CN108732291A - The method for detecting maltopentaose using ultra performance liquid chromatography-mass spectrometric hyphenated technique - Google Patents
The method for detecting maltopentaose using ultra performance liquid chromatography-mass spectrometric hyphenated technique Download PDFInfo
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Abstract
The invention discloses a kind of methods detecting maltopentaose using ultra performance liquid chromatography-mass spectrometric hyphenated technique, include the following steps：（1）It takes maltopentaose appropriate, is dissolved in water, be configured to certain density test solution.（2）Sample introduction is detected by following chromatographic condition and Mass Spectrometry Conditions；Chromatographic condition is：Chromatographic column：Waters Xbridge BEH Amide 2.1x100mm；Mobile phase：Acetonitrile-water gradient, 0~15min keep 87% acetonitrile, 15~20min to gradually decrease down 15% acetonitrile by 87% acetonitrile, and 20~30min keeps 15% acetonitrile；Mass Spectrometry Conditions are：The sources ESI, Salbutamol Selected Ion Monitoring（SIM）Pattern, fragmentation voltage：130v, sheath gas：12L/min, capillary voltage：4000v, extraction accurate mass number [M-H]‑827.4, carry out linear quantitative with the common logarithm of concentration and peak area.This method have the characteristics that it is qualitative precisely, it is high sensitivity, precision height, good linearity, easy to operate, the analysis for being suitable for maltopentaose detects.
Description
Technical field
The present invention relates to a kind of methods detecting maltopentaose using ultra performance liquid chromatography-mass spectrometric hyphenated technique, belong to
Chemical analysis and detection field.
Background technology
Maltopentaose is a kind of oligosaccharide, and sugariness is relatively low, has inhibiting effect to spoilage organisms in enteron aisle, is widely used in eating
Product, cosmetics, medicine and other fields.Honey is apidae insect apis cerana Apis cerana Fabricius or apis mellifera Linnaeus
The honey that Apis mellifera Linnaeus are made.In recent years, various cheap fructose syrups, wheat are mixed in natural honey
The case where doping honey such as bud syrup, is extremely serious, and with rich amyloid plant, the hydrolysis sugars such as fructose syrup are produced by hydrolysis
Slurry hydrolyzes incomplete oligosaccharide ingredient, such as maltopentaose and the higher saccharide compound of molecular weight because hydrolysis syrup class can exist；
And without containing oligosaccharides more than pentasaccharides and molecular weight higher in natural honey, therefore the analysis detection side by studying maltopentaose
Method can provide new thinking for the anti-fake check of honey.
Maltopentaose, molecular formula are：C30H52O26, molecular weight 828.7, No. CAS is 34620-76-3, and structure is as schemed：
。
The method for being usually used in carbohydrate analysis at present mainly has ion-exchange chromatography（IEC）, exclusion chromatography（GPC）, it is high
Effect liquid phase chromatogram method（HPLC）, high performance capillary electrophoresis（HPCE）；Measure carbohydrate main detector be UV detector,
Infrared detector, electrochemical detector, differential refraction detector and evaporative light scattering detector.Due to glucide boiling point is high,
The features such as hardly possible volatilizees, is not easy to gasify, it is necessary to be detected by derivatization reaction once or twice, actual analysis is brought in this way
Error and inconvenience.The component of malto-oligosaccharide is determined by high performance liquid chromatography-differential refraction detector in capital Wu Hong et al.
Property and it is quantitative, though this method process is simple, need to be loaded product overlay method to six kinds of sugar in sample with time window method and standard specimen
Carry out qualitative, and bimodal, the right difference of Linear Quasi is presented in maltopentaose, and detection limit is too high, and sensitivity is low, to qualitative and quantitatively bring
Interference.It is therefore desirable to explore the detection method of fast qualitative and accurate quantitative analysis.
Invention content
Technical problem to be solved by the present invention lies in provide a kind of short operating time, high sensitivity, reproducible, line
Property the high maltopentaose detection method of content of degree of fitting, while for saccharide compound analysis detection new thinking is provided.
