CN108690874A - A kind of fast digital pcr chip of recurring number continuously adjustable - Google Patents
A kind of fast digital pcr chip of recurring number continuously adjustable Download PDFInfo
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- CN108690874A CN108690874A CN201810549925.XA CN201810549925A CN108690874A CN 108690874 A CN108690874 A CN 108690874A CN 201810549925 A CN201810549925 A CN 201810549925A CN 108690874 A CN108690874 A CN 108690874A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Abstract
A kind of fast digital pcr chip of recurring number continuously adjustable, including mutually nested high-temperature component, low-temperature components and fluoroscopic examination component, 3 components by thickness it is identical upper piece and bottom sheet glass gluing formed;High-temperature component bottom sheet glass top surface is etched with the multiple first U-shaped runners and serpentine flow path, oil storage pool, sample inlet pool, multiple first limit marks and " ten " font runner, a runner in " ten " font runner is connected to sample inlet pool, two runners are connected to oil storage pool, and Article 4 runner is connected to serpentine flow path;The lower surface of sheet glass is fitted with heating plate under high-temperature component;Low-temperature components bottom sheet glass top surface is etched with the multiple second U-shaped runners and one second limit mark, and the lower surface of sheet glass is fitted with heating plate under low-temperature components;Fluoroscopic examination component bottom sheet glass top surface is etched with drop storage chamber, oil extraction pond and runner;The present invention realizes continuously adjusting for the recurring number of PCR, fast, accurately can quantitatively be detected to biological sample.
Description
Technical field
The present invention relates to microflow controlled biochip technical fields, and in particular to a kind of quick number of recurring number continuously adjustable
Word pcr chip.
Background technology
PCR (Polymerase chain reaction, PCR) is seen as molecular biology field center
Sour amplification in vitro and the core technology quantitatively detected, the detection technique of PCR early stages mainly have gel electrophoresis and fluorescent quantitation
PCR detection techniques, until Vogeldteion and Kinzler proposes a kind of new nucleic acid quantitation technique-digital pcr
(Digital PCR, dPCR).The principle of digital pcr is that PCR reaction solutions are dispersed into thousands of a microresponse units,
Each reaction member contains the neccessary composition for carrying out PCR amplification, therefore can to regard small PCR as anti-for these reaction members
System is answered, PCR amplification is then carried out in temperature cycles.After amplification, the reaction member hair containing target molecule to be detected
Go out fluorescence, be denoted as positive signal, the reaction member without containing target molecule to be detected does not have fluorescence, is denoted as negative signal.Statistics
The quantity of positive signal and negative signal can obtain target molecule to be detected by Poisson distribution calculating and accurately copy
Number, to realize the absolute quantitation detection of target molecule.
Gel electrophoresis and Real-Time Fluorescent Quantitative PCR Technique are the common methods of detection of nucleic acids.But detected through gel electrophoresis is deposited
Sensitivity it is low, it is cumbersome, be only capable of the shortcomings of qualitative analysis.Real-time fluorescence quantitative PCR needs root in quantitative objective molecule
According to Ct values draw standard curve, due to the slight change of Ct values can cause detection accuracy reduce, and can only to target molecule into
Row semi-quantitative analysis.Digital pcr not only overcomes the deficiency of detected through gel electrophoresis, but also by directly counting each reaction member
The number of yin and yang attribute signal represent the copy number of target molecule, target molecule can accurately be detected without standard curve,
Realize the absolute quantification analysis of target molecule.Meanwhile digital pcr the homogeneous reaction liquid of large volume is subdivided into it is tens thousand of small
Reaction member, for low abundance pattern detection, each microresponse unit, which is equivalent to, is enriched with low abundance sample, carries significantly
The high sensitivity of detection.
Digital pcr chip technology based on continuous fluid makes stream by the way that high-temperature component and low-temperature components are arranged in the chips
PCR reaction members in road circulate in different temperature components, and the heat to realize denaturation-renaturation-extension is followed
Ring process.Whole process eliminates the reliance on accurate temperature regulating device and controls heating and cooling, and condition is provided for fast PCR.So
And it is the important parameter during PCR that the existing pcr chip technology based on continuous fluid, which has ignored PCR cycle number,.Li et al.
