CN108721205B - Sustained-release multilayer dental implant and preparation method and evaluation method thereof - Google Patents
Sustained-release multilayer dental implant and preparation method and evaluation method thereof Download PDFInfo
- Publication number
- CN108721205B CN108721205B CN201810775133.4A CN201810775133A CN108721205B CN 108721205 B CN108721205 B CN 108721205B CN 201810775133 A CN201810775133 A CN 201810775133A CN 108721205 B CN108721205 B CN 108721205B
- Authority
- CN
- China
- Prior art keywords
- layer
- release
- implant
- parts
- mold
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
- A61K9/0024—Solid, semi-solid or solidifying implants, which are implanted or injected in body tissue
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/13—Amines
- A61K31/155—Amidines (), e.g. guanidine (H2N—C(=NH)—NH2), isourea (N=C(OH)—NH2), isothiourea (—N=C(SH)—NH2)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
- A61K47/38—Cellulose; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/02—Stomatological preparations, e.g. drugs for caries, aphtae, periodontitis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/02—Local antiseptics
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N3/00—Investigating strength properties of solid materials by application of mechanical stress
- G01N3/08—Investigating strength properties of solid materials by application of mechanical stress by applying steady tensile or compressive forces
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/15—Medicinal preparations ; Physical properties thereof, e.g. dissolubility
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2203/00—Investigating strength properties of solid materials by application of mechanical stress
- G01N2203/0014—Type of force applied
- G01N2203/0016—Tensile or compressive
- G01N2203/0019—Compressive
Abstract
The invention provides a slow-release multilayer dental implant and a preparation method and an evaluation method thereof. The implant comprises a first layer, a second layer and a third layer or is simplified into the first layer and the second layer, the three-dimensional size of the first layer is matched with the upper part of the hole, the thickness is more than 0.5mm, the three-dimensional size of the second layer is matched with the middle part of the hole, the thickness is more than 1.5mm, the third layer is conical, and a 0.1-0.5mL gap is reserved between the third layer and the bottom of the hole. The invention has the beneficial effects that: the multi-layer dental implant has good sealing property, proper mechanical strength, long-acting treatment effect and the like, does not use adhesive, and is convenient to take out after use; the preparation process is convenient, visual and adjustable, and is quick and cheap, and patients can try out the effect and adjust the surface morphology after taking the implant in the field; the provided in-vitro evaluation method of the multilayer dental implant can be used for optimizing the prescription process of the implant or supplementing expert design database information for doctors to retrieve.
Description
Technical Field
The invention belongs to the field of dental implants, and particularly relates to a slow-release multilayer dental implant as well as a preparation method and an evaluation method thereof.
Technical Field
Dental caries is a common disease in stomatology, is mainly treated by filling repair, and the whole treatment process comprises the following steps: the method comprises three steps of removing caries and forming a cavity, sterilizing and diminishing inflammation and permanently filling. Because the situation of the carious part is complex, the step of sterilizing and diminishing inflammation needs to last for 1 to 2 weeks, and the sterilizing and diminishing inflammation medicaments are required to be slowly released to play a long-acting role; also, the filling material is required to temporarily fill the cavity to prevent food waste from entering and ensure normal eating.
At present, manual medicine sealing is mostly adopted clinically. For example: filling the cavity with cotton ball or calcium hydroxide paste containing camphorol solution by manual method. However, the occlusive effect, mechanical strength and long-term treatment of the tampon and paste are not ideal. The following is likely to occur: 1) the filler falls off and food residues enter the cavity; 2) the medicine is soaked and eluted by saliva of the oral cavity, bitter taste of the oral cavity or toxic and side effects of gastrointestinal tracts appear, and the medicine concentration in the carious cavity is insufficient, so that the long-acting effect is avoided; 3) the filling part has foreign body sensation, and the filler moves during chewing to stimulate locally exposed dental nerves, resulting in pain. To date, there is no report that a sustained-release implant can be sterilized and temporarily filled in a cavity for a period of 1 to 2 weeks.
