CN108732231A - The chitosan-modified glass-carbon electrode of soluble starch-and its application - Google Patents
The chitosan-modified glass-carbon electrode of soluble starch-and its application Download PDFInfo
- Publication number
- CN108732231A CN108732231A CN201810309753.9A CN201810309753A CN108732231A CN 108732231 A CN108732231 A CN 108732231A CN 201810309753 A CN201810309753 A CN 201810309753A CN 108732231 A CN108732231 A CN 108732231A
- Authority
- CN
- China
- Prior art keywords
- chitosan
- soluble starch
- carbon electrode
- solution
- modified glass
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/48—Systems using polarography, i.e. measuring changes in current under a slowly-varying voltage
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/308—Electrodes, e.g. test electrodes; Half-cells at least partially made of carbon
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Carbon And Carbon Compounds (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
The invention discloses a kind of chitosan-modified glass-carbon electrode of soluble starch-and its applications, ultrasound prepares composite material after the chitosan-modified glass-carbon electrode of soluble starch-is mixed using soluble starch solution with chitosan solution, composite material drop coating is made in glassy carbon electrode surface after drying.The chitosan-modified glass-carbon electrode of soluble starch-effectively utilizes the film forming ability of soluble starch and chitosan, realizes the identification to amino acid enantiomer, the especially identification to amino acid enantiomers such as tyrosine, tryptophan, histidine, phenylalanine, proline.
Description
Technical field
The present invention relates to electrochemical technology fields, it particularly relates to a kind of chitosan-modified glass carbon electricity of soluble starch-
The application of the chitosan-modified glass-carbon electrode detection amino acid enantiomer of soluble starch-is especially used in pole and its application.
Background technology
Chirality is a kind of chemical characteristic, and in biology, medical science and biotechnology field are played the part of on a molecular scale
Important role.In addition to glycine, almost all of amino acid all has Chiral properties.Therefore, these amino acid can be made
For the important biomolecule marker of various metabolic diseases.Since two kinds of chiral isomers have almost the same chemically and physically property
Matter, therefore be difficult to distinguish L/D type amino acid enantiomers.In order to screen, diagnosing and treating metabolic disease is developed a kind of effective
, sensitively, selective quantitative analysis method is an important task.
Starch is the high polymer long chain compound formed by glucosides key connection by many hydroxyl glucose units, chemistry
Structural formula is (C5H10O5)n.Soluble starch (Soluble starch, SS) is a kind of white or off-white powder, odorless nothing
Taste does not dissolve in cold water, can then become clear solution in the hot water, not lump after cooling, with certain viscosity, and with compared with
Good film forming ability, the stability of electrode can be improved by being applied to the modification of glassy carbon electrode surface.
The polysaccharide chitosan (Chitosan, CS) of biocompatibility by random distribution β-(1-4)-connections d-glucosamine
With N- acetyl-d-glucosamine composition.Due to there is a large amount of chiral site, CS and its derivative also show that high chiral selection
Property, and it is widely used as chiral resolving agent.
Current study show that by Chiral carbon nanotube, conducting polymer, carbon quantum dot, potato starch, beta-cyclodextrin,
Human serum albumins and chitosan apply to the modification of glassy carbon electrode surface, can effectively improve modified electrode to amino acid enantiomer
Recognition capability.In order to further improve the stability and recognition capability of electrode detection amino acid enantiomer, continue to probe into modification
Material to the modification of glass-carbon electrode be still extremely have it is urgent.
Invention content
For above-mentioned technical problem in the related technology, the present invention proposes a kind of chitosan-modified glass carbon of soluble starch-
Electrode and its application can be effectively improved modified electrode by modifying glass-carbon electrode soluble starch and chitosan
The unstable problem of electrochemistry can significantly improve chiral recognition of the modified electrode to amino acid enantiomer.
To realize the above-mentioned technical purpose, the technical proposal of the invention is realized in this way：
On the one hand, the present invention provides a kind of preparation method of the chitosan-modified glass-carbon electrode of soluble starch-, including as follows
Step：
1) soluble starch solution and chitosan solution are prepared；
2) ultrasound prepares composite material after mixing soluble starch solution with chitosan solution；
3) it is made soluble by composite material drop coating obtained by step 2) in glassy carbon electrode surface, after drying to form sediment
The chitosan-modified electrode of powder-.
