CN108740145A - One kind is relieved the effect of alcohol black tea and preparation method thereof - Google Patents
One kind is relieved the effect of alcohol black tea and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23F3/00—Tea; Tea substitutes; Preparations thereof
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Abstract
The present invention provides one kind and relieves the effect of alcohol black tea and preparation method thereof, includes mainly leaf picking, wither, once rub, spray edible mushroom complex polysaccharide liquid, secondary rub, one time fermentation, spray edible mushroom composite enzyme liquid, rub three times, secondary fermentation, baking step.Wherein, in the preparation process of edible mushroom complex polysaccharide liquid and edible mushroom composite enzyme liquid, it is preferred that the edible mushroom of both specific combinations of needle mushroom and mushroom is raw material, edible fungi polysaccharide and edible mushroom ferment with good sober-up function is made by enzymolysis, the two substep is sprayed on tealeaves simultaneously, so that tealeaves is absorbed above-mentioned polysaccharide and ferment ingredient, to be incorporated in the preparation process of black tea, the tea product form that consumer accepts extensively is made.It brews after drinking for antialcoholism action, there is synergistic function, not only significantly extend drunk incubation period, shorten the time of sobering up, also act as the effect of liver protection attenuation, and compared with the prior art, have effects that more preferably Antialcoholic liver-protecting.
Description
Technical field
The invention belongs to black tea technical fields, and in particular to a kind of preparation of the black tea with Antialcoholic liver-protecting and other effects.
Background technology
Spirits culture occupies unique status in Chinese traditional culture, but drunk and alcoholism also increasingly becomes China
Public health problem.Acute heavy drinking can cause Nausea and vomiting, failure of memory, absent minded etc., and long-term
The diseases such as phrenoblabia, gastric ulcer and fatty liver, alcoholic hepatitis, hepatic sclerosis will be led to by drinking.Therefore, how alcohol is reduced
Damage to health is one of current International Medical and the hot spot of biological study, how to develop effective relieving alcoholism and protecting liver
Class health food, has also had received universal attention, especially Small side effects, the rapid liver-protective solution drinks of energy again of effect
The market prospects of health products are quite optimistic.
And relieved the effect of alcohol with tea, it is that people know well and are widely used in daily life already, tea polyphenols, egg is contained in tealeaves
The Multiple components such as white matter, amino acid, alkaloid, carbohydrate, vitamin and enzyme, the ingredients such as tea polyphenols therein, caffeine can carry
High liver promotes blood circulation to the metabolism of drug, there is diuresis, can by human blood alcohol and its metabolism produce
Object is discharged from urine, mitigates and eliminate the toxic side effect brought due to alcohol.Black tea is traditional one of the six big teas in China, is belonged to
Full fermentation tea, traditional tea-drinking product of feature and daily life with fragrant and sweet taste alcohol.
Ferment is a kind of enzyme with special bioactivity being made of amino acid, it is present in the animal and plant body of all work
It is interior, it is to maintain body normal function, digests food, a kind of necessary material of the vital movements such as repair tissue.Ferment participates in all
Vital movement, if without ferment, biochemical reaction will be unable to carry out.The prior art has been found that the ferment that relieves the effect of alcohol can be by protecting liver
Cell stablizes liver plasma membrane, to inhibit glutamic-pyruvic transaminase in hepatic tissue (Alanine aminotransferase, ALT), paddy
The raising of careless transaminase (Aspartate transaminase, AST) inhibits ALT, AST to be released into blood, to effectively prevent wine
Essence hepatic injury.
Edible fungi polysaccharide is a kind of special bioactive substance, has various health care functions, such as it is as a kind of life
The agent of object increased response and conditioning agent can enhance humoral immunity and cellular immune function.The Antiviral mechanism of edible fungi polysaccharide
It may is that it improves infection cell immunity, enhance the stability of cell membrane, inhibit cytopathy, promote the work(such as cell repair
Energy.Meanwhile edible fungi polysaccharide also has anti-retroviral activity.
Flammulina velutipes are one of needle mushroom main actives, and many researchs are thought, flammulina velutipes, which not only have, to be carried
High body immunity, and with the health cares such as liver protection, moisturizing, anti-infective, anti-oxidant, the gentle physical fatigue of auxiliary improvement of memory
Effect.
