CN108795793A - The method that microorganism is prepared by continuous two-step reaction using rubbish - Google Patents
The method that microorganism is prepared by continuous two-step reaction using rubbish Download PDFInfo
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Abstract
It the present invention relates to the use of the method that rubbish prepares microorganism by continuous two-step reaction.Specifically, a kind of microbial fermentation of present invention offer or microbe preparation method, the method includes：(1) to carrying out anaerobic fermentation or dark fermentation suitable for the rubbish of fermentation, zymotic fluid is obtained；(2) zymotic fluid obtained to step (1) is separated by solid-liquid separation；(3) aerobic fermentation is carried out as substrate using the zymotic fluid that step (2) obtains.Volatile fatty acid (VFA) is made full use of the technique in the microorganism especially production process of yeast and microalgae by the present invention, the VFA obtained for rubbish fermentation provides a kind of new application direction, also a kind of comprehensive solution is provided for the processing of rubbish, the more valuable product in addition to biogas, hydrogen etc. can be obtained.
Description
Technical field
The invention belongs to field of microbial fermentation, and in particular to prepare microorganism by continuous two-step reaction using rubbish
Method.
Background technology
Currently, world population and global economy rapid development, cause the yield of municipal refuse waste also to increase substantially.Suitably
Effectively management carried out to rubbish have become reduce ecological degeneration degree, and be gradually transitions one of sustainable development society
Necessary means.
Sanitary landfills, During High-Temperature Composting and burning are the current domestic and international widely used domestic waste mainly side of processing
Formula.Wherein, it burns due to the advantages that its volume reduction effect is preferable, processing is thorough, Processes and apparatus relative maturity, is increasingly becoming industry
The main way of developed country's garbage disposal.But the construction of incineration plant and producing cost are high, electric energy valence caused by equipment
Value is well below expected sales volume, and expensive operating cost, makes general city be difficult to carry out at rubbish using which in addition
Reason, while the various metals contained in rubbish make burning have very high toxicity, also can generate secondary hazards to environment.
According to the natural society's economic condition and garbage property of countries in the world, every country has different in garbage disposal
Technology path.The food waste class organic matter of easily biological-degradable and moisture are up to 50~65% in China's house refuse, such
If crude waste is directly entered incineration plant or landfill factory, the recycling efficiency of energy resource be difficult to it is constantly improve, percolate and
The pollution sources such as foul gas are also difficult to be effectively controlled, while also having higher carbon footprint.Although burning away the refuse hair
Electricity will become the mainstream technology of China's domestic rubbish disposal within a few years, but have research prediction to show that biologic treating technique must
The key link that will optimize as China's future life refuse disposal system, and play irreplaceable important function.
In fermentation arts, rubbish fermentation technology is concerned in recent years, the technology can be completed at the same time garbage disposal and
The production of high added value chemical products, wherein majority is concentrated on by carrying out anaerobic fermentation to rubbish or sludge the like waste, is obtained
To hydrogen, biogas, ethyl alcohol, lactic acid or organic fertilizer etc., but obtained by anaerobic fermentation or dark fermentation about using urban waste
Volatile fatty acid recycles the technology of its production yeast and microalgae to be rarely reported.
Invention content
The present invention proposes a kind of intermediate product that will ferment --- volatile fatty acid (VFA) makes full use of microorganism especially
Be yeast and microalgae production process in technique, the VFA obtained for rubbish fermentation provides a kind of new application direction, also for
The processing of rubbish provides a kind of comprehensive solution, can obtain the more valuable production in addition to biogas, hydrogen etc.
Product.
Therefore, first aspect present invention provides a kind of microbial fermentation processes, the method includes：
(1) to carrying out anaerobic fermentation or dark fermentation suitable for the rubbish of fermentation, zymotic fluid is obtained；
(2) zymotic fluid obtained to step (1) is separated by solid-liquid separation；With
(3) aerobic fermentation is carried out as substrate using the zymotic fluid that step (2) obtains.
In one or more embodiments, the rubbish derives from organic garbage of city.
In one or more embodiments, the organic garbage of city be solid waste, liquid debris and it
Mixture.
In one or more embodiments, the solid waste is selected from：Castoff, kitchen garbage or they
Combination.
In one or more embodiments, the rubbish that fermentation is suitable for described in step (1) is to pass through pretreated rubbish.
In one or more embodiments, the pretreated step includes：2mm sieve mistakes will be used after refuse breaking
Sieve, and sieving is prevented from being acidified at being placed at 5 DEG C of temperature below.
In one or more embodiments, the grain size by pretreated rubbish is less than 2mm, pH 6.00~
Between 6.50.
In one or more embodiments, the pH by pretreated rubbish is between 6.20~6.30, preferably
Between 6.25 ± 0.03.
In one or more embodiments, the anaerobic fermentation of step (1) or dark fermentation are carried out under the following conditions：
Inoculum concentration：8~20%, such as 8~10%, 10~15% or 8~15%；
Fermentation temperature：20~55 DEG C, such as 25~35 DEG C or 28~32 DEG C；With
pH：6.0~8.0, such as 6.8~7.2.
In one or more embodiments, step (1) is fermented using anaerobic sludge, waterpower and solid retention time
It it is 4~8 days, control organic loading rate (OLR) is between 22.4~38.5g VS/L/d.
In one or more embodiments, the fermentation of step (1) is batch fermentation, semicontinuous fermentation or continuously ferments.
In one or more embodiments, semicontinuous fermentation and continuously ferment, the mode of feed supplement makes in fermentation tank
Organic acid content control in the range of 0~5%, such as control 0.1~0.5% either 1.0~2.0% or 2.0
~3.0% either in the range of 3.0~4.0% or 4.0~5.0%.
In one or more embodiments, in step (1), second is produced using zymogenic bacteria, hydrogen-producing acetogens, consumption hydrogen
Sour bacterium or its arbitrary combination carry out anaerobic fermentation to the rubbish suitable for fermentation.
In one or more embodiments, in step (1), anaerobism hair is carried out using or mixtures thereof yeast, acetic acid bacteria
Ferment.
In one or more embodiments, the yeast is saccharomyces cerevisiae, and the acetic acid bacteria is brewing acetic acid bacteria.
In one or more embodiments, in step (1), anaerobism hair is carried out using the mixed bacterial contained by anaerobic sludge
Ferment.
In one or more embodiments, the mixed bacterial contained by the anaerobic sludge contains from clostridium
Belong to, the bacterium of Enterobacter and genus lactubacillus.
In one or more embodiments, in step (1), using selected from hydrogen-producing acetogens (such as
Desulfotomaculum sp.Iso-W2), fungal component (such as Sedimentibacter sp.JN18- of hydrogen-producing acetogens
A14-H), the bacterium (such as Clostridium hydroxybenzoicum) of degradable amino acid or they two or more
Combination carries out anaerobic fermentation.
