CN108676840A - A kind of method of quick detection yeast fermenting property - Google Patents
A kind of method of quick detection yeast fermenting property Download PDFInfo
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- CN108676840A CN108676840A CN201810522235.5A CN201810522235A CN108676840A CN 108676840 A CN108676840 A CN 108676840A CN 201810522235 A CN201810522235 A CN 201810522235A CN 108676840 A CN108676840 A CN 108676840A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/06—Quantitative determination
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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Abstract
The invention discloses a kind of methods of quick detection yeast fermenting property, include the following steps：Centrifugation yeast paste is inoculated in wheat juice, is stirred evenly, to obtain yeast wheat juice mixing suspension, and then is fermented under fermentation temperature, obtain zymotic fluid；Zymotic fluid is taken to survey suspended yeast cell number, to track the upgrowth situation of yeast；Test constantly zymotic fluid pol the, until difference≤0.5 ° P of front and back pol twice；When zymotic fluid pol is down to 6.5 7.5 ° of P, fermentation temperature is risen into reduction of diacetyl temperature, the zymotic fluid is taken to survey the content of total biacetyl, until total diacetyl content≤0.250ppm；Finally after the temperature of zymotic fluid be down to 0.5 0.5 DEG C keep 22 24 hours after, take zymotic fluid survey fermentation index.For the present invention, not only step is simple, easily operated, can rapidly detect yeast items fermenting property, accuracy and reproducibility are higher, and testing cost is relatively low.
Description
Technical field
The invention belongs to yeast fermenting property determination techniques fields, and in particular to a kind of quick detection yeast fermenting property
Method.
Background technology
Beer is with barley malt, rice, hops etc. for primary raw material, a kind of made of being brewed through beer yeast fermenting
Containing CO2Low-concentration ethanol beverage, be the alcoholic drink that alcohol content is minimum in drinks.Beer is mild-natured and sweet in flavor, has clearly
Bitter taste, helpful digestion promote cycle, the effect of nourishing the body.Rich in various vitamins, protein, amino acid, carbon in beer
The nutritional ingredients such as hydrate and minerals.The 9th nutraceutical meeting in the world in 1972 recommends beer to be eaten for human nutrition
Product, people also often call beer " liquid bread ".Beer fermentation is one of the important link in beer production, beer ferment
The quality of matrix amount not only influences the consistency of fermentation process, but also has closely with the flavor of beer, taste and bubble holding property etc.
Relationship.Therefore, in beer fermentation process, it is necessary to quickly and accurately be commented the metabolic activity of yeast in fermentation process
Valence, to ensure that beer fermentation is normally carried out.
Yeast fermenting property is evaluated currently, commonly using EBC fermentation tubes in the world.EBC fermentation tubes are by fermentation tube and low
Warm thermostat composition.When karusen after inoculation ferments in pipe, the carbon dioxide that saccharomycete generates can make the karusen in pipe
Liquid generates convection action, to detect the brewing characteristic of yeast strain, meanwhile, the mating low temperature thermostat bath of fermentation tube is for ensureing
Temperature control in fermentation process.
Although this method can effectively assess the fermenting property of yeast, before using fermentation tube, alkali need to be carried out to fermentation tube
It washes, and is carried out disinfection with Peracetic acid, in use, needing to carry out pressure and temperature control to fermentation tube.This method is not only grasped
Make relatively complicated, detection time is longer, and testing cost is higher.
Invention content
In order to overcome above-mentioned technological deficiency, the object of the present invention is to provide a kind of sides of quick detection yeast fermenting property
Method, this method is easy to operate, can rapidly detect yeast items fermenting property, and accuracy and reproducibility are higher, and detects
Cost is relatively low.
