CN108753643B - Vibrio H11 and application thereof - Google Patents
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Abstract
The invention relates to vibrio H11 and application thereof, wherein the vibrio H11 is preserved in China center for type culture Collection with the preservation number of CCTCC NO: m2018172. The bacterial strain capable of efficiently hydrolyzing the enteromorpha polysaccharide is screened, the reducing sugar obtained by hydrolysis of the bacterial strain can be utilized by an energy bacterial strain to generate a biological energy substance, and compared with the existing single bacterial strain for hydrolyzing the enteromorpha polysaccharide, the vibrio H11 in the invention has the strongest hydrolysis capability. The invention realizes high-value utilization of enteromorpha through the combination of the hydrolysis of vibrio and the anaerobic fermentation of clostridium.
Description
Technical Field
The invention belongs to the field of microbial biotechnology and resource biotechnology, and particularly relates to vibrio H11 and application thereof.
Background
Enteromorpha (Enteromorpha) is a large green alga, and belongs to the plant of the Chlorophyta (Chlorophyta) order Ulvales (Ulvalles) family Ulvaceae (Ulvaceae). In recent years, due to global warming and seawater eutrophication, enteromorpha can grow rapidly, and great influence is brought to the marine ecological environment. In the early stage, the enteromorpha is buried and burned, so that the surrounding water and soil are seriously polluted, and the waste of biomass resources is caused. How to effectively utilize the explosive resource of enteromorpha has become a research hotspot for improving the marine ecological environment and comprehensively utilizing the seaweed.
Enteromorpha as a large green alga is a potential renewable biofuel source and can be used for preparing bio-oil, methane or ethanol and the like. However, due to the complex and unclear components of the enteromorpha polysaccharide, researchers have less research on high-value utilization of enteromorpha and have little application of the enteromorpha and the enteromorpha polysaccharide in the aspects of active oligosaccharide or biological energy. At present, the enteromorpha is mainly treated by a hydrothermal method, a rapid pyrolysis method and a fermentation method. But the fuel oil obtained by a hydrothermal method and a fast pyrolysis method is difficult to be directly utilized; therefore, the microbial enzyme method is favored by a large number of researchers. At present, the main carbon sources for producing alcohols and acids by microorganisms are monosaccharides such as glucose, xylose and fructose, and the cost is too high. However, the enteromorpha prolifera which is an explosive resource can be hydrolyzed into fermentable reducing sugar which is used as a novel biological energy source in raw materials.
Disclosure of Invention
The invention aims to provide vibrio H11 and application thereof, and aims to solve the problems that researches on high-value utilization of enteromorpha by researchers are few due to complex and unclear enteromorpha polysaccharide components at present, so that the scheme for researching the enteromorpha utilization is single, the method is mainly a physical and chemical method at present, and the method has great limitation and the like.
In order to solve the problems, the invention provides Vibrio H11(Vibrio sp.h11) which is preserved in the China center for type culture Collection in 31/3/2018, wherein the preservation numbers are as follows: CCTCC NO: m2018172, depository address: wuhan university collection, eight-way 299 in Wuchang area, Wuhan city, Hubei province. The strain can grow in a culture medium with enteromorpha polysaccharide as a unique carbon source, and is a gram-negative bacterium; the physiological and biochemical characteristics are positive VP, positive MR and negative gelatin liquefaction. Can grow and hydrolyze enteromorpha green tide biomass serving as a unique carbon source and energy into clostridium fermentable reducing sugar for anaerobic fermentation to generate biological hydrogen, biological organic solvent and the like.
The culture method of the vibrio H11 comprises the following steps: inoculating the Vibrio H11 strainThe enteromorpha polysaccharide is used as the only carbon source for growth, and the composition of the culture medium is (1L) (NH)4)2SO4，1.0g；Na2HPO4，0.8g；KH2PO4， 0.2g；MgSO4·7H2O，0.2g；CaCl2·2H2O，0.1g；FeCl3·6H2O，5mg； (NH4)6Mo7O24·4H2O，1mg；ddH2O1L; 20g of enteromorpha polysaccharide; tryptone, 5g/L, was cultured at 30 ℃ and pH 8.71 for 12 h. The medium was first sterilized at 121 ℃ for 20 min.
