CN108743656A - Kuh-seng Radix Glycyrrhizae prescription for treating non-alcohol fatty liver - Google Patents
Kuh-seng Radix Glycyrrhizae prescription for treating non-alcohol fatty liver Download PDFInfo
- Publication number
- CN108743656A CN108743656A CN201811083160.1A CN201811083160A CN108743656A CN 108743656 A CN108743656 A CN 108743656A CN 201811083160 A CN201811083160 A CN 201811083160A CN 108743656 A CN108743656 A CN 108743656A
- Authority
- CN
- China
- Prior art keywords
- kuh
- seng
- radix glycyrrhizae
- liver
- group
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/489—Sophora, e.g. necklacepod or mamani
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/484—Glycyrrhiza (licorice)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- Biotechnology (AREA)
- Mycology (AREA)
- Epidemiology (AREA)
- Microbiology (AREA)
- Medical Informatics (AREA)
- Botany (AREA)
- Alternative & Traditional Medicine (AREA)
- Gastroenterology & Hepatology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention belongs to liver disease fields, and in particular to the kuh-seng Radix Glycyrrhizae prescription for treating non-alcohol fatty liver.Kuh-seng and the component proportion of Radix Glycyrrhizae are：1：0.1~5.《Kuh-seng Radix Glycyrrhizae prescription》Meet six basic demands of the modernization of Chinese medicine：1. curative for effect, adverse reaction is small；2. meeting the composition principle of traditional Chinese medicine, it is derived from the new treatment function of classics recipe；3. crude drug source is abundant, meet " non-rarity " principle；4. pharmacological action can be proved by modern experimental pharmacological method；5. independent intellectual property can be formed；6. prescription is simple, and has specific quality control standard.
Description
Technical field
The invention belongs to liver disease fields, and in particular to the kuh-seng Radix Glycyrrhizae group for treating non-alcohol fatty liver
Side.
Background technology
Non-alcohol fatty liver is (caused by referring in addition to alcohol and other specific damage liver factors, with diffusivity liver
Cell Macrovesicular steatosis be main feature clinical pathology syndrome, including simple fatty liver (and by its differentiation fat
Fat hepatitis and hepatic sclerosis), insulin resistance and genetic predisposition and its onset relation are close.World's gastroenterology can be 2014
The data of newest announcement are shown：Average adult's illness rate is 27-33%, and the illness rate of obesity patient is 75-92%, Huan Zhezao
Phase is simple fatty liver, can gradually develop into nonalcoholic fatty liver disease (NASH), clinically the patient Ke Fa of 10-20%
Exhibition is NASH, eventually leads to liver fibrosis and hepatic sclerosis.At present in China, the adult prevalence rate of NAFLD/NASH is left close to 15%
The right side, and in rising and rejuvenation trend year by year.
Non-alcoholic simple fatty liver insulin resistance, oxidative stress, the cell factor the effects that under can be evolved into it is non-
Alcoholic fatty liver inflammation, hepatic sclerosis.Effective treatment for nonalcoholic fatty liver is the key that prevent hepatic sclerosis,
It is the emphasis of recent researches.
For nonalcoholic fatty liver and its caused nonalcoholic fatty liver disease, lack effective control measure at present.
World's gastroenterology can be issued《Nonalcoholic fatty liver practice guidelines in 2014》It proposes：Clinical research shows single give
Insulin sensitizer cannot significantly improve conditions of patients, and insulin sensitizer has the side effect of hepatic and renal function damage.Closely
The statins and omega-fatty acid that year proposes, which are also not known, can be used for treating.Anti-oxidant class drug：Such as vitamin E is not yet
It is recommended to use.NAFLD pathogenesis is complicated, but chemical synthetic drug therapy target is single, and there are notable limitations, even more lacks
Weary prevention NAFLD develops into the active drug of NASH.China's non-alcohol fatty liver practice guidelines point out, diet control,
Mode of making the life better improves endotoxemia using lipid-loweringing, hepatic and insulin sensitizer, adjusts cytokine levels
The methods of may to prevent its develop and treatment have certain effect.The pathogenesis of nonalcoholic fatty liver can't be used single at present
One mechanism explains that scholar generally believes that it is a kind of and the diseases related and equal propositions of genotypic environment metabolic stress factor
" two-hit " theory is a theory being most widely recognized as.This theory proposes：Insulin resistance and disorders of lipid metabolism
Caused liver cell fat generation and steatosis make liver stimulate inflammatory reaction and various damages as first strike
Sensibility increases.When the factors production such as oxidative stress and lipid peroxidation, the release of inflammatory cytokine, mitochondrial function exception
At then foring two-hit, this reacts induction of liver inflammation, degeneration of liver cells necrosis and liver fibrosis even hepatic sclerosis
Occur.
At present Chinese prescription can be used for NAFLD or NASH treatment mainly have Kezhi capsule, silybum mariamum and herba swertiae contained liver-benefiting, strong liver glue
Capsule changes the treatment that the Chinese patent drugs such as stagnant Rougan Granule, fatty liver treating notoginseng ball apply to clinical nonalcoholic fatty liver.But above group at present
Square common problem is：1. big prescription, complicated component, Quality Control are difficult to control；2. treatment has limitation, it is mainly used for
The generation of NAFLD patient's fatty lesion or steatohepatitis, cannot effectively both treatments simultaneously；3. prescription is complicated, it is difficult to use
Modern pharmacology theory illustrates active ingredient.
