CN108751126A - The method for preparing three-dimensional self-supporting film based on lysozyme nano thin-film - Google Patents
The method for preparing three-dimensional self-supporting film based on lysozyme nano thin-film Download PDFInfo
- Publication number
- CN108751126A CN108751126A CN201810587315.9A CN201810587315A CN108751126A CN 108751126 A CN108751126 A CN 108751126A CN 201810587315 A CN201810587315 A CN 201810587315A CN 108751126 A CN108751126 A CN 108751126A
- Authority
- CN
- China
- Prior art keywords
- lysozyme
- micro
- supporting film
- dimensional self
- nano granules
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B81—MICROSTRUCTURAL TECHNOLOGY
- B81C—PROCESSES OR APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OR TREATMENT OF MICROSTRUCTURAL DEVICES OR SYSTEMS
- B81C99/00—Subject matter not provided for in other groups of this subclass
-
- C—CHEMISTRY; METALLURGY
- C23—COATING METALLIC MATERIAL; COATING MATERIAL WITH METALLIC MATERIAL; CHEMICAL SURFACE TREATMENT; DIFFUSION TREATMENT OF METALLIC MATERIAL; COATING BY VACUUM EVAPORATION, BY SPUTTERING, BY ION IMPLANTATION OR BY CHEMICAL VAPOUR DEPOSITION, IN GENERAL; INHIBITING CORROSION OF METALLIC MATERIAL OR INCRUSTATION IN GENERAL
- C23C—COATING METALLIC MATERIAL; COATING MATERIAL WITH METALLIC MATERIAL; SURFACE TREATMENT OF METALLIC MATERIAL BY DIFFUSION INTO THE SURFACE, BY CHEMICAL CONVERSION OR SUBSTITUTION; COATING BY VACUUM EVAPORATION, BY SPUTTERING, BY ION IMPLANTATION OR BY CHEMICAL VAPOUR DEPOSITION, IN GENERAL
- C23C18/00—Chemical coating by decomposition of either liquid compounds or solutions of the coating forming compounds, without leaving reaction products of surface material in the coating; Contact plating
- C23C18/16—Chemical coating by decomposition of either liquid compounds or solutions of the coating forming compounds, without leaving reaction products of surface material in the coating; Contact plating by reduction or substitution, e.g. electroless plating
- C23C18/18—Pretreatment of the material to be coated
- C23C18/20—Pretreatment of the material to be coated of organic surfaces, e.g. resins
- C23C18/28—Sensitising or activating
- C23C18/30—Activating or accelerating or sensitising with palladium or other noble metal
-
- C—CHEMISTRY; METALLURGY
- C23—COATING METALLIC MATERIAL; COATING MATERIAL WITH METALLIC MATERIAL; CHEMICAL SURFACE TREATMENT; DIFFUSION TREATMENT OF METALLIC MATERIAL; COATING BY VACUUM EVAPORATION, BY SPUTTERING, BY ION IMPLANTATION OR BY CHEMICAL VAPOUR DEPOSITION, IN GENERAL; INHIBITING CORROSION OF METALLIC MATERIAL OR INCRUSTATION IN GENERAL
- C23C—COATING METALLIC MATERIAL; COATING MATERIAL WITH METALLIC MATERIAL; SURFACE TREATMENT OF METALLIC MATERIAL BY DIFFUSION INTO THE SURFACE, BY CHEMICAL CONVERSION OR SUBSTITUTION; COATING BY VACUUM EVAPORATION, BY SPUTTERING, BY ION IMPLANTATION OR BY CHEMICAL VAPOUR DEPOSITION, IN GENERAL
- C23C18/00—Chemical coating by decomposition of either liquid compounds or solutions of the coating forming compounds, without leaving reaction products of surface material in the coating; Contact plating
- C23C18/16—Chemical coating by decomposition of either liquid compounds or solutions of the coating forming compounds, without leaving reaction products of surface material in the coating; Contact plating by reduction or substitution, e.g. electroless plating
- C23C18/31—Coating with metals
- C23C18/38—Coating with copper
- C23C18/40—Coating with copper using reducing agents
- C23C18/405—Formaldehyde
-
- C—CHEMISTRY; METALLURGY
- C23—COATING METALLIC MATERIAL; COATING MATERIAL WITH METALLIC MATERIAL; CHEMICAL SURFACE TREATMENT; DIFFUSION TREATMENT OF METALLIC MATERIAL; COATING BY VACUUM EVAPORATION, BY SPUTTERING, BY ION IMPLANTATION OR BY CHEMICAL VAPOUR DEPOSITION, IN GENERAL; INHIBITING CORROSION OF METALLIC MATERIAL OR INCRUSTATION IN GENERAL
- C23C—COATING METALLIC MATERIAL; COATING MATERIAL WITH METALLIC MATERIAL; SURFACE TREATMENT OF METALLIC MATERIAL BY DIFFUSION INTO THE SURFACE, BY CHEMICAL CONVERSION OR SUBSTITUTION; COATING BY VACUUM EVAPORATION, BY SPUTTERING, BY ION IMPLANTATION OR BY CHEMICAL VAPOUR DEPOSITION, IN GENERAL