The application of vibrio H11 can be used for hydrolyzing green tide biomass. The method is mainly used for hydrolyzing the enteromorpha into the reducing sugar which can be fermented by the clostridium. The vibrio H11 is used for hydrolyzing enteromorpha polysaccharide in enteromorpha into reducing sugar and then converting the reducing sugar into a biological energy substance through anaerobic fermentation of clostridium. The application method mainly comprises the following steps: adding concentrated crude enzyme solution containing Vibrio H11 into anaerobic fermentation culture medium containing Enteromorpha powder or Enteromorpha polysaccharide, and hydrolyzing in a shaker at 40 deg.C and 200rpm for 48H. Then inoculating clostridium for anaerobic fermentation. The Clostridium may be Clostridium acetobutylicum WA strain.
Enteromorpha polysaccharide is heteropolysaccharide with a complex structure, only the main components of the enteromorpha polysaccharide are known, and the structure of the enteromorpha polysaccharide is not clearly reported at present. For the hydrolysis of the enteromorpha polysaccharide, the mixed flora has better hydrolysis capacity, but the flora composition is easy to change and is difficult to store. The Vibrio sp.H11 strain is used as a single strain, and the enteromorpha polysaccharide hydrolyzing capability of the strain is strongest. For comprehensive treatment and high-value utilization of green tide enteromorpha biomass, the current physical and chemical method has high cost requirement, and the generated waste liquid is easy to pollute the environment. According to the invention, the enteromorpha is finally converted into the biological energy substance by a microbial method, so that the cost is low and the environment is not polluted. At present, the enteromorpha is converted into a biological energy substance by utilizing a microbial fermentation method for the first time.
Compared with the prior art, the bacterial strain capable of efficiently hydrolyzing the enteromorpha polysaccharide is screened, the reducing sugar obtained by hydrolysis of the bacterial strain can be utilized by the energy bacterial strain to generate a biological energy substance, and compared with the existing single bacterial strain for hydrolyzing the enteromorpha polysaccharide, the vibrio H11 in the invention has the strongest hydrolysis capability. The invention realizes high-value utilization of enteromorpha through the combination of the hydrolysis of vibrio and the anaerobic fermentation of clostridium.
Drawings
Fig. 1 is a phylogenetic tree of Vibrio sp.h 11;
fig. 2 is an electron micrograph of Vibrio sp.h 11;
fig. 3 is a lugol's iodine staining pattern of Vibrio sp.h 11;
FIG. 4 is a graph showing the growth curve of Vibrio sp.H11 and the reducing sugar content in the fermentation supernatant;
FIG. 5 is a graph of the enzymatic activity of crude Vibrio sp.H11 enzyme;
fig. 6 is the amount of bio-hydrogen produced by anaerobic fermentation of a c.acetobutylicum WA strain using different fermentable reducing sugars;
fig. 7 is the amount of biosolvent produced by the c.acetobutylicum WA strain with different levels of fermentable reducing sugars of enteromorpha polysaccharide;
fig. 8 is the amount of biosolvent produced by the c.acetobutylicum WA strain with different levels of fermentable reducing sugar of enteromorpha powder.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in further detail with reference to the accompanying drawings.
Example 1
(1) Screening culture of Vibrio sp.H11
Obtaining a green alga sample in the offshore sea area of Hainan at the early stage, rinsing the sample by using sterile physiological saline, grinding the sample into homogenate by using a sterile mortar, standing, slightly sucking 1mL of supernatant by using a liquid transfer gun, adding the supernatant into an enteromorpha polysaccharide inorganic salt culture medium, and culturing for one day at 25 ℃ and 150 rpm; and transferring 1mL of fermentation broth to a new enteromorpha polysaccharide inorganic salt culture medium for continuous culture, and repeating the operation for 3 times. The obtained fermentation liquor is treated according to the method 10-3、10-4、 10-5、10-6、10-7Performing gradient dilution, and then coating the enteromorpha polysaccharide plate with the enteromorpha polysaccharide to perform inverted culture for one day at 25 ℃. Observing morphology, picking single colony and streaking for multiple times to obtain pure culture. And then preliminarily determining whether the strain has enteromorpha polysaccharide hydrolysis capability or not through the content of reducing sugar in the enteromorpha polysaccharide inorganic salt culture medium fermentation liquor (DNS method). Finally obtaining the vibrio H11 with the highest enzyme activity.
