CN108751767A - A kind of microorganism foaming agent prepared for foamed concrete - Google Patents

A kind of microorganism foaming agent prepared for foamed concrete Download PDF

Info

Publication number
CN108751767A
CN108751767A CN201810394370.6A CN201810394370A CN108751767A CN 108751767 A CN108751767 A CN 108751767A CN 201810394370 A CN201810394370 A CN 201810394370A CN 108751767 A CN108751767 A CN 108751767A
Authority
CN
China
Prior art keywords
foaming agent
liquid
microorganism
barms
foamed concrete
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201810394370.6A
Other languages
Chinese (zh)
Inventor
荣辉
张静
张磊
张颖
徐蕊
王海良
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tianjin Chengjian University
Original Assignee
Tianjin Chengjian University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tianjin Chengjian University filed Critical Tianjin Chengjian University
Priority to CN201810394370.6A priority Critical patent/CN108751767A/en
Publication of CN108751767A publication Critical patent/CN108751767A/en
Pending legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C04CEMENTS; CONCRETE; ARTIFICIAL STONE; CERAMICS; REFRACTORIES
    • C04BLIME, MAGNESIA; SLAG; CEMENTS; COMPOSITIONS THEREOF, e.g. MORTARS, CONCRETE OR LIKE BUILDING MATERIALS; ARTIFICIAL STONE; CERAMICS; REFRACTORIES; TREATMENT OF NATURAL STONE
    • C04B40/00Processes, in general, for influencing or modifying the properties of mortars, concrete or artificial stone compositions, e.g. their setting or hardening ability
    • C04B40/0028Aspects relating to the mixing step of the mortar preparation
    • C04B40/0039Premixtures of ingredients
    • C04B40/0046Premixtures of ingredients characterised by their processing, e.g. sequence of mixing the ingredients when preparing the premixtures
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • C12N1/16Yeasts; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C04CEMENTS; CONCRETE; ARTIFICIAL STONE; CERAMICS; REFRACTORIES
    • C04BLIME, MAGNESIA; SLAG; CEMENTS; COMPOSITIONS THEREOF, e.g. MORTARS, CONCRETE OR LIKE BUILDING MATERIALS; ARTIFICIAL STONE; CERAMICS; REFRACTORIES; TREATMENT OF NATURAL STONE
    • C04B2103/00Function or property of ingredients for mortars, concrete or artificial stone
    • C04B2103/42Pore formers

Abstract

The invention discloses a kind of microorganism foaming agent prepared for foamed concrete, the mass percentage composition of microorganism foaming agent is:Glucose:10%-15%;Peptone:10%-20%;Bacterium soaks powder:5%-10%;Thin strain liquid:5%-10%;Foam stabilizer:0.2%-1%;The thin strain liquid is barms liquid, is prepared by the following:Yeast strain after activation is inoculated in basal medium, barms liquid is made in shaken cultivation in 35 DEG C, the constant incubator of 170r/min after 24 hours;Microorganism foaming agent of the present invention is nontoxic, and gas production is big and stablizes, and every gram of foaming agent can generate the CO of 200cc or more2Gas, the required raw material sources of preparation are extensive, simple and easy to get, cheap.

