CN108761058A - Paper chip and method for Parallel testing multi-infection disease marker - Google Patents
Paper chip and method for Parallel testing multi-infection disease marker Download PDFInfo
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- CN108761058A CN108761058A CN201810528146.1A CN201810528146A CN108761058A CN 108761058 A CN108761058 A CN 108761058A CN 201810528146 A CN201810528146 A CN 201810528146A CN 108761058 A CN108761058 A CN 108761058A
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
Abstract
The present invention relates to infectious diseases diagnosis marker detection technique fields, and in particular to is used for the paper chip and method of Parallel testing multi-infection disease marker, including upper layer filter paper chip, middle level filter paper chip and lower layer's nitrocellulose filter；Upper layer filter paper chip and lower layer's nitrocellulose filter all include the hydrophobic cofferdam of the multiple hydrophilic areas and rest part that are given off outward by middle part, hydrophilic area includes sample introduction zone and sense channel, and the sense channel of upper layer paper chip is coated with corresponding colloid gold label pathogen antigen and colloid gold label mouse IgG；The sense channel of lower layer's nitrocellulose filter is respectively equipped with detection zone and control zone, and each detection zone is coated with the pathogen antigen of corresponding non-marked respectively, and each control zone packet drapes over one's shoulders sheep anti-mouse igg antibody.Immuno-gold labeling technology is combined with paper chip, realizes Multiple detection and half-quantitative detection, testing result is promptly and accurately by the paper chip and method provided by the present invention for Parallel testing multi-infection disease marker.
Description
Technical field
The present invention relates to the technical fields of infectious diseases and the diagnostic markers analyte detection of other diseases, and in particular to a kind of
Paper chip and method for Parallel testing multi-infection disease marker.
Background technology
In traditional pathogen detection method there are commonly be separately cultured, ELISA (enzyme-linked immunosorbent assay) and PCR
(PCR).Wherein, it is separately cultured and takes and method is complicated, and not all pathogen can be carried out point
From culture；ELISA and PCR high sensitivities, but it is expensive, limit its application in developing country, especially from far-off regions.
These three detection methods are usually required for taking a hour to several hours, and detection time is longer, are not suitable for scene quickly inspection
It tests.
Care diagnostic (point-of-care testing, POCT) is to carry out in sampling location, utilize portable analysis
Instrument and matched reagent quickly obtain a kind of detection mode of testing result, and this detection saves sample in laboratory inspection
Complex process program can quickly obtain inspection result.Immuno-gold labeling technology is one of POCT application technologies, at present this technology with
Lateral chromatography is combined to form colloidal gold strip, colloidal gold strip have it is cheap, quickly, reagent dosage is few, sample size is few,
The advantages that easy to operate, therefore be widely used.Colloidal gold strip testing cost is low, and detection sensitivity and accuracy are still
It is acceptable, it can be used for the extensive screening of disease or the quick diagnosis of part disease.
Existing colloidal gold strip detection method has as a drawback that (1) is merely able to carry out qualitative detection, cannot carry out
Quantitative detection.(2) single disease can only be detected, Multiple detection is can not achieve.(3) sensitivity is not so good as ELISA.Due to antigen-antibody
Incubation time is short or antibody can influence its sensitivity with cellulose membrane non-specific interaction.
Micro-fluidic chip (microfluidics) is also known as Microfluid based Lab on a chip or chip lab (lab-on-a-
Chip, LOC), it is a kind of new technology to grow up on the basis of Capillary Electrophoresis the 1990s, passes through micro-processing technology
The element of the different function such as microchannel, microreactor, microelectrode, micro- detector is integrated, is had a biology or change
Learn the ability on laboratory micro to one piece only several square centimeters of thin slices.Microflow control technique is because sample volume needed for it is small, inspection
Survey that efficient, use cost is low and be easy to other technologies integration of equipments, there is good compatibility, be expected to realize portable inspection
The features such as surveying device, has attracted the concern of numerous researchers.In recent years, microfluidic chip technology is oozed to biomedical sector rapidly
Thoroughly, it is shown that wide application prospect, more and more signs show that this technology has become medical research of new generation and medicine inspection
Survey extremely important platform.
