CN108693359A - Zirconium oxide microballon Rh blood grouping reagent cards and preparation method thereof - Google Patents
Zirconium oxide microballon Rh blood grouping reagent cards and preparation method thereof Download PDFInfo
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- CN108693359A CN108693359A CN201710872017.XA CN201710872017A CN108693359A CN 108693359 A CN108693359 A CN 108693359A CN 201710872017 A CN201710872017 A CN 201710872017A CN 108693359 A CN108693359 A CN 108693359A
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/80—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood groups or blood types or red blood cells
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
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Abstract
The present invention provides a kind of zirconium oxide (ZrO2) microballon Rh blood grouping reagent cards and preparation method thereof, the reagent card includes:The zirconium oxide microballon microtrabeculae pipe of the anti-C antibody of monoclonal containing IgM properties, the zirconium oxide microballon microtrabeculae pipe of the anti-c antibody of the monoclonal containing IgM properties, the zirconium oxide microballon microtrabeculae pipe of the anti-E antibody of the monoclonal containing IgM properties, the zirconium oxide microballon microtrabeculae pipe of the anti-e antibody of the monoclonal containing IgM properties, the zirconium oxide microballon microtrabeculae pipe of monoclonal anti-D containing IgM properties and the zirconium oxide microballon microtrabeculae pipe containing zirconium oxide microballon suspension media.Zirconium oxide microballon Rh blood grouping reagent cards high sensitivity of the present invention, specificity be good and stable quality.
Description
Technical field
The present invention relates to medical sciences.More specifically, the present invention relates to a kind of examinations of zirconium oxide microballon Rh blood groupings
Agent card and preparation method thereof.
Background technology
Rh blood groups are the blood group systems found for the first time for 1940.The discovery of Rh blood groups, it is defeated to more scientifically instructing
Blood works and further increases the laboratory diagnosis of neonatal hemolytic disease and safeguards baby's health, there is very important effect.
Rh blood group systems contain 6 kinds of antigens, i.e. C, c, D, d, E, e.All red blood cell antigen containing D is Rh positive, is otherwise the moon
Property.Rh blood groups are mostly generated by blood transfusion or immunity of pregnancy without natural antibody, antibody, have important clinical significance.Resist once being formed
Body such as inputs Rh positive bloods, serious transfusion reaction can occur again.
Domestic not yet realize standardizes reagent for Rh blood groupings at present, and many medical institutions are still without Rh blood groups
Parting inspection.
At abroad, being useful for the grouping reagents card of Rh blood groups, sephadex is mostly used.But sephadex
Can by temperature, salinity, osmotic pressure, pH variation and cause the aperture of particle that can change, the space outside bed can also change, cause from
Heart tomographic results are impacted.Due to being a kind of gelatinous particle, physical strength is poor, with the extension of storage life, can cause
Deformation, non-specific adsorption is high, can influence test result.In addition, price is also more expensive.
Therefore, this field needs the Rh blood grouping reagent cards that development cost is low, stability is good.
Invention content
The purpose of the present invention is to overcome the above shortcomings and to provide a kind of high sensitivity, specific good and stable quality oxygen
Change zirconium microballon Rh blood grouping reagent cards.
In a first aspect, the present invention provides a kind of zirconium oxide microballon Rh blood grouping reagent cards, the reagent card includes:
The microtrabeculae pipe of the zirconium oxide microballon of the anti-C antibody of monoclonal equipped with the property containing IgM;
The microtrabeculae pipe of the zirconium oxide microballon of the anti-c antibody of monoclonal equipped with the property containing IgM;
The microtrabeculae pipe of the zirconium oxide microballon of the anti-E antibody of monoclonal equipped with the property containing IgM;
The microtrabeculae pipe of the zirconium oxide microballon of the anti-e antibody of monoclonal equipped with the property containing IgM;
The microtrabeculae pipe of the zirconium oxide microballon of monoclonal anti-D equipped with the property containing IgM.
Microtrabeculae pipe equipped with the zirconium oxide microballon containing zirconium oxide microballon suspension media.
Above-mentioned zirconium oxide bead particles are 63-125 meters a diameter of.