Kuh-seng Radix Glycyrrhizae prescription is in Ming Dynasty's medical book《Many wonderful celestial being sides》With regard on the books, the party only has kuh-seng, two taste of Radix Glycyrrhizae.《A thousand pieces of gold
It will side》With《Prescriptions for Universal Relief》In, also all record kuh-seng glycyrrhiza decoction, kuh-seng and the main component that Radix Glycyrrhizae is kuh-seng glycyrrhiza decoction.?《Scholar
Door thing parent》In, the ZHANG Cong-zheng of one of Au element has done following description to kuh-seng Radix Glycyrrhizae prescription：" all medicines are all malicious, though it is sweet
Grass, kuh-seng, poison that can not be unexpectedly, then the five tastes respectively return its dirty to long term usage, must have the trouble of victory gas increasing partially, all medicines all right ".
Kuh-seng Radix Glycyrrhizae prescription, by kuh-seng, Radix Glycyrrhizae prescription；Kuh-seng Radix Glycyrrhizae prescription is loaded in Ming Dynasty's medical book《Many wonderful celestial being sides》, until《Thousand
Jin Fang》And《Prescriptions for Universal Relief》Also it records and there was only kuh-seng, two medicine of Radix Glycyrrhizae, the infantile malnutrition due to digestive disturbances or intestinalparasites dysentery for treating dampness stomach in kuh-seng glycyrrhiza decoction side
Incessantly.Kuh-seng, bitter cold enter the heart, Return liver, stomach, large intestine channel, function heat-clearing and damp-drying drug and control various damp and hot, phlegm it is hot caused by the insufficiencies of the spleen with overabundance of dampness,
The damp retention in middle-jiao of phlegm resistance is demonstrate,proved, and in particular for phlegm stagnation, qi depression to blood stasis, phlegm is turbid, Yu Xue Bi resistance is demonstrate,proved in the phlegm wet of liver network.
Due to its big bitter Great Cold, drop lets out that cleanse the benefits of damp and hot too high strong, need to match Radix Glycyrrhizae mediation property of medicine, and clearing heat and detoxicating.
Invention content
The purpose of the present invention is：Derived from Chinese medicine classics recipe, the hardship that can be used for treating non-alcohol fatty liver is provided
Join Radix Glycyrrhizae prescription, to overcome above-mentioned defect in the prior art, and is the secondary development of Chinese medicine classics recipe.
The adopted technical solution is that：
Kuh-seng Radix Glycyrrhizae prescription for treating non-alcohol fatty liver, it is characterised in that：The weight of kuh-seng, Radix Glycyrrhizae is matched
Than for：1：0.1~5.
Further, the weight proportion of kuh-seng, Radix Glycyrrhizae is：1：1.
Further, the composition form of the kuh-seng Radix Glycyrrhizae includes kuh-seng, traditional Chinese medicine of licorice root material or its extract or its activity
Monomer.
Further, the extract of the kuh-seng, Radix Glycyrrhizae includes water extract or alcohol extract.
Further, the activated monomer of the kuh-seng includes (matrine, the chief actives such as oxymatrine monomer), Radix Glycyrrhizae
Activated monomer includes the chemical derivative of (glycyrrhizic acid, the chief actives such as enoxolone monomer) and activated monomer.
Further, the dosage form of the kuh-seng Radix Glycyrrhizae prescription is tablet, pill, particle, capsule or injection.
The beneficial effects of the invention are as follows：《Kuh-seng Radix Glycyrrhizae prescription》Meet six basic demands of the modernization of Chinese medicine：1. curative effect
Definitely, adverse reaction is small；2. meeting the composition principle of traditional Chinese medicine；3. crude drug source is abundant, meet " non-rarity " principle；4. medicine
Reason effect can be proved by modern experimental pharmacological method；5. independent intellectual property can be formed；6. prescription is simple, and with bright
True quality control standard.
Those skilled in the art can directly be used as medicine on the basis of active constituents of medicine of the present invention obtained to be taken or is added
Required oral preparation is produced in conventional fashion into acceptable auxiliary material in pharmacy.Can such as be made common tablet (dispersible tablet,
Effervescent tablet, oral disnitegration tablet, lozenge, chewable tablets, effervescent tablet), capsule (hard capsule, soft capsule), granule, pill (drop
Pill), the oral drugs of the solid dosage forms such as powder, the liquid such as syrup, oral solution, aqua, mixture, decoction can also be made
The oral drugs of dosage form.Therefore, in the pharmaceutical composition in addition to active ingredient, pharmaceutically acceptable can also be contained
Auxiliary material.Preferably, pharmaceutical composition of the present invention according to conventional fabrication process be prepared into oral solution, aqua, electuary, capsule or
Powder etc..