Micro-fluidic refill sheet devices are mainly made of materials such as filter paper or cellulose membranes, are simplest in micro-fluidic chip
It is a kind of.Micro-fluidic paper chip can complete the detection program of other devices complexity as long as seldom sample and reagent；White
Paper chip makes many detections carried out using color change become more convenient；Paper chip burning after use can destroy,
Processing is simple, does not generate hazard residue.In recent years, medically, paper chip is in Protein Detection, blood separation, cell culture etc.
Aspect is quickly grown.
Invention content
In order to solve the above technical problems, The present invention provides one kind being used for Parallel testing multi-infection disease marker
Paper chip and method.
In order to reach above-mentioned technique effect, the present invention includes following technical scheme：One kind being used for Parallel testing multi-infection
The paper chip of property disease marker, including upper layer filter paper chip, middle level filter paper chip and lower layer's nitrocellulose filter；The upper layer
Filter paper chip and lower layer's nitrocellulose filter all respectively include the multiple hydrophilic areas given off outward by middle part and rest part
Hydrophobic cofferdam, the hydrophilic area includes sample introduction zone and sense channel, and the sample introduction zone of each hydrophilic area converges at corresponding filter paper chip
Middle part, the sense channel of each hydrophilic area of the upper layer paper chip is coated with corresponding colloid gold label pathogen antigen and glue
Body gold marks mouse IgG；The end of the liquid flow direction of the sense channel of the upper layer filter paper chip is corresponded on the middle level filter paper chip
End position is equipped with logical sample mouth；The sense channel of each hydrophilic area of lower layer's nitrocellulose filter is by along liquid flow direction point
Not She You detection zone and control zone, each detection zone is coated with the pathogen antigen of corresponding non-marked respectively, and each control zone packet drapes over one's shoulders sheep
Dynamics.
Preferably, the upper layer filter paper chip and lower layer's nitrocellulose filter are all respectively equipped with four hydrophilic areas, Mei Geqin
The sense channel in pool detects an index.
Preferably, the upper layer filter paper chip gives off four sense channels, Mei Gejian around by the sample introduction zone at middle part
Survey channel be coated with respectively colloid gold label pathogen Staphylococal Protein A+colloid gold label mouse IgG, colloid gold label pathogen B antigens+
Colloid gold label mouse IgG, colloid gold label pathogen C antigens+colloid gold label mouse IgG and colloid gold label pathogen D are anti-
Original+colloid gold label mouse IgG；Detection zone and four control zones there are four being corresponded in lower layer's nitrocellulose filter, described four
A detection zone is respectively detection zone A, detection zone B, detection zone C and detection zone D, and correspondence is coated with pathogen A to each detection zone respectively
Antigen, pathogen B antigens, pathogen C antigens and pathogen D antigens；Four control zones are located at four sense channels
On, each control zone is coated with sheep anti-mouse igg antibody.
Preferably, the distance between the detection zone in each sense channel of lower layer's nitrocellulose filter and control zone
For 1-15mm.
Preferably, the paper chip is formed by wax spray printing and making, between each layer by printing glue and lamination assembling
At.
As a further improvement on the present invention, further include upper layer gasket, haemocyte filter pad, water absorption pad and underlying shims,
The upper layer gasket is located at the top of the upper layer filter paper chip, and sample application zone, the haemocyte are equipped in the middle part of the upper layer gasket
Filter pad is located at the sample introduction section of the sample application zone and the middle part of the upper layer filter paper chip；The underlying shims be located at it is described under
Layer nitrocellulose filter lower part, the water absorption pad are located in the middle part of lower layer's nitrocellulose filter and underlying shims middle part
Between.
Preferably, the upper layer gasket and underlying shims are all acrylic gasket.
Preferably, the thickness of the haemocyte filter pad is 200-1800um.
On the other hand, above-mentioned paper chip Parallel testing multi-infection disease marker is used the present invention also provides a kind of
Method, include the following steps：
(1) sample to be detected is flowed into sense channel by the sample introduction zone in the middle part of the filter paper chip of upper layer；
(2) antigen of the pathogenic autoantibody association colloid gold label in sample continues the logical sample mouth through middle level filter paper chip downwards
Flow through the detection zone of lower layer's nitrocellulose filter；
(3) if there is detection target pathogen antibody, capture is combined colloidal gold by the non-labeled antigen of detection zone
Target antibody causes colloidal gold aggregation, generates measurable color change；The mouse IgG of colloid gold label will not be captured, and continue
It moves forward, is captured by sheep anti-mouse igg when reaching control zone, generate measurable color change, sentenced by colour switching
Break and the presence or absence of pathogen；
(4) by mobile phone photograph app photographic analysis or it is back to computer for analysis, quantitative color variable quantity, and in advance really
Fixed standard curve is compared, the quantity of quantitative pathogen.
