CN108707640A - Water solubility enzymolysis feather albumen powder and preparation method thereof - Google Patents
Water solubility enzymolysis feather albumen powder and preparation method thereof Download PDFInfo
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Abstract
The present invention relates to a kind of water-soluble enzymolysis feather albumen powders and preparation method thereof, and preparation method includes the following steps：Dry feather is taken, aqueous sodium carbonate is added into dry feather, obtains feather alkaline aqueous solution, boiling processing is carried out to feather alkaline aqueous solution under the conditions of nitrogen protection, obtains the feather after boiling；Take the feather after boiling, mashing is handled and is sieved, keratinase and trypsase are added later, pH is adjusted to 7~9, carries out level-one enzymolysis, level-one zymolyte is obtained after enzymolysis, then the pH of level-one zymolyte is adjusted to 6.0~7.5, neutral proteinase is added, carry out secondary enzymolysis, secondary enzymolysis object is boiled into carry out enzyme deactivation after enzymolysis, obtains secondary enzymolysis object；Level-one digests and secondary enzymolysis carries out under agitation in the process；Take secondary enzymolysis object, it is 45~60% to filter and concentrate the filtrate to water content, is spray-dried to obtain the final product.Product is easy to digest and assimilate and product quality is stablized, and feed or high-quality protein raw material can be used as to use.
Description
Technical field
The invention belongs to protein feed technical fields, and in particular to a kind of water-soluble enzymolysis feather albumen powder and its preparation
Method.
Background technology
Feather meal is made of using various poultry feathers as raw material.Feather meal protein content is high, and amino acid compositing characteristic is rich
It is one of the effective source for solving forage protein source anxiety containing glycine, serine, cystine and isoleucine.But feather meal
Keratin content is high, is not easy to be digested and assimilated by animal body.
Both at home and abroad, has the angle egg that a large amount of scholar's researchs are destroyed by the degradation of the biochemical technologies such as alkaline hydrolysis, enzymolysis in feather meal
In vain, the feeding feather meal that production can be absorbed by animal body.Such as with steam and certain pressure come hydrolyze feather can to increase it
Usability, and handled with strong acid and strong base, but limited success.The catabolite appearance of feather meal is usually aterrimus and tool
Bad smell, the serious quality for affecting downstream product.
In the prior art, Authorization Notice No. is that the patent of invention of CN101613733B discloses the preparation side of feather polypeptide
Animal feather is ground into the feather meal of 100 mesh or more by method, including following steps；By feather meal plus water formed feather with
Water mixed liquid is heated to 80~100 DEG C, keeps the temperature 20~50 minutes and sterilizes；Feather by sterilizing is dropped with water mixed liquid temperature
To 37~40 DEG C, trypsase, heat preservation enzymolysis 2~4 hours is added；By the feather and water mixed liquid after trypsin digestion
45~50 DEG C are heated to, alkali formula protease, heat preservation enzymolysis 4~6 hours is added；By after alkali formula protease hydrolyzed feather with
Water mixed liquid is heated to 55~65 DEG C, and papain, heat preservation enzymolysis 7~9 hours is added；After the completion of by three kinds of enzymolysis
Feather is heated to 80~100 DEG C with water mixed liquid, keeps the temperature 10~40 minutes, enzyme is made to inactivate；It will be obtained after so that enzyme inactivation is handled
To feather filtered by molecular membrane filters with water mixed liquid, obtain feather polypeptide concentrate；Feather polypeptide concentrate is existed
It is spray-dried at 180 DEG C~200 DEG C, obtains feather polypeptide pulvis.Preparation method is more more cumbersome and in high-temperature process plumage
Safeguard measure is not taken to amino acid when hair powder.
Invention content
The object of the present invention is to provide a kind of water-soluble enzymolysis feather albumen powder, which digests feather albumen powder, can
The water solubility and digestibility of feather protein matter are improved, Partial Fish Meal can be replaced to be applied in Feed Manufacturing.
It is a further object to provide a kind of preparation methods of water-soluble enzymolysis feather albumen powder.
