CN1088117A - High-efficiency biogum - Google Patents
High-efficiency biogum Download PDFInfo
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- CN1088117A CN1088117A CN 92114810 CN92114810A CN1088117A CN 1088117 A CN1088117 A CN 1088117A CN 92114810 CN92114810 CN 92114810 CN 92114810 A CN92114810 A CN 92114810A CN 1088117 A CN1088117 A CN 1088117A
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- agent
- biogum
- efficiency
- bonding
- thrombin
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- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
High-efficiency biogum belongs to medical biotechnology binding agent class.Adopt the high-efficiency biogum of the I agent of Fibrinogen, calcium chloride, the XIII factor, aprotinin composition and the II agent composition that thrombin is formed.Use preceding with two doses of normal saline water preparations in calorstat melt into 2.5; During use the I agent of melt into, II agent water preparation are mixed, be coated with on the section of osteocomma, make that osteocomma is bonding to be resetted; The bonding strength height is arranged, and resetting property is good, and fast characteristics heal.
Description
The invention belongs to medical biotechnology binding agent class.
Treat complicated comminuted fracture both at home and abroad, particularly IA fracture is difficulty very.Less sclerite is threaded a needle with screw, bone garden pin etc. and easily is broken into littler osteocomma, can not fix; Bigger sclerite reset with, easily displacement is difficult for reaching the purpose of anatomical reduction in the process of dress inner fixer.After repairing Movable joint swelling is arranged more, pain, to such an extent as to form traumatic arthritis, therapeutic effect at a specified future date is undesirable.In recent years, extract the existing report of application of binding agent from human blood, but the source of extraction organization material is abundant, and propagation hepatitis is arranged, potential dangers such as AIDS are not so there is extensive use.At present, existing biological tissue adhesives is used for the clinical report of skin adherent, but bonding strength does not still reach bonding sclerotin requirement.
The objective of the invention is: develop a kind of dynamical biological grain rubber alloy, be used for the bonding sclerite of complexity comminuted fracture and intra-articular fracture, can make sclerite after resetting be bonded in the locational high-efficiency biogum of correct anatomical physiology securely, the used biological raw material of biogum extracts from cattle, porcine blood serum, the source is abundant, and avoids the propagation of potential disease.
Measure of the present invention is: high-efficiency biogum is by celloglobulin, calcium chloride,
The factor, the II agent that worker's agent that aprotinin is formed and thrombin are formed is formed.
The access method that gets of each composition of high-efficiency biogum is:
1. blood plasma must be got: with cattle or pig whole blood, with conventional biochemical method, add Chinese holly rubber acid sodium anticoagulant, centrifugal separation plasma etc. are standby;
2. Fibrinogen extracts: electrical methods such as usefulness, and by extracting Fibrinogen in the blood plasma
3. thrombin extracts: electrical methods such as usefulness, and standby by extracting thrombin in the blood plasma;
4.
The factor is extracted: electrical methods such as usefulness, and by extracting in the blood plasma
The factor is standby
5. aprotinin is extracted: use affinity chromatography, by extracting in the pulmonis Bovis seu Bubali tissue.
6. calcium chloride is separated into calcium ion with chemical method.
More than various spare unit all through G5, G6 funnel sucking filtration is removed processing such as immunogen, antibacterial and virus.
The proportioning of the above various components of high-efficiency biogum is:
Fibrinogen: 10-100mg; Thrombin: 10-50mg;
The factor: 0.1-5.0mg; Aprotinin: 0.1-5.0mg; Calcium chloride: 10-50mmoe
Packing:
1, with the Fibrinogen of said ratio amount,
The factor, aprotinin, calcium chloride is bottled and make dried frozen aquatic products, sterile packaged, the mark mirror is the agent of high-efficiency biogum I.
2, it is bottled thrombin 10-50 to be made dried frozen aquatic products, and the mark mirror is the agent of high-efficiency biogum II.