Optionally, the sample includes but not limited to whole blood, blood plasma, serum, dried blood spot, tissue fluid, saliva, tear, sweat
Liquid.
Using above-mentioned technical proposal, including following advantageous effect：It is provided by the present invention to be used for Parallel testing multi-infection
Property disease marker paper chip and method, immuno-gold labeling technology is combined with paper chip, can detect whole blood sample, a drop
Multiple infectious disease is surveyed in blood examination, realizes Multiple detection and half-quantitative detection, and testing result is promptly and accurately, at low cost and can realize greatly
Scale mass production.
Description of the drawings
Fig. 1 is the paper chip for Parallel testing multi-infection disease marker provided in the embodiment of the present invention 1
Structural schematic diagram；
Fig. 2 is in the embodiment of the present invention 1 for the upper layer in the paper chip of Parallel testing multi-infection disease marker
The structural schematic diagram of filter paper chip；
Fig. 3 is in the embodiment of the present invention 1 for the lower layer in the paper chip of Parallel testing multi-infection disease marker
The structural schematic diagram of nitrocellulose filter；
Fig. 4 is the schematic diagram of upper layer filter paper chip provided in the embodiment of the present invention 2；
Fig. 5 is lower layer's nitrocellulose membrane structure diagram provided in the embodiment of the present invention 2；
Fig. 6 is the schematic diagram of upper layer filter paper chip provided in the embodiment of the present invention 3；
Fig. 7 is lower layer's nitrocellulose membrane structure diagram provided in the embodiment of the present invention 3；
The standard curve for the hepatitis B surface antibody that Fig. 8 is provided by the embodiment of the present invention 2；
The standard curve for the syphilis antibody that Fig. 9 is provided by the embodiment of the present invention 2；
The standard curve for the c-hepatitis antibody that Figure 10 is provided by the embodiment of the present invention 2.
In figure,
1, upper layer gasket；1.1, sample application zone；2, haemocyte filter pad；3, upper layer filter paper chip；3.1, colloid gold label disease
Substance Staphylococal Protein A+colloid gold label mouse IgG；3.2, colloid gold label pathogen B antigens+colloid gold label mouse IgG；3.3, colloid
Gold label pathogen C antigens+colloid gold label mouse IgG；3.4, colloid gold label pathogen D antigens+colloid gold label mouse IgG；
4, middle level filter paper chip；4.1, lead to sample mouth；5, lower layer's nitrocellulose filter；5.1, detection zone A；5.2, detection zone B；5.3, it detects
Area C；5.4, detection zone D；5.5, control zone；6, water absorption pad；7, underlying shims；100, sample introduction zone；200, sense channel.
Specific implementation mode
To make the object, technical solutions and advantages of the present invention clearer, below in conjunction with attached in the embodiment of the present invention
Figure, technical scheme in the embodiment of the invention is clearly and completely described, it is clear that described embodiment is the present invention
A part of the embodiment, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art are not having
The every other embodiment obtained under the premise of creative work is made, shall fall within the protection scope of the present invention.
In the present invention, term "upper", "lower", "left", "right", "front", "rear", "top", "bottom", "inner", "outside",
" in ", "vertical", "horizontal", " transverse direction ", the orientation or positional relationship of the instructions such as " longitudinal direction " be orientation based on ... shown in the drawings or
Position relationship.These terms are not intended to limit indicated dress primarily to preferably describe the present invention and embodiment
It sets, element or component must have particular orientation, or be constructed and operated with particular orientation.
Also, above-mentioned part term is other than it can be used to indicate that orientation or positional relationship, it is also possible to for indicating it
His meaning, such as term "upper" also are likely used for indicating certain relations of dependence or connection relation in some cases.For ability
For the those of ordinary skill of domain, the concrete meaning of these terms in the present invention can be understood as the case may be.
In addition, term " installation ", " setting ", " being equipped with ", " connection ", " connected " " socket " shall be understood in a broad sense.For example, can
To be to be fixedly connected, it is detachably connected or monolithic construction；Can be mechanical connection, or electrical connection；It can be directly connected,
Either indirectly connected through an intermediary, or it is two connections internal between device, element or component.For
For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific conditions.
