CN108732342A - A kind of assessment detection kit of fecundity - Google Patents
A kind of assessment detection kit of fecundity Download PDFInfo
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- CN108732342A CN108732342A CN201710259691.0A CN201710259691A CN108732342A CN 108732342 A CN108732342 A CN 108732342A CN 201710259691 A CN201710259691 A CN 201710259691A CN 108732342 A CN108732342 A CN 108732342A
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- G—PHYSICS
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- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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Abstract
The present invention relates to a kind of assessment detection kits of fecundity, including AMH and INH B detector bars, the detector bar is equipped with bonding pad and chromatographic film, has the immune labeled antibody of AMH and the immune labeled antibody of INH B on bonding pad, and AMH detection antibody and INH B detection antibody are coated in chromatographic film.The technical program can diagnose AMH and INH B simultaneously by primary sample, using simplicity, and further increase the accuracy of reproductive function detection, and testing result provides strong foundation for accurate treatment in next step, is suitable for large-scale promotion application.
Description
Technical field
The invention patent relates to detection kit technical fields, and in particular to detection kit technical field, more particularly to
A kind of assessment detection kit of fecundity.
Background technology
Women's fecundity and age, basic follicle stimulating hormone, inhibin B（Inhibin B, INH B）, anti-Miao Le Shi pipe
Hormone（Anti mullerian hormone, AMH）, estradiol, many factors such as Antral follicles level it is related, wherein AMH and
INH B are mostly important two indices.
AMH belongs to β-transforming factor by the single-stranded glycoprotein dimers formed of two 72kD that disulphide bridges connect
A member of family；AMH is secreted by the granular cell of ovarian growth ovarian follicle after the immature Sertoli cells of testis and birth, tool
Have and adjusts cell development and differentiation, the function of making male embryo Miao's Le Shi pipes degenerate.For women, AMH is in 36 week foetal period
Follicular cell generate, until Menopause, AMH is gradually decreased down can not detection level.AMH is clinically mainly used
In Ovary reserve, prediction ovary responsiveness, male reproductive function assessment etc..
INH B belong to β-transforming factor family, can monitor gonad function, draw in male sterility and female ovary function
The female acyesis risen is of great significance.
As described above, AMH and INH B are clinically mainly for assessment of the reproductive function of male and female, guidance is clinical
Treatment.
Traditional AMH and INH B detection methods include enzyme linked immunosorbent assay, chemoluminescence method, are essentially all to rely on
Large-scale instrument and equipment；But INH B and AMH are close associations, but relatively independent two indices；Existing detection reagent
Box, is two independent products, can all be had differences from sample process, loading etc., to cause shadow to ratio between the two
It rings.
In order to solve the existing above problem, it is desirable to provide a kind of kit can simultaneously be detected by primary sample
AMH and INH B, it would be possible to influence factor be reduced to minimum, fecundity is detected, it is easy to use.
Invention content
The purpose of the present invention is overcome it is above-mentioned in the prior art the shortcomings that, a kind of assessment detection examination of fecundity is provided
The assessment detection kit of agent box, the fecundity can detect AMH and INH B simultaneously by primary sample, using simplicity, and
The accuracy of reproductive function detection is further increased, testing result provides strong foundation for accurate treatment in next step, is suitable for big
Scale promotes and applies.
To achieve the goals above, a kind of assessment detection kit of fecundity, including AMH detector bars and INH are provided
B detector bars）, the detection kit includes AMH detector bars and INH B detector bars, the detector bar be equipped with bonding pad and
Chromatographic film has the immune labeled antibody of AMH or the immune labeled antibody of INH B on bonding pad, and it is anti-that AMH detections are coated in chromatographic film
Body or INH B detect antibody.Wherein, the chromatographic film can be nitrocellulose filter, cellulose acetate film, nylon membrane etc..
As is well known to those skilled in the art, detector bar also referred to as detects card or other titles.
More preferably, have the immune labeled antibody of AMH and INH B immune on the bonding pad of the AMH and INH B detector bars
Labelled antibody is coated with AMH detection antibody and INH B detection antibody in the chromatographic film of the AMH and INH B detector bars.
More preferably, there is the immune labeled antibody of AMH, the chromatography of the AMH detector bars on the bonding pad of the AMH detector bars
It is coated with AMH detection antibody on film, there is the immune labeled antibody of INH B, the INH on the bonding pad of the INH B detector bars
INH B detection antibody is coated in the chromatographic film of B detector bars.
