CN108802008A - A kind of method and apparatus detecting tumour cell in blood using stimlated Raman spectrum - Google Patents
A kind of method and apparatus detecting tumour cell in blood using stimlated Raman spectrum Download PDFInfo
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- CN108802008A CN108802008A CN201811065008.0A CN201811065008A CN108802008A CN 108802008 A CN108802008 A CN 108802008A CN 201811065008 A CN201811065008 A CN 201811065008A CN 108802008 A CN108802008 A CN 108802008A
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- 210000004881 tumor cell Anatomy 0.000 title claims abstract description 49
- 210000004369 blood Anatomy 0.000 title claims abstract description 34
- 239000008280 blood Substances 0.000 title claims abstract description 34
- 238000001237 Raman spectrum Methods 0.000 title claims abstract description 26
- 238000000034 method Methods 0.000 title claims abstract description 20
- 238000001069 Raman spectroscopy Methods 0.000 claims abstract description 35
- 239000000126 substance Substances 0.000 claims abstract description 26
- 238000012360 testing method Methods 0.000 claims abstract description 18
- 239000004615 ingredient Substances 0.000 claims abstract description 13
- 239000000203 mixture Substances 0.000 claims abstract description 9
- 239000011521 glass Substances 0.000 claims description 36
- 230000003287 optical effect Effects 0.000 claims description 20
- 230000005540 biological transmission Effects 0.000 claims description 4
- 230000009471 action Effects 0.000 claims description 3
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- 238000007405 data analysis Methods 0.000 claims description 2
- 238000013480 data collection Methods 0.000 claims description 2
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- 238000001514 detection method Methods 0.000 abstract description 11
- 208000005443 Circulating Neoplastic Cells Diseases 0.000 abstract description 10
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/65—Raman scattering
Abstract
A method of detecting tumour cell in blood using stimlated Raman spectrum, the chemical composition stimlated Raman spectrum database for establishing related substances in each associated tumor cells including one, the process of the excited Raman profile data of each associated tumor cells characteristic chemical ingredient of one test sample, the marker being imaged as excited Raman using the intrinsic molecular vibration raman spectral signal of tumour cell characteristic chemical ingredient, real time imagery is projected by the excited Raman based on bessel beam, measure the chemical substance of test sample, obtain the information of tumour cell specified chemical ingredient in entire test sample, compared by excited Raman data in database, so that it is determined that whether any class tumour cell contained in test sample.Additionally provide the device using tumour cell in stimlated Raman spectrum detection blood.The present invention is circulating tumor cell in the excited Raman projection Real-time Imaging blood based on bessel beam, realizes and is detected to the high sensitivity of different tumour cells.
Description
Technical field
The invention belongs to field of bioinformatics, are related to the detection method of tumour cell, specifically it is a kind of using by
Swash the method and apparatus of tumour cell in Raman spectrum detection blood.
Background technology
Malignant tumour DISTANT METASTASES IN be clinically solid malignant treatment failure or recurrence the main reason for one of.And it follows
The presence of ring tumour cell is exactly the root of solid malignant DISTANT METASTASES IN.Circulating tumor cell is to be present in blood to carry
The antigenicity of former tumour and the tumour cell of molecular genetic properties.Circulating tumor cell derives from primary tumo(u)r or metastatic tumor, when
The matrix environment of suitable organ, tissue is encountered, will settle down herein, grow i.e. tumorigenic transfer.However enter cycle
Most of tumour cell can all lose activity, only remote organ is reached less than 0.01%, by migration, adherency, mutually interpolymerized
Collection forms small cancer embolus, and under the conditions of suitable microenvironment, new tumour is generated, so as to cause the generation of metastases.Cause
This circulating tumor cell quantity is very rare and is hidden in tens billion of blood cells, is directed to the detection of circulating tumor cell at present
Great challenge.
U.S. National Institutes（NIH）It is just pointed out in annual cancer research report in 2011, passes through Analysis of New Technology
Circulating tumor cell is epoch-making achievement in cancer research and clinical treatment history, can be effectively reduced the diagnosis and treatment of cancer at
This.Circulating tumor cell is detected as the curative effects such as the relapse and metastasis of early detection tumour, assessment operation, radiotherapy, chemistry, judges in advance
Afterwards, determine that tumor cells feature, the suitable individualized treatment of selection etc. provide important foundation, it will be to cancer early stage
Investigation and personalized medicine generate revolutionary impact.