Unless otherwise indicated, the meaning of " multiple " is two or more.
It is described in further detail below by specific embodiment and in conjunction with attached drawing to the present invention.
Embodiment 1：
A kind of paper chip for Parallel testing multi-infection disease marker is present embodiments provided, refering to fig. 1, packet
Include upper layer filter paper chip 3, middle level filter paper chip 4 and lower layer's nitrocellulose filter 5；The upper layer filter paper chip 3 and lower layer's nitric acid
Cellulose membrane 5 all respectively includes the hydrophobic cofferdam of the multiple hydrophilic areas and rest part that are given off outward by middle part, the parent
Pool includes sample introduction zone 100 and sense channel 200, and the sample introduction zone of each hydrophilic area converges at the middle part of corresponding filter paper chip, described
The sense channel 200 of each hydrophilic area of upper layer filter paper chip 3 is coated with corresponding colloid gold label pathogen antigen and colloidal gold
Mark mouse IgG；The end of the liquid flow direction of the sense channel of the upper layer filter paper chip 3 is corresponded on the middle level filter paper chip 4
Position is equipped with logical sample mouth 4.1；The sense channel of each hydrophilic area of lower layer's nitrocellulose filter 5 is by along liquid flow direction
It is respectively equipped with detection zone and control zone 5.5, each detection zone is coated with the pathogen antigen of corresponding non-marked, each control zone packet respectively
Drape over one's shoulders sheep anti-mouse igg antibody.
Port number regards actual needs, can for 2-16 between any number of channel, in the present embodiment, in upper layer filter
Paper chip 3 and lower layer's nitrocellulose filter 5 are all respectively equipped with four hydrophilic areas, and the sense channel of each hydrophilic area detects a finger
Mark.Paper chip is prepared using the method that wax spray prints, it is also possible to prepared by other methods in the present embodiment.It is hydrophilic logical by designing
Road is printed by wax spray printer, so that wax is melted infiltration using baking process and is formed hydrophobic cofferdam, to control the stream of liquid
Dynamic direction.Can also use ultraviolet photolithographic, wax printing, corona treatment, inkjet printing, ink-jet carve solely, drawing, silk-screen printing,
The technologies such as wax melting is impregnated with, wax melting seal, flexo, laser treatment are processed.It is assembled between each layer by printing glue and lamination
It forms.The preparation method of three-dimensional paper chip is the common knowledge of those skilled in the art, is not repeated herein.
Referring to Fig. 2 and 3, the upper layer filter paper chip 3 gives off four sense channels around by the sample introduction zone 100 at middle part
200, each sense channel is coated with colloid gold label pathogen Staphylococal Protein A+colloid gold label mouse IgG (3.1), colloidal gold mark respectively
Remember pathogen B antigens+colloid gold label mouse IgG (3.2), colloid gold label pathogen C antigens+colloid gold label mouse IgG (3.3)
And colloid gold label pathogen D antigens+colloid gold label mouse IgG (3.4)；It is corresponding in lower layer's nitrocellulose filter 5
Four detection zones and four control zones, four detection zones are respectively detection zone A (5.1), detection zone B (5.2), detection zone C
(5.3) it corresponds to is coated with pathogen Staphylococal Protein A, pathogen B antigens, pathogen C antigens respectively with detection zone D (5.4), each detection zone
With pathogen D antigens；Four control zones 5.5 are located on four sense channels, and each control zone is coated with sheep anti-mouse igg
Antibody.
The distance between detection zone and control zone in each sense channel of lower layer's nitrocellulose filter 5 are 1-
15mm.The paper chip is formed by wax spray printing and making, is assembled by printing glue and lamination between each layer.
Further include upper layer gasket 1, haemocyte filter pad 2, water absorption pad 6 and underlying shims 7, the upper layer in the present embodiment
Gasket 1 is located at the top of the upper layer filter paper chip 3, and 1 middle part of the upper layer gasket is equipped with sample application zone 1.1, the haemocyte mistake
Filter bed 2 is located between the sample application zone 1.1 and the sample introduction zone 100 at the middle part of the upper layer filter paper chip 3；The underlying shims 7
In 5 lower part of lower layer's nitrocellulose filter, the water absorption pad 6 is located in the middle part of lower layer's nitrocellulose filter 5 and under described
Between 7 middle part of layer gasket.Wherein, the upper layer gasket 1 and underlying shims 7 are all acrylic gasket.The haemocyte filter pad
Thickness is 200-1800um.
