CN108779469A - 用以建构转位子的套组及其用途 - Google Patents

用以建构转位子的套组及其用途 Download PDF

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Publication number
CN108779469A
CN108779469A CN201680082512.4A CN201680082512A CN108779469A CN 108779469 A CN108779469 A CN 108779469A CN 201680082512 A CN201680082512 A CN 201680082512A CN 108779469 A CN108779469 A CN 108779469A
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CN
China
Prior art keywords
terminal repeat
promoter
gene
sequence
cell
Prior art date
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Pending
Application number
CN201680082512.4A
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English (en)
Chinese (zh)
Inventor
吴琼媛
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pioneer Biotechnology Co.,Ltd.
Original Assignee
Genome Pioneer Medical Ltd By Share Ltd
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Application filed by Genome Pioneer Medical Ltd By Share Ltd filed Critical Genome Pioneer Medical Ltd By Share Ltd
Publication of CN108779469A publication Critical patent/CN108779469A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/90—Vectors containing a transposable element

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201680082512.4A 2016-03-25 2016-03-25 用以建构转位子的套组及其用途 Pending CN108779469A (zh)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2016/077323 WO2017161553A1 (en) 2016-03-25 2016-03-25 Kit for constructing transposon and use thereof

Publications (1)

Publication Number Publication Date
CN108779469A true CN108779469A (zh) 2018-11-09

Family

ID=59899851

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201680082512.4A Pending CN108779469A (zh) 2016-03-25 2016-03-25 用以建构转位子的套组及其用途

Country Status (4)

Country Link
US (1) US11299748B2 (de)
EP (1) EP3433366B1 (de)
CN (1) CN108779469A (de)
WO (1) WO2017161553A1 (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10704021B2 (en) 2012-03-15 2020-07-07 Flodesign Sonics, Inc. Acoustic perfusion devices
CN105939767B (zh) 2014-01-08 2018-04-06 弗洛设计声能学公司 具有双声电泳腔的声电泳装置
US11377651B2 (en) 2016-10-19 2022-07-05 Flodesign Sonics, Inc. Cell therapy processes utilizing acoustophoresis
US11708572B2 (en) 2015-04-29 2023-07-25 Flodesign Sonics, Inc. Acoustic cell separation techniques and processes
US11214789B2 (en) 2016-05-03 2022-01-04 Flodesign Sonics, Inc. Concentration and washing of particles with acoustics
US20190119636A1 (en) 2017-10-23 2019-04-25 Poseida Therapeutics, Inc. Modified stem cell memory t cells, methods of making and methods of using same
CA3085784A1 (en) 2017-12-14 2019-06-20 Flodesign Sonics, Inc. Acoustic transducer driver and controller

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7063947B2 (en) * 2004-04-08 2006-06-20 Promogen, Inc. System for producing synthetic promoters
WO2006122442A1 (en) * 2005-05-14 2006-11-23 Fudan University Piggybac as a tool for genetic manipulation and analysis in vertebrates
US10398787B2 (en) * 2012-10-26 2019-09-03 Vrije Universiteit Brussel Vectors for liver-directed gene therapy of hemophilia and methods and use thereof
CN103834686B (zh) * 2012-11-23 2016-05-25 上海药明生物技术有限公司 高效克隆筛选表达载体、其制备方法及用途

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
IVANA GRABUNDZIJA等: "Comparative Analysis of Transposable Element Vector Systems in Human Cells", 《MOL THER.》 *
LANDREMAN,A.等: "GenBank: KM359772.1-Cloning vector pNLCoI2[luc2-P2A-NlucP/minP/Hygro], complete sequence", 《GENBANK》 *
SHAO,H.等: "GenBank: JX013636.1-PiggyBac_based_Ss_Act2_gfp_era1 vector pPV254, complete sequence", 《GENBANK》 *
SUNANDAN SAHA等: "Evaluating the potential for undesired genomic effects of the piggyBac transposon system in human cells", 《NUCLEIC ACIDS RESEARCH》 *
VALERI V. MOSSINE等: "piggyBac Transposon plus Insulators Overcome Epigenetic Silencing to Provide for Stable Signaling Pathway Reporter Cell Lines", 《PLOS》 *

Also Published As

Publication number Publication date
EP3433366A4 (de) 2019-08-28
US20190040414A1 (en) 2019-02-07
WO2017161553A1 (en) 2017-09-28
EP3433366B1 (de) 2022-05-18
EP3433366A1 (de) 2019-01-30
US11299748B2 (en) 2022-04-12

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Address after: England group

Applicant after: Pioneer Biotechnology Co.,Ltd.

Address before: England group

Applicant before: GenomeFrontier Therapeutics, Inc.

TA01 Transfer of patent application right
TA01 Transfer of patent application right

Effective date of registration: 20210108

Address after: Taipei City, Taiwan, China

Applicant after: Pioneer Biotechnology Co.,Ltd.

Address before: England group

Applicant before: Pioneer Biotechnology Co.,Ltd.

WD01 Invention patent application deemed withdrawn after publication
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20181109