In order to achieve the goal above, the technical solution that the present invention takes is：The preparation side of water solubility enzymolysis feather albumen powder
Method includes the following steps：
1) pretreatment：Feather is taken, clean and is cleaned, the sundries such as separation stone, rope, plastic foil obtain dry feather；To
Aqueous sodium carbonate is added in dry feather, obtains feather alkaline aqueous solution, to feather alkaline aqueous solution under the conditions of nitrogen protection
Boiling processing is carried out, the feather after boiling is obtained；
2) it is beaten and digests：Take the feather after boiling in step 1), mashing, which is handled, is simultaneously sieved, be added later keratinase and
PH is adjusted to 7~9 by trypsase, carries out level-one enzymolysis, and level-one hydrolysis temperature is 30~60 DEG C, and level-one enzymolysis time is 4~
12h；Level-one zymolyte is obtained, the pH of level-one zymolyte is then adjusted to 6.0~7.5, neutral proteinase is added, carries out two level
Enzymolysis, secondary enzymolysis temperature are 30~50 DEG C, and the secondary enzymolysis time is 2~6h, after enzymolysis by secondary enzymolysis object boil into
Row enzyme deactivation obtains secondary enzymolysis object；Level-one digests and secondary enzymolysis carries out under agitation in the process；
3) filtering and concentrating：The secondary enzymolysis object in step 2) is taken, it is 45~60% to filter and concentrate the filtrate to water content,
Obtain concentrate egg white liquor；
4) dry：It takes the concentrate egg white liquor in step 3) to be spray-dried and digests feather albumen powder to get water solubility.
Further, the flow of the processing of boiling described in step 1) is：Water vapour is first passed through to add feather alkaline aqueous solution
Then heat keeps the temperature 30~60min, cools the temperature to 40~80 DEG C later to 100~135 DEG C.
Further, the amount of the aqueous sodium carbonate added in feather described in step 1) is to be added per in 100g feathers
The aqueous sodium carbonate of 100~500mL.
Further, the mass percent concentration of the aqueous sodium carbonate is 0.1~0.4%.
Further, the additive amount of keratinase described in step 2) be step 1) in dry feather qualities 0.1~
1.0%, trypsase additive amount is 0.1~1.0% of dry feather qualities in step 1).
Further, the additive amount of neutral proteinase described in step 2) be step 1) in dry feather qualities 0.5~
1.5%.
Further, sieving described in step 2) was 10 mesh sieve.
Further, the inlet air temperature being spray-dried described in step 4) is 150~220 DEG C, and leaving air temp is 60~90
℃。
The water-soluble enzymolysis feather albumen powder prepared using the above-mentioned water-soluble preparation method for digesting feather albumen powder.
Neutral proteinase is obtained by the fermented extraction of bacillus subtilis.
Beneficial effects of the present invention：
The preparation method of the water-soluble enzymolysis feather albumen powder of the present invention, during boiling is handled, using nitrogen to plumage
Amino acid in hair is protected, and amino acid is avoided, because high-temperature oxydation loses biological value, amino to can be improved in digestion process
The utilization rate of acid.Complex enzyme is selected to be digested stage by stage to feather, the protein content and small peptide content in enzymolysis product are higher.
Wink-dry is carried out using spray drying, half fat propylhomoserin can be protected, improve the functionality of feather protein.In addition, the water of the present invention
The preparation method of dissolubility enzymolysis feather albumen powder, technological parameter are easy to control, and production cost is low, realize that waste resource recycles,
Economic benefits.
The water-soluble enzymolysis feather albumen powder protein content and small peptide content (accounting for gross protein ratio) of the present invention is higher,
Vitro Digestibility is 93.5%-98.3%, is easy to digest and assimilate, and product quality is stablized, and feed or high-quality protein can be used as former
Material uses.
Specific implementation mode
The technical scheme in the embodiments of the invention will be clearly and completely described below, it is clear that described implementation
Example is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is common
The every other embodiment that technical staff is obtained, shall fall within the protection scope of the present invention.
Embodiment 1
The preparation method of the water-soluble enzymolysis feather albumen powder of the present embodiment, includes the following steps：
1) pretreatment：Feather is taken, clean and is cleaned, the sundries such as separation stone, rope, plastic foil obtain dry feather；It will
Dry feather is placed in pressure cooker, and nitrogen is filled with after being vacuumized to pressure cooker, and mass percent concentration is added into pressure cooker and is
0.2% aqueous sodium carbonate often adds the aqueous sodium carbonate of 100mL, obtains feather alkaline aqueous solution in the dry feathers of 100g.
