CN108719071A - A kind of rapid propagation method of Chinese rose of cut flower strain Q4 - Google Patents
A kind of rapid propagation method of Chinese rose of cut flower strain Q4 Download PDFInfo
- Publication number
- CN108719071A CN108719071A CN201810619835.3A CN201810619835A CN108719071A CN 108719071 A CN108719071 A CN 108719071A CN 201810619835 A CN201810619835 A CN 201810619835A CN 108719071 A CN108719071 A CN 108719071A
- Authority
- CN
- China
- Prior art keywords
- culture
- chinese rose
- cut flower
- rapid propagation
- propagation method
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
A kind of rapid propagation method of Chinese rose of cut flower strain Q4, the present invention relates to a kind of rapid propagation methods of Chinese rose of cut flower strain Q4, low, breeding cycle length problem that the purpose of the present invention is to solve traditional breeding method breeding coefficients, the present invention establishes Chinese rose of cut flower rapid propagation system by Initial culture, Multiplying culture, culture of rootage and rooting culture, breeding coefficient is improved, the breeding cycle is shortened.The present invention is applied to the breeding field of Chinese rose.
Description
Technical field
The present invention relates to a kind of rapid propagation methods of Chinese rose of cut flower strain Q4.
Background technology
Chinese rose is the woody flower of rose family Rosa.Chinese rose of cut flower is one of five big cut-flowers, cultivated area year by year on
Rise, the demand to improved seeds also goes up therewith.The advantage of the traditional cutting propagation mode of Chinese rose of cut flower is being kept in a short time
The fine quality of kind, but its breeding coefficient is low, according to branch length difference, is only capable of being divided into the stem of 5-10 length 10cm or so
Section, therefore can only at most breed 5-10 plant.The development trend of Chinese rose of cut flower industry is to commercially produce, and traditional breeding method is not
It can breed rapidly to reach batch production scale.
Invention content
The purpose of the present invention is to solve existing breeding Q4 strains method breeding coefficient is low, breeding cycle length is asked
Topic, provides a kind of rapid propagation method of Chinese rose of cut flower strain Q4.
A kind of rapid propagation method of Chinese rose of cut flower strain Q4 of the present invention carries out according to the following steps：
One, vegetable material is chosen：
The stem section in the middle part of the current-year branch that April or October take Chinese rose of cut flower strain Q4 growing way stalwartnesses, is cut into the small of 2-3cm
Section, every 1 segment carry 1 axillary bud, obtain explant；
Two, vegetable material sterilizes：
Explant 10min is impregnated with detergent, 10-20min is fully rinsed through flowing water, beaker after sterilizing is put into, uses volume
A concentration of 75% alcohol immersion 30s, aseptic water washing 2-4 times, after being transferred to volumetric concentration as 6.0%NaClO sterilizings 10min,
With aseptic water washing 4-6 times, explant after being sterilized；
Three, Multiplying culture：
After the dry tack free of explant after subject to sterilization, 1/2MS culture mediums are inoculated in, after cultivating 7-9d, carry out proliferation training
It supports；Proliferated culture medium adds plant growth regulator 6-BA 1.0mg/L+NAA 0.1mg/L, culture using MS as minimal medium
Light intensity is 2000Lux, and squamous subculture is carried out after cultivating 40d；Subculture medium adds plant growth tune using MS as minimal medium
Agent 6-BA 0.5mg/L+NAA 0.05mg/L, subculture cycle 40d are saved, subculture 3 times is chosen the tissue-cultured seedling of 2-3cm high, carried out
Culture of rootage；
Four, culture of rootage and rooting culture：
Culture of rootage is basic culture medium with 1/2MS, adds sucrose 20g/L, agar 6.0g/L, and add plant growth tune
Section agent IBA 0.5mg/L+NAA0.1mg/L inductions Q4 is taken root；The rooted seedling that selection plant height 4-5cm and root system stalwartness grow fine,
4-5d, then hardening 2-3d under conditions of relative humidity 75-85% are grown under the conditions of natural light, the transplantation of seedlings that will take root is to matrix
Transplanting is completed in middle continued growth.
The present invention establishes Q4 Chinese rose of cut flower rapid propagation systems, improves breeding coefficient, and rapid propagation system utilizes one
The stem section of a length 2-3cm can be proliferated 3-5 plant, and shorten the breeding cycle, can be completed within 46 days, be extensive quick
Breed batch production and technical support be provided, scientific basis is provided for merchandized handling, at the same be also Chinese rose of cut flower rearing new variety with
The foundation of genetic conversion system lays the foundation.
Description of the drawings
Fig. 1 is the Initial culture growing state in embodiment one；Wherein a is Q4 Initial cultures 0d；B is Q4 Initial cultures
5d；C is Q4 Initial cultures 8d；
Fig. 2 is the squamous subculture growing state in embodiment one；
Fig. 3 is the squamous subculture 40d squamous subculture growing states in embodiment one；
Fig. 4 is the situation of taking root in embodiment one；Wherein a is that Q4 is taken root 10d；B is that Q4 is taken root 15d；
Fig. 5 is that upgrowth situation is tamed in the transplanting in embodiment one.
Specific implementation mode
Technical solution of the present invention is not limited to the specific implementation mode of act set forth below, further include each specific implementation mode it
Between arbitrary combination.
Specific implementation mode one：A kind of rapid propagation method of Chinese rose of cut flower strain Q4 of present embodiment according to the following steps into
Row：
One, vegetable material is chosen：
The stem section in the middle part of the current-year branch that April or October take Chinese rose of cut flower strain Q4 growing way stalwartnesses, is cut into the small of 2-3cm
Section, every 1 segment carry 1 axillary bud, obtain explant；
Two, vegetable material sterilizes：
Explant 10min is impregnated with detergent, 10-20min is fully rinsed through flowing water, beaker after sterilizing is put into, uses volume
A concentration of 75% alcohol immersion 30s, aseptic water washing 2-4 times, after being transferred to volumetric concentration as 6.0%NaClO sterilizings 10min,
With aseptic water washing 4-6 times, explant after being sterilized；
Three, Multiplying culture：
After the dry tack free of explant after subject to sterilization, 1/2MS culture mediums are inoculated in, after cultivating 7-9d, carry out proliferation training
It supports；Proliferated culture medium adds plant growth regulator 6-BA 1.0mg/L+NAA 0.1mg/L, culture using MS as minimal medium
Light intensity is 2000Lux, and squamous subculture is carried out after cultivating 40d；Subculture medium adds plant growth tune using MS as minimal medium
Agent 6-BA 0.5mg/L+NAA 0.05mg/L, subculture cycle 40d are saved, subculture 3 times is chosen the tissue-cultured seedling of 2-3cm high, carried out
Culture of rootage；
Four, culture of rootage and rooting culture：
Culture of rootage is basic culture medium with 1/2MS, adds sucrose 20g/L, agar 6.0g/L, and add plant growth tune
Section agent IBA 0.5mg/L+NAA0.1mg/L inductions Q4 is taken root；The rooted seedling that selection plant height 4-5cm and root system stalwartness grow fine,
4-5d, then hardening 2-3d under conditions of relative humidity 75-85% are grown under the conditions of natural light, the transplantation of seedlings that will take root is to matrix
Transplanting is completed in middle continued growth.
Q4 strains Chinese rose of cut flower is commercial product in present embodiment.
Present embodiment establishes Q4 Chinese rose of cut flower rapid propagation systems, improves breeding coefficient, rapid propagation system profit
It can be proliferated 3-5 plant with the stem section of a length 2-3cm, and shorten the breeding cycle, can be completed within 46 days, be extensive
Quickly breeding batch production provides technical support, and scientific basis is provided for merchandized handling, while being also that Chinese rose of cut flower new varieties are trained
The foundation with genetic conversion system is educated to lay the foundation.
Specific implementation mode two：The present embodiment is different from the first embodiment in that：Detergent is body in step 2
The liquid detergent solution that product percentage concentration is 0.05%.Other are the same as one or two specific embodiments.
Specific implementation mode three：The present embodiment is different from the first and the second embodiment in that：Through flowing water in step 2
Fully rinse 15min.It is the same as one or two specific embodiments.
Specific implementation mode four：Unlike one of present embodiment and specific implementation mode one to three：Nothing in step 2
Bacterium water rinses 3 times.Other are identical as one of specific implementation mode one to three.
Specific implementation mode five：Unlike one of present embodiment and specific implementation mode one to four：It is trained in step 3
After supporting 8d, Multiplying culture is carried out.Other are identical as one of specific implementation mode one to four.
Specific implementation mode six：Unlike one of present embodiment and specific implementation mode one to five：Base in step 3
Matter is by vermiculite：Perlite：Turf=1：1：1 ratio mixes.Other are identical as one of specific implementation mode one to five.
Specific implementation mode seven：Unlike one of present embodiment and specific implementation mode one to six：Step 4 be
Hardening 2-3d under conditions of relative humidity 80%.Other are identical as one of specific implementation mode one to six.
Beneficial effects of the present invention are verified using following embodiment：
Embodiment one：A kind of rapid propagation method of Chinese rose of cut flower strain Q4 carries out according to the following steps：
One, vegetable material is chosen：
The stem section in the middle part of the current-year branch that October takes Chinese rose of cut flower strain Q4 growing way stalwartnesses, is cut into the segment of 2-3cm, often
1 segment carries 1 axillary bud, obtains explant；
Two, vegetable material sterilizes：
Explant 10min is impregnated with detergent, 15min is fully rinsed through flowing water, beaker after sterilizing is put into, uses volumetric concentration
30s is impregnated for 75% alcohol, aseptic water washing 3 times, after being transferred to volumetric concentration as 6.0%NaClO sterilizings 10min, use is sterile
Water rinses 5 times, explant after being sterilized；
Three, Multiplying culture：
After the dry tack free of explant after subject to sterilization, 1/2MS culture mediums are inoculated in, after cultivating 8d, carry out Multiplying culture；
Proliferated culture medium adds plant growth regulator 6-BA 1.0mg/L+NAA 0.1mg/L using MS as minimal medium, cultivates light
It is 2000Lux by force, squamous subculture is carried out after cultivating 40d, subculture medium adds plant growth regulating using MS as minimal medium
Agent 6-BA0.5mg/L+NAA 0.05mg/L, subculture cycle 40d, subculture 3 times choose the tissue-cultured seedling of 2-3cm high, take root
Culture；
Four, culture of rootage and rooting culture：
Culture of rootage is basic culture medium with 1/2MS, adds sucrose 20g/L, agar 6.0g/L, and add plant growth tune
Section agent IBA 0.5mg/L+NAA0.1mg/L inductions Q4 is taken root；The rooted seedling that selection plant height 4-5cm and root system stalwartness grow fine,
Grow 4-5d under the conditions of natural light, then the hardening 2-3d that is open under conditions of relative humidity 80%, the transplantation of seedlings that will take root is to base
Transplanting is completed in continued growth in matter.
Chinese rose of cut flower strain Q4 pollution rates change with materials Monthly changes.Q4 January pollution rate be 53.33%；In April
Pollution rate is 26.67%；July pollution rate be 36.67%；October pollution rate be 23.33%.The Q4 germinations of Chinese rose of cut flower strain
Rate changes with materials Monthly changes.Q4 January germination percentage be 46.67%；Q4 April germination percentage be 73.33%；Q4 is in July
Germination percentage is 63.33%；Q4 October germination percentage be 76.67%.The present embodiment is chosen the suitable time and is bred, and is being proliferated
Plant is higher close in the process, and young shoot growing way is healthy and strong.Add GA3It is unfavorable for proliferation and the growth of Q4 seedling.Cultivating light intensity is
2000Lux can make blade that green be presented.Subculture cycle is 40d, and growth coefficient highest is 3.10, and being proliferated again at this time can
Optimum state is in when keeping offspring to grow, while wound keeps relatively absorbing nutritional capacity by force.When the 3rd subculture, Q4 reaches
Highest growth coefficient 3.10.The Q4 radicals that root induction is suppressed, and induces in the NAA culture mediums of higher concentration compared with
It is few, therefore the NAA of suitable concentration is added in step 4, after transplanting seedling percent can 80% or more, seedling plants leaf it is green and
It grows fine.Fig. 1 is that Initial culture growing state germinates to form young leaves at Chinese rose Q4 stem segment with axillary buds as shown in Figure 1；Fig. 2 be after
It is commissioned to train foster growing state, Multiple Buds quantity increases during Multiplying culture as shown in Figure 2；Fig. 3 is squamous subculture 40d squamous subcultures
Shape is good in growing state, the Multiple Buds growth for pressing this patent culture medium prescription culture as shown in Figure 3；Fig. 4 is situation of taking root, can by figure
Root system is more sturdy when knowing 15d；Fig. 5 is transplanting domestication upgrowth situation, and as shown in Figure 5, the present embodiment can complete rooting culture mistake
Journey；
From the present embodiment it is found that the present embodiment growth coefficient is up to 3.10, compared with cuttage breeding method, the present embodiment is numerous
It is shorter (being shown in Table 1) to grow the period.
Table 1
| Cutting propagation mode | The present embodiment rapid propagation method | |
| The Initial culture time | 15 days | 8 days |
| The squamous subculture time (single) | 30 days or so | 30-40 days |
| The culture of rootage time | 15-20 days | 8 days |
| It is total | 60-65 days | 46-56 days |
In conclusion the present embodiment can be proliferated 3-4 plant using the stem section of a length 2-3cm, and shorten numerous
It grows the period, can be completed within 46 days, improve breeding coefficient, shorten proliferating cycle.The present embodiment establishes the Q4 strain cut-flower moons
Season rapid propagation system, improve breeding coefficient, shorten the breeding cycle, can be completed within 46 days, for extensive quickly breeding work
Factoryization provides technical support, and scientific basis is provided for merchandized handling, while being also that Chinese rose of cut flower rearing new variety and heredity turn
The foundation of change system lays the foundation.
Claims (7)
1. a kind of rapid propagation method of Chinese rose of cut flower strain Q4, it is characterised in that this method carries out according to the following steps：
One, vegetable material is chosen：
The stem section in the middle part of the current-year branch that April or October take Chinese rose of cut flower strain Q4 growing way stalwartnesses, is cut into the segment of 2-3cm,
Every 1 segment carries 1 axillary bud, obtains explant；
Two, vegetable material sterilizes：
Explant 10min is impregnated with detergent, 10-20min is fully rinsed through flowing water, beaker after sterilizing is put into, uses volumetric concentration
30s, aseptic water washing 2-4 times, after being transferred to volumetric concentration as 6.0%NaClO sterilizings 10min, with nothing are impregnated for 75% alcohol
Bacterium water rinses 4-6 times, explant after being sterilized；
Three, Multiplying culture：
After the dry tack free of explant after subject to sterilization, 1/2MS culture mediums are inoculated in, after cultivating 7-9d, carry out Multiplying culture；Increase
Culture medium is grown using MS as minimal medium, adds plant growth regulator 6-BA 1.0mg/L+NAA 0.1mg/L, cultivates light intensity
For 2000Lux, squamous subculture is carried out after cultivating 40d；Subculture medium adds plant growth regulator using MS as minimal medium
6-BA 0.5mg/L+NAA 0.05mg/L, subculture cycle 40d, subculture 3 times choose the tissue-cultured seedling of 2-3cm high, take root
Culture；
Four, culture of rootage and rooting culture：
Culture of rootage is basic culture medium with 1/2MS, adds sucrose 20g/L, agar 6.0g/L, and add plant growth regulator
IBA 0.5mg/L+NAA0.1mg/L inductions Q4 is taken root；The rooted seedling that selection plant height 4-5cm and root system stalwartness grow fine, certainly
4-5d, then hardening 2-3d under conditions of relative humidity 75-85% are grown under the conditions of right light, transplantation of seedlings to the matrix that will take root relays
Continuous growth, completes transplanting.
2. the rapid propagation method of Chinese rose of cut flower strain Q4 according to claim 1 a kind of, it is characterised in that in step 2
Detergent is the liquid detergent solution that concentration expressed in percentage by volume is 0.05%.
3. the rapid propagation method of Chinese rose of cut flower strain Q4 according to claim 1 a kind of, it is characterised in that in step 2
15min is fully rinsed through flowing water.
4. the rapid propagation method of Chinese rose of cut flower strain Q4 according to claim 1 a kind of, it is characterised in that in step 2
Aseptic water washing 3 times.
5. the rapid propagation method of Chinese rose of cut flower strain Q4 according to claim 1 a kind of, it is characterised in that in step 3
After cultivating 8d, Multiplying culture is carried out.
6. the rapid propagation method of Chinese rose of cut flower strain Q4 according to claim 1 a kind of, it is characterised in that in step 4
Matrix is by vermiculite：Perlite：Turf=1：1：1 ratio mixes.
7. the rapid propagation method of Chinese rose of cut flower strain Q4 according to claim 1 a kind of, it is characterised in that in step 4
It is hardening 2-3d under conditions of relative humidity 80%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810619835.3A CN108719071A (en) | 2018-06-14 | 2018-06-14 | A kind of rapid propagation method of Chinese rose of cut flower strain Q4 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810619835.3A CN108719071A (en) | 2018-06-14 | 2018-06-14 | A kind of rapid propagation method of Chinese rose of cut flower strain Q4 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108719071A true CN108719071A (en) | 2018-11-02 |
Family
ID=63929993
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810619835.3A Pending CN108719071A (en) | 2018-06-14 | 2018-06-14 | A kind of rapid propagation method of Chinese rose of cut flower strain Q4 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108719071A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109618928A (en) * | 2018-12-25 | 2019-04-16 | 江苏农林职业技术学院 | A method of reducing indoor pot Chinese rose tissue culture explant body pollution |
| CN111374057A (en) * | 2020-04-24 | 2020-07-07 | 黑龙江省科学院大庆分院 | Environment-friendly and efficient micro-propagation method for cold-resistant China roses |
-
2018
- 2018-06-14 CN CN201810619835.3A patent/CN108719071A/en active Pending
Non-Patent Citations (2)
| Title |
|---|
| 周艳等: "切花月季的快速繁殖技术", 《贵州农业科学》 * |
| 潘兵兵等: "耐寒丰花月季品系‘2004-6’组织培养快速繁殖的研究", 《中国观赏园艺研究进展（2010）》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109618928A (en) * | 2018-12-25 | 2019-04-16 | 江苏农林职业技术学院 | A method of reducing indoor pot Chinese rose tissue culture explant body pollution |
| CN111374057A (en) * | 2020-04-24 | 2020-07-07 | 黑龙江省科学院大庆分院 | Environment-friendly and efficient micro-propagation method for cold-resistant China roses |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103931492B (en) | The tissue culture fast seedling-cultivating method of apple rootstock M9 | |
| CN106942051B (en) | A kind of culture medium and propagation method of the tissue-culturing quick-propagation of alum root blade | |
| CN105815213A (en) | Establishing method for in-vitro regeneration system of Kiwi berry | |
| CN101695279B (en) | Method for tissue culture of populus deltoids forest 101 | |
| CN104885948B (en) | A kind of method of camellia oleosa seeds leaf segment directly regenerated plant | |
| CN108849503A (en) | A kind of rapid propagation method of Chinese rose of cut flower strain Q11 | |
| CN104221859B (en) | A kind of fast numerous cultural method of Garcinia mangostana | |
| CN105379624B (en) | A kind of tissue culture and rapid propagation method of Eucalyptus pellita | |
| CN113331059A (en) | Method for establishing efficient regeneration system by taking bird king tea tree hypocotyls as explants | |
| CN104604677A (en) | Tissue culture propagation method for reducing tissue culture browning rate of traxacum koksaghyz | |
| CN108719071A (en) | A kind of rapid propagation method of Chinese rose of cut flower strain Q4 | |
| CN101695280B (en) | Tissue culture and rapid propagation method of raspberries | |
| CN107568056A (en) | A kind of method of quick breeding apple Early blooming and fruiting stock | |
| CN104938335B (en) | The method that regeneration plant is obtained using oil tea hypocotyls | |
| CN104839019A (en) | Method for in-vitro rapid propagation of iris laevigata by using immature fruits | |
| CN112154916B (en) | Culture medium for culture and breeding method of lilac daphne flower bud explants and culture and breeding method of lilac daphne flower bud explants | |
| CN108739394A (en) | The quick breeding of Chinese rose of cut flower strain Q6 a kind of, regeneration method | |
| CN100358413C (en) | Cymbidium plant seed asepsis sprouting and plant cultivating method | |
| CN101904302B (en) | Method for somatic cell embryogeny and plant regeneration of medicinal plant schisandga chinensis baill | |
| CN101836589B (en) | Method of rapid propagation of populus | |
| CN102657098A (en) | In-vitro culture method for tender stem segments of cherry rootstock | |
| CN105379621A (en) | Efficient in-vitro plant regeneration method of adult high-quality single-plant Xiaoqiao oriental cherry of cerasus lannesiana var. speciosa | |
| CN109757231A (en) | A kind of method that Chinese yam idioplasm resource is quickly bred and saved | |
| CN104186324A (en) | Method for inducing mature zygotic embryo somatic cell embryos of pinus sylvestris var mongolica | |
| CN108719061B (en) | Method for inducing cherokee rose leaf to directly generate adventitious bud and regenerate plant |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20181102 |