[Anal.Chem.,2016,88,11384-11389]The research middle finger that nucleic acid precision quantifying is detected using drop formula digital pcr
Go out:PCR cycle number directly affects sensitivity and the accuracy of detection of nucleic acids, and low-circulation number, which causes to expand, insufficient keeps detection sensitive
Degree reduces, and high circulation number is because generating a large amount of non-specific amplifications and then influencing detection accuracy, therefore it is ten to optimize PCR cycle number
Divide necessary.For the digital pcr detected based on planar array type end point fluorescence, control PCR cycle number can be carried effectively
The signal-to-noise ratio of high detection reduces the generation of " raindrop " between yin and yang attribute signal, improves sensitivity and the accuracy of detection.It is detecting
When low abundance sample, appropriate increase PCR cycle number is conducive to positive signal and detected from a large amount of negative signals, to improve
The sensitivity of detection;For high abundance sample, the appropriate PCR cycle number that reduces can make negative signal not by a large amount of positive signals
It is fallen into oblivion, to improve the accuracy of detection.
Christopoulos L_N[PSs [Analytica ChimicaActa,2003,494,1–9]To solve to be based on continuous fluid
Pcr chip can not optimize the deficiency of PCR cycle number, it is proposed that the adjustable pcr chip of PCR cycle number, but chip design is only
Four kinds of fixed PCR cycle numbers can be selected, and the span of recurring number is larger, cannot achieve continuously adjusting for PCR cycle number, it is unfavorable
In based on continuous fluid digital pcr experimental design and based on the fluoroscopic examination of planar array type.Therefore, a kind of PCR cycle number is invented
The quick detection that the digital pcr chip that can be adjusted carries out nucleic acid is very necessary.
Invention content
In order to overcome the disadvantages of the above prior art, the object of the present invention is to provide a kind of recurring number continuously adjustables
Fast digital pcr chip, the absolute quantitation that can be carried out to biological sample quickly, accurate, highly sensitive detects.
In order to achieve the above object, the technical solution that the present invention takes is:
A kind of fast digital pcr chip of recurring number continuously adjustable, including high-temperature component 1, low-temperature components 2 and fluorescence inspection
Component 3 is surveyed, the outer circle of low-temperature components 2 is nested among the inner circle of high-temperature component 1, and fluoroscopic examination component 3 is nested in low-temperature components 2
Inner circle among;The high-temperature component 1, low-temperature components 2 and fluoroscopic examination component 3 by thickness it is identical upper piece and lower sheet glass
Fitting is formed.
The 1 bottom sheet glass top surface of high-temperature component is etched with the multiple first U-shaped runners 11 and serpentine flow path 12, storage
Oil sump 13, sample inlet pool 14, multiple first limits identify 15 and " ten " font runner 16, a runner in " ten " font runner 16
It is connected to sample inlet pool 14, two runners are connected to oil storage pool 13, and are connected to the runner of sample inlet pool 14 and the stream that is connected to oil storage pool 13
Road is mutually perpendicular to, and the Article 4 runner of " ten " font runner 16 is connected to serpentine flow path 12;Multiple first U-shaped runners 11 it is mutual it
Between, and can not be connected between serpentine flow path 12;On high-temperature component 1 sheet glass be equipped with respectively with sample inlet pool 13 and oil storage pool 14
The duct of connection;In the lower surface of 1 time sheet glass of high-temperature component, the underface of the first U-shaped runner 11 and serpentine flow path 12 is bonded
There is heating plate of the temperature control range at 80-100 DEG C.
The 2 bottom sheet glass top surface of low-temperature components is etched with the multiple second U-shaped runners 21 and second gag lever post
Know 22, multiple second U-shaped runners 21 can not be also connected between each other, U-shaped in the lower surface of 2 times sheet glass of low-temperature components, second
The underface of runner 21 is fitted with heating plate of the temperature control range at 50-80 DEG C, and sheet glass is corresponding bottom sheet glass on low-temperature components 2
The complete glass of glass.
The 3 bottom sheet glass top surface of fluoroscopic examination component is etched with drop storage chamber 31, oil extraction pond 32 and the 2nd U
The second of first flow 33 and connection drop storage chamber 31 and oil extraction pond 32 that type runner 21 is connected to drop storage chamber 31
Road 34;Sheet glass is equipped with the duct being connected to oil extraction pond 32 on fluoroscopic examination component 3.