Lentinan is the effective active composition extracted from mushroom fruiting body, is the principle active component of mushroom, simultaneously
It is also a kind of host immune reinforcing agent, it is clinical to show that lentinan has antiviral and antitumor, adjusting immune with pharmacological research
The effects that function and stimulation interferon formation.
Although in the prior art, existing lot of documents is disclosed using edible mushroom as the preparation of the hangover-alleviating health-care food of raw material,
But for current overall technology situation, there is such as problems in such health food：（1）On product form, mostly capsule,
Granule, tablet, oral solution etc. are usually the conventional dosage forms of drug, and product pattern is more single, lack more close tradition meaning
Type of food in justice, such as patent CN101156877A etc..（2）In raw material selection, select raw material various but more random,
Although be involved in the prior art edible fungi polysaccharide/edible mushroom ferment for health products application, and by the two combine into
The application of row application, such as patent CN106578801A, CN106579384A etc., but feature and difference not yet to raw material respectively
Interaction etc. between combination is further excavated, also without being applied for specific the effect of relieving the effect of alcohol, more not from raw material
Combination, edible fungi polysaccharide and edible mushroom ferment the angle of combination start with to solve the problems, such as.（3）In preparation process, although public
There are many preparation method of the edible fungi polysaccharide and edible mushroom ferment opened, have disclosure also for the feedstock processing of edible mushroom more, such as
Patent CN103859538A, CN105707851A etc., but both polysaccharide and ferment are mostly to carry out preparation method each independently, not
Both make full use of the similitude on raw material and processing step recombinated, uniformly, simplify, while preparation method uses mostly
Be general processing step, and the edible fungi raw materials of specific combination are not selected with targetedly process conditions and parameter
Optimization.
Invention content
In order to overcome the above-mentioned deficiencies of the prior art, it relieves the effect of alcohol black tea and its preparation side the purpose of the present invention is to provide one kind
Method, it is final to determine with the edible of needle mushroom and mushroom both specific combinations by the independent and combination experiment of multiple eating bacterium
Bacterium is raw material, through supersonic synergic enzymolysis process, then prepares edible mushroom complex polysaccharide and edible mushroom composite enzyme respectively, and melted
In the preparation process for entering black tea, the tea product form that consumer accepts extensively is made, brew after drinking for antialcoholism action and
Speech has synergistic function, not only significantly extends drunk incubation period, shortens the time of sobering up, and also acts as liver protection attenuation
Effect, and compared with the prior art, have effects that more preferably Antialcoholic liver-protecting.
Specifically, the present invention is achieved through the following technical solutions；First purpose of the present invention, is to provide one kind
Relieve the effect of alcohol black tea, and preparation process is：Leaf picking, wither, spray edible mushroom complex polysaccharide liquid, rub, one time fermentation, sprinkling it is edible
Bacterium composite enzyme liquid is rubbed, secondary fermentation, manage bar, baking step again.
Second object of the present invention is to provide a kind of preparation method for the black tea that relieves the effect of alcohol, and concrete operation step is：
（1）Sorting：Fresh leaves of tea plant is picked as raw material；
（2）It withers：It is 60-75% fresh leaf uniformly to be spread on withering trough, withering to fresh leaf moisture content；
（3）Once rub：Withering leaf is placed in rolling machine, rubbed with pneumatics-light kneadding twirl-pneumatics rubs-weight rubs
Sequentially, carry out rubbing operation successively, until tea juice start it is excessive, preliminarily form strip；
（4）Spray edible mushroom complex polysaccharide liquid：Withering leaf is placed in blender, under stirring, complex polysaccharide liquid is pressed
According to 200-500mL/kg tealeaves ratio uniform be sprayed on withering leaf, and be uniformly mixed；
（5）It is secondary to rub：Uniform tealeaves will be sprayed to be placed in rolling machine, using the mode of rubbing gently rubbed, gently rub 10-20min；
（6）One time fermentation：Tealeaves is gently fermented under closed environment, 25-35 DEG C of room temperature of fermentation, fermentation time 5-10h；
（7）Spray edible mushroom composite enzyme liquid：One time fermentation leaf is placed in blender, under stirring, by composite enzyme
Liquid according to 50-100mL/kg tealeaves ratio uniform be sprayed on one time fermentation leaf, and be uniformly mixed；
（8）It rubs three times：Uniform tealeaves will be sprayed to be placed in rolling machine, using the mode of rubbing gently rubbed, gently rub 5-10min；
（9）Secondary fermentation：Tealeaves is gently fermented under closed environment again, 25-35 DEG C of room temperature of fermentation, fermentation time
0.5-5h；
（10）Drying：By the way of low temperature drying, 50-80 DEG C of primary drying for baking of secondary fermentation leaf elder generation is dried into 0.5-3h, then 60-90 DEG C
Final firing Titian 5-30min to get.