Boiling processing is carried out to feather alkaline aqueous solution under the conditions of nitrogen protection.Boiling processing flow be：First lead into pressure cooker
Enter water vapour and feather alkaline aqueous solution is heated to 100 DEG C, then keep the temperature 40min, cools the temperature to 60 DEG C later, obtain boiling
Feather afterwards.
2) it is beaten and digests：The feather after boiling in step 1), mashing is taken to handle and cross 10 mesh sieve, keratin is added later
Enzyme and trypsase, the additive amount of keratinase are 0.1% of dry feather qualities in step 1), and trypsase additive amount is step
1) the 0.5% of dry feather qualities in.PH is adjusted to 7, carries out level-one enzymolysis, level-one hydrolysis temperature is 30 DEG C, when level-one digests
Between be 6h；Level-one zymolyte is obtained, the pH of level-one zymolyte is then adjusted to 7.0, neutral proteinase is added, carries out two level enzyme
Solution, the additive amount of neutral proteinase are 0.5% of dry feather qualities in step 1).Secondary enzymolysis temperature is 40 DEG C, secondary enzymolysis
Time is 3h, and secondary enzymolysis object is boiled carry out enzyme deactivation after enzymolysis, obtains secondary enzymolysis object.Level-one digests and secondary enzymolysis mistake
Cheng Zhongjun is carried out under agitation.
3) filtering and concentrating：The secondary enzymolysis object in step 2) is taken, self-discharging centrifugal machine is selected to carry out secondary enzymolysis object
Filtrate is later 45% through being concentrated in vacuo to water content, obtains concentrate egg white liquor by filter.
4) dry：The concentrate egg white liquor in step 3) is taken, pressure spray dryer is selected to spray concentrate egg white liquor
Mist is dried, and the inlet air temperature of spray drying is 150 DEG C, and leaving air temp is 90 DEG C and digests feather albumen powder to get water solubility.
The water-soluble enzymolysis feather albumen powder of the present embodiment is by the above-mentioned water-soluble preparation method system for digesting feather albumen powder
It is standby and obtain.
Embodiment 2
The preparation method of water-soluble enzymolysis feather albumen powder, includes the following steps described in the present embodiment：
1) pretreatment：Feather is taken, clean and is cleaned, the sundries such as separation stone, rope, plastic foil obtain dry feather；It will
Dry feather is placed in pressure cooker, and nitrogen is filled with after being vacuumized to pressure cooker, and mass percent concentration is added into pressure cooker and is
0.1% aqueous sodium carbonate often adds the aqueous sodium carbonate of 200mL, obtains feather alkaline aqueous solution in the dry feathers of 100g.
Boiling processing is carried out to feather alkaline aqueous solution under the conditions of nitrogen protection.Boiling processing flow be：First lead into pressure cooker
Enter water vapour and feather alkaline aqueous solution is heated to 135 DEG C, then keep the temperature 30min, cools the temperature to 40 DEG C later, obtain boiling
Feather afterwards.
2) it is beaten and digests：The feather after boiling in step 1), mashing is taken to handle and cross 10 mesh sieve, keratin is added later
Enzyme and trypsase, the additive amount of keratinase are 1.0% of dry feather qualities in step 1), and trypsase additive amount is step
1) the 0.1% of dry feather qualities in.PH is adjusted to 9, carries out level-one enzymolysis, level-one hydrolysis temperature is 60 DEG C, when level-one digests
Between be 4h；Level-one zymolyte is obtained, the pH of level-one zymolyte is then adjusted to 7.5, neutral proteinase is added, carries out two level enzyme
Solution, the additive amount of neutral proteinase are 1.5% of dry feather qualities in step 1).Secondary enzymolysis temperature is 30 DEG C, secondary enzymolysis
Time is 2h, and secondary enzymolysis object is boiled carry out enzyme deactivation after enzymolysis, obtains secondary enzymolysis object.Level-one digests and secondary enzymolysis mistake
Cheng Zhongjun is carried out under agitation.
3) filtering and concentrating：The secondary enzymolysis object in step 2) is taken, self-discharging centrifugal machine is selected to carry out secondary enzymolysis object
Filtrate is later 60% through being concentrated in vacuo to water content, obtains concentrate egg white liquor by filter.
4) dry：The concentrate egg white liquor in step 3) is taken, pressure spray dryer is selected to spray concentrate egg white liquor
Mist is dried, and the inlet air temperature of spray drying is 220 DEG C, and leaving air temp is 60 DEG C and digests feather albumen powder to get water solubility.
The water-soluble enzymolysis feather albumen powder of the present embodiment is by the above-mentioned water-soluble preparation method system for digesting feather albumen powder
It is standby and obtain.
Embodiment 3
The preparation method of water-soluble enzymolysis feather albumen powder, includes the following steps described in the present embodiment：
1) pretreatment：Feather is taken, clean and is cleaned, the sundries such as separation stone, rope, plastic foil obtain dry feather；It will
Dry feather is placed in pressure cooker, and nitrogen is filled with after being vacuumized to pressure cooker, and mass percent concentration is added into pressure cooker and is
0.4% aqueous sodium carbonate often adds the aqueous sodium carbonate of 500mL, obtains feather alkaline aqueous solution in the dry feathers of 100g.
Boiling processing is carried out to feather alkaline aqueous solution under the conditions of nitrogen protection.Boiling processing flow be：First lead into pressure cooker
Enter water vapour and feather alkaline aqueous solution is heated to 125 DEG C, then keep the temperature 60min, cools the temperature to 80 DEG C later, obtain boiling
Feather afterwards.
2) it is beaten and digests：The feather after boiling in step 1), mashing is taken to handle and cross 10 mesh sieve, keratin is added later
Enzyme and trypsase, the additive amount of keratinase are 0.8% of dry feather qualities in step 1), and trypsase additive amount is step
1) the 1.0% of dry feather qualities in.PH is adjusted to 8, carries out level-one enzymolysis, level-one hydrolysis temperature is 50 DEG C, when level-one digests
Between be 12h；Level-one zymolyte is obtained, the pH of level-one zymolyte is then adjusted to 6.0, neutral proteinase is added, carries out two level
Enzymolysis, the additive amount of neutral proteinase are 1.0% of dry feather qualities in step 1).Secondary enzymolysis temperature is 50 DEG C, two level enzyme
The solution time is 6h, and secondary enzymolysis object is boiled carry out enzyme deactivation after enzymolysis, obtains secondary enzymolysis object.Level-one digests and secondary enzymolysis
It carries out under agitation in the process.
3) filtering and concentrating：The secondary enzymolysis object in step 2) is taken, self-discharging centrifugal machine is selected to carry out secondary enzymolysis object
Filtrate is later 50% through being concentrated in vacuo to water content, obtains concentrate egg white liquor by filter.
4) dry：The concentrate egg white liquor in step 3) is taken, pressure spray dryer is selected to spray concentrate egg white liquor
Mist is dried, and the inlet air temperature of spray drying is 180 DEG C, and leaving air temp is 70 DEG C and digests feather albumen powder to get water solubility.
The water-soluble enzymolysis feather albumen powder of the present embodiment is by the above-mentioned water-soluble preparation method system for digesting feather albumen powder
It is standby and obtain.
Embodiment 4
The preparation method of water-soluble enzymolysis feather albumen powder, includes the following steps described in the present embodiment：
1) pretreatment：Feather is taken, clean and is cleaned, the sundries such as separation stone, rope, plastic foil obtain dry feather；It will
Dry feather is placed in pressure cooker, and nitrogen is filled with after being vacuumized to pressure cooker, and mass percent concentration is added into pressure cooker and is
0.3% aqueous sodium carbonate often adds the aqueous sodium carbonate of 300mL, obtains feather alkaline aqueous solution in the dry feathers of 100g.
Boiling processing is carried out to feather alkaline aqueous solution under the conditions of nitrogen protection.Boiling processing flow be：First lead into pressure cooker
Enter water vapour and feather alkaline aqueous solution is heated to 110 DEG C, then keep the temperature 50min, cools the temperature to 70 DEG C later, obtain boiling
Feather afterwards.
2) it is beaten and digests：The feather after boiling in step 1), mashing is taken to handle and cross 10 mesh sieve, keratin is added later
Enzyme and trypsase, the additive amount of keratinase are 0.5% of dry feather qualities in step 1), and trypsase additive amount is step
1) the 0.5% of dry feather qualities in.PH is adjusted to 7, carries out level-one enzymolysis, level-one hydrolysis temperature is 40 DEG C, when level-one digests
Between be 8h；Level-one zymolyte is obtained, the pH of level-one zymolyte is then adjusted to 7.0, neutral proteinase is added, carries out two level enzyme
Solution, the additive amount of neutral proteinase are 1.2% of dry feather qualities in step 1).Secondary enzymolysis temperature is 40 DEG C, secondary enzymolysis
Time is 5h, and secondary enzymolysis object is boiled carry out enzyme deactivation after enzymolysis, obtains secondary enzymolysis object.Level-one digests and secondary enzymolysis mistake
Cheng Zhongjun is carried out under agitation.
3) filtering and concentrating：The secondary enzymolysis object in step 2) is taken, self-discharging centrifugal machine is selected to carry out secondary enzymolysis object
Filtrate is later 45% through being concentrated in vacuo to water content, obtains concentrate egg white liquor by filter.
4) dry：The concentrate egg white liquor in step 3) is taken, pressure spray dryer is selected to spray concentrate egg white liquor
Mist is dried, and the inlet air temperature of spray drying is 200 DEG C, and leaving air temp is 80 DEG C and digests feather albumen powder to get water solubility.
The water-soluble enzymolysis feather albumen powder of the present embodiment is by the above-mentioned water-soluble preparation method system for digesting feather albumen powder
It is standby and obtain.
Embodiment 5
The preparation method of water-soluble enzymolysis feather albumen powder, includes the following steps described in the present embodiment：
1) pretreatment：Feather is taken, clean and is cleaned, the sundries such as separation stone, rope, plastic foil obtain dry feather；It will
Dry feather is placed in pressure cooker, and nitrogen is filled with after being vacuumized to pressure cooker, and mass percent concentration is added into pressure cooker and is
0.4% aqueous sodium carbonate often adds the aqueous sodium carbonate of 400mL, obtains feather alkaline aqueous solution in the dry feathers of 100g.
Boiling processing is carried out to feather alkaline aqueous solution under the conditions of nitrogen protection.Boiling processing flow be：First lead into pressure cooker
Enter water vapour and feather alkaline aqueous solution is heated to 120 DEG C, then keep the temperature 40min, cools the temperature to 50 DEG C later, obtain boiling
Feather afterwards.
2) it is beaten and digests：The feather after boiling in step 1), mashing is taken to handle and cross 10 mesh sieve, keratin is added later
Enzyme and trypsase, the additive amount of keratinase are 0.3% of dry feather qualities in step 1), and trypsase additive amount is step
1) the 0.8% of dry feather qualities in.PH is adjusted to 8, carries out level-one enzymolysis, level-one hydrolysis temperature is 30 DEG C, when level-one digests
Between be 10h；Level-one zymolyte is obtained, the pH of level-one zymolyte is then adjusted to 7.5, neutral proteinase is added, carries out two level
Enzymolysis, the additive amount of neutral proteinase are 0.8% of dry feather qualities in step 1).Secondary enzymolysis temperature is 30 DEG C, two level enzyme
The solution time is 4h, and secondary enzymolysis object is boiled carry out enzyme deactivation after enzymolysis, obtains secondary enzymolysis object.Level-one digests and secondary enzymolysis
It carries out under agitation in the process.
3) filtering and concentrating：The secondary enzymolysis object in step 2) is taken, self-discharging centrifugal machine is selected to carry out secondary enzymolysis object
Filtrate is later 60% through being concentrated in vacuo to water content, obtains concentrate egg white liquor by filter.
4) dry：The concentrate egg white liquor in step 3) is taken, pressure spray dryer is selected to spray concentrate egg white liquor
Mist is dried, and the inlet air temperature of spray drying is 150 DEG C, and leaving air temp is 70 DEG C and digests feather albumen powder to get water solubility.
The water-soluble enzymolysis feather albumen powder of the present embodiment is by the above-mentioned water-soluble preparation method system for digesting feather albumen powder
It is standby and obtain.
Test example
Measure the Vitro Digestibility of the water-soluble enzymolysis feather albumen powder prepared by embodiment 1-5, measurement result such as 1 institute of table
Show.The measurement of Vitro Digestibility is with reference to standard GB/T/T 17811-2008 animal protein feed Pepsin digestibilities
Assay method.
Protein content and small peptide content in water-soluble enzymolysis feather albumen powder prepared by embodiment 1-5 are measured, is measured
The results are shown in Table 1.The measurement of protein content and small peptide content is with reference to the assay method in GB/T 22492-2008.
Protein content, small peptide content, the Vitro Digestibility of the water-soluble enzymolysis feather albumen powder of table 1
By table 1 as it can be seen that the protein content of the water-soluble enzymolysis feather albumen powder of the present invention is more than or equal to 80%, small peptide contains
Amount is more than or equal to 85%, and the Vitro Digestibility of Vitro Digestibility 93.5-98.3%, animal are higher, are easy to digest and assimilate.It can be with
It is used as feed or high-quality protein raw material, realizes that waste resource recycles, economic benefits.
Claims (9)
1. the preparation method of water solubility enzymolysis feather albumen powder, which is characterized in that include the following steps：
1) pretreatment：Dry feather is taken, aqueous sodium carbonate is added into dry feather, feather alkaline aqueous solution is obtained, in nitrogen
Boiling processing is carried out to feather alkaline aqueous solution under the conditions of gas shielded, obtains the feather after boiling；
2) it is beaten and digests：It takes the feather after boiling in step 1), mashing to handle and be sieved, keratinase and pancreas egg is added later
PH is adjusted to 7~9 by white enzyme, carries out level-one enzymolysis, and level-one hydrolysis temperature is 30~60 DEG C, and level-one enzymolysis time is 4~12h；
Level-one zymolyte is obtained, the pH of level-one zymolyte is then adjusted to 6.0~7.5, neutral proteinase is added, carries out two level enzyme
Solution, secondary enzymolysis temperature are 30~50 DEG C, and the secondary enzymolysis time is 2~6h, and secondary enzymolysis object is boiled progress after enzymolysis
Enzyme deactivation obtains secondary enzymolysis object；Level-one digests and secondary enzymolysis carries out under agitation in the process；
3) filtering and concentrating：The secondary enzymolysis object in step 2) is taken, it is 45~60% to filter and concentrate the filtrate to water content, is obtained dense
Pix protein liquid；
4) dry：It takes the concentrate egg white liquor in step 3) to be spray-dried and digests feather albumen powder to get water solubility.
2. the preparation method of water-soluble enzymolysis feather albumen powder according to claim 1, which is characterized in that institute in step 1)
Stating the flow that boiling is handled is：First be passed through water vapour and feather alkaline aqueous solution be heated to 100~135 DEG C, then keep the temperature 30~
60min cools the temperature to 40~80 DEG C later.
3. the preparation method of water-soluble enzymolysis feather albumen powder according to claim 1, which is characterized in that institute in step 1)
The amount for stating the aqueous sodium carbonate added in feather is per the aqueous sodium carbonate for adding 100~500mL in 100g feathers.
4. the preparation method of water-soluble enzymolysis feather albumen powder according to claim 1 or 3, which is characterized in that the carbon
The mass percent concentration of acid sodium aqueous solution is 0.1~0.4%.
5. the preparation method of water-soluble enzymolysis feather albumen powder according to claim 1, which is characterized in that institute in step 2)
The additive amount for stating keratinase is 0.1~1.0% of dry feather qualities in step 1), and trypsase additive amount is to be done in step 1)
The 0.1~1.0% of feather qualities.
6. the preparation method of water-soluble enzymolysis feather albumen powder according to claim 1, which is characterized in that institute in step 2)
The additive amount for stating neutral proteinase is 0.5~1.5% of dry feather qualities in step 1).
7. the preparation method of water-soluble enzymolysis feather albumen powder according to claim 1, which is characterized in that institute in step 2)
It was 10 mesh sieve to state sieving.
8. the preparation method of water-soluble enzymolysis feather albumen powder according to claim 1, which is characterized in that institute in step 4)
The inlet air temperature for stating spray drying is 150~220 DEG C, and leaving air temp is 60~90 DEG C.
9. water-soluble enzymolysis feather protein prepared by the preparation method of water-soluble enzymolysis feather albumen powder as described in claim 1
Powder.
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| CN110150502A (en) * | 2019-07-02 | 2019-08-23 | 广东省家禽科学研究所 | A kind of horse ridge goose special feed and preparation method thereof and method for breeding |
| CN112746092A (en) * | 2020-12-31 | 2021-05-04 | 安徽希普生物科技有限公司 | Method for preparing metal chelating peptide from poultry feather |
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