- C23C18/00—Chemical coating by decomposition of either liquid compounds or solutions of the coating forming compounds, without leaving reaction products of surface material in the coating; Contact plating
- C23C18/16—Chemical coating by decomposition of either liquid compounds or solutions of the coating forming compounds, without leaving reaction products of surface material in the coating; Contact plating by reduction or substitution, e.g. electroless plating
- C23C18/31—Coating with metals
- C23C18/42—Coating with noble metals
- C23C18/44—Coating with noble metals using reducing agents
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Materials Engineering (AREA)
- Mechanical Engineering (AREA)
- Metallurgy (AREA)
- Organic Chemistry (AREA)
- Microelectronics & Electronic Packaging (AREA)
- Medicinal Preparation (AREA)
- Manufacturing Of Micro-Capsules (AREA)
Abstract
The invention discloses a kind of methods preparing three-dimensional self-supporting film based on lysozyme nano thin-film, this method is using micro-nano granules as template, one layer of lysozyme nano thin-film is coated on its surface based on lysozyme phase transition, template is directly removed and can be obtained lysozyme three-dimensional self-supporting film；Also one layer of metallic can be deposited on the micro-nano granules surface that lysozyme coats or one layer of hydroxyapatite is grown on the micro-nano granules surface that lysozyme coats by biomineralization by electroless deposition processes, template is removed again, obtains metal three-dimensional self-supporting film or hydroxyapatite three-dimensional self-supporting film.Preparation process of the present invention is of short duration and simple easily operated, it is of low cost, environmentally protective simultaneously, it can be used for preparing organic, inorganic and metal class three-dimensional self-supporting film, with extensive universality, easily realize large-scale industrial application, and the size of gained three-dimensional self-supporting film, shape can be regulated and controled by template.
Description
Technical field
The present invention relates to it is a kind of it is environmentally protective, be simple and efficient and with extensive universality three-dimensional self-supporting film system
Preparation Method.
Background technology
Metal, inorganic or protein tridimensional capsule with special colloform texture and property are due to its space with isolation
With the multi-functional and potential application such as controllable permeability, in medicine/gene delivering, catalysis, sensor, medicine, cosmetics etc.
Have extensively using in different field and is particularly concerned.Manufacture can encapsulate the capsule of the micron and nano-scale of a variety of materials
(or shell) has the double meaning of Science and Technology.Up to the present, developed extensive hollow structure, as condensate,
Multilayer capsule and tiny balloon.The structure and form of capsule play a key role in terms of the property and function that determine capsule.
Although having there is the various methods for preparing three-dimensional capsule, most preparation method is complicated, with high costs, biocompatibility
It is poor, such as layer-by-layer (LBL), it is difficult to meet it is industrial there is an urgent need to.In another example in solid shell
Grain (core-shell particles) be widely used as microcapsules, for drug control release and targeting and for protect such as enzyme and
The sensitive agents such as protein.Such particle can be made by interfacial polymerization or by the phase separation of polymer-solvent mixture
It is standby.However, their manufacture faces many common problems, including the covering of polydispersity, non-uniform shell and core solidification.
The urgent need therefore it provides a kind of method that can be designed and make various types of other three-dimensional capsule seems.