Paper chip provided in the present embodiment, it is preferred to use wax spray printing and making is filtered at three-dimensional paper chip by upper layer
Paper chip, middle level filter paper chip and lower layer's nitrocellulose filter form the flow direction design of inflection, reduce overall chip size.It is logical
Immuno-gold labeling technology detection multiple infectious disease is crossed, realizes high-throughput detection.By printing glue and lamination assembling chip, micelle
It is evenly distributed and does not need extra padding cellulose powder, be suitble to large-scale batch production.Paper chip and colloidal gold strip knot
It closes and uses, be more suitable for Multiple detection；It prepares simple；It is few to be coated with object dosage, it is at low cost.Detectable communicable disease, it can also be used to
The detections such as Other diseases marker.
Using the method for above-mentioned paper chip Parallel testing multi-infection disease marker, include the following steps：It will be to be checked
Whole blood about 20ul is added by the sample application zone of upper layer gasket in the sample of survey, and blood removes haemocyte by haemocyte filter pad, then
It passes downwardly through the sample introduction zone in the middle part of the filter paper chip of upper layer and is flowed into sense channel；
The antigen of pathogenic autoantibody association colloid gold label in sample continues the logical sample mouth stream through middle level filter paper chip downwards
Detection zone through lower layer's nitrocellulose filter；
If there is detection target pathogen antibody, capture is combined the target of colloidal gold by the non-labeled antigen of detection zone
Antibody causes colloidal gold aggregation, generates measurable color change；The mouse IgG of colloid gold label will not be captured, and continue forward
It is mobile, it is captured by sheep anti-mouse igg when reaching control zone, generates measurable color change, judged by colour switching
The presence or absence of pathogen；
By mobile phone photograph app photographic analysis or it is back to computer for analysis, quantitative color variable quantity is and predetermined
Standard curve is compared, the quantity of quantitative pathogen.
It should be noted that the sample includes but not limited to whole blood, and blood plasma, serum, dried blood spot, tissue fluid, saliva, tear
Liquid, sweat.
Embodiment 2：
On the basis of embodiment 1, present embodiments provide a kind of using paper chip Parallel testing hepatitis B table in embodiment 1
The method of face antigen, hepatitis B surface antibody, c-hepatitis antibody and syphilis antibody, includes the following steps：
The acrylic for being 1mm with thickness processes upper layer gasket and underlying shims respectively.Upper layer filter paper chip and middle level filter paper
Chip selects Whatman filter paper.It is the nitrocellulose paper of 0.45um that lower layer, which selects aperture,.Select the filtering of 200um thickness haemocytes
Pad and 500um thickness water absorption pads.
Hydrophobic cofferdam is printed with wax spray printer, prepares patterning chip.In chip upper layer filter paper chip different zones
(different sense channels) is coated with HbsAb antigens (100ug/mL), HbsAg antigens (200ug/mL), the HCV of colloid gold label respectively
It is mixed simultaneously in (hepatitis C, 200ug/mL) antigen and TP (microspironema pallidum, 200ug/mL) antigen, the above antigen or antibody
The mouse IgG (100ug/mL) of colloid gold label, as Quality Control internal reference, as shown in Figure 4.
The pathogen antigen of non-marked is coated in each detection zone of lower layer's nitrocellulose filter, sheep anti-mouse igg antibody coating
In four control zones of lower layer's nitrocellulose filter.Layer nitrocellulose filter corresponding region (different sense channels) point under the die
It Bao Bei not HBsAb secondary antibodies (100ug/mL), HBsAg antigens (200ug/mL), HCV antigens (hepatitis C, 200ug/mL)
With TP antigens (microspironema pallidum, 200ug/mL), as shown in Figure 5.