Further, the process for preparation of the soluble starch solution is as follows：Soluble starch and ultra-pure water are pressed into quality
Than reacting 5~60min at 100~130 DEG C after 1: 1~8 mixing, it is cooled to room temperature obtained.Preferably, the soluble starch
Mass ratio with ultra-pure water is 1: 1,1: 2,1: 3,1: 4,1: 5,1: 6 etc..
Further, the process for preparation of chitosan solution is as follows：Will chitosan be added dilute acid soln in be made a concentration of 1~
5mg mL-1Chitosan solution.It is further preferred that a concentration of 0.1~5%, the v/v of the dilute acid soln；The diluted acid is molten
Liquid is formulated with one or more of hydrochloric acid, sulfuric acid, nitric acid, acetum with ultra-pure water.Preferably, the chitosan
Solution concentration is 1mg mL-1、2mg mL-1、3mg mL-1、4mg mL-1、5mg mL-1.Preferably, the dilute acid soln is dilute salt
Acid, dilute sulfuric acid, dust technology, spirit of vinegar etc., a concentration of 0.1% counted carefully, 0.5%, 0.2%, 1%, 1.5%, 2%,
3%, 4%, 5% etc..
Further, in the step 2), the volume ratio of soluble starch solution and chitosan solution is 1~10: 1.It can
The volume ratio of soluble starch solution and chitosan solution is 1: 1,1.5: 1,2: 1,2.5: 1,3: 1,3.5：1,4:1,6:1,5:1,8
: 1,9: 1,10: 1 etc..
Further, in the step 2), the ultrasonic time is 10~60min.Preferably, ultrasonic time 10min,
15min, 20min, 30min, 40min, 60min etc..
Further, the dosage of composite material obtained by step 2) is 2~10 μ L in the step 3).The dosage of composite material
For 2 μ L, 3 μ L, 5 μ L, 6 μ L, 8 μ L, 10 μ L etc..
Further, in the step 3), dry using one or more of air, infrared lamp, nitrogen stream mode into
Row dries.
On the other hand, the present invention provides a kind of chitosan-modified glass-carbon electrode of soluble starch-, including with of the present invention
Method be prepared.
On the other hand, the present invention provides a kind of method of detection amino acid enantiomer, with method system of the present invention
The chitosan-modified glass-carbon electrode of standby soluble starch-carries out in electrolyte solution.The amino acid is tyrosine, color ammonia
Acid, histidine, phenylalanine and proline etc..
Further, the electrolyte solution is one or more of inorganic salts, inorganic acid buffer solution solution.
Beneficial effects of the present invention：A kind of chitosan-modified glass-carbon electrode of soluble starch-of present invention offer and its application,
The chitosan-modified glass-carbon electrode of soluble starch-utilizes the film forming ability of soluble starch and chitosan, realizes to amino
The identification of sour enantiomer carries out effectively identification L/D types especially to tyrosine, tryptophan, histidine, phenylalanine, proline etc.
Enantiomer.The composite material has homogeneity, is coated in glassy carbon electrode surface and forms a film, the synergistic effect film forming based on composite material
Afterwards so that prepare glass-carbon electrode Electrochemical Detection amino acid L/D type enantiomers when, can get stable current-responsive, show
To the good qualitative and quantitative analysis application prospect of amino acid enantiomer.The composite wood of used soluble starch and chitosan
Expect that environmentally protective, stability is good, be expected to become widely applied electrode modified material.
Description of the drawings
It in order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, below will be to institute in embodiment
Attached drawing to be used is needed to be briefly described, it should be apparent that, the accompanying drawings in the following description is only some implementations of the present invention
Example, for those of ordinary skill in the art, without creative efforts, can also obtain according to these attached drawings
Obtain other attached drawings.