Preferably, the preparation method of the edible mushroom complex polysaccharide liquid and edible mushroom composite enzyme liquid is as follows：
1）Feedstock processing：Needle mushroom and mushroom are taken, by the two amount ratio（1-4）:1 mixing adds water mashing that edible mushroom composite pulp is made；
2）Supersonic synergic enzymolysis processing：By step 1）Edible mushroom composite pulp be placed in ultrasonic extractor carry out ultrasonic wave at
It manages, complex enzyme zymohydrolysis is added in composite pulp after treatment, heat up enzyme deactivation, obtains edible mushroom enzymolysis liquid；
3）The preparation of complex polysaccharide liquid：Take step 2）Edible mushroom enzymolysis liquid, it is compound that edible mushroom is made after extracted complex polysaccharide
Polysaccharide liquid；
4）The preparation of composite enzyme liquid：Take step 2）Edible mushroom enzymolysis liquid, edible mushroom composite enzyme liquid is made through inoculation fermentation.
It is further preferred that the specific preparation method of the edible mushroom complex polysaccharide liquid and edible mushroom composite enzyme liquid is such as
Under：
1）Feedstock processing：Needle mushroom and mushroom are taken, by the two amount ratio（1-4）:1 mixing adds water mashing that edible mushroom composite pulp is made；
2）Supersonic synergic enzymolysis processing：By step 1）Edible mushroom composite pulp be placed in ultrasonic extractor carry out ultrasonic wave at
Reason, 30-60min is handled under the conditions of 300-800W, 30-50 DEG C；0.05-0.2% is added in composite pulp that treated（w/w）It is multiple
Synthase digests 1-3h under the conditions of pH 5-7,30-50 DEG C, and heat up enzyme deactivation, obtains edible mushroom enzymolysis liquid；
3）The preparation of complex polysaccharide liquid：Take step 2）Edible mushroom enzymolysis liquid, through centrifuge, take supernatant, concentration, be added ethyl alcohol it is quiet
It sets, precipitation is collected by centrifugation, is crushed after vacuum freeze drying, edible mushroom complex polysaccharide liquid is made after adding water dilution；
4）The preparation of composite enzyme liquid：Take step 2）Edible mushroom enzymolysis liquid, appropriate brown sugar is added, high-temperature sterilization after dissolving connects
Kind composite zymocyte, is placed in insulating box and ferments, and it is compound that edible mushroom is made through centrifuging, taking supernatant, after being diluted with water for zymotic fluid
Enzyme liquid.
It is further preferred that step 1）In, by needle mushroom and mushroom mass ratio 3:1 mixing adds water mashing that edible mushroom is made
Composite pulp, wherein amount of water are 10-15 times of mixture.
It is further preferred that step 2）In, the supersonic synergic enzymolysis processing, concrete operations are：By step 1）Food
It is placed in ultrasonic extractor with bacterium composite pulp and carries out ultrasonication, 45min is handled under the conditions of 600W, 40 DEG C；After processing
Composite pulp in add 0.1%（w/w）Complex enzyme digests 2h under the conditions of 6,35 DEG C of pH, and heat up enzyme deactivation, obtains edible mushroom enzymolysis
Liquid.
It is further preferred that step 2）In, the complex enzyme presses quality by flavor protease, cellulase and pectase
Than（2-5）:（2-5）:1 composition.
It is further preferred that step 2）In, the complex enzyme presses quality by flavor protease, cellulase and pectase
Than 4:4:1 composition.
It is further preferred that step 4）In, take step 2）Edible mushroom enzymolysis liquid, appropriate brown sugar, high temperature after dissolving is added
Sterilizing is inoculated with 0.5-1%（w/w）Composite zymocyte is placed in 20-30 DEG C of insulating box the 20-40d that ferments, and zymotic fluid is through centrifuging, taking
Supernatant, concentration, crush after vacuum freeze drying, obtain edible mushroom composite enzyme.