In order to solve the above technical problems, provided by the invention detect wheat using ultra performance liquid chromatography-mass spectrometric hyphenated technique
The method of bud pentasaccharides can be realized through the following steps：
（1）It takes maltopentaose appropriate, is dissolved in water, be configured to certain density test solution；
（2）Sample introduction is detected by following chromatographic condition and Mass Spectrometry Conditions；
Chromatographic condition is：Chromatographic column：Waters Xbridge BEH Amide 2.1x100mm；Mobile phase：Acetonitrile-water gradient is washed
De-, 0~15min keeps 87% acetonitrile, 15~20min to gradually decrease down 15% acetonitrile by 87% acetonitrile, and 20~30min is protected
Hold 15% acetonitrile；Percentage in mobile phase is percent by volume；
Mass Spectrometry Conditions are：The sources ESI, Salbutamol Selected Ion Monitoring（SIM）Pattern, fragmentation voltage（Fragmentor）：130v, sheath air-flow
Speed（Sheath gas flow）：12L/min, capillary voltage（Caplliary）：4000v, extraction accurate mass number [M-H]-
827.4, carry out linear quantitative with the common logarithm of concentration and peak area.
In specific chromatographic condition, flow velocity is 0.2 ~ 0.8mL/min；Column temperature is 37 ~ 42 DEG C；Sample size is 0.1 ~ 1 μ L；It is excellent
The chromatographic condition of choosing is：Flow velocity is 0.4mL/min；Column temperature is 40 DEG C；Sample size is 0.5 μ L.
In step（1）In, maltopentaose linear solvent concentration range is：1.775-28.4μg/mL.
In step（1）In, maltopentaose repeatability solution concentration is：7.1μg/mL.
In step（1）In, maltopentaose detection limit solution concentration is：0.355μg/mL.
In step（2）In, chromatographic column is Waters Xbridge BEH Amide 2.1x100mm, Part in chromatographic condition
No.:186006091。
In step（2）In, fragmentation voltage is respectively set in Mass Spectrometry Conditions：40,50,60,70,80,90,100,110,
120,130,140, extraction accurate mass number [M-H]-827.4 Abundances are compared, when fragmentation voltage is 130v, Abundances
Highest.
In step（2）In, using the common logarithm of maltopentaose standard working solution concentration as abscissa, with [the M- of extraction
H]-827.4 peak area common logarithm is ordinate, draws standard curve and is quantified.
The method of detection maltopentaose provided by the invention has following advantages：（1）It is qualitative accurate（2）High sensitivity（3）
Precision is high（4）The right height of Linear Quasi（5）It is easy to operate, it is suitable for the analysis detection of maltopentaose.
Since maltopentaose has two kinds of isomerisms of α, β, it is easy to anomaly peak is presented in chromatography bimodal or drag
End peak, to qualitative and quantitatively bring interference.Under the liquid-mass chromatography testing conditions of the present invention, unimodal, peak shape is presented in maltopentaose
Good, data processing is more accurate.
The difficult point of maltopentaose analysis detection is its, and ultraviolet colour developing is weak, boiling point is high, difficult volatilization, is not easy to gasify, ultraviolet inspection
It surveys and the means such as vapor detection is difficult to detect, the accuracy of differential pulse polarograpll is low.The present invention uses liquid-mass chromatography detection side
Method, high sensitivity, favorable reproducibility.
Containing there are five repetitive unit in maltopentaose molecular structure, C-O keys when Mass Spectrometer Method between repetitive unit are easily broken
It splits and generates a large amount of fragment ion peaks, cause molecular ion peak abundance low, error and inconvenience are brought to actual analysis.The present invention passes through
Using mass spectrum Salbutamol Selected Ion Monitoring (SIM) pattern, avoid generating a large amount of fragment ions, the abundance of maltopentaose molecular ion peak
Height, with [M-H]-Form detects.
The method of the present invention is quantified using the logarithm of concentration and the power function equation of peak area logistic fit, linear to close
It fastens（R2=0.9997）, make the accuracy higher of quantitative detection, solve routine when detecting saccharide compound with mass detector
The fitting of concentration and peak area linear equation linear poor problem.
Description of the drawings
Fig. 1 is the linear equation standard curve of the method for the present invention embodiment 1.
Fig. 2 is the power function equation standard curve of the method for the present invention embodiment 1.
Fig. 3 is the repetitive nature spectrogram of the method for the present invention embodiment 2.
Fig. 4 is the detection limit mass spectrogram of the method for the present invention embodiment 3.
Fig. 5 is the different fragmentation voltage curves of the method for the present invention embodiment 4.
Fig. 6 is the corresponding mass spectrograms of fragmentation voltage 130v of the method for the present invention embodiment 4.
Fig. 7 is the testing result figure that the mobile phase of embodiment 5 is screened.
Fig. 8 is the testing result figure of the maltopentaose and monosaccharide, disaccharides separating effect of embodiment 6.