Description of the drawings
Fig. 1 is that liver HE dyes comparison chart in embodiment 1.1；
Fig. 2 is liver oil red O stain comparison chart in embodiment 1.1；
Fig. 3 is that liver HE dyes comparison chart in embodiment 1.2；
Fig. 4 is liver oil red O stain comparison chart in embodiment 1.2；
Fig. 5 is that liver HE dyes comparison chart in embodiment 2.1；
Fig. 6 is liver oil red O stain comparison chart in embodiment 2.1；
Fig. 7 is that liver HE dyes comparison chart in embodiment 2.2；
Fig. 8 is liver oil red O stain comparison chart in embodiment 2.2；
Wherein, big figure X 200, small figure X 100.
Specific implementation mode
Below in conjunction with the attached drawing of the present invention, technical scheme of the present invention is clearly and completely described.Based on this hair
Embodiment in bright, the every other implementation that those of ordinary skill in the art are obtained without creative efforts
Example, shall fall within the protection scope of the present invention.
Embodiment 1 is studied《The influence for the nonalcoholic fatty liver mouse model that kuh-seng Radix Glycyrrhizae induces high lipid food》
1.1 research《The nonalcoholic fatty liver mouse model that kuh-seng Radix Glycyrrhizae prescription is induced with folk prescription comparing high lipid food
Influence》
Purpose and meaning：Study kuh-seng Radix Glycyrrhizae prescription, the influence of kuh-seng, Radix Glycyrrhizae to NAFLD mouse liver functions.
Method：C57BL/6J male mices (20-22g), are randomly divided into 5 groups, every group 10, high in fat in addition to blank group (A)
After diet modeling 4 weeks, high fat diet (is purchased from Nantong Te Luofei feed corporation,Ltds, code T P26301).Gastric infusion
(B) model group gives physiological saline gavage；(C) group gives kuh-seng Radix Glycyrrhizae prescription water decoction gavage；(D) group gives kuh-seng gavage；
(E) group is given Radix Glycyrrhizae gavage and is put to death after continuation HFD feeds and is administered 12 weeks, detects mouse liver function index of correlation.
1. kuh-seng Radix Glycyrrhizae prescription can significantly reduce model mouse weight, liver weight；
The situation of ingesting for recording mouse weekly, records the weight of mouse every 2 weeks.Experiment terminate first 1 day, to mouse take every
Curfew eats.Second day detects the empty body weight and height of mouse, and calculates Lee ' s indexes：Lee ' s indexes=weight (g) 1/
3*1000* heights (cm) -1.Vena orbitalis posterior clump blood sampling is carried out to all mouse, detaches serum.It is rapid to put to death after mouse anesthesia
And detach liver organization.Then liver index is calculated：Liver index=liver weight (g)/empty body weight (g) * 100%.Experiment need to be left and taken
Left lobe of liver tissue, and a part therein is fixed on 10% neutral formalin solution kind, remain pathological analysis and reality
It tests.
As a result：
**P<0.05 compared with blank group, * P<0.05 compared with model group, #P<0.05 compared with kuh-seng Radix Glycyrrhizae group
The experimental results showed that：Mouse give high lipid food feed 12 weeks after, mouse weight dramatically increases, Lee ' s indexes with
Blank group has significant difference (P<0.05), mouse figure is significantly fat, and liver weight and liver index have statistics with blank group
Difference (P<0.05)；After model mouse gives kuh-seng mixture glycyrrhiza, mouse weight significantly reduces, and mouse obesity Lee ' s indexes reduce
And there is significant difference (P with model group<0.05), liver weight and liver index more significantly reduce with model group and with statistics
Learn difference (P<0.05), while compared with blank group, Lee ' s indexes, liver weighs and the equal no difference of science of statistics of liver index.And give hardship
Mouse weight after ginseng decocting liquid or Licorice root, weight (g), Lee ' s indexes, liver weight (g), liver index index are extensive
It is multiple, but compare with kuh-seng mixture glycyrrhiza group that there are still significant differences.
2. kuh-seng Radix Glycyrrhizae prescription can significantly reduce model mouse blood glucose, blood lipids index
The serum of mouse is detected on full automatic biochemical apparatus.This experiment uses colorimetric determination serum total cholesterol
(TC), high-density lipoprotein (HDL), the horizontality of low-density lipoprotein (LDL), triglyceride (TG).
As a result：
**P<0.05 compared with blank group, * P<0.05 compared with model group, #P<0.05 compared with kuh-seng Radix Glycyrrhizae group
The experimental results showed that：After mouse gives high lipid food nursing 12 weeks, lipid of mice index TC, TG, LDL significantly rise
Height, and HDL is significantly reduced, blood lipids index all has significant difference (P with blank group<0.05), while mouse blood sugar is horizontal apparent
Increase (P<0.05)；After model mouse gives kuh-seng mixture glycyrrhiza, lipid of mice index TC, TG, LDL are significantly reduced and and model group
With significant difference (P<0.05), kuh-seng mixture glycyrrhiza simultaneously can significantly raised body HDL it is horizontal and reduce blood glucose level
And there is significant difference (P with model group<0.05).And mouse blood sugar after kuh-seng decocting liquid or Licorice root is given, blood fat
Index is restored, but compares with kuh-seng mixture glycyrrhiza group that there are still significant differences.