In the past ten years, circulating tumor cell detection technique has attracted the very big concern of industry.It is different according to mechanism,
These methods can be classified as at present：Immunocytochemical technique, RT-polymerase chain reaction, fluorescence in situ hybridization technique and
Flow cytometry etc..
However, current method often rely on fluorescent marker for chemistry targeting, this may upset in life system
Biological function.In addition blood sampling volume is relatively large, and screening efficiency is low, and detection means is single and quality is low, each to circulating tumor cell
The identification and analysis sensitivity of class molecular composition is limited.
Invention content
The purpose of the present invention is to provide a kind of methods and dress detecting tumour cell in blood using stimlated Raman spectrum
It sets, this method and apparatus for detecting tumour cell in blood will solve to detect tumour cell in the prior art
Method sensitivity is not high, blood sample handles cumbersome, the other reagents labels of needs and technical problem complicated for operation.
The present invention provides a kind of methods detecting tumour cell in blood using stimlated Raman spectrum, including walk as follows
Suddenly：
1) be directed to one group known to tumor cells specimens, the tumor cells specimens are measured in the way of stimulated Raman scattering,
The stimlated Raman spectrum data for obtaining the chemical composition of related substances in tumor cells specimens, it is thin to obtain tumour by data analysis
The feature stimlated Raman spectrum of chemical composition described in born of the same parents' sample, establishes database, and the database includes tumour cell
The excited Raman profile data collection of chemical composition described in sample；
2) the excited Raman profile data of associated tumor cells characteristic chemical ingredient in sample to be tested is tested, in this step,
Test sample enters one end of glass capillary under the action of a constant pressure, and test sample travels forward, and from described
Glass capillary the other end side outflow, the optics closed windows of both ends end to end of glass capillary, by test sample
It is placed between laser and photodetector and receives the measurement of stimlated Raman spectrum；
3) by step 2）The middle excited Raman characteristic spectrum for measuring associated tumor cells characteristic chemical ingredient in the sample to be tested obtained
Data and step 1）Tumour cell characteristic chemical ingredient excited Raman data in the database of middle foundation compare, and determine test specimens
Whether contain tumour cell in product.
Further, in step 2）In, the Stokes light beam that Stokes lasing light emitter is sent out is by beam splitter in sky
Between the upper pump beam sent out with a pump laser source overlap, the light beam being collinearly overlapped is sent to a pair of of axicon mirror, use
In generating annular beam, then annular beam guiding speculum is reflected, then is focused in glass capillary by object lens
In test sample, focus on light beam is in that bessel beam is distributed in the test sample, and the Bezier of transmission is collected by collecting lens
Then light beam is directed to photodetector, obtain and measure stimlated Raman spectrum.
Wherein, filter is fixed on before photodetector to remove Stokes light beam ingredient.
The present invention also provides it is a kind of realize the above method utilize tumour cell in stimlated Raman spectrum detection blood
Device, the device include a glass capillary, and the both ends of the glass capillary are respectively set that there are one the first light
Learn window and second optical window；Further include a pump laser source, in the light path of the pump laser source successively
The second collimation lens, beam splitter, a pair are provided with to axicon mirror and speculum, in the speculum and second light
There are one object lens for setting between learning window；The laser optical path that the beam splitter is sent out with the pump laser source is in 45 degree
Angle, the speculum and the angle that the beam splitter is in 90 degree, the speculum are anti-by the light beam of pump laser source
Inject glass capillary；Further include a Stokes lasing light emitter, in the beam splitter and the Stokes lasing light emitter
Between setting there are one the first collimation lens；The laser refraction that the beam splitter sends out Stokes lasing light emitter swashs to pumping
In the light path of light source, to reflect into glass capillary；It is received there are one being set gradually in the side of first optical window
Collect lens, filter and photodetector, the collecting lens, filter and photodetector are arranged to swash in the pumping
The light beam of light source reflected after light path on.
Further, on the side wall of the glass capillary of the second optical window side setting there are one sample into
Mouthful；There are one sample exports for setting on the side wall of the glass capillary of the first optical window side.
Invention demonstrates a method the excited Raman projection imagings based on bessel beam, are used for unmarked volume chemical imaging,
It can be with the chemical substance in fast quantification three-D volumes, to quick and precisely detect the tumour cell in blood.