Disclosure of Invention
Based on the technical problems, the invention provides a slow-release multilayer dental implant and a preparation method and an evaluation method thereof. Provides a sustained-release implant required in the step of 'sterilization and inflammation diminishing' in the tooth cavity, and has the following characteristics: 1) the cavity can be completely sealed without an adhesive, and the cavity can be completely and smoothly taken out after 1-2 weeks of treatment; 2) the proper mechanical strength, surface and internal support can meet the pressure and adhesion of daily diet, and the design of the bottom cavity can further reduce the stimulation to the locally exposed dental nerve; 3) can load a plurality of medicaments to realize the long-acting treatment of the combination of the medicaments; the polymer carrier and the three-dimensional shape of each layer can be adjusted, or the type and the dosage of the release regulator are changed, the release behavior is further controlled and adjusted, the treatment concentration is quickly reached, and the treatment concentration is maintained for a long time.
The technical scheme for realizing the technical effects is as follows:
providing a slow release multi-layer dental implant, said implant comprising two parts, a first layer and a second layer, said first layer having a three-dimensional size matching an upper portion of a carious cavity and a height >0.5mm and being comprised of a plasticized polymer; the three-dimensional size of the second layer is matched with the middle part of the cavity of the decayed tooth, the height of the second layer is more than 1.5mm, and the second layer is composed of the following raw materials in parts by weight: 10-25 parts of medicine, 50-85 parts of plasticizing polymer and 5-25 parts of release regulator.
Preferably, the implant also comprises a third layer, wherein the third layer is conical, has a gap of 0.1-0.5mL with the bottom of the cavity, and consists of the following raw materials in parts by weight: 15-50 parts of medicine and 50-85 parts of plasticizing polymer.
Preferably, the plasticizing polymer in the raw material of the first layer is plasticized ethyl cellulose or plasticized cellulose acetate, the plasticizing polymer is prepared by adding 5-25 parts of plasticizer to 100 parts of ethyl cellulose or cellulose acetate polymer and mixing and plasticizing, and the plasticizer is triethyl citrate.
Preferably, the medicament in the second layer of raw material is chlorhexidine, calcium hydroxide or tetracycline, the plasticizing polymer is plasticized ethyl cellulose or plasticized cellulose acetate, and the release regulator is HPMC-E5 or HPMC-K100 LV; the plasticizing polymer is prepared by adding 5-25 parts of plasticizer into 100 parts of ethyl cellulose or cellulose acetate polymer, and mixing and plasticizing, wherein the plasticizer is triacetin, dibutyl sebacate or triethyl citrate.
Preferably, the drug in the third layer of raw material is chlorhexidine, calcium hydroxide, tetracyclines or ibuprofen, the plasticizing polymer is plasticized ethyl cellulose, plasticized cellulose acetate, plasticized polyvinyl alcohol or plasticized Soluplus, the plasticizing polymer is prepared by adding 5-25 parts of plasticizer into 100 parts of the polymers of ethyl cellulose, cellulose acetate, polyvinyl alcohol or Soluplus, and the plasticizer is triacetin, dibutyl sebacate or triethyl citrate.
The invention also provides a preparation method of the slow-release multilayer dental implant, which comprises the following steps:
s1: scanning a 3D oral cavity to obtain a three-dimensional model of a cavity in the dental caries, adjusting the height and the shape of each layer of an implant, designing the three-dimensional model of the implant, designing and preparing a three-dimensional model of a mold, and 3D printing 1/2 to prepare two molds;
s2: selecting the composition types of materials of each layer, and calculating the adding amount of the materials according to the volume of each layer;
s3: 1/2 preparing moulds are combined, each layer of material is respectively added into the preparing mould, hot pressing or mechanical pressing is carried out, 1/2 preparing moulds are broken, and the implant is obtained.
The conventional formula of each layer of raw materials can directly use a drug-containing carrier which is subjected to physical premixing or hot melt extrusion, the special formula can be prepared by temporary grinding and mixing, and a specification can be put in the special formula, so that the specific formula is described.
Preferably, the material for preparing the mold in step S1 is polylactic acid or acrylonitrile-butadiene-styrene copolymer, and the printing method is a fused deposition modeling method.
The invention also provides an evaluation method of the sustained-release multilayer dental implant, and specifically comprises a compression behavior evaluation method of the sustained-release multilayer dental implant and a drug release behavior evaluation method of the sustained-release multilayer dental implant.