The patterning print collodion silk net of 150 mesh is chosen, tension 15N/cm, (main component is that the paper of epoxy resin is viscous by glue
Glue) be printed on the front and back of upper layer filter paper chip, lower layer's nitrocellulose filter, silk-screen patterns respectively with upper layer filter paper core
Piece is consistent with lower layer's nitrocellulose filter, and away from being 1.5mm, scraper plate connects net with collodion silk net is printed between print collodion silk net and filter paper when printing
Angle is 45 ° when touching, and print speed printing speed is 0.4m/s, and it is 4N that paper, which bears pressure, and haemocyte filter pad and upper layer gasket are stacked
Again with upper layer filter paper die bonding, then upper layer filter paper chip and middle level filter paper die bonding, are bonded to entire paper chip successively
Assembly is completed.
When work, whole blood about 20ul is added from the sample application zone of upper layer gasket, blood filters off blood by haemocyte filter pad
Cell, when down through upper layer filter paper chip, the antigen of pathogenic autoantibody association colloid gold in sample label, continue to
Under, when flowing through lower layer's nitrocellulose filter detection zone, if there is detection target pathogen antibody, the non-labeled antigen of detection zone
The target antibody that capture is combined to colloidal gold, causes colloidal gold aggregation, generates measurable color change.Colloid gold label
Mouse IgG will not be captured, and continue to move along, and captured by sheep anti-mouse igg when reaching control zone, generate measurable color
Variation.
By color change may determine that pathogen with/without；
It is back to computer for analysis by mobile phone photograph, gray analysis is carried out to image with imageJ, compared with standard curve,
Determine the quantity of pathogen.When detecting pathogen antigen, colloid gold label antigen is substituted for colloid gold label first antibody, non-
Labelled antigen replaces with non-marked secondary antibody.
Embodiment 3：
On the basis of embodiment 1, present embodiments provides and screening lung cancer is assisted using the paper chip that embodiment 1 is provided
The method for detecting lung cancer marker.
This method can be used for detecting Lung Cancer Associated Antigen：It is Carcinoembryonic Antigen CEA, neuronspecific enolase NSE, thin
Born of the same parents' Keratin 19 soluble antigen Cyfra21-1, squamous cell carcinoma antigen SCCA.
Hydrophobic cofferdam is printed with wax spray printer, prepares patterning chip.On chip upper layer, filter paper core different zones are (no
Same sense channel) it is coated with monoclonal antibody CEA (200ug/mL), NSE (500ug/mL), the Cyfra21- of colloid gold label respectively
1 (300ug/mL) and SCCA (300ug/mL).Mouse IgG (the 100ug/ that colloid mixture gold marks simultaneously in the above antigen or antibody
ML), as Quality Control internal reference, as shown in Figure 6.
Refering to Fig. 7, the pathogen antigen of non-marked is coated in the detection zone of lower layer's nitrocellulose filter, and sheep anti-mouse igg is anti-
Body is coated in four control zones of lower layer's nitrocellulose filter.Layer nitrocellulose filter corresponding region (difference detection under the die
Channel) respectively coating monoclonal secondary antibody CEA (300ug/mL), NSE (300ug/mL), Cyfra21-1 (300ug/mL) and
SCCA(300ug/mL).Upper layer gasket and underlying shims are processed with 1mm acrylics.Select 200um thickness haemocyte filter pads and
500um thickness water absorption pads.Gluing method, chip assembling are printed with interpretation of result with example 2.
The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention, for the skill of this field
For art personnel, the invention may be variously modified and varied.All within the spirits and principles of the present invention, any made by repair
Change, equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.
Claims (10)
1. a kind of paper chip for Parallel testing multi-infection disease marker, which is characterized in that including upper layer filter paper core
Piece (3), middle level filter paper chip (4) and lower layer's nitrocellulose filter (5)；The upper layer filter paper chip (3) and lower layer's cellulose nitrate
Plain film (5) all respectively includes the hydrophobic cofferdam of the multiple hydrophilic areas and rest part that are given off outward by middle part, described hydrophilic
Area includes sample introduction zone (100) and sense channel (200), and the sample introduction zone (100) of each hydrophilic area converges in corresponding filter paper chip
The sense channel in portion, each hydrophilic area of the upper layer paper chip (3) is coated with corresponding colloid gold label pathogen antigen and glue
Body gold marks mouse IgG；The liquid flow of the sense channel of the upper layer filter paper chip (3) is corresponded on the middle level filter paper chip (4)
To terminal position be equipped with logical sample mouth (4.1)；The sense channel of each hydrophilic area of lower layer's nitrocellulose filter (5) by along
Liquid flow direction is respectively equipped with detection zone and control zone, and each detection zone is coated with the pathogen antigen of corresponding non-marked respectively,
Each control zone packet drapes over one's shoulders sheep anti-mouse igg antibody.