Before the use, the agent of high-efficiency biogum I, II agent are used the normal saline water preparation of 37 ℃ of calorstat melt into 2.5mg respectively.I agent, II agent water preparation with melt into during use mix, and are evenly coated on the section of osteocomma, and it is bonding to reset by anatomical position, and clamp fixedly 3-5 minute is sewed up soft tissue.
The test of bonding strength and bonding force:
At the simulation physiological temp, PH, ionic strength, thrombin amount, the GUSUIPIAN that exsomatizes be with the proportioning of different component, and the fracture fragments that is adhesively fixed is determined the anti-stress after bonding, and anti-shearing intensity of force is selected best proportioning.To high-efficiency biogum Effect on Performance process, obtain to have enough strong bonding strength and bonding time.
Intensity experiment: on the basis of animal bone and human body bone test of exsomatizing, divide two groups of tests with one-tenth Canis familiaris L. in age: one group is adopted screw to fix, and another group adopts high-performance bio glue bond, same position sclerite, observe 2,4,6,8 all healing states, the mensuration adhesive tension is 140-160kg/cm
2, gluing healing strength ratio is more satisfactory.
Tissue slice is observed: glue is distributed as the guiding principle shape, the osteocyte fracture line of normally growing into, 8 weeks organize fracture line unclear, osteocyte is grown into fully with normally the position bone slice is consistent.(zero difference)
Toxicological experiment: high-efficiency biogum has no side effect.
High-efficiency biogum of the present invention can promote union of fracture, and absorbs drainage naturally after the union of fracture, and effect is comparatively desirable, is a kind of high-performance bio material.
Claims (2)
1, a kind of high-efficiency biogum is characterized in that:
The II agent that I agent of being made up of Fibrinogen, calcium chloride, the X III factor, aprotinin and thrombin are formed is formed.
2, by the described biogum of claim 1, it is characterized in that: the weight ratio of each composition is:
Fibrinogen: 10-100mg
Thrombin: 10-50mg
Aprotinin: 0.1-5.0mg
Calcium chloride: 10-50mmoe
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 92114810 CN1088117A (en) | 1992-12-14 | 1992-12-14 | High-efficiency biogum |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 92114810 CN1088117A (en) | 1992-12-14 | 1992-12-14 | High-efficiency biogum |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1088117A true CN1088117A (en) | 1994-06-22 |
Family
ID=4947131
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 92114810 Pending CN1088117A (en) | 1992-12-14 | 1992-12-14 | High-efficiency biogum |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1088117A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101214391B (en) * | 2007-12-27 | 2010-05-19 | 广州倍绣生物技术有限公司 | High-efficiency biogum sealant and uses thereof |
| CN102791299A (en) * | 2010-01-28 | 2012-11-21 | 奥姆里克斯生物药品有限公司 | Method for improved fibrin sealing |
-
1992
- 1992-12-14 CN CN 92114810 patent/CN1088117A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101214391B (en) * | 2007-12-27 | 2010-05-19 | 广州倍绣生物技术有限公司 | High-efficiency biogum sealant and uses thereof |
| CN102791299A (en) * | 2010-01-28 | 2012-11-21 | 奥姆里克斯生物药品有限公司 | Method for improved fibrin sealing |
| CN102791299B (en) * | 2010-01-28 | 2014-10-22 | 奥姆里克斯生物药品有限公司 | Fibrin substrate having improved sealing property and preparation and application thereof |
| US8962033B2 (en) | 2010-01-28 | 2015-02-24 | Omrix Biopharmaceuticals Ltd. | Method for improved fibrin sealing |
| US9302026B2 (en) | 2010-01-28 | 2016-04-05 | Omrix Biopharmaceuticals Ltd. | Method for improved fibrin sealing |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C01 | Deemed withdrawal of patent application (patent law 1993) | ||
| WD01 | Invention patent application deemed withdrawn after publication |