Further, the AMH detector bars and the INH B detector bars are set up in parallel.
Further, the assessment detection kit of the fecundity further includes bottom plate, AMH detector bars and described
INH B detector bars are fitted in respectively on the upper and lower surface of the bottom plate.
Further, detection line and nature controlling line are disposed between bonding pad and chromatographic film on the detector bar,
Wherein detection line is coated with anti-AMH antibody or anti-INH B antibody；Between bonding pad and detection line, the separate nature controlling line of chromatographic film
Side is respectively equipped with protective film.Further, the chromatographic film is nitrocellulose filter, cellulose acetate film or nylon
Film.Further, the protective film is the opaque protective film in part.
More preferably, the immune labeled antibody of the AMH is the immune labeled antibody of anti-AMH, and the AMH detections antibody is anti-AMH
Antibody, the immune labeled antibody of INH B are the immune labeled antibody of INH B, and the INH B detections antibody is INH B antibody.
More preferably, the immune labeled antibody of the AMH is AMH fluorescent labeled antibodies or AMH latexes labelled antibody or AMH colloids
Golden labelled antibody, the immune labeled antibody of INH B are INH B fluorescent labeled antibodies or INH B latexes labelled antibodies or INH B
Colloidal gold labeled monoclonal antibody
Beneficial effects of the present invention mainly have：
（1）Including AMH detector bars and INH B detector bars, to primary sample, you can be detected to AMH and INH B；It avoids
Sampling causes patient uncomfortable repeatedly, simplifies detection operation；
（2）AMH and INH B are detected simultaneously, sample-adding amount, sample disposal between detecting etc. are highly consistent, relative to two indices point
Open detection, accuracy higher.
Therefore, the present invention can detect AMH and INH B simultaneously by primary sample, using simplicity, and further increase life
The accuracy of Function detection is grown, testing result provides strong foundation for accurate treatment in next step, is suitable for large-scale promotion application.
Description of the drawings
Fig. 1 is the structural schematic diagram of a specific embodiment of the technical program；
Fig. 2 is the structural schematic diagram of the another specific embodiment of the technical program；
Fig. 3 is the structural schematic diagram of the still another embodiment of the technical program.
Part reference numeral：1- bonding pads, 2- bonding pads, 3- protective films, the first detection lines of 4-, the second detection lines of 5-, 6-
Nature controlling line, 7- nitrocellulose filters, 8- protective films；12- bonding pads, 22- bonding pads, 32- protective films, the first detection lines of 42-,
The second detection lines of 52-, 62- nature controlling lines；72- nitrocellulose filters, 82- protective films, 9- bottom plates.
Specific implementation mode
In order to be more clearly understood that the technology contents of the present invention, spy are described in detail in conjunction with specific embodiments.
1 fecundity of embodiment assesses test agent structure and detection method
A kind of assessment detection kit 10 of fecundity as shown in Figure 1, detection kit include AMH and INH B detections
Item 90 is placed with bonding pad on the detector bar 90 successively（Containing anti-AMH fluorescent labeled antibodies）1, bonding pad（It is glimmering containing anti-INH B
Signal antibody）2, the opaque protective film 3 in part, the first detection line（It is coated with anti-AMH antibody）4, the second detection line（Coating is anti-
INH B antibody）5, nature controlling line 6, nitrocellulose filter 7 and opaque protective film 8.Have AMH immune labeled anti-i.e. on bonding pad
Body and INH B immune labeled antibody are coated with AMH detection antibody and INH B detection antibody in chromatographic film, detect simultaneously respectively
AMH and INH B.
When specific detection, fluorescence immunity analyzer is opened（The fluorescence immunoassay of the Guangzhou bio tech ltd Lan Bo production
Analyzer AFS-1000）, check whether the lot number of calibration card and kit is consistent, then in calibration card inserting instrument and high-ranking officers
Curve in quasi- card imports instrument；Balance is taken out to the detector bar after room temperature, sample is drawn and is added drop-wise in the well of detector bar
（80 μ L of serum sample）, react at room temperature 15 minutes；Detector bar is inserted into holding for fluorescence immunity analyzer by typing sample relevant information
It carries in device, by feeler switch, instrument will automatically be scanned detector bar；The automatic testing result of fluorescence immunity analyzer simultaneously calculates sample
The content of AMH and INH B in this；Used detector bar is taken out, endangers product by potential source biomolecule to handle.