3. Pathological experiment shows that kuh-seng Radix Glycyrrhizae prescription significantly improves the change of liver fat venereal disease and inflammatory lesion
Light microscopy checking:Liver organization, 10% formaldehyde is taken to fix 24 hours, routine paraffin wax embedded section, HE dyeing, conventional liver
Dirty pathological observation.Oil red O stain:Frozen tissue section, 10% formaldehyde calcipexy 10 minutes are added 60% isopropanol and wash 30
Second, oil red O stostes 6ml stands 10 minutes after adding distilled water 4ml mixings, filters into sealing container, isopropanol is washed 30 seconds.Haematoxylin
Core is contaminated, glycerin gelatine mounting observes fatty infiltration of liver situation.Remaining biochemical detection is detected using kit.
A.H&E coloration results
Liver HE dyeing testing result normally organizes that the arrangement of mouse liver cell rope is normal, and liver cell form is normal, core circle, it is big,
Between two parties, it is accumulated without fat drips in endochylema, no inflammation cellular infiltration in leaflet.Model group rats leaflet structure is disorderly, degeneration of liver cells
With the most apparent around central vein, cell volume increases, and has a large amount of small fat drips vacuolizations and a small amount of big vacuole, core in endochylema
Side is occupied, has no liver fibrosis.Kuh-seng group, Radix Glycyrrhizae group mouse is reduced than model group fat drips vacuolization and vacuole reduces, and
Kuh-seng mixture glycyrrhiza group is given, mouse liver fat drips vacuolization significantly reduces, and there is only a small amount of small fat drips vacuole, inflammation is thin
Born of the same parents' infiltration is not significantly different with blank group.
B. oil red coloration result
Microscopic observation hepatic tissue fat is dyed through oil red, and model group mouse liver cell is denaturalized based on bubble property fat drips, largely
Red dye, kuh-seng group and Radix Glycyrrhizae group have reduction effect to mouse liver fat drips, are dyed for a large amount of small fat drips, and kuh-seng mixture glycyrrhiza
Group mouse liver cell denaturation substantially reduces and only deposits a small amount of red dye of vesicle fat drips.
1.2 research《The nonalcoholic fatty liver mouse model that different ratio kuh-seng Radix Glycyrrhizae prescription induces high lipid food
It influences》
Purpose and meaning：Study influence of the different ratio kuh-seng Radix Glycyrrhizae prescription to NAFLD mouse liver functions.
Method：C57BL/6J male mices (20-22g), are randomly divided into 5 groups, every group 10, high in fat in addition to blank group (A)
After diet modeling 4 weeks, high fat diet (is purchased from Nantong Te Luofei feed corporation,Ltds, code T P26301).Gastric infusion
(B) model group gives physiological saline gavage；(C) group gives kuh-seng Radix Glycyrrhizae prescription water decoction (1:0.5) gavage；(D) group gives hardship
Join Radix Glycyrrhizae gavage (1:1)；(E) group gives kuh-seng Radix Glycyrrhizae gavage (1:2) it after, continuation HFD feeds and is administered 12 weeks, puts to death, detection
Mouse liver function index of correlation.
1. different ratio kuh-seng Radix Glycyrrhizae prescription is to model mouse weight, the influence of liver weight；
The situation of ingesting for recording mouse weekly, records the weight of mouse every 2 weeks.Experiment terminate first 1 day, to mouse take every
Curfew eats.Second day detects the empty body weight and height of mouse, and calculates Lee ' s indexes：Lee ' s indexes=weight (g) 1/
3*1000* heights (cm) -1.Vena orbitalis posterior clump blood sampling is carried out to all mouse, detaches serum.It is rapid to put to death after mouse anesthesia
And detach liver organization.Then liver index is calculated：Liver index=liver weight (g)/empty body weight (g) * 100%.Experiment need to be left and taken
Left lobe of liver tissue, and a part therein is fixed on 10% neutral formalin solution kind, remain pathological analysis and reality
It tests.
As a result：
**P<0.05 compared with blank group, * P<0.05 compared with model group, #P<0.05 with kuh-seng Radix Glycyrrhizae (1:1) group compares
The experimental results showed that：Mouse give high lipid food feed 12 weeks after, mouse weight dramatically increases, Lee ' s indexes with
Blank group has significant difference (P<0.05), mouse figure is significantly fat, and liver weight and liver index have statistics with blank group
Difference (P<0.05)；After model mouse gives different ratio kuh-seng mixture glycyrrhiza, mouse weight significantly reduces, mouse obesity Lee ' s
Index reduces and all has significant difference (P with model group<0.05), liver weight and liver index are more significantly reduced with model group
And there is significant difference (P<0.05), wherein kuh-seng Radix Glycyrrhizae matches 1:1 group compared with blank group, Lee ' s indexes, liver weight and liver
The equal no difference of science of statistics of index.And give kuh-seng decocting liquid (1:0.5 or 1:2) mouse weight after group, weight (g), Lee ' s refer to
Number, liver weight (g), liver index index and kuh-seng mixture glycyrrhiza group (1:1) group compares that there are still significant difference (P<0.05).