Fig. 9 is the testing result figure of the maltopentaose and ketose separating effect of embodiment 6.
Figure 10 A-10B are maltopentaose testing result figure under the different column temperatures of embodiment 7.
Figure 10 C are maltopentaose abundance of ions value curve graph under the different column temperatures of embodiment 7.
Specific implementation mode
Clear, complete description will be carried out to technical scheme of the present invention below, it is clear that described embodiment is this hair
Bright a part of the embodiment, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art exist
The every other embodiment obtained under the premise of not making creative work, shall fall within the protection scope of the present invention.
Embodiment 1：
Chromatographic condition：Agilent1290；Chromatographic column：Waters Xbridge BEH Amide (2.1x100mm), Part
No.：186006091；Mobile phase：Acetonitrile-water gradient, 0~15min, 87% acetonitriles, 15~20min, 87% acetonitriles-
15% acetonitrile, 20~30min, 15% acetonitriles；Flow velocity：0.4mL/min；Column temperature：40℃；Sample size：0.5μL.
Mass Spectrometry Conditions：Agilent6460；The sources ESI, SIM patterns, fragmentation voltage：130v, sheath gas：12L/min, hair
Tubule voltage：4000v, extraction accurate mass number 827.4（[M-H]-）, carried out with the common logarithm of concentration and peak area linear
It is quantitative.
The preparation of test solution：Maltopentaose 2.84mg is taken, it is accurately weighed, it sets in 10mL volumetric flasks, water dissolution is added
And constant volume, it shakes up, precision pipettes 1mL, sets in 10mL volumetric flasks, and water dissolution and constant volume is added, shakes up, and is prepared into maltopentaose mother
Liquid, it is a concentration of：28.4μg/mL；Dilute 2 times step by step successively, configuration maltopentaose linear solvent concentration is respectively：28.4μg/mL,
14.2μg/mL、7.1μg/mL、3.55μg/mL、1.775μg/mL。
Measuring method：Precision draws 0.5 μ L of test solution, and injection ultra performance liquid chromatography-mass spectrometer measures, with mark
Quasi- working solution concentration is abscissa, with [M-H]-Peak area is ordinate, is drawn respectively with linear equation and power function equation
Standard curve.
As a result：Linear equation be y=993.72x+775.99, related coefficient 0.9991, power function equation be y=
0.9161x+3.1261, related coefficient 0.9997；It follows that the corresponding calibration curve coefficient correlation of power function equation is more
Height is specifically shown in the following table 1 and attached drawing 1, attached drawing 2.
1 maltopentaose standard curve of table
Conventional standard curve is the linear equation of concentration and peak area fitting；When detecting saccharide compound with mass detector,
It was found that sample introduction quality and Mass Spectrometer Method response do not present good linear, therefore quantified using conventional standard curve
When, it may occur that sample peak area poor repeatability, it is difficult to reach accurate quantitative analysis.And use the corresponding standard curve phase of power function equation
Relationship number higher.
Embodiment 2：
Chromatographic condition：Agilent1290；Chromatographic column：Waters Xbridge BEH Amide (2.1x100mm), Part
No.：186006091；Mobile phase：Acetonitrile-water gradient, 0~15min, 87% acetonitriles, 15~20min, 87% acetonitriles-
15% acetonitrile, 20~30min, 15% acetonitriles；Flow velocity：0.4mL/min；Column temperature：40℃；Sample size：0.5μL.
Mass Spectrometry Conditions：Agilent6460；The sources ESI, SIM patterns, fragmentation voltage：130v, sheath gas：12 L/min, hair
Tubule voltage：4000v, extraction accurate mass number 827.4（[M-H]-）, carried out with the common logarithm of concentration and peak area linear
It is quantitative.
The preparation of test solution：Maltopentaose 2.84mg is taken, it is accurately weighed, it sets in 10ml volumetric flasks, water dissolution is added
And constant volume, it shakes up, precision pipettes 1ml, sets in 10ml volumetric flasks, and water dissolution and constant volume is added, shakes up, and is prepared into maltopentaose mother
Liquid, it is a concentration of：28.4μg/mL；It dilutes step by step successively, configuration maltopentaose repeatability solution concentration is：7.1μg/mL.
Measuring method：Precision draws 0.5 μ L of test solution, injects ultra performance liquid chromatography-mass spectrometer, continuous sample introduction
7 needles calculate [M-H]-The RSD values of the common logarithm of peak area.
As a result：Relative standard deviation is 1.4% < 2%, illustrates reproducible, meets the requirements, is specifically shown in the following table 2 and attached drawing 3.