Invention content
It is a kind of easy to operate, of low cost, green technical problem to be solved by the present invention lies in against the above deficiency, providing
The method for the three-dimensional self-supporting film of preparation that colour circle is protected.
Technical solution is made of following step used by solving above-mentioned technical problem：
1, the modification of micro-nano granules
The 4- hydroxyethyl piperazineethanesulfonic acid buffer solutions of 1~100mmol/L tri- (2- carboxyethyls) phosphine are adjusted to NaOH
PH value is 5.0~10.0, then mixes it with the 4- hydroxyethyl piperazineethanesulfonic acid buffer solutions of 1~50mg/mL lysozymes
It is even, then micro-nano granules are immersed in gained mixed liquor, it cultivates 20~60 minutes at room temperature, micro-nano granules surface is made to be formed
One layer of lysozyme nano thin-film, centrifugation, washing, obtains the micro-nano granules of lysozyme cladding.
2, three-dimensional self-supporting film is prepared
Micro-nano granules inside the micro-nano granules of lysozyme cladding are removed, it is thin to obtain lysozyme three-dimensional self-supporting
Film；Or by electroless deposition processes after micro-nano granules surface one layer of metallic of deposition that lysozyme coats, then remove it
Internal micro-nano granules, obtain metal three-dimensional self-supporting film；Or it is coated in lysozyme by biomineralization micro-nano
After particle surface grows one layer of hydroxyapatite, then its internal micro-nano granules is removed, obtains hydroxyapatite three-dimensional from branch
Support film.
In above-mentioned preparation method, the micro-nano granules are CaCO3When particle, in step 2, by lysozyme cladding
Micro-nano granules are immersed in 0.01~1mol/L disodium ethylene diamine tetra-acetic acid aqueous solutions, are cultivated 30~90 minutes, are obtained at room temperature
To lysozyme three-dimensional self-supporting film.
In above-mentioned preparation method, when the micro-nano granules are polystyrene microsphere, in step 2, by lysozyme packet
After the micro-nano granules area load palladium ion covered, then by electroless deposition processes one layer of metallic of deposition, then roasting removes
Micro-nano granules obtain metal three-dimensional self-supporting film, wherein the metal is copper or silver.
In above-mentioned preparation method, when the micro-nano granules are polystyrene microsphere, in step 2, by lysozyme packet
The micro-nano granules surface chelating Ca covered2+Afterwards, it immerses in simulated body fluid, is kept at 37 DEG C 7~10 days, then roasting removes micro-
Nano particle obtains hydroxyapatite three-dimensional self-supporting film.
Beneficial effects of the present invention are as follows：
1, the present invention coats one layer of lysozyme nanometer based on lysozyme phase transition using micro-nano granules as template on its surface
Film directly removes template and can be obtained lysozyme three-dimensional self-supporting film.The present invention can also be by electroless deposition processes in bacteriolyze
The micro-nano granules surface of enzyme cladding deposits one layer of metallic or coated in lysozyme by biomineralization micro-nano
Grain surface grows one layer of hydroxyapatite, then removes template, you can obtains metal three-dimensional self-supporting film or hydroxyapatite three
Tie up self-supporting film.
2, preparation process of the present invention is of short duration and simple easily operated while of low cost, environmentally protective, and can be used for preparing has
Machine class, inorganic and metal class three-dimensional self-supporting film have extensive universality, easily realize large-scale industrial application, and institute
Obtaining size, the shape of three-dimensional self-supporting film can be regulated and controled by template.
Description of the drawings
Fig. 1 is the optical microscopy map for the lysozyme three-dimensional self-supporting film that embodiment 1 obtains.
Fig. 2 is the scanning electron microscopy of the polystyrene microsphere for the surface deposition silver particles that embodiment 2 obtains.
Fig. 3 is the scanning electron microscopy for the three-dimensional self-supporting film of silver that embodiment 2 obtains.
Fig. 4 is the energy spectrum diagram for the three-dimensional self-supporting film of silver that embodiment 2 obtains.
Fig. 5 is the scanning electron microscopy of the polystyrene microsphere for the copper-depositing on surface particle that embodiment 3 obtains.
Fig. 6 is the scanning electron microscopy for the copper three-dimensional self-supporting film that embodiment 3 obtains.