PCR cycle number adjust specific method be:In use, 2 relatively-high temperature component 1 of low-temperature components is rotated certain angle
Degree, the output end for making serpentine flow path 12 first is aligned with the second input terminal 23 of any one the second U-shaped runner 21 be connected to after,
The second output terminal 24 of this second U-shaped runner 21 is aligned with the first input end 17 of the first adjacent U-shaped runner 11 and is connected to, and first
First output end 18 of U-shaped runner 11 can be aligned with the second input terminal 23 of second U-shaped runner 21 of another adjacent and be connected to,
By this mode of communicating, the second input terminal 23 and second output terminal 24 of multiple second U-shaped runners 21 are respectively with corresponding multiple
First output end 18 of one U-shaped runner 11 is connected to the alignment of first input end 17, is cooperated, and is formed alternately through high-temperature component
1 and low-temperature components 2 continuous flow path;PCR reaction reagents are often undergone once in continuous flow path from high-temperature component 1 to low-temperature components
2 process regards one cycle as, therefore rotates that different angles can adjust the first U-shaped runner 11 and the second U-shaped runner 21 connects
Logical quantity finally realizes the adjusting of different recurring numbers.
Second limit is identified 22 and is aligned expression Arbitrary cyclic number with arbitrary first limit mark 15.
The fast digital pcr chip uses the coefficient of thermal expansion of glass for 0.5-1.5 × 10-5/K。
The second flow channel 34 of the connection drop storage chamber 31 and oil extraction pond 32 is controlled at 80 × 50 μm or less.
Beneficial effects of the present invention are:By continuously adjusting the recurring number of chip rotation realization PCR, contribute to number
The condition optimizing of PCR reduces signal-to-noise ratio, improves detection sensitivity and accuracy, can flexibly select different concentration of specimens;It will be into
Sample-drop formation-PCR amplification-fluoroscopic examination collection is together on a chip, detection is quick, easy to operate, prevents from polluting.
Description of the drawings
Fig. 1 is the cross-sectional view of the present invention.
Fig. 2 is 1 structural schematic diagram of high-temperature component.
Fig. 3 is 2 structural schematic diagram of low-temperature components.
Fig. 4 is the cross-sectional view of fluoroscopic examination component 3.
Fig. 5 is cross-sectional view of the present invention in arbitrary recurring number.
Specific implementation mode
It elaborates below in conjunction with the accompanying drawings to the present invention.
As shown in Figure 1, a kind of fast digital pcr chip of recurring number continuously adjustable, including high-temperature component 1, low temperature portion
The outer circle of part 2 and fluoroscopic examination component 3, low-temperature components 2 is nested among the inner circle of high-temperature component 1, and fluoroscopic examination component 3 is nested
Among the inner circle of low-temperature components 2;The high-temperature component 1, low-temperature components 2 are identical by thickness with fluoroscopic examination component 3
Upper piece and bottom sheet glass gluing formed.
As shown in Fig. 2, the 1 bottom sheet glass top surface of high-temperature component is etched with the multiple first U-shaped runners 11 and snake
In shape runner 12, oil storage pool 13, sample inlet pool 14, multiple first limit marks 15 and 16, ten " font runner 16 of " ten " font runner
A runner be connected to sample inlet pool 14, two runners are connected to oil storage pool 13, and be connected to sample inlet pool 14 runner and be connected to store up
The runner of oil sump 13 is mutually perpendicular to, and the Article 4 runner of " ten " font runner 16 is connected to serpentine flow path 12;Multiple first U-shaped streams
Road 11 is mutual, and can not be connected between serpentine flow path 12;On high-temperature component 1 sheet glass be equipped with respectively with sample inlet pool 13
The duct being connected to oil storage pool 14;In the lower surface of 1 time sheet glass of high-temperature component, the first U-shaped runner 11 and serpentine flow path 12
Underface is fitted with heating plate of the temperature control range at 80-100 DEG C.
As shown in figure 3, the 2 bottom sheet glass top surface of low-temperature components is etched with the multiple second U-shaped runners 21 and one
Second limit mark 22, multiple second U-shaped runners 21 can not be also connected between each other, in the following table of 2 times sheet glass of low-temperature components
The underface in face, the second U-shaped runner 21 is fitted with heating plate of the temperature control range at 50-80 DEG C, and sheet glass is on low-temperature components 2
The complete glass of corresponding lower sheet glass.
