CN108714219A - A kind of pea protein-curcumin nano compound and its preparation method and application - Google Patents
A kind of pea protein-curcumin nano compound and its preparation method and application Download PDFInfo
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Abstract
The present invention relates to a kind of pea protein-curcumin nano compounds and its preparation method and application.The weight ratio of pea protein and curcumin is 25 in compound:1-60:1.The preparation method includes step：(1) it weighs a certain amount of pea protein to be scattered in distilled water or deionized water, stirs lower homogeneous, then carry out Micro Fluid processing, be then centrifuged for, collect suspension and the suspension is lyophilized and obtain pea protein powder；(2) using the condition of the pea protein powder and curcumin optimization Micro Fluid method, best Micro Fluid condition is chosen；(3) according to the best Micro Fluid condition in step (2), pea protein-curcumin nano compound lotion is prepared using Micro Fluid method, the lotion is then freeze-dried and prepares pea protein-curcumin nano composite powder.The dispersion efficiency and the concentration in pea protein-curcumin nano mixed emulsion that the compound is greatly improved curcumin, can be used for preparing antitumor, immunological regulation, anti-oxidant or Antiatherosclerosis medicine.
Description
Technical field
The present invention relates to a kind of soluble curcumin nano compounds, more specifically to a kind of pea protein-turmeric
Plain nano-complex and its preparation method and application.
Background technology
Curcumin is a kind of natural pigment extracted from turmeric, in recent years because curcumin is antitumor, immunological regulation, resists
The action function of oxidation and antiatherosclerosis etc., causes the extensive concern of domestic and foreign scholars.However, due to curcumin
Poorly water-soluble is not easy by body absorption, is metabolized again quickly, so that its bioavilability is very low.Curcumin is to light, heat, acid
Environmental factor is more sensitive, and the storage stability for improving curcumin is food and medical industry focus of attention all the time.It passes
The method that system improves curcumin stability mainly has solvent deposition method and microemulsion investment etc..Solvent deposition method mainly passes through
Curcumin is dissolved in the organic solvents such as ethyl alcohol or DMSO, is reloading in liposome, polysaccharide, starch, EGCG, PEG or PLGA
In equal polymer supports, then by solvent precipitation prepare curcumin solid dispersed complex (Li et al., 2018；Sun,
Tian,Chen,&Jin,2017；Shah et al.,2018；Mahmood et al.,2018；Abdel-Hafez,
Hathout,&Sammour,2018；Arya,Das,&Sahoo,2018).However solvent deposition method have curcumin waste it is serious,
The defects of preparation process complexity and low output.Microemulsion method wraps up curcumin and applies in recent years than wide, mainly passes through W/O
Or the modes such as O/W wrap up curcumin, with improve curcumin dissolubility or bioactivity (Kharat, Zhang , &McClement,
2018；Zheng et al.,2017；Raviadaran,Chandran,Shin,&Manickam,2018).However this method has
There is the defects of turmeric cellulose content is low and emulsifier safety is poor.In addition to this, micro capsule technology can also improve the water of curcumin
Dissolubility and storage stability, however since many wall materials itself are easy to happen flocculation, mistake of some curcumin microcapsules in storage
Precipitation has just been formd in journey, make it difficult to be applied in industrial production (Ko et al., 2015；Liu,Chen,Cheng,&
Selomulya,2016；Paramera,Konteles,&Karathanos,2011).Therefore, one kind there is no suitably may be used at present
In the method for stable dispersion curcumin.
Dispersing method of the curcumin in pea protein aqueous solution is improved by Micro Fluid in patent of the present invention.Pea protein
It is because pea protein contains distinctive thiol bond, amino bond and carboxyl key that can be combined with curcumin under Micro Fluid condition of high voltage
--- --- instantaneous mixing --- --- polymerization is reacted, to efficiently prepare pea protein-turmeric Deng can disperse with curcumin
Plain solid dispersion.
Invention content
In view of the deficiencies in the prior art, the first object of the present invention is to provide a kind of pea protein-curcumin and receives
Curcumin is dispersed in pea protein aqueous solution by microfluidization and is prepared by rice compound, the compound, this is compound
Object greatly reduces the crystallinity of curcumin, improves the thermal stability and dispersibility in water of curcumin.
It is a further object of the present invention to provide a kind of preparation methods of pea protein-curcumin nano compound.This side
Method has many advantages, such as that operating condition is mild, easy to operate and can be prepared on a large scale.
Still a further object of the present invention is to provide a kind of compound comprising pea protein-curcumin nano compound and water.
Still a further object of the present invention is to provide a kind of purposes of pea protein-curcumin nano compound.
For above-mentioned purpose, as the first aspect of the present invention, the present invention provides a kind of pea protein-curcumin nanos
The weight ratio of compound, wherein pea protein and curcumin is 25:1-60:1.