It is further preferred that step 4）In, take step 2）Edible mushroom enzymolysis liquid, appropriate brown sugar, high temperature after dissolving is added
Sterilizing, inoculation 1%（w/w）Composite zymocyte, is placed in 25 DEG C of insulating boxs the 30d that ferments, and zymotic fluid is through centrifuging, taking supernatant, dense
Contracting, crushes after vacuum freeze drying, obtains edible mushroom composite enzyme.
It is further preferred that step 4）In, the composite zymocyte by saccharomycete and Lactobacillus casei in mass ratio（3-
5）:1 composition.
It is further preferred that step 4）In, the composite zymocyte is by saccharomycete and Lactobacillus casei in mass ratio 4:1
Composition.
Compared with prior art, the present invention having the advantages that：（1）Selection for raw material, the present invention are preferably golden
The edible fungi raw materials of needle mushroom and mushroom combine, although needle mushroom, mushroom are very common on the market while being also that consumer is logical
The edible mushroom type of often feeding, although the precedent that the combination for having multiple eating bacterium in the prior art is prepared as raw material, although
Also it is no lack of the technical solution for being compounded using both needle mushroom and mushroom as major ingredient and preparing alcohol-neutralize healthy product, but this hair in the prior art
A bright technical contribution is exactly specifically to determine using needle mushroom and mushroom both edible mushrooms as raw material, is had complementary advantages, and goes forward side by side one
Step makes proportioning the most suitable, i.e., using raw material sources extensively, the lower-cost needle mushroom of addition and mushroom be as mainly accounting for
Than formula, the synergistic effect both given full play to, not only more preferably, and production cost substantially reduces relieving alcoholism and protecting liver effect, is more suitable for
Ordinary consumption group drinks.
（2）For be adapted to technique optimization, compared with the prior art in be mostly by both polysaccharide and ferment each independently
The mode prepared, another technical contribution point of the invention is to combine the preparation of the two, thin according to edible mushroom
The thicker feature of cell wall, targetedly first carries out enzymolysis broken wall, and active ingredient is made to be more easy to dissolve out, and utilizes the hole of ultrasonic wave
Effect promotes the process of its broken wall, then obtained enzymolysis liquid is prepared polysaccharide and ferment respectively, compared to individually carrying out both
The extraction of ingredient takes full advantage of the similitude on the two raw material and in preparation process, so that the process enormously simplifies
Technological operation step, and targetedly process conditions and parameter optimization are selected according to the edible fungi raw materials of specific combination,
Greatly improve the validity of technique.
（3）Form is taken for product, technical contribution of the invention point also resides in the imbibable form using black tea tea-drinking, and
The traditional leaf picking of black tea, wither, rub, ferment, stoving process on the basis of, by way of sprinkling, edible mushroom is answered
Close polysaccharide liquid and edible mushroom composite enzyme liquid and be sprayed onto tealeaves surface, and agitated mixing so that tealeaves absorb it is above-mentioned have relieve the effect of alcohol
The ingredient of liver-protecting efficacy is to greatly promote the corresponding dispelling effects of alcohol of black tea.Meanwhile in order to reach better assimilation effect, spraying
First tealeaves rub operation with appropriate destruction leaf texture, to the suction conducive to complex polysaccharide liquid/composite enzyme liquid before spilling
It receives, then further gently rubs after spraying and enable aforesaid liquid to travel further into tealeaves interior tissue and be evenly distributed, and pass through
It ferments and dries and be fixed on inside tealeaves.Based on this, the product form of health-care black tea is made in the present invention, on the one hand, its product
Appearance and drinking way are more closely similar to traditional food, and there are no contradict psychology by target user；And on the other hand, by will be above-mentioned independent
Specific combination is formed specifically to match using polysaccharide, the ferment for all having certain sober-up function, along with sheet of drinking tea
Body has certain dispelling effects of alcohol so that the black tea product being finally made, relieving alcoholism and protecting liver obvious effect improve, hence it is evident that are better than it
The dispelling effects of alcohol of exclusive use.
（4）For application prospect, by comparative experimental data it can be confirmed that the sobering-up health tea of the application not only has drink
The good result relieved the effect of alcohol after wine/drunk, while also there is the effect of pre- preventing drunkenness in advance.It not only significantly extends drunk latent
Phase shortens the time of sobering up, and also acts as the effect of liver protection attenuation, and compared with the prior art, has more preferably Antialcoholic liver-protecting
Effect.
