CN1087494A - The animal by autolysis leftover bits and pieces prepares the method for nutritious drink - Google Patents
The animal by autolysis leftover bits and pieces prepares the method for nutritious drink Download PDFInfo
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- CN1087494A CN1087494A CN93109935A CN93109935A CN1087494A CN 1087494 A CN1087494 A CN 1087494A CN 93109935 A CN93109935 A CN 93109935A CN 93109935 A CN93109935 A CN 93109935A CN 1087494 A CN1087494 A CN 1087494A
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Abstract
A kind of be used for tankage carried out whole self-dissolving after, preparation is for the method for the human nutritious drink of taking.With the macromolecule organic matter that is contained in the tankage (liver, lung, spleen, pancreas, myelencephalon, eye, blood, hoof first, waste wool are sent out), carry out whole self-dissolving cooperate again add enzyme hydrolysis after, the low molecular organic material that resolves into nature is made nutritious drink.It is the enzyme source with the hydrolase numerous in variety that is contained in liver, spleen, the leucocyte, by selecting composition of raw materials, sanitary standard, pH value scope and reflection time; Through roughing, self-dissolving and the hydrotropy of borrow and storage, raw material, concentrate, dry, seven technical process such as seal up for safekeeping, preparing nutrient enriches, the beverage of health properties uniqueness.
Description
A kind of be used for the animal slaughtering leftover bits and pieces carried out whole self-dissolving after, preparation is for the method for the human nutritious drink of taking.
General tankage is used as fodder mostly, is utilized by the people after transforming by animal body again.Except that indivedual tissues, organ such as minorities such as blood, liver were directly eaten by the mankind, serum can be processed to protein food or beverage; Red blood cell, myelencephalon, lung, spleen, pancreas, eye, hoof first, waste wool such as send out at the raw material that is used to biochemical pharmacy on a small quantity, and the overwhelming majority goes out of use or is used as the extremely low feed of conversion ratio.Reason is their low quality, and proterties is poor, and digestive utilization ratio is low.Next is to lack processing method preferably.
At present, people extensively utilize strong acid, highly basic, pressurizing hydrolysis both at home and abroad or add methods such as enzyme hydrolysis method, fermentation method to prepare feed or medical product except that the nutrition that these leftover bits and pieces contained having been done analysis fully.But they are not that technology is coarse, do not reach human edible sanitary standard, are exactly that complex process, production scale are restricted, cost is high, the cycle is long, nutrient destroys seriously, are not suitable for the production oral food.Though some units also had tissue automatic soup-dissolving's Study on Technology of indivedual internal organs to use, as the production technology of nucleic acid-amino acid sheet, (Zhang Tianming, Wu Wutong, Wang Youtong, Lu Dezhang chief editor animal biochemical pharmacy are learned Beijing people's health publishing house 1,983 202) of utilizing pancreas homogenate autolysis method to realize exactly.But also be not used for the whole self-dissolving and the food processing of animal slaughtering leftover bits and pieces.
The objective of the invention is liver with slaughtered animals, spleen, pancreas, lung, myelencephalon, eye, blood, the hoof first, waste wool such as sends out at the integral body of histoorgan as leftover bits and pieces, ratio in science is arranged in pairs or groups, utilize their self-contained hydrolases to carry out cooperating again after the part self-dissolving and add enzyme hydrolysis, macromolecule organic matter is decomposed into the lower-molecular substance of nature, the preparation nutritious drink, both helped changing their proterties, quality, improve digestive utilization ratio, enlarge human food's resource, exploitation food new varieties, help developing the new purposes of leftover bits and pieces again, reduce the pollution of animal slaughtering processing environment.
The present invention is with the proteolytic enzyme numerous in variety that is contained in liver, spleen, pancreas, the leucocyte (trypsase, cathepsin and lysosome composite hydrolytic enzyme), as the enzyme source of the whole self-dissolving of leftover bits and pieces; By the prescription of choose reasonable raw material, pH value scope, reaction temperature and reaction time.Through roughing, self-dissolving and the hydrotropy of borrow and storage, raw material, filtration, concentrated, drying, seal etc. that seven steps realize up for safekeeping.Concrete grammar is as follows:
One, borrow and storage
(1) liver, spleen, pancreas, myelencephalon, eye, lung are avoided the pollution of bacterium, mycotic spore and impurity by the conventional method collection of butchering processing, and the relevant rules that sanitary standard is formulated by unification of the motherland are carried out.General fresh processing, if need storage, can be by after the classification of internal organs kind, warehouse-in in 6～8 hours after butchering was preserved 3～15 days for-30～4 ℃.