To solve the above-mentioned problems, the present invention is achieved by following technical scheme：
A kind of method of quick detection yeast fermenting property, includes the following steps：
S1, centrifugation yeast paste is inoculated in wheat juice, stirs evenly, obtains yeast wheat juice mixing suspension；
S2, yeast wheat juice mixing suspension obtained by step S1 is fermented under fermentation temperature, obtains zymotic fluid；
S3, zymotic fluid obtained by step S2 is taken to survey suspended yeast cell number, to track the upgrowth situation of yeast；
Zymotic fluid pol obtained by S4, test constantly step S2 the, until difference≤0.5 ° P of front and back pol value twice；
When S5, the zymotic fluid pol obtained by the step S4 are down to 6.5-7.5 ° of P, fermentation temperature described in step S2 is risen into reduction
Biacetyl temperature takes the zymotic fluid to survey the content of total biacetyl, until total diacetyl content≤0.250ppm；
S6, after step S5, after the temperature of the zymotic fluid be down to -0.5-0.5 DEG C keep 22-24 hours after, take
The zymotic fluid surveys fermentation index.
Further, vaccination ways are in step S1：Detection cylinder is added in the wheat juice, then the centrifugation yeast paste is connect
Enter the detection cylinder.
Further, the detection cylinder includes：
Socket, for being inoculated with the wheat juice and the centrifugation yeast paste, and the reading zymotic fluid pol value；
First pipe is connected to the socket, for discharging zymotic fluid；The first pipe is equipped with the first valve；
Second pipe is connected to the socket, for being oxygenated and discharging yeast；The second pipe is equipped with the second valve
Door；
Pedestal is set to the socket bottom；
Saccharometer is set in the socket, for measuring the zymotic fluid pol.
Further, the first pipe is located at the middle part of the socket, and the second pipe is apart from the pedestal 1-
3cm。
Further, the preparation method of centrifugation yeast paste described in step S1 is：Yeast cream is protected in 0-4 DEG C of refrigerator
It deposits 22-24 hours, removes supernatant, bottom yeast paste is centrifuged, centrifuging temperature is 3-5 DEG C, centrifugal speed 3800-4200r/
Min, centrifugation time 25-30min obtain the centrifugation yeast paste.
Further, wheat juice described in step S1 is 13 ° of P wheats juice or 18 ° of P wheat juice.
Further, the mass volume ratio g of the centrifugation yeast paste and 13 ° of P wheat juice:ML is 0.0066:1, it is described
The additive amount for centrifuging yeast paste is 11-13g；Or the mass volume ratio g of the centrifugation yeast paste and 18 ° of P wheat juice:ML is
0.01:1, the additive amount of the centrifugation yeast paste is 15-18g.
Further, the suspended yeast cell number in yeast wheat juice mixing suspension described in step S1 is controlled in 13-20 × 106
A/mL.
Further, fermentation temperature described in step S2 is 9-12 DEG C, and reduction of diacetyl temperature described in step S5 is 13-16
℃。
Further, the measurinng frequency of suspended yeast cell number described in step S3 is that primary, step was surveyed every 22-24 hours
The pol measurinng frequency of zymotic fluid described in rapid S4 is to be surveyed once every 22-24 hours.
Compared with prior art, the beneficial effects of the invention are as follows：
(1) centrifugation yeast paste is inoculated with into wheat by the method for a kind of quick detection yeast fermenting property provided by the invention
It is cultivated in juice, to obtain zymotic fluid, by the suspension cell number of measurement zymotic fluid, the upgrowth situation of yeast can be obtained,
By measuring the pol of zymotic fluid, the hypoglycemic speed of zymotic fluid can be obtained, by measuring the content of total biacetyl, can be obtained
Biacetyl reduction rate, and judge whether fermentation terminates, finally measuring the fermentation index of yeast, (total alcohol, total ester, former wheat juice are dense
Degree, alcoholic strength)；Its testing result can be used for Instructing manufacture, the fermenting property of yeast in prediction production, such as hypoglycemic speed, double second
Acyl reduction rate, flavor substance presentation etc..Not only step is easy for the detection method, can rapidly detect yeast items Fermented
Can, accuracy and reproducibility are higher, and testing cost is relatively low.
(2) method of quick detection yeast fermenting property of the present invention, this method is easy to operate, is not only convenient for wheat juice
With centrifugation yeast paste fermentation, and convenient for zymotic fluid extraction and detection.
Description of the drawings
Specific embodiments of the present invention will be described in further detail below in conjunction with the accompanying drawings, wherein：
Fig. 1 is the flow chart of the method for quick detection yeast fermenting property of the present invention；
Fig. 2 is a kind of overall structure diagram of detection cylinder of the present invention.
