15 minutes with the speed of 600 turns/min；Then into blender be added propylene carbonate, phosphide acylsarcosine compound,
Solvent and deionized water, raising mixing speed are 1000 turns/min, and temperature is maintained at 25 DEG C when stirring, is centrifuged after stirring
Filter 10 minutes takes filtered fluid and person joint's liquid excretion body to be mixed to get preparation；
Step 3：Degerming：Preparation obtained in step 2 is taken to be sent into filtration sterilization in degerming grade hydrophilic filters；It is hydrophilic
Property filter aperture be 0.1 μm；
Step 4：Recovery freezes：Preparation after taking filtration sterilization is added water bath with thermostatic control and recovers, and preparation is added and saves pipe
In, pre-cooling 20 minutes is then carried out, liquid nitrogen container cryopreservation is eventually adding；
Step 5：Injection uses：It takes out preparation and saves pipe, then carried out disinfection using syringe, syringe sucks preparation
The preparation in pipe is saved, is injected at patient articular.
Embodiment two：
A kind of preparation comprising person joint's liquid excretion body, including following component (by mass percentage)：Outside person joint's liquid
Secrete body 23%, polyglycol solution 16%, human serum albumin 8%, methionine 6.8%, propylene carbonate 5.7%, vitamin
C2.6%, tranexamic acid 1.68%, solvent 5.14%, phosphide acylsarcosine compound 5.88%, deionized water are surplus.
Further, solvent is glucose injection；Phosphide acylsarcosine compound is by 45% He of phosphide acylsarcosine
55% dioctylis sulfosuccinas natricus composition.
A kind of preparation method of the preparation comprising person joint's liquid excretion body, includes the following steps：
Step 1：The separation and Extraction of person joint's liquid excretion body：Take person joint's liquid to be placed in sterile centrifugation tube, using hypervelocity from
Heart method separates the excretion body in joint fluid；Centrifugation step is as follows：300g, 4 DEG C, 15 minutes；3000g, 4 DEG C, 15 minutes；
20000g, 4 DEG C, 70 minutes；0.22 micron of screen filtration；120000g, 120 minutes, obtains person joint's liquid excretion body by 4 DEG C；
Step 2：Prepare preparation：Human serum albumin, methionine, vitamin and tranexamic acid is taken to be placed in high speed agitator,
It is stirred 18 minutes with the speed of 650 turns/min；Then into blender be added propylene carbonate, phosphide acylsarcosine compound,
Solvent and deionized water, raising mixing speed are 1050 turns/min, and temperature is maintained at 28 DEG C when stirring, is centrifuged after stirring
Filter 13 minutes takes filtered fluid and person joint's liquid excretion body to be mixed to get preparation；
Step 3：Degerming：Preparation obtained in step 2 is taken to be sent into filtration sterilization in degerming grade hydrophilic filters；It is hydrophilic
Property filter aperture be 0.4 μm；
Step 4：Recovery freezes：Preparation after taking filtration sterilization is added water bath with thermostatic control and recovers, and preparation is added and saves pipe
In, pre-cooling 24 minutes is then carried out, liquid nitrogen container cryopreservation is eventually adding；
Step 5：Injection uses：It takes out preparation and saves pipe, then carried out disinfection using syringe, syringe sucks preparation
The preparation in pipe is saved, is injected at patient articular.
Embodiment three：
A kind of preparation comprising person joint's liquid excretion body, including following component (by mass percentage)：Outside person joint's liquid
Secrete body 27%, polyglycol solution 17%, human serum albumin 10%, methionine 7.2%, propylene carbonate 6.2%, vitamin
C3.5%, tranexamic acid 2.12%, solvent 5.48%, phosphide acylsarcosine compound 6.92%, deionized water are surplus.
Further, solvent is physiological saline；Phosphide acylsarcosine compound is by the sulphur of phosphide acylsarcosine 45% and 55%
Base sodium succinate dioctyl ester composition.