In one or more embodiments, organic acid contained in the zymotic fluid that is obtained through anaerobic fermentation in step (1)
For volatile organic acids.
In one or more embodiments, the volatile organic acids is selected from formic acid, acetic acid, propionic acid, lactic acid and butyric acid
In one kind or arbitrary a variety of combination.
In one or more embodiments, in the organic substance of the zymotic fluid obtained through anaerobic fermentation in step (1),
80% or more is volatile fatty acid.
In one or more embodiments, the pH of the zymotic fluid obtained through anaerobic fermentation in step (1) is 4.0~8.0
In the range of, such as 5.5~6.5,6.5~7.0 or 6.5~7.9.
In one or more embodiments, the dark fermentation described in step (1) is carried out using microalgae.
In one or more embodiments, the microalgae is selected from：Scenedesmus quadricauda, scenedesmus obliquus, marine diatom, micro- quasi- ball
Algae, chlorella or their two or more of combinations.
In one or more embodiments, the pH through the obtained zymotic fluid that secretly ferments in step (1) is 4.0~9.0
In range, such as 4.5~6.5,5.5~7.5 or 7.5~8.5.
In one or more embodiments, it is first that the rubbish, which passes through organic acid contained in the zymotic fluid for secretly fermenting and obtaining,
One kind in acid, acetic acid, propionic acid, lactic acid and butyric acid etc. or arbitrary a variety of combination.
In one or more embodiments, the rubbish through in the obtained zymotic fluid organic substance of secretly fermenting, 80% with
Upper is volatile fatty acid.
In one or more embodiments, the separation of solid and liquid of step (2) is carried out using filter membrane.
In one or more embodiments, using the separation of solid and liquid described in hollow-fibre membrane implementation steps (2).
In one or more embodiments, the aperture of the hollow-fibre membrane is between 0.15~0.30 μm, surface area
In 250~350cm2Between.
In one or more embodiments, in the zymotic fluid that step (2) obtains after being separated by solid-liquid separation, carbonaceous material is volatilization
Property organic acid and alcohols material.
In one or more embodiments, in the zymotic fluid that step (2) obtains after being separated by solid-liquid separation, carbonaceous material is first
Acid, acetic acid, propionic acid, isobutyric acid, valeric acid, isovaleric acid, methanol, one kind of ethyl alcohol or arbitrary a variety of combination.
In one or more embodiments, in the fermentation of step (3), the content of organic acid is 1~5% in zymotic fluid,
It is preferred that 2~4%.
In one or more embodiments, using selected from algae, yeast, Purple Nonsulfer Bacteria, mould or they
The microorganisms of two or more of combinations carry out the aerobic fermentation described in step (3).
In one or more embodiments, the yeast is selected from：Shallow white Cryptococcus (Cryptococcus
Albidus), curved Cryptococcus (Cryptococcus albidun), Lipomyces starkeyi (Lipomyces starkeyi),
Trichosporon pullulans (Trichospiron pullulans), oil-producing saccharomyces oleaginosus (Lipomyces lipofer), the red ferment of gluing
Female (Rhodotorula giutinis), the red winter spore of class yeast (Rhodosporidium toruloides), sub- sieve solution fat yeast
(Yarrowia lipolytica), candida utili (Candida utilis), candida tropicalis (Candida
Tropicalis), garden false yeast (Torula utilis), torulopsis (Torulopsis utilis), saccharomyces cerevisiae
(Saccharomyces cerevisiae), sub- sieve solution fat yeast (Yarrowia lipolytica) or their two kinds or more
The combination of kind.
In one or more embodiments, the Purple Nonsulfer Bacteria is selected from：Rhodopseudomonas palustris
(Rhodopseudomonas palustris), class rhodopseudomonas spheroid (Rhodobacter sphaeroides), the red vacation of acidophilus
Monad (Rhodopseudomonas acidophilus), capsula Rhodopseudomonas (Rhodopseudomonas
Capsulata), red spirillum (Rhodospirillum) or their two or more of combinations.
In one or more embodiments, the mould is selected from：Native mould (Asoergullus terreus), purple paralysis
Ergot (Clavicepspurpurea), sorghum pleat embrace smut (Tolyposporium), Mortierella alpina
(Mortierella alpina), Mortierella isabellina (Mortierrella isabellina) or theirs is two or more of
Combination.
In one or more embodiments, using selected from Scenedesmus quadricauda, scenedesmus obliquus, marine diatom, micro- quasi- ball algae, small
The microalgae of ball algae or their combination of two or more carries out the aerobic fermentation described in step (3).
In one or more embodiments, the aerobic fermentation is implemented as follows：
Inoculum concentration is usually 1~30%, such as 5~25% either 5~20% or 5~15%；
The content of initial organic acid in fermentation tank is controlled in the range of 1~5%, preferably 2~4%；
During the fermentation, the organic acid content in fermentation tank can be controlled 0.1~1.0%, such as 0.1~0.5%
In the range of；With
Fermentation temperature is usually in the range of 25~50 DEG C, for example, 28~45 DEG C, 30~45 DEG C or 40~50 DEG C.
In one or more embodiments, aerobic fermentation utilize yeast, microalgae, Purple Nonsulfer Bacteria, mould, or
Their two or more of combinations are implemented, and pH is controlled in the range of 7.0 ± 0.3.
In one or more embodiments, in aerobic fermentation, ventilatory capacity is controlled in 0.5~2.0vvm.
In one or more embodiments, aerobic fermentation is implemented using algae, range of the pH controls 8.0~10.0
It is interior.
Description of the drawings
Fig. 1：VFA yield changes with time during anaerobic fermentation of kitchen waste.
Fig. 2：VFA generating rates change with time during anaerobic fermentation of kitchen waste.
Fig. 3：Yeast dry weight, VFA contents change over time curve.
Fig. 4：VFA yield changes with time during anaerobic fermentation of kitchen waste.
Fig. 5：VFA generating rates change with time during anaerobic fermentation of kitchen waste.
Fig. 6：Microalgae and VFA content versus time curves.
Fig. 7：Yeast continuously ferments substantially flow.
Fig. 8：Microalgae continuously cultivates substantially flow.
Fig. 9：Garbage disposal substantially flow.
Specific implementation mode
It should be understood that within the scope of the present invention, above-mentioned each technical characteristic of the invention and have in below (eg embodiment)
It can be combined with each other between each technical characteristic of body description, to constitute preferred technical solution.
Provided herein is microbial fermentation processes, which continuously ferments including two steps, is joined jointly by a variety of floras
With fermentation.The microbial fermentation processes of the present invention can be used for the albumen or grease of microorganism preparation method and microorganism
In preparation method.
Herein, microorganism includes bacterium, fungi and algae.Bacterium can be this field conventionally used for microbial fermentation
Various bacteriums, including but not limited to Purple Nonsulfer Bacteria.