Description of symbols：
1, socket；2, first pipe；21, the first valve；3, second pipe；31, the second valve；4, pedestal.
Specific implementation mode
Hereinafter, preferred embodiments of the present invention will be described with reference to the accompanying drawings, it should be understood that preferred reality described herein
Apply example only for the purpose of illustrating and explaining the present invention and is not intended to limit the present invention.
Embodiment 1
(1) preparation of yeast paste is centrifuged：
The yeast of selection is that Beer Brewage yeast removes supernatant by yeast cream in being preserved one day in 0 DEG C of refrigerator
Liquid pours into bottom yeast paste in centrifuge tube, and is put in refrigerated centrifuge, and 30min is centrifuged under 4 DEG C, 4000r/min.It scrapes
Except upper layer impurity, the centrifugation yeast paste that need to be detected is obtained.
(2) present embodiment discloses a kind of methods of quick detection yeast fermenting property, as shown in Figure 1, including following step
Suddenly：
S1, the Beer Brewage wheat juice for selecting 13 ° of P put it into detection cylinder, and are inoculated with made from 13g step (1)
Yeast paste is centrifuged, the mass volume ratio g of yeast paste and 13 ° of P wheat juice is centrifuged:ML is 0.0066:1, it stirs evenly, obtains suspension ferment
Mother cell number is 13 × 106The yeast wheat juice mixing suspension of a/mL；
Specifically, as shown in Fig. 2, detection cylinder includes for being inoculated with wheat juice and centrifuging the socket 1 of yeast paste, for discharging hair
The first pipe 2 of zymotic fluid, the second pipe 3 for being oxygenated and discharging yeast, pedestal 4, the pol for measuring zymotic fluid pol
Meter；The capacity for detecting cylinder is 2.5 liters；
Pedestal 4 is set to 1 bottom of socket, and saccharometer is set in socket 1, and first pipe 2 connects with socket 1 with second pipe 3
It is logical；First pipe 2 is equipped with the first valve 21, and second pipe 3 is equipped with the second valve 31；First pipe 2 is located in socket 1
Portion, second pipe 3 and pedestal 4 are at a distance of 1cm.The structure design of the detection cylinder is conducive to wheat juice and centrifuges the fermentation of yeast paste, with
And be conducive to the extraction of zymotic fluid；
More specifically, cylinder is detected before use, need to 75% alcohol rinse be used, and be put in sterile super-clean bench and dry；
S2, by after yeast wheat juice mixing suspension mixing obtained by step S1, the gauze wrapped socket 1 sterilized with 8 layers, then put
Enter in biochemical cultivation case, ferments at 10 DEG C, obtain zymotic fluid；
S3, suspended yeast cell number was surveyed from 2 extraction step S2 gained zymotic fluids of first pipe every 24 hours, to track ferment
Female upgrowth situation；
S4, every 24 hours zymotic fluid pol values obtained by the step S2 read in socket 1 on saccharometer, until front and back two
Difference≤0.5 ° the P of secondary pol；
Sugar concentration measurement is carried out specifically, the glass saccharometer of suitable gradient is put into zymotic fluid, glass saccharometer uses
Before, it need to be wiped with 75% cotton ball soaked in alcohol, and be put in sterile super-clean bench and dry；Preferably, glass saccharometer selects attached temperature glass
Glass saccharometer；
When S5, the zymotic fluid pol obtained by the step S4 are down to 7 ° of P, temperature is risen to 15 DEG C, extracts and ferments from first pipe 2
Liquid surveys the content of total biacetyl, until total diacetyl content≤0.200ppm；
S6, after step S5, after the temperature of zymotic fluid be down to 0 DEG C keep 24 hours after, from first pipe 2 extract send out
Zymotic fluid surveys total alcohol, total ester, original wort concentration, alcoholic strength.
Embodiment 2
(1) preparation of yeast paste is centrifuged：
Yeast paste is centrifuged in the present embodiment, and the preparation method is the same as that of Example 1.