A kind of preparation method of the preparation comprising person joint's liquid excretion body, includes the following steps：
Step 1：The separation and Extraction of person joint's liquid excretion body：Take person joint's liquid to be placed in sterile centrifugation tube, using hypervelocity from
Heart method separates the excretion body in joint fluid；Centrifugation step is as follows：300g, 4 DEG C, 15 minutes；3000g, 4 DEG C, 15 minutes；
20000g, 4 DEG C, 70 minutes；0.22 micron of screen filtration；120000g, 120 minutes, obtains person joint's liquid excretion body by 4 DEG C；
Step 2：Prepare preparation：Human serum albumin, methionine, vitamin and tranexamic acid is taken to be placed in high speed agitator,
It is stirred 22 minutes with the speed of 750 turns/min；Then into blender be added propylene carbonate, phosphide acylsarcosine compound,
Solvent and deionized water, raising mixing speed are 1150 turns/min, and temperature is maintained at 33 DEG C when stirring, is centrifuged after stirring
Filter 17 minutes takes filtered fluid and person joint's liquid excretion body to be mixed to get preparation；
Step 3：Degerming：Preparation obtained in step 2 is taken to be sent into filtration sterilization in degerming grade hydrophilic filters；It is hydrophilic
Property filter aperture be 0.8 μm；
Step 4：Recovery freezes：Preparation after taking filtration sterilization is added water bath with thermostatic control and recovers, and preparation is added and saves pipe
In, pre-cooling 28 minutes is then carried out, liquid nitrogen container cryopreservation is eventually adding；
Step 5：Injection uses：It takes out preparation and saves pipe, then carried out disinfection using syringe, syringe sucks preparation
The preparation in pipe is saved, is injected at patient articular.
Example IV：
A kind of preparation comprising person joint's liquid excretion body, including following component (by mass percentage)：Outside person joint's liquid
Secrete body 30%, polyglycol solution 18%, human serum albumin 12%, methionine 7.5%, propylene carbonate 6.7%, vitamin
C3.8%, tranexamic acid 2.28%, solvent 5.86%, phosphide acylsarcosine compound 7.32%, deionized water are surplus.
Further, solvent is glucose injection；Phosphide acylsarcosine compound is by 45% He of phosphide acylsarcosine
55% dioctylis sulfosuccinas natricus composition.
A kind of preparation method of the preparation comprising person joint's liquid excretion body, includes the following steps：
Step 1：The separation and Extraction of person joint's liquid excretion body：Take person joint's liquid to be placed in sterile centrifugation tube, using hypervelocity from
Heart method separates the excretion body in joint fluid；Centrifugation step is as follows：300g, 4 DEG C, 15 minutes；3000g, 4 DEG C, 15 minutes；
20000g, 4 DEG C, 70 minutes；0.22 micron of screen filtration；120000g, 120 minutes, obtains person joint's liquid excretion body by 4 DEG C；
Step 2：Prepare preparation：Human serum albumin, methionine, vitamin and tranexamic acid is taken to be placed in high speed agitator,
It is stirred 25 minutes with the speed of 800 turns/min；Then into blender be added propylene carbonate, phosphide acylsarcosine compound,
Solvent and deionized water, raising mixing speed are 1200 turns/min, and temperature is maintained at 35 DEG C when stirring, is centrifuged after stirring
Filter 20 minutes takes filtered fluid and person joint's liquid excretion body to be mixed to get preparation；
Step 3：Degerming：Preparation obtained in step 2 is taken to be sent into filtration sterilization in degerming grade hydrophilic filters；It is hydrophilic
Property filter aperture be 1 μm；
Step 4：Recovery freezes：Preparation after taking filtration sterilization is added water bath with thermostatic control and recovers, and preparation is added and saves pipe
In, pre-cooling 30 minutes is then carried out, liquid nitrogen container cryopreservation is eventually adding；
Step 5：Injection uses：It takes out preparation and saves pipe, then carried out disinfection using syringe, syringe sucks preparation
The preparation in pipe is saved, is injected at patient articular.
The preparation of excretion body can be made in above four groups of embodiments, and formula design of the invention is more reasonable, passes through
Separation and Extraction obtains person joint's liquid excretion body, by person joint's liquid excretion body add human serum albumin, vitamin C, methionine,
Substance needed for a variety of joint fluids such as tranexamic acid, propylene carbonate has good curative effect for treatment arthropathy, compared to drug or
The use of operative treatment, the excretion body preparation is more safe, reduces the wound caused by patient body；And the excretion body
Preparation can be with long-term preservation after being made, and excretion body preparation is also very convenient when in use, and highly-safe, therapeutic effect is good,
Conducive to popularization and application.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (7)
1. a kind of preparation comprising person joint's liquid excretion body, which is characterized in that including following component (by mass percentage)：People
Joint fluid excretion body 20-30%, polyglycol solution 15-18%, human serum albumin 7-12%, methionine 6.5-7.5%, propylene
Carbonic ester 5.3-6.7%, Catergen .1-3.8%, tranexamic acid 1.35-2.28%, solvent 4.87-5.86%, phosphatidyl flesh
Propylhomoserin compound 5.66-7.32%, deionized water are surplus.