Fig. 1 is the chitosan-modified glass-carbon electrode of soluble starch-(SS-CS/GCE, Fig. 1 C), bare electrode (bare GCE, figure
1A), the square wave volt of soluble starch modified electrode (SS/GCE, Figure 1B) and overall contrast figure (Fig. 1 D) detection tyrosine enantiomer
Pacify curve comparison figure；
Fig. 2 is the square wave volt-ampere curve figure that the chitosan-modified glass-carbon electrode of soluble starch-detects tyrosine enantiomer；
Fig. 3 is the linear diagram that the chitosan-modified glass-carbon electrode of soluble starch-detects tyrosine enantiomer；
Fig. 4 is the potential difference comparison diagram that the chitosan-modified glass-carbon electrode of soluble starch-detects tyrosine enantiomer；
Fig. 5 is the square wave volt that the chitosan-modified glass-carbon electrode of soluble starch-(SS-CS/GCE) detects Tryptophan enantiomer
Pacify curve comparison figure；
Fig. 6 is the square wave volt that the chitosan-modified glass-carbon electrode of soluble starch-(SS-CS/GCE) detects Histidine enantiomer
Pacify curve comparison figure；
Fig. 7 is the square wave that the chitosan-modified glass-carbon electrode of soluble starch-(SS-CS/GCE) detects phenylalanine enantiomer
Volt-ampere curve comparison diagram；
Fig. 8 is the square wave volt that the chitosan-modified glass-carbon electrode of soluble starch-(SS-CS/GCE) detects proline enantiomers
Pacify curve comparison figure；
C in figureTyrTyrosine concentration；Potential current potentials；Current electric currents.
Specific implementation mode
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation describes, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, the every other embodiment that those of ordinary skill in the art are obtained belong to what the present invention protected
Range.
Unless otherwise defined, all technical terms used hereinafter and the normally understood meaning of those skilled in the art
It is identical.Technical term used herein is intended merely to the purpose of description specific embodiment, is not intended to the limitation present invention
Protection domain.
Except there is a special instruction, the various reagents used in the present invention, raw material be can be commercially commodity or
Person can pass through product made from well known method.
Embodiment 1
5mg soluble starches, 20mL ultra-pure waters are mixed, react 20min at 120 DEG C, natural cooling solution to room temperature waits for
With.
The 100mg chitosans aqueous solution of glacial acetic acid (1%, v/v) is configured to 1mg mL-1, for use.
Ultrasound 30min after taking soluble starch solution and chitosan solution to be mixed by 1: 1 (v/v).
8 μ L soluble starches-Chitosan Composites drop coating is pipetted in glassy carbon electrode surface, is dried in air.
Embodiment 2
20mg soluble starches, 40mL ultra-pure waters are mixed, react 60min at 130 DEG C, natural cooling solution to room temperature,
For use.
Dilute hydrochloric acid solution (5%, v/v) is added in 50mg chitosans and is configured to 5mg mL-1, for use.
Ultrasound 60min after taking soluble starch solution and chitosan solution to be mixed by 10: 1 (v/v).
10 μ L soluble starches-Chitosan Composites drop coating is pipetted in glassy carbon electrode surface, is dried under infrared lamp.
Embodiment 3
10mg soluble starches, 5mL ultra-pure waters are mixed, react 10min, natural cooling solution to room temperature at 100 DEG C.
The aqueous solution that 200mg chitosans are added to dilute sulfuric acid acid solution (0.1%, v/v) is configured to 3mg mL-1, for use.
Take soluble starch solution and chitosan solution by ultrasound 10min after 5: 1 (v/v) mixing.
2 μ L soluble starches-Chitosan Composites drop coating is pipetted in glassy carbon electrode surface, is dried in nitrogen stream.
Embodiment 4
15mg soluble starches, 20mL ultra-pure waters are mixed, react 5min, natural cooling solution to room temperature at 130 DEG C.
The aqueous solution that 100mg chitosans are added to dust technology acid solution (2%, v/v) is configured to 2.5mg mL-1, for use.
Ultrasound 60min after taking soluble starch solution and chitosan solution to be mixed by 8: 1 (v/v).
5 μ L soluble starches-Chitosan Composites drop coating is pipetted in glassy carbon electrode surface, is dried in air.
