CN108773831A - L-arginine nano-particle and the degradable nano-motor and preparation method thereof that nitric oxide is power source - Google Patents
L-arginine nano-particle and the degradable nano-motor and preparation method thereof that nitric oxide is power source Download PDFInfo
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- CN108773831A CN108773831A CN201810595299.8A CN201810595299A CN108773831A CN 108773831 A CN108773831 A CN 108773831A CN 201810595299 A CN201810595299 A CN 201810595299A CN 108773831 A CN108773831 A CN 108773831A
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- arginine
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- nitric oxide
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- ODKSFYDXXFIFQN-BYPYZUCNSA-N L-arginine Chemical compound OC(=O)[C@@H](N)CCCN=C(N)N ODKSFYDXXFIFQN-BYPYZUCNSA-N 0.000 title claims abstract description 110
- 229930064664 L-arginine Natural products 0.000 title claims abstract description 110
- 235000014852 L-arginine Nutrition 0.000 title claims abstract description 110
- MWUXSHHQAYIFBG-UHFFFAOYSA-N Nitric oxide Chemical compound O=[N] MWUXSHHQAYIFBG-UHFFFAOYSA-N 0.000 title claims abstract description 71
- 239000002105 nanoparticle Substances 0.000 title claims abstract description 71
- 238000002360 preparation method Methods 0.000 title claims abstract description 16
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims abstract description 57
- 239000004952 Polyamide Substances 0.000 claims abstract description 44
- 229920002647 polyamide Polymers 0.000 claims abstract description 44
- 230000033001 locomotion Effects 0.000 claims abstract description 22
- 239000000126 substance Substances 0.000 claims abstract description 20
- 125000000524 functional group Chemical group 0.000 claims abstract description 12
- 239000000446 fuel Substances 0.000 claims abstract description 9
- 238000006479 redox reaction Methods 0.000 claims abstract description 9
- 238000001338 self-assembly Methods 0.000 claims abstract description 4
- 229960002163 hydrogen peroxide Drugs 0.000 claims description 27
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- 239000013049 sediment Substances 0.000 claims description 9
- 108010039918 Polylysine Proteins 0.000 claims description 6
- 229920000656 polylysine Polymers 0.000 claims description 6
- 239000004475 Arginine Substances 0.000 claims description 4
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims description 4
- 235000009697 arginine Nutrition 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- 229920001661 Chitosan Polymers 0.000 claims description 3
- 238000004821 distillation Methods 0.000 claims description 2
- 238000005406 washing Methods 0.000 claims description 2
- 230000005611 electricity Effects 0.000 claims 1
- 239000000243 solution Substances 0.000 description 37
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 12
- 230000015572 biosynthetic process Effects 0.000 description 8
- 235000013339 cereals Nutrition 0.000 description 8
- 238000005119 centrifugation Methods 0.000 description 7
- 238000006731 degradation reaction Methods 0.000 description 7
- 239000012153 distilled water Substances 0.000 description 7
- 239000007789 gas Substances 0.000 description 7
- 238000000034 method Methods 0.000 description 7
- 238000002604 ultrasonography Methods 0.000 description 7
- 229910021529 ammonia Inorganic materials 0.000 description 6
- 238000001035 drying Methods 0.000 description 6
- 238000003786 synthesis reaction Methods 0.000 description 6
- 230000015556 catabolic process Effects 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 5
- 229920002101 Chitin Polymers 0.000 description 4
- 239000002253 acid Substances 0.000 description 4
- 230000008859 change Effects 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 239000011521 glass Substances 0.000 description 4
- 239000001257 hydrogen Substances 0.000 description 4
- 229910052739 hydrogen Inorganic materials 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 3
- 239000006227 byproduct Substances 0.000 description 3
- DWNBOPVKNPVNQG-LURJTMIESA-N (2s)-4-hydroxy-2-(propylamino)butanoic acid Chemical compound CCCN[C@H](C(O)=O)CCO DWNBOPVKNPVNQG-LURJTMIESA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- OAKJQQAXSVQMHS-UHFFFAOYSA-N Hydrazine Chemical compound NN OAKJQQAXSVQMHS-UHFFFAOYSA-N 0.000 description 2
- 241000209094 Oryza Species 0.000 description 2
- 235000007164 Oryza sativa Nutrition 0.000 description 2