2. a kind of preparation comprising person joint's liquid excretion body according to claim 1, which is characterized in that including following component
(by mass percentage)：Person joint's liquid excretion body 20%, polyglycol solution 15%, human serum albumin 7%, methionine
6.5%, propylene carbonate 5.3%, Catergen .1%, tranexamic acid 1.35%, solvent 4.87%, phosphide acylsarcosine are compound
Object 5.66%, deionized water are surplus.
3. a kind of preparation comprising person joint's liquid excretion body according to claim 1, which is characterized in that including following component
(by mass percentage)：Person joint's liquid excretion body 23%, polyglycol solution 16%, human serum albumin 8%, methionine
6.8%, propylene carbonate 5.7%, Catergen .6%, tranexamic acid 1.68%, solvent 5.14%, phosphide acylsarcosine are compound
Object 5.88%, deionized water are surplus.
4. a kind of preparation comprising person joint's liquid excretion body according to claim 1, which is characterized in that including following component
(by mass percentage)：Person joint's liquid excretion body 27%, polyglycol solution 17%, human serum albumin 10%, methionine
7.2%, propylene carbonate 6.2%, vitamin C 3.5%, tranexamic acid 2.12%, solvent 5.48%, phosphide acylsarcosine are compound
Object 6.92%, deionized water are surplus.
5. a kind of preparation comprising person joint's liquid excretion body according to claim 1, which is characterized in that including following component
(by mass percentage)：Person joint's liquid excretion body 30%, polyglycol solution 18%, human serum albumin 12%, methionine
7.5%, propylene carbonate 6.7%, vitamin C 3.8%, tranexamic acid 2.28%, solvent 5.86%, phosphide acylsarcosine are compound
Object 7.32%, deionized water are surplus.
6. a kind of preparation comprising person joint's liquid excretion body according to claim 1, it is characterised in that：The solvent is multiple
Any one of square electrolyte injection, glucose injection or physiological saline；The phosphide acylsarcosine compound is by phosphide
The dioctylis sulfosuccinas natricus of acylsarcosine 45% and 55% composition.
7. a kind of preparation method of the preparation comprising person joint's liquid excretion body as described in claim 1-6, which is characterized in that packet
Include following steps：
Step 1：The separation and Extraction of person joint's liquid excretion body：It takes person joint's liquid to be placed in sterile centrifugation tube, utilizes supercentrifugation
Separate the excretion body in joint fluid；Centrifugation step is as follows：300g, 4 DEG C, 15 minutes；3000g, 4 DEG C, 15 minutes；20000g, 4
DEG C, 70 minutes；0.22 micron of screen filtration；120000g, 120 minutes, obtains person joint's liquid excretion body by 4 DEG C；
Step 2：Prepare preparation：Human serum albumin, methionine, vitamin and tranexamic acid is taken to be placed in high speed agitator, with
The speed of 600-800 turns/min stirs 15-25 minutes；Then it is multiple that propylene carbonate, phosphide acylsarcosine are added into blender
Object, solvent and deionized water are closed, improving mixing speed is that 1000-1200 turns/min, and temperature is maintained at 25-35 DEG C when stirring, is stirred
Centrifugal filtration 10-20 minutes after mixing, filtered fluid and person joint's liquid excretion body is taken to be mixed to get preparation；
Step 3：Degerming：Preparation obtained in step 2 is taken to be sent into filtration sterilization in degerming grade hydrophilic filters；Hydrophily mistake
The aperture of filter is 0.1~1 μm；
Step 4：Recovery freezes：Preparation after taking filtration sterilization is added water bath with thermostatic control and recovers, and preparation is added and saves in pipe,
Then pre-cooling 20-30 minutes is carried out, liquid nitrogen container cryopreservation is eventually adding；
Step 5：Injection uses：It takes out preparation and saves pipe, then carried out disinfection using syringe, syringe sucks preparation and saves
Preparation in pipe, is injected at patient articular.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110840915A (en) * | 2019-11-25 | 2020-02-28 | 深圳市第二人民医院 | Preparation method and application of targeted mesenchymal stem cell exosome |
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