2 fecundity of embodiment assesses test agent structure and detection method
A kind of assessment detection kit 10 of fecundity as shown in Figure 2, detection kit include upper and lower two rows side by side
The AMH detector bars 91 and INH B detector bars 92 of cloth, the detector bar 91,92 are equipped with bonding pad 12,22, the opaque guarantor in part
Cuticula 32, the first detection line（It is coated with anti-AMH antibody）42, the second detection line（It is coated with anti-INH B antibody）52, nature controlling line 62, and
Nitrocellulose filter 72 and opaque protective film 82.Have the immune labeled antibody of AMH and INH B immune labeled anti-i.e. on bonding pad
Body is coated with AMH detection antibody and INH B detection antibody in chromatographic film；The AMH detector bars and INH B detector bars, put side by side
Enter to detect in cartridge, sample is added in AMH detector bars and the corresponding well of INH B detector bars, detects simultaneously respectively
AMH and INH B.
When specific detection, fluorescence immunity analyzer is opened（The fluorescence immunoassay of the Guangzhou bio tech ltd Lan Bo production
Analyzer AFS-1000）, check whether the lot number of calibration card and kit is consistent, then in calibration card inserting instrument and high-ranking officers
Curve in quasi- card imports instrument；Balance is taken out to the detector bar after room temperature, sample is drawn and is added drop-wise to AMH detector bars and INH B
In the corresponding well of detector bar（80 μ L of serum sample are added in each well）, react at room temperature 15 minutes；Typing sample
Detector bar is inserted into the carrier of fluorescence immunity analyzer by relevant information, and by feeler switch, instrument will automatically carry out detector bar
Scanning；The automatic testing result of fluorescence immunity analyzer and the content for calculating AMH and INH B in sample；Used detector bar is taken out,
Product are endangered by potential source biomolecule to handle.
3 fecundity of embodiment assesses test agent structure and detection method
A kind of assessment detection kit 10 of fecundity as shown in Figure 3, detection kit 10 include 93 He of AMH detector bars
INH B detector bars 94 differentiate the upper and lower surface pasted in bottom plate 9, and the detector bar is equipped with bonding pad 13,23, and part is opaque
Protective film 33, the first detection line（It is coated with anti-AMH antibody）43, the second detection line（It is coated with anti-INH B antibody）53, nature controlling line 63,
Nitrocellulose filter 73 and opaque protective film 83.Have the immune labeled antibody of AMH and INH B immune labeled anti-i.e. on bonding pad
Body is coated with AMH detection antibody and INH B detection antibody in chromatographic film；The AMH detector bars and INH B detector bars, glue respectively
The upper and lower surface of patch and bottom plate, is added sample in AMH detector bars and the corresponding well of INH B detector bars, can distinguish
Detect AMH and INH B.
When specific detection, fluorescence immunity analyzer is opened（The fluorescence immunoassay of the Guangzhou bio tech ltd Lan Bo production
Analyzer AFS-1000）, check whether the lot number of calibration card and kit is consistent, then in calibration card inserting instrument and high-ranking officers
Curve in quasi- card imports instrument；Balance is taken out to the detector bar after room temperature, draws the sample-adding that sample is first added drop-wise to AMH detector bars
Kong Zhong；Overturning detection card, sample is added in the corresponding well of INH B detector bars after 1 minute（Serum is added in each well
80 μ L of sample）, react at room temperature 15 minutes；Detector bar is inserted into the carrier of fluorescence immunity analyzer by typing sample relevant information
In, by feeler switch, instrument will automatically be scanned detector bar（AMH detector bars are first scanned, then scan INH B detections again
Item）；The automatic testing result of fluorescence immunity analyzer and the content for calculating AMH and INH B in sample；Used detector bar is taken out,
Product are endangered by potential source biomolecule to handle.
Embodiment 4 detects the preparation of box
（1）Fluorescent marker
Cleaning：Take fluorescent microsphere（Bangs Lab, article No.：11233）Into centrifuge tube, add 0.1M MES（pH 5.0）Buffer solution
Mixing, and with 13000rpm, 15min, 4 DEG C of pelleted by centrifugation, supernatant is abandoned, with 0.1M MES（pH 5.0）Buffer solution is resuspended for use.