- 150000001408 amides Chemical class 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 239000003054 catalyst Substances 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 230000007850 degeneration Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000000686 essence Substances 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- NUJOXMJBOLGQSY-UHFFFAOYSA-N manganese dioxide Chemical compound O=[Mn]=O NUJOXMJBOLGQSY-UHFFFAOYSA-N 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 150000002978 peroxides Chemical class 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 235000009566 rice Nutrition 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 210000001541 thymus gland Anatomy 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- CPELXLSAUQHCOX-UHFFFAOYSA-M Bromide Chemical compound [Br-] CPELXLSAUQHCOX-UHFFFAOYSA-M 0.000 description 1
- WKBOTKDWSSQWDR-UHFFFAOYSA-N Bromine atom Chemical compound [Br] WKBOTKDWSSQWDR-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 1
- 108090000913 Nitrate Reductases Proteins 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 229940006460 bromide ion Drugs 0.000 description 1
- GDTBXPJZTBHREO-UHFFFAOYSA-N bromine Substances BrBr GDTBXPJZTBHREO-UHFFFAOYSA-N 0.000 description 1
- 229910052794 bromium Inorganic materials 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000005684 electric field Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 150000002431 hydrogen Chemical class 0.000 description 1
- XMBWDFGMSWQBCA-UHFFFAOYSA-N hydrogen iodide Chemical compound I XMBWDFGMSWQBCA-UHFFFAOYSA-N 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000002519 immonomodulatory effect Effects 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 230000004199 lung function Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 230000008470 skin growth Effects 0.000 description 1
- 210000000329 smooth muscle myocyte Anatomy 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 238000010189 synthetic method Methods 0.000 description 1
- 210000004367 thymic lymphocyte Anatomy 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82B—NANOSTRUCTURES FORMED BY MANIPULATION OF INDIVIDUAL ATOMS, MOLECULES, OR LIMITED COLLECTIONS OF ATOMS OR MOLECULES AS DISCRETE UNITS; MANUFACTURE OR TREATMENT THEREOF
- B82B1/00—Nanostructures formed by manipulation of individual atoms or molecules, or limited collections of atoms or molecules as discrete units
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82B—NANOSTRUCTURES FORMED BY MANIPULATION OF INDIVIDUAL ATOMS, MOLECULES, OR LIMITED COLLECTIONS OF ATOMS OR MOLECULES AS DISCRETE UNITS; MANUFACTURE OR TREATMENT THEREOF
- B82B3/00—Manufacture or treatment of nanostructures by manipulation of individual atoms or molecules, or limited collections of atoms or molecules as discrete units
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y40/00—Manufacture or treatment of nanostructures
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
- C08K5/00—Use of organic ingredients
- C08K5/16—Nitrogen-containing compounds
- C08K5/29—Compounds containing one or more carbon-to-nitrogen double bonds
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- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Nanotechnology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Manufacturing & Machinery (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Condensed Matter Physics & Semiconductors (AREA)
- General Physics & Mathematics (AREA)
- Polymers & Plastics (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Processes Of Treating Macromolecular Substances (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention discloses the degradable nano-motor and preparation method thereof that a kind of L-arginine nano-particle and nitric oxide are power source, which is self-assembly of with the substance for being rich in electropositive functional group ultrabranching polyamide by electrostatical binding by L-arginine；Using L-arginine nano-particle as fuel, degradable nano-motor can be formed by power source of nitric oxide.The present invention releases nitric oxide bubble using the redox reaction of L-arginine and hydrogen peroxide, thus nano-particle movement is pushed, becomes nano-motor, and with the consumption of L-arginine, L-arginine nano-particle is gradually degraded, and being finally degraded to water-soluble substances can be excreted by human body.
Description
Technical field
The invention belongs to novel biomaterials, and in particular to L-arginine nano-particle and nitric oxide be power source can
Degradation-type nano-motor and preparation method thereof.