2 maltopentaose Precision Experiment of table
Embodiment 3：
Chromatographic condition：Agilent1290；Chromatographic column：Waters Xbridge BEH Amide (2.1x100mm), Part
No.：186006091；Mobile phase：Acetonitrile-water gradient, 0~15min, 87% acetonitriles, 15~20min, 87% acetonitriles-
15% acetonitrile, 20~30min, 15% acetonitriles；Flow velocity：0.4mL/min；Column temperature：40℃；Sample size：0.5μL.
Mass Spectrometry Conditions：Agilent6460；The sources ESI, SIM patterns, fragmentation voltage：130v, sheath gas：12L/min, hair
Tubule voltage：4000v, extraction accurate mass number 827.4（[M-H]-）, carried out with the common logarithm of concentration and peak area linear
It is quantitative.
The preparation of test solution：Maltopentaose 2.84mg is taken, it is accurately weighed, it sets in 10ml volumetric flasks, water dissolution is added
And constant volume, it shakes up, precision pipettes 1ml, sets in 10ml volumetric flasks, and water dissolution and constant volume is added, shakes up, and is prepared into maltopentaose mother
Liquid, it is a concentration of：28.4μg/mL；It dilutes step by step successively, configuration maltopentaose detection limit solution concentration is：0.355μg/mL.
Measuring method：Precision draws 0.5 μ L of test solution, injects ultra performance liquid chromatography-mass spectrometer, calculates noise
Than.
As a result：When maltopentaose sample introduction quality is 0.125ng（A concentration of 0.25 μ g/mL, sample size are 0.5 μ L）When, letter
It makes an uproar than being 3.It is specifically shown in attached drawing 4.
Embodiment 4：
Chromatographic condition：Agilent1290；Chromatographic column：Waters Xbridge BEH Amide (2.1x100mm), Part
No.：186006091；Mobile phase：Acetonitrile-water gradient, 0~15min, 87% acetonitriles, 15~20min, 87% acetonitriles-
15% acetonitrile, 20~30min, 15% acetonitriles；Flow velocity：0.4mL/min；Column temperature：40℃；Sample size：0.5μL.
Mass Spectrometry Conditions：Agilent6460；The sources ESI, SIM patterns, fragmentation voltage are respectively set to：40v, 50v, 60v,
70v, 80v, 90v, 100v, 110v, 120v, 130v, 140v, sheath gas：12L/min, capillary voltage：4000v.
The preparation of test solution：Maltopentaose 2.84mg is taken, it is accurately weighed, it sets in 10ml volumetric flasks, water dissolution is added
And constant volume, it shakes up, precision pipettes 1ml, sets in 10ml volumetric flasks, and water dissolution and constant volume is added, shakes up, and is prepared into maltopentaose mother
Liquid, it is a concentration of：28.4μg/mL；It dilutes step by step successively, configuration maltopentaose is a concentration of：7.1μg/mL.
Measuring method：Precision draws 0.5 μ L of test solution, injects ultra performance liquid chromatography-mass spectrometer, and extraction is accurate
Mass number 827.4（[M-H]-）Abundances are compared, and determine optimal fragmentation voltage value.
As a result：When fragmentation voltage is set as 130v,（[M-H]-）Abundances highest.It is specifically shown in the following table 3 and attached drawing 5, it is attached
Fig. 6.The corresponding mass spectrograms of fragmentation voltage 130v are illustrated in figure 6, molecular ion peak [M-H] is shown in collection of illustrative plates- 827.4 is very aobvious
It writes, abundance is high, is convenient for atlas analysis.
The different fragmentation voltages of table 3（[M-H]-）Abundances
| Fragmentation voltage（v） | （[M-H]-）Abundances |
| 40 | 518.50 |
| 50 | 525.19 |
| 60 | 550.43 |
| 70 | 553.88 |
| 80 | 573.25 |
| 90 | 604.26 |
| 100 | 617.01 |
| 110 | 633.32 |
| 120 | 615.58 |
| 130 | 637.59 |
| 140 | 621.71 |
Embodiment 5：
Chromatographic condition：Agilent1290；Chromatographic column：Waters Xbridge BEH Amide (2.1x100mm), Part
No.：186006091；Flow velocity：0.4mL/min；Column temperature：40℃；Sample size：0.5μL.
Mass Spectrometry Conditions：Agilent6460；The sources ESI, SIM patterns, fragmentation voltage：130v, sheath gas：12L/min, hair
Tubule voltage：4000v, extraction accurate mass number 827.4（[M-H]-）.