It activates and cleans：By activator EDC, NHS and fluorescent microsphere according to mass ratio ratio 2:1:2 amount is activated, and concrete operations are such as
Under：
It weighs EDC, NHS and 0.1M MES is added（pH 5.0）It is dissolved in buffer solution, rapidly takes and to be finished in right amount to 4.3.3.1 cleanings
In fluorescent microsphere, is sealed with sealed membrane and be placed on room temperature on 200rpm shaking tables and shake up 30min, 13000rpm, 30min, 4 after taking-up
DEG C it is centrifuged off supernatant, with equivalent 0.1M MES（pH 6.5）Buffer solution is resuspended ultrasonic mixing and cleans, and is repeated more than once behaviour
Make i.e. cleaning twice.It is for use that supernatant is discarded after the completion of centrifugation.
Label：Latex after activation is resuspended to 0.1M MES（pH 6.5）In buffer solution, it is separately added into antibody PG- rapidly
01 and FG-01, mixing are placed room temperature, are shaken up 4 hours on 200rpm shaking tables.
Closing：The microballoon for taking above-mentioned label good is centrifuged off supernatant in 13000rpm, 30min, 4 DEG C, immediately into microballoon
Equivalent confining liquid is added, ultrasound is placed room temperature after being resuspended, shaken up on 200rpm shaking tables 1 hour again.13000rpm after the completion of reaction,
20min, 4 DEG C of centrifugations, takes supernatant to be checked.
& is cleaned to be resuspended：Re-suspension liquid is added, ultrasound piping and druming is resuspended, with high speed freezing centrifuge 13000rpm, 20min, 4 DEG C
Centrifugation, discards supernatant；Re-suspension liquid is added, ultrasound piping and druming is resuspended.
The microspheres solution being resuspended marks, spare.
（2）The specking of bonding pad
Antibody for AMH diagnosis（Anti-human AMH）The specking method of bonding pad is as follows：Fluorescent labeled antibody solution dilutes：With glimmering
The fluorescent labeled antibody conjugate of above-mentioned preparation is diluted 4 times by signal antibody re-suspension liquid；Point film instrument is set, point film instrument is opened
Power supply, sets specking program, and specking amount is 8 μ l/cm；No. 1 pipeline is specking channel；Point film instrument initializes：No. 1 pipeline is set
It is resuspended in solution in fluorescent labeled antibody, selects initialization program, initialize 6 cycles；Specking：Bonding pad is pressed into fixed position
It lies in point film instrument, presses on control panel " GO " key and start specking, removed after having put, check the good bonding pad of specking, specking
Fluorescent labeled antibody band uniformly, continuous and the entire bonding pad of perforation straight line be qualified specking product, occur in two straight lines
Breakpoint is unqualified specking product；A piece of bonding pad is often put, it is that specking is primary to press " GO " key on a control panel（It is a piece of）；Spray
Point terminates, and the bonding pad of specking is placed in room temperature and is spontaneously dried 1 hour, specking trace should be can't see on film.
Antibody bonding pad specking method for INH B diagnosis is same as above.
（3）Chromatographic film（AMH+INH B）Preparation
Antibody for AMH and INH B diagnosis（Anti-human AMH）It is as follows to chromatograph membrane preparation method：Take the anti-AMH polyclonal antibodies of chicken
The 500 μ g and anti-500 μ g of INH B polyclonal antibodies of mouse, are added separately in 5ml graduated centrifuge tubes, antibody diluent to 1ml, container
Label T1, T2 marks respectively.25 μ l mixing of 25 μ l of sheep anti-mouse igg antibody and goat-anti chicken antibody is taken, 5ml graduated centrifuge tubes are added to
In, antibody diluent to 1ml, Container Tag C marks.Point film instrument is set, the power supply of point film instrument is opened, sets specking program, spray
Point amount is 1 μ l/cm；No. 1 pipeline is detection band T1 speckings channel, and No. 2 pipelines are detection band T2 speckings channel, and No. 3 pipelines are pair
According to band specking channel；Point film instrument initializes：No. 1 pipeline is placed in AMH antibody-solutions, it is molten that No. 2 pipelines are placed in IHN B antibody
In liquid, No. 3 pipelines are placed in control band solution, initialization program is selected, initialize 6 cycles；Specking：By chromatographic film by solid
Positioning horizontalization is placed in point film instrument, is pressed on control panel " GO " key and is started specking, is removed after having put, and checks the good chromatography of specking
Film, detection band and control band for two uniformly, continuous and the entire chromatographic film of perforation straight lines be qualified specking product, in three straight lines
Appearance breakpoint is unqualified specking product；A piece of chromatographic film is often put, it is that specking is primary to press " GO " key on a control panel（One
Piece）；Specking terminates, and the chromatographic film of specking is placed in room temperature and is spontaneously dried 1 hour, specking trace should be can't see on film.