Specific implementation mode
It is further illustrated the present invention below by specific implementation mode, following embodiment is the specific embodiment party of the present invention
Formula, but embodiments of the present invention are not limited by following embodiments.
Embodiment 1：
A kind of preparation method for the black tea that relieves the effect of alcohol, specific process step are：（1）Leaf picking：Fresh leaves of tea plant is picked as raw material；
（2）It withers：It is 70% fresh leaf uniformly to be spread on withering trough, withering to fresh leaf moisture content；（3）Once rub：Withering leaf is set
In rolling machine ,-the sequence rubbed of weight is rubbed-light kneadding twirl-pneumatics rubs with pneumatics, carries out rubbing operation successively, until tea
Juice start it is excessive, preliminarily form strip；（4）Spray edible mushroom complex polysaccharide liquid：Withering leaf is placed in blender, in stirring shape
Under state, by complex polysaccharide liquid according to 300mL/kg tealeaves ratio uniform be sprayed on withering leaf, and be uniformly mixed；（5）Two
It is secondary to rub：Uniform tealeaves will be sprayed to be placed in rolling machine, using the mode of rubbing gently rubbed, gently rub 15min；（6）One time fermentation：
Tealeaves is gently fermented under closed environment, 30 DEG C of room temperature of fermentation, fermentation time 8h；（7）Spray edible mushroom composite enzyme
Liquid：One time fermentation leaf is placed in blender, it is under stirring, composite enzyme liquid is equal according to the ratio of 100mL/kg tealeaves
It is sprayed on one time fermentation leaf, and is uniformly mixed evenly；（8）It rubs three times：Uniform tealeaves will be sprayed to be placed in rolling machine, adopted
With the mode of rubbing gently rubbed, 8min is gently rubbed；（9）Secondary fermentation：Tealeaves is gently fermented under closed environment again, is fermented
30 DEG C of room temperature, fermentation time 3h；（10）Drying：By the way of low temperature drying, by secondary fermentation leaf, first 60 DEG C of primary drying for baking dry 3h,
Again 80 DEG C of final firing Titian 20min to get；
Wherein, the preparation method of the edible mushroom complex polysaccharide liquid and edible mushroom composite enzyme liquid is as follows：1）Feedstock processing：It takes
Needle mushroom and mushroom, by the two amount ratio 3:1 mixing adds water mashing that edible mushroom composite pulp is made；2）Supersonic synergic enzymolysis processing：It will
Step 1）Edible mushroom composite pulp be placed in ultrasonic extractor and carry out ultrasonication, handled under the conditions of 600W, 40 DEG C
45min；0.1% is added in composite pulp that treated（w/w）Complex enzyme digests 2h under the conditions of 6,35 DEG C of pH, and heat up enzyme deactivation, obtains
To edible mushroom enzymolysis liquid, the complex enzyme is by flavor protease, cellulase and pectase in mass ratio 4:4:1 composition；3）
The preparation of complex polysaccharide liquid：Take step 2）Edible mushroom enzymolysis liquid, through centrifuge, take supernatant, concentration, be added ethyl alcohol stand, from
The heart collects precipitation, is crushed after vacuum freeze drying, and edible mushroom complex polysaccharide liquid is made after adding water dilution；4）Composite enzyme liquid
Preparation：Take step 2）Edible mushroom enzymolysis liquid, be added appropriate brown sugar, high-temperature sterilization after dissolving, inoculation 1%（w/w）Composite fermentation
Bacterium, is placed in 25 DEG C of insulating boxs the 30d that ferments, and zymotic fluid is crushed after vacuum freeze drying, obtained through centrifuging, taking supernatant, concentration
Edible mushroom composite enzyme, the composite zymocyte is by saccharomycete and Lactobacillus casei in mass ratio 3:1 composition.
Embodiment 2：
A kind of preparation method for the black tea that relieves the effect of alcohol, processing step（1）Extremely（10）It is most of same as Example 1, it differs only in：Food
With the step 1 of the preparation method of bacterium complex polysaccharide liquid and edible mushroom composite enzyme liquid）Middle feedstock processing, takes needle mushroom and mushroom,
By the two amount than 1:1 mixing.