Background technology
With the development of science and technology, nanotechnology and material science have also been made significant headway.Nano science gradually starts
By materialogy, physics, biology, electronics educational circles numerous researchers extensive concern, become an important friendship
Pitch subject.People are inspired by molecular motor, copy the operation principle of molecular motor to prepare using nanoscale science and technology means
Nano-motor is gone out.Existing nano-motor can be divided into the three categories such as rodlike motor, tubular motor, spherical motor from shape；
Also include some motors in irregular shape simultaneously, such as dish type, spiral shape, cryptomere, tubaeform.From power source can be divided into
Lower two major classes：One kind is extraneous physical stimulation driving, usually there is magnetic field, electric field, ultrasonic wave, illumination etc.；Another kind of is chemical energy
Source drives, and typically water, hydrogen peroxide, acid solution, hydrazine, urea, bromine and iodide etc. are used as fuel, by catalyst and fuel
Reaction generates the movement that the gases such as hydrogen, oxygen, ammonia push nano-motor as power source.
The power source and the product in reaction process of the nano-motor of current research are mostly the unwanted exhaust gas of human body
(such as ammonia, hydrogen) or waste material (such as bromide ion), and most of catalyst can not degrade in vivo, residual causes human body in vivo
Burden.Mariana Medina-Sanchez in 2017 et al. are published in an article on Nature and propose current nano-motor
Research needs emphasis to consider the nano-motor that can control, stop and remove, and does not have the consumption that can utilize material itself also at present
And realize the report of the degradation of nano-motor.Therefore, there is an urgent need for exploitation prepare it is degradable, using useful constituent as power source, generate
Beneficial to human body, the degradable novel nano motor of by-product.
Invention content
Goal of the invention：In view of the problems of the existing technology, the present invention provides a kind of L-arginine nano-particle, the nanometer
Particle can by the consumption of material itself realize formation nano-motor degradation.
The present invention also provides the degradable nano-motors and preparation method thereof that a kind of nitric oxide is power source.
Technical solution：To achieve the goals above, a kind of L-arginine nano-particle as described herein, by L- essence ammonia
The nano-particle that acid is formed with the substance rich in electropositive functional group.
Preferably, the substance rich in electropositive functional group is the ultrabranching polyamide or poly- bad in two generations or more
Propylhomoserin or chitosan.
The preparation method of L-arginine nano-particle of the present invention by L-arginine and is rich in electropositive functional group
Substance is configured to solution respectively, and after the two ultrasonic mixing, centrifuging and taking lower sediment distillation washing, drying are made.
Preferably, a concentration of 2.5-15mg/mL of the L-arginine solution.
Preferably, a concentration of 0.1-1mg/mL of the substance solution rich in electropositive functional group.
Wherein, the L-arginine presses 1-10mLL- arginine solutions and 1-10mL with the substance rich in electropositive functional group
Substance solution ultrasonic mixing 1-60min rich in electropositive functional group.
It is of the present invention using nitric oxide as the degradable nano-motor of power source, with L-arginine nano-particle and mistake
The nitric oxide that hydrogen oxide reaction generates is power source, is formed by fuel of hydrogen peroxide.
Nitric oxide of the present invention is the preparation method of the degradable nano-motor of power source, is received by L-arginine
The redox reaction of rice corpuscles and hydrogen peroxide releases nitric oxide bubble, pushes nano-particle movement, becomes a nanometer horse
It reaches, with the consumption of L-arginine, L-arginine nano-particle is gradually degraded, and water-soluble substances are finally degraded to.
Specially L-arginine/ultrabranching polyamide nano-particle is added dropwise and is dried on glass slide, hydrogen peroxide is added dropwise
Solution is placed in observation motor movement situation on microscope.The redox reaction of L-arginine and hydrogen peroxide releases an oxygen
Change nitrogen bubble, thus pushes nano-particle movement, formed using nitric oxide as the degradable nano-motor of power source.
Wherein, the mass concentration of the hydrogenperoxide steam generator is 5-20%.
The present invention is with the degradable nano-motor (grain size 50-200nm) of L-arginine nano-particle, by electropositive base
The abundant substance of group is obtained with L-arginine by way of electrostatical binding self assembly.