Wherein the enteromorpha polysaccharide inorganic salt culture medium mainly comprises 1L (NH)4)2SO4，1.0g； Na2HPO4，0.8g；KH2PO4，0.2g；MgSO4·7H2O，0.2g；CaCl2·2H2O，0.1g； FeCl3·6H2O，5mg；(NH4)6Mo7O24·4H2O, 1L of enteromorpha polysaccharide leaching liquor.
(2) Identification of Vibrio sp.H11
Vibrio sp.h11 is a gram-negative strain; the physiological and biochemical characteristics are positive VP, positive MR and negative gelatin liquefaction; the following substances can be used as carbon sources: d-fructose, D-glucose, D-xylose, D-galactose, sucrose, mannitol, citric acid and starch; the results are shown in Table 1.
TABLE 1 physio-biochemical characteristics of Vibrio sp.H11
A colony PCR method is adopted, and a single colony is directly taken as a template for PCR. The primers for amplifying the 16S rDNA by using PCR are a pair of universal primers, 27F: 5'-AGAGTTTGATCCTGGCTCAG-3', 1492R: 5'-GGTTACCTTGTTACGACT-3' are provided. The PCR reaction conditions are as follows: 3min at 94 ℃; 30 cycles of 95 ℃ for 30s, 55 ℃ for 30s, and 72 ℃ for 90 s; 10min at 72 ℃. After purifying the PCR amplification product, sending a sequencing result to perform Blast analysis on the sequencing result and a sequence on Genbank, wherein the sequence length is 1385bp, and the invention has great homology with Vibrio coralliilyticus (Vibrio coralliilyticus); the 16S rDNA sequence is shown in SEQ ID NO. 1. The sequence is compared with the sequence in Genbank, similar strains are selected, a phylogenetic tree is constructed by MEGA7 software, and the constructed dendrogram is shown in figure 1. Meanwhile, it was found by electron microscopy of Vibrio H11 that it was rod-shaped and had flagella as shown in FIG. 2.
Vibrio H11 was cultured on an inorganic salt medium plate containing 2% Enteromorpha polysaccharide for 12 hours, and then stained with Lugol's iodine solution to have a clear transparent circle, as shown in FIG. 3.
The vibrio H11 grows by taking enteromorpha polysaccharide as a unique carbon source, generates related glycoside hydrolase, and breaks different types of glycosidic bonds in the enteromorpha polysaccharide, so that oligosaccharides with low molecular weight are generated to provide a carbon source for self growth. The reduced end resulting from the cleavage of the glycosidic bond can be detected by the DNS method. The growth curve of the strain in a culture medium with enteromorpha polysaccharide as a unique carbon source and the reducing sugar content curve chart in fermentation supernatant are shown in figure 4. The concentration of the growth bacteria of vibrio H11 is found to be highest at 14H, which is consistent with the content change of reducing sugar in the culture medium. But the residual reducing sugar content in the fermentation liquor can not directly reflect the enzyme activity of the crude enzyme liquid. Therefore, the fermentation supernatant of the strain is used as a crude enzyme solution to react with a 1% enteromorpha polysaccharide solution, and the enzyme activity (U/mL) of the crude enzyme solution at different fermentation times is measured, and the result is shown in FIG. 5. Before 12h, the enzyme activity of the crude enzyme liquid in the fermentation liquid is gradually increased along with the gradual increase of the fermentation time, the enzyme activity is highest at 12h, and the enzyme activity is slightly reduced after the 12 h.
(3) Optimized culture of Vibrio sp.H11
In order to improve the enzyme activity of the crude enzyme liquid in the fermentation liquid, the culture medium formula and the culture conditions of the vibrio H11 are optimized. The enteromorpha polysaccharide is grown by taking the enteromorpha polysaccharide as a unique carbon source, and the optimized culture medium comprises (1L): (NH)4)2SO4，1.0g；Na2HPO4，0.8g；KH2PO4，0.2g；MgSO4·7H2O，0.2g； CaCl2·2H2O，0.1g；FeCl3·6H2O，5mg；(NH4)6Mo7O24·4H2O，1mg；ddH2O1L, pH 8.71; the culture temperature was 30 ℃.