Description

A kind of microorganism foaming agent prepared for foamed concrete
Technical field
The present invention relates to a kind of foaming agents prepared for foamed concrete, more particularly to a kind of to be used for foamed concrete system Standby microorganism foaming agent.
Background technology
With the popularization and application of China's building energy conservation, foam concrete is good with its lightweight, insulation, fire prevention, shock resistance Equal excellent performances become novel environment friendly structural material.In the preparation of foam concrete, the performance of foaming agent is to foam concrete Performance influence it is very big, directly affect the mobility and stability of cement-based material.
Foaming agent for foam concrete is there are many kinds of class, such as:Rosin tree lipid foaming agent, protein-based foaming agent, Synthesize class foaming agent, complex class foaming agent.Rosin tree lipid foaming agent foam stability and coefficient of foaming are poor, and rosin heat Polymers foaming agent also contains toxic phenol etc., is adversely affected to environment.Synthesize foaming agent such as anion surfactant Equally exist the problem that foaming capacity is low, foam stability is poor.Using animal keratoprotein as the protein-based foaming agent of raw material Be widely recognized as both at home and abroad, but the keratoprotein resource as foaming agent is limited, and this kind of foaming agent production cycle it is long, at This height, environmental pollution are big, and exploitation receives restriction with application.Complex class foaming agent because its foaming capacity and foam stability compared with Well and gradually instead of other types foaming agent, but lack economy.
Invention content
In face of above-mentioned variety of problems, the present inventor is by largely orienting the foaming research to microorganism, by the data of gained It is depicted as curve, it was found that the gas happening law of saccharomycete obtains the microorganism foaming agent that a kind of gas production is big and stablizes finally.
The object of the present invention is to provide a kind of microorganism foaming agents prepared for foamed concrete.
The mass percentage composition of microorganism foaming agent of the present invention is:Glucose:10%-15%;Peptone:10%- 20%;Bacterium soaks powder:5%-10%;Thin strain liquid:5%-10%;Foam stabilizer:0.2%-1%.
The bacterium leaching powder is yeast extract;
The foam stabilizer is the mixture of one or more of gelatin, Arabic gum, xanthans or cellulose etc.;
The thin strain liquid is barms liquid, is prepared by the following:
Yeast strain after activation is inoculated in basal medium, is shaken in 35 DEG C, the constant incubator of 170r/min Barms liquid is made after swinging culture 24 hours.
Microorganism foaming agent of the present invention can obtain by the following method, including step:
1) glucose, peptone, yeast extract are put into conical flask and prepare culture medium solution;
2) conical flask is sealed with gauze, is put into autoclave and sterilizes 25 minutes;After sterilizing, taking-up is positioned over sterile Operation console is sterilized 30 minutes, is cooled to room temperature;
3) barms liquid is seeded in culture medium solution with liquid-transfering gun, the solution after inoculation is put into 35 DEG C of insulating boxs Middle shaken cultivation 48 hours;The rotating speed after foam stabilizer with 3000r/min is added thereto to stir 30m minutes, obtains microorganism foaming Agent.
The advantages and features of the present invention:
1. compared with prior art, the outstanding feature of microorganism foaming agent of the present invention is to produce using common saccharomycete Angry body, it is nontoxic, there is no chemical blowing process foam is unstable and environmental pollution problem;
2. the gas production of microorganism foaming agent is big and stablizes, every gram of foaming agent can generate the CO of 200cc or more2Gas Body.
3.. prepare that microorganism foaming agent of the present invention energy consumption is low, and raw material sources are extensive, simple and easy to get, cheap.
Mechanism of the present invention is:
Substrate hydrolysis is generated gas by saccharomycete by its respiration.
It is aerobic:C6H12O6+O2→ other substances+6CO2;Anaerobic:C6H12O6→C2H5Other substances of OH++3CO2
Specific implementation mode
For a better understanding of the present invention.It is described further with reference to embodiment, but does not limit the present invention.
Yeast strain:It purchases from China General Microbiological culture presevation administrative center
Yeast extract:It purchases from extensive and profound in meaning star Bioisystech Co., Ltd
Gelatin:Particle is purchased from the Tianjin chemical inc Bo Di
Embodiment 1
Prepare barms liquid:
Yeast strain after activation is inoculated in the 250mL conical flasks of the basal medium containing 100mL, at 35 DEG C, 170r/ Shaken cultivation obtains barms liquid in the constant incubator of min after 24 hours.
Prepare microorganism foaming agent:
Take 10g glucose, 10g peptones, 5g yeast extracts, 10g barms liquid, 0.2g gelatin.By glucose, albumen Peptone, yeast extract are placed in conical flask, are configured to 100 milliliters of culture medium solutions;Then taper bottle closure is put into and is gone out with gauze It sterilizes 25 minutes in bacterium pot;It is taken out after sterilizing and is positioned over aseptic operating platform, sterilized 30 minutes, be cooled to room temperature;Use liquid relief Solution after inoculation inoculation yeast strain liquid and is put into shaken cultivation 48 hours in 35 DEG C of insulating boxs by rifle in culture medium solution; Gelatin is added thereto later, and is stirred 30m minutes with the rotating speed of 3000r/min, obtains microorganism foaming agent.Gained microorganism sends out The gas production of infusion is up to 295mL.
Embodiment 2
10g glucose, 15g peptones, 10g yeast extracts, 5g barms liquid, 0.4g gelatin are taken, is grasped by embodiment 1 Make, obtains microorganism foaming agent.
Embodiment 3
15g glucose, 15g peptones, 5g yeast extracts, 15g barms liquid, 1g gelatin are taken, is operated by embodiment 1, Obtain microorganism foaming agent.

Claims (2)

1. a kind of microorganism foaming agent prepared for foamed concrete, it is characterised in that its mass percentage composition is:Grape Sugar:10%-15%;Peptone:10%-20%;Bacterium soaks powder:5%-10%;Thin strain liquid:5%-10%;Foam stabilizer: 0.2%-1%.
The bacterium leaching powder is yeast extract;
The foam stabilizer is the mixture of one or more of gelatin, Arabic gum, xanthans or cellulose etc.;
The thin strain liquid is barms liquid, is prepared by the following:
Yeast strain after activation is inoculated in basal medium, the oscillation training in 35 DEG C, the constant incubator of 170r/min Barms liquid is made after supporting 24 hours.
2. a kind of preparation method of microorganism foaming agent prepared for foamed concrete as described in claim 1, feature It is to include step:
1) glucose, peptone, yeast extract are put into conical flask and prepare culture medium solution;
2) conical flask is sealed with gauze, is put into autoclave and sterilizes 25 minutes;After sterilizing, taking-up is positioned over sterile working Platform is sterilized 30 minutes, is cooled to room temperature;
3) barms liquid is seeded in culture medium solution with liquid-transfering gun, the solution after inoculation is put into 35 DEG C of insulating boxs and is shaken Swing culture 48 hours;The rotating speed after foam stabilizer with 3000r/min is added thereto to stir 30m minutes, obtains microorganism foaming agent.
CN201810394370.6A 2018-04-27 2018-04-27 A kind of microorganism foaming agent prepared for foamed concrete Pending CN108751767A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201810394370.6A CN108751767A (en) 2018-04-27 2018-04-27 A kind of microorganism foaming agent prepared for foamed concrete

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201810394370.6A CN108751767A (en) 2018-04-27 2018-04-27 A kind of microorganism foaming agent prepared for foamed concrete

Publications (1)

Publication Number Publication Date
CN108751767A true CN108751767A (en) 2018-11-06

Family

ID=64012420

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201810394370.6A Pending CN108751767A (en) 2018-04-27 2018-04-27 A kind of microorganism foaming agent prepared for foamed concrete

Country Status (1)

Country Link
CN (1) CN108751767A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109574533A (en) * 2018-12-20 2019-04-05 贵州安凯达实业股份有限公司 A kind of environmentally friendly foaming agent and preparation method thereof
CN109704624A (en) * 2018-11-27 2019-05-03 天津城建大学 A kind of microorganism foaming agent and preparation method for foamed concrete preparation
CN112979339A (en) * 2021-02-08 2021-06-18 天津城建大学 Microbial foaming raw soil composition and preparation method of building material based on same

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2205162C2 (en) * 2001-11-23 2003-05-27 Винаров Александр Юрьевич Cellular concrete production method using protein foaming agent
CN101591155A (en) * 2009-06-18 2009-12-02 西南科技大学 Yeast protein concrete foaming agent and preparation method thereof
CN107311562A (en) * 2017-06-26 2017-11-03 河南理工大学 A kind of wheat stalk fermentation concrete segment and preparation method thereof
CN107673696A (en) * 2017-10-26 2018-02-09 陈宗熙 A kind of foam concrete self-insulating wall material and preparation method thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2205162C2 (en) * 2001-11-23 2003-05-27 Винаров Александр Юрьевич Cellular concrete production method using protein foaming agent
CN101591155A (en) * 2009-06-18 2009-12-02 西南科技大学 Yeast protein concrete foaming agent and preparation method thereof
CN107311562A (en) * 2017-06-26 2017-11-03 河南理工大学 A kind of wheat stalk fermentation concrete segment and preparation method thereof
CN107673696A (en) * 2017-10-26 2018-02-09 陈宗熙 A kind of foam concrete self-insulating wall material and preparation method thereof

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
刘佳奇等: ""一种新型蛋白质混凝土发泡剂的研制及性能研究"", 《现代化工》 *
王程等: ""改性酵母蛋白制备混凝土发泡剂及其处理条件优化"", 《化工进展》 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109704624A (en) * 2018-11-27 2019-05-03 天津城建大学 A kind of microorganism foaming agent and preparation method for foamed concrete preparation
CN109574533A (en) * 2018-12-20 2019-04-05 贵州安凯达实业股份有限公司 A kind of environmentally friendly foaming agent and preparation method thereof
CN112979339A (en) * 2021-02-08 2021-06-18 天津城建大学 Microbial foaming raw soil composition and preparation method of building material based on same

Similar Documents

Publication Publication Date Title
CN108751767A (en) A kind of microorganism foaming agent prepared for foamed concrete
CN102268466A (en) Method for fermentation production of lipstatin and culture medium components thereof
Wang et al. Efficient production of L-sorbose from D-sorbitol by whole cell immobilization of Gluconobacter oxydans WSH-003
CN110331179B (en) Green synthesis method of biological dust suppressant
CN106747176A (en) A kind of novel gelled material and its preparation and application method with carbon sequestration capacity
JP7222911B2 (en) How to make pullulan
CA1117048A (en) Bacterial polysaccharide
CN101914439A (en) Method for preparing microorganism agent for increasing production of oilfield microorganisms or protecting environment
CN110951714B (en) Hyaluronic acid hydrolase, coding sequence thereof and method for preparing oligomeric hyaluronate by using same
CN107586718B (en) Preparation method of oxygen sustained-release granules applied to microbial fermentation culture
CN108640572A (en) A method of preparing foam concrete using microorganism foaming agent
CN104894032A (en) Bacillus subtilis growth acceleration and endospore generation culture method
CN101696434A (en) New process for fermenting Beta-polymalic acid
CN113072599A (en) Method for preparing natural hesperidin by biological fermentation and extraction
CN111996136A (en) Pseudomonas fluorescens fermentation medium, culture method and application
CN102660049B (en) Method and device for preparing temperature-induced reversible color-changing bacterial cellulose composite membrane
CN111004788B (en) Pectin esterase and preparation method and application thereof
CN109704624A (en) A kind of microorganism foaming agent and preparation method for foamed concrete preparation
CN113337417B (en) Agrobacterium capable of efficiently degrading ethyl carbamate and application thereof
CN101555498B (en) Technique for producing 2-keto-D-gluconic acid by fermentation of gluconate
CN112725321A (en) Pectin lyase and preparation method and application thereof
CN104804717B (en) A kind of foam blocking agent
CN112280812A (en) Method for improving fermentation yield of aureomycin A and ratio of aureomycin A to aureomycin B
CN106868063A (en) A kind of technique of microorganism fermenting propionic acid and its salt
CN105255969B (en) A method of improving hyaluronic acid volume of production of fermentation production

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
WD01 Invention patent application deemed withdrawn after publication
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20181106