In certain embodiments, it can be selected from suitable for the Purple Nonsulfer Bacteria of this paper：Rhodopseudomonas palustris
(Rhodopseudomonas palustris), class rhodopseudomonas spheroid (Rhodobacter sphaeroides), the red vacation of acidophilus
Monad (Rhodopseudomonas acidophilus), capsula Rhodopseudomonas (Rhodopseudomonas
) and red spirillum (Rhodospirillum) capsulata.Any one in these Purple Nonsulfer Bacterias can be used, or
Use their two or more of combinations.
Fungi includes yeast and mould.Yeast suitable for this paper can be selected from：Shallow white Cryptococcus (Cryptococcus
Albidus), curved Cryptococcus (Cryptococcus albidun), Lipomyces starkeyi (Lipomyces starkeyi),
Trichosporon pullulans (Trichospiron pullulans), oil-producing saccharomyces oleaginosus (Lipomyces lipofer), the red ferment of gluing
Female (Rhodotorula giutinis), the red winter spore of class yeast (Rhodosporidium toruloides), sub- sieve solution fat yeast
(Yarrowia lipolytica), candida utili (Candida utilis), candida tropicalis (Candida
Tropicalis), garden false yeast (Torula utilis), torulopsis (Torulopsis utilis), saccharomyces cerevisiae
(Saccharomyces cerevisiae) and sub- sieve solution fat yeast (Yarrowia lipolytica).These yeast can be used
In any one, or use their two or more of combinations.
Mould suitable for methods described herein can be selected from：Native mould (Asoergullus terreus), purple paralysis ergot
(Clavicepspurpurea), sorghum pleat embraces smut (Tolyposporium), Mortierella alpina (Mortierella
) and Mortierella isabellina (Mortierrella isabellina) alpina.Any one in these moulds can be used, or make
With their two or more of combinations.
Algae can be selected from Scenedesmus quadricauda, scenedesmus obliquus, marine diatom, micro- quasi- ball algae and chlorella.These microalgaes can be used
In any one, or use their two or more of combinations.In certain embodiments, chlorella is seawater bead
Algae.
The microbial fermentation processes of this paper include two-step fermentation.First step fermentation is to utilize zymogenic bacteria, production hydrogen production second
The anaerobic fermentation that sour bacterium, consumption hydrogen acetogen or its arbitrary combination carry out, or the dark fermentation using microalgae progress.In certain implementations
In scheme, the symbiosis selected from hydrogen-producing acetogens (such as Desulfotomaculum sp.Iso-W2), hydrogen-producing acetogens is utilized
Bacterium (such as Sedimentibacter sp.JN18-A14-H), degradable amino acid bacterium (such as Clostridium
Hydroxybenzoicum) or their combination of two or more carries out anaerobic fermentation.In certain embodiments, using detesting
Mixed bacterial contained by oxygen sludge carries out anaerobic fermentation.In general, anaerobic sludge can be the kitchen garbage from sludge treatment plant
The anaerobic sludge that anaerobic fermentation system generates, in contained mixed bacterial containing from Clostridium bacterium, come from
The bacterium of Enterobacter, from the bacterium of genus lactubacillus or its arbitrary combine.In certain embodiments, yeast, acetic acid are utilized
Or mixtures thereof bacterium carries out anaerobic fermentation.Herein, yeast can be each primary yeast commonly used in the art, such as ferment of making wine
It is female；Acetic acid bacteria can for example make acetic acid bacteria.
When secretly being fermented using microalgae, in general, pretreated rubbish is mixed with microalgae, seed sludge.With wet basis
Meter, the total solids content of the seed sludge can in the range of 5~8%, the content of volatile solid can 1.5~3% model
It encloses, N content can be in the range of 0.2~0.6%, and C/N ratios can be in the range of 3~5, and organic component content can be 40~60%
In the range of, salinity can be that 10~14, pH is 7.0 ± 0.3.The addition of seed sludge, which can be, accounts for the 5 of total mixture quality
~15%, such as 8~12%.The seed sludge can be obtained first by previously described fresh anaerobic sludge domestication.Acclimation method can
Including：Processed rubbish and microalgae are added in fresh anaerobic sludge, after stirring evenly, 5~7 are tamed at 35 ± 1 DEG C
It, obtains the seed sludge that pH is 6.4 ± 0.2.
The fermentation substrate of first step fermentation of the present invention is rubbish.Rubbish can be organic waste, such as from city or agriculture
The organic waste in village.Organic garbage of city suitable for this paper can be solid waste, liquid debris and theirs is mixed
Close object.Solid waste is suitable for the invention to can be selected from：Castoff, kitchen garbage or combination thereof.
In general, after obtaining rubbish, preliminary classification and sorting are first carried out, rejects not available substance, such as kitchen garbage
In bone and polybag etc., then pre-processed.Conventional method can be used to be pre-processed, to obtain grain size in 2mm
Below, pretreating wastes of the pH between 6.00~6.50.Preferably, the pH of the pretreated rubbish is 6.20~6.30
Between, more preferably between 6.25 ± 0.03.Rubbish is crushed for example, crusher can be used, then crosses 2mm sieves, from
And grain size is obtained in 2mm grain sizes below.In general, the rubbish through break process is placed under low temperature (such as 5 DEG C or less), prevent
Acidification.
Pretreated rubbish is used as fermentation substrate, carries out anaerobic fermentation or dark fermentation.In general, in terms of rubbish quality,
Anaerobic fermentation or the inoculum concentration secretly fermented are 8~20%, such as 8~10%, 10~15% or 8~15%.Fermentation temperature is usual
In the range of 20~55 DEG C, such as 25~38 DEG C or 28~32 DEG C.The pH value of zymotic fluid is normally controlled in 6.0~8.0 model
In enclosing, such as 6.0~7.0 or 6.8~7.2.In certain embodiments, in anaerobic fermentation, oxidation-reduction potential (ORP value) is controlled
In the range of -250~150mV, to control micro- oxygen environment in fermentation tank.
Anaerobic fermentation or dark fermentation are batch fermentation, semicontinuous fermentation or continuously ferment.In semicontinuous fermentation, feed profile
Can be that continuous flow adds, noncontinuum flow adds or multicycle stream adds.The ingredient of feed supplement can be that one-component stream adds or multigroup shunting
Add.For example, the ingredient of feed supplement can only be Determination of Organic Acids, or can be prepared according to the Expenditure Levels of each ingredient in zymotic fluid
Feed supplement liquid containing the Multiple components including organic acid.In certain embodiments, the mode of feed supplement makes in fermentation tank
Organic acid content control in the range of 0~0.5%, such as control 0.05~0.5% either 0.1~0.4% or
0.1~0.3% either in the range of 0.2~0.5% or 0.2~0.4%.In general, the initial concentration of organic acid be 2~
4%, start stream plus organic acid or containing the zymotic fluid of organic acid when the concentration of organic acid in zymotic fluid is less than 0.5%, and control
The final concentration of organic acid is between previously described 0~0.5% in zymotic fluid.In certain embodiments, when having in zymotic fluid
The concentration of machine acid be less than 0.45%, such as less than 0.4%, less than 0.3%, less than 0.2% or less than 0.1% when start stream added with
Machine acid or organic acid fermentation liquid.In certain embodiments, flow plus organic acid or organic acid fermentation liquid be it is sterile, preferably
It is sterilized by way of micro-pore-film filtration.