Embodiment 3：
A kind of preparation method for the black tea that relieves the effect of alcohol, processing step（1）Extremely（10）It is most of same as Example 1, it differs only in：Food
With in the preparation method of bacterium complex polysaccharide liquid and edible mushroom composite enzyme liquid, step 1）Feedstock processing, take needle mushroom and mushroom,
By the two amount than 4:1 mixing
Comparative example A 1-3：
A kind of preparation method for the black tea that relieves the effect of alcohol, processing step（1）Extremely（10）It is most of same as Example 1, it differs only in：Food
With the step 1 of the preparation method of bacterium complex polysaccharide liquid and edible mushroom composite enzyme liquid）The original of middle feedstock processing, needle mushroom and mushroom
Material selection and proportioning are different（Specifically match such as table 1）.
Table 1
Comparative example B1-4：
A kind of preparation method for the black tea that relieves the effect of alcohol, processing step（1）Extremely（10）It is most of same as Example 1, it differs only in：Food
It is matched with the addition of bacterium complex polysaccharide liquid and edible mushroom composite enzyme liquid different（Specifically match such as table 2）.
Table 2
Experimental design：
Sober-up function possessed by the health protection tea of the present invention is confirmed by anti-intoxication experiment below.
Materials and methods：
Experimental animal：Healthy Kunming mouse is selected, weight is random to be grouped between 20-28g.
Material：According to the health-care black tea that embodiment 1-3 and comparative example are prepared, adds water to brew and be deployed into drink；Physiology
Brine；Red Star strong, colourless liquor distilled from sorghum white wine, alcohol content 56%；Alcohol dehydrogenase（ADH）Kit, glutamic-oxalacetic transaminease（AST）Kit,
Glutamic-pyruvic transaminase（ALT）Kit.
Method：
1, experiment packet is arranged：Mouse is taken respectively, is randomly divided into 4 big groups, is respectively set as embodiment group（Embodiment 1-3）, it is empty
White group（Physiological saline example）, comparative example A's group（Comparative example A 1-3）, comparative example B groups（Comparative example B1-3）, every example 5；What is prepared is red
The brewed physiological saline for being configured to uniform concentration 20g tealeaves/kg drinks and same concentrations of tea；It is 30g/kg to gavage dosage
Mouse.
2, drunk dosage determines：Take mouse 30, half male and half female is randomly divided into 6 groups, every group 5, each group after fasting 12h
Gavage gives the white wine 5-30ml/kg mouse of various dose respectively（5,10,15,20,25,30ml/kg）, observation mouse, which climbs, to disappear
Become homeless need capacity for liquor and it is drunk after various physiological changes, such as instability of gait, climbing areflexia, righting reflex loss, it is shallow sleep,
Abdominal respiration is apparent, death does not occur etc., so that it is determined that going out drunk dosage.Through mice drunk dosimetry, determine 15ml white wine/
Kg mouse are the drunk dosage of this experiment mice.
3, experimentation
（1）Anti-intoxication experiment：It takes mouse 50 and is grouped, after fasting 12h, first gavage gives embodiment group, blank group, comparison respectively
Example A groups, comparative example B groups give white wine after 30min by drunk dosage 15ml/kg gavages.Observe and record of each group mice drunk
Number, mouse to the time for righting reflex loss (occurring drunk) occur, comparing each group mice drunk situation after drinking.
（2）Biochemical Indexes：It takes mouse 50 and is grouped, after fasting 12h, first gavage gives embodiment group, sky respectively
White group, comparative example A's group, comparative example B groups give white wine after 30min by drunk dosage 15ml/kg gavages.To taking liver after wine 1h,
Measure alcohol dehydrogenase in liver（ADH）, glutamic-oxalacetic transaminease（AST）, glutamic-pyruvic transaminase（ALT）.
（3）It sobers up experiment：It takes mouse 50 and is grouped, after fasting 12h, first given by drunk dosage 15ml/kg gavages white
Wine, occurs after drunk reaction 5min that gavage gives corresponding embodiment group, blank group, comparative example A's group, comparative example B groups respectively, and opens
Beginning timing.Observe and record the time of the time and righting reflex recovery of each group mouse righting reflex loss.