Fig. 7 is the energy spectrum diagram for the copper three-dimensional self-supporting film that embodiment 3 obtains.
Fig. 8 is the scanning electron microscopy for the hydroxyapatite three-dimensional self-supporting film that embodiment 4 obtains.
Specific implementation mode
The present invention is described in more detail with reference to the accompanying drawings and examples, but protection scope of the present invention is not limited only to
These embodiments.
Embodiment 1
1、CaCO3The modification of particle
The 4- hydroxyethyl piperazineethanesulfonic acid buffer solutions of 10mL 5mmol/L tri- (2- carboxyethyls) phosphine are adjusted to NaOH
PH value is 7.0, is then uniformly mixed it with the 4- hydroxyethyl piperazineethanesulfonic acid buffer solutions of 10mL 1mg/mL lysozymes, then
The CaCO for being 10 μm by grain size3Particle is immersed in gained mixed liquor, is cultivated 50 minutes at room temperature, is made CaCO3Particle surface is formed
One layer of lysozyme nano thin-film obtains the CaCO of lysozyme cladding finally by centrifugation, deionized water washing3Particle.
2, lysozyme three-dimensional self-supporting film is prepared
The CaCO that lysozyme is coated3Particle is immersed in 0.1mol/L disodium ethylene diamine tetra-acetic acid aqueous solutions, at room temperature
It cultivates 60 minutes, obtains lysozyme three-dimensional self-supporting film, as shown in Figure 1.
Embodiment 2
1, the modification of polystyrene microsphere
The 4- hydroxyethyl piperazineethanesulfonic acid buffer solutions of 10mL 50mmol/L tri- (2- carboxyethyls) phosphine are adjusted to NaOH
PH value is 5.0, is then uniformly mixed it with the 4- hydroxyethyl piperazineethanesulfonic acid buffer solutions of 10mL 2mg/mL lysozymes, then
The polystyrene microsphere that grain size is 10 μm is immersed in gained mixed liquor, cultivates 40 minutes at room temperature, makes polystyrene microsphere
Surface forms one layer of lysozyme nano thin-film, finally by centrifugation, deionized water washing, obtains the polystyrene of lysozyme cladding
Microballoon.
2, the three-dimensional self-supporting film of silver is prepared
The polystyrene microsphere that lysozyme coats is immersed in 0.007g/mL tetrachloro-palladium acid aqueous ammoniums, is trained at room temperature
It educates 15 minutes, centrifugation, deionized water washing obtain the polystyrene microsphere of area load palladium ion；It is 1% by mass fraction
Silver ammino solution is uniformly mixed in equal volume with the aqueous sodium potassium tartrate that mass fraction is 4%, then by area load palladium ion
Polystyrene microsphere submergence wherein, at room temperature cultivate 15 minutes, centrifugation, deionized water washing, obtain surface deposition silver granuel
The polystyrene microsphere of son, as shown in Figure 2.The polystyrene microsphere that surface is deposited to silver particles is placed in Muffle furnace, 350 DEG C of roastings
It burns 1 hour, removes polystyrene microsphere, obtain silver-colored three-dimensional self-supporting film, as shown in Figure 3.The power spectrum of Fig. 4 further illustrates
The three-dimensional self-supporting film of fine silver is arrived.
Embodiment 3
1, the modification of polystyrene microsphere
The 4- hydroxyethyl piperazineethanesulfonic acid buffer solutions of 10mL 10mmol/L tri- (2- carboxyethyls) phosphine are adjusted to NaOH
PH value is 6.0, is then uniformly mixed it with the 4- hydroxyethyl piperazineethanesulfonic acid buffer solutions of 10mL 2mg/mL lysozymes, then
The polystyrene microsphere that grain size is 10 μm is immersed in gained mixed liquor, cultivates 40 minutes at room temperature, makes polystyrene microsphere
Surface forms one layer of lysozyme nano thin-film, finally by centrifugation, deionized water washing, obtains the polystyrene of lysozyme cladding
Microballoon.