（4）The preparation of chromatographic film
Antibody for AMH diagnosis（Anti-human AMH）It is as follows to chromatograph membrane preparation method：The anti-500 μ g of AMH polyclonal antibodies of chicken are taken, are added
Into 5ml graduated centrifuge tubes, antibody diluent to 1ml, Container Tag T flag.25 μ l of sheep anti-mouse igg antibody are taken, 5ml is added to
In graduated centrifuge tube, antibody diluent to 1ml, Container Tag C marks.Point film instrument is set, the power supply of point film instrument, setting spray are opened
Point program, specking amount are 1 μ l/cm；No. 1 pipeline is detection band specking channel, and No. 2 pipelines are control band specking channel；Point film instrument
Initialization：No. 1 pipeline is placed in detection band solution, No. 2 pipelines are placed in control band solution, select initialization program, just
6 cycles of beginningization；Specking：Chromatographic film is lain in by fixed position in point film instrument, " GO " key is pressed on control panel and starts specking,
It is removed after having put, checks the good chromatographic film of specking, detected band and control band is uniform for two, continuous and the entire chromatographic film of perforation
Straight line is qualified specking product, and it is unqualified specking product to occur breakpoint in two straight lines；A piece of chromatographic film is often put, by a control plane
" GO " key on plate is that specking is primary（It is a piece of）；Specking terminates, and the chromatographic film of specking is placed in room temperature and is spontaneously dried 1 hour,
Specking trace should be can't see on film.
Chromatography membrane preparation method for INH B diagnosis is same as above.
（5）Pad pasting cuts film
The protection sheet of wider portion on bottom plate is removed, along the lower edge of protection sheet above, the chromatographic film of line will be pulled, with C lines
Mode above is attached on bottom plate plate；Bonding pad is attached to below T lines, is contacted a little with NC films；Sample pad is attached to combination
Pad lower section, contacts a little with bonding pad；Then top protection sheet is removed, blotting paper is attached to the top of NC films, it is a little with NC films
Contact；Protection sheet and instruction band paper are attached to one by one outside assembled test strips, kilocalorie is assembled into.
Cutting electromechanical source is connected, setting cuts film program, sets cutting width as 4mm；Kilocalorie certified products are kept flat into cutting
In machine platform track, face-up, " GO " key is pressed on operation panel, starts to cut；A piece of kilocalorie certified products are often put, by operation
" GO " key is primary on panel, until having cut all kilocalorie certified products；After the completion of cutting, it will be used to detect AMH and INH respectively
The test strips of B stick on bottom plate, composition AMH and INH B test strips.
（6）Kit assembles
Above-mentioned test strips are fitted into cartridge, detector bar is formed.
Take aluminium foil bag and drier；Open heat sealing machine, preheating；Detector bar to be packed, 1 bag of drier are packed into aluminium foil bag
In；According to aluminium foil bag of the length cut-out equipped with detector bar and drier of regulation；Aluminium foil bag is sealed with heat sealing machine；It is labelled.
5 clinical detection recruitment evaluation of embodiment
By the detector bar of above-described embodiment 2, verified with clinical sample.
Sample to be tested is added drop-wise in the sample aperture of detector bar, is subsequently placed in fluorescence detector and is read.
Testing result and Follow-up results（To normal ovulation after self-test）It is as follows：
| Contrast agents box：It is positive | Contrast agents box：It is negative | It is total | |
| Detection is positive | 20 | 11 | 31 |
| Detection is negative | 7 | 121 | 128 |
| It is total | 27 | 132 | 159 |
The detection sensitivity of the test strips is：20/（20+7）× 100%=74.07%；
The detection specificity of the test strips is：121/（121+11）=91.67%.
Above-described embodiment meets clinical application the result shows that the accuracy rate of this detector bar is higher.
6 clinical detection recruitment evaluation of embodiment
By the detector bar of above-described embodiment 1, verified with clinical sample.
Sample to be tested is added drop-wise in the sample aperture of detector bar, is subsequently placed in fluorescence detector and is read.
Testing result and Follow-up results（To normal ovulation after self-test）It is as follows：
| Contrast agents box：It is positive | Contrast agents box：It is negative | It is total | |
| Detection is positive | 25 | 4 | 29 |
| Detection is negative | 2 | 128 | 130 |
| It is total | 27 | 132 | 159 |
The detection sensitivity of the test strips is：25/（25+2）× 100%=92.59%；
The detection specificity of the test strips is：128/（128+4）=96.97%.