Mechanism：The present invention is using hydrogen peroxide as fuel, with L-arginine nano-particle and the one of hydroperoxidation generation
Nitrogen oxide is the degradable nano-motor of power source in application, being released using the redox reaction of L-arginine and hydrogen peroxide
Nitric oxide bubble is released, nano-particle movement is thus pushed, becomes nano-motor, and with the consumption of L-arginine, L- essences
Propylhomoserin nano-particle is gradually degraded, and being finally degraded to water-soluble substances can be excreted by human body.Nitric oxide has in promotion
Skin growth, migration, adjusts the effects that collage synthesis, other cell factors at the proliferation for inhibiting smooth muscle cell；L-arginine then has
There is immunoloregulation function, can prevent the degeneration (degeneration especially after injury) of thymus gland, supplement arginine from can increase thymus gland
Weight, promote the growth of thymic lymphocytes；Citrulling can then improve function of immune system, safeguard joint motions function,
Balance normal blood glucose level, harmful free radical absorbed containing abundant antioxidant, the normal level for assisting in keeping cholesterol,
It safeguards the lung function in Jiankang, can also be acted on ammonia in human body, generate arginine and nitric oxide.Either nano-motor
The by-product citrulling that raw material L-arginine, power source nitric oxide still react is required important substance in human body, because
This synthetic method provided by the invention has broad application prospects in biomedical sector.
Advantageous effect：Compared with prior art, the invention has the advantages that：
The present invention synthesizes L-arginine nano-particle using the method for electrostatical binding self assembly, utilizes L-arginine and peroxide
The redox reaction for changing hydrogen releases nitric oxide bubble, thus pushes nano-particle movement, becomes nano-motor, while with
The consumption of L-arginine, L-arginine nano-particle is gradually degraded, and water-soluble substances are finally degraded to.Nanometer of the present invention
Particle can by the consumption of material itself realize formation nano-motor degradation, and preparation manipulation method is simple, closes
At mild condition, nano-motor is generated without exhaust gas, waste material during the motion, and raw material L-arginine, the power of nano-motor
Source nitric oxide and the by-product citrulling of reaction are required important substance in human body, are had in biomedical sector wide
Application prospect.
Description of the drawings
Fig. 1 is the TEM figures for L-arginine/ultrabranching polyamide nano-particle that embodiment 1 obtains；
Fig. 2 is the TEM figures for L-arginine/ultrabranching polyamide nano-particle that embodiment 2 obtains；
Fig. 3 is the TEM figures for L-arginine/ultrabranching polyamide nano-particle that embodiment 3 obtains；
Fig. 4 is the TEM figures for L-arginine/polylysine nano-particle that embodiment 4 obtains；
Fig. 5 is the TEM figures for L-arginine/chitin nanometer that embodiment 5 obtains；
Fig. 6 is sport video sectional drawing of the L-arginine/ultrabranching polyamide nano-particle in 20% hydrogenperoxide steam generator：
(A) the moving line figure in the motion state of different time and (B) 10s；
Fig. 7 is sport video sectional drawing of the L-arginine/ultrabranching polyamide nano-particle in 10% hydrogenperoxide steam generator：
(A) the moving line figure in the motion state of different time and (B) 10s；
Fig. 8 is sport video sectional drawing of the L-arginine/ultrabranching polyamide nano-particle in 5% hydrogenperoxide steam generator：
(A) the moving line figure in the motion state of different time and (B) 10s；
Fig. 9 is the obtained L-arginine/ultrabranching polyamide nano-particle of embodiment 1 in 10%H2O2In NO discharge feelings
Condition；
Figure 10 is that embodiment 1 obtains L-arginine/ultrabranching polyamide nano-particle in 10%H2O2In degradation situation,
(a)0h,(b)1h,(c)3h,(d)6h,(e)18h。
Specific implementation mode
Embodiment 1
The synthesis of L-arginine/ultrabranching polyamide nano-particle：
(1) L-arginine (L-Arg) solution soluble in water for being made into a concentration of 5mg/mL is weighed；
(2) solution that a certain amount of ultrabranching polyamide (HPAM) is made into a concentration of 0.5mg/mL is weighed.