Potency >=16 of the anti-C antibody of monoclonal of above-mentioned IgM properties;The potency of the anti-c antibody of monoclonal of above-mentioned IgM properties
≥16;Potency >=16 of the anti-E antibody of monoclonal of above-mentioned IgM properties;The potency of the anti-e antibody of monoclonal of above-mentioned IgM properties >=
16;Potency >=64 of the monoclonal anti-D of above-mentioned IgM properties, and and DVIPhenotypic response is feminine gender.
The volume ratio of above-mentioned zirconium oxide microballon and each antibody is 2:1.
Aluminium foil mounting is set at above-mentioned microtrabeculae tube opening.
The above-mentioned zirconium oxide microballon microtrabeculae pipe containing antibody has 1 respectively;Oxidation containing zirconium oxide microballon suspension media
Zirconium microballon microtrabeculae pipe has 1-3 roots.
Second aspect of the present invention provides the preparation method of above-mentioned zirconium oxide microballon Rh blood grouping reagent cards, the method packet
Include following steps:
(a) the zirconium oxide microballon that particle diameter is 63-125 microns is filtered out, is washed 3-5 times, is removed with microballon suspension media
The bead particles of microballon breakage fragment and aggregation are gone, microballon that obtain even particle size and completely spherical in shape is collected;
(b) microballon and each antibody prepared step (a) is respectively according to volume ratio 2:1 ratio mixing, be configured to containing
The microballon of each antibody;
(c) microballon for preparing step (a) is respectively with microballon suspension media according to volume ratio 2:1 ratio mixing, is prepared
At the microballon containing zirconium oxide microballon suspension media;
(d) according to the amount of 22-28 microlitres of every pipe, the microballon that step (b), step (c) are prepared is dispensed into detection card respectively
Microtrabeculae pipe in.
The above method is further comprising the steps of:
(e) with aluminium-foil paper by press mold mode by the sealing suitable for reading of microtrabeculae pipe.
The component of above-mentioned microballon suspension media is:
PH value is 6.6-6.8.
" water " is distilled water, purified water or deionized water, preferably distilled water in microballon suspension media of the present invention.
The beneficial effects of the invention are as follows:
The first aspect of the invention, it is proposed that a kind of standardized zirconium oxide microballon suspension media system, for aoxidizing
The washing and suspension of zirconium microballon, and the stabilization of antibody and zirconium oxide microballon can be maintained for a long time, feature is:
1, the buffer system with very strong buffer capacity is devised, using potassium phosphate, sodium salt and amino acid composition
Buffer system makes the pH value of system maintain 6.6-6.8.With the single citric acid buffer system phase of blood transfusion field generally use
Than having the characteristics that buffer capacity is stronger, being conducive to that whole system is kept to be in desired buffering range, while ensure that entire
The ionic strength of zirconium oxide microballon suspension media;
2, there is significantly more efficient low salt concn system, from its composition as can be seen that the zirconium oxide microballon of the present invention suspends
Medium using amino acid and sodium chloride as additive, hold low ionic strength environment so that micro phosphate is young, keep by auxiliary
Zirconium oxide bead particles are in ball particle and maintain the diameter of zirconium oxide bead particles (63-125 in required range
Micron);
3, the present invention has unique lubricating system, using certain density bovine serum albumin(BSA) so that red blood cell passes through
Suitable lubricating ability is obtained when zirconium oxide microballon gap, the red blood cell of no agglutination is made to have completely by zirconium oxide bead particles
The ability in gap, and the red blood cell being aggregated cannot then pass through;
4, the present invention has superior protective system, selects sodium benzoate as preservative, using polyvinylpyrrolidone
Synergistic effect has better anti-corrosion ability.It is effectively prevent the procreation of bacterium, obtains the storage life of long period, while not only
It avoids promoting to Rh blood groups point the ionic strength of zirconium oxide microballon suspension media system using Sodium azide chemical preservative
The sensitivity and specificity of type card generate harmful effect, also avoid being metabolized during preservation in generation using antibiotics
The harmful effect that mesosome generates the specificity of zirconium oxide microballon Rh blood grouping reagent cards.
On the other hand, in order to ensure the quality of zirconium oxide microballon Rh blood grouping reagent cards of the present invention, in preparation process
It also needs to screen zirconium oxide microballon, that is, first have to select zirconium oxide microballon appropriate, the condition that should generally meet
It is:It is 63-125 microns to select particle diameter, is washed, is suspended to the zirconium oxide microballon raw material that screening obtains, purpose
It is that the washing of zirconium oxide bead particles is allowed to remove damaged zirconium oxide bead particles, the zirconium oxide bead particles of aggregation, internal diameter in 63-
Other impurity components other than super large or extra small zirconium oxide bead particles and zirconium oxide microballon other than 125 microns.
In another aspect, in order to ensure the quality of zirconium oxide microballon Rh blood grouping reagent cards of the present invention, in preparing journey
Also pair antibody starting material mixed with zirconium oxide microballon screens.
In conclusion the zirconium oxide microballon Rh blood groupings reagent card of the present invention why can have it is excellent special
Property, sensitivity and the storage life up to 1 year, are the synergistic effects for being whole system various composition.It is anti-containing 5 kinds of Rh partings
The zirconium oxide microballon microtrabeculae pipe of body, which is arranged on a card, can ensure that a zirconium oxide microballon Rh blood grouping reagent is used only
Card can complete the inspection of all 5 antigens of Rh blood group systems.Buffer system can maintain the pH that reagent card reaction system needs.
Low salt concn system can ensure that zirconium oxide bead particles are fully swollen and zirconium oxide bead particles diameter is required
In range.Lubricating system can ensure lubricating ability appropriate between zirconium oxide bead particles.Preservative can prevent zirconium oxide
Microballon or antibody fail because of bacterial reproduction.Standardized zirconium oxide microballon can ensure between suitable between bead particles
Gap.Standardized antibody can ensure effective detection of antigen.
Rh blood groups are detected with this zirconium oxide microballon Rh blood grouping reagent card, the positive reaction of generation is not less than 1+.One
As under the conditions of 18-25 DEG C preserve the term of validity be not less than 12 months.
In short, the implementation of the present invention provides standardized zirconium oxide microballon Rh blood grouping reagent card products, each hospital
With Blood Transfusion Services can directly from production supplier from zirconium oxide microballon Rh blood grouping reagent cards that obtain standard consistent, be
Precise Identification Rh partings, guarantee safe transfusion, diagnosing and treating neonatal hemolytic disease creates condition as early as possible.
Description of the drawings
Fig. 1 is zirconium oxide microballon microscope figure;
Fig. 2 is the structural schematic diagram of Rh Blood groupings reagent card of the present invention.
1-8. zirconium oxide microballon microtrabeculae pipes;9. detection reagent card;11. zirconium oxide microballon;12. microballon suspension media;13. micro-
Column tube;14. empty calorie piece.
Specific implementation mode
Below by way of the specific embodiment implementation that the present invention will be described in detail and possessed advantageous effect, it is intended to which help is read
Person is better understood from spirit and substance of the present invention, can not constitute any restriction to the practical range of the present invention.All reagents
It is commercially available with instrument.
Embodiment 1:The preparation of zirconium oxide microballon Rh blood grouping reagent cards
Step 1: the preparation of zirconium oxide microballon suspension media
The zirconium oxide microballon suspension media formula is as follows:
The distillation water dissolution of the above reagent, tune pH value are 6.6-6.8.
Step 2: the preparation of zirconium oxide microballon
It is 63-125 microns to select zirconium oxide microballon, particle diameter.The zirconium oxide microballon suspension media prepared with step 1
Washing 3-5 times, removes the zirconium oxide bead particles of zirconium oxide microballon breakage fragment and aggregation, and collection obtains even particle size
And complete applicable zirconium oxide microballon spherical in shape.
Step 3: the selection of antibody
Select the anti-C antibody of monoclonal of IgM properties, potency >=16;
Select the anti-c antibody of monoclonal of IgM properties, potency >=16;
Select the anti-E antibody of monoclonal of IgM properties, potency >=16;
Select the anti-e antibody of monoclonal of IgM properties, potency >=16;
Select the monoclonal anti-D of IgM properties, potency >=64, and and DVIPhenotypic response is feminine gender.