2. different ratio kuh-seng Radix Glycyrrhizae prescription is to model mouse blood glucose, the influence of blood lipids index
The serum of mouse is detected on full automatic biochemical apparatus.This experiment uses colorimetric determination serum total cholesterol
(TC), high-density lipoprotein (HDL), the horizontality of low-density lipoprotein (LDL), triglyceride (TG).
As a result：
**P<0.05 compared with blank group, * P<0.05 compared with model group, #P<0.05 with kuh-seng Radix Glycyrrhizae group (1:1) compare
The experimental results showed that：After mouse gives high lipid food nursing 12 weeks, lipid of mice index TC, TG, LDL significantly rise
Height, and HDL is significantly reduced, blood lipids index all has significant difference (P with blank group<0.05), while mouse blood sugar is horizontal apparent
Increase (P<0.05)；After model mouse gives different ratio kuh-seng mixture glycyrrhiza, small blood lipids index TC, TG, LDL significantly reduce and with
Model group has significant difference (P<0.05), kuh-seng mixture glycyrrhiza simultaneously can significantly raised body HDL it is horizontal and reduce blood
Sugar level and with model group have significant difference (P<0.05).Wherein give kuh-seng decocting liquid (1:1) after group with blank group ratio
Compared with HDL is not significantly different, and gives kuh-seng decocting liquid (1:0.5 or 1:2) mouse after group, TG, LDL, HDL and kuh-seng Radix Glycyrrhizae
Mixture (1:1) group, which is compared, has significant difference (P<0.05).
3. different ratio kuh-seng Radix Glycyrrhizae prescription becomes to liver fat venereal disease and the influence of inflammatory lesion
Light microscopy checking:Liver organization, 10% formaldehyde is taken to fix 24 hours, routine paraffin wax embedded section, HE dyeing, conventional liver
Dirty pathological observation.Oil red O stain:Frozen tissue section, 10% formaldehyde calcipexy 10 minutes are added 60% isopropanol and wash 30
Second, oil red O stostes 6ml stands 10 minutes after adding distilled water 4ml mixings, filters into sealing container, isopropanol is washed 30 seconds.Haematoxylin
Core is contaminated, glycerin gelatine mounting observes fatty infiltration of liver situation.Remaining biochemical detection is detected using kit.
A.H&E is dyed and oil red O stain result
Liver HE dyeing testing result normally organizes that the arrangement of mouse liver cell rope is normal, and liver cell form is normal, core circle, it is big,
Between two parties, it is accumulated without fat drips in endochylema, no inflammation cellular infiltration in leaflet.Model group rats leaflet structure is disorderly, degeneration of liver cells
With the most apparent around central vein, cell volume increases, and has a large amount of small fat drips vacuolizations and a small amount of big vacuole, core in endochylema
Side is occupied, has no liver fibrosis.The mouse liver for giving different kuh-seng Radix Glycyrrhizae prescriptions is subtracted than the vacuolization of model group fat drips
Less and vacuole reduces, wherein kuh-seng Radix Glycyrrhizae (1:1) group effect is the most notable.Oil red O stain, which is shown, gives different kuh-seng Radix Glycyrrhizae groups
The mouse liver of side is formed than model group fat drips to be reduced, and compared to the large area red staining of model, administration group is
The small red dye of fat drips, and wherein kuh-seng Radix Glycyrrhizae (1:1) group effect is the most notable.
Embodiment 2 is studied《The nonalcoholic fatty liver disease mouse model that kuh-seng Radix Glycyrrhizae induces methionine deficiency feed
Influence》
2.1 research《The nonalcoholic fatty liver disease mouse mould that kuh-seng Radix Glycyrrhizae induces methionine deficiency feed with folk prescription
The influence of type》
Purpose and meaning：Study kuh-seng Radix Glycyrrhizae prescription, the influence of kuh-seng, Radix Glycyrrhizae to NAFLD mouse liver functions.
Method：C57BL/6J male mices (20-22g), are randomly divided into 5 groups, every group 10, in addition to blank group (A), egg ammonia
After feed (MCD) diet modeling that acid heat 1 week, methionine deficiency feed (is purchased from Nantong Te Luofei feed corporation,Ltds, compiles
Number TP3005G).Gastric infusion (B) model group gives physiological saline gavage；(C) group gives kuh-seng Radix Glycyrrhizae prescription water decoction gavage；
(D) group gives kuh-seng gavage；(E) group is given Radix Glycyrrhizae gavage and is put to death after continuation MCD feeds and is administered 4 weeks, detects mouse liver function
It can index of correlation.
1. kuh-seng Radix Glycyrrhizae prescription can reduce model mouse ALT, AST and inflammatory factor is horizontal；
The serum of mouse is detected on full automatic biochemical apparatus.This experiment uses glutamic-pyruvic transaminase in colorimetric determination serum
(ALT), glutamic-oxalacetic transaminease (AST), tumor necrosis factor (TNF-α), interleukin 6 (IL-6), IL-10 (IL-10) water
It is flat.