(2) collection of blood needs to be collected in the sterility container after aseptic the taking, anti-freezing, and in 0～4 ℃ of preservation 3～15 days, even making freezed, and the holding time can add an amount of ascorbic acid or glycerine than length.
(3) collection sent out of hoof first, waste wool and storage are carried out routinely.
Two, the roughing of raw material
(1) roughing sent out of hoof first, waste wool: hoof first, hair is carcass and the extraneous organ that contact, adds and butchers inevitably pollution in the process, wherein is rich in impurity and brood cell bacterium.Its roughing process is divided into selection, washing sterilization, levigate three environment.
1, selects: promptly select to remove foreign matter and impurity.
2, washing: add liquid detergent with ordinary water earlier and carry out rinsing, go to wash 20～30 minutes with clear water behind absolute oil dirt and the dust, then with distilled water washing 2～3 times.15 pounds of autoclavings 0.5～1 hour, shine (baking) dried.
3, levigate: hair, the hoof first of oven dry is levigate with flour mill earlier, being as the criterion by 20～40 mesh sieves.
(2) roughing of eye, lung: eye, the organ of lung for communicating with the external world, include multiple bacterial parasite, their roughing is divided into washing, three links of smashing to pieces, sterilize.
1, washing: lung removes tracheae earlier.With ordinary water flush away impurity, use distilled water flushing then 2～3 times.
2, smash to pieces: smash into homogenate with high-speed tissue mashing machine.
3, sterilization: use the clean container splendid attire, the steam boiling 60 minutes of flowing.Also available autoclaving.
(3) roughing of blood: mainly be to remove ferroheme.Be divided into separation, solidify, decolour, wash four processes.
1, separate: exactly red blood cell is separated, white blood cell and serum directly use.Centrifugal process commonly used was promptly used horizontal electric centrifuge 1000G centrifugal 60 minutes.If no centrifuge can directly be used to blood clot solidify, decolour, serum directly utilizes, also not anti-freezing during collection.
2, solidify: red blood cell or blood clot are carried out boiling, the complete sex change of protein is solidified.
3, decolour: the hemoglobin that will solidify is done suitably dilution (being as the criterion stirring) with distilled water in reactor, with hydrochloric acid adjust pH to 1～2, and room temperature or the aquation of stirring below 40 ℃ 30 minutes, all cloth filters.3～4 times so repeatedly, till the coagulated protein filter residue is canescence.Filtrate being used to produced ferroheme.
4, washing: the hemoglobin that solidifies after will decolour earlier washes to filtrate closely neutrally with clear water, wash with distilled water and get final product for 2～3 times.
(4) liver, spleen, pancreas, encephalomyelic roughing: before the self-dissolving liquefaction reaction, respectively in proportion.Smash into homogenate with high-speed tissue mashing machine.Pancreas homogenate needs other splendid attire.
(5) if other leftover bits and pieces such as use cartilage can be cleaned it, wear into homogenate after the sterilization and get final product.
Three, self-dissolving and hydrotropy:
(1) composition of raw materials:
The total principle of composition of raw materials is, should improve the overall value of finished product, makes each nutrient of finished product more near the needs of human body, embodies the integrality of utilizing tankage again.That is: liver 1～95%, spleen 1～95%, and pancreas 1～50%, hemoglobin and serum 1～95%, lung 1～95%, myelencephalon 1～20%, eye 0～5%, hoof first, waste wool sends out 1～50%, and other is 1～95% years old.
Preferably use the natural proportion that each composition of leftover bits and pieces produces in the animal slaughtering process.Reference formulation is as follows: liver 28.94%, and spleen 3.65%, pancreas 2.48%, blood 45.96%, lung 11%, myelencephalon 2.06%, eye 0.5%, hoof first, waste wool sends out 5.36%.
(2) thinner ratio:
The selection of dilution ratio directly influences the self-dissolving effect, press total raw material (fresh state) and calculate, with 1: 1～20(V/V) be as the criterion.Consider the dual relationships of the molten long-pending and self-dissolving effect of reaction, preferably select 1: 5～10.
(3) self-dissolving:
Earlier by the dilution ratio of volume that adds raw material (also can weight calculate) and selection, the distilled water that should add adds in the reactor (enamel), and heat temperature raising to 45 ℃ is again with liver, spleen homogenate adds, and adds 1/2500(simultaneously and calculates with the reactant liquor cumulative volume) chloroform.Behind the solubilizing reaction 1～5 minute, add other raw material (the pancreas slurry does not add) again.30～50 ℃ of constant temperature stirring reactions 2～4 hours.Add pancreas homogenate, with milk of lime or Na2CO3(AR) adjust pH to 6.8～7.6, add 1/2500 chloroform again, 30～50 ℃ of constant temperature stirring reactions 2～4 hours.The total time of self-dissolving reaction is 4～8 hours.