Mobile phase 1：Acetonitrile-water=75:25 isocratic operation 30min.
Mobile phase 2：Acetonitrile-water gradient, 0~15min, 87% acetonitriles, -15% second of 15~20min, 87% acetonitriles
Nitrile, 20~30min, 15% acetonitriles.
The preparation of test solution：Maltopentaose 10.84mg is taken, it is accurately weighed, it sets in 10ml volumetric flasks, water dissolution is added
And constant volume, it shakes up, precision pipettes 1ml, sets in 10ml volumetric flasks, and water dissolution and constant volume is added, shakes up, and is prepared into maltopentaose mother
Liquid, it is a concentration of：100.84μg/mL；Dilution, configuration maltopentaose are a concentration of：10.84μg/mL.
Measuring method：Precision draws 0.5 μ L of test solution, injects ultra performance liquid chromatography-mass spectrometer, more different
The operation result of mobile phase.
As a result：Acetonitrile-water=75:25 isocratic operation 30min, the non-appearance of maltopentaose；Acetonitrile-water gradient, malt five
Sugar occurs unimodal.It is specifically shown in attached drawing 7.
Embodiment 6：
Chromatographic condition：Agilent1290；Chromatographic column：Waters Xbridge BEH Amide (2.1x100mm), Part
No.：186006091；Mobile phase：Acetonitrile-water gradient, 0~15min, 87% acetonitriles, 15~20min, 87% acetonitriles-
15% acetonitrile, 20~30min, 15% acetonitriles；Flow velocity：0.4mL/min；Column temperature：40℃；Sample size：0.5μL.
Mass Spectrometry Conditions：Agilent6460；The sources ESI, SIM patterns, fragmentation voltage：130v, sheath gas：12L/min, hair
Tubule voltage：4000v, extraction accurate mass number 827.4（[M-H]-）, carried out with the common logarithm of concentration and peak area linear
It is quantitative.
Measuring method：Take maltopentaose and monosaccharide（Such as fructose）, disaccharides（Such as sucrose）Mixing sample feeding, detect malt five
Sugar and monosaccharide, the separating effect of disaccharides.Maltopentaose and ketose mixing sample introduction are taken, the separation of maltopentaose and trisaccharide is detected
Effect.
As a result：Maltopentaose and monosaccharide, disaccharides mixing sample introduction, testing result are as shown in Figure 8, wherein the guarantor of maltopentaose
Stay time t=18.3min, retention time t=10.1min of sucrose, retention time t=3.0min of fructose.Maltopentaose and sugarcane fruit
Trisaccharide mixing sample introduction, testing result as shown in figure 9, maltopentaose retention time t=18.4mi, the retention time t of ketose
The separating degree of=17.7min, maltopentaose and ketose is up to 1.2.It follows that method using the present invention, it can be by malt
Pentasaccharides is detached with other monosaccharide, disaccharides, trisaccharide realization, good separating effect.
Embodiment 7：
Chromatographic condition：Agilent1290；Chromatographic column：Waters Xbridge BEH Amide (2.1x100mm), Part
No.：186006091；Mobile phase：Acetonitrile-water gradient, 0~15min, 87% acetonitriles, 15~20min, 87% acetonitriles-
15% acetonitrile, 20~30min, 15% acetonitriles；Flow velocity：0.4mL/min；Column temperature：25℃,30℃,35℃,37℃,40℃,42
℃,45℃,50℃,60℃；Sample size：0.5μL.
Mass Spectrometry Conditions：Agilent6460；The sources ESI, SIM patterns, fragmentation voltage：130v, sheath gas：12L/min, hair
Tubule voltage：4000v, extraction accurate mass number 827.4（[M-H]-）, carried out with the common logarithm of concentration and peak area linear
It is quantitative.
The preparation of test solution：Maltopentaose 10.84mg is taken, it is accurately weighed, it sets in 10ml volumetric flasks, water dissolution is added
And constant volume, it shakes up, precision pipettes 1ml, sets in 10ml volumetric flasks, and water dissolution and constant volume is added, shakes up, and is prepared into maltopentaose mother
Liquid, it is a concentration of：100.84μg/mL；Dilution, configuration maltopentaose are a concentration of：10.84μg/mL.
Measuring method：Precision draws 0.5 μ L of test solution, injects ultra performance liquid chromatography-mass spectrometer, and extraction is accurate
Mass number 827.4（[M-H]-）Abundances are compared, and determine most suitable column temperature.