The compression behavior evaluation method comprises the following steps: designing a compression mold with an internal cavity containing the decayed tooth and a plane bottom, 3D printing the compression mold, placing an implant sample in the cavity of the compression mold, vertically pressing down at a constant speed by adopting a spherical probe, drawing a stress-strain curve of the implant, and calculating mechanical parameters;
the evaluation method of the drug release behavior comprises the following steps: designing a release mold with an internal cavity containing the decayed tooth and an unclosed bottom, 3D printing the release mold, placing the release mold in a miniature release pool with the same size, adding a release medium into the miniature release pool, placing an implant sample in the cavity of the release mold, sampling at regular time to measure the concentration of the drug, drawing a drug release curve, slowly dripping the release medium on the upper surface of the implant sample, simulating the washing of saliva in the oral cavity, measuring the amount of the drug released from the top at regular time, and evaluating the bitter taste of the oral cavity or the toxic and side effect of the gastrointestinal tract.
Preferably, the material of the compression mold is polylactic acid or acrylonitrile-butadiene-styrene copolymer, the printing method is a fused deposition molding method, the diameter of the spherical probe is 1-5mm, and the pressing speed is 0.01-0.1 mm/s.
Preferably, the material of the release mold is polylactic acid or acrylonitrile-butadiene-styrene copolymer, the printing method is a fused deposition molding method, the dosage of the release medium in the micro release pool is 0.1-0.5mL, and the dropping speed of the slow dropping of the release medium on the upper surface of the implant is 0.01-0.05 mL/min.
The slow-release multilayer dental implant provided by the invention has the following beneficial effects:
(1) has good sealing property, proper mechanical strength and long-acting treatment effect. The implant is divided into a first layer, a second layer and a third layer. The three-dimensional size of the first layer is matched with the upper part of the cavity, the high mechanical strength of the material meets the requirement of surface strength for daily teeth, and the high plasticity of the material is favorable for ensuring the material to be matched with the cavity and improving the sealing effect; the three-dimensional size of the second layer is matched with the middle part of the hollow hole, and the second layer is mainly used for dispersing surface stress distribution, can meet daily stress and realize internal support under the condition of not using an adhesive; the carried medicine can be slowly released, and the medicine effect can be maintained for a long time; the third layer is conical, a gap of 0.1-0.5mL is formed between the third layer and the bottom of the cavity, and the gap is filled with physiological saline, so that the drug release can be accelerated, and the stimulation of mechanical pressure to internal nerves can be reduced; the conical shape has larger surface area, and the carried medicine can be quickly released to quickly reach the treatment concentration. As a simplification, the second and third layers of the implant can be combined in size and function, and the implant can be divided into two parts, a first layer and a second layer. Through the design of different materials and shapes of the layers, the implant can simultaneously have good sealing property, proper mechanical strength, long-acting treatment effect and the like.
The implant of the invention does not use adhesive, and has good sealing performance on the surface of the cavity during the use period of a patient (1-2 weeks), and does not influence normal chewing and eating; in addition, the inside of the cavity can also quickly reach the treatment concentration and continuously release the medicine, thereby achieving the effects of disinfection, bacteriostasis and the like. After the use is finished, the removal can be finished only by picking out with a sharp hook and simply washing, and the next step of permanent filling is carried out without additional polishing.
(2) The invention provides a preparation method of a slow-release multilayer dental implant. And (3) scanning the oral cavity in 3D to obtain a three-dimensional model of the carious cavity in the tooth, designing an implant model and a corresponding preparation model, and 3D printing the preparation mould. Because the preparation process is convenient, visual and adjustable, and is quick and cheap, the patient can take the implant on the spot, the trial effect is achieved and the surface morphology is adjusted. Based on the expert design platform, doctors can conveniently adjust the shape of the implant and the design of each layer of medicine according to the shape and the treatment requirements of the carious cavity, thereby meeting more patients.
(3) The invention provides a simulation in-vitro evaluation method of a slow-release multilayer dental implant. 3D printing a compression mold and a drug release mold containing corresponding cavities based on a three-dimensional model of the carious cavity in the tooth, and evaluating the compression behavior and the drug release behavior of the implant in the carious filling cavity. The results can be used to optimize the implant prescription process or to supplement expert design database information for review by the physician.