(4) hydrotropy: ammonia nitrogen accounts for the ratio of total nitrogen in the product in order to improve, so hydrotropy is set, method is:
Based on aforementioned self-dissolving reactant liquor, with milk of lime or Na2CO3(AR) adjust pH to 6.8～7.6, the chloroform of adding 1/2500 is pressed 0.3% of material quantity again and is added 3 of trypsase or 17/5 unit, 942 enzymes (unit: ten thousand units per liter), 30～50 ℃ of constant temperature stirring reactions 2～4 hours.Hydrotropy is 1～2 time so repeatedly, with do not contain the coagulated protein filter residue in the reactant liquor, solution is transparent or semitransparent state and is as the criterion.Reaction total time is 6～16 hours.Reaction temperature preferably is controlled between 40～45 ℃.
Four, filter
The reactant liquor finished filtered with white all cloth or with white silk suction filtration carefully.And then add 1/2500 chloroform, with hydrochloric acid or Na2CO3 adjust pH to 5～6 of 6N.
Five, concentrate
Reactant liquor after filtering is moved in the vacuum concentrator, and 20～50 ℃ are condensed into paste.Press the heavy multitudinous sugar of 10～30% addings of raw material, shift out inspissator.
Six, drying
Under 20～50 ℃ of conditions, concentrate is carried out vacuum drying, make into powdered.Also can use freeze drying or spray-drying.
Seven, seal up for safekeeping
Dried powdered product is carried out the vacuum packaging back with lighttight packaging bag by specification to be preserved.
Nutrition is analyzed
Quantitative composition: (in 100g) carbohydrate 20～60g, lipid 3～5g, total amino acid content 15～45g; Wherein: lysine 0.3～0.9g, tryptophan 0.8～2g, methionine 0.2～0.55g, cystine 0.3～0.9g, threonine 0.25～0.6g, isoleucine 0.7～2g, histidine 2.5～6g, valine 0.6～1.5g, leucine 1.6～3.5g, arginine 0.6～1.5g, phenylalanine 0.6～1.5g, glycine 0.18～4g, cholesterol 50～200mg, calcium 15～30mg, phosphorus 150～250mg, vitamin A 4000～5000iu, Cobastab
12350～500 μ g, Cobastab
10.1～0.25mg, vitamin B2 0.5～1.5mg, nicotinic acid 0.5～1mg, pantothenic acid 3.5～5mg, choline 1.5～30mg, iron 10～20mg, copper 0.5～0.9mg, manganese 0.2～0.28mg, zinc 1～1.5mg.
Qualitative composition: aspartic acid, glutamic acid, tyrosine, serine, proline, alanine, mucopolysaccharide, cytidylic acid, AC, GC, GGC, GAC, GGU waits dinucleotides and trinucleotide.(A-adenylic acid, G-guanylic acid, C-cytidylic acid, U-uridylate).
Function: learn principle according to the animal biochemical pharmacy, the raw materials used and technical process in conjunction with the present invention, can predict that the nutritious drink that the present invention produces may have following function:
(1) supplement the nutrients, prevention is malnutritive; Promote baby and children's normal development.
(2) anti-pernicious anaemia and megaloblastic anemia.
(3) increase white blood cell, suppress the growth of cancer cell, initial stage liver cancer and cancer chemotherapy are had the supplemental treatment effect.
(4) angina pectoris, cardiac insufficiency, rheumatic arthritis, eclampsia, psoriasis, chronic hepatitis, encephalitis, bronchial astehma, allergic rhinitis and tracheitis there is the supplemental treatment effect.
(5) separate the ammonia poison in prevention fatty degeneration of liver and cirrhosis, the acceleration bodies; The treatment digestive tract ulcer, the acidity of regulating gastric juice.
The present invention compares with existing food-processing method, and following good effect is arranged: (1) technology is simple, and cost is low, is convenient to food_beverage industry and goes into operation.(2) be that integral body is carried out self-dissolving with the leftover bits and pieces, both changed the quality that can't eat, fully preserved the nutrient that they contain itself again as leftover bits and pieces.(3) various raw materials are arranged in pairs or groups in the ratio of science, have both helped nutrition complement, have intactly utilized the leftover bits and pieces of animal slaughtering processing again.(4) enlarge food resource, developed the human foods new varieties, reduced environmental pollution.
Technical process example (composition of raw materials is by natural proportion).