As shown in figure 4, the 3 bottom sheet glass top surface of fluoroscopic examination component is etched with drop storage chamber 31, oil extraction pond
32, the first flow 33 and connection drop storage chamber 31 that are connected to the second U-shaped runner 21 and drop storage chamber 31 and oil extraction pond
32 second flow channel 34;Sheet glass is equipped with the duct being connected to oil extraction pond 32 on fluoroscopic examination component 3.
In the present embodiment, the protrusion above 2 outer round surface of low-temperature components is recessed above 1 internal circular surfaces of high-temperature component
It falls into, to play the role of limited support to low-temperature components 2, the protrusion above 3 outer round surface of fluoroscopic examination component is embedded in low temperature portion
The recess of 2 internal circular surfaces of part makes serpentine flow path 12, multiple first U-shaped streams to play the role of limited support to detection part 3
Road 11, multiple second U-shaped runners 21, first flow 33, second flow channel 34 and drop storage chamber 31 are in same plane.
PCR cycle number adjust specific method be:
Make serpentine flow path 12 first in use, 2 relatively-high temperature component 1 of low-temperature components is turned an angle with reference to Fig. 5
Output end be aligned and be connected to the second input terminal 23 of any one the second U-shaped runner 21 after, the of this second U-shaped runner 21
Two output ends 24 are aligned with the first input end 17 of the adjacent first U-shaped runner 11 and are connected to, the first output of the first U-shaped runner 11
End 18 can be aligned with the second input terminal 23 of second U-shaped runner 21 of another adjacent and be connected to, by this mode of communicating, Duo Ge
The second input terminal 23 and second output terminal 24 of two U-shaped runners 21 are defeated with the first of corresponding multiple first U-shaped runners 11 respectively
The alignment of outlet 18 and first input end 17 be connected to, is cooperated, and formation is continuous alternately through high-temperature component 1 and low-temperature components 2
Runner;PCR reaction reagents, which often undergo once to regard as to the process of low-temperature components 2 from high-temperature component 1 in continuous flow path, once to follow
Ring, therefore rotate different angle and can adjust the quantity that the first U-shaped runner 11 be connected to the second U-shaped runner 21, final realization
The adjusting of different recurring numbers.
The operation principle of the present invention is that:
Sample solution in sample inlet pool 14 is connected to " ten " font to be formed with the oil-phase solution in oil storage pool 13 by runner
Runner 16 generates drop, and drop subsequently enters serpentine flow path 12 and heated, then drop from the output end of serpentine flow path 12 into
Enter in the corresponding second U-shaped runner 21 and carry out low-temperature heat, and then enters from the second output terminal 24 of the second U-shaped runner 21
It is heated at high temperature in first U-shaped runner 11, is entered later again by the first output end 18 of the first U-shaped runner 11 adjacent
Another second U-shaped runner 21 in carry out low-temperature heat;Drop is handed in the runner that high-temperature component 1 is connected to low-temperature components 2
For flowing through, enter drop storage chamber 31 through runner 33 after multiple cycles, extra oil enters oil extraction pond 32 through runner 34.
In the fast digital pcr chip, high-temperature component 1, low-temperature components 2 and fluoroscopic examination component 3 are with one
The coefficient of thermal expansion of adiabatic expansion coefficient, corrosion resistant glass material, glass is 0.5-1.5 × 10-5/ K, using expanding with heat and contract with cold
Principle, after the multiple first U-shaped runners 11 are aligned connection with corresponding multiple second U-shaped runners 21, high-temperature component 1 and low temperature
Component 2 is since heating expands, the inner circle of the internal circular surfaces of high-temperature component 1 and the outer round surface of low-temperature components 2 and low-temperature components 2
The outer round surface of surface and fluoroscopic examination component 3 can mutually squeeze the sealing for contributing to runner.For improve above-mentioned internal circular surfaces and
The leakproofness and lubricity of outer round surface contact, can be applied a little vaseline.
The application method of the fast digital pcr chip of the recurring number continuously adjustable, includes the following steps:
S1, installation:By high-temperature component 1 it is nested low-temperature components 2 nested fluoroscopic examination component 3 makes it be assembled in one again
It rises, in the internal circular surfaces of high-temperature component 1, the inner circle and outer round surface of low-temperature components 2 and the outer round surface of fluoroscopic examination component 3
A small amount of vaseline is smeared in face, increases lubrication and leakproofness;
S2, PCR cycle number are selected:15 alignment of second limit mark 22 and the first limit mark, selectes 30 cycle conducts
This embodiment scheme, it is desirable that 30 the first U-shaped runners 11 and 30 the second U-shaped runners 21 cooperate, alignment connection;
S3, high-temperature component 1 and low-temperature components 2 preheat:It is powered to heating plate and the temperature of control high-temperature component 1 is 95 respectively
DEG C and low-temperature components 2 temperature be 60 DEG C;High-temperature component 1 and low-temperature components 2 are preheated into 20min, make serpentine flow path 12 and the first U
95 DEG C of the holding of type runner 11 is constant, keeps 60 DEG C of the second U-shaped runner 21 holding constant;
S4, sample solution and oil is added:20 μ L reaction solutions and 70 μ L fluorine are added in sample inlet pool 14 and oil storage pool 13 respectively
Carburetion;
S5, drop generate:Negative pressure is loaded to oil extraction pond 32, sample solution and fluorinated oil converge at " ten " font runner 16
It closes, the drop of Water-In-Oil is generated by the effect of shearing force;
S6, PCR amplification:By the drop of the outlet of " ten " font runner 16 outflow first by temperature control 95 in continuous fluid
DEG C serpentine flow path 12 so that archaeal dna polymerase is activated, drop enters low-temperature components 2 of the temperature control at 60 DEG C by the output end of serpentine flow path
The second U-shaped runner 21 make nucleic acid renaturation, later drop by the second U-shaped runner 21 second output terminal 24 enter temperature control at 95 DEG C
The first U-shaped runner 11 of high-temperature component 1 make nucleic acid denaturation, then the first output end 18 through the first U-shaped runner 11 is again
Make nucleic acid renaturation and extension into 2 corresponding second U-shaped runner 21 of low-temperature components, entire nucleic acid denaturation, renaturation and extension
Process can regard a PCR cycle as;Each drop finally enters fluoroscopic examination after 30 PCR cycle numbers through runner 33
In the drop storage chamber 31 of component 3, extra oil enters oil extraction pond 32 by runner 34 in drop storage chamber 31;
S7, fluoroscopic examination and data analysis:All drops can use Systems for optical inspection after entering drop storage chamber 31
Directly detect the number of fluorescence bright spot in droplet array;By the light and shade number of drop in software statistics droplet array, and pass through
Corresponding conversion can be obtained the absolute value of the sample solution to be tested amplifying nucleic acid;
S8, chip cleaning and replacement inspection fluoroscopic examination component 3:After detection, sample inlet pool 14 and oil storage pool 13 according to
Secondary addition scale remover, second alcohol and water continue to load all runners of negative-pressure irrigation to oil extraction pond 32;Wait for that all cleaning solutions flow into oil extraction
Behind pond 32, by the processing of 3 recycled in its entirety of fluoroscopic examination component.
Claims (8)
1. a kind of fast digital pcr chip of recurring number continuously adjustable, including high-temperature component (1), low-temperature components (2) and fluorescence
Detection part (3), it is characterised in that:The outer circle of low-temperature components (2) is nested among the inner circle of high-temperature component (1), fluoroscopic examination
Component (3) is nested among the inner circle of low-temperature components (2);The high-temperature component (1), low-temperature components (2) and fluoroscopic examination component
(3) by thickness it is identical upper piece and bottom sheet glass gluing formed.
2. a kind of fast digital pcr chip of recurring number continuously adjustable according to claim 1, it is characterised in that:Institute
High-temperature component (1) the bottom sheet glass top surface stated is etched with the multiple first U-shaped runners (11) and serpentine flow path (12), oil storage pool
(13), sample inlet pool (14), multiple first, which limit, identifies (15) and " ten " font runner (16), and one in " ten " font runner (16)
Runner is connected to sample inlet pool (14), and two runners are connected to oil storage pool (13), and is connected to the runner of sample inlet pool (14) and is connected to
The runner of oil storage pool (13) is mutually perpendicular to, and the Article 4 runner of " ten " font runner (16) is connected to serpentine flow path (12);It is multiple
First U-shaped runner (11) between each other, and can not be connected between serpentine flow path (12);Sheet glass is set on high-temperature component (1)
There is the duct being connected to respectively with sample inlet pool (13) and oil storage pool (14);The lower surface of sheet glass, the first U under high-temperature component (1)
It is fitted with heating plate of the temperature control range at 80-100 DEG C immediately below type runner (11) and serpentine flow path (12).