In conclusion, the design and preparation method of the implant meet the requirement of dental personalized medicine in the whole diagnosis, design, preparation and use processes.
Drawings
FIG. 1 is a typical preparation mold, compression mold and release mold appearance.
Figure 2 is an appearance of a typical three-layer implant.
Figure 3 is an appearance of a typical bi-layer implant.
Figure 4 is a stress-strain curve and before and after compression appearance for a trilayer implant.
FIG. 5 is a schematic (in cross-section) view of an in vitro release method.
Fig. 6 is an in vitro release profile of a typical implant.
FIG. 7 is a graph of the effect of plasticizer type on the mechanical properties of free films.
In the figure: A. preparing a mold set by using a round 1/2; B. preparing a set of moulds from the strip 1/2; C. a compression mold; D. releasing the mold; 1. an implant female die; 2. screw holes for combination; 3. an implant; 4. dropping a release medium; 5. releasing the mold; 6. a micro release tank; 7. the medium is released.
Detailed Description
The technical solution of the present invention is further specifically described by the following embodiments with reference to the accompanying drawings.
Example 1 preparation of a mold, Release mold, preparation of a compression mold
The method comprises the steps of obtaining decayed tooth information by adopting 3D scanning, designing a mold by SolidWorks software, taking polylactic acid as a material, and printing a corresponding preparation mold, a release mold and a pressed mold by adopting a fused deposition molding method. The diameter of the nozzle is 0.4mm, the printing temperature is 190 ℃, the printing speed is 45mm/s, and the layer height is 0.10 mm. FIG. 1 is a view of a typical preparation mold, release mold and compression mold appearance.
Example 2 a three-layer implant was prepared by the following specific steps:
s1: the preparation mold B of example 1 was selected.
S2: physically mixing 100 parts of ethyl cellulose and 15 parts of triethyl citrate, and uniformly grinding, wherein 80mg is taken as a first layer material; 10 parts of chlorhexidine, 61 parts of ethyl cellulose, 19 parts of glyceryl triacetate and 510 parts of HPMC-E, uniformly mixing, and performing hot melt extrusion (90 ℃, 60rpm, 10min) to obtain 50mg of the second layer material. 30 parts of chlorhexidine, 60 parts of polyvinyl alcohol and 10 parts of glyceryl triacetate, uniformly mixing, carrying out hot melt extrusion (85 ℃, 50rpm, 10min), and taking 50mg as a third layer material.
S3: and (3) combining 1/2 preparation molds, respectively adding the three layers of materials into the preparation molds, performing hot pressing (90 ℃, 5kg, 2min), and dividing the 1/2 preparation molds to obtain the three-layer implant. The appearance is shown in fig. 2.
Example 3 a bilayer implant was prepared by the following specific steps:
s1: and printing preparation molds with different forms aiming at different carious cavity three-dimensional models.
S2: 100 parts of cellulose acetate and 20 parts of triethyl citrate are physically mixed and uniformly ground, and 70mg, 90mg and 130mg of first-layer materials with the numbers 1-3 are respectively taken; 25 parts of chlorhexidine, 50 parts of cellulose acetate, 5 parts of triacetin and 520 parts of HPMC-E, uniformly mixing, carrying out hot melt extrusion (100 ℃, 60rpm, 10min), and respectively taking 20mg, 30mg and 80mg of the second layer with the number 1-3.
S3: combining 1/2 preparation moulds, respectively adding the two layers of materials into the preparation moulds for hot pressing (80 deg.C, 3kg, 3min), and dividing 1/2 preparation moulds to obtain 1# -3# double-layer implants with different shapes and sizes, and the appearance is shown in figure 3.
EXAMPLE 4 evaluation of compressive behavior of Tri-layer implants of example 2 of the invention
Designing a compression mold with a cavity in the dental caries and a plane bottom, 3D printing the compression mold, and placing the implant sample to be tested in the compression mold with the corresponding cavity. A spherical probe (diameter 3mm) was vertically pressed down at a constant speed (0.1mm/s), a stress-strain curve was plotted, and the mechanical strength was calculated. Figure 4 is a stress-strain curve and before and after compression appearance for a trilayer implant. The results show that: the implant is elastically deformed in the early stage and plastically deformed in the later stage, and the fracture stress of the implant is more than 28.26 MPa.