Embodiment 5 detects
Using the glass-carbon electrode prepared by embodiment 1 as working electrode, slide be to electrode, phosphate buffer solution (0.1M,
PH 7.0) in detection amino acid enantiomer (tyrosine, tryptophan, histidine, phenylalanine and proline is selected to be examined respectively
It surveys).Testing result is as follows：
Tyrosine (Tyr) enantiomer is analyzed using square wave voltammetry (SWV).It is can be found that from Figure 1A and Figure 1B naked
Electrode modified electrode (bare GCE) and soluble starch modified electrode (SS/GCE) are to the potential difference of tyrosine enantiomer
0mV, and the ratio of current-responsive is almost 1, illustrates that bare electrode modified electrode and soluble starch modified electrode cannot identify junket
Propylhomoserin enantiomer；The chitosan-modified electrode of soluble starch-(SS-CS/GCE) is can be seen that tyrosine enantiomer from Fig. 1 C
There is recognition reaction, since chitosan has the synergistic effect of a large amount of chiral site and soluble starch and chitosan so that system
The chitosan-modified glass-carbon electrode of standby soluble starch-shows higher chiral selectivity to tyrosine.
From figures 2 and 3, it will be seen that in the concentration range of 0.01-1mM, tyrosine (Tyr) mapping bulk concentration and peak value
Good linear relationship, L-Tyr and D-Tyr on the chitosan-modified electrode of soluble starch-(SS-CS/GCE) is presented in current value
It is respectively provided with the good coefficient of determination (R2 L-Tyr=0.98333 and R2 D-Trp=0.99631).
The chitosan-modified electrode of soluble starch-(SS-CS/GCE) detects electricity to L-Tyr and D-Tyr as can be seen from Figure 4
The stability of potential difference.
From Fig. 5~6 as can be seen that the chitosan-modified electrode of soluble starch-(SS-CS/GCE) tryptophan, histidine,
Phenylalanine and proline enantiomers have recognition reaction, due to chitosan exist a large amount of chiral site and soluble starch and
The synergistic effect of chitosan so that the chitosan-modified glass-carbon electrode tryptophan of soluble starch-, histidine, the phenylpropyl alcohol of preparation
Propylhomoserin and proline etc. all show higher chiral selectivity.
The method of the chitosan-modified electrode detection amino acid enantiomer of soluble starch-prepared by the present invention is convenient, efficiently,
It is time saving, expense is relatively low, can simply and quickly qualitative and quantitative analysis amino acid enantiomer.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
With within principle, any modification, equivalent replacement, improvement and so on should all be included in the protection scope of the present invention god.
Claims (10)
1. a kind of preparation method of the chitosan-modified glass-carbon electrode of soluble starch-, which is characterized in that include the following steps：
Prepare soluble starch solution and chitosan solution；
Ultrasound prepares composite material after soluble starch solution is mixed with chitosan solution；
By step 2）The chitosan-modified electricity of soluble starch-is made in glassy carbon electrode surface in gained composite material drop coating after drying
Pole.
2. the preparation method of the chitosan-modified glass-carbon electrode of soluble starch-according to claim 1, which is characterized in that
The process for preparation of the soluble starch solution is as follows：By soluble starch and ultra-pure water in mass ratio 1:100 after 1 ~ 8 mixing
5 ~ 60min is reacted at ~ 130 DEG C, is cooled to room temperature obtained.
3. the preparation method of the chitosan-modified glass-carbon electrode of soluble starch-according to claim 1, which is characterized in that
The process for preparation of the chitosan solution is as follows：Chitosan is added in dilute acid soln, a concentration of 1 ~ 5 mg mL are made-1Shell it is poly-
Sugar juice.
4. the preparation method of the chitosan-modified glass-carbon electrode of soluble starch-according to claim 3, which is characterized in that
A concentration of the 0.1 ~ 5% of the dilute acid soln,v/v；The dilute acid soln is with one in hydrochloric acid, sulfuric acid, nitric acid, acetum
Kind or several be formulated with ultra-pure water.
5. the preparation method of the chitosan-modified glass-carbon electrode of soluble starch-according to claim 1, which is characterized in that
The step 2）In, the volume ratio of soluble starch solution and chitosan solution is 1 ~ 10:1；
The step 2）In, the ultrasonic time is 10 ~ 60 min.
6. the preparation method of the chitosan-modified glass-carbon electrode of soluble starch-according to claim 1, which is characterized in that
The step 3）Middle step 2）The dosage of gained composite material is 2 ~ 10 μ L.
7. the preparation method of the chitosan-modified glass-carbon electrode of soluble starch-according to claim 1, which is characterized in that
The step 3）In, it dries and is dried using one or more of air, infrared lamp, nitrogen stream mode.
8. a kind of chitosan-modified glass-carbon electrode of soluble starch-, which is characterized in that the glass-carbon electrode is by claim 1 ~ 7
Any method is prepared.
9. a kind of method of detection amino acid enantiomer, which is characterized in that prepared with any method of claim 1 ~ 7
The chitosan-modified glass-carbon electrode of soluble starch-amino acid enantiomer is detected in electrolyte solution.
10. the method for detection amino acid enantiomer according to claim 9, which is characterized in that the amino acid includes junket
Propylhomoserin, tryptophan, histidine, phenylalanine or proline；The electrolyte solution is inorganic salt buffer solution, inorganic acid buffering
One or more of solution solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810309753.9A CN108732231B (en) | 2018-04-09 | 2018-04-09 | Soluble starch-chitosan modified glassy carbon electrode and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810309753.9A CN108732231B (en) | 2018-04-09 | 2018-04-09 | Soluble starch-chitosan modified glassy carbon electrode and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108732231A true CN108732231A (en) | 2018-11-02 |
| CN108732231B CN108732231B (en) | 2020-01-10 |
Family
ID=63940645
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810309753.9A Expired - Fee Related CN108732231B (en) | 2018-04-09 | 2018-04-09 | Soluble starch-chitosan modified glassy carbon electrode and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108732231B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110806437A (en) * | 2019-11-15 | 2020-02-18 | 中南大学 | Black phosphorus nanosheet/maltose- β -cyclodextrin modified glassy carbon electrode and application thereof |
| CN116574414A (en) * | 2023-05-16 | 2023-08-11 | 内江师范学院 | Graphite powder/starch chiral selector, glassy carbon electrode, preparation method and application |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105021681A (en) * | 2015-07-08 | 2015-11-04 | 常州大学 | Chitosan-modified glassy carbon electrode-based selective recognition of tryptophan enantiomer containing zinc ion |
| CN105044178A (en) * | 2015-04-23 | 2015-11-11 | 常州大学 | Chiral recognition to tryptophan enantiomer containing zinc ion by chiral sensor based on chitosan/sodium alginate |
-
2018
- 2018-04-09 CN CN201810309753.9A patent/CN108732231B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105044178A (en) * | 2015-04-23 | 2015-11-11 | 常州大学 | Chiral recognition to tryptophan enantiomer containing zinc ion by chiral sensor based on chitosan/sodium alginate |
| CN105021681A (en) * | 2015-07-08 | 2015-11-04 | 常州大学 | Chitosan-modified glassy carbon electrode-based selective recognition of tryptophan enantiomer containing zinc ion |
Non-Patent Citations (2)
| Title |
|---|
| LIPING BAO ET AL.: "Construction of Electrochemical Chiral Interfaces with Integrated Polysaccharides via Amidation", 《ACS APPL. MATER. INTERFACES》 * |
| LIPING BAO ET AL.: "Potato starch as a highly enantioselective system for temperature-dependent electrochemical recognition of tryptophan isomers", 《ELECTROCHEMISTRY COMMUNICATIONS》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110806437A (en) * | 2019-11-15 | 2020-02-18 | 中南大学 | Black phosphorus nanosheet/maltose- β -cyclodextrin modified glassy carbon electrode and application thereof |
| CN110806437B (en) * | 2019-11-15 | 2020-08-04 | 中南大学 | Black phosphorus nanosheet/maltose- β -cyclodextrin modified glassy carbon electrode and application thereof |
| CN116574414A (en) * | 2023-05-16 | 2023-08-11 | 内江师范学院 | Graphite powder/starch chiral selector, glassy carbon electrode, preparation method and application |
| CN116574414B (en) * | 2023-05-16 | 2024-05-28 | 内江师范学院 | Graphite powder/starch chiral selector, glassy carbon electrode, preparation method and application |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108732231B (en) | 2020-01-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Joshi et al. | Recent advances in nanomaterial-based electrochemical detection of antibiotics: Challenges and future perspectives | |
| Sabaté del Río et al. | An antifouling coating that enables affinity-based electrochemical biosensing in complex biological fluids | |
| Lu et al. | Electrochemical determination of rutin based on molecularly imprinted poly (ionic liquid) with ionic liquid-graphene as a sensitive element | |
| Gan et al. | A highly sensitive electrochemiluminescence immunosensor for h-FABP determination based on self-enhanced luminophore coupled with ultrathin 2D nickel metal-organic framework nanosheets | |
| Lin et al. | A novel electrochemical sensor for the analysis of β-agonists: the poly (acid chrome blue K)/graphene oxide-nafion/glassy carbon electrode | |
| Khan et al. | Nanocrystalline bioactive TiO2–chitosan impedimetric immunosensor for ochratoxin-A | |
| Babaei et al. | Nanomolar simultaneous determination of levodopa and serotonin at a novel carbon ionic liquid electrode modified with Co (OH) 2 nanoparticles and multi-walled carbon nanotubes | |
| Wang et al. | Surface plasmon resonance sensor chips for the recognition of bovine serum albumin via electropolymerized molecularly imprinted polymers | |
| de Oliveira et al. | Immobilization procedures for the development of a biosensor for determination of hydroquinone using chitosan and gilo (Solanum gilo) | |
| Xu et al. | A novel molecularly imprinted sensor for selectively probing imipramine created on ITO electrodes modified by Au nanoparticles | |
| Fernandes-Junior et al. | Electrochemical sensor based on nanodiamonds and manioc starch for detection of tetracycline | |
| Nevado et al. | Spectrofluorimetric study of the β-cyclodextrin: vitamin K3 complex and determination of vitamin K3 | |
| Li et al. | Electrolytic conductivity behaviors and solution conformations of chitosan in different acid solutions | |
| CN106883849A (en) | Graphene quantum dot that a kind of nitrogenous sulphur mixes and preparation method thereof and the application on lysine luciferase assay reagent is prepared | |
| Yu et al. | A sensitive molecularly imprinted electrochemical aptasensor for highly specific determination of melamine | |
| Taei et al. | Simultaneous determination of cysteine, uric acid and tyrosine using Au-nanoparticles/poly (E)-4-(p-tolyldiazenyl) benzene-1, 2, 3-triol film modified glassy carbon electrode | |
| Li et al. | A gold nanoparticles-based molecularly imprinted electrochemical sensor for histamine specific-recognition and determination | |
| Song et al. | Gemini surfactant applied to the heparin assay at the nanogram level by resonance Rayleigh scattering method | |
| Khumsap et al. | Epitope-imprinted polydopamine electrochemical sensor for ovalbumin detection | |
| CN108017725B (en) | Preparation and application of multiwalled carbon nanotube-N-carboxymethyl chitosan chiral material | |
| Zhu et al. | Voltammetric behavior and determination of phenylephrine at a glassy carbon electrode modified with multi-wall carbon nanotubes | |
| Zhou et al. | Application of hydrogel prepared from ferrocene functionalized amino acid in the design of novel electrochemical immunosensing platform | |
| CN108732231A (en) | The chitosan-modified glass-carbon electrode of soluble starch-and its application | |
| Niu et al. | Chiral voltammetric sensor for tryptophan enantiomers by using a self‐assembled multiwalled carbon nanotubes/polyaniline/sodium alginate composite | |
| Cheng et al. | “Off-On” switching electrochemiluminescence biosensor for mercury (II) detection based on molecular recognition technology |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200110 Termination date: 20210409 |