Further, the bessel beam, by Gaussian beam by twin shaft pyramid mirror generate annular beam after by object
Mirror, which focuses, to be generated；
Further, the glass capillary, length are less than 20 millimeters, and diameter is less than 500 microns, so as to entire glass capillary
Interior blood sample carries out the excited Raman projection imaging based on bessel beam；
The present invention operation principle be：By blood sample under the action of a constant pressure enter glass capillary, blood with
Certain speed travels forward, and glass tube is vertically arranged between light source and photodetector.Stokes laser and pump laser source
Light beam weight merga pass a pair of axicon mirror, generates annular beam and focuses on blood sample in glass capillary by object lens
On, focus on light beam has bessel beam feature distribution, can keep focusing over long distances along capillary, almost cover entire hair
Blood sample in tubule.It is the focusing length along input bessel beam by the Raman signal that photodetector detects
On entire blood sample stimulated Raman scattering intensity integral, be total chemical composition of 3D volumes in entire focal zone, inspection
Efficiency is surveyed to greatly improve.It is lived simultaneously with Raman using the difference on the frequency between pumping incident laser and Stokes incident laser
Property molecule vibration frequency matching when, excitation generate resonant check, formed stimulated Raman scattering, substantially increase unimolecule spy
The sensitivity of survey provides high Raman signal light intensity or brightness, has the characteristics that chemical specificity is strong and the signal acquisition time is short.
The present invention is using the intrinsic molecular vibration raman spectral signal of tumour cell characteristic chemical ingredient as being excited to draw
The marker of graceful imaging projects real time imagery by the excited Raman based on bessel beam, and fast quantification measures entire capillary
The chemical substance of test sample in pipe selectively obtains the letter of tumour cell specified chemical ingredient in entire test sample
Breath.
The present invention be the stimulated Raman scattering imaging technique based on bessel beam be it is a kind of it is novel, be not necessarily to fluorescent marker
Imaging method.It is a kind of nonlinear coherent Raman scattering process, it inherits the molecular specificity of spontaneous Raman spectrum, can
It is imaged different kind organism molecule with the differentiation of selectivity；The extremely faint disadvantage of spontaneous Raman signal is overcome simultaneously, is greatly enhanced
Signal can realize quick living imaging, in the unmarked imaging of the detection of tumour, DNA, small-molecule drug transmission etc.
Have wide application prospects.
The present invention is compared with prior art, is had the technical effect that positive and apparent.The present invention is directed to circulating tumor cell
Clinical detection application demand, invented be based on bessel beam excited Raman projection Real-time Imaging blood in follow
Ring tumour cell.And the multidisciplinary technology such as combination Raman spectroscopy, nonlinear optical technique and oncobiology is intersected and is melted
It closes, a kind of device detecting tumour cell in blood using stimlated Raman spectrum of exploitation, the circulating tumor in efficient detection blood
Cell establishes real-time diagnosis platform, realizes and is detected to the high sensitivity of different tumour cells.Also it is that diagnosing tumour clinic turns simultaneously
It moves and treatment new strategy provides technical support of new generation.
Description of the drawings
Fig. 1 is a kind of structural schematic diagram detecting the device of tumour cell in blood using stimlated Raman spectrum of the present invention.
Wherein, 1：Photodetector；2：Filter；3：Collecting lens；4：First optical window；5：Glass capillary；6：Second optics
Window；7：Stokes lasing light emitter；8：First collimation lens, 9：Pump laser source；10：Second collimation lens；11：Beam splitter；
12：A pair of of axicon mirror；13:Speculum；14：Object lens；15：Blood sample import；16：Blood sample exports.
Specific implementation mode
Embodiment 1
As shown in Figure 1, the present invention provides a kind of device detecting tumour cell in blood using stimlated Raman spectrum, including one
A glass capillary 5, the both ends of the glass capillary 5 are respectively set that there are one the first optical window 4 and second light
Learn window 6；
Further include a pump laser source 9, the second collimation lens is disposed in the light path of the pump laser source 9
10, beam splitter 11, a pair are to axicon mirror 12 and speculum 13, in the speculum 13 and second optical window 6
Between setting there are one object lens 14；It is in 45 between the laser optical path that the beam splitter 11 and the pump laser source 9 are sent out
The angle of degree, is in 90 degree of angle between the speculum 13 and the beam splitter 11, and the speculum 13 will pump
The light beam of lasing light emitter 9 is reflected into glass capillary 5；
Further include a Stokes lasing light emitter 7, is arranged between the beam splitter 11 and the Stokes lasing light emitter 7
There are one the first collimation lenses 8；The laser refraction that the beam splitter 11 sends out Stokes lasing light emitter 7 is to pump laser source
Light path on, to reflect into glass capillary 5；
It is set gradually there are one collecting lens 3, filter 2 and photodetector 1 in the side of first optical window 4,
The light after the light beam of the pump laser source 9 is reflected is arranged in collecting lens 3, filter 2 and the photodetector 1
On the road.