In the more preferable embodiment of the present invention, the weight ratio of pea protein and curcumin is 25:1.In this hair
In another bright more preferable embodiment, the weight ratio of pea protein and curcumin is 35:1.The present invention another more
In preferred embodiment, the weight ratio of pea protein and curcumin is 50:1.In another more preferable embodiment of the present invention
In, the weight ratio of pea protein and curcumin is 60:1.
In a preferred embodiment of the present invention, the grain size of pea protein-curcumin nano compound is 50-
75nm.In another more preferable embodiment of the present invention, pea protein-curcumin nano compound grain size is 50-60nm.
In a preferred embodiment of the present invention, the polydispersity of pea protein-curcumin nano compound is
0.13-0.25.In another more preferable embodiment of the present invention, the polydispersion of pea protein-curcumin nano compound
Degree is 0.15-0.25.
In a preferred embodiment of the present invention, the surface tension of pea protein-curcumin nano compound is 40-
47mN/m.In another more preferable embodiment of the present invention, the surface tension of pea protein-curcumin nano compound is
40-45mN/m。
In a preferred embodiment of the present invention, the Zeta- current potentials of pea protein-curcumin nano compound be-
33--40mV.In the more preferable embodiment of the present invention, the Zeta- current potentials of pea protein-curcumin nano compound
For -35--38mV.
In the more preferable embodiment of the present invention, the grain size of the pea protein is 50-70nm.
In the more preferable embodiment of the present invention, the pea protein is to handle to purify by Micro Fluid.
As the second aspect of the present invention, the present invention provides a kind of preparations of pea protein-curcumin nano compound
Method, this approach includes the following steps：
(1) purifying (removal of impurities) of pea protein：A certain amount of pea protein is weighed to be scattered in distilled water or deionized water,
Under agitation, homogeneous 10-30min, then carry out Micro Fluid method processing, the pea protein lotion after having handled carry out from
The heart collects suspension and the suspension is lyophilized obtains pea protein powder；
(2) condition of optimization Micro Fluid processing pea protein-curcumin nano lotion：The pea obtained using step (1)
Protein powder and curcumin optimization Micro Fluid method prepare the condition of pea protein-curcumin, choose best Micro Fluid condition；
(3) it according to the best Micro Fluid condition in step (2), prepares pea protein-curcumin using Micro Fluid method and receives
Then rice milk liquid is freeze-dried the lotion and prepares pea protein-curcumin nano composite powder.
In a preferred embodiment of the present invention, pea protein and distilled water or the use of deionized water in step (1)
Amount is than being 3-6:100(w/v).
In a preferred embodiment of the present invention, the grain size of pea protein is in the suspension obtained in step (1)
50-70nm, Zeta- current potential are -30--38mV, polydispersity 0.20-0.25, surface tension 60-75mN/m.
In a preferred embodiment of the present invention, homogenizing time is 10-30min in step (1).
In a preferred embodiment of the present invention, it is 500-2,000rpm that centrifugation, which is rate, in step (1).
In a preferred embodiment of the present invention, the best Micro Fluid condition chosen in step (2) is pressure 150-
The weight ratio of 200MPa, cycle-index 2-4 times, 4 DEG C of temperature, pea protein and curcumin is 25:1-60:1.
In the more preferable embodiment of the present invention, the best Micro Fluid condition chosen in step (2) is pressure
200MPa, cycle-index 2 times, 4 DEG C of temperature.
As the third aspect of the present invention, the present invention provides a kind of compounds, including above-mentioned pea protein-curcumin
Nano-complex and water, wherein the amount ratio of the pea protein-curcumin nano compound and water is 5-20:100(w/v).
In a preferred embodiment of the present invention, the pea protein of the compound-curcumin nano compound and water
A concentration of 125-180mg/L of curcumin in the suspension of formation.
The present invention a more preferable embodiment in, pea protein-curcumin nano compound of the compound with
A concentration of 160-180mg/L of curcumin in the suspension that water is formed.
As the fourth aspect of the present invention, the present invention provides above-mentioned pea protein-curcumin nano compounds to prepare
Purposes in antitumor, immunological regulation, anti-oxidant or Antiatherosclerosis medicine.
Compared with prior art, method of the invention has the advantages that：
(1) pea protein-curcumin nano compound of the invention reduces the crystallinity of curcumin, improves curcumin
Thermal stability and dispersibility in water.
(2) mode with tradition dispersion and dissolving curcumin (is that curcumin is dissolved into ethyl alcohol, is then being distributed to
In the carriers such as albumen or polysaccharide, volatilized by alcohol solvent, prepare curcumin or its compound) it compares, pea egg of the invention
In vain-curcumin can be dispersed directly into aqueous solution and have higher dispersion degree.