2, copper three-dimensional self-supporting film is prepared
The polystyrene microsphere that lysozyme coats is immersed in 0.007g/mL tetrachloro-palladium acid aqueous ammoniums, is trained at room temperature
It educates 15 minutes, centrifugation, deionized water washing obtain the polystyrene microsphere of area load palladium ion；12g/L hydroxides will be contained
Sodium, 13g/L Salzburg vitriols, 29g/L sodium potassium tartrate tetrahydrates aqueous solution be uniformly mixed in equal volume with 10mL/L formalins,
Then wherein by the submergence of the polystyrene microsphere of area load palladium ion, cultivate 15 minutes at room temperature, centrifugation, deionization washing
It washs, obtains the polystyrene microsphere of copper-depositing on surface particle, as shown in Figure 5.By the polystyrene microsphere of copper-depositing on surface particle
It is placed in Muffle furnace, 350 DEG C roast 1 hour, remove polystyrene microsphere, obtain copper three-dimensional self-supporting film, as shown in Figure 6.
The power spectrum of Fig. 7 further illustrates to have obtained the three-dimensional self-supporting film of fine copper.
Embodiment 4
1, the modification of polystyrene microsphere
The 4- hydroxyethyl piperazineethanesulfonic acid buffer solutions of 10mL 1mmol/L tri- (2- carboxyethyls) phosphine are adjusted to NaOH
PH value is 7.0, is then uniformly mixed it with the 4- hydroxyethyl piperazineethanesulfonic acid buffer solutions of 10mL 1mg/mL lysozymes, then
The polystyrene microsphere that grain size is 10 μm is immersed in gained mixed liquor, cultivates 40 minutes at room temperature, makes polystyrene microsphere
Surface forms one layer of lysozyme nano thin-film, finally by centrifugation, washing, obtains the polystyrene microsphere of lysozyme cladding.
2, three-dimensional self-supporting film is prepared
The polystyrene microsphere that lysozyme coats is immersed in 20mmol/L CaCl2In aqueous solution, impregnate 2 days at room temperature,
Centrifugation, deionized water washing, remove the Ca of non-chelating2+, obtain chelating Ca2+Polystyrene microsphere；By chelating Ca2+Polyphenyl
Ethylene microballoon immerses in simulated body fluid, is kept for 10 days at 37 DEG C, obtains the polystyrene microsphere of Coated With Hydroxyapatite；By hydroxyl
The polystyrene microsphere of base apatite cladding is placed in Muffle furnace, and 350 DEG C roast 1 hour, remove polystyrene microsphere, obtain hydroxyl
Base apatite three-dimensional self-supporting film, as shown in Figure 8.
Claims (5)
1. a kind of method preparing three-dimensional self-supporting film based on lysozyme nano thin-film, it is characterised in that this method is by following steps
Rapid composition：
(1) modification of micro-nano granules
The 4- hydroxyethyl piperazineethanesulfonic acid buffer solutions of 1~100mmol/L tri- (2- carboxyethyls) phosphine are adjusted to pH value with NaOH
It is 5.0~10.0, is then uniformly mixed it with the 4- hydroxyethyl piperazineethanesulfonic acid buffer solutions of 1~50mg/mL lysozymes, then
Micro-nano granules are immersed in gained mixed liquor, are cultivated 20~60 minutes at room temperature, micro-nano granules surface is made to form one layer
Lysozyme nano thin-film, centrifugation, washing, obtains the micro-nano granules of lysozyme cladding；
(2) three-dimensional self-supporting film is prepared
Micro-nano granules inside the micro-nano granules of lysozyme cladding are removed, lysozyme three-dimensional self-supporting film is obtained；
Or by electroless deposition processes after micro-nano granules surface one layer of metallic of deposition that lysozyme coats, then remove it
Internal micro-nano granules, obtain metal three-dimensional self-supporting film；
Or by biomineralization after one layer of hydroxyapatite of micro-nano granules surface growth that lysozyme coats, then remove it
Internal micro-nano granules, obtain hydroxyapatite three-dimensional self-supporting film.
2. the method according to claim 1 for preparing three-dimensional self-supporting film based on lysozyme nano thin-film, feature exist
In：The micro-nano granules are CaCO3When particle, in step (2), the micro-nano granules that lysozyme coats are immersed in
It in 0.01~1mol/L disodium ethylene diamine tetra-acetic acid aqueous solutions, cultivates 30~90 minutes at room temperature, obtains lysozyme three-dimensional from branch
Support film.
