CN108753635A - A kind of artificial culture method of liquid yeast starter - Google Patents
A kind of artificial culture method of liquid yeast starter Download PDFInfo
- Publication number
- CN108753635A CN108753635A CN201810896873.3A CN201810896873A CN108753635A CN 108753635 A CN108753635 A CN 108753635A CN 201810896873 A CN201810896873 A CN 201810896873A CN 108753635 A CN108753635 A CN 108753635A
- Authority
- CN
- China
- Prior art keywords
- culture method
- artificial culture
- wheat
- sorghum
- added
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Disclosed by the invention to belong to brewing technical field, specially a kind of artificial culture method of liquid yeast starter, the artificial culture method of the liquid yeast starter includes the following steps：S1：Feedstock processing：8-12 part of 35-45 parts of wheat and sorghum are taken, wheat and sorghum are ground into powdered, then addition water stirs evenly, material moistening 50-70 minutes；S2：Impregnate heating；S3：Cooling：Water purification is added, is cooled to 28-30 degrees Celsius, carbohydrase and active dry yeast is then added, stirs evenly；S4：Expand culture：Carry out heat preservation activation and proliferation culture, it is primary every stirring in 3.5-4.5 hours, and it is aerated processing in stirring, a kind of artificial culture method for liquid yeast starter which proposes, it can economize on resources, cost is reduced, the reproductive efficiency of saccharomycete can be greatly improved, and yeast is healthy and strong, bud life rate is high, miscellaneous bacteria rate is low with the death rate, which improves the utilization ratio of raw material, with good economic efficiency.
Description
Technical field
The present invention relates to brewing technical field, specially a kind of artificial culture method of liquid yeast starter.
Background technology
Alcoholic fermentation is the process that the sugar of fermentability is converted to alcohol by saccharomycete.Distillery yeast belongs on taxology
Eumycota, sac fungus is rigid, Protoascomycetae, Endomycetale, Endomycetaceae, yeast subfamily, saccharomyces, is weight in fermentation industry
The microorganism wanted, can the fermentable carbohydrates generation substances such as alcohol and carbon dioxide.Saccharomycete is commonly called as wine in Alcohol Production
Mother, and the expansion incubation of saccharomycete is exactly the preparation process of distiller's yeast.The purpose that distiller's yeast expands culture is from a small amount of yeast
A large amount of yeast cells are bred, required yeast starter when fermenting with supply.The use of the cultural method yeast of existing distiller's yeast
Measure larger, cost is excessively high, and the reproductive efficiency of saccharomycete and transfer efficiency are low.For this purpose, it is proposed that a kind of people of liquid yeast starter
Work cultural method.
Invention content
The purpose of the present invention is to provide a kind of artificial culture methods of liquid yeast starter, to solve to carry in above-mentioned background technology
The usage amount of the cultural method yeast of the existing distiller's yeast gone out is larger, and cost is excessively high, and the reproductive efficiency of saccharomycete and conversion are imitated
The low problem of rate.
To achieve the above object, the present invention provides the following technical solutions：A kind of artificial culture method of liquid yeast starter, the liquid
The artificial culture method of body distiller's yeast includes the following steps：
S1：Feedstock processing：8-12 part of 35-45 parts of wheat and sorghum are taken, wheat and sorghum are ground into powdered, is then added
Enter water to stir evenly, material moistening 50-70 minutes；
S2：Impregnate heating：It is soaked in wheat and sorghum flour, then heating is boiled under normal pressure, and keeps 20-30
Minute, stop heating, room temperature 1-3 hours；
S3：Cooling：Water purification is added, is cooled to 28-30 degrees Celsius, carbohydrase and active dry yeast is then added, stirring is equal
It is even；
S4：Expand culture：Carry out heat preservation activation and proliferation culture, it is primary every stirring in 3.5-4.5 hour, and when stirring into
Row ventilation process is cultivated 22-25 hours.
Preferably, the PH of the water in the step S1 is 4.5-5.
Preferably, in the step S1, add it is water-moistened during need be added rice husk, and the dosage of rice husk be wheat
With the 18%-20% of sorghum.
Preferably, the PH of the water purification in the step S3 is 4.2-4.8.
Preferably, the carbohydrase in the step S3 and active dry yeast need to temper before addition, and carbohydrase
Dosage with active dry yeast is the 0.6%-0.8% of raw material, and the weight ratio between carbohydrase and active dry yeast is 5:2.
Compared with prior art, the beneficial effects of the invention are as follows：A kind of artificial culture for liquid yeast starter that the invention proposes
Method can economize on resources, reduce cost, can greatly improve the reproductive efficiency of saccharomycete, and yeast is healthy and strong, and bud life rate is high,
Miscellaneous bacteria rate is low with the death rate, which improves the utilization ratio of raw material, with good economic efficiency.
Description of the drawings
Fig. 1 is structure of the invention artificial culture method flow chart.
Specific implementation mode
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation describes, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
Referring to Fig. 1, the present invention provides a kind of artificial culture method of liquid yeast starter：
Embodiment 1
The artificial culture method of the liquid yeast starter includes the following steps：
S1：Feedstock processing：12 parts of 35 parts of wheat and sorghum are taken, wheat and sorghum are ground into powdered, water is then added
Stir evenly, material moistening 70 minutes, the PH of water is 5, add it is water-moistened during need that rice husk is added, and the dosage of rice husk is small
The 20% of wheat and sorghum；
S2：Impregnate heating：It is soaked in wheat and sorghum flour, then heating is boiled under normal pressure, and is kept for 30 points
Clock stops heating, room temperature 3 hours；
S3：Cooling：Water purification is added, is cooled to 30 degrees Celsius, carbohydrase and active dry yeast is then added, stirs evenly,
The PH of water purification is 4.8, and carbohydrase and active dry yeast need to temper before addition, and the use of carbohydrase and active dry yeast
Amount is the 0.8% of raw material, and the weight ratio between carbohydrase and active dry yeast is 5:2；
S4：Expand culture：Heat preservation activation and proliferation culture is carried out, it is primary every stirring in 4.5 hours, and led in stirring
Wind processing, is cultivated 25 hours.
Embodiment 2
The artificial culture method of the liquid yeast starter includes the following steps：
S1：Feedstock processing：8 parts of 45 parts of wheat and sorghum are taken, wheat and sorghum are ground into powdered, water is then added and stirs
Mix uniformly, material moistening 50 minutes, the PH of water is 4.5, add it is water-moistened during need that rice husk is added, and the dosage of rice husk is small
The 18% of wheat and sorghum；
S2：Impregnate heating：It is soaked in wheat and sorghum flour, then heating is boiled under normal pressure, and is kept for 20 points
Clock stops heating, room temperature 1 hour；
S3：Cooling：Water purification is added, is cooled to 28 degrees Celsius, carbohydrase and active dry yeast is then added, stirs evenly,
The PH of water purification is 4.2, and carbohydrase and active dry yeast need to temper before addition, and the use of carbohydrase and active dry yeast
Amount is the 0.6% of raw material, and the weight ratio between carbohydrase and active dry yeast is 5:2；
S4：Expand culture：Heat preservation activation and proliferation culture is carried out, it is primary every stirring in 3.5 hours, and led in stirring
Wind processing, is cultivated 22 hours.
Embodiment 3
The artificial culture method of the liquid yeast starter includes the following steps：
S1：Feedstock processing：10 parts of 40 parts of wheat and sorghum are taken, wheat and sorghum are ground into powdered, water is then added
Stir evenly, material moistening 60 minutes, the PH of water is 4.75, add it is water-moistened during need that rice husk, and the dosage of rice husk is added
It is the 19% of wheat and sorghum；
S2：Impregnate heating：It is soaked in wheat and sorghum flour, then heating is boiled under normal pressure, and is kept for 25 points
Clock stops heating, room temperature 2 hours；
S3：Cooling：Water purification is added, is cooled to 29 degrees Celsius, carbohydrase and active dry yeast is then added, stirs evenly,
The PH of water purification is 4.6, and carbohydrase and active dry yeast need to temper before addition, and the use of carbohydrase and active dry yeast
Amount is the 0.7% of raw material, and the weight ratio between carbohydrase and active dry yeast is 5:2；
S4：Expand culture：Heat preservation activation and proliferation culture is carried out, it is primary every stirring in 4 hours, and be aerated in stirring
Processing is cultivated 24 hours.
The liquid yeast starter that above three embodiments are cultivated is detected, liquid yeast starter is milky slightly with micro- Huang
Color, and viable count >=1.2 × 109/ml。
When in use, the usage amount of liquid yeast starter is the 0.5%-1.5% of inventory, the artificial culture side of the liquid yeast starter
Method can economize on resources, reduce cost, can greatly improve the reproductive efficiency of saccharomycete, and yeast is healthy and strong, bud life rate is high, miscellaneous
Bacterium rate is low with the death rate, which improves the utilization ratio of raw material, with good economic efficiency.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
Understanding without departing from the principles and spirit of the present invention can carry out these embodiments a variety of variations, modification, replace
And modification, the scope of the present invention is defined by the appended.
Claims (5)
1. a kind of artificial culture method of liquid yeast starter, it is characterised in that：The artificial culture method of the liquid yeast starter includes as follows
Step：
S1：Feedstock processing：8-12 parts of 35-45 parts of wheat and sorghum are taken, wheat and sorghum are ground into powdered, water is then added
It stirs evenly, material moistening 50-70 minutes；
S2：Impregnate heating：It is soaked in wheat and sorghum flour, then heating is boiled under normal pressure, and is kept for 20-30 points
Clock stops heating, room temperature 1-3 hours；
S3：Cooling：Water purification is added, is cooled to 28-30 degrees Celsius, carbohydrase and active dry yeast is then added, stirs evenly；
S4：Expand culture：Heat preservation activation and proliferation culture is carried out, it is primary every stirring in 3.5-4.5 hours, and led in stirring
Wind processing, is cultivated 22-25 hours.
2. a kind of artificial culture method of liquid yeast starter according to claim 1, it is characterised in that：In the step S1
The PH of water is 4.5-5.
3. a kind of artificial culture method of liquid yeast starter according to claim 1, it is characterised in that：In the step S1,
Add it is water-moistened during need be added rice husk, and the dosage of rice husk be wheat and sorghum 18%-20%.
4. a kind of artificial culture method of liquid yeast starter according to claim 1, it is characterised in that：In the step S3
The PH of water purification is 4.2-4.8.
5. a kind of artificial culture method of liquid yeast starter according to claim 1, it is characterised in that：In the step S3
Carbohydrase and active dry yeast need to temper before addition, and the dosage of carbohydrase and active dry yeast is raw material
0.6%-0.8%, and the weight ratio between carbohydrase and active dry yeast is 5:2.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810896873.3A CN108753635A (en) | 2018-08-08 | 2018-08-08 | A kind of artificial culture method of liquid yeast starter |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810896873.3A CN108753635A (en) | 2018-08-08 | 2018-08-08 | A kind of artificial culture method of liquid yeast starter |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108753635A true CN108753635A (en) | 2018-11-06 |
Family
ID=63969317
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810896873.3A Pending CN108753635A (en) | 2018-08-08 | 2018-08-08 | A kind of artificial culture method of liquid yeast starter |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108753635A (en) |
-
2018
- 2018-08-08 CN CN201810896873.3A patent/CN108753635A/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Appiah-Nkansah et al. | Ethanol production from mixtures of sweet sorghum juice and sorghum starch using very high gravity fermentation with urea supplementation | |
| CN104774876B (en) | A kind of method of lignocellulose biomass comprehensive utilization | |
| US20200172938A1 (en) | Method for domesticating saccharomyces cerevisiae | |
| JP5412272B2 (en) | Nutrients for simultaneous saccharification-fermentation culture media for ethanol production | |
| CN104774877B (en) | A kind of method of lignocellulose biomass co-producing ethanol, acetone and butanol | |
| CN101381665A (en) | Complex enzyme and use thereof | |
| CN1970725B (en) | Method and device for preparing Shanxi mellow vinegar by liquid-solid technology | |
| CN110564545A (en) | method for improving grain mash stacking saccharification efficiency of Xiaoqu liquor by utilizing rhizopus koji | |
| CN103805582A (en) | Beta-glucanase containing special enzyme for growing pigs and preparation method thereof | |
| CN112205514A (en) | Method for producing multifunctional spirit vinasse feed | |
| CN110607246B (en) | Yeast high-density propagation vinasse polypeptide molasses culture medium and preparation method | |
| CN103898166A (en) | Method of producing ethanol | |
| Liu et al. | Bioethanol production from potato starch by a novel vertical mass-flow type bioreactor with a co-cultured-cell strategy | |
| CN103461654A (en) | Method for producing vinasse protein feed from corn steep liquor | |
| CN104560505A (en) | Process for brewing yellow wine by feeding starch and glucose liquid | |
| CN107455723A (en) | A kind of method for the cooking wine that homoamino acid state nitrogen content is produced using lees of yellow wine | |
| CN105779328A (en) | Method for culturing clostridium thermocellum | |
| CN110106107A (en) | A kind of caproic acid bacteria mixed culturing method | |
| CN108753635A (en) | A kind of artificial culture method of liquid yeast starter | |
| CN107779479B (en) | Saccharomyces cerevisiae composition for producing ethanol and method for producing ethanol by using saccharomyces cerevisiae composition | |
| CN104862167B (en) | It is a kind of to control the method that acid puies forward effect suitable for the Daqu delicate fragrance white wine hot season | |
| CN101177695B (en) | High-concentration alcoholic fermentation method | |
| CN103820419B (en) | A kind of preparation method of the good beta glucan enzyme of heat endurance | |
| CN111690707A (en) | Method and production system for preparing humus and artificial aged pit mud by waste vinasse double-round fermentation | |
| JP2010227074A (en) | Method for production of ethanol by continuous fermentation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20181106 |
|
| WD01 | Invention patent application deemed withdrawn after publication |