(2) quickly detected disclosed in the present embodiment the method for yeast fermenting property and embodiment 1 difference lies in：
The S1 of the present embodiment, the Beer Brewage wheat juice for selecting 18 ° of P connect yeast paste is centrifuged made from 18g step (1)
Kind enters in 18 ° of P wheat juice, centrifuges the mass volume ratio g of yeast paste and 18 ° of P wheat juice:ML is 0.01:1, it stirs evenly, is suspended
Yeast count is 20 × 106The yeast wheat juice mixing suspension of a/mL；
When S5, the zymotic fluid pol obtained by the step S4 are down to 7 ° of P, temperature is risen to 15 DEG C, extracts and ferments from first pipe 2
Liquid surveys the content of total biacetyl, until total diacetyl content≤0.250ppm；
Remaining step of the present embodiment is same as Example 1.
Reference examples 1：
Yeast fermenting property data tracking method in practical big production：
Using 13 ° of P wheat juice, zymotic fluid is taken in certain Beer Brewage factory fermentation process to carry out blood counting chamber counting and right daily
Zymotic fluid carries out pol detection；Total biacetyl in zymotic fluid, the preliminary examination zymotic fluid of fermentation 8 days are detected according to zymotic fluid hypoglycemic situation
In, detection zymotic fluid fermentation index.
Note：The zymotic fluid of detection in 24 hours is preliminary examination zymotic fluid after total diacetyl content≤0.200ppm in zymotic fluid.
Reference examples 2：
Yeast fermenting property data tracking method in practical big production：
Using 18 ° of P wheat juice, zymotic fluid is taken in certain Beer Brewage factory fermentation process to carry out blood counting chamber counting and right daily
Zymotic fluid carries out pol detection；Total biacetyl in zymotic fluid, the preliminary examination zymotic fluid of fermentation 8 days are detected according to zymotic fluid hypoglycemic situation
In, detection zymotic fluid fermentation index.
Note：The zymotic fluid of detection in 24 hours is preliminary examination zymotic fluid after total diacetyl content≤0.250ppm in zymotic fluid.
Comparative example 1：
Detection method of the EBC fermentation tubes to yeast fermenting property：
Using 13 ° of P wheat juice, inoculation 13g centrifuges yeast paste, centrifuges the mass volume ratio g of yeast paste and 13 ° of P wheat juice:ML is
0.0066:1, it stirs evenly, it is 13 × 10 to obtain suspension cell number6The yeast wheat juice mixing suspension of a/mL；And it puts it into
The detection for fermenting and carrying out yeast fermenting property in EBC fermentation tubes, calculates its detection time.
Comparative example 2：
Detection method of the EBC fermentation tubes to yeast fermenting property：
Using 18 ° of P wheat juice, inoculation 18g centrifuges yeast paste, centrifuges the mass volume ratio g of yeast paste and 18 ° of P wheat juice:ML is
0.01:1, it stirs evenly, it is 20 × 10 to obtain suspension cell number6The yeast wheat juice mixing suspension of a/mL；And put it into EBC
The detection that yeast fermenting property is fermented and carried out in fermentation tube calculates its detection time.
Effete test embodiment 1
Investigate the stability of the method for the quick detection yeast fermenting property of the present invention：
Test method 1：Using the method for the quick detection yeast fermenting property of embodiment 1, it is repeated 5 times detection, as a result such as
Shown in table 1, table 2.
1 embodiment 1 of table detects obtained fermentation index
2 embodiment 1 of table detects obtained suspended yeast cell number
From table 1, table 2 it is found that the result of 5 detection fermentation indexs and suspended yeast cell number is close, fluctuation is very small, card
The reproducibility of the bright detection method is higher, and stability is preferable.
Test method 2：Using the method for the quick detection yeast fermenting property of embodiment 2, it is repeated 5 times detection, as a result such as
Shown in table 3, table 4.