As a result：
**P<0.05 compared with blank group, * P<0.05 compared with model group, #P<0.05 compared with kuh-seng Radix Glycyrrhizae group
The experimental results showed that：Model mouse is significantly increased compared to blank control mouse, Serum ALT, AST indexs；And cause inflammation
Factor level TNF-α, IL-6 are significantly increased, and the correlation factor IL-10 that inflammation occurs is inhibited to be substantially reduced, and give kuh-seng Radix Glycyrrhizae
After mixture, mice serum ALT, AST level significantly reduces, and is not significantly different with blank group.Mice serum inflammatory factor water
Flat to significantly improve, it is horizontal that kuh-seng mixture glycyrrhiza significantly reduces Serum TNF-α, IL-6, and it is horizontal to increase IL-10, and with model group ratio
More significant difference (P < 0.05), wherein IL-10 levels are not significantly different with blank group.Kuh-seng group and Radix Glycyrrhizae group pair
Mice serum ALT, AST are horizontal and inflammatory factor TNF-α, IL-6, IL-10 all have certain improvement result, but kuh-seng group and hardship
Ginseng mixture glycyrrhiza group is compared with significant difference, and Radix Glycyrrhizae group is to the improvement result of TNF-α with statistics compared with mixture group
Difference (P < 0.05).
2. experiment mice pathology of hepar detects
Light microscopy checking:Liver organization, 10% formaldehyde is taken to fix 24 hours, routine paraffin wax embedded section, HE dyeing, conventional liver
Dirty pathological observation.Oil red O stain:Frozen tissue section, 10% formaldehyde calcipexy 10 minutes are added 60% isopropanol and wash 30
Second, oil red O stostes 6mL stands 10 minutes after adding distilled water 4mL mixings, filters into sealing container, isopropanol is washed 30 seconds.Haematoxylin
Core is contaminated, glycerin gelatine mounting observes fatty infiltration of liver situation.Remaining biochemical detection is detected using kit.
A.H&E coloration results
Murine liver tissue lobuli hepatis is regular, sinus hepaticus is high-visible, liver rope marshalling.Liver cell normal in size, center can
See big and justifies
Core, cytoplasm uniform coloring.It is deposited without fat drips in liver cell, morphosis is normal, fat-free change and inflammation
Change；MCD group murine liver tissue lobuli hepatis obscurity boundary, sinus hepaticus narrow, and it is disorderly that liver Cable Structure occurs in small part region.Liver cell
Swelling, boundary are unclear.The visible large stretch of vacuole sample fat drips of intracellular, not of uniform size, the cell that steatosis occurs is distributed in stove shape.It gives
It gives kuh-seng mixture glycyrrhiza and substantially reduces the visible large stretch of vacuole sample fat drips of intracellular, tiny type vacuole sample fat drips are presented, inflammatory infiltration subtracts
It is few；And kuh-seng group or Radix Glycyrrhizae group have certain curative effect, but effect is not as good as kuh-seng Radix Glycyrrhizae group.
B. oil red coloration result
Microscopic observation hepatic tissue fat is dyed by oil red, and the denaturation of model group mouse liver cell is with big vacuolated fat drips
It is main, significant quantities of fat red staining is organized, and mouse liver cell is denaturalized then based on vesicle fat drips after giving kuh-seng mixture glycyrrhiza.
And kuh-seng group or Radix Glycyrrhizae group have certain curative effect, but effect is not as good as kuh-seng Radix Glycyrrhizae group.
2.2 research《The nonalcoholic fatty liver disease mouse that different ratio kuh-seng Radix Glycyrrhizae induces methionine deficiency feed
The influence of model》
Purpose and meaning：Study influence of the different ratio kuh-seng Radix Glycyrrhizae prescription to NAFLD mouse liver functions.
Method：C57BL/6J male mices (20-22g), are randomly divided into 5 groups, every group 10, in addition to blank group (A), egg ammonia
After feed (MCD) diet modeling that acid heat 1 week, methionine deficiency feed (is purchased from Nantong Te Luofei feed corporation,Ltds, compiles
Number TP3005G).Gastric infusion (B) model group gives physiological saline gavage；(C) group gives kuh-seng Radix Glycyrrhizae prescription water decoction (1:
0.5) gavage；(D) group gives kuh-seng Radix Glycyrrhizae gavage (1:1)；(E) group gives kuh-seng Radix Glycyrrhizae gavage (1:2), continue MCD to feed simultaneously
It after being administered 4 weeks, puts to death, detects mouse liver function index of correlation.
1. kuh-seng Radix Glycyrrhizae prescription can reduce model mouse ALT, AST and inflammatory factor is horizontal；
The serum of mouse is detected on full automatic biochemical apparatus.This experiment uses glutamic-pyruvic transaminase in colorimetric determination serum
(ALT), glutamic-oxalacetic transaminease (AST), tumor necrosis factor (TNF-α), interleukin 6 (IL-6), IL-10 (IL-10) water
It is flat.