Step 4: the preparation of zirconium oxide microballon
Each antibody that zirconium oxide microballon prepared by step 2 is selected with step 3 is according to volume ratio 2:1 ratio mixing,
It is configured to the anti-c antibody of zirconium oxide microballon, the monoclonal containing IgM properties of the anti-C antibody of the monoclonal containing IgM properties respectively
The anti-e antibody of zirconium oxide microballon, the monoclonal containing IgM properties of the anti-E antibody of zirconium oxide microballon, the monoclonal containing IgM properties
Zirconium oxide microballon and close have IgM properties monoclonal anti-D zirconium oxide microballon;Zirconium oxide microballon prepared by step 2
The zirconium oxide microballon suspension media prepared with step 1 is according to volume ratio 2:1 ratio mixing, is configured to containing zirconium oxide microballon
The zirconium oxide microballon of suspension media.
Step 5: packing
According to 22-28 microlitres of amount of every pipe, each zirconium oxide microballon that step 4 is prepared is added separately to one piece of blank card
In the microtrabeculae pipe of piece.The above-mentioned zirconium oxide microballon microtrabeculae pipe containing antibody has 1 respectively;Contain zirconium oxide microballon suspension media
Zirconium oxide microballon microtrabeculae pipe has 1-3 roots.Detection card can carry out Rh blood groupings to a sample.It is marked for the ease of identification,
Label paper below reagent card.
Step 6: semi-finished product measure
It is required that in the zirconium oxide microballon microtrabeculae pipe containing antibody, have with red blood cell generation >=1+'s of antibody corresponding antigens
Positive reaction, i.e. red blood cell concentrate on zirconium oxide microballon upper surface, and linear type is presented.And with without containing the corresponding antigen of antibody
Red blood cell generates positive reaction, i.e. red blood cell all can reach micro-pipe bottom by zirconium oxide microballon, be deposited on micro-pipe bottom.
And contain only zirconium oxide microballon suspension media without antibody and the zirconium oxide microballon microtrabeculae Guan Zhonghong of zirconium oxide bead mixture is thin
Born of the same parents all can reach micro-pipe bottom by zirconium oxide microballon, be deposited on micro-pipe bottom, and negative reaction is presented.
Step 7: sealing
With aluminium-foil paper by press mold mode by door sealing on microtrabeculae pipe.In 18-25 DEG C of preservation after labelling label.
Step 8: food preservation test
Above-mentioned zirconium oxide microballon Rh blood grouping reagent card cards save 1 year or more, and during preserving herein, the zirconium oxide is micro-
Pearl Rh blood grouping reagent cards have following testing result:
(1) appearance
Zirconium oxide microballon in zirconium oxide microballon Rh blood grouping reagent card cards should all be in flat-white, and microbead layer upper end has
1~2 millimeter of as clear as crystal colourless transparent liquid should not have bubble and foreign matter between zirconium oxide bead particles.
(2) sensitivity
In microtrabeculae zirconium oxide microballon microtrabeculae pipe containing antibody, have with red blood cell generation >=1+'s of antibody corresponding antigens
Positive reaction, i.e. red blood cell concentrate on zirconium oxide microballon upper surface, and linear type is presented.
(3) specific
In microtrabeculae zirconium oxide microballon microtrabeculae pipe containing antibody, has and generate the positive instead with the red blood cell of antibody corresponding antigens
It answers, i.e., red blood cell concentrates on zirconium oxide microballon upper surface, and linear type is presented.It is produced with the red blood cell without containing the corresponding antigen of antibody
Raw negative reaction, i.e. red blood cell all can reach micro-pipe bottom by zirconium oxide microballon, be deposited on micro-pipe bottom.And without anti-
Body contains only in the zirconium oxide microballon microtrabeculae pipe of zirconium oxide microballon suspension media and zirconium oxide bead mixture that red blood cell can be complete
Portion reaches micro-pipe bottom by zirconium oxide microballon, is deposited on micro-pipe bottom, and negative reaction is presented.
Usual sephadex price is more than 30 times of zirconium oxide.In addition, zirconium oxide is white odorless, tasteless crystal, it is insoluble
Yu Shui, hydrochloric acid and dilute sulfuric acid.Chemical property torpescence, and high-melting-point, high resistivity, height analyse and penetrate rate and the property of low thermal coefficient of expansion
Matter is important heat-resisting material, ceramic insulating material.
The characteristics of zirconium oxide microballon is chemical physical stability height, and non-specific adsorption is low, and the microorganisms such as bacterium will not give birth to
Long, filler back-pressure spy is low.
Keep this field special by the foregoing description of the disclosed embodiments for the description to the embodiment of the present invention above
Industry technical staff can realize or use the present invention.Various modifications to these embodiments carry out those skilled in the art
Say be it will be apparent that the general principles defined herein can without departing from the spirit or scope of the present invention,
It realizes in other embodiments.Therefore, the present invention is not intended to be limited to the embodiments shown herein.
Claims (9)
1. a kind of Rh zirconium oxides microballon blood grouping reagent card, which is characterized in that the reagent card includes:
The microtrabeculae pipe of the zirconium oxide microballon of the anti-C antibody of monoclonal equipped with the property containing IgM;
The microtrabeculae pipe of the zirconium oxide microballon of the anti-c antibody of monoclonal equipped with the property containing IgM;
The microtrabeculae pipe of the zirconium oxide microballon of the anti-E antibody of monoclonal equipped with the property containing IgM;
The microtrabeculae pipe of the zirconium oxide microballon of the anti-e antibody of monoclonal equipped with the property containing IgM;
The microtrabeculae pipe of the zirconium oxide microballon of monoclonal anti-D equipped with the property containing IgM;
Microtrabeculae pipe equipped with the zirconium oxide microballon containing zirconium oxide microballon suspension media.
2. zirconium oxide microballon Rh blood grouping reagent cards as described in claim 1, which is characterized in that the zirconium oxide bead particles
It is 63-125 meters a diameter of.
3. zirconium oxide microballon Rh blood grouping reagent cards as claimed in claim 1 or 2, which is characterized in that the list of the IgM properties
Clone potency >=16 of anti-C antibody;Potency >=16 of the anti-c antibody of monoclonal of the IgM properties;The Dan Ke of the IgM properties
Potency >=16 of grand anti-E antibody;Potency >=16 of the anti-e antibody of monoclonal of the IgM properties;The monoclonal of the IgM properties
Potency >=64 of anti-D, and and DVIPhenotypic response is feminine gender.
4. zirconium oxide microballon Rh blood grouping reagent cards as claimed in claim 3, which is characterized in that the zirconium oxide microballon with it is each
The volume ratio of antibody is 2:1.
5. zirconium oxide microballon Rh blood grouping reagent cards as claimed in claim 4, which is characterized in that set at the microtrabeculae tube opening
Aluminium foil mounting.
6. zirconium oxide microballon Rh blood grouping reagent cards as claimed in claim 4, which is characterized in that the oxidation containing antibody
Zirconium microballon microtrabeculae pipe has 1 respectively;Zirconium oxide microballon microtrabeculae pipe containing zirconium oxide microballon suspension media has 1-3 roots.
7. the preparation method of zirconium oxide microballon Rh blood grouping reagent cards as claimed in claim 4, which is characterized in that the method
Including step:
(a) the zirconium oxide microballon that particle diameter is 63-125 microns is filtered out, is washed 3-5 times with microballon suspension media, is removed micro-
Pearl breakage fragment and the bead particles of aggregation collect microballon that obtain even particle size and completely spherical in shape;
(b) microballon and each antibody prepared step (a) is respectively according to volume ratio 2:1 ratio mixing, is configured to containing each anti-
The microballon of body;
(c) microballon and microballon suspension media prepared step (a) is according to volume ratio 2:1 ratio mixing, is configured to containing aerobic
Change the microballon of zirconium microballon suspension media;
(d) according to the amount of 22-28 microlitres of every pipe, the microballon that step (b), step (c) are prepared is dispensed into the micro- of detection card respectively
In column tube.
8. the preparation method of zirconium oxide microballon Rh blood grouping reagent cards as claimed in claim 7, which is characterized in that the method
It is further comprising the steps of:
(e) with aluminium-foil paper by press mold mode by the sealing suitable for reading of microtrabeculae pipe.
9. the preparation method of zirconium oxide microballon Rh blood grouping reagent cards as described in claim 7 or 8, which is characterized in that described
The component of microballon suspension media is:
PH value is 6.6-6.8.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112067827A (en) * | 2020-11-16 | 2020-12-11 | 天津德祥生物技术有限公司 | Antibody diluent and blood type test card comprising same |
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