EXAMPLE 5 evaluation of the Release behavior of the three-layer implant of example 2 and the 3# bilayer implant of example 3 of the present invention
Designing a release mold with a cavity in the dental caries and an unclosed bottom, 3D printing the release mold, and placing in a miniature release tank with a consistent inner diameter (figure 5). 0.4mL of purified water is added into the micro release tank to be used as a release medium, the implant is placed in a release mold, samples are taken every 12h to determine the drug concentration, and a drug release curve is drawn. Purified water (0.05mL/min) was slowly added dropwise to the top of the implant to simulate oral saliva washing, the eluent was collected periodically, and the amount of drug released from the top was measured.
The results are shown in FIG. 6: both implants showed good sustained release performance over a one week period. The release rate and total release amount of the three-layer implant (the second layer contains 5mg of medicine and the third layer contains 15mg of medicine) are both larger than those of the 3# double-layer implant (the second layer contains 20mg of medicine). The possible reasons are: the third layer carrier of the three-layer implant is hydrophilic polymer, which is more beneficial to the release of the drug. Neither implant top surface eluent detected drug, indicating that the amount of drug released from the top was minimal.
Comparative study:
(1) effect of Polymer species on the mechanical Strength of the first layer of the implant
The fixing plasticizer was triethyl citrate, the amount of which was 7% of the amount of polymer, to prepare different plasticized polymers. The amount of the first layer was fixed to 50mg to prepare a double-layered implant. Soaking the implant in release medium water for 2h, taking out, wiping, putting into a corresponding compression mold, and vertically pressing with a spherical probe with diameter of 3 mm. The first layer of the implant was observed to deform under compression under a specified pressure. The results show that: when 5kg of pressure is applied, the first layer of the polymer, which is ethyl cellulose and cellulose acetate, has no obvious deformation and rupture, and the mechanical strength meets the requirement; the first layer, the polymer RL/RS, is slightly deformed, indicating poor mechanical strength.
(2) Effect of plasticizer type on Polymer mechanical Properties
The ethyl cellulose is fixed as a polymer, the dosage of the plasticizer is 20%, and the influence of the type of the plasticizer on the mechanical properties of the polymer is researched. The strands were hot-melted, hot-pressed to free films, and their mechanical properties were evaluated by stretching. The results of the experiment are shown in FIG. 7. When dibutyl sebacate is used as a plasticizer, the viscosity of an extruded strip is too high, and a flat and smooth free film cannot be prepared by hot pressing. Compared with glyceryl triacetate, the toughness and strength of the hot-pressed film are both obviously improved when triethyl citrate is used as a plasticizer (elongation at break is 10.0%, and strength limit is 3.40 MPa). Thus, the preferred plasticizer type for the first layer of the implant is triethyl citrate.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention in any way, and all simple modifications and equivalent variations of the above embodiments according to the technical spirit of the present invention are included in the scope of the present invention.
Claims (7)
1. A slow release multi-layer dental implant, characterized in that it comprises two parts, a first layer and a second layer, said first layer having a three-dimensional size coinciding with the upper part of the carious cavity and a height >0.5mm, and consisting of a plasticized polymer; the three-dimensional size of the second layer is matched with the middle part of the cavity of the decayed tooth, the height of the second layer is more than 1.5mm, and the second layer is composed of the following raw materials in parts by weight: 10-25 parts of medicine, 50-85 parts of plasticizing polymer and 5-25 parts of release regulator;
the plasticized polymer in the first layer of raw materials is plasticized ethyl cellulose or plasticized cellulose acetate, the plasticized polymer is prepared by adding 5-25 parts of plasticizer into 100 parts of ethyl cellulose or cellulose acetate polymer, and the plasticizer is triethyl citrate;
the medicine in the second layer of raw material is chlorhexidine, calcium hydroxide or tetracycline, the plasticizing polymer is plasticized ethyl cellulose or plasticized cellulose acetate, and the release regulator is HPMC-E5 or HPMC-K100 LV; the plasticizing polymer is prepared by adding 5-25 parts of plasticizer into 100 parts of ethyl cellulose or cellulose acetate polymer, and mixing and plasticizing, wherein the plasticizer is triacetin, dibutyl sebacate or triethyl citrate.
2. The slow-release multilayer dental implant according to claim 1, further comprising a third layer, wherein the third layer is conical, has a gap of 0.1-0.5mL with the bottom of the cavity, and is composed of the following raw materials in parts by weight: 15-50 parts of medicine and 50-85 parts of plasticizing polymer;
the third layer of the material contains chlorhexidine, calcium hydroxide, tetracyclines or ibuprofen, the plasticizing polymer is plasticized ethyl cellulose, plasticized cellulose acetate, plasticized polyvinyl alcohol or plasticized Soluplus, the plasticizing polymer is prepared by adding 5-25 parts of plasticizer into 100 parts of ethyl cellulose, cellulose acetate, polyvinyl alcohol or Soluplus polymer, and mixing and plasticizing, and the plasticizer is triacetin, dibutyl sebacate or triethyl citrate.
3. A process for the preparation of a sustained release multilayer dental implant according to claim 1 or 2, characterized in that it comprises in particular the following steps:
s1: scanning a 3D oral cavity to obtain a three-dimensional model of a cavity in the dental caries, adjusting the height and the shape of each layer of an implant, designing the three-dimensional model of the implant, designing and preparing a three-dimensional model of a mold, and 3D printing 1/2 to prepare two molds;
s2: selecting the composition types of materials of each layer, and calculating the adding amount of the materials according to the volume of each layer;
s3: 1/2 preparing moulds are combined, each layer of material is respectively added into the preparing mould, hot pressing or mechanical pressing is carried out, 1/2 preparing moulds are broken, and the implant is obtained.
4. The method of claim 3, wherein the mold is made of polylactic acid or acrylonitrile-butadiene-styrene copolymer in step S1, and the printing method is a fused deposition modeling method.
5. A method for evaluating the compression behavior and a method for evaluating the drug release behavior of the sustained-release multi-layered dental implant according to claim 1 or 2,
the compressive behavior evaluation method comprises the following steps: designing a compression mold with an internal cavity containing the decayed tooth and a plane bottom, 3D printing the compression mold, placing an implant sample in the cavity of the compression mold, vertically pressing down at a constant speed by adopting a spherical probe, drawing a stress-strain curve of the implant, and calculating mechanical parameters;
the method for evaluating the drug release behavior comprises the following steps: designing a release mold with an internal cavity containing the decayed tooth and an unclosed bottom, 3D printing the release mold, placing the release mold in a miniature release pool with the same size, adding a release medium into the miniature release pool, placing an implant sample in the cavity of the release mold, sampling at regular time to measure the concentration of the drug, drawing a drug release curve, slowly dripping the release medium on the upper surface of the implant sample, simulating the washing of saliva in the oral cavity, measuring the amount of the drug released from the top at regular time, and evaluating the bitter taste of the oral cavity or the toxic and side effect of the gastrointestinal tract.
6. The method for evaluating the compression behavior and the method for evaluating the drug release behavior of a sustained-release multi-layered dental implant according to claim 5, wherein the material of the compression mold is polylactic acid or acrylonitrile-butadiene-styrene copolymer, the printing method is a fused deposition modeling method, the diameter of the spherical probe is 1 to 5mm, and the pressing speed is 0.01 to 0.1 mm/s.
