CN108732110A - A kind of analysis method of banana stalk chemical analysis - Google Patents
A kind of analysis method of banana stalk chemical analysis Download PDFInfo
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- CN108732110A CN108732110A CN201810884810.6A CN201810884810A CN108732110A CN 108732110 A CN108732110 A CN 108732110A CN 201810884810 A CN201810884810 A CN 201810884810A CN 108732110 A CN108732110 A CN 108732110A
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Abstract
The invention discloses a kind of analysis methods of banana stalk chemical analysis, include the following steps：Material prepares, rubber banana stalk sample is put into pond, it is rinsed by tap water, chopping, it is dry in aeration cabinet, record moisture, sample is crushed with pulverizer, it is sieved with sieve, obtain Sample A, and Sample A is preserved at room temperature for use, buck, Sample A is ground into sieving, extraction obtains sample B, again through vacuum filter, and it is dry in aeration cabinet, soluble sugar measures, sample B is dried to weight, mixing, weigh dry weight sample, appropriate phosphate buffer is added fully to mill, it shifts in centrifuge tube, constant volume, mixing sample, centrifugal treating.The present invention improves the utilization rate of sample, is conducive to control experimental variable, improves the reliability of analysis result so that the analysis result of banana stalk chemical analysis is relatively reliable, is conducive to the utilization of banana stalk, rationally utilizes resource.
Description
Technical field
The present invention relates to the analysis technical field of banana stalk chemical analysis more particularly to a kind of banana stalk chemical analysis
Analysis method.
Background technology
Banana all one's life one inferior fruit of knot, is left a large amount of agricultural residues, these residues cause pest and disease damage propagation and environment
Pollution.It can be converted into ethyl alcohol using the lignocellulosic extracted in banana residue as bioenergy, or carry out resource
Change and utilizes（Such as feed, fertilizer）, reach improvement agricultural environment, improve the purpose of land output.Accurate analysis banana stalk is thin
Cell wall composition determines that its different purposes is all particularly important for precisely identifying banana variety, existing analysis method, Bu Nengzhun
The chemical composition of true analysis banana stalk, it has not been convenient to which banana stalk recycles, so now proposing a kind of banana stalk chemistry
The analysis method of composition.
Invention content
Technical problems based on background technology, the present invention propose a kind of analysis side of banana stalk chemical analysis
Method.
A kind of analysis method of banana stalk chemical analysis proposed by the present invention, includes the following steps：
S1：Material prepares, and banana stalk sample is put into pond, is rinsed by tap water, chopping, is done in aeration cabinet
It is dry, moisture is recorded, sample is crushed with pulverizer, sieved with sieve, obtains Sample A, and Sample A is preserved at room temperature and is waited for
With；
S2：Sample A is ground sieving by buck, and extraction obtains sample B, then through vacuum filter, and is done in aeration cabinet
It is dry；
S3：Soluble sugar measures, and sample B is dried to weight, mixing weighs dry weight sample, and appropriate phosphate buffer is added and fills
Divide and mill, shift in centrifuge tube, constant volume, mixing sample, centrifugal treating, collects supernatant in centrifuge tube, precipitate again successively
With phosphate buffer and distilled water flushing, precipitate C is obtained, collects all supernatants in centrifuge tube, constant volume, colorimetric method for determining five
Carbon sugar, hexose, record data；
S4：Liposoluble substance is removed, chloroform-methanol, vibration processing are added in precipitate C, centrifugal treating removes supernatant, sinks
Shallow lake is washed 1 time with 5.0ml methanol successively again, and 5ml acetone is washed 1 time, finally with distillation washing 1 time, is removed supernatant, is precipitated
D；
S5：Starch and assay are extracted, DMSO-H is added in precipitating in D2O is uniformly mixed, and concussion processing after centrifugation, is received
Collect supernatant, precipitation uses DMSO- H successively again2O and distilled water are rinsed respectively, are collected all supernatants, are precipitated
E, by supernatant constant volume, distilled water dialysis is collected liquid after all dialysis, is sampled, colorimetric method for determining pentose, hexose, note
Record data；
S6：KOH is added in precipitating in hemicellulose and colorimetric method assay in E, concussion processing, centrifugal treating collects supernatant
Liquid, precipitation use KOH and distilled water flushing, collect all supernatants successively again, obtain precipitation F, constant volume, five carbon of colorimetric method for determining
Sugar, hexose record data；
S7：H will be added in precipitation F in the insoluble hemicellulose of alkali and total fiber element and assay2SO4, mixing, concussion processing,
Distilled water constant volume, after cooling, centrifugal treating collects all supernatants, sampling, and colorimetric method for determining pentose, hexose record number
According to.
Preferably, the temperature of aeration cabinet is 45 DEG C in the S1, and drying time is 3-5 days, and the aperture of sieve is
0.5mm。
Preferably, it is 40 mesh that grit number is crossed in the S2, and the temperature of wind drying box is 40 DEG C, and drying to moisture is less than
10%。
Preferably, it is 40 mesh that grit number is crossed in the S2, and the temperature of wind drying box is 40 DEG C, and drying to moisture is less than
10%。
Preferably, the centrifugal treating carries out under 3000g rotating speeds, centrifugation time 5min.