Experimental result：
Embodiment is compared verification with comparative example.The results are shown in Table 3：
Table 3
Pass through the above comparison result, it is found that first, compared to blank group, black tea is first given before gavage white wine, can be shown
Writing reduces the drunk mouse number of mouse, i.e. the formula of embodiment 1-3 and comparative example A/B can reduce the drunk quantity of mouse, simultaneously
Also drunk incubation period is significantly extended, and it can be found that the drunk preclinical quantized data of 1-3 of the embodiment of the present invention is also wanted
It is substantially better than comparative example A 1-3 and comparative example B1-3, is had effects that more preferably anti-intoxication.Secondly, by sobering up, experimental result can be seen
Go out, the mouse of gavage embodiment 1-3 formula is smaller than blank group and comparative example group sobering up on the time, illustrate that the present invention has more
Good sobering-up functions.Furthermore, it is known that alcohol dehydrogenase（ADH）It is the key enzyme during alcohol metabolism, the height of vigor is determined
The speed of alcohol metabolism is determined, the more high then alcohol metabolism of vigor is faster, and also better for the effect relieved the effect of alcohol, millet straw turns ammonia in liver
Enzyme（AST）And glutamic-pyruvic transaminase（ALT）Activity be to judge the important indicator of alcohol-induced acute hepatic injury, activity get over Gao Ze
It is bigger to hepar damnification, and according to above-mentioned experimental data it is found that 1-3 formulas of the embodiment of the present invention are compared to blank group and comparison
Example, can significantly improve mouse ADH vigor, and reduce the activity of ALT, AST in mouse liver, show that alcohol metabolism can be improved in it
Speed reduces the bioavilability of ethyl alcohol in vivo, to have certain antialcoholism action, is damaged simultaneously for acute alcoholic liver
Wound has significant protective effect.
To sum up, by above-mentioned data and analysis it can be confirmed that the black tea that relieves the effect of alcohol that the present invention is prepared, eats before drinking
With with good anti-intoxication effect, while compared to the exclusive use and other combination matchings for using edible fungi raw materials, Yi Jixiang
Than in by the exclusive use of polysaccharide obtained, ferment and other combination matchings, formula of the invention produces synergistic
Effect, reach it is anti-intoxication, sober up and liver protection and other effects, have exceeded the expection of those skilled in the art.
Claims (10)
1. a kind of preparation method for the black tea that relieves the effect of alcohol, which is characterized in that preparation process is：Leaf picking withers, once rubs, spraying
Spill edible mushroom complex polysaccharide liquid, it is secondary rub, one time fermentation, sprinkling edible mushroom composite enzyme liquid, rub three times, secondary fermentation,
Baking step.
2. the preparation method of the black tea according to claim 1 that relieves the effect of alcohol, which is characterized in that
（1）Leaf picking：Fresh leaves of tea plant is picked as raw material；
（2）It withers：It is 60-75% fresh leaf uniformly to be spread on withering trough, withering to fresh leaf moisture content；
（3）Once rub：Withering leaf is placed in rolling machine, rubbed with pneumatics-light kneadding twirl-pneumatics rubs-weight rubs
Sequentially, carry out rubbing operation successively, until tea juice start it is excessive, preliminarily form strip；
（4）Spray edible mushroom complex polysaccharide liquid：Withering leaf is placed in blender, under stirring, complex polysaccharide liquid is pressed
According to 200-500mL/kg tealeaves ratio uniform be sprayed on withering leaf, and be uniformly mixed；
（5）It is secondary to rub：Uniform tealeaves will be sprayed to be placed in rolling machine, using the mode of rubbing gently rubbed, gently rub 10-20min；
（6）One time fermentation：Tealeaves is gently fermented under closed environment, 25-35 DEG C of room temperature of fermentation, fermentation time 5-10h；
（7）Spray edible mushroom composite enzyme liquid：One time fermentation leaf is placed in blender, under stirring, by composite enzyme
Liquid according to 50-100mL/kg tealeaves ratio uniform be sprayed on one time fermentation leaf, and be uniformly mixed；
（8）It rubs three times：Uniform tealeaves will be sprayed to be placed in rolling machine, using the mode of rubbing gently rubbed, gently rub 5-10min；
（9）Secondary fermentation：Tealeaves is gently fermented under closed environment again, 25-35 DEG C of room temperature of fermentation, fermentation time
0.5-5h；
（10）Drying：By the way of low temperature drying, 50-80 DEG C of primary drying for baking of secondary fermentation leaf elder generation is dried into 0.5-3h, then 60-90 DEG C
Final firing Titian 5-30min to get；
Wherein, the preparation method of the edible mushroom complex polysaccharide liquid and edible mushroom composite enzyme liquid is as follows：1）Feedstock processing：It takes
Needle mushroom and mushroom, by the two amount ratio（1-4）:1 mixing adds water mashing that edible mushroom composite pulp is made；2）At supersonic synergic enzymolysis
Reason：By step 1）Edible mushroom composite pulp be placed in ultrasonic extractor and carry out ultrasonication, in composite pulp after treatment
Complex enzyme zymohydrolysis is added, heat up enzyme deactivation, obtains edible mushroom enzymolysis liquid；3）The preparation of complex polysaccharide liquid：Take step 2）Edible mushroom
Edible mushroom complex polysaccharide liquid is made after extracted complex polysaccharide for enzymolysis liquid；4）The preparation of composite enzyme liquid：Take step 2）It is edible
Edible mushroom composite enzyme liquid is made through inoculation fermentation in bacterium enzymolysis liquid.