3. the method according to claim 1 for preparing three-dimensional self-supporting film based on lysozyme nano thin-film, feature exist
In：When the micro-nano granules are polystyrene microsphere, in step (2), by the micro-nano granules surface of lysozyme cladding
After loading palladium ion, then by electroless deposition processes one layer of metallic of deposition, then roasting removes micro-nano granules, obtains metal
Three-dimensional self-supporting film.
4. the method according to claim 3 for preparing three-dimensional self-supporting film based on lysozyme nano thin-film, feature exist
In：The metal is copper or silver.
5. the method according to claim 1 for preparing three-dimensional self-supporting film based on lysozyme nano thin-film, feature exist
In：When the micro-nano granules are polystyrene microsphere, in step (2), by the micro-nano granules surface of lysozyme cladding
Chelating Ca2+Afterwards, it immerses in simulated body fluid, is kept at 37 DEG C 7~10 days, then roasting removes micro-nano granules, obtains hydroxyl
Apatite three-dimensional self-supporting film.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810587315.9A CN108751126B (en) | 2018-06-08 | 2018-06-08 | The method for preparing three-dimensional self-supporting film based on lysozyme nano thin-film |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810587315.9A CN108751126B (en) | 2018-06-08 | 2018-06-08 | The method for preparing three-dimensional self-supporting film based on lysozyme nano thin-film |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108751126A true CN108751126A (en) | 2018-11-06 |
| CN108751126B CN108751126B (en) | 2019-10-25 |
Family
ID=63999683
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810587315.9A Active CN108751126B (en) | 2018-06-08 | 2018-06-08 | The method for preparing three-dimensional self-supporting film based on lysozyme nano thin-film |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108751126B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110028586A (en) * | 2019-04-24 | 2019-07-19 | 陕西师范大学 | A kind of large biological molecule microreactor fixed based on protein film isolation |
| CN115365494A (en) * | 2022-09-13 | 2022-11-22 | 嘉兴学院 | Preparation method of silver-coated copper powder and application of silver-coated copper powder in conductive paste |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101966441A (en) * | 2010-09-09 | 2011-02-09 | 天津大学 | Polydopamine microcapsules and preparation method thereof |
| CN102430377A (en) * | 2011-09-28 | 2012-05-02 | 浙江大学 | Method for preparing hollow microcapsules with micron sized walls by inducing templates |
| CN104003404A (en) * | 2014-05-19 | 2014-08-27 | 国家纳米科学中心 | Preparation method and application of porous silicon dioxide nano particle |
| CN105153443A (en) * | 2015-08-27 | 2015-12-16 | 陕西师范大学 | Biological protein two-dimensional nano-film prepared by using lysozyme and preparation method of biological protein two-dimensional nano-film |
| CN105296977A (en) * | 2015-10-29 | 2016-02-03 | 陕西师范大学 | Method for performing metal electroless deposition based on lysozyme two-dimension nano-film |
| CN105418959A (en) * | 2015-12-09 | 2016-03-23 | 天津大学 | Preparation method of protamine-silicon oxide porous hybridization micro-capsules |
| CN105861478A (en) * | 2016-04-19 | 2016-08-17 | 青岛农业大学 | Nanometer chitin-lysozyme composite particles and preparation method thereof |
| US20170341077A1 (en) * | 2016-05-31 | 2017-11-30 | University Of Guelph | Microfluidic biosensor for allergen detection |
-
2018
- 2018-06-08 CN CN201810587315.9A patent/CN108751126B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101966441A (en) * | 2010-09-09 | 2011-02-09 | 天津大学 | Polydopamine microcapsules and preparation method thereof |
| CN102430377A (en) * | 2011-09-28 | 2012-05-02 | 浙江大学 | Method for preparing hollow microcapsules with micron sized walls by inducing templates |
| CN104003404A (en) * | 2014-05-19 | 2014-08-27 | 国家纳米科学中心 | Preparation method and application of porous silicon dioxide nano particle |
| CN105153443A (en) * | 2015-08-27 | 2015-12-16 | 陕西师范大学 | Biological protein two-dimensional nano-film prepared by using lysozyme and preparation method of biological protein two-dimensional nano-film |