(3) preparation method of pea protein-curcumin nano compound of the invention is one new in solid field of dispersions
Innovation, change the defect of conventional solid dispersion, it is proposed that it is instantaneously mixed that curcumin is dismantled --- --- by Micro Fluid by one kind
The method that --- --- polymerize again is closed, greatly improves the dispersion efficiency of curcumin and in pea protein-curcumin nano mixed emulsion
In concentration.
(4) preparation method of pea protein-curcumin nano compound of the invention is using pea protein as carrier, breast
Agent and stabilizer, optimization obtains the combination ratio of carrier pea protein and curcumin, under conditions of Micro Fluid, by solid ginger
Flavine is distributed in pea protein carrier.When pea protein prepared by the present invention-curcumin nano compound is dispersed in water without
Any emulsifier and chemical reagent need to be added, you can it obtains stability and dispersibility is preferable, the bigger nanometer of curcumin concentration
Compound, and the reaction condition of the preparation method of the present invention is mild, easy to operate, and can be used for being prepared on a large scale pea egg
In vain-curcumin nano compound the advantages of.
Description of the drawings
Fig. 1 is Micro Fluid condition to curcumin concentration in lotion and pea protein-curcumin nano compound grain size
It influences, wherein it is 35 that figure A, which is in the mass ratio of pea protein and curcumin,:1 (w/w), it is micro- under conditions of cycle-index is 2 times
Influence of the pressure of fluidization treatment to the grain size and curcumin concentration of pea protein in lotion-curcumin nano compound, figure B are
It is 35 in the mass ratio of pea protein and curcumin:1 (w/w), under the conditions of pressure is 200MPa, cycle-index is to pea in lotion
The influence of the grain size and curcumin concentration of albumen-curcumin nano compound.
Fig. 2 is curcumin lotion prepared by distinct methods, wherein a pipes are freshly prepared curcumin lotions, and b pipes are micro-
Pea protein lotion after fluidization treatment, g pipes are that pea protein-curcumin nano for being prepared by Ethanol Method (85%) is compound
Object；C-f pipes are that pea protein-curcumin that embodiment 1, embodiment 2 and embodiment 3 and embodiment 4 are prepared on a large scale is received respectively
Rice milk liquid stores the situation after 90d at 4 DEG C.
Fig. 3 is pea protein-curcumin in the conditioning process for optimize Micro Fluid processing pea protein-curcumin nano lotion
Nano-complex lotion (35:1, w/w) the UV-vis spectrograms under different Micro Fluid processing pressures.Wherein, pea protein
Ultraviolet maximum absorption band is in 284nm.PP indicates the absorption peak of pea protein (pea protein) in figure.
Fig. 4 is all band UV-vis spectrograms of curcumin lotion and different ratios pea protein-curcumin nano lotion.
Fig. 5 is the grain size of pea protein-curcumin nano compound prepared by pea protein, curcumin and the present invention.Its
Middle figure A and figure B is the grain-size graph of pea protein and curcumin respectively；It is embodiment 1, embodiment 2 and embodiment 3 respectively to scheme C-F
The grain size distribution of the pea protein being prepared on a large scale with embodiment 4-curcumin nano compound lotion.
Fig. 6 is the SEM figures of pea protein and the pea protein-curcumin nano compound of the invention prepared.Wherein figure A and
Figure B be respectively Micro Fluid before and after the processing pea protein SEM figure, figure C-F be embodiment 1,3 He of embodiment 2 and embodiment respectively
The SEM for curcumin lotion and pea protein-curcumin nano compound that embodiment 4 is prepared on a large scale schemes.
Fig. 7 is the embodiment of the present invention 1 under the conditions of 90 DEG C, what embodiment 2 and embodiment 3 and embodiment 4 were prepared on a large scale
The thermal stability figure of pea protein-curcumin nano compound.
Fig. 8 is pea protein-turmeric that the embodiment of the present invention 1, embodiment 2 and embodiment 3 and embodiment 4 are prepared on a large scale
Change of size of the plain nano-complex nanometer powder in 30d.
Specific implementation mode
The shortcomings that for curcumin nano compound in the prior art, the present inventor pass through in-depth study, will
Microfluidization applies to the preparation of pea protein-curcumin nano compound, and the compound of preparation reduces the knot of curcumin
Brilliant degree improves the thermal stability and dispersibility in water of curcumin.
The present invention is prepared for pea protein-curcumin nano compound to improve curcumin water-soluble by microfluidization
Dispersibility in liquid, this approach includes the following steps：(1) purifying of pea protein；(2) optimization Micro Fluid handles pea protein-
The condition of curcumin nano lotion includes the proportioning of pressure and cycle-index and pea protein and curcumin；(3) according to optimization
Condition, prepare pea protein-curcumin nano compound.