Preferably, in described S4, S5, S6 and S7, vibration processing 150rpm in 25 °C of shaking tables is carried out,
The time of vibration processing is 1 hour in S4, S6 and S7, and the time of vibration processing is 12 hours in S5.
Having the beneficial effect that in the present invention：
Step of the present invention is close, improves the utilization rate of sample, is conducive to control experimental variable, improves the reliability of analysis result,
So that the analysis result of banana stalk chemical analysis is relatively reliable, be conducive to the utilization of banana stalk, rationally utilize resource.
Description of the drawings
Fig. 1 is a kind of flow diagram of the analysis method of banana stalk chemical analysis proposed by the present invention.
Specific implementation mode
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation describes, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.
Referring to Fig.1, a kind of analysis method of banana stalk chemical analysis, includes the following steps：
S1：Material prepares, and rubber sample is put into pond, is rinsed by tap water, chopping, in aeration cabinet at 45 DEG C
It is 3-5 days dry, moisture is recorded, sample is crushed with pulverizer, is sieved with sieve 0.5mm sieve pores, obtains Sample A, and will examination
Sample A is preserved for use at room temperature；
S2：Buck grinds Sample A, crosses 40 mesh sieve, and extraction obtains sample B, then through vacuum filter, and in aeration-drying
It is dry in case, it is dried to moisture at 40 DEG C and is less than 10%；
S3：Soluble sugar measures, and sample B is dried to weight under the conditions of 65 °C, mixing weighs 0.1000 g dry weight samples
Product, the 0.5M phosphate buffers that appropriate pH=7.0 are added fully are milled, and are shifted in 15 ml centrifuge tubes, constant volume to 10.0ml, are mixed
Even sample, 3000g centrifuge 5 min, collect supernatant in 50 ml centrifuge tubes, precipitation is washed with 5.0 ml buffer successively again
2 times, 5.0 ml distillations washing 2 times obtains precipitate C, collects all supernatants in 50 ml centrifuge tubes, constant volume, colorimetric method is surveyed
Determine pentose, hexose, records data；
S4：Liposoluble substance is removed, 5.0 ml chloroform-methanols are added in precipitate C（1：1 v/v）, in 25 °C of shaking tables
150rpm vibrates 1h, and 3000g centrifuges 5min, removes supernatant, and precipitation is washed 1 time with 5.0ml methanol successively again, and 5ml acetone is washed
1 time, finally with 5.0 ml distillations washing 1 time, supernatant is removed, precipitation D is obtained；
S5：Starch and assay are extracted, 5.0 ml DMSO-H are added in D in precipitating2O（9：1 v/v）, it is uniformly mixed, concussion
Processing, in 25 °C of shaking tables, 150rpm gently shaken overnights（12h）, after centrifugation, supernatant is collected, is precipitated again successively with 5.0 ml
DMSO- H2O is washed 2 times, and 5.0 ml distillations washing 3 times collects all supernatants, obtains precipitation E, supernatant is settled to
30.0ml, distilled water dialysis 48h（Bag filter 8000-14000Da every two hours changes a water）, collect liquid after all dialysis
Body is settled to 70.0ml in 100ml graduated cylinders, samples, colorimetric method for determining pentose, hexose；
S6：5.0 ml 4M KOH are added in precipitating in hemicellulose and colorimetric method assay in E（Include 1 mg/ml's
NaHB4）, 150rpm vibrates 1h in 25 °C of shaking tables, and 3000g centrifuges 5min, collects supernatant, precipitates again successively with 5.0 ml
4M KOH are washed 1 time, and 5 ml distillations washing 2 times collects all supernatants, obtains precipitation F, constant volume, five carbon of colorimetric method for determining
Sugar, hexose；
S7：3.0 ml 67% (v/v) H will be added in precipitation F in the insoluble hemicellulose of alkali and total fiber element and assay2SO4,
Mixing, 150rpm vibrates 1h in 25 °C of shaking tables, and distilled water is settled to 10.0ml（Terminate hydrolysis）, after cooling, 3000g from
Heart 5min collects all supernatants, sampling, colorimetric method for determining pentose, hexose, pentose be the insoluble hemicellulose of alkali, six
Carbon sugar is cellulose.