After the conditions are optimized, the strain is cultured under the optimized conditions, the enzyme activity is measured, and the enzyme activity can reach 8.43U/mL and is obviously higher than the enzyme activity before optimization. Table 2 shows the comparison of the hydrolysis capacities of the strains related to hydrolysis of Enteromorpha prolifera polysaccharide and the vibrio H11 of the invention on the Enteromorpha prolifera polysaccharide. Wherein the crude enzyme solution of Vibrio sp.H11 has higher enzyme activity than the corresponding enzymes of single strain Alteromonas sp.A321 and Alteromonas macleodii B7; meanwhile, the activity of the enzyme is higher than that of Micro-community D2-1 of the mixed bacterial population. Although the enzyme activity is lower than that of Micro-immunity H1, Vibrio sp.H11 is a single strain, and is more stable and easier to store than the strain; micro-community H1 is prone to change the flora structure during the culture process.
TABLE 2 comparison of Enteromorpha polysaccharide hydrolysis ability of different strains (groups)
(4) Application of Vibrio sp.H11
The crude enzyme solution is obtained after the formula and the culture conditions of the culture medium are optimized, and acts on the anaerobic fermentation culture medium (containing no reducing agent) of enteromorpha powder and enteromorpha polysaccharide with the concentration of 20g/L (2 percent) and 50 g/L (5 percent). The strain H11 of the invention was cultured under optimized culture conditions and samples were taken at 26H of fermentation. In order to obtain more enzyme amount of the crude enzyme solution, enteromorpha polysaccharide or enteromorpha powder is fully hydrolyzed, the crude enzyme solution is ultrafiltered and concentrated at 4 ℃ to collect the crude enzyme solution, and the enzyme activity of the crude enzyme solution is measured. Adding concentrated crude enzyme solution (50ml of Enteromorpha powder or Enteromorpha polysaccharide anaerobic fermentation culture medium (without reducing agent) solution into the anaerobic fermentation culture medium), and hydrolyzing in a shaker at 40 deg.C and 200rpm for 48 h. Then inoculating Clostridium acetobutylicum WA strain for anaerobic fermentation.
When the content of the enteromorpha powder is 2%, the generated biological hydrogen amount is 1553.4mL/L, and the concentration of the biological solvent is 3.46 g/L. When the content of the enteromorpha powder is 5%, the generated biogas amount is 1682mL/L, and the bio-solvent amount is 4.53 g/L. The higher the concentration of the enteromorpha powder is, the higher the yield of hydrogen and the biological solvent is. When the content of the enteromorpha polysaccharide is 2%, the generated biological hydrogen amount is 1473.4mL/L, and the biological solvent amount is 4.77 g/L; when the content of the enteromorpha polysaccharide is 5%, the generated biological hydrogen amount is 3217 mL/L, and the biological solvent amount is 6.74 g/L. The higher the concentration of the enteromorpha polysaccharide is, the higher the yield of hydrogen and a biological solvent is. Meanwhile, comparing the enteromorpha powder and the enteromorpha polysaccharide, the enteromorpha polysaccharide generates more hydrogen and biological solvent under the same concentration. The results are shown in FIG. 6, FIG. 7 and FIG. 8. Through comprehensive comparative analysis, the enteromorpha polysaccharide has the value of producing biofuel.
For the hydrolysis of the enteromorpha polysaccharide, the mixed flora has better hydrolysis capacity, but the flora composition is easy to change and is difficult to store. The strain of Vibrio sp.H11 in the invention is used as a single strain, and the enteromorpha polysaccharide hydrolysis capability of the strain is strongest.
SEQUENCE LISTING
<110> Shantou university
<120> Vibrio H11 and uses thereof
<130> 2018
<160> 3
<170> PatentIn version 3.3
<210> 1
<211> 1385
<212> DNA
<213> Artificial sequence
<400> 1
tcttttgcag cccactccca tggtgtgacg ggcggtgtgt acaaggcccg ggaacgtatt 60
caccgtggca ttctgatcca cgattactag cgattccgac ttcatggagt cgagttgcag 120
actccaatcc ggactacgac gcactttttg ggattcgctc actttcgcaa gttggccgcc 180
ctctgtatgc gccattgtag cacgtgtgta gccctactcg taagggccat gatgacttga 240
cgtcgtcccc accttcctcc ggtttatcac cggcagtctc cctggagttc ccgactttac 300
tcgctggcaa acaaggataa gggttgcgct cgttgcggga cttaacccaa catttcacaa 360
cacgagctga cgacagccat gcagcacctg tctctcagtt cccgaaggca caagactgtc 420
tccagtctct tctgaggatg tcaagagtag gtaaggttct tcgcgttgca tcgaattaaa 480
ccacatgctc caccgcttgt gcgggccccc gtcaattcat ttgagtttta atcttgcgac 540
cgtactcccc aggcggtcta cttaacgcgt tagctccgaa agccacggct caaggccaca 600
acctccaagt agacatcgtt gacggcgtgg actaccaggg tatctaatcc tgtttgctcc 660
ccacgctttc gcatctgagt gtcagtatct gtccaggggg ccgccttcgc caccggtatt 720
ccttcagatc tctacgcatt tcaccgctac acctgaaatt ctacccccct ctacagtact 780
ctagcctgcc agtttcaaat gcaattccga ggttgagccc cgggctttca catctgactt 840
aacaaaccac ctgcatgcgc tttacgccca gtaattccga ttaacgctcg caccctccgt 900
attaccgcgg ctgctggcac ggagttagcc ggtgcttctt ctgcagctaa cgtcaaacga 960
atgcgctatt aacacatccg ccttcctcac tgctgaaagt actttacaac ccgaaggcct 1020
tcttcataca cgcggcatgg ctgcatcagg cttgcgccca ttgtgcaata ttccccactg 1080
ctgcctcccg taggagtctg gaccgtgtct cagttccagt gtggctgatc atcctctcag 1140
accagctagg gatcgtcgcc ttggtgagcc cttacctcac caactagcta atcccacctg 1200
ggcatatcct gacgcgagag gcccgaaggt ccccctcttt ggcccgaagg catcatgcgg 1260
tattagccat cgtttccaat ggttatcccc cacatcaggg caatttccca ggcattactc 1320
acccgtccgc cgctcgccgc ccttaacgtt ccccgaaggt tcagttaagt cgctgccgct 1380
cgact 1385
<210> 2
<211> 20
<212> DNA
<213> Artificial sequence
<400> 2
<210> 3
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<212> DNA
<213> Artificial sequence
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Claims (3)
1. Vibrio H11(Vibrio sp, H11), characterized in that it is used for hydrolysing "green tide" biomass, hydrolysing enteromorpha into clostridial fermentable reducing sugars; the vibrio H11 hydrolyzes enteromorpha into reducing sugar, and then is converted into a biological energy substance by combining anaerobic fermentation of clostridium; the vibrio H11 is preserved in China center for type culture Collection with the preservation number of CCTCC NO: m2018172.
2. The use of the vibrio H11 according to claim 1, wherein the vibrio H11 is cultured by inoculating the vibrio H11 strain to a medium with enteromorpha polysaccharide as a sole carbon source, and the medium consists of (NH)4)2SO4，1.0 g；Na2HPO4，0.8 g；KH2PO4，0.2 g；MgSO4·7H2O，0.2 g；CaCl2·2H2O，0.1 g；FeCl3·6H2O，5 mg；(NH4)6Mo7O24·4H2O，1 mg；ddH2O1L; 20g of enteromorpha polysaccharide; tryptone, 5 g; the pH was 8.71; the culture was carried out at 30 ℃ for 12 h.
3. The use of Vibrio H11 according to claim 1, wherein the method of use comprises: adding concentrated crude enzyme solution containing vibrio H11 into anaerobic fermentation culture medium containing enteromorpha powder or enteromorpha polysaccharide, hydrolyzing in a shaker at 40 deg.C and 200rpm for 48H, and inoculating clostridium for anaerobic fermentation.
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| CN114410542B (en) * | 2022-02-15 | 2023-08-11 | 青岛中尚奇生物科技有限公司 | Vibrio paradise and application thereof in degradation of enteromorpha |
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Non-Patent Citations (3)
| Title |
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| Biodegradation of Enteromorpha polysaccharides by intestinal micro-community from Siganus oramin;Zhang Zhibiao等;《JOURNAL OF OCEAN UNIVERSITY OF CHINA》;20161231;第15卷(第6期);1034-1038 * |
| 源自黄斑篮子鱼肠道菌群的B30菌株降解浒苔多糖的活性研究;谢罗瀚等;《韩山师范学院学报》;20161231;第37卷(第6期);49-55 * |
| 篮子鱼肠道来源浒苔多糖降解菌的研究;张志标等;《广东省遗传学会第九届代表大会暨学术研讨会论文及摘要汇编》;20141219;222 * |
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