Pea protein purifies
The purpose of pea protein purifying is to remove the impurity of pea protein.Purification process is：Weigh a certain amount of pea
Legumin is scattered in distilled water or deionized water (for example, the ratio of pea protein and distilled water or deionized water is 3-6:100
(w/v), under agitation, homogeneous 10-30min, then Micro Fluid method processing is carried out, the pea protein lotion after having handled
It is centrifuged under the conditions of 500-2,000rpm, collect suspension and the suspension is lyophilized obtains pea protein powder.
Micro Fluid handles the condition of pea protein-curcumin nano lotion
Since the pressure and cycle-index of Micro Fluid processing are to reducing the grain size of pea protein-curcumin and improving curcumin
Concentration there is large effect, therefore the pressure that handles to Micro Fluid of inventor and cycle-index are optimized.Optimized
Cheng Wei：It is certain in pea protein and curcumin ratio, in the case of cycle-index is fixed, study under conditions of differential pressure,
The change of size of the variation of curcumin concentration and pea protein-curcumin nano compound in lotion, according to turmeric in lotion
Plain concentration reaches maximum, and the grain size of pea protein and curcumin nano compound reaches minimum criterion, chooses most
Good pressure.The pressure finally chosen is 150-200MPa, more preferable 200MPa.Then optimum pressure is constant and pea
In the case that albumen and curcumin ratio are certain, study under the conditions of different cycle-indexes, the change of curcumin concentration in lotion
The variation of the grain size of change and pea protein-curcumin nano compound reaches maximum according to curcumin concentration in lotion, and
And the grain size of pea protein and curcumin nano compound reaches minimum criterion, chooses optimum cycle number.Final choosing
The cycle-index taken is 2-4 times, more preferable 2 times.
In addition, inventor also to Micro Fluid processing temperature, mixing speed and pea protein and curcumin ratio into
Optimization is gone.Curcumin decomposes at a high temperature of Micro Fluid in order to prevent, and inventor is 4 DEG C preferred.Inventor passes through according to research
It is 700-1,000rpm to test the mixing speed for having selected Micro Fluid to handle.Pea protein is used as carrier in the present invention, in order to make list
The loading concentrations of the curcumin of the pea protein of position weight maximize, and inventor has selected pea protein and ginger according to research experience
The ratio of flavine is 60:1-25:1.
Prepare pea protein-curcumin nano compound
Inventor is prepared for pea protein-according to the condition (that is, best Micro Fluid condition) of optimization using Micro Fluid method
Curcumin nano compound, and have studied the physicochemical property of the nano-complex of preparation.
Inventor also demonstrates the feasibility of high-volume pea protein-curcumin nano compound under the optimal conditions.
Detailed process is：(for example, pressure is 200MPa, cycle-index is 2 times) is by pea protein and ginger under the conditions of best Micro Fluid
The amount of flavine amplifies 5-10 times in proportion, is prepared on a large scale pea protein-curcumin nano compound, has studied and be prepared on a large scale
The compound is simultaneously re-dispersed into according to the ratio of 5-20% in distilled water by the physicochemical property of compound, studies it in storage period
The variation of interior grain size and curcumin concentration, it was demonstrated that method of the invention can be used for being prepared on a large scale pea protein-curcumin and receive
Rice compound.
The principle that the present invention prepares pea protein-curcumin nano compound using Micro Fluid method is (1) in high-speed stirring
Under the conditions of mixing, pea protein protein masses in HIGH PRESSURE TREATMENT in short-term dissociate, and wink occurs with curcumin to increase
When the specific surface area that combines；(2) pea protein is by the more hydrophobic bond of exposure, so as to instantaneously be combined with curcumin, improves ginger
The dispersion degree of flavine in aqueous solution.
Pea protein-curcumin nano compound of the present invention can be directly dispersing in aqueous solution, had and appointed without addition
What emulsifier.And the reaction condition of preparation method is mild, simple operation and other advantages.Have compared with previous methods apparent excellent
More property.
Present invention will be further explained below with reference to specific examples.Specific embodiment is to be with technical solution of the present invention
Under the premise of implemented, give detailed embodiment and operating process.It should be understood that these embodiments are merely to illustrate this
It invents rather than limits the scope of the invention.In the following examples, the experimental methods for specific conditions are not specified, usually according to normal
Rule condition carries out.Unless otherwise indicated, ratio and percents by weight.
Pea protein used in the following example is purchased from U.S.'s NAKED nutritional companies；(purity is more than curcumin
99.99%) purchase is in Sigma companies.Miniflow penetrates machine and is purchased from microfluid international corporation of the U.S. (model：M-110P), the present invention is special
(Chamber) internal diameter is 87 μm in Micro Fluid room in profit；SEM is purchased from Jeol Ltd.'s (model:S-3400N)；
Dynamic Laser particle size analyzer is purchased from Worcestershire Malvern instrument company of Britain (model:Nano-ZS90).