If in general, fermentating liquid volume be more than fermenter volume 80%, can stream plus while blowing, to control zymotic fluid
Volume is within the 80% of fermenter volume.
When carrying out anaerobic fermentation or dark fermentation using batch fermentation or semicontinuous fermentation, fermentation period is usually 3~4 days.
Anaerobic fermentation or dark fermentation gained zymotic fluid can be used for aerobic fermentation.Microorganism used in aerobic fermentation can be
Wish the aerobic fermentation microorganism of mass production, including previously described various bacteriums, fungi and algae, such as yeast, purple are non-
Sulphur photosynthetic bacteria, mould or their two or more of combinations.
In general, anaerobic fermentation or dark fermentation gained zymotic fluid are separated by solid-liquid separation, filtration sterilization.Conventional filtering hand can be used
Section is filtered.For example, aperture can be used between 0.15~0.30 μm, surface area is in 250~350cm2Between hollow fibre
Dimension film is filtered.This kind of hollow-fibre membrane can be commercially available product, such as the production of Spectrum Laboratories companies
Product, membranous type MiniKros Sample Plus Filter Module.
After separation of solid and liquid, filtrate can be diluted to organic acid content be 1~5%, preferably 2~4% in the range of.It is logical
Often, the pH for being used for the zymotic fluid of aerobic fermentation is controlled in the range of 6.8 ± 0.5.
When aerobic fermentation, the zymotic fluid of above-mentioned processing is added in fermentation tank, initial organic acid contains in control fermentation tank
Amount is in the range of 1~5%, preferably 2~4%.During the fermentation, the organic acid content in fermentation tank can be controlled 0.1
In the range of~1.0%, such as 0.1~0.5%.Fermentation temperature usually in the range of 25~50 DEG C, for example, 28~45 DEG C or
30~45 DEG C.In certain embodiments, the temperature of aerobic fermentation of the present invention is in the range of 40~50 DEG C.PH is normally controlled in
In the range of 7.0 ± 0.3；Ventilatory capacity is normally controlled in 0.5~2.0vvm.Speed of agitator can be controlled according to real attenuation condition
System, for example, usually caning be controlled in the range of 150~500rpm.Inoculum concentration is usually 1~30%, such as 5~25% or 5
~20% or 5~15% etc..
When carrying out microdisk electrode, suitable growth conditions can be selected according to the optimum growing condition of different microalgae.For example,
Under normal conditions, for the temperature control of microdisk electrode in the range of 25~40 DEG C, the pH of culture solution can be controlled in 8.0~10.0
In the range of.
Aerobic fermentation (including microdisk electrode) can also be batch fermentation, semicontinuous fermentation or continuously ferment.Semicontinuous hair
In ferment, feed profile can be that continuous flow adds, noncontinuum flow adds or multicycle stream adds.The ingredient of feed supplement can be one-component stream
Add or multigroup shunting adds.For example, the ingredient of feed supplement can only be Determination of Organic Acids, or can be disappeared according to each ingredient in zymotic fluid
It consumes situation and prepares the feed supplement liquid containing the Multiple components including organic acid.In certain embodiments, the mode of feed supplement
So that the organic acid content in fermentation tank controls in the range of 0~0.5%, such as control 0.05~0.5% or 0.1
~0.4% either 0.1~0.3% either in the range of 0.2~0.5% or 0.2~0.4%.In general, organic acid is first
Begin a concentration of 2~4%, starts stream plus organic acid when the concentration of organic acid in zymotic fluid is less than 0.5% or containing the hair of organic acid
Zymotic fluid, and the final concentration of organic acid in zymotic fluid is controlled between previously described 0~0.5%.In certain embodiments, when
The concentration of organic acid is less than 0.45% in zymotic fluid, such as less than 0.4%, less than 0.3%, less than 0.2% or less than 0.1% when
Start stream plus organic acid or organic acid fermentation liquid.In certain embodiments, it is nothing to flow the organic acid added or organic acid fermentation liquid
Bacterium, it sterilizes preferably through the mode of micro-pore-film filtration.
If in general, fermentating liquid volume be more than fermenter volume 80%, can stream plus while blowing, to control zymotic fluid
Volume is within the 80% of fermenter volume.
When carrying out aerobic fermentation using batch fermentation or semicontinuous fermentation, fermentation period is usually 3~4 days.
After fermentation, conventional mode can be used to detach acquisition fermentative microorganism and extract interested in microorganism
Substance, such as grease or protein.
Fig. 7 shows the substantially flow that a yeast of the invention continuously ferments.Wherein, the rubbish through collection is preprocessed
Afterwards, anaerobic fermentation is first carried out, then filters anaerobic fermented liquid, then the zymotic fluid of filtering is used for the aerobic fermentation of yeast, from
And yeast is prepared.
Fig. 8 shows the substantially flow that a microalgae of the invention is continuously cultivated.Wherein, the rubbish through collection is preprocessed
Afterwards, it is first secretly fermented, then by dark filtering fermentation liquor, then the aerobic fermentation by the zymotic fluid of filtering for microalgae, to make
It is standby to obtain microalgae.
The garbage disposal flow of Fig. 9 display present invention.Through the rubbish of collection after preliminary classification and sorting, it is sent into pretreatment
The pretreated rubbish with grain size described herein and pH is prepared in device, and then microbe inoculation carries out anaerobic fermentation
Or the filtrate of acquisition is carried out the aerobic fermentation of yeast (such as oleaginous yeast) by dark fermentation, metabolin after filter device detaches,
Or the culture of microalgae, to which yeast and microalgae be prepared respectively.
The present invention has the following advantages：
First, no matter VFA yield is higher or relatively low, compared to it is existing it is direct purify obtain the technique of product VFA at
This, the value of VFA product itselfs is far below the value that purifying technique consumes.In the case, set forth herein by anaerobic fermentation with
The method that aerobic fermentation is combined substantially increases its economic benefit.
Secondly, in last raffinate of fermenting, since VFA contents are extremely low (being absorbed and utilized by strain), hardly
Carry out sewage disposal with to waste liquid, can direct emission, reduce operating cost from another point of view.
In brief, comprehensive solution proposed in this paper can convert garbage into have the product of economy, i.e. ferment
Mother/microalgae reduces cost for purification, substantially increases economic benefit.
The present invention will be hereafter illustrated in a manner of specific embodiment.It should be understood that these embodiments are only illustrative, and
It does not limit the scope of the invention.Used method, reagent, instrument etc. in embodiment, unless otherwise stated, being this field
Conventional method, reagent and instrument.In addition, the preferred scope or preferred technological means of each technological means described herein, phase
It can arbitrarily be combined between mutually.
Embodiment 1：Yeast is obtained using anaerobic fermentation, 30 DEG C of aerobic two-step fermentations when 25 DEG C of kitchen waste
Anaerobic fermentation is carried out to kitchen garbage in accordance with the following methods：Rubbish from cooking is tentatively sorted, wherein bone is rejected
After the not available substance such as head, hard object, chopsticks and polybag, after centrifugation, dehydration, crushed using food crusher,
2mm sieves are crossed, the rubbish from cooking that can be used for fermenting after being handled, wherein total VFA contents are 0.19 ± 0.05g/L, pH value is 6.0
Between~6.5.
The kitchen garbage of above-mentioned acquisition is poured into fermentation tank, high activity dried yeast is inoculated with respectively by the 10% of rubbish quality
(main component is saccharomyces cerevisiae,18824TM) and acetic acid bacteria (main component is brewing acetic acid bacteria,
700178TM), controlling reaction temperature is 25 DEG C, pH 6.0, and ORP value (i.e. oxidation-reduction potential) is limited in -250~150mV, control
Micro- oxygen environment is made, starts to ferment.ORP value is measured using ORP meters (PHS-3C, Shanghai thunder magnetic).This fermentation process is using continuous hair
Ferment mode.
The measurement of VFA：Fermentation broth sample is centrifuged into 20min with the rotating speed of 5000r/min, takes supernatant, it is micro- through 0.45 μm
After the filter membrane of hole, into efficient liquid phase chromatographic analysis, wherein liquid chromatogram selects Shimadzu LC-10AS (UV-VIS SPD-10A types
Detector), mobile phase is 4mm sulfuric acid, flow velocity 0.6ml/min.
Testing result shows that machine object contained in garbage fermentation liquid is mostly VFA, and has a small amount of methane, carbon dioxide and hydrogen
Gas generates.Wherein, the VFA contents that the kitchen garbage after inoculation yeast bacterium and acetic acid bacteria ferments are up to 38.71g/L, second
Acid contains 81.8% (mass fraction), and specific VFA yield, which changes over time, sees Fig. 1.Ferment initial 2h when, VFA throughput rates reach
Peak 1.55g/ (Lh), specific generating rate are shown in Fig. 2.
By gained zymotic fluid through membrane filtration, the zymotic fluid that carbonaceous material is only VFA and a small amount of alcohols material is obtained, with distillation
Water mixed diluting enters to ferment in next step.
Aerobic fermentation is carried out to gained zymotic fluid in accordance with the following methods：By sub- sieve solution fat yeast liquid (Yarrowia
Lipolytica it) is transferred in 100L fermentation tanks and cultivates according to 10% inoculum concentration, 30 DEG C, speed of agitator 300rpm of temperature, ventilation
1.5vvm, pH are controlled 7.0, wherein the culture medium group of 100L fermentation tanks becomes：With 0.22 μm of pore size ceramic membrane filtration sterilization
Zymotic fluid (wherein acetic acid content is about 2.5%) 50kg, pH value 6.8, while flowing and adding the above-mentioned zymotic fluid after filtration sterilization, root
According to data in Fig. 3, the acetic acid content in 100L fermentation tanks is controlled about 0.1~0.4%, when liquid level is more than 80L, flow putting of adding
Material should control liquid level in 80L.This fermentation process is using mode of continuously fermenting.
The measurement of dry cell weight：After fermentation broth sample is weighed, 2min is centrifuged with the rotating speed of 4000rpm, gained precipitation adds
Washing is primary, then centrifuges 2min with the rotating speed of 4000rpm, weighs after the drying overnight of direct 80 DEG C of gained precipitation, it is dry to measure cell
Weight is 5.02g/kg zymotic fluids, i.e. every liter of kitchen garbage can produce to obtain about 15.484g yeast.
The measurement of cell grease：After fermentation broth sample is weighed, 2min is centrifuged with the rotating speed of 4000rpm, gained precipitation adds
Washing is primary, then centrifuges 2min with the rotating speed of 4000rpm, and the ether-petroleum ether (body of 4 times of fermentating liquid volumes is added in gained precipitation
Product ratio 1：2), room temperature extracts 2h, and pure water is then added and shakes split-phase, pipettes after organic phase is blown to constant weight with nitrogen and weighs, measure
Cell grease is 1.86g/kg zymotic fluids, accounts for the 37.05% of dry cell weight.
The measurement of cell protein：After fermentation broth sample is weighed, measured in cell using kelvin (Kjeldahl) nitriding
The content of protein, it is 1.52g/kg zymotic fluids to measure cell protein, accounts for the 30.2% of dry cell weight.
The measurement of remaining ferment liquid hold-up：Remaining ferment liquid component content such as table 1.
Table 1：Fermentation broth contents content after kitchen waste anaerobism, aerobic fermentation
| Ingredient | Content (g/L) |
| VFA | 0.0623 |
| Methanol | 0.0079 |
Embodiment 2：Aerobic two-step fermentation obtains yeast when using anaerobic fermentation when 25 DEG C of kitchen waste, 45 DEG C
Anaerobic fermentation is carried out to kitchen garbage in accordance with the following methods：Rubbish from cooking is tentatively sorted, wherein bone is rejected
After the not available substance such as head, hard object, chopsticks and polybag, after centrifugation, dehydration, crushed using food crusher,
Sieving, the rubbish from cooking that can be used for fermenting after handle, wherein total VFA contents are 0.19 ± 0.05g/L, pH value 6.0~
Between 6.5.
Above-mentioned processed kitchen garbage is poured into fermentation tank, high activity dried yeast is inoculated with respectively by the 10% of rubbish quality
(main component is saccharomyces cerevisiae,18824TM) and acetic acid bacteria (main component is brewing acetic acid bacteria,
700178TM), controlling reaction temperature is 25 DEG C, pH 6.0, and ORP value (i.e. oxidation-reduction potential) is limited in -250~150mV, control
Micro- oxygen environment is made, starts to ferment.ORP value is measured using ORP meters (PHS-3C, Shanghai thunder magnetic).This fermentation process is using semicontinuous
Fermentation method, period are 4 days.
The measurement of VFA：Fermentation broth sample is centrifuged into 20min with the rotating speed of 5000r/min, takes supernatant, it is micro- through 0.45 μm
After the filter membrane of hole, into efficient liquid phase chromatographic analysis, wherein liquid chromatogram selects Shimadzu LC-10AS (UV-VIS SPD-10A types
Detector), mobile phase is 4mm sulfuric acid, flow velocity 0.6ml/min.
Testing result shows that machine object contained in garbage fermentation liquid is mostly VFA, and has a small amount of methane, carbon dioxide and hydrogen
Gas generates.Wherein, the VFA contents that the kitchen garbage after inoculation yeast bacterium and acetic acid bacteria ferments are up to 38.71g/L, second
Acid contains 81.8% (mass fraction).
By gained zymotic fluid through membrane filtration, the zymotic fluid that carbonaceous material is only VFA is obtained, is entered with distilled water mixed diluting
It ferments in next step.
Aerobic fermentation is carried out to gained zymotic fluid in accordance with the following methods：By sub- sieve solution fat yeast liquid (Yarrowia
Lipolytica it) is transferred in 100L fermentation tanks and cultivates according to 10% inoculum concentration, temperature 45 C, speed of agitator 300rpm, ventilation
1.5vvm, pH are controlled 7.0, wherein the culture medium group of 100L fermentation tanks becomes：With 0.22 μm of pore size ceramic membrane filtration sterilization
Zymotic fluid (wherein acetic acid content is about 2.5%) 50kg, pH value 6.8, while flowing and adding the above-mentioned zymotic fluid after filtration sterilization, root
According to data in Fig. 3, the acetic acid content in 100L fermentation tanks is controlled about 0.1~0.4%, when liquid level is more than 80L, flow putting of adding
Material should control liquid level in 80L.It is 3 days that this fermentation process, which uses semicontinuous fermentation mode, period,.
The measurement of dry cell weight：After fermentation broth sample is weighed, 2min is centrifuged with the rotating speed of 4000rpm, gained precipitation adds
Washing is primary, then centrifuges 2min with the rotating speed of 4000rpm, weighs after the drying overnight of direct 80 DEG C of gained precipitation, it is dry to measure cell
Weight is 11.24g/kg zymotic fluids, i.e. every liter of kitchen garbage can produce to obtain about 34.67g yeast.
The measurement of cell grease：After fermentation broth sample is weighed, 2min is centrifuged with the rotating speed of 4000rpm, gained precipitation adds
Washing is primary, then centrifuges 2min with the rotating speed of 4000rpm, and the ether-petroleum ether (body of 4 times of fermentating liquid volumes is added in gained precipitation
Product ratio 1：2), room temperature extracts 2h, and pure water is then added and shakes split-phase, pipettes after organic phase is blown to constant weight with nitrogen and weighs, measure
Cell grease is 1.92g/kg zymotic fluids, accounts for the 38.25% of dry cell weight.
The measurement of cell protein：After fermentation broth sample is weighed, measured in cell using kelvin (Kjeldahl) nitriding
The content of protein, it is 1.63g/kg zymotic fluids to measure cell protein, accounts for the 33.2% of dry cell weight.
The measurement of remaining ferment liquid hold-up：Residue VFA contents are 0.0347g/L in remaining ferment liquid.
Embodiment 3：Using when 35 DEG C of kitchen waste secretly fermentation, 45 DEG C when aerobic two-step fermentation obtain yeast
Anaerobic fermentation is carried out to kitchen garbage in accordance with the following methods：Rubbish from cooking is tentatively sorted, wherein bone is rejected
After the not available substance such as head, hard object, chopsticks and polybag, after centrifugation, dehydration, crushed using food crusher,
Sieving, the rubbish from cooking that can be used for fermenting after handle, wherein total VFA contents are 0.19 ± 0.05g/L, pH value 6.0~
Between 6.5.
It is secretly fermented to kitchen garbage in accordance with the following methods：By kitchen garbage and marine chlorella algae solution in mass ratio 1：
2 ratio is mixed, and (total solids content 6.32%, volatility are solid for the seed sludge of gross mass 8% after then addition mixes
Body content is 2.38%, nitrogen content 0.45%, C/N 4.21, organic component content are 48.81%, salinity 12.0, content with
Wet basis meter), material liquid pH value is adjusted to 7.00 with 1mol/L hydrochloric acid and 1mol/L sodium hydroxides, obtains zymotic fluid.Wherein, algae solution by
Initial concentration is 2 × 1011The marine chlorella addition distilled water of/ml dilutes to obtain, and seed sludge is tamed by fresh anaerobic sludge
After obtain.Acclimation method：It takes fresh anaerobic sludge 100g to be put into 1L conical flasks, kitchen garbage and marine chlorella algae solution is added
Each 4g, continuous addition 5 days, is used in combination magnetic stirring apparatus to be uniformly mixed, is tamed 7 days at 35 ± 1 DEG C, it is about 6.4 or so to obtain pH
Seed sludge.The zymotic fluid is added in fermentation tank, using the air in nitrogen stripping discharge system, forms approximate anaerobism
Environment, controlled at 35 ± 1 DEG C, pH value 6.8 starts to ferment.ORP value controls within the scope of -250~150mV.ORP value
It is measured using ORP meters (PHS-3C, Shanghai thunder magnetic).This fermentation process is using mode of continuously fermenting.
The measurement of VFA：Fermentation broth sample is centrifuged into 20min with the rotating speed of 5000r/min, takes supernatant, it is micro- through 0.45 μm
After the filter membrane of hole, into efficient liquid phase chromatographic analysis, wherein liquid chromatogram selects Shimadzu LC-10AS (UV-VIS SPD-10A types
Detector), mobile phase is 4mm sulfuric acid, flow velocity 0.6ml/min.
Testing result shows that machine object contained in garbage fermentation liquid is mostly VFA, and has a small amount of methane, carbon dioxide and hydrogen
Gas generates.It is 4.26g/L to carry out the available VFA maximum concentrations of anaerobic fermentation to kitchen garbage, and specific VFA yield becomes at any time
Change is shown in that Fig. 4, specific generating rate are shown in Fig. 5.
By gained zymotic fluid through membrane filtration, the zymotic fluid that carbonaceous material is only VFA and a small amount of alcohols material is obtained, with distillation
Water mixed diluting enters to ferment in next step.Aerobic fermentation is carried out to gained zymotic fluid in accordance with the following methods：According to 10% inoculation
Amount, which is transferred in 100L fermentation tanks, cultivates, temperature 45 C, speed of agitator 300rpm, and ventilate 1.5vvm, and pH is controlled 7.2, wherein
The culture medium group of 100L fermentation tanks becomes：With the zymotic fluid of 0.22 μm of pore size ceramic membrane filtration sterilization, (wherein acetic acid content is about
1.2%) 50kg, pH value 6.7, while flowing and adding the zymotic fluid through ceramic membrane filter degerming, middle data, control 100L are sent out according to fig. 3
VFA total contents in fermentation tank are 0.1~0.4%, and when liquid level is more than 80L, liquid level should be controlled in 80L by flowing the blowing added.This hair
Ferment process is using mode of continuously fermenting.
The measurement of dry cell weight：After fermentation broth sample is weighed, 2min is centrifuged with the rotating speed of 4000rpm, gained precipitation adds
Washing is primary, then centrifuges 2min with the rotating speed of 4000rpm, weighs after the drying overnight of direct 80 DEG C of gained precipitation.It is dry to measure cell
Weight is 10.09g/kg zymotic fluids, i.e. every liter of kitchen garbage can produce to obtain about 31.12g yeast.
The measurement of grease：After fermentation broth sample is weighed, 2min, gained precipitation plus washing are centrifuged with the rotating speed of 4000rpm
Once, then with the rotating speed of 4000rpm 2min is centrifuged, the ether-petroleum ether (volume ratio of 4 times of fermentating liquid volumes is added in gained precipitation
1：2), room temperature extracts 2h, and pure water is then added and shakes split-phase, pipettes after organic phase is blown to constant weight with nitrogen and weighs.Measure cell
Grease is 1.55g/kg zymotic fluids, accounts for the 30.3% of dry cell weight.
The measurement of cell protein：After fermentation broth sample is weighed, measured in cell using kelvin (Kjeldahl) nitriding
The content of protein.It is 1.95g/kg zymotic fluids to measure cell protein, accounts for the 38.12% of dry cell weight.
The measurement of remaining ferment liquid hold-up：Remaining ferment liquid component content such as table 2.
Table 2：Fermentation broth contents content after kitchen waste anaerobism, aerobic fermentation
| Ingredient | Content (g/L) |
| VFA | 0.0241 |
| Alcohols material | 0.0035 |
Embodiment 4：Using organic waste through at 35 DEG C secretly fermentation, 28 DEG C when aerobic two-step fermentation obtain microalgae
It is secretly fermented to organic waste in accordance with the following methods：Organic waste is tentatively mixed, heat shock is utilized
Mode pre-processes the mixture of the waste, and the organic waste can be kitchen garbage, Anaerobic Treatment factory row
The waste water and its two kinds or more of mixtures of the sludge, sugared manufacturing industry or paper industry discharge put；It will be processed organic
Waste (pH value is between 5.0~6.0) mixture pours into fermentation tank, is inoculated with anaerobic sludge, contains Mixed Microbes in anaerobic sludge
Group, such as：Clostridium, Enterobacter, genus lactubacillus；Fermentation condition is controlled, is protected from light, temperature is 35~37 DEG C, pH value
It is 6, hydraulic detention time 6h.
The measurement of acetic acid and butyric acid：Fermentation broth sample is centrifuged into 10min with the rotating speed of 5000rpm, supernatant is taken, into height
Effect liquid phase chromatogram is analyzed, wherein liquid chromatogram selects Shimadzu LC-10AS (UV-VIS SPD-10A types detector), and mobile phase is
4mm sulfuric acid, flow velocity 0.6ml/min.
Testing result is shown, after secretly fermenting, machine object contained in garbage fermentation liquid is mostly VFA, and have a small amount of methane,
Carbon dioxide and hydrogen generate.Obtained acetate content can reach 5.2g/L, remaining concrete component content is shown in Table 3.
Table 3：Dark fermentating metabolism product each component content
| Component | Content (g/L) |
| Acetic acid | 5.2 |
| Butyric acid | 2.1 |
| Propionic acid | 1.2 |
| Isovaleric acid | 0.9 |
| Isobutyric acid, valeric acid | <0.5 |
By gained zymotic fluid through membrane filtration, the zymotic fluid that carbonaceous material is only VFA is obtained, is entered with distilled water mixed diluting
It ferments in next step.Microalgae is cultivated in accordance with the following methods：The diluted zymotic fluid is added in micro algae culturing device,
And it is inoculated with marine diatom；Reaction condition, 28 DEG C of temperature are controlled, pH value 9.0 is cultivated.This fermentation process is using continuous hair
Ferment mode.
The detection of microalgae：Using 722S visible spectrophotometers, the OD values of microalgae are measured under 640nm wavelength, through conversion
Obtain microalgae dry weight numerical value.
Testing result is shown, after Heterotrophic culture, obtained microalgae grows into 2.23g/L from 0.67g/L, i.e., every liter has
Machine waste can produce to obtain about 3.133g microalgaes, and microalgae and VFA contents change with time such as Fig. 8, and VFA end contents are
0.0393g/L.It can be seen that reduction of the increase of microalgae content directly along with VFA contents, and the fermentation after membrane filtration
In liquid, carbonaceous material is only VFA.Wherein, oil content rises to 37.1% from 20.25%.
Claims (10)
1. a kind of microbial fermentation or microbe preparation method, which is characterized in that the method includes：
(1) to carrying out anaerobic fermentation or dark fermentation suitable for the rubbish of fermentation, zymotic fluid is obtained；
(2) zymotic fluid obtained to step (1) is separated by solid-liquid separation；With
(3) aerobic fermentation is carried out as substrate using the zymotic fluid that step (2) obtains.
2. the method as described in claim 1, which is characterized in that
The rubbish derives from organic garbage of city, is solid waste, liquid debris and their mixture；It is preferred that
Ground, the solid waste are selected from：Castoff, kitchen garbage or combination thereof；And/or
The rubbish for being suitable for fermentation described in step (1) is to pass through pretreated rubbish, and grain size is less than 2mm, and pH is 6.00~6.50
Between, preferably between 6.20~6.30, more preferably 6.25 ± 0.03；Preferably, the pre-treatment step includes：By rubbish
It is sieved with 2mm sieves after broken, and sieving is prevented from being acidified at being placed at 5 DEG C of temperature below.
3. the method as described in any one of claim 1-2, which is characterized in that carry out detesting for step (1) under the following conditions
Aerobe fermentation or dark fermentation：
Inoculum concentration：8~20%, such as 8~10%, 10~15% or 8~15%；
Fermentation temperature：20~55 DEG C, such as 25~35 DEG C or 28~32 DEG C；With
pH：6.0~8.0, such as 6.8~7.2.
4. method as claimed in any one of claims 1-3, which is characterized in that
Using zymogenic bacteria, hydrogen-producing acetogens, consumption hydrogen acetogen or its arbitrary combination to the rubbish progress suitable for fermentation
Anaerobic fermentation；Or
Anaerobic fermentation is carried out using or mixtures thereof yeast, acetic acid bacteria；Preferably, the yeast is saccharomyces cerevisiae, the acetic acid
Bacterium is brewing acetic acid bacteria；Or
Anaerobic fermentation is carried out using the mixed bacterial contained by anaerobic sludge；Preferably, the anaerobic sludge is to come from Treatment of Sludge
The anaerobic sludge that the anaerobic fermentation of kitchen waste system of factory generates；Preferably, the mixed bacterial contained by the anaerobic sludge contains
Bacterium from Clostridium, Enterobacter and genus lactubacillus；Or
Utilize the fungal component selected from hydrogen-producing acetogens (such as Desulfotomaculum sp.Iso-W2), hydrogen-producing acetogens
Bacterium (such as Clostridium of (such as Sedimentibacter sp.JN18-A14-H), degradable amino acid
Hydroxybenzoicum) or their combination of two or more carries out anaerobic fermentation；Or
It is secretly fermented using algae；Preferably, the algae is selected from Scenedesmus quadricauda, scenedesmus obliquus, marine diatom, micro- quasi- ball algae
With it is one or more in chlorella.
5. the method as described in any one of claim 1-4, which is characterized in that
Organic acid contained in the zymotic fluid obtained through anaerobic fermentation in step (1) be volatile organic acids, be preferably selected from formic acid,
One kind in acetic acid, propionic acid, lactic acid and butyric acid or arbitrary a variety of combination；
Preferably, in the organic substance of the zymotic fluid obtained through anaerobic fermentation in step (1), 80% or more is volatile fat
Acid；
Preferably, the pH of the zymotic fluid obtained through anaerobic fermentation in step (1) in the range of 4.0~9.0, such as 5.5~
6.5,6.5~7.0 or 6.5~7.9.
6. the method as described in any one of claim 1-5, which is characterized in that
The separation of solid and liquid of step (2) is carried out using filter membrane；
Preferably, in the zymotic fluid obtained after separation of solid and liquid, carbonaceous material is volatile organic acids and alcohols material, it is preferable that
Carbonaceous material is formic acid, acetic acid, propionic acid, isobutyric acid, valeric acid, isovaleric acid, methanol, one kind of ethyl alcohol or arbitrary a variety of combination.
7. the method as described in any one of claim 1-6, which is characterized in that
Using selected from microalgae, yeast, Purple Nonsulfer Bacteria, the microorganism of mould or their two or more of combinations
Carry out the aerobic fermentation described in step (3)；
Preferably, the yeast is selected from：Shallow white Cryptococcus (Cryptococcus albidus), curved Cryptococcus
(Cryptococcus albidun), Lipomyces starkeyi (Lipomyces starkeyi), Trichosporon pullulans
(Trichospiron pullulans), oil-producing saccharomyces oleaginosus (Lipomyces lipofer), Rhodotorula glutinus
(Rhodotorula giutinis), the red winter spore of class yeast (Rhodosporidium toruloides), sub- sieve solution fat yeast
(Yarrowia lipolytica), candida utili (Candida utilis), candida tropicalis (Candida
Tropicalis), garden false yeast (Torula utilis), torulopsis (Torulopsis utilis), saccharomyces cerevisiae
(Saccharomyces cerevisiae), sub- sieve solution fat yeast (Yarrowia lipolytica) or their two kinds or more
The combination of kind；
The Purple Nonsulfer Bacteria is selected from：Rhodopseudomonas palustris (Rhodopseudomonas palustris), class ball
Red pseudomonas (Rhodobacter sphaeroides), rhodopseudomonas acidophilus (Rhodopseudomonas
Acidophilus), capsula Rhodopseudomonas (Rhodopseudomonas capsulata), red spirillum
(Rhodospirillum) or their two or more of combinations；
The mould is selected from：Native mould (Asoergullus terreus), purple paralysis ergot (Clavicepspurpurea) are high
Fine strain of millet pleat embraces smut (Tolyposporium), Mortierella alpina (Mortierella alpina), Mortierella isabellina
(Mortierrella isabellina) or their two or more of combinations；
The microalgae is selected from Scenedesmus quadricauda, scenedesmus obliquus, marine diatom, micro- quasi- ball algae, chlorella or they two or more
Combination.
8. the method for claim 7, which is characterized in that the aerobic fermentation is implemented as follows：
Inoculum concentration is usually 1~30%, such as 5~25% either 5~20% or 5~15%；
The content of initial organic acid in fermentation tank is controlled in the range of 1~5%, preferably 2~4%；
During the fermentation, the organic acid content in fermentation tank can be controlled to the model 0.1~1.0%, such as 0.1~0.5%
In enclosing；
Fermentation temperature is usually in the range of 25~50 DEG C, for example, 28~45 DEG C, 30~45 DEG C or 40~50 DEG C；With
Preferably, pH controls are in the range of 7.0 ± 0.3；
Preferably, ventilatory capacity control is in 0.5~2.0vvm；
Preferably, speed of agitator control is in the range of 150~500rpm.
9. the method as described in any one of claim 1-8, which is characterized in that the anaerobic fermentation, dark fermentation and aerobic hair
Ferment is batch fermentation, semicontinuous fermentation or continuously ferments, and for semicontinuous fermentation and is continuously fermented, the mode of feed supplement to ferment
Organic acid content in tank controls in the range of 0~0.5%, such as controls 0.05~0.5% or 0.1~0.4%,
Either 0.1~0.3% either in the range of 0.2~0.5% or 0.2~0.4%.
10. a kind of waste disposal method, which is characterized in that the method includes implementing described in any one of claim 1-9
The step of microbial fermentation processes.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| CN114790126A (en) * | 2021-05-11 | 2022-07-26 | 上海柯珑清洁技术有限公司 | Anaerobic fermentation treatment process for kitchen waste and application thereof |
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| CN116555356A (en) * | 2023-04-12 | 2023-08-08 | 西北农林科技大学深圳研究院 | Method for promoting anaerobic digestion to produce hydrogen alkane by using lactic acid bacteria |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110408553A (en) * | 2019-08-06 | 2019-11-05 | 苏州吉态来胺生物科技有限公司 | A method of single cell protein is produced by raw material of palm waste |
| CN111057656A (en) * | 2019-12-02 | 2020-04-24 | 轻工业环境保护研究所 | Yeast for efficiently degrading waste liquid in ice cream production and application thereof |
| CN111057656B (en) * | 2019-12-02 | 2021-11-09 | 轻工业环境保护研究所 | Yeast for efficiently degrading waste liquid in ice cream production and application thereof |
| CN114790126A (en) * | 2021-05-11 | 2022-07-26 | 上海柯珑清洁技术有限公司 | Anaerobic fermentation treatment process for kitchen waste and application thereof |
| CN114790126B (en) * | 2021-05-11 | 2023-09-15 | 上海柯珑清洁技术有限公司 | Anaerobic fermentation treatment process for kitchen waste and application of anaerobic fermentation treatment process |
| CN113801797A (en) * | 2021-07-30 | 2021-12-17 | 江苏苏星资产管理有限公司 | High-enzyme-activity functional microbial agent and preparation method and application thereof |
| CN113801797B (en) * | 2021-07-30 | 2023-10-31 | 江苏苏星资产管理有限公司 | High-enzyme-activity functional microbial agent and preparation method and application thereof |
| CN114807251A (en) * | 2022-05-08 | 2022-07-29 | 北京工业大学 | Method for strengthening acid production by co-digestion of kitchen waste and activated sludge through freezing/temperature pretreatment |
| CN116555356A (en) * | 2023-04-12 | 2023-08-08 | 西北农林科技大学深圳研究院 | Method for promoting anaerobic digestion to produce hydrogen alkane by using lactic acid bacteria |
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