3 embodiment 2 of table detects obtained fermentation index
4 embodiment 2 of table detects obtained suspended yeast cell number
| Detect number | First day | Second day | Third day | 4th day | 5th day |
| 1 | 35.5 | 79.9 | 24.6 | 17.0 | 8.5 |
| 2 | 37.2 | 75.8 | 25.9 | 18.6 | 9.4 |
| 3 | 35.0 | 78.6 | 21.3 | 19.8 | 7.5 |
| 4 | 33.8 | 74.3 | 23.4 | 18.7 | 6.1 |
| 5 | 34.9 | 77.5 | 25.7 | 18.8 | 9.5 |
From table 3, table 4 it is found that the result of 5 detection fermentation indexs and suspended yeast cell number is close, fluctuation is very small, card
The reproducibility of the bright detection method is higher, and stability is preferable.
Effete test embodiment 2
The method of the quick detection yeast fermenting property of the present invention and yeast fermenting property data tracking in practical big production
The testing result of method compares：
Test method 1：
(1) method for using the quick detection yeast fermenting property of embodiment 1, detects fermentation index and the suspension of zymotic fluid
Yeast count, as a result as shown in table 5, table 6；
(2) yeast fermenting property tracking in the practical big production of reference examples 1 is used, the fermentation index of zymotic fluid is detected
And suspended yeast cell number, as a result as shown in table 7, table 8.
5 embodiment 1 of table detects obtained fermentation index
6 embodiment 1 of table detects obtained suspended yeast cell number
| Detect number | First day | Second day | Third day | 4th day | 5th day |
| 1 | 22.5 | 68.7 | 47.1 | 19.6 | 9.4 |
7 reference examples 1 of table detect obtained fermentation index
8 reference examples 1 of table detect obtained suspended yeast cell number
| Detect number | First day | Second day | Third day | 4th day | 5th day |
| 1 | 24.8 | 70.1 | 45.8 | 17.1 | 7.8 |
The fermentation that the method that can be seen that the quick detection yeast fermenting property of embodiment 1 from table 5-8 detects refers to
The result that yeast fermenting property data tracking method detects in mark and the practical big production of yeast suspension cell number and reference examples 1
Very close, fluctuation is very small, it was demonstrated that detection method accuracy of the invention is higher.
Test method 2：
(1) method for using the quick detection yeast fermenting property of embodiment 2, detects fermentation index and the suspension of zymotic fluid
Yeast count, as a result as shown in table 9, table 10；
(2) yeast fermenting property tracking in the practical big production of reference examples 2 is used, the fermentation index of zymotic fluid is detected
And suspended yeast cell number, as a result as shown in table 11, table 12.
9 embodiment 2 of table detects obtained fermentation index
10 embodiment 2 of table detects obtained suspended yeast cell number
| Detect number | First day | Second day | Third day | 4th day | 5th day |
| 1 | 35.9 | 80.3 | 52.8 | 20.4 | 9.8 |
11 reference examples 2 of table detect obtained fermentation index
12 reference examples 2 of table detect obtained suspended yeast cell number
| Detect number | First day | Second day | Third day | 4th day | 5th day |
| 1 | 33.9 | 79.5 | 48.8 | 21.8 | 8.1 |
The fermentation that the method that can be seen that the quick detection yeast fermenting property of embodiment 2 from table 9-12 detects
Yeast fermenting property data tracking method detects in the practical big production of index and yeast suspension cell number and reference examples 2
As a result very close, fluctuation is very small, it was demonstrated that detection method accuracy of the invention is higher.
Effete test embodiment 3
The method of the quick detection yeast fermenting property of the present invention is with EBC fermentation tubes to the detection method of yeast fermenting property
Detection time comparison：
Test method 1：
(1) method for using the quick detection yeast fermenting property of embodiment 1, detection time is 8 days；
(2) use the EBC fermentation tubes of comparative example 1 to the detection method of yeast fermenting property, detection time is 14 days.
It follows that the method detection time of the present invention is shorter.
Test method 2：
(1) method for using the quick detection yeast fermenting property of embodiment 2, detection time is 9 days；
(2) use the EBC fermentation tubes of comparative example 2 to the detection method of yeast fermenting property, detection time is 15 days.
It follows that the method detection time of the present invention is shorter.
The above described is only a preferred embodiment of the present invention, be not intended to limit the present invention in any form, therefore
It is every without departing from technical solution of the present invention content, according to the technical essence of the invention to made by above example it is any modification,
Equivalent variations and modification, in the range of still falling within technical solution of the present invention.
Claims (10)
1. a kind of method of quick detection yeast fermenting property, which is characterized in that include the following steps：
S1, centrifugation yeast paste is inoculated in wheat juice, stirs evenly, obtains yeast wheat juice mixing suspension；
S2, yeast wheat juice mixing suspension obtained by step S1 is fermented under fermentation temperature, obtains zymotic fluid；
S3, zymotic fluid obtained by step S2 is taken to survey suspended yeast cell number, to track the upgrowth situation of yeast；
Zymotic fluid pol obtained by S4, test constantly step S2 the, until difference≤0.5 ° P of front and back pol value twice；
When S5, the zymotic fluid pol obtained by the step S4 are down to 6.5-7.5 ° of P, fermentation temperature described in step S2 is risen into the double second of reduction
Acyl temperature takes the zymotic fluid to survey the content of total biacetyl, until total diacetyl content≤0.250ppm；
S6, after step S5, after the temperature of the zymotic fluid be down to -0.5-0.5 DEG C keep 22-24 hour after, take described in
Zymotic fluid surveys fermentation index.
2. the method for quick detection yeast fermenting property according to claim 1, which is characterized in that inoculation side in step S1
Formula is：Detection cylinder is added in the wheat juice, then the centrifugation yeast paste is accessed into the detection cylinder.
3. the method for quick detection yeast fermenting property according to claim 2, which is characterized in that the detection cylinder packet
It includes：
Socket, for being inoculated with the wheat juice and the centrifugation yeast paste, and the reading zymotic fluid pol value；
First pipe is connected to the socket, for discharging zymotic fluid；The first pipe is equipped with the first valve；
Second pipe is connected to the socket, for being oxygenated and discharging yeast；The second pipe is equipped with the second valve；
Pedestal is set to the socket bottom；
Saccharometer is set in the socket, for measuring the zymotic fluid pol.
4. the method for quick detection yeast fermenting property according to claim 3, it is characterised in that：The first pipe position
In the middle part of the socket, the second pipe is apart from the pedestal 1-3cm.
5. the method for quick detection yeast fermenting property according to claim 1, it is characterised in that：It is centrifuged described in step S1
The preparation method of yeast paste is：By yeast cream in being preserved 22-24 hours in 0-4 DEG C of refrigerator, supernatant is removed, by bottom ferment
Female mud centrifugation, centrifuging temperature are 3-5 DEG C, centrifugal speed 3800-4200r/min, centrifugation time 25-30min and obtain institute
State centrifugation yeast paste.
6. the method for quick detection yeast fermenting property according to claim 1, it is characterised in that：Wheat juice described in step S1
For 13 ° of P wheats juice or 18 ° of P wheat juice.
7. the method for quick detection yeast fermenting property according to claim 6, it is characterised in that：The centrifugation yeast paste
With the mass volume ratio g of 13 ° of P wheat juice:ML is 0.0066:1, the additive amount of the centrifugation yeast paste is 11-13g；Or institute
State the mass volume ratio g of centrifugation yeast paste and 18 ° of P wheat juice:ML is 0.01:1, it is described centrifugation yeast paste additive amount be
15-18g。
8. the method for quick detection yeast fermenting property according to claim 1, it is characterised in that：Yeast described in step S1
Suspended yeast cell number in wheat juice mixing suspension is controlled in 13-20 × 106A/mL.
9. the method for quick detection yeast fermenting property according to claim 1, it is characterised in that：It ferments described in step S2
Temperature is 9-12 DEG C, and reduction of diacetyl temperature described in step S5 is 13-16 DEG C.
10. the method for quick detection yeast fermenting property according to claim 1, it is characterised in that：Described in step S3
The measurinng frequency of suspended yeast cell number is that primary, the pol measurement frequency of zymotic fluid described in step S4 was surveyed every 22-24 hours
Rate is to be surveyed once every 22-24 hours.
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| CN102220196A (en) * | 2011-05-13 | 2011-10-19 | 中国食品发酵工业研究院 | Method for brewing ultrahigh-gravity beer by ultrahigh-gravity Saccharomyces cerevisiae |
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Application publication date: 20181019 |