Above-described embodiment meets clinical application the result shows that the accuracy rate of this detector bar is very high.
Kit made from the present embodiment can not only be detected AMH and INH B by primary sample, avoid
Sample detection causes the interference that result is inaccurate and environment is to testing result repeatedly, by detecting AMH and INH B two simultaneously
A index improves the accuracy of reproductive function detection, and can be according to the testing result of AMH and INH B two indices, into the hand-manipulating of needle
To the stronger therapeutic scheme of property.
Therefore, the present invention can diagnose AMH and INH B simultaneously by primary sample, using simplicity, and further increase life
The accuracy of Function detection is grown, testing result provides strong foundation for accurate treatment in next step, is suitable for large-scale promotion application.
In this description, the present invention is described with reference to its specific embodiment.But it is clear that can still make
Spirit and scope of the various modifications and alterations without departing from patent of the present invention.Therefore, the description and the appended drawings should be considered as explanation
Property and not restrictive.
Claims (10)
1. a kind of assessment detection kit of fecundity, which is characterized in that the detection kit includes AMH and INH B
Detector bar, the detector bar are equipped with bonding pad and chromatographic film, have the immune labeled antibody of AMH and INH B wherein on bonding pad
Immune labeled antibody is coated with AMH detection antibody and INH B detection antibody in chromatographic film.
2. the assessment detection kit of fecundity according to claim 1, which is characterized in that AMH the and INH B
There is the immune labeled antibody of AMH and the immune labeled antibody of INH B on the bonding pad of detector bar, AMH the and INH B detector bars
AMH detection antibody and INH B detection antibody are coated in chromatographic film.
3. the assessment detection kit of fecundity according to claim 1, which is characterized in that the AMH detector bars
There is the immune labeled antibody of AMH on bonding pad, AMH detection antibody, the INH are coated in the chromatographic film of the AMH detector bars
There is the immune labeled antibody of INH B on the bonding pad of B detector bars, INH B inspections are coated in the chromatographic film of the INH B detector bars
Survey antibody.
4. the assessment detection kit of fecundity according to claim 3, which is characterized in that the AMH detector bars and
The INH B detector bars are set up in parallel.
5. the assessment detection kit of fecundity according to claim 3, which is characterized in that the fecundity is commented
It further includes bottom plate to estimate detection kit, the AMH detector bars and the INH B detector bars be fitted in respectively the bottom plate it is upper,
On lower surface.
6. according to the assessment detection kit of claim 1-5 any one of them fecundities, which is characterized in that the inspection
Survey on item and be disposed with detection line and nature controlling line between bonding pad and chromatographic film, wherein detection line be coated with anti-AMH antibody or
Anti- INH B antibody；Between bonding pad and detection line, the side of the separate nature controlling line of chromatographic film be respectively equipped with protective film.
7. the assessment detection kit of fecundity according to claim 6, which is characterized in that the chromatographic film is nitre
Acid cellulose film, cellulose acetate film or nylon membrane.
8. the assessment detection kit of fecundity according to claim 6, which is characterized in that the protective film is portion
Divide opaque protective film.
9. the assessment detection kit of fecundity according to claim 6, which is characterized in that the AMH is immune labeled
Antibody is the immune labeled antibody of anti-AMH, and the AMH detections antibody is anti-AMH antibody, and the immune labeled antibody of INH B is INH
The immune labeled antibody of B, the INH B detections antibody is INH B antibody.
10. the assessment detection kit of fecundity according to claim 6, which is characterized in that the AMH is immune labeled
Antibody is AMH fluorescent labeled antibodies or AMH latexes labelled antibody or AMH colloidal gold labeled monoclonal antibodies, and the INH B are immune labeled anti-
Body is INH B fluorescent labeled antibodies or INH B latexes labelled antibodies or INH B colloidal gold labeled monoclonal antibodies.
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| CN201710259691.0A CN108732342A (en) | 2017-04-20 | 2017-04-20 | A kind of assessment detection kit of fecundity |
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| CN201710259691.0A CN108732342A (en) | 2017-04-20 | 2017-04-20 | A kind of assessment detection kit of fecundity |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110275019A (en) * | 2019-08-01 | 2019-09-24 | 郑州迈迪迅医疗科技有限公司 | A kind of novel chromatography detection kit |
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2017
- 2017-04-20 CN CN201710259691.0A patent/CN108732342A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110275019A (en) * | 2019-08-01 | 2019-09-24 | 郑州迈迪迅医疗科技有限公司 | A kind of novel chromatography detection kit |
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