As a result：
**P<0.05 compared with blank group, * P<0.05 compared with model group, #P<0.05 compared with kuh-seng Radix Glycyrrhizae group
The experimental results showed that：Model mouse is significantly increased compared to blank control mouse, Serum ALT, AST indexs；And cause inflammation
Factor level TNF-α, IL-6 are significantly increased, and the correlation factor IL-10 that inflammation occurs is inhibited to be substantially reduced, and give different ratio
After kuh-seng mixture glycyrrhiza, mice serum ALT, AST level significantly reduces.Mice serum inflammatory factor level significantly improves, bitter
Join mixture glycyrrhiza (1:0.5), (1:2) it is horizontal that Serum TNF-α, IL-6 can be significantly reduced, increase IL-10 levels, but and kuh-seng
Mixture glycyrrhiza (1:1) the still significant difference (P < 0.05) of rating unit index is organized.
2. experiment mice pathology of hepar detects
Light microscopy checking:Liver organization, 10% formaldehyde is taken to fix 24 hours, routine paraffin wax embedded section, HE dyeing, conventional liver
Dirty pathological observation.Oil red O stain:Frozen tissue section, 10% formaldehyde calcipexy 10 minutes are added 60% isopropanol and wash 30
Second, oil red O stostes 6mL stands 10 minutes after adding distilled water 4mL mixings, filters into sealing container, isopropanol is washed 30 seconds.Haematoxylin
Core is contaminated, glycerin gelatine mounting observes fatty infiltration of liver situation.Remaining biochemical detection is detected using kit.
A.H&E is dyed and oil red coloration result
Murine liver tissue lobuli hepatis is regular, sinus hepaticus is high-visible, liver rope marshalling.Liver cell normal in size, center can
See big and circle core, cytoplasm uniform coloring.It is deposited without fat drips in liver cell, morphosis is normal, fat-free change and inflammation
Change；MCD group murine liver tissue lobuli hepatis obscurity boundary, sinus hepaticus narrow, and it is disorderly that liver Cable Structure occurs in small part region.Liver cell
Swelling, boundary are unclear.The visible large stretch of vacuole sample fat drips of intracellular, not of uniform size, the cell that steatosis occurs is distributed in stove shape.It gives
The visible large stretch of vacuole sample fat drips of intracellular can be effectively reduced by giving the kuh-seng mixture glycyrrhiza of different ratio, and tiny type vacuole sample fat is presented
Drop, inflammatory infiltration are reduced；Wherein kuh-seng Radix Glycyrrhizae proportioning (1:1) group is the most notable, carries out fat drips dyeing display using oil red O, no
Kuh-seng mixture glycyrrhiza with proportioning can effectively reduce the visible fat drips red staining of intracellular, wherein kuh-seng Radix Glycyrrhizae prescription (1:1) group
Effect is the most notable.
The above description is merely a specific embodiment, but scope of protection of the present invention is not limited thereto, any
Those familiar with the art in the technical scope disclosed by the present invention, can easily think of the change or the replacement, and should all contain
Lid is within protection scope of the present invention.Therefore, protection scope of the present invention should be based on the protection scope of the described claims.
Claims (6)
1. the kuh-seng Radix Glycyrrhizae prescription for treating non-alcohol fatty liver, it is characterised in that：Kuh-seng, Radix Glycyrrhizae weight proportion
For：1：0.1~5.
2. the kuh-seng Radix Glycyrrhizae prescription according to claim 1 for treating non-alcohol fatty liver, it is characterised in that：
Kuh-seng, Radix Glycyrrhizae weight proportion be：1：1.
3. the kuh-seng Radix Glycyrrhizae prescription according to claim 1 for treating non-alcohol fatty liver, it is characterised in that：
The kuh-seng, Radix Glycyrrhizae composition form include the work of kuh-seng, traditional Chinese medicine of licorice root material or kuh-seng, the extract of Radix Glycyrrhizae or kuh-seng, Radix Glycyrrhizae
Property monomer.
4. the kuh-seng Radix Glycyrrhizae prescription according to claim 3 for treating non-alcohol fatty liver, it is characterised in that：
The kuh-seng, Radix Glycyrrhizae extract include water extract or alcohol extract.
5. the kuh-seng Radix Glycyrrhizae prescription according to claim 3 for treating non-alcohol fatty liver, it is characterised in that：
The activated monomer of the kuh-seng includes matrine, oxymatrine, and the activated monomer of Radix Glycyrrhizae includes glycyrrhizic acid, enoxolone and its
The chemical derivative of activated monomer.