7. The method for evaluating the compression behavior and the drug release behavior of the sustained-release multi-layered dental implant according to claim 5, wherein the material of the release mold is polylactic acid or acrylonitrile-butadiene-styrene copolymer, the printing method is a fused deposition modeling method, the dosage of the release medium in the micro-release tank is 0.1-0.5mL, and the dropping speed of the slow dropping release medium on the upper surface of the implant is 0.01-0.05 mL/min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810775133.4A CN108721205B (en) | 2018-07-16 | 2018-07-16 | Sustained-release multilayer dental implant and preparation method and evaluation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810775133.4A CN108721205B (en) | 2018-07-16 | 2018-07-16 | Sustained-release multilayer dental implant and preparation method and evaluation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108721205A CN108721205A (en) | 2018-11-02 |
| CN108721205B true CN108721205B (en) | 2021-07-23 |
Family
ID=63926905
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810775133.4A Active CN108721205B (en) | 2018-07-16 | 2018-07-16 | Sustained-release multilayer dental implant and preparation method and evaluation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108721205B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3248649A1 (en) * | 1982-01-07 | 1983-07-14 | Nippon Kogaku K.K., Tokyo | DENTAL IMPLANT AND METHOD FOR THE PRODUCTION THEREOF |
| CN102038554A (en) * | 2011-01-06 | 2011-05-04 | 郑颖 | Pulp chamber decompression temporary stopping membrane |
| CN102170841A (en) * | 2008-08-26 | 2011-08-31 | 安迪·博扬久 | A dental bone implant, methods for implanting the dental bone implant and methods and systems for manufacturing dental bone implants |
| CN106668007A (en) * | 2017-01-20 | 2017-05-17 | 浙江工业大学 | Nidazole type oral-cavity sustained-release preparation and preparation method thereof |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5976507A (en) * | 1998-06-04 | 1999-11-02 | Colgate Palmolive Company | Dentrifice composition containing encapsulated reactive ingredients |
| JP6181655B2 (en) * | 2011-10-10 | 2017-08-16 | スリーエム イノベイティブ プロパティズ カンパニー | Airgel, baked and crystalline product and method for producing the same |
-
2018
- 2018-07-16 CN CN201810775133.4A patent/CN108721205B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3248649A1 (en) * | 1982-01-07 | 1983-07-14 | Nippon Kogaku K.K., Tokyo | DENTAL IMPLANT AND METHOD FOR THE PRODUCTION THEREOF |
| CN102170841A (en) * | 2008-08-26 | 2011-08-31 | 安迪·博扬久 | A dental bone implant, methods for implanting the dental bone implant and methods and systems for manufacturing dental bone implants |
| CN102038554A (en) * | 2011-01-06 | 2011-05-04 | 郑颖 | Pulp chamber decompression temporary stopping membrane |
| CN106668007A (en) * | 2017-01-20 | 2017-05-17 | 浙江工业大学 | Nidazole type oral-cavity sustained-release preparation and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| Controlled release of metronidazole from composit poly-caprolactone/alginate (PCL/alginate) rings for dental implants;Shih-Feng Lan et al.;《Dental Materials》;20131231;第29卷(第2013期);第656–665页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108721205A (en) | 2018-11-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Chen et al. | Pharmaceutical applications of 3D printing | |
| CN100408029C (en) | Composite dosage forms with a coating portion | |
| DE69635309T2 (en) | DENTAL IMPRESSION SPOON | |
| US20190133732A1 (en) | Dental appliance having ornamental design | |
| US20170325919A1 (en) | Method of making a denture | |
| US20180271620A1 (en) | Orthodontic appliances and methods of fabricating same | |
| KR101380630B1 (en) | Edentulous trays and disposable dentures made ^^using the same method | |
| Yang et al. | Fabrication and evaluation of dental fillers using customized molds via 3D printing technology | |
| CN106137416A (en) | Combined orthodontic system and manufacture method thereof | |
| Ardelean et al. | Manufacture of different types of thermoplastic | |
| CN108721205B (en) | Sustained-release multilayer dental implant and preparation method and evaluation method thereof | |
| US20200129421A1 (en) | Dental tray and use thereof | |
| CN106388964A (en) | Artificial tooth socket for slowly releasing medicament form | |
| EP0250578B1 (en) | Process for producing shapes for administering individual doses and shapes thus produced | |
| WO2017182425A1 (en) | Pharmaceutical dosage form mountable to a tooth | |
| Ou et al. | Personalized anesthetic patches for dental applications | |
| JP2018068628A (en) | Denture | |
| CN111298280B (en) | Soft back microneedle and manufacturing method thereof | |
| Gurkar et al. | Prosthodontic management of xerostomic patient: a technical modification | |
| CN107669648B (en) | 3D-printed doxofylline orally disintegrating tablet and preparation method thereof | |
| CN107811987B (en) | 3D printing aminophylline orally disintegrating tablet and preparation method thereof | |
| CN106137412A (en) | Can be used for facing of having a meal and preparation method thereof | |
| EP3068450A1 (en) | Tooth prosthesis as administration form for treating chronic diseases | |
| Singh et al. | Low volume salivary reservoir in complete denture prosthesis for xerostomia | |
| RU2311888C1 (en) | Method for producing double imprint when manufacturing stump inlays |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |