CN108699506A - 分离装置 - Google Patents

分离装置 Download PDF

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Publication number
CN108699506A
CN108699506A CN201780011565.1A CN201780011565A CN108699506A CN 108699506 A CN108699506 A CN 108699506A CN 201780011565 A CN201780011565 A CN 201780011565A CN 108699506 A CN108699506 A CN 108699506A
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CN
China
Prior art keywords
electrodes
electrode
separation device
flow path
cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201780011565.1A
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English (en)
Chinese (zh)
Inventor
胁坂嘉
胁坂嘉一
高野雅代
糸井隆行
圆城寺隆治
户井雅和
西村友美
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Afi Technology Co
Kyoto University NUC
Original Assignee
Afi Technology Co
Kyoto University NUC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Afi Technology Co, Kyoto University NUC filed Critical Afi Technology Co
Publication of CN108699506A publication Critical patent/CN108699506A/zh
Pending legal-status Critical Current

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00—Separating dispersed particles from liquids by electrostatic effect
    • B03C5/005—Dielectrophoresis, i.e. dielectric particles migrating towards the region of highest field strength
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00—Separating dispersed particles from liquids by electrostatic effect
    • B03C5/02—Separators
    • B03C5/022—Non-uniform field separators
    • B03C5/026—Non-uniform field separators using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0652—Sorting or classification of particles or molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0424—Dielectrophoretic forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C2201/00—Details of magnetic or electrostatic separation
    • B03C2201/26—Details of magnetic or electrostatic separation for use in medical or biological applications
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Sustainable Development (AREA)
  • Electrochemistry (AREA)
  • Microelectronics & Electronic Packaging (AREA)
  • Fluid Mechanics (AREA)
  • Physics & Mathematics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Electrostatic Separation (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201780011565.1A 2016-02-16 2017-01-31 分离装置 Pending CN108699506A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2016026673A JP6796932B2 (ja) 2016-02-16 2016-02-16 分離装置
JP2016-026673 2016-02-16
PCT/JP2017/003301 WO2017141685A1 (ja) 2016-02-16 2017-01-31 分離装置

Publications (1)

Publication Number Publication Date
CN108699506A true CN108699506A (zh) 2018-10-23

Family

ID=59625952

Family Applications (1)

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CN201780011565.1A Pending CN108699506A (zh) 2016-02-16 2017-01-31 分离装置

Country Status (5)

Country Link
US (1) US20210205822A1 (enExample)
EP (1) EP3418373B1 (enExample)
JP (1) JP6796932B2 (enExample)
CN (1) CN108699506A (enExample)
WO (1) WO2017141685A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110628568A (zh) * 2019-09-30 2019-12-31 北京化工大学 用于高通量连续流细胞分离的滑轨式介电泳电极结构

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11454583B2 (en) * 2019-12-27 2022-09-27 Imec Vzw Field-array free flow fractionation
US12181448B2 (en) 2019-12-27 2024-12-31 Imec Vzw Method for continuously separating components from a sample
CN113717846B (zh) * 2020-05-26 2023-08-29 中国科学院青岛生物能源与过程研究所 一种基于介电确定性位移的细胞分选芯片、装置及方法
EP3919171A1 (en) * 2020-06-05 2021-12-08 Ecole Polytechnique Fédérale de Lausanne (EPFL) Dielectrophoresis detection device
EP4201526A1 (en) * 2021-12-22 2023-06-28 Imec VZW Microfluidic device for sorting particles

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6280590B1 (en) * 1996-09-06 2001-08-28 Nanogen, Inc. Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis
US6749736B1 (en) * 1998-06-26 2004-06-15 Evotec Technologies Gmbh Electrode arrangement for the dielectrophoretic diversion of particles
US20080283401A1 (en) * 2007-05-18 2008-11-20 Washington, University Of Time-varying flows for microfluidic particle separation

Family Cites Families (7)

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Publication number Priority date Publication date Assignee Title
GB9916850D0 (en) * 1999-07-20 1999-09-22 Univ Wales Bangor Dielectrophoretic apparatus & method
KR100745754B1 (ko) * 2005-12-29 2007-08-02 삼성전자주식회사 금속 기둥 전극 구조를 포함하는 유전 영동을 이용하여입자를 조작하기 위한 장치 및 그를 이용하여 빠른유속으로 유전 영동에 의하여 입자를 조작할 수 있는 방법
WO2012054904A2 (en) * 2010-10-21 2012-04-26 The Regents Of The University Of California Microfluidics with wirelessly powered electronic circuits
JP5617532B2 (ja) * 2010-10-29 2014-11-05 ソニー株式会社 誘電サイトメトリ装置及び誘電サイトメトリによる細胞分取方法
JP5921829B2 (ja) 2011-07-29 2016-05-24 シャープ株式会社 捕集装置、分離方法、および表示方法
EP2739719A2 (en) * 2011-08-02 2014-06-11 Tokyo Electron Limited System and method for tissue construction using an electric field applicator
EP2980558B1 (en) * 2013-03-26 2019-11-06 Sony Corporation Measurement device and measurement method

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6280590B1 (en) * 1996-09-06 2001-08-28 Nanogen, Inc. Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis
US6749736B1 (en) * 1998-06-26 2004-06-15 Evotec Technologies Gmbh Electrode arrangement for the dielectrophoretic diversion of particles
US20080283401A1 (en) * 2007-05-18 2008-11-20 Washington, University Of Time-varying flows for microfluidic particle separation

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
RODRIGO MARTINEZ-DUARTE 等: "A novel approach to dielectrophoresis using carbon electrodes", 《ELECTROPHORESIS》 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110628568A (zh) * 2019-09-30 2019-12-31 北京化工大学 用于高通量连续流细胞分离的滑轨式介电泳电极结构

Also Published As

Publication number Publication date
EP3418373A4 (en) 2019-08-28
WO2017141685A1 (ja) 2017-08-24
JP2017143763A (ja) 2017-08-24
EP3418373B1 (en) 2022-02-23
JP6796932B2 (ja) 2020-12-09
US20210205822A1 (en) 2021-07-08
EP3418373A1 (en) 2018-12-26

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Effective date of abandoning: 20230721