As a result：Testing result such as Figure 10 A, 10B, shown in 10C, wherein Figure 10 A show maltopentaose in 25 DEG C of -40 DEG C of columns
Ion flow graph under temperature, from this figure, it can be seen that maltopentaose is when temperature is relatively low（25~35℃）Significantly hangover is presented
Peak；Figure 10 B show ion flow graph of the maltopentaose under different 42 DEG C of -60 DEG C of column temperatures, from this figure, it can be seen that when temperature increases
When, it gradually presents unimodal；But when temperature is more than 45 DEG C, abundance of ions value is substantially reduced, and as illustrated in figure 10 c, abscissa is temperature
Degree, ordinate are abundance of ions value；And column temperature is excessively high, influences using life of chromatographic column.Therefore select column temperature Celsius for 37 ~ 42
Degree, more preferable 40 DEG C of analysis temperatures as maltopentaose.
In conclusion the various embodiments described above are only presently preferred embodiments of the present invention, not limiting the present invention's
Protection domain, all within the spirits and principles of the present invention, any modification, equivalent substitution, improvement and etc. done should be all included in
In protection scope of the present invention.
Claims (10)
1. a kind of method detecting maltopentaose using ultra performance liquid chromatography-mass spectrometric hyphenated technique, which is characterized in that including with
Lower step：
（1）It takes maltopentaose appropriate, is dissolved in water, be configured to certain density test solution；
（2）Sample introduction is detected by following chromatographic condition and Mass Spectrometry Conditions；
Chromatographic condition is：Chromatographic column：Waters Xbridge BEH Amide 2.1x100mm；Mobile phase：Acetonitrile-water gradient is washed
De-, 0~15min keeps 87% acetonitrile, 15~20min to gradually decrease down 15% acetonitrile by 87% acetonitrile, and 20~30min is kept
15% acetonitrile；Column temperature is 37 ~ 42 DEG C；
Mass Spectrometry Conditions are：The sources ESI, SIM patterns, fragmentation voltage：130v, sheath gas：12L/min, capillary voltage：
4000v, extraction accurate mass number [M-H]-827.4, carry out linear quantitative with the common logarithm of concentration and peak area.
2. according to the method described in claim 1, it is characterized in that, in the chromatographic condition, flow velocity is 0.2 ~ 0.8mL/min；
Sample size is 0.1 ~ 1 μ L.
3. according to the method described in claim 2, it is characterized in that, in the chromatographic condition, flow velocity 0.4mL/min；Sample introduction
Amount is 0.5 μ L.
4. according to the method described in claim 1, it is characterized in that, in the chromatographic condition, column temperature is 40 DEG C.
5. according to the method described in claim 1, it is characterized in that, the step（1）In, maltopentaose linear solvent concentration model
Enclose for：1.775-28.4μg/mL.
6. according to the method described in claim 5, it is characterized in that, the step（1）In, configuration maltopentaose linear solvent is dense
Degree is respectively：28.4μg/mL,14.2μg/mL,7.1μg/mL,3.55μg/mL,1.775μg/mL.
7. according to the method described in claim 1, it is characterized in that, the step（1）In, maltopentaose repeatability solution concentration
For：7.1μg/mL.
8. according to the method described in claim 1, it is characterized in that, the step（1）In, maltopentaose detection limit solution concentration
For：0.355μg/mL.
9. according to the method described in claim 1, it is characterized in that, the step（2）In, it is worked with maltopentaose standard molten
The common logarithm of liquid concentration is abscissa, with [M-H] of extraction-827.4 peak area common logarithm is ordinate, with power letter
Number equation is drawn standard curve and is quantified.
10. according to the method described in claim 9, it is characterized in that, the coefficient R of the power function equation2=0.9997。
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Cited By (3)
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| CN109374779A (en) * | 2018-12-17 | 2019-02-22 | 杭州奕安济世生物药业有限公司 | A kind of rapid detection method of cane sugar content |
| CN112903839A (en) * | 2021-01-15 | 2021-06-04 | 中国检验检疫科学研究院 | Method for qualitatively detecting alpha and beta configurational isomers of monosaccharide and method for detecting proportion of monosaccharide isomers |
| CN112903842A (en) * | 2021-01-18 | 2021-06-04 | 中国检验检疫科学研究院 | Method for detecting monosaccharide |
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| CN112903842A (en) * | 2021-01-18 | 2021-06-04 | 中国检验检疫科学研究院 | Method for detecting monosaccharide |
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