3. the preparation method of the black tea according to claim 2 that relieves the effect of alcohol, which is characterized in that the complex polysaccharide liquid and compound
The preparation method of enzyme liquid is as follows：
1）Feedstock processing：Needle mushroom and mushroom are taken, by the two amount ratio（1-4）:1 mixing adds water mashing that edible mushroom composite pulp is made；
2）Supersonic synergic enzymolysis processing：By step 1）Edible mushroom composite pulp be placed in ultrasonic extractor carry out ultrasonic wave at
Reason, 30-60min is handled under the conditions of 300-800W, 30-50 DEG C；0.05-0.2% is added in composite pulp that treated（w/w）It is multiple
Synthase digests 1-3h under the conditions of pH 5-7,30-50 DEG C, and heat up enzyme deactivation, obtains edible mushroom enzymolysis liquid；
3）The preparation of complex polysaccharide liquid：Take step 2）Edible mushroom enzymolysis liquid, through centrifuge, take supernatant, concentration, be added ethyl alcohol it is quiet
It sets, precipitation is collected by centrifugation, is crushed after vacuum freeze drying, edible mushroom complex polysaccharide liquid is made after adding water dilution；
4）The preparation of composite enzyme liquid：Take step 2）Edible mushroom enzymolysis liquid, appropriate brown sugar is added, high-temperature sterilization after dissolving connects
Kind composite zymocyte, is placed in insulating box and ferments, and it is compound that edible mushroom is made through centrifuging, taking supernatant, after being diluted with water for zymotic fluid
Enzyme liquid.
4. the preparation method of the black tea according to claim 3 that relieves the effect of alcohol, which is characterized in that step 1）In, by needle mushroom and perfume (or spice)
Mushroom mass ratio 3:1 mixing adds water mashing that edible mushroom composite pulp is made.
5. the preparation method of the black tea that relieves the effect of alcohol according to claim 1-4, which is characterized in that step 2）In, the ultrasound
Coordinated enzymatic hydrolysis processing, concrete operations are：By step 1）Edible mushroom composite pulp be placed in ultrasonic extractor carry out ultrasonic wave at
Reason, 45min is handled under the conditions of 600W, 40 DEG C；0.1% is added in composite pulp that treated（w/w）Complex enzyme, in 6,35 DEG C of pH
Under the conditions of digest 2h, heat up enzyme deactivation, obtains edible mushroom enzymolysis liquid.
6. the preparation method of the black tea according to claim 5 that relieves the effect of alcohol, which is characterized in that step 2）In, the complex enzyme
In mass ratio by flavor protease, cellulase and pectase（2-5）:（2-5）:1 composition.
7. the preparation method of the black tea according to claim 6 that relieves the effect of alcohol, which is characterized in that step 2）In, the complex enzyme
By flavor protease, cellulase and pectase in mass ratio 4:4:1 composition.
8. the preparation method of the black tea that relieves the effect of alcohol according to claim 1-4, which is characterized in that step 4）In, take step 2）'s
Edible mushroom enzymolysis liquid, is added appropriate brown sugar, and high-temperature sterilization after dissolving is inoculated with 0.5-1%（w/w）Composite zymocyte is placed in 20-30
Ferment 20-40d in DEG C insulating box, and zymotic fluid crushes after vacuum freeze drying through centrifuging, taking supernatant, concentration, obtains edible mushroom
Composite enzyme.
9. the preparation method of the black tea according to claim 8 that relieves the effect of alcohol, which is characterized in that step 4）In, the compound hair
Yeast-like fungi by saccharomycete and Lactobacillus casei in mass ratio（3-5）:1 composition.
What 10. the preparation method according to claim 1-9 obtained relieve the effect of alcohol black tea.
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