2. the paper chip according to claim 1 for Parallel testing multi-infection disease marker, which is characterized in that
The upper layer filter paper chip (3) and lower layer's nitrocellulose filter (5) are all respectively equipped with four hydrophilic areas, the detection of each hydrophilic area
One index of Air conduct measurement.
3. the paper chip according to claim 2 for Parallel testing multi-infection disease marker, which is characterized in that
The upper layer filter paper chip (3) gives off four sense channels around by the sample introduction zone at middle part, and each sense channel wraps respectively
There are colloid gold label pathogen Staphylococal Protein A+colloid gold label mouse IgG (3.1), colloid gold label pathogen B antigens+colloidal gold mark
Remember mouse IgG (3.2), colloid gold label pathogen C antigens+colloid gold label mouse IgG (3.3) and colloid gold label pathogen D
Antigen+colloid gold label mouse IgG (3.4)；It is corresponding in lower layer's nitrocellulose filter (5) that there are four detection zones and four controls
Area, four detection zones are respectively detection zone A (5.1), detection zone B (5.2), detection zone C (5.3) and detection zone D (5.4),
Correspondence is coated with pathogen Staphylococal Protein A, pathogen B antigens, pathogen C antigens and pathogen D antigens to each detection zone respectively；Described four
A control zone (5.5) is located on four sense channels, and each control zone is coated with sheep anti-mouse igg antibody.
4. the paper chip according to claim 1 for Parallel testing multi-infection disease marker, which is characterized in that
The distance between detection zone and control zone in each sense channel of lower layer's nitrocellulose filter (5) are 1-15mm.
5. the paper chip according to claim 1 for Parallel testing multi-infection disease marker, which is characterized in that
The paper chip is formed by wax spray printing and making, is assembled by printing glue and lamination between each layer.
6. the refill for Parallel testing multi-infection disease marker according to claim 1-5 any one
Piece, which is characterized in that further include upper layer gasket (1), haemocyte filter pad (2), water absorption pad (6) and underlying shims (7), it is described on
Layer gasket (1) is located at the top of the upper layer filter paper chip (3), is equipped with sample application zone (1.1) in the middle part of the upper layer gasket (1), institute
State the sample introduction zone (100) that haemocyte filter pad (2) is located at the sample application zone (1.1) and the middle part of the upper layer filter paper chip (3)
Between；The underlying shims (7) are located at lower layer's nitrocellulose filter (5) lower part, and the water absorption pad (6) is located at lower layer's nitre
Between in the middle part of acid cellulose film (5) and in the middle part of the underlying shims (7).
7. the paper chip according to claim 6 for Parallel testing multi-infection disease marker, which is characterized in that
The upper layer gasket (1) and underlying shims (7) are all acrylic gasket.
8. the paper chip according to claim 6 for Parallel testing multi-infection disease marker, which is characterized in that
The thickness of the haemocyte filter pad (2) is 200-1800um.
9. according to the method for paper chip Parallel testing multi-infection disease marker described in claim 1-8 any one,
It is characterised in that it includes following steps：
(1) sample to be detected is flowed into sense channel by the sample introduction zone in the middle part of the filter paper chip of upper layer；
(2) the logical sample mouth through middle level filter paper chip flows through downwards for the antigen continuation of the pathogenic autoantibody association colloid gold label in sample
The detection zone of lower layer's nitrocellulose filter；
(3) if there is detection target pathogen antibody, capture is combined the target of colloidal gold by the non-labeled antigen of detection zone
Antibody causes colloidal gold aggregation, generates measurable color change；The mouse IgG of colloid gold label will not be captured, and continue forward
It is mobile, it is captured by sheep anti-mouse igg when reaching control zone, generates measurable color change, judged by colour switching
The presence or absence of pathogen；
(4) by mobile phone photograph app photographic analysis or it is back to computer for analysis, quantitative color variable quantity is and predetermined
Standard curve is compared, the quantity of quantitative pathogen.
10. according to the method described in claim 9, it is characterized in that, the sample includes but not limited to whole blood, blood plasma, serum,
Dried blood spot, tissue fluid, saliva, tear, sweat.
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