The several representative banana variety compositions of table 1
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto, and it is any
Those familiar with the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its invents
Design is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (6)
1. a kind of analysis method of banana stalk chemical analysis, which is characterized in that include the following steps：
S1：Material prepares, and banana stalk sample is put into pond, is rinsed by tap water, chopping, is done in aeration cabinet
It is dry, moisture is recorded, sample is crushed with pulverizer, sieved with sieve, obtains Sample A, and Sample A is preserved at room temperature and is waited for
With；
S2：Sample A is ground sieving by buck, and extraction obtains sample B, then through vacuum filter, and is done in aeration cabinet
It is dry；
S3：Soluble sugar measures, and sample B is dried to weight, mixing weighs dry weight sample, and appropriate phosphate buffer is added and fills
Divide and mill, shift in centrifuge tube, constant volume, mixing sample, centrifugal treating, collects supernatant in centrifuge tube, precipitate again successively
With phosphate buffer and distilled water flushing, precipitate C is obtained, collects all supernatants in centrifuge tube, constant volume, colorimetric method for determining five
Carbon sugar, hexose, record data；
S4：Liposoluble substance is removed, chloroform-methanol, vibration processing are added in precipitate C, centrifugal treating removes supernatant, sinks
Shallow lake is washed 1 time with 5.0ml methanol successively again, and 5ml acetone is washed 1 time, finally with distillation washing 1 time, is removed supernatant, is precipitated
D；
S5：Starch and assay are extracted, DMSO-H is added in precipitating in D2O is uniformly mixed, and concussion processing after centrifugation, is collected
Supernatant, precipitation use DMSO- H successively again2O and distilled water are rinsed respectively, collect all supernatants, obtain precipitation E,
By supernatant constant volume, distilled water dialysis is collected liquid after all dialysis, is sampled, colorimetric method for determining pentose, hexose, record
Data；
S6：KOH is added in precipitating in hemicellulose and colorimetric method assay in E, concussion processing, centrifugal treating collects supernatant
Liquid, precipitation use KOH and distilled water flushing, collect all supernatants successively again, obtain precipitation F, constant volume, five carbon of colorimetric method for determining
Sugar, hexose record data；
S7：H will be added in precipitation F in the insoluble hemicellulose of alkali and total fiber element and assay2SO4, mixing, concussion processing, steaming
Distilled water constant volume, after cooling, centrifugal treating collects all supernatants, sampling, and colorimetric method for determining pentose, hexose record number
According to.
2. a kind of analysis method of banana stalk chemical analysis according to claim 1, which is characterized in that lead in the S1
The temperature of wind drying box is 45 DEG C, and drying time is 3-5 days, and the aperture of sieve is 0.5mm.
3. a kind of analysis method of banana stalk chemical analysis according to claim 1, which is characterized in that mistake in the S2
Grit number is 40 mesh, and the temperature of wind drying box is 40 DEG C, and drying to moisture is less than 10%.
4. a kind of analysis method of banana stalk chemical analysis according to claim 1, which is characterized in that the S3 pilot scales
The drying temperature of sample B is 65 °C, and phosphate buffer is the 0.5M phosphate buffers of pH=7.0.
5. a kind of analysis method of banana stalk chemical analysis according to claim 1, which is characterized in that at the centrifugation
Reason carries out under 3000g rotating speeds, centrifugation time 5min.
6. a kind of analysis method of banana stalk chemical analysis according to claim 1, which is characterized in that the S4, S5,
In S6 and S7, vibration processing 150rpm in 25 °C of shaking tables is carried out, in S4, S6 and S7 the time of vibration processing be
1 hour, the time of vibration processing was 12 hours in S5.
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| CN201810884810.6A CN108732110A (en) | 2018-08-06 | 2018-08-06 | A kind of analysis method of banana stalk chemical analysis |
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| CN201810884810.6A CN108732110A (en) | 2018-08-06 | 2018-08-06 | A kind of analysis method of banana stalk chemical analysis |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113340764A (en) * | 2021-06-15 | 2021-09-03 | 中国林业科学研究院林产化学工业研究所 | Method for analyzing fiber fraction and fiber bundle content of plant fibers |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101187068A (en) * | 2007-02-15 | 2008-05-28 | 吴苏明 | Japanese banana fiber and its preparation method and uses |
-
2018
- 2018-08-06 CN CN201810884810.6A patent/CN108732110A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101187068A (en) * | 2007-02-15 | 2008-05-28 | 吴苏明 | Japanese banana fiber and its preparation method and uses |
Non-Patent Citations (5)
| Title |
|---|
| H. S. SHANTHA，ET AL.: "Accumulation of Starch in Banana Pseudostem and Fruit", 《JOURNAL OF FOOD SCIENCE》 * |
| LI KUN，ET AL.: "ANALYSIS OF THE CHEMICAL COMPOSITION AND MORPHOLOGICAL STRUCTURE OF BANANA PSEUDO-STEM", 《BIORESOURCES》 * |
| PCFLI4: "比色法测定可溶性糖、果胶、半纤维素、纤维素含量", 《HTTP://WWW.DOCIN.COM/P-701242522.HTML》 * |
| 吴莲蓉: "油菜茎秆生化成分和倒伏相关性研究", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
| 郑丽丽 等: "香蕉茎秆废弃物综合利用研究现状与分析 ", 《热带农业科学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113340764A (en) * | 2021-06-15 | 2021-09-03 | 中国林业科学研究院林产化学工业研究所 | Method for analyzing fiber fraction and fiber bundle content of plant fibers |
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Application publication date: 20181102 |