CN108761072A - 一种磺胺类检测试纸 - Google Patents
一种磺胺类检测试纸 Download PDFInfo
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- CN108761072A CN108761072A CN201810881546.0A CN201810881546A CN108761072A CN 108761072 A CN108761072 A CN 108761072A CN 201810881546 A CN201810881546 A CN 201810881546A CN 108761072 A CN108761072 A CN 108761072A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
Abstract
本发明公开了一种磺胺类检测试纸,包括底板,所述底板上顺次设有样品垫、综合反应垫和吸水垫,所述综合反应垫沿纵向分为免疫反应区和化学反应区,所述免疫反应区通过隔离层与化学反应区分隔,所述免疫反应区包括顺次设置的结合垫和反应膜,所述结合垫上包被有磺胺特异性抗体‑胶体金标记物,所述反应膜上顺次设有磺胺抗生素检测线和羊抗鼠质控线,所述化学反应区包括浸泡检测溶液的载体垫,所述检测溶液包括质量含量25%的硫酸钠、15%的甲醇、2.2%三甲基原甲酸酯、2.2%的冰醋酸。本发明结合化学检测和免疫检测,双重检测有效保证了检测结果的准确性,化学检测时间段、成本低、可视化观察检测结果;免疫检测准确性好,适合家庭使用。
Description
技术领域
本发明属于生物技术应用领域,具体地说是一种磺胺类检测试纸。
背景技术
磺胺类药物是一种广谱抗菌药,在畜牧养殖过程中应用十分广泛,主要用于动物疾病预防和治疗,可以治疗禽伤寒、禽霍乱等多种疾病,但其毒副作用也较为引人注目,能影响泌尿系统和具有一定的致癌性。随着人们生活水平的提高,人们对禽畜的需求量增长,使得养殖场等陆续增多或扩大规模,为了保证经济效益,提高禽畜的成活率,减少病虫威胁,通常过量使用磺胺类药物等抗生素,致使其在动物性食品中、养殖所在地的水体等环境中残留,污染生态环境、威胁人类健康;各种检测方法应运而生,通常实验室检测更受人们关注,但是实验室检测很难快速高效的获得检测结果,不适合人们对快速检测的需求。
发明内容
为了解决上述问题,本发明提供了一种磺胺类检测试纸,包括底板,所述底板上顺次设有样品垫、综合反应垫和吸水垫,所述综合反应垫沿纵向分为免疫反应区和化学反应区,所述免疫反应区通过隔离层与化学反应区分隔,所述免疫反应区包括顺次设置的结合垫和反应膜,所述结合垫上包被有磺胺特异性抗体-胶体金标记物,所述反应膜上顺次设有磺胺抗生素检测线和羊抗鼠质控线,所述化学反应区包括浸泡检测溶液的载体垫,所述检测溶液包括质量含量25%的硫酸钠、15%的甲醇、2.2%三甲基原甲酸酯、2.2%的冰醋酸。
与现有技术相比,本发明的优点是:本发明结合化学检测和免疫检测,双重检测有效保证了检测结果的准确性,化学检测时间段、成本低、可视化观察检测结果;免疫检测准确性好,本发明操作方便、快速,适合家庭使用。
具体实施方式
下面将结合附图对本发明的技术方案作进一步的说明。
如图1所示,一种磺胺类检测试纸,包括底板,所述底板上顺次设有样品垫、综合反应垫和吸水垫,所述综合反应垫沿纵向分为免疫反应区和化学反应区,所述免疫反应区通过隔离层与化学反应区分隔,所述免疫反应区包括顺次设置的结合垫和反应膜,所述结合垫上包被有磺胺特异性抗体-胶体金标记物,所述反应膜上顺次设有磺胺抗生素检测线和羊抗鼠质控线,所述化学反应区包括浸泡检测溶液的载体垫,所述检测溶液包括质量含量25%的硫酸钠、15%的甲醇、2.2%三甲基原甲酸酯、2.2%的冰醋酸。
以上所述仅是本发明的优选实施方式,应当指出:对于本技术领域的普通技术人员来说,在不脱离本发明原理的前提下,还可以做出若干改进和润饰,这些改进和润饰也应视为本发明的保护范围。
Claims (1)
1.一种磺胺类检测试纸,包括底板,其特征在于,所述底板上顺次设有样品垫、综合反应垫和吸水垫,所述综合反应垫沿纵向分为免疫反应区和化学反应区,所述免疫反应区通过隔离层与化学反应区分隔,所述免疫反应区包括顺次设置的结合垫和反应膜,所述结合垫上包被有磺胺特异性抗体-胶体金标记物,所述反应膜上顺次设有磺胺抗生素检测线和羊抗鼠质控线,所述化学反应区包括浸泡检测溶液的载体垫,所述检测溶液包括质量含量25%的硫酸钠、15%的甲醇、2.2%三甲基原甲酸酯、2.2%的冰醋酸。
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Application publication date: 20181106 |