Embodiment 1
(1) purifying (removal of impurities) of pea protein：
Weigh a certain amount of pea protein be scattered in distilled water (weight ratio of pea protein and distilled water be 3:100),
Then under agitation, homogeneous 15min, then handle with M-100P Micro Fluids the sample of above-mentioned processing.Pea after having handled
Legumin lotion centrifuges under the conditions of 1,000rpm, collects suspension freeze-drying.The grain size of pea protein nanoemulsions after purification
For 48.45nm, Zeta- current potentials are -35mV, polydispersity 0.22, surface tension 65.15mN/m.
(2) condition of optimization Micro Fluid processing pea protein-curcumin nano lotion：
It is 35 in Micro Fluid cycle-index 2 times and pea protein (step (1) prepares gained) and curcumin ratio:1(w/
W) it under conditions of, chooses different pressure (30,60,80,120,170 and 200MPa) and optimizes.By the figure A in Fig. 1 it is found that
When pressure reaches 120MPa or so, the grain size of pea protein and curcumin nano compound starts to be decreased obviously in lotion, turmeric
The concentration of element starts to obviously increase, and the grain size of pea protein and curcumin nano compound has been when pressure reaches 150MPa or so
Close to minimum value, and curcumin concentration is already close to maximum value, it is thus determined that best Micro Fluid is pressure 150-200MPa.?
When 200MPa, curcumin concentration reaches maximum and pea protein-curcumin nano compound grain size and reaches minimum, respectively
148.37mg/L and 56.34nm, therefore the pressure of most preferably Micro Fluid is 200MPa；In control Micro Fluid pressure 200MPa and pea
Legumin-curcumin ratio is 35:It is excellent to (1,2,3, the 4 and 5 time) progress of cycle-index of Micro Fluid under conditions of 1 (w/w)
Change, by the figure B in Fig. 1 it is found that at 200MPa, when cycle-index is 2-5 times, curcumin concentration and pea protein-in lotion
The variation of the grain size of curcumin nano compound is not too big, therefore secondary from the best Micro Fluid cycle of practical application angle-determining
Number is 2-4 times.Curcumin concentration reaches maximum in lotion when cycle-index is 2 times and pea protein-curcumin nano is compound
The grain size of object reaches minimum, respectively 146.35mg/L and 55.78nm, therefore most preferably cycle-index is 2 times.
(3) it is 25 to prepare pea protein and curcumin ratio:1 pea protein-curcumin nano compound
In best Micro Fluid condition, (pressure 200MPa, cycle-index are 2 times, 4 DEG C of temperature and stir speed (S.S.) exist
Under 700rpm), it is 25 to take weight ratio:1 pea protein (step (1) prepares gained) and curcumin Micro Fluid prepare pea protein-
Curcumin nano compound lotion, and be freeze-dried the lotion and prepare pea protein-curcumin nano compound nanometer powder.
(4) physicochemical property of pea protein-curcumin nano lotion is studied：
It is 25 with the weight ratio of pea protein and curcumin:In the 1 pea protein-curcumin nano compound lotion prepared
The grain size of compound be 55.10nm, Zeta- current potentials be -34.28mV, polydispersity 0.18, surface tension 46.30mN/m,
A concentration of 175.08mg/L of the curcumin in pea protein-curcumin nano compound lotion.The dissolving of curcumin in water
Degree is 0.5mg/L, therefore the dispersion degree in water of curcumin improves 350 times.
(5) feasibility that verification pea protein-curcumin nano compound is prepared on a large scale：
According to 25:1 ratio is by the amount amplification 5-10 of pea protein used in 3.1 (embodiment 1 prepares gained) and curcumin
Again in Micro Fluid (under optimal conditions：Pressure is 200MPa, and cycle-index is 2 times, and temperature is 4 DEG C of stir speed (S.S.)s in 700rpm) under
Pea protein-curcumin nano compound lotion is prepared, is then freeze-dried the lotion and pea protein-turmeric is prepared
Plain nano-complex powder.
(6) feasibility that pea protein-curcumin nano compound that verification is prepared on a large scale is prepared on a large scale：
The grain size of compound is 65.34nm in the pea protein being prepared on a large scale-curcumin nano compound lotion,
Zeta- current potentials are -36.32mV, and polydispersity 0.24, surface tension 43.01mN/m, curcumin is in pea protein-turmeric
A concentration of 181.21mg/L in plain nano-complex lotion suspension.