(3) the L-arginine solution of the above-mentioned configurations of 1mL is taken, ultrasound, and the hyperbranched of 0.5mg/mL is added dropwise thereto
Polyamide solution 1mL.After continual ultrasonic 5min, 12000rpm/min centrifugations, lower sediment distilled water washes twice drying, obtains
L-arginine/ultrabranching polyamide nano-particle is for use.L-arginine/ultrabranching polyamide nanoparticle prepared by the present embodiment 1
The TEM of son schemes to have synthesized L-arginine/hyperbranched poly that grain size is about 130nm by this method as shown in Figure 1, can be seen that in figure
Amide nano-particle, with relatively regular geometric shape.
Embodiment 2
The synthesis of L-arginine/ultrabranching polyamide nano-particle：
(1) L-arginine (L-Arg) solution soluble in water for being made into a concentration of 2.5mg/mL is weighed；
(2) solution that a certain amount of ultrabranching polyamide (HPAM) is made into a concentration of 0.5mg/mL is weighed.
(3) the L-arginine solution of the above-mentioned configurations of 1mL is taken, ultrasound, and the hyperbranched of 0.5mg/mL is added dropwise thereto
Polyamide solution 1mL.After continual ultrasonic 5min, 12000rpm/min centrifugations, lower sediment distilled water washes twice dried for standby.
The TEM of L-arginine/ultrabranching polyamide nano-particle prepared by the present embodiment 2 schemes as shown in Fig. 2, can be seen that through this in figure
Method has synthesized L-arginine/ultrabranching polyamide nano-particle that grain size is about 100nm.
Embodiment 3
The synthesis of L-arginine/ultrabranching polyamide nano-particle：
(1) L-arginine (L-Arg) solution soluble in water for being made into a concentration of 15mg/mL is weighed；
(2) solution of a certain amount of ultrabranching polyamide (HPAM) at a concentration of 0.5mg/mL is weighed.
(3) the L-arginine solution of the above-mentioned configurations of 1mL is taken, ultrasound, and the hyperbranched of 0.5mg/mL is added dropwise thereto
Polyamide (HPAM) 1mL.After continual ultrasonic 5min, 12000rpm/min centrifugations, lower sediment distilled water washes twice drying and waits for
With.The TEM of L-arginine/ultrabranching polyamide nano-particle prepared by the present embodiment 3 schemes as shown in figure 3, with L-arginine
The grain size of the increase of concentration, L-arginine/ultrabranching polyamide nano-particle gradually increases, when L-arginine concentration reaches
When 15mg/mL, grain size has been more than 500 μm, and apparent crystalline polamer occurs.
Embodiment 4
The synthesis of L-arginine/polylysine nano-particle：
(1) L-arginine (L-Arg) solution soluble in water for being made into a concentration of 5mg/mL is weighed；
(2) solution that a certain amount of polylysine is made into a concentration of 0.5mg/mL is weighed.
(3) the L-arginine solution of the above-mentioned configurations of 1mL is taken, ultrasound, and the poly- bad ammonia of 0.5mg/mL is added dropwise thereto
Acid solution 1mL.After continual ultrasonic 5min, 12000rpm/min centrifugations, lower sediment distilled water washes twice dried for standby.This reality
The TEM figures for applying L-arginine/polylysine nano-particle of the preparation of example 4 are synthesized as shown in figure 4, can be seen that in figure by this method
L-arginine/polylysine nano-particle that grain size is about 800nm.
Embodiment 5
The synthesis of L-arginine/chitin nanometer：
(1) L-arginine (L-Arg) solution soluble in water for being made into a concentration of 5mg/mL is weighed；
(2) solution that a certain amount of chitosan is made into a concentration of 0.5mg/mL is weighed.
(3) the L-arginine solution of the above-mentioned configurations of 1mL is taken, ultrasound, and the poly- bad ammonia of 0.5mg/mL is added dropwise thereto
Acid solution 1mL.After continual ultrasonic 5min, 12000rpm/min centrifugations, lower sediment distilled water washes twice dried for standby.This reality
The TEM figures for applying L-arginine/chitin nanometer of the preparation of example 5 are synthesized as shown in figure 5, can be seen that in figure by this method
L-arginine/chitin nanometer that grain size is about 200-500nm.
Embodiment 6
(1) L-arginine (L-Arg) solution soluble in water for being made into a concentration of 2.5mg/mL is weighed；
(2) solution that a certain amount of ultrabranching polyamide (HPAM) is made into a concentration of 0.1mg/mL is weighed.
(3) the L-arginine solution of the above-mentioned configurations of 5mL is taken, ultrasound, and the hyperbranched of 0.1mg/mL is added dropwise thereto
Polyamide solution 5mL.After continual ultrasonic 1min, 12000rpm/min centrifugations, lower sediment distilled water washes twice dried for standby.
Embodiment 7
(1) L-arginine (L-Arg) solution soluble in water for being made into a concentration of 2.5mg/mL is weighed；
(2) solution that a certain amount of ultrabranching polyamide (HPAM) is made into a concentration of 1mg/mL is weighed.
(3) the L-arginine solution of the above-mentioned configurations of 10mL is taken, ultrasound, and the hyperbranched poly of 1mg/mL is added dropwise thereto
Amide solution 10mL.After continual ultrasonic 60min, 12000rpm/min centrifugations, lower sediment distilled water washes twice dried for standby.
Embodiment 8
Using nitric oxide as the degradable nano-motor of power source：
L-arginine prepared by embodiment 1/ultrabranching polyamide nano-particle is added dropwise on glass slide, in 37 DEG C of baking ovens
Middle drying.The hydrogenperoxide steam generator that mass concentration is 20% is added dropwise, is placed in observation motor movement situation on microscope.L-arginine
The redox reaction of nano-particle and hydrogen peroxide releases nitric oxide bubble, thus pushes nano-particle movement, is formed
Using nitric oxide as power source, using hydrogen peroxide as the degradable nano-motor of fuel.
As shown in fig. 6, L-arginine/ultrabranching polyamide nano-particle is in 20% hydrogenperoxide steam generator of mass concentration
Motion state by can be seen that the motor energy continuous uniform generates NO gases and moving line is linear in figure, and moves speed
Rate is very fast.
Embodiment 9
Using nitric oxide as the degradable nano-motor of power source：
L-arginine prepared by embodiment 1/ultrabranching polyamide nano-particle is added dropwise on glass slide, in 37 DEG C of baking ovens
Middle drying.The hydrogenperoxide steam generator that mass concentration is 10% is added dropwise, is placed in observation L-arginine/ultrabranching polyamide on microscope
The motion conditions of nano-particle.The redox reaction of L-arginine nano-particle and hydrogen peroxide releases nitric oxide gas
Thus bubble pushes nano-particle movement, is formed using nitric oxide as power source, using hydrogen peroxide as the degradable nanometer of fuel
Motor.
As shown in fig. 7, L-arginine/ultrabranching polyamide nano-particle is in 10% hydrogenperoxide steam generator of mass concentration
Motion state is in circle type by can be seen that the motor energy continuous uniform generates NO gases and moving line in figure, this and over-expense
It is related it to be loaded with asymmetric L-arginine unbalance stress when it is with hydroperoxidation in change polyamide cavity.
Embodiment 10
Using nitric oxide as the degradable nano-motor of power source：
L-arginine prepared by embodiment 1/ultrabranching polyamide nano-particle is added dropwise on glass slide, in 37 DEG C of baking ovens
Middle drying.The hydrogenperoxide steam generator that mass concentration is 5% is added dropwise, is placed in observation L-arginine/ultrabranching polyamide on microscope
Nano-particle motion conditions.The redox reaction of L-arginine nano-particle and hydrogen peroxide releases nitric oxide bubble,
Thus nano-particle movement is pushed, is formed using nitric oxide as power source, using hydrogen peroxide as the degradable nanometer horse of fuel
It reaches.
As shown in figure 8, L-arginine/ultrabranching polyamide nano-particle is in 5% hydrogenperoxide steam generator of mass concentration
Motion state, by can be seen that the motor energy continuous uniform generates NO gases and moving line is curved in figure.
Embodiment 11
It is discharged by the NO of the degradable nano-motor of power source of nitric oxide.
L-arginine/ultrabranching polyamide nano-particle prepared by embodiment 1 is divided into 7 groups, every group of addition 1mL10%
H2O210min, 30min, 1h, 2h, 3h, 6h, 9h are reacted in solution respectively, with manganese dioxide stopped reaction, 12000rpm/min from
The heart takes supernatant, for use.Above-mentioned supernatant nitric oxide kit (nitrate reductase method) is detected to containing for the NO of each sample
Amount.NO release conditions in embodiment 9 are as shown in figure 9, NO is released in the time range of experimental group setting as can be seen from Figure
High-volume continuing to increase, the NO total volumes of 9h are up to 15.9 μm of olL-1And in preceding 1h, the rate of release of NO is very fast, and burst size reaches
The 52% of total volume.
Embodiment 12
Using nitric oxide as the degradation process of the degradable nano-motor of power source.
L-arginine/ultrabranching polyamide nano-particle prepared by embodiment 1 is placed in the peroxide that mass concentration is 10%
Change in hydrogen solution, 1h, 3h, 6h, 18h are incubated at 37 DEG C.12000rpm/min centrifuging and takings lower layer tem observation L-arginine/over-expense
Change the degradation situation of polyamide nano particle, TEM is shown in Figure 10.By can be seen that L-arginine/ultrabranching polyamide is received in Figure 10
Degradation process of the rice corpuscles in 10% hydrogenperoxide steam generator at any time, with the extension in reaction time, L-arginine/over-expense
Changing polyamide nano particle surface gradually becomes coarse, and grain size is gradually reduced.
Claims (9)
1. a kind of L-arginine nano-particle, which is characterized in that passed through by L-arginine and the substance rich in electropositive functional group
The nano-particle that electrostatical binding is self-assembly of.
2. L-arginine nano-particle according to claim 1, which is characterized in that the object rich in electropositive functional group
Matter is ultrabranching polyamide, chitosan or polylysine.
3. a kind of preparation method of L-arginine nano-particle described in claim 1, which is characterized in that by L-arginine and richness
The substance of the functional group containing electropositive is configured to solution respectively, and after the two ultrasonic mixing, centrifuging and taking lower sediment distillation washing is done
It is dry to be made.
4. preparation method according to claim 1, which is characterized in that a concentration of 2.5- of the L-arginine solution
15mg/mL。
5. preparation method according to claim 1, which is characterized in that the substance solution rich in electropositive functional group
A concentration of 0.1-1mg/mL.
6. preparation method according to claim 1, which is characterized in that the L-arginine with positive electricity group is abundant has
Machine object is rich in the substance solution ultrasonic mixing 1-60min of electropositive functional group by 1-10mLL- arginine solutions with 1-10mL.
7. a kind of using nitric oxide as the degradable nano-motor of power source, which is characterized in that the degradable nanometer horse
Up to the nitric oxide generated using L-arginine nano-particle and hydroperoxidation as power source, using hydrogen peroxide as fuel shape
At.
8. the nitric oxide described in a kind of claim 7 is the preparation method of the degradable nano-motor of power source, feature
It is, nitric oxide bubble is released by the redox reaction of L-arginine nano-particle and hydrogen peroxide, pushes nanoparticle
Son movement, becomes nano-motor, with the consumption of L-arginine, L-arginine nano-particle is gradually degraded, and water is finally degraded to
Soluble substance.
9. preparation method according to claim 9, which is characterized in that the mass concentration of the hydrogenperoxide steam generator is 5-
20%.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113402661A (en) * | 2021-05-08 | 2021-09-17 | 南京师范大学 | Zwitterionic polymer-based nitric oxide-driven nano motor and preparation method and application thereof |
| CN113403270A (en) * | 2021-05-08 | 2021-09-17 | 南京师范大学 | Engineered exosome nano motor and preparation method thereof |
| CN113840798A (en) * | 2018-12-05 | 2021-12-24 | 加泰罗尼亚生物工程基础研究所 | Functionalized enzyme driven nanomotor |
| EP3987931A4 (en) * | 2019-12-17 | 2022-10-26 | Universidad de Valladolid | Aqueous solution comprising an inclusion complex, method for obtaining same and use thereof for application in crops to improve their yield |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101138636A (en) * | 2006-09-05 | 2008-03-12 | 中国科学院上海药物研究所 | Gene medicine conveying system and method of preparing the same |
| US20080102128A1 (en) * | 2006-07-28 | 2008-05-01 | Flamel Technologies, Inc. | Modified-release microparticles based on amphiphilic copolymer and on active principles(s) and pharmaceutical formulations comprising them |
| KR20110115660A (en) * | 2010-04-16 | 2011-10-24 | 한국생명공학연구원 | Fluorescence nanoporous silica nanopaticle coated with lipid bilayer and method of preparing the same |
| CN102530853A (en) * | 2012-01-11 | 2012-07-04 | 哈尔滨工业大学 | Preparation method of artificial nanometer pipes and application of utilizing artificial nanometer pipes as nanometer motors |
| CN103011067A (en) * | 2012-12-28 | 2013-04-03 | 哈尔滨工业大学 | Mesoporous silica nanomotor, and preparation method and application thereof |
| CN104395391A (en) * | 2012-03-21 | 2015-03-04 | 恩根尼公司 | Dually derivatized chitosan nanoparticles and methods of making and using the same for gene transfer in vivo |
| CN108051493A (en) * | 2017-11-27 | 2018-05-18 | 山东师范大学 | A kind of preparation method being used for from the polyaminoacid micro-nano motor driven |
-
2018
- 2018-06-11 CN CN201810595299.8A patent/CN108773831B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080102128A1 (en) * | 2006-07-28 | 2008-05-01 | Flamel Technologies, Inc. | Modified-release microparticles based on amphiphilic copolymer and on active principles(s) and pharmaceutical formulations comprising them |
| CN101138636A (en) * | 2006-09-05 | 2008-03-12 | 中国科学院上海药物研究所 | Gene medicine conveying system and method of preparing the same |
| KR20110115660A (en) * | 2010-04-16 | 2011-10-24 | 한국생명공학연구원 | Fluorescence nanoporous silica nanopaticle coated with lipid bilayer and method of preparing the same |
| CN102530853A (en) * | 2012-01-11 | 2012-07-04 | 哈尔滨工业大学 | Preparation method of artificial nanometer pipes and application of utilizing artificial nanometer pipes as nanometer motors |
| CN104395391A (en) * | 2012-03-21 | 2015-03-04 | 恩根尼公司 | Dually derivatized chitosan nanoparticles and methods of making and using the same for gene transfer in vivo |
| CN103011067A (en) * | 2012-12-28 | 2013-04-03 | 哈尔滨工业大学 | Mesoporous silica nanomotor, and preparation method and application thereof |
| CN108051493A (en) * | 2017-11-27 | 2018-05-18 | 山东师范大学 | A kind of preparation method being used for from the polyaminoacid micro-nano motor driven |
Non-Patent Citations (4)
| Title |
|---|
| MARKUS SCHOLL ET AL.: "Dendritic and hyperbranched polyamides.", 《PROGRESS IN POLYMER SCIENCE》 * |
| MIMI WAN ET AL.: "Bio-inspired nitric-oxide-driven nanomotor.", 《NATURE COMMUNICATIONS》 * |
| YU YANG ET AL.: "Advances in self-assembled chitosan nanomaterials for drug delivery.", 《BIOTECHNOLOGY ADVANCES》 * |
| YUE JIN ET AL.: "Synthesis and self-assembly of nonamphiphilic hyperbranched polyoximes.", 《SOFT MATTER》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113840798A (en) * | 2018-12-05 | 2021-12-24 | 加泰罗尼亚生物工程基础研究所 | Functionalized enzyme driven nanomotor |
| EP3987931A4 (en) * | 2019-12-17 | 2022-10-26 | Universidad de Valladolid | Aqueous solution comprising an inclusion complex, method for obtaining same and use thereof for application in crops to improve their yield |
| CN113402661A (en) * | 2021-05-08 | 2021-09-17 | 南京师范大学 | Zwitterionic polymer-based nitric oxide-driven nano motor and preparation method and application thereof |
| CN113403270A (en) * | 2021-05-08 | 2021-09-17 | 南京师范大学 | Engineered exosome nano motor and preparation method thereof |
| CN113402661B (en) * | 2021-05-08 | 2022-07-26 | 南京师范大学 | Zwitterionic polymer-based nitric oxide-driven nano motor and preparation method and application thereof |
| CN113403270B (en) * | 2021-05-08 | 2023-09-22 | 南京师范大学 | Engineering exosome nano motor and preparation method thereof |
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