| CN105296977A (en) * | 2015-10-29 | 2016-02-03 | 陕西师范大学 | Method for performing metal electroless deposition based on lysozyme two-dimension nano-film |
| CN105418959A (en) * | 2015-12-09 | 2016-03-23 | 天津大学 | Preparation method of protamine-silicon oxide porous hybridization micro-capsules |
| CN105861478A (en) * | 2016-04-19 | 2016-08-17 | 青岛农业大学 | Nanometer chitin-lysozyme composite particles and preparation method thereof |
| US20170341077A1 (en) * | 2016-05-31 | 2017-11-30 | University Of Guelph | Microfluidic biosensor for allergen detection |
Non-Patent Citations (1)
| Title |
|---|
| YAJUN WANG ET AL.: ""Template Synthesis of Stimuli-Responsive Nanoporous Polymer-Based Spheres via Sequential Assembly"", 《CHEMISTRY OF MATERIALS》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110028586A (en) * | 2019-04-24 | 2019-07-19 | 陕西师范大学 | A kind of large biological molecule microreactor fixed based on protein film isolation |
| CN115365494A (en) * | 2022-09-13 | 2022-11-22 | 嘉兴学院 | Preparation method of silver-coated copper powder and application of silver-coated copper powder in conductive paste |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108751126B (en) | 2019-10-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Wei et al. | Preparation of hierarchical hollow CaCO3 particles and the application as anticancer drug carrier | |
| Yang et al. | Biomimetic encapsulation of individual cells with silica | |
| Fakhrullin et al. | “Face-lifting” and “make-up” for microorganisms: layer-by-layer polyelectrolyte nanocoating | |
| Dierendonck et al. | Just spray it–LbL assembly enters a new age | |
| Wang et al. | Yeast cells with an artificial mineral shell: protection and modification of living cells by biomimetic mineralization | |
| CN108751126B (en) | The method for preparing three-dimensional self-supporting film based on lysozyme nano thin-film | |
| Yang et al. | Cytocompatible encapsulation of individual Chlorella cells within titanium dioxide shells by a designed catalytic peptide | |
| CN100594186C (en) | Chitose aquagel evoked original position synthesis of super-paramagnetism nano ferriferrous oxide particles | |
| Hu et al. | Magnetically Actuated Rolling of Star‐Shaped Hydrogel Microswimmer | |
| Balkundi et al. | Encapsulation of bacterial spores in nanoorganized polyelectrolyte shells | |
| Shi et al. | Preparation and enzymatic application of flower-like hybrid microcapsules through a biomimetic mineralization approach | |
| CN107789332B (en) | Calcium carbonate/calcium alginate composite microspheres capable of adjusting drug release rate and prepared based on double-aqueous-phase biomineralization technology | |
| Lei et al. | Bioinspired cell silicification: from extracellular to intracellular | |
| GB2427157A (en) | Coated microspheres | |
| Hou et al. | Formulation of robust organic–inorganic hybrid magnetic microcapsules through hard-template mediated method for efficient enzyme immobilization | |
| Zhang et al. | Metal-phenolic network coatings for engineering bioactive interfaces | |
| Su et al. | In situ self‐assembly of coacervate microdroplets into viable artificial cell wall with heritability | |
| US20110177231A1 (en) | Nano-, Micro-, Macro- Encapsulation And Release Of Materials | |
| Wang et al. | Cellular shellization: Surface engineering gives cells an exterior | |
| Shi et al. | Synthesis of organic–inorganic hybrid microcapsules through in situ generation of an inorganic layer on an adhesive layer with mineralization-inducing capability | |
| CN108096586A (en) | Double-bang firecracker based on manganese dioxide modification answers preparation method, product and the application of drug delivery system | |
| Feng et al. | Extracellular silica nanocoat formed by layer-by-layer (LBL) self-assembly confers aluminum resistance in root border cells of pea (Pisum sativum) | |
| CN109010318A (en) | A kind of preparation method of the amido modified 3-d tree-like silica dioxide nano particle carrier for carrying hydrophilic small molecule drugs PH response | |
| Tian et al. | Nuclease-responsive DNA–PEI hollow microcapsules for bio-stimuli controlled release | |
| Hong et al. | Cationic polymers for coating living cells |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |