CN108717092A - A kind of β value type variable high-speed counter-current chromatograph and its control method - Google Patents
A kind of β value type variable high-speed counter-current chromatograph and its control method Download PDFInfo
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- CN108717092A CN108717092A CN201810471007.XA CN201810471007A CN108717092A CN 108717092 A CN108717092 A CN 108717092A CN 201810471007 A CN201810471007 A CN 201810471007A CN 108717092 A CN108717092 A CN 108717092A
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- 238000000034 method Methods 0.000 title claims abstract description 18
- 230000005540 biological transmission Effects 0.000 claims abstract description 17
- 230000033001 locomotion Effects 0.000 claims abstract description 15
- 230000001360 synchronised effect Effects 0.000 claims description 14
- 230000035515 penetration Effects 0.000 claims description 10
- 230000008878 coupling Effects 0.000 claims description 7
- 238000010168 coupling process Methods 0.000 claims description 7
- 238000005859 coupling reaction Methods 0.000 claims description 7
- 238000001514 detection method Methods 0.000 claims description 3
- 230000005611 electricity Effects 0.000 claims 1
- 230000008859 change Effects 0.000 abstract description 3
- 239000003814 drug Substances 0.000 abstract description 3
- 238000000926 separation method Methods 0.000 abstract description 2
- 229940079593 drug Drugs 0.000 abstract 2
- 238000010262 high-speed countercurrent chromatography Methods 0.000 description 6
- 238000009792 diffusion process Methods 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000002411 adverse Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000010276 construction Methods 0.000 description 1
- 238000003891 environmental analysis Methods 0.000 description 1
- 239000000686 essence Substances 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 238000004810 partition chromatography Methods 0.000 description 1
- 238000004804 winding Methods 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/38—Flow patterns
- G01N30/42—Flow patterns using counter-current
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a kind of β value（The ratio of splitter rotation radius and revolution-radius）Type variable high-speed counter-current chromatograph and its control method.Including control system, industrial personal computer, motion software, central shaft driven system, attached planet shaft transmission system, babinet, hose, feed and exhaust tube.The central shaft driven system is fixed on by support plate on babinet, the attached planet shaft transmission system is connect by planet carrier with central shaft driven system, the feed and exhaust tube is connect across central shaft with hose, the hose is connect with the splitter in attached planet axis, drug passes in and out splitter by feed and exhaust tube and hose, the rotation of the control system control motor, drive central shaft driven system and the operating of attached planet shaft transmission system, realize the separation of drug in splitter, the control system is controlled by motion software, and the motion software is mounted on industrial personal computer.The distance between attached planet axis can be arranged in the present invention, the change of β value size is realized, in order to analyze influence of the different β value conditions to separating degree.
Description
Technical field
The invention belongs to high-speed countercurrent chromatography field, more particularly to a kind of β value（Splitter rotation radius and revolution half
The ratio of diameter）Type variable high-speed counter-current chromatograph and its control method.Splitter can be radially slided with sliding block along planet carrier, from
And realize the change of β value, to study influence of the β value to medical separation effect.
Background technology
High-speed countercurrent chromatography (high speed countercurrent chromatography, abbreviation HSCCC)
It is the new chromatographic technology developed by U.S. National Institutes doctor Ito, can realizes that liquid-liquid is efficiently divided in a short time
From and prepare, and thousands of a theoretical cam curves can be reached.High-speed countercurrent chromatography is a kind of New-of continuous high-efficient
Liquid partition chromatography technology, it is not necessarily to any solid-state carrier or support.In recent years, HSCCC relies on it significant in isolation technics
Advantage is widely used to the multiple fields such as Chinese medicine, biochemistry, food, natural product chemistry, environmental analysis, becomes China's modern times
The mainstay of science and technology and economic construction.
But just at present both at home and abroad from the point of view of the present Research in the field, needs to analyze and solve there are still many problems.Example
Research such as is lacked to the influence factor of sample diffusion in adverse current chromatogram；Separating capacity and efficiency are low；The column effect of shortage system is theoretical,
It cannot instruct equipment improvement etc..Wherein, it is to improve high-speed counter-current color to solve sample diffusion in adverse current chromatogram from mechanical design aspect
Compose the key of column effect.Such as the axis of rotation and influence of the hollow shaft angle to sample diffusion；Tubing internal diameter, shape, length and winding
Influence of the angle to sample diffusion；Influence etc. of the change of β value to sample diffusion.
Invention content
It is an object of the invention to：In view of the above problems, provide a kind of β value type variable high-speed counter-current chromatograph and
Its control method.The splitter of the high-speed counter-current chromatograph can be moved radially with sliding block along track, to realize changing for β value
Become.
The technical proposal of the invention is realized in this way：β value type variable high-speed counter-current chromatograph and its control method, it is special
Sign is：Including control system, industrial personal computer（31）, motion software（30）, central shaft driven system, attached planet shaft transmission system,
Babinet（1）, hose（19）, feed and exhaust tube（24）, control interface（2）, the central shaft driven system passes through support plate（4）It is fixed
In babinet（1）On, the attached planet shaft transmission system is connect with central shaft driven system, and the control system controls motor（3）
Rotation, the control system is by control interface（2）Control.
β value type variable high-speed counter-current chromatograph of the present invention, it is characterised in that：The central shaft driven system includes
Motor, motor fixing plate, V-type band, planet carrier, central shaft, penetration pipe, shaft coupling, support plate.
β value type variable high-speed counter-current chromatograph of the present invention, the central shaft driven system, it is characterised in that：Institute
It states motor threadably to fix on the supporting plate by motor fixing plate, the motor is connect by V-type band with planet carrier, institute
It states planet carrier to connect by deep groove ball bearing with central shaft, the central shaft is fixed on the supporting plate by shaft coupling, described to wear
Spool is threadedly fixed on center shaft, and the support plate is threadedly fixed on babinet.
β value type variable high-speed counter-current chromatograph of the present invention, the attached planet shaft transmission system include sliding block, attached row
Star axis, attached planet bracing strut, attached planet axis synchronous pulley, splitter, the sliding block are threadedly fixed on the planet carrier,
The attached planet bracing strut is threadedly fixed on sliding block, and the attached planet axis is connected with sliding block by deep groove ball bearing
It connects, the attached planet axis is connect with attached planet bracing strut by deep groove ball bearing, and the attached planet axis synchronous pulley passes through attached row
Star axis synchronous pulley flat key is fixed in attached planet axis, and the splitter is threadedly connect with attached planet axis, described attached
Planet shaft transmission system is slided by sliding block on planet carrier, is changed the revolution-radius of splitter and is realized the variation of β value.
β value type variable high-speed counter-current chromatograph of the present invention, described hose one end is fixed on by special spline wears
Spool, the other end are fixed on by hose clamping rings on splitter.
β value type variable high-speed counter-current chromatograph of the present invention, the feed and exhaust tube are fixedly arranged in hose, and one section passes through
Interface is connect with splitter, and the other end passes through penetration pipe and central shaft threadedly to be connect with external detection equipment.
β value type variable high-speed counter-current chromatograph of the present invention, the control interface are threadedly fixed on case
On body.
β value type variable high-speed counter-current chromatograph of the present invention and its control method, it is characterised in that：Mainly by controlling
System, motion software and industrial personal computer driving β value type variable high-speed counter-current chromatograph are operated, and the control system includes movement
Control system, temperature control system, vibration control system, speed control system.
β value type variable high-speed counter-current chromatograph of the present invention and its control method, the motion software are mounted on work
In control machine, corresponding instruction is set in industrial personal computer, command control system executes corresponding function.
β value type variable high-speed counter-current chromatograph of the present invention and its control method, the kinetic control system is by finger
It enables and control motor, motor driving v-belt is required to drive planet carrier around central shaft high speed rotation.
β value type variable high-speed counter-current chromatograph of the present invention and its control method, the temperature control system pass through
Temperature in temperature sensor control cabinet body, the babinet keep a certain steady temperature under temperature control system.
β value type variable high-speed counter-current chromatograph of the present invention and its control method, the vibration control system pass through
Vibrating sensor controls the vibration of babinet, and the babinet keeps lower oscillating region under vibration control system.
β value type variable high-speed counter-current chromatograph of the present invention and its control method, the speed control system pass through
The rotating speed of speed sensorless control motor, the motor reach the rotating speed requirement of setting under speed control system.
Description of the drawings
Fig. 1 is the complete machine structure schematic diagram of the present invention.
Fig. 2 is the machine driven system structural schematic diagram of the present invention.
Fig. 3 is the main axis transmission system sectional view of the present invention.
Fig. 4 is the attached planet shaft transmission system sectional view of the present invention.
Fig. 5 is motion control schematic diagram
Reference numeral：1 is babinet, and 2 panels in order to control, 3 be motor, and 4 be support plate, and 5 be motor fixing plate, and 6 be V-type band, and 7 are
V-type belt wheel, 8 be sliding block, and 9 be shaft coupling, and 10 be planet carrier, and 11 be tension pulley lever, and axis synchronous pulley centered on 12,13 are
Double cog synchronous belt, 14 be hose clamping rings, axis synchronous pulley flat key centered on 15, axis centered on 16, and 17 be penetration pipe, 18
For tensioning wheel, 19 be hose, and 20 be interface, and 21 be splitter, and 22 be attached planet shaft supporting frame, and 23 be electric machine main shaft, 24 for into
Discharge nozzle, 25 be shaft end ring, and 26 be deep groove ball bearing, and 27 be attached planet axis, and 28 be attached planet axis synchronous pulley, and 29 be attached row
Star axis synchronous pulley flat key, 30 be motion software, and 31 be industrial personal computer, and 32 be kinetic control system, and 33 be temperature control system, 34
It is speed control system for vibration control system, 35.
Specific implementation method
Below in conjunction with the accompanying drawings, the present invention is described in detail.
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to the accompanying drawings and embodiments, right
The present invention is further elaborated, it should be understood that and the specific embodiments described herein are merely illustrative of the present invention, and
It is not used in the restriction present invention.
As shown in Fig. 1,2,3,4 and 5, β value type variable high-speed counter-current chromatograph and its control method, including control system,
Industrial personal computer 31, motion software 30, central shaft driven system, attached planet shaft transmission system, babinet 1, hose 19, feed and exhaust tube 24,
Control panel 2, the central shaft driven system are fixed on by support plate 4 on babinet 1, and the attached planet shaft transmission system is in
Spindle drive system connects, and the rotation of the control system control motor 3, the control system is controlled by control interface 2.
Wherein, the central shaft driven system includes motor 3, motor fixing plate 5, V-type band 6, planet carrier 10, central shaft
16, penetration pipe 17, shaft coupling 9, support plate 4.The motor 3 is threadably fixed on support plate 4 by motor fixing plate 5
On, the motor 3 is connect by V-type band 6 with planet carrier 10, and the planet carrier 10 is connected with central shaft 16 by deep groove ball bearing 26
It connects, the central shaft 16 is fixed on by shaft coupling 9 in support plate 4, and the penetration pipe 17 is threadedly fixed on center
On axis 16, the support plate 4 is threadedly fixed on babinet.
Wherein, the attached planet shaft transmission system includes sliding block 8, attached planet axis 27, attached planet bracing strut 22, attached planet axis
Synchronous pulley 28, splitter 21, the sliding block 8 are threadedly fixed on planet carrier 10, the attached planet bracing strut 22
It is threadedly fixed on sliding block 8, the attached planet axis 27 is connect with sliding block 8 by deep groove ball bearing 26, the attached row
Star axis 27 is connect with attached planet bracing strut 22 by deep groove ball bearing 26, and the attached planet axis synchronous pulley 28 passes through attached planet axis
Synchronous pulley flat key 29 is fixed in attached planet axis 27, and the splitter 21 is threadedly connect with attached planet axis 27.Institute
It states attached planet shaft transmission system to slide on planet carrier 10 by sliding block 8, changes the revolution-radius of splitter 21 and realize β value
Variation.
As shown in Fig. 2, 19 one end of the hose is fixed on penetration pipe 17 by special spline, the other end is fixed by hose
Annulus 14 is fixed on splitter 21.
As shown in figure 3, the feed and exhaust tube 24 is fixedly arranged in hose 19, one section is connect by interface 20 with splitter 21,
The other end passes through penetration pipe 17 and central shaft 16 threadedly to be connect with external detection equipment.
As shown in figure 5, β value type variable high-speed counter-current chromatograph and its control method, mainly by control system, motion software
30 and industrial personal computer 31 drive β value type variable high-speed counter-current chromatograph operated, the control system includes kinetic control system
32, temperature control system 33, vibration control system 34, speed control system 35.
Wherein, the motion software 30 is mounted in industrial personal computer 31, and corresponding instruction, order control are arranged in industrial personal computer 31
System processed executes corresponding function.
As shown in Fig. 2, the kinetic control system 32 controls motor 3 by instruction requirement, 3 driving v-belt 6 of motor drives row
Carrier 10 is around 16 high speed rotation of central shaft.
Wherein, the temperature control system 33 controls the temperature in babinet 1 by temperature sensor, and the babinet 1 is in temperature
Spend 33 times a certain steady temperatures of holding of control system.
Wherein, the vibration control system 34 controls the vibration of babinet 1 by vibrating sensor, and the babinet 1 is vibrating
34 times lower oscillating regions of holding of control system.
Wherein, the speed control system 35 is by the rotating speed of speed sensorless control motor 3, and the motor 3 is in speed
Reach the rotating speed requirement of setting under control system 35.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
All any modification, equivalent and improvement etc., should all be included in the protection scope of the present invention made by within refreshing and principle.
Claims (7)
1. a kind of β value type variable high-speed counter-current chromatograph and its control method, it is characterised in that：Including control system, industrial personal computer
（31）, motion software, central shaft driven system, attached planet shaft transmission system, babinet（1）, hose（19）, feed and exhaust tube（24）,
Control interface（2）, the central shaft driven system is fixed on babinet by support plate（1）On, the attached planet shaft transmission system
It is connect with central shaft driven system, the control system controls motor（3）Rotation, the control system is by control interface（2）
Control.
2. β value type variable high-speed counter-current chromatograph according to claim 1, it is characterised in that：The central shaft driven system
System includes motor（3）, motor fixing plate（5）, V-type band（6）, planet carrier（10）, central shaft（16）, penetration pipe（17）, shaft coupling
（9）, support plate（4）.
3. central shaft driven system according to claim 2, it is characterised in that：The motor（3）Threadably pass through electricity
Machine fixed plate（5）It is fixed on support plate（4）On, the motor（3）Pass through V-type band（6）With planet carrier（10）Connection, the planet
Frame（10）With central shaft（16）Pass through deep groove ball bearing（26）Connection, the central shaft（16）Pass through shaft coupling（9）It is fixed on branch
Fagging（4）On, the penetration pipe（17）Threadedly it is fixed on central shaft（16）On, the support plate（4）Pass through screw thread
Mode is fixed on babinet（1）On.
4. β value type variable high-speed counter-current chromatograph according to claim 1, it is characterised in that：The attached planet axis transmission
System includes sliding block（8）, attached planet axis（27）, attached planet bracing strut（22）, attached planet axis synchronous pulley（28）, splitter
（21）, the sliding block（8）Threadedly it is fixed on planet carrier（10）On, the attached planet bracing strut（22）By screw thread side
Formula is fixed on sliding block（8）On, the attached planet axis（27）With sliding block（8）Pass through deep groove ball bearing（26）Connection, the attached planet
Axis（27）With attached planet bracing strut（22）Pass through deep groove ball bearing（26）Connection, the attached planet axis synchronous pulley（28）By attached
Planet axis synchronous pulley flat key（29）It is fixed on attached planet axis（27）On, the splitter（21）With attached planet axis（27）Pass through spiral shell
Line mode connects, and the attached planet shaft transmission system passes through sliding block（8）In planet carrier（10）Upper sliding changes splitter（21）'s
Revolution-radius and the variation for realizing β value.
5. β value type variable high-speed counter-current chromatograph according to claim 1, it is characterised in that：The feed and exhaust tube（24）
It is fixedly arranged at hose（19）In, one end passes through interface（20）With splitter（21）Connection, the other end pass through penetration pipe（17）The center and
Axis（16）Threadedly it is connect with external detection equipment.
6. β value type variable high-speed counter-current chromatograph control method, it is characterised in that：Mainly by control system, motion software（30）
And industrial personal computer（31）Driving β value type variable high-speed counter-current chromatograph is operated, and the control system includes kinetic control system
（32）, temperature control system（33）, vibration control system（34）, speed control system（35）.
7. β value type variable high-speed counter-current chromatograph control method according to claim 6, it is characterised in that：The movement
Control system（32）Motor is controlled by instruction requirement（3）, motor（3）Driving v-belt（6）Drive planet carrier（10）Around central shaft
（16）High speed rotation, the temperature control system（33）Babinet is controlled by temperature sensor（1）Interior temperature, the babinet
（1）In temperature control system（33）It is lower to keep a certain steady temperature, the speed control system（35）Pass through velocity sensor control
Motor processed（3）Rotating speed, the motor（3）In speed control system（35）Under reach the rotating speed requirement of setting.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810471007.XA CN108717092B (en) | 2018-05-17 | 2018-05-17 | Beta value variable high-speed countercurrent chromatograph and control method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810471007.XA CN108717092B (en) | 2018-05-17 | 2018-05-17 | Beta value variable high-speed countercurrent chromatograph and control method thereof |
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| CN108717092A true CN108717092A (en) | 2018-10-30 |
| CN108717092B CN108717092B (en) | 2024-04-05 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110960886A (en) * | 2019-11-11 | 2020-04-07 | 四川大学 | High-speed counter-current chromatographic separation column |
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Cited By (1)
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| CN110960886A (en) * | 2019-11-11 | 2020-04-07 | 四川大学 | High-speed counter-current chromatographic separation column |
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