3. a kind of fast digital pcr chip of recurring number continuously adjustable according to claim 1, it is characterised in that:Institute
Low-temperature components (2) the bottom sheet glass top surface stated is etched with the multiple second U-shaped runners (21) and one second limit mark (22),
Multiple second U-shaped runners (21) can not also be connected between each other, the lower surface of sheet glass under low-temperature components (2), the second U-shaped stream
Heating plate of the temperature control range at 50-80 DEG C is fitted with immediately below road (21), sheet glass is corresponding bottom sheet on low-temperature components (2)
The complete glass of glass.
4. a kind of fast digital pcr chip of recurring number continuously adjustable according to claim 1, it is characterised in that:Institute
Fluoroscopic examination component (3) the bottom sheet glass top surface stated is etched with drop storage chamber (31), oil extraction pond (32) and the second U-shaped stream
The of first flow 33 that road (21) is connected to drop storage chamber (31) and connection drop storage chamber (31) and oil extraction pond (32)
Two runners (34);Sheet glass is equipped with the duct being connected to oil extraction pond (32) on fluoroscopic examination component (3).
5. a kind of fast digital pcr chip of recurring number continuously adjustable according to claim 1, which is characterized in that PCR
Recurring number adjust specific method be:In use, low-temperature components (2) relatively-high temperature component (1) is turned an angle, make first
The output end of serpentine flow path (12) is aligned with the second input terminal (23) of any one the second U-shaped runner (21) be connected to after, this
First input end (17) company of alignment of the second output terminal (24) of second U-shaped runner (21) and the adjacent first U-shaped runner (11)
Logical, the first output end (18) of the first U-shaped runner (11) can be with the second input of second U-shaped runner of another adjacent (21)
Hold (23) alignment connection, by this mode of communicating, the second input terminal (23) and second output terminal of multiple second U-shaped runners (21)
(24) it is aligned connection with the first output end (18) of corresponding multiple first U-shaped runners (11) and first input end (17) respectively,
It cooperates, forms the continuous flow path alternately through high-temperature component (1) and low-temperature components (2);PCR reaction reagents are in continuous flow path
In often undergo the angle energy once regarded one cycle as from high-temperature component (1) to the process of low-temperature components (2), therefore rotate different
The quantity that enough the first U-shaped runner (11) of adjustment is connected to the second U-shaped runner (21), finally realizes the adjusting of different recurring numbers.
6. a kind of fast digital pcr chip of recurring number continuously adjustable according to claim 3 or 2, it is characterised in that:
Second limit is identified into (22) and is aligned expression Arbitrary cyclic number with arbitrary first limit mark (15).
7. a kind of fast digital pcr chip of recurring number continuously adjustable according to claim 1, it is characterised in that:Institute
The fast digital pcr chip stated uses the coefficient of thermal expansion of glass for 0.5-1.5 × 10-5/K。
8. a kind of fast digital pcr chip of recurring number continuously adjustable according to claim 1, it is characterised in that:Institute
The second flow channel (34) of the connection drop storage chamber (31) and oil extraction pond (32) stated is controlled at 80 × 50 μm or less.
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| CN201810549925.XA CN108690874B (en) | 2018-05-31 | 2018-05-31 | Rapid digital PCR chip with continuously adjustable cycle number |
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| CN201810549925.XA CN108690874B (en) | 2018-05-31 | 2018-05-31 | Rapid digital PCR chip with continuously adjustable cycle number |
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| CN109536384A (en) * | 2018-12-24 | 2019-03-29 | 中国科学院上海微系统与信息技术研究所 | A kind of digital pcr system and its application for the quick absolute quantitation of nucleic acid |
| CN111558402A (en) * | 2020-03-10 | 2020-08-21 | 青岛英赛特生物科技有限公司 | Air pressure driven centrifugal micro-fluidic detection chip |
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| CN108690874B (en) | 2020-09-01 |
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