6. the kuh-seng Radix Glycyrrhizae prescription according to claim 1 for treating non-alcohol fatty liver, it is characterised in that：
The dosage form of the kuh-seng Radix Glycyrrhizae prescription is tablet, pill, particle, capsule or injection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811083160.1A CN108743656A (en) | 2018-09-17 | 2018-09-17 | Kuh-seng Radix Glycyrrhizae prescription for treating non-alcohol fatty liver |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811083160.1A CN108743656A (en) | 2018-09-17 | 2018-09-17 | Kuh-seng Radix Glycyrrhizae prescription for treating non-alcohol fatty liver |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108743656A true CN108743656A (en) | 2018-11-06 |
Family
ID=63968152
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811083160.1A Pending CN108743656A (en) | 2018-09-17 | 2018-09-17 | Kuh-seng Radix Glycyrrhizae prescription for treating non-alcohol fatty liver |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108743656A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111012847A (en) * | 2020-01-07 | 2020-04-17 | 包纳日斯 | Mongolian medicine for treating non-alcoholic fatty liver and preparation method thereof |
| CN112206231A (en) * | 2020-10-10 | 2021-01-12 | 中国医学科学院医药生物技术研究所 | Application of matrine derivative in treating non-alcoholic steatohepatitis |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104857070A (en) * | 2014-02-24 | 2015-08-26 | 贺平 | Pharmaceutical composition used for resisting intestinal tract inflammatory injuries, and preparation method and applications thereof |
| CN106074681A (en) * | 2016-06-17 | 2016-11-09 | 中国人民解放军第四军医大学 | A kind of dispersible tablets of Chinese medicine of anti-hepatic fibrosis and preparation method thereof |
| CN108498570A (en) * | 2017-02-28 | 2018-09-07 | 中国人民解放军第四军医大学 | A kind of Chinese medicine compound prescription and preparation method thereof for anti-idiopathic pulmonary fibrosis |
-
2018
- 2018-09-17 CN CN201811083160.1A patent/CN108743656A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104857070A (en) * | 2014-02-24 | 2015-08-26 | 贺平 | Pharmaceutical composition used for resisting intestinal tract inflammatory injuries, and preparation method and applications thereof |
| CN106074681A (en) * | 2016-06-17 | 2016-11-09 | 中国人民解放军第四军医大学 | A kind of dispersible tablets of Chinese medicine of anti-hepatic fibrosis and preparation method thereof |
| CN108498570A (en) * | 2017-02-28 | 2018-09-07 | 中国人民解放军第四军医大学 | A kind of Chinese medicine compound prescription and preparation method thereof for anti-idiopathic pulmonary fibrosis |
Non-Patent Citations (1)
| Title |
|---|
| 张庆山等: "苦参素胶囊联合甘草酸二铵对非酒精脂肪肝患者炎性因子及肝功能的影响", 《中国中药杂志2015/专集：基层医疗机构从业人员科技论文写作培训会议论文集》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111012847A (en) * | 2020-01-07 | 2020-04-17 | 包纳日斯 | Mongolian medicine for treating non-alcoholic fatty liver and preparation method thereof |
| CN112206231A (en) * | 2020-10-10 | 2021-01-12 | 中国医学科学院医药生物技术研究所 | Application of matrine derivative in treating non-alcoholic steatohepatitis |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101091765A (en) | Medicinal comsns-and usage for preventing and treating diabets mellitus | |
| CN102091083A (en) | Petroleum ether extract of traditional Chinese medicine for preventing and treating glucose and lipid metabolic disturbance and preparation method thereof | |
| CN108743656A (en) | Kuh-seng Radix Glycyrrhizae prescription for treating non-alcohol fatty liver | |
| CN101069710A (en) | Compounded medicine for preventing and treating metabolic syndrome and hyperlimemia | |
| CN101874832A (en) | Wine-steamed Chinese goldthread processed product as well as preparation method and application thereof | |
| CN103800352A (en) | Compound traditional Chinese medicinal effective component preparation for resisting alcoholic fatty liver disease and application thereof | |
| CN107115338A (en) | Application of the bicyclic alcohols in diabetes medicament is prepared | |
| CN109620831A (en) | Application of the crocetin in preparation treatment nonalcoholic steatohepatitis drug | |
| CN101816698B (en) | Composition for preventing and curing metabolism disturbance syndrome | |
| CN100579564C (en) | Medicine for curing gout and its preparing method | |
| CN104666890A (en) | Traditional Chinese medicine preparation capable of reducing blood sugar | |
| CN110613792B (en) | Traditional Chinese medicine composition with blood sugar reducing effect and preparation method and application thereof | |
| CN100534461C (en) | Pharmaceutical composition for treating diabetes and impaired glucose tolerance and preparation method thereof | |
| CN103055176A (en) | Traditional Chinese medicine for treating diabetes mellitus and preparation method thereof | |
| CN107184831B (en) | Traditional Chinese medicine composition for treating hyperlipidemia and preparation method thereof | |
| CN103272146B (en) | Medicine composition used for preventing and treating alcoholic fatty liver and preparation method thereof | |
| CN105920051A (en) | Needle mushroom extract and preparation method and application thereof | |
| CN101269152A (en) | Application of matrimony vine and black fungus in preparing fatty liver resistant medicament | |
| CN106018776A (en) | Experiment analysis method for blood glucose reducing efficacy of Shenlian decoction rapidly disintegrating tablets for reducing blood glucose | |
| CN101120969A (en) | Medicine for treating diabetes and its complications and preparing method thereof | |
| CN101874830B (en) | New application of golden thread processed product | |
| CN111840331A (en) | Application of bear gall exosome in preparation of medicine for treating type II diabetes | |
| CN104352748A (en) | Traditional Chinese medicine composition for treating diabetic nephropathy and preparation method thereof | |
| KR20030059951A (en) | Composition containing an extract of sopungsungi-won crude drug complex for preventing and treating diabetes mellitus | |
| US20220079975A1 (en) | Method for mitigation of non-alcoholic fatty liver disease by use of a composition comprising small-molecule fucoidan and fucoxanthin |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20181106 |