Specifically, on the side wall of the glass capillary 5 of 6 side of the second optical window setting there are one sample into
Mouthful；
There are one sample exports for setting on the side wall of the glass capillary 5 of 4 side of the first optical window.
Embodiment 2
Using device shown in the embodiment of the present invention 1, the present invention carries out stimlated Raman spectrum analysis to the fat drips in blood.Fat drips
The hydrophobic core being made of Lipid monolayer and neutral fats is constituted, and many albumen are distributed in surface.It is one " inertia "
Cell inclusion.We take capillary glass length of tube 2mm, inside diameter：100um.We take 5 milliliters of blood samples to be pumped into hair
Thin glass tube recycles, and Raman diffused light is penetrated and is adjusted to 2,850 cm -1CH2Symmetric vibration, to be excited to fat drips
Raman image.Stokes lasing light emitter 7 generates two synchronous laser beams with pump laser source 9 with the repetition rate of 80MHz.Its
In, Stokes laser 7 has the fixed wave length of 1,040nm.Pumping laser 9 has 680 to 1,300nm wavelengthtunable.So
By beam splitter 11, spatially light beam combines afterwards.The light beam of overlapping is converted into annular beam using two axicon mirrors 12,
Then annular beam is directed to the reflection of speculum 13, is focused in glass capillary 5 on blood sample by object lens 14.Focus light
Beam is distributed in blood sample in bessel beam in entire glass capillary 5.After blood sample, we use 60 times of water
Object lens 3 are soaked to collect the bessel beam of transmission.This high NA targets ensure high RST collection efficiency, to prevent image background
Carry out Cross-phase Modulation.Transmitting light beam is filtered by filter 2 first, to remove Stokes light beam ingredient, so
It is oriented to photodetector 1 afterwards, photodetector 1 uses bulk silicon photodiode（S3994-01, Hamamatsu）.It is total to draw
Graceful image acquisition time is 1.2 seconds.
It is respectively by the pumping laser and Stokes laser power of the bessel beam center lobe of 10 times of object lens generation
0.6 and 25mW.By to 2,850 cm-1Locate excited Raman luminous intensity measurement, can clearly obtain total lipid content
0.12mM, the result demonstrate excited Raman micromole's sensitivity.
The present embodiment further highlights raw in the projection real time imagery quantization volume of the excited Raman based on bessel beam
The advantage of the speed of the global information of object molecule.
Notwithstanding the preferred embodiment of the present invention, it should be apparent to those skilled in the art that
Without departing from the spirit or scope of the present invention, it can carry out various modifications and change in the present invention.Therefore, this hair
The bright modifications and variations for being intended to the present invention that covering comes within the scope of the appended claims and their.
Claims (4)
1. a kind of method detecting tumour cell in blood using stimlated Raman spectrum, it is characterised in that include the following steps：1)
For tumor cells specimens known to one group, the tumor cells specimens are measured in the way of stimulated Raman scattering, are swollen
The stimlated Raman spectrum data of the chemical composition of related substances, tumor cells specimens are obtained by data analysis in oncocyte sample
Described in chemical composition feature stimlated Raman spectrum, establish database, the database includes in tumor cells specimens
The excited Raman profile data collection of the chemical composition；
2) the excited Raman profile data of associated tumor cells characteristic chemical ingredient in sample to be tested is tested, in this step,
Test sample enters one end of glass capillary under the action of a constant pressure, and test sample travels forward, and from described
Glass capillary the other end side outflow, the optics closed windows of both ends end to end of glass capillary, by test sample
It is placed between laser and photodetector and receives the measurement of stimlated Raman spectrum；
3) by the excited Raman characteristic spectrum of associated tumor cells characteristic chemical ingredient in the sample to be tested for measuring acquisition in step 2)
Data determine test specimens compared with the tumour cell characteristic chemical ingredient excited Raman data in the database established in step 1)
Whether contain tumour cell in product.
2. a kind of method detecting tumour cell in blood using stimlated Raman spectrum as described in claim 1, feature exist
In：In step 2), the Stokes light beam that Stokes lasing light emitter is sent out spatially is pumped with one by beam splitter
The pump beam that lasing light emitter is sent out overlaps, and the light beam being collinearly overlapped is sent to a pair of of axicon mirror, for generating annular beam,
Then annular beam guiding speculum is reflected, then is focused in glass capillary in test sample by object lens, focused
Light beam is in that bessel beam is distributed in the test sample, and the bessel beam of transmission is collected by collecting lens, is then led
To photodetector, obtains and measure stimlated Raman spectrum.