Embodiment 2
(1) purifying (removal of impurities) of pea protein：
Weigh a certain amount of pea protein be scattered in distilled water (weight ratio of pea protein and distilled water be 4:100),
Then under agitation, homogeneous 20min, then handle with M-100P Micro Fluids the sample of above-mentioned processing.Pea after having handled
Legumin lotion centrifuges under the conditions of 1,200rpm, collects suspension freeze-drying.The grain size of pea protein nanoemulsions after purification
For 58.05nm, Zeta- current potentials are -34.55mV, polydispersity 0.27, surface tension 63.50mN/m.
(2) it is 35 to prepare pea protein and curcumin ratio:1 pea protein-curcumin nano compound：
In best Micro Fluid condition, (pressure 200MPa, cycle-index are 2 times, 4 DEG C of temperature and stir speed (S.S.) exist
Under 800rpm), it is 35 to take weight ratio:Pea is prepared in 1 pea protein (step (1) prepares gained) and curcumin Micro Fluid
It albumen-curcumin nano compound lotion and is freeze-dried the lotion pea protein-curcumin nano compound is prepared
Powder.
(3) prepared by the physicochemical property of research pea protein-curcumin nano lotion：
The grain size of compound is 55.10nm, Zeta- current potentials in the pea protein of gained-curcumin nano compound lotion
For -32.05mV, polydispersity 0.25, surface tension 42.00mN/m, curcumin is compound in pea protein-curcumin nano
A concentration of 173.47mg/L in object lotion.The solubility of curcumin in water is 0.5mg/L, thus curcumin in water
Dispersion degree improves 346 times.
(4) feasibility that verification pea protein-curcumin nano compound is prepared on a large scale：
According to 35:1 ratio by the amount of pea protein used in step (3) (step (1) prepare gained) and curcumin simultaneously
5-10 times of amplification is in Micro Fluid (under optimal conditions：Pressure is 200MPa, and cycle-index is 2 times, and 4 DEG C of stir speed (S.S.)s of temperature exist
Pea protein-curcumin nano lotion is prepared in 800rpm), is then freeze-dried the lotion and pea protein-is prepared
Curcumin nano compound nanometer powder.
(5) feasibility that pea protein-curcumin nano compound that verification is prepared on a large scale is prepared on a large scale：
The grain size of compound is 62.10nm in the pea protein being prepared on a large scale-curcumin nano compound lotion,
Zeta- current potentials are -33.86mV, and polydispersity 0.19, surface tension 42.49mN/m, curcumin is in pea protein-turmeric
A concentration of 179.03mg/L in plain nano-complex lotion suspension.
Embodiment 3
(1) purifying (removal of impurities) of pea protein：
Weigh a certain amount of pea protein be scattered in distilled water (weight ratio of pea protein and distilled water be 5:100),
Then under agitation, homogeneous 25min, then handle with M-100P Micro Fluids the sample of above-mentioned processing.Pea after having handled
Legumin lotion centrifuges under the conditions of 1,000rpm, collects suspension freeze-drying.The grain size of pea protein nanoemulsions after purification
For 52.47nm, Zeta- current potentials are -33.45mV, polydispersity 0.23, surface tension 63.58mN/m.
(2) it is 50 to prepare pea protein and curcumin ratio:1 pea protein-curcumin nano compound：
In best Micro Fluid condition, (pressure 200MPa, cycle-index are 2 times, 4 DEG C of temperature and stir speed (S.S.) are
Under 900rpm), it is 50 to take weight ratio:1 pea protein (step (1) prepares gained) and curcumin Micro Fluid prepares pea egg
In vain-curcumin nano compound lotion, and be freeze-dried the lotion pea protein-curcumin nano compound is prepared and receive
Rice composite powder.
(3) physicochemical property of pea protein-curcumin nano lotion is studied：
It is being 50 with the weight ratio of pea protein-curcumin:1 pea protein-curcumin nano compound the lotion prepared
The grain size of middle compound is 55.10nm, and Zeta- current potentials are -35.20mV, polydispersity 0.21, surface tension 42.00mN/
M, a concentration of 175.08mg/L of the curcumin in pea protein-curcumin nano lotion.The solubility of curcumin in water is
0.5mg/L, therefore the dispersion degree in water of curcumin improves 350 times.
(4) feasibility that verification pea protein-curcumin nano compound is prepared on a large scale：
According to 50:1 ratio by the amount of pea protein used in step (2) (step (1) prepare gained) and curcumin simultaneously
5-10 times of amplification is in Micro Fluid (under optimal conditions：Pressure is 200MPa, and cycle-index is 2 times, stir speed (S.S.) 700rpm) in
Pea protein-curcumin nano compound lotion is prepared, then freeze-drying prepares pea protein-curcumin nano compound and receives
Rice flour end.
(5) physicochemical property for pea protein-curcumin nano lotion that research is prepared on a large scale：
The grain size of compound is 73.21nm in the pea protein being prepared on a large scale-curcumin nano compound lotion,
Zeta- current potentials are -38.24mV, and polydispersity 0.25, surface tension 42.32mN/m, curcumin is in pea protein-turmeric
A concentration of 183.21mg/L in plain nano-complex lotion suspension.
Embodiment 4
(1) purifying (removal of impurities) of pea protein：
Weigh a certain amount of pea protein be scattered in distilled water (weight ratio of pea protein and distilled water be 6:100),
Then under agitation, homogeneous 25min, then handle with M-100P Micro Fluids the sample of above-mentioned processing.Pea after having handled
Legumin lotion centrifuges under the conditions of 1,000rpm, collects suspension freeze-drying.The grain size of pea protein nanoemulsions after purification
For 50.36nm, Zeta- current potentials are -32.56mV, polydispersity 0.25, surface tension 64.25mN/m.
(2) it is 60 to prepare pea protein and curcumin ratio:1 pea protein-curcumin nano compound：
(pressure 200MPa, cycle-index are 2 times, and 4 DEG C of stir speed (S.S.)s of temperature are 1000rpm) with optimal conditions, takes
Weight ratio is 60:It is multiple that 1 pea protein (step (1) prepares gained) and curcumin Micro Fluid prepare pea protein-curcumin nano
It closes object lotion and is freeze-dried the lotion and prepare pea protein-curcumin nano compound.
(3) physicochemical property of pea protein-curcumin nano lotion is studied：
It is 60 with the weight ratio of pea protein and curcumin:In the 1 pea protein-curcumin nano compound lotion prepared
The grain size of nano-complex is 55.10nm, and Zeta- current potentials are -33.88mV, and polydispersity 0.27, surface tension is
44.30mN/m, a concentration of 173.58mg/L of the curcumin in pea protein-curcumin nano lotion.Curcumin in water molten
Xie Du is 0.5mg/L, therefore the dispersion degree in water of curcumin improves 346 times.
(4) feasibility that verification pea protein-curcumin nano compound is prepared on a large scale
According to 60:1 ratio by the amount of pea protein used in step (2) (step (1) prepare gained) and curcumin simultaneously
5-10 times of amplification is in Micro Fluid (under optimal conditions：Pressure is 200MP, cycle-index is 2 times, temperature is 4 DEG C and stir speed (S.S.) is
Pea protein-curcumin nano lotion is prepared in 700rpm), it is compound to prepare pea protein-curcumin nano for then freeze-drying
Object nanometer powder.
(5) physicochemical property for pea protein-curcumin nano lotion that research is prepared on a large scale
Pea protein and curcumin be by weight in the pea protein being prepared on a large scale-curcumin nano compound lotion
60:1, grain size 79.21nm, Zeta- current potential is -36.03mV, polydispersity 0.27, surface tension 42.73mN/m, ginger
A concentration of 168.38mg/L of the flavine in pea protein-curcumin nano compound lotion.
Inventor demonstrates the nano-dispersed performance of high-volume pea protein-curcumin nano composite powder：It will implement
Pea protein-curcumin nano powder prepared by example 1-4, is re-dispersed into distilled water according to the ratio of 5-20%, studies it
Variation within storage period.Pea protein-curcumin nano compound (c-f that embodiment 1-4 as shown in Figure 2 is prepared on a large scale
Pipe) due to grain size it is relatively low (50nm or so), be still uniform suspension after storing 90d at 4 DEG C.And freshly prepared ginger
Flavine lotion (a pipes) is precipitated since curcumin grain size is bigger after preparing quickly；And pass through Ethanol Method (85%)
The pea protein of preparation-curcumin nano compound (g pipes) also has obvious precipitation, this is because ethyl alcohol has damaged pea egg
White secondary structure (hydrophobic core of albumen itself), to form pea protein-curcumin nano compound of obvious sediment.
Inventor has studied pea egg in the conditioning process for optimizing Micro Fluid processing pea protein-curcumin nano lotion
In vain-curcumin nano compound lotion (35:1, w/w) the UV-vis spectrograms under different Micro Fluid processing pressures.Such as Fig. 3 institutes
Show, as can be seen from the figure the ultraviolet maximum absorption band of pea protein is in 284nm.After different pressures Micro Fluid pressure treatment, pea
The absorption peak of legumin-curcumin nano compound shifts and has wider absorption peak, reaches threshold in 165MPa absorption peaks
Value.Illustrate that pea protein is combined with curcumin.
Inventor has studied all band UV- of curcumin lotion and different ratios pea protein-curcumin nano compound
Vis spectrograms.As shown in figure 4, as can be seen from the figure the maximum absorption band of curcumin is in 430nm, and pea protein-curcumin
The maximum absorption band of nanoemulsions is displaced to 424nm.This is because pea protein protein masses in HIGH PRESSURE TREATMENT in short-term are sent out
To increase with curcumin the specific surface area instantaneously combined occurs for raw dissociation, while pea protein is by more hydrophobic of exposure
Key improves the dispersion degree of curcumin in water so as to instantaneously be combined with curcumin.
Inventor compares pea protein and curcumin has studied the particle diameter distribution of pea protein-curcumin nano compound
Figure.As shown in figure 5, as can be seen from the figure the grain size of pea protein is 48.17nm, and the grain size of curcumin is 143.20nm,
Bimodal distribution is presented in its grain size, and the grain size of pea protein-curcumin nano compound is substantially less than the grain size of curcumin, illustrates ginger
The combination of flavine and pea protein.
Inventor compares the SEM figures that pea protein has studied pea protein-curcumin nano compound.As shown in fig. 6, from
That pea protein is smooth (figure A) before it can be seen from the figure that processing, and pea protein structure occurs after Micro Fluid processing
Apparent variation (figure B).From nano-complex (25:1,35:1,50:1 and 60:1, w/w) SEM schemes it is found that curcumin is equal
It is even to be distributed in pea protein carrier.
Inventor compares the thermal stability that curcumin has studied pea protein-curcumin nano compound.As shown in fig. 7,
As can be seen from the figure curcumin lotion is after 90 DEG C of high-temperature process 90min, and only remaining 7% curcumin is not degraded, and pea
Legumin-curcumin nano compound lotion there remains 63% pea protein-turmeric equally after 90 DEG C of high-temperature process 90min
Plain nano-complex is not degraded, shows that pea protein-curcumin nano compound has higher thermal stability.
Inventor has studied the change of size that 30d is placed at 4 DEG C of legumin-curcumin nano compound.Such as Fig. 8 institutes
Show, after pea protein-curcumin nano compound that as can be seen from the figure present invention is prepared on a large scale places 30d at 4 DEG C,
Change of size is little.
The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention.The technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the above embodiments and description only describe this
The principle of invention, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these changes
Change and improvement all fall within the protetion scope of the claimed invention.The claimed scope of the invention by appended claims and its
Equivalent thereof.
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Claims (10)
1. a kind of pea protein-curcumin nano compound, the wherein weight ratio of pea protein and curcumin are 25:1-60:1.
2. pea protein according to claim 1-curcumin nano compound, wherein pea protein-curcumin nano are multiple
The grain size for closing object is 50-75nm, polydispersity 0.13-0.25, surface tension 40-47mN/m.
3. pea protein according to claim 1-curcumin nano compound, wherein the grain size of the pea protein is
50-70nm。
4. the preparation method of a kind of pea protein-curcumin nano compound, wherein this approach includes the following steps：
(1) purifying (removal of impurities) of pea protein：It weighs a certain amount of pea protein to be scattered in distilled water or deionized water, stir
Under the conditions of mixing, homogeneous, then Micro Fluid method processing is carried out, the pea protein lotion after having handled is centrifuged, and is collected and is suspended
Liquid is simultaneously lyophilized the suspension and obtains pea protein powder；
(2) condition of optimization Micro Fluid processing pea protein-curcumin nano lotion：The pea protein obtained using step (1)
Powder and curcumin optimization Micro Fluid method prepare the condition of pea protein-curcumin, choose best Micro Fluid condition；
(3) according to the best Micro Fluid condition in step (2), it is multiple to prepare pea protein-curcumin nano using Micro Fluid method
Object lotion is closed, the lotion is then freeze-dried and prepares pea protein-curcumin nano composite powder.
5. the preparation method of pea protein-curcumin nano compound according to claim 4, wherein pea in step (1)
Albumen is 3-6 with distilled water or the amount ratio of deionized water:100(w/v).
6. the preparation method of pea protein-curcumin nano compound according to claim 4, wherein described in step (2)
The condition of Micro Fluid processing is pressure 150-200MPa, cycle-index 2-4 times, 4 DEG C of temperature, the weight of pea protein and curcumin
Than 25:1-60:1.
7. being chosen in the preparation method of pea protein-curcumin nano compound according to claim 6, wherein step (2)
Best Micro Fluid condition be pressure 150-200MPa, cycle-index 2 times, 4 DEG C of temperature.
8. a kind of compound, it includes claim 1-2 any one of them pea protein-curcumin nano compound or rights
It is required that pea protein-curcumin nano compound and water prepared by the preparation method described in any claims of 4-8, wherein described
The amount ratio of pea protein-curcumin nano compound and water is 5-20:100(w/v).
9. the suspension that compound according to claim 8, wherein pea protein-curcumin nano compound and water are formed
A concentration of 125-180mg/L of middle curcumin.
10. compound described in claim 1-2 any one of them pea protein-curcumin nano compound or claim 9
Purposes in preparing antitumor, immunological regulation, anti-oxidant or Antiatherosclerosis medicine.
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