Thinner ratio (V/V) reaction temperature (℃) reaction time (S) liquefaction effect
1:5 40 8 aubergines are translucent, residue 2%
1:10 40 6 aubergines are translucent, residue 1.5%
1:10 45 6 aubergines are translucent, residue 1.4%
1:10 50 6 aubergines are translucent, residue 2.5%
1:15 45 6 aubergines are translucent, residue 1.5%
1:20 45 6 aubergines are translucent, residue 1.8%
1:25 45 6 aubergines are translucent, residue 2%
Residue is put the heavy ratio of whole raw material for the filter residue weight in wet base
Claims (2)
1, a kind of method that is used for tankage is carried out preparing after the whole self-dissolving nutritious drink, it is characterized in that: used raw material is the integral body of slaughtered animals leftover bits and pieces, is the enzyme source with the composite hydrolysis enzyme that is contained in the tankage (liver, spleen, pancreas, leucocyte); By choose reasonable composition of raw materials, sanitary standard, pH value scope and reflection time; Through borrow and storage, the roughing of raw material, self-dissolving and hydrotropy, concentrate, dry, technical process such as seal up for safekeeping, the preparation nutrient is abundant, health properties uniqueness, the nutritious drink of taking for the mankind.
2, according to claim: the integral body of (1) described leftover bits and pieces is characterized in that: comprised that myelencephalon, lung, eye, spleen, liver, pancreas, hoof first, waste wool internal organs and blood tissues such as send out.(2) described composition of raw materials, it is characterized in that: the weight with the fresh natural state is calculated, liver, spleen, pancreas, leucocyte etc. contain enzyme to organize ratio are 5～80%, wherein: liver 1～95%, spleen 1～95%, pancreas 1～50%, hemoglobin and serum 1～95%, lung 1～95%, myelencephalon 1～20%, eye 0～5%, hoof first, waste wool sends out 1～50%.(3) the described reaction time, it is characterized in that 6～16 hours.(4) the composite hydrolysis enzyme system that itself is contained in the described tankage is characterized in that: be mainly the cathepsin and the lysosome composite hydrolytic enzyme that are contained in liver, spleen, pancreas, the white blood cell.(5) described reaction temperature is characterized in that: 30～50 ℃.(6) described pH value scope is characterized in that 6.8～7.6.(7) described nutrient is abundant, it is characterized in that: quantitative composition: (in 100g) carbohydrate 20～60g, lipid 3～5g, total amino acid content 15～45g; Wherein: lysine 0.3～0.9g, tryptophan 0.8～2g, methionine 0.2～0.55g, cystine 0.3～0.9g, threonine 0.25～0.6g, isoleucine 0.7～2g, histidine 2.5～6g, valine 0.6～1.5g, leucine 1.6～3.5g, arginine 0.6～1.5g, phenylalanine 0.6～1.5g, glycine 0.18～4g, cholesterol 50～200mg, calcium 15～30mg, phosphorus 150～250mg, vitamin A 4000～5000iu, Cobastab
12350～500 μ g, Cobastab
10.1～0.25mg, Cobastab
20.5～1.5mg, nicotinic acid 0.5～1mg, pantothenic acid 3.5～5mg, choline 1.5～30mg, iron 10～20mg, copper 0.5～0.9mg, manganese 0.2～0.28mg, zinc 1～1.5mg.Qualitative composition has: aspartic acid, glutamic acid, tyrosine, serine, proline, alanine, mucopolysaccharide, cytidylic acid, AC, GC, GGC, GAC, GGU.(8) described health properties uniqueness is characterized in that: have extra-nutrition, prevention is malnutritive, promotes baby and children's normal development.Anti-pernicious anaemia and megaloblastic anemia.Increase white blood cell, suppress cancer cell growth, initial stage liver cancer and cancer chemotherapy are had the supplemental treatment effect.Angina pectoris, cardiac insufficiency, rheumatic arthritis, eclampsia, psoriasis, chronic hepatitis, encephalitis, bronchial astehma, allergic rhinitis and tracheitis there is the supplemental treatment effect.Separate the ammonia poison in prevention fatty degeneration of liver and cirrhosis, the acceleration bodies; The treatment digestive tract ulcer, the acidity of regulating gastric juice.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN93109935A CN1087494A (en) | 1993-08-13 | 1993-08-13 | The animal by autolysis leftover bits and pieces prepares the method for nutritious drink |
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| CN93109935A CN1087494A (en) | 1993-08-13 | 1993-08-13 | The animal by autolysis leftover bits and pieces prepares the method for nutritious drink |
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| CN1087494A true CN1087494A (en) | 1994-06-08 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100346726C (en) * | 2003-03-25 | 2007-11-07 | 于靖波 | Bioactive drink and its preparing method |
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1993
- 1993-08-13 CN CN93109935A patent/CN1087494A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100346726C (en) * | 2003-03-25 | 2007-11-07 | 于靖波 | Bioactive drink and its preparing method |
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