3. realize a kind of device detecting tumour cell in blood using stimlated Raman spectrum of claim 1 the method,
It is characterized in that：Including a glass capillary, the both ends of the glass capillary are respectively set that there are one the first optical windows
With second optical window；Further include a pump laser source, is disposed in the light path of the pump laser source
Second collimation lens, beam splitter, a pair are to axicon mirror and speculum, in the speculum and second optical window
Between setting there are one object lens；The beam splitter and the angle that the laser optical path that the pump laser source is sent out is in 45 degree,
The speculum reflects the light beam of pump laser source into hair with the angle that the beam splitter is in 90 degree, the speculum
Thin glass tube；Further include a Stokes lasing light emitter, is set between the beam splitter and the Stokes lasing light emitter
First collimation lens there are one setting；The laser refraction that Stokes lasing light emitter is sent out is arrived pump laser source by the beam splitter
In light path, to reflect into glass capillary；The side of first optical window set gradually there are one collecting lens,
Filter and photodetector, the collecting lens, filter and photodetector are arranged in the pump laser source
Light beam reflected after light path on.
4. a kind of device detecting tumour cell in blood using stimlated Raman spectrum according to claim 3, feature
Be include：There are one sample inlets for setting on the side wall of the glass capillary of the second optical window side；Institute
There are one sample exports for setting on the side wall of the glass capillary for the first optical window side stated.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110470646A (en) * | 2019-08-23 | 2019-11-19 | 成都大象分形智能科技有限公司 | Tumor tissues identifying system based on artificial intelligence and Raman spectrum |
| CN113916855A (en) * | 2021-09-29 | 2022-01-11 | 深圳大学 | Microscopic imaging device |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012102833A2 (en) * | 2011-01-24 | 2012-08-02 | President & Fellows Of Harvard College | Systems and methods for stimulated raman scattering flow-cytometry |
| US20160260513A1 (en) * | 2015-03-04 | 2016-09-08 | U.S. Army Research Laboratory Attn: Rdrl-Loc-I | Optical trap using a focused hollow-beam for trapping and holding both absorbing and non-absorbing airborne particles |
| WO2017053592A1 (en) * | 2015-09-23 | 2017-03-30 | The Regents Of The University Of California | Deep learning in label-free cell classification and machine vision extraction of particles |
| US20180238738A1 (en) * | 2017-02-23 | 2018-08-23 | Robert Alfano | Resonant stimulated raman scattering microscope |
-
2018
- 2018-09-12 CN CN201811065008.0A patent/CN108802008A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012102833A2 (en) * | 2011-01-24 | 2012-08-02 | President & Fellows Of Harvard College | Systems and methods for stimulated raman scattering flow-cytometry |
| US20160260513A1 (en) * | 2015-03-04 | 2016-09-08 | U.S. Army Research Laboratory Attn: Rdrl-Loc-I | Optical trap using a focused hollow-beam for trapping and holding both absorbing and non-absorbing airborne particles |
| WO2017053592A1 (en) * | 2015-09-23 | 2017-03-30 | The Regents Of The University Of California | Deep learning in label-free cell classification and machine vision extraction of particles |
| US20180238738A1 (en) * | 2017-02-23 | 2018-08-23 | Robert Alfano | Resonant stimulated raman scattering microscope |
Non-Patent Citations (2)
| Title |
|---|
| BRADLEY B. COLLIER等: "Non-Linear Optical Flow Cytometry Using a Scanned, Bessel Beam Light-Sheet", 《SCIENTIFIC REPORTS》, no. 5, 29 May 2015 (2015-05-29), pages 1 - 8 * |
| JUN MAKIHARA等: "Optical chromatography Size determination by eluting particles", 《TALANTA》, vol. 48, no. 3, 31 March 1999 (1999-03-31), pages 551 - 557, XP008010871, DOI: 10.1016/S0039-9140(98)00272-0 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110470646A (en) * | 2019-08-23 | 2019-11-19 | 成都大象分形智能科技有限公司 | Tumor tissues identifying system based on artificial intelligence and Raman spectrum |
| CN113916855A (en) * | 2021-09-29 | 2022-01-11 | 深圳大学 | Microscopic imaging device |
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