CN108670485A - Zebra fish fixing device and fixing method thereof - Google Patents
Zebra fish fixing device and fixing method thereof Download PDFInfo
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- CN108670485A CN108670485A CN201810321543.1A CN201810321543A CN108670485A CN 108670485 A CN108670485 A CN 108670485A CN 201810321543 A CN201810321543 A CN 201810321543A CN 108670485 A CN108670485 A CN 108670485A
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D3/00—Appliances for supporting or fettering animals for operative purposes
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Animal Husbandry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention relates to a zebra fish fixing device and a zebra fish fixing method. This zebra fish fixing device, including the body, fixed slot and storage tank have been seted up on the surface of body, fixed slot and storage tank intercommunication, and the fixed slot is used for the health of fixed zebra fish, and the storage tank is used for the head of holding zebra fish, and the inside wall of storage tank and the head interval of zebra fish. In the zebra fish fixing device, the fixing groove is used for fixing the body of the zebra fish, namely the width of the fixing groove is matched with that of the zebra fish, and the two inner side walls of the fixing groove can prevent the body of the zebra fish from moving. The accommodating groove is used for accommodating the head of the zebra fish, and the inner side wall of the accommodating groove is spaced from the head of the zebra fish, so that eye movement of the zebra fish can be prevented from being influenced. Therefore, the zebra fish fixing device can fix the zebra fish well and avoid influencing the eye movement of the zebra fish, so that the vestibular eye movement reflex test of the zebra fish is facilitated, and errors caused by damage to the eye movement muscles due to anesthesia can be eliminated.
Description
Technical field
The present invention relates to vestibular hearing technology fields, more particularly to a kind of zebra fish fixing device and its fixing means.
Background technology
Vestibular dysfunction is high as a kind of incidence, endangers more, the huge disease of resource loss, and it is strong to seriously affect the mankind
Health, one third adult once suffered from this disease, and had the tendency that rising year by year.Vestibular dysfunction not only brings dizziness, balance barrier
Hinder, the Somatic discomforts such as secondary hearing impairment, but also social function can be weakened, cause psychology and phrenoblabia.Ototoxic drug
(ammonia benzyl class antibiotic) is abused and environmental pollution, dizziness crowd rise year by year, and dizziness has become a global health and asks
Topic.Hair cell is auditory system and the common permissive cell of balance system, right due to the complexity in auditory system inner ear labyrinth
In auditory hair cell development and gene regulation research can by the hair cell function of research mode animal balance system come
Carry out, frequently with model animal have mouse and zebra fish.
In vestibular sense of hearing research, zebra fish is a kind of excellent model animal.Although zebra fish has unlike mammal
Cochlea, but possess has the sense of hearing and vestibular function as the utricle and sacculus of otolith structure, can detect opposite by head
Vestibular stimulation caused by the linear acceleration that gravimetric tilt generates.Zebra fish idiosome is transparent, be easy to observe vestibular auditory structures with
Development condition.Sequencing is completed in zebra fish genome, with mankind's similarity 87%, it has now been found that and multiple hearing identical with people/
Gene is balanced, correlation test technology maturation, mutant variety is various, is commonly used to the analysis of deaf gene.To the anti-of ototoxic drug
In answering property, zebra fish sensibility is similar to the mankind, and zebra fish build is small, life cycle is very short, breeding is rapid, makes high pass
Amount analysis is possibly realized.So zebra fish has consequence in vestibular/sense of hearing research and is widely applied, it is necessary to build
Found corresponding measuring system.
Vestibulo-ocular reflection reflection (vestibulo-ocular reflex, VOR) can be generated turns to opposite eye movement with head,
To maintain the stabilization of retina image-forming.As the fundamental mechanism of all eye movements, vestibulo-ocular reflection reflection (VOR) is a kind of simple and quick
Effective hearing impaired detection means.And in vestibulo-ocular reflection reflection measurement system, it is particularly significant how to fix zebra fish, wherein
One fixed form is the culture drop by zebra fish anesthesia on the cover slip, and anesthesia makes zebra fish fix on the cover slip,
But anaesthetic damages the nerve of zebra fish and is easy to cause test result and generates error.
Invention content
Based on this, it is necessary to provide a kind of zebra fish fixing device and its fixing means that can preferably fix zebra fish.
The surface of a kind of zebra fish fixing device, including ontology, the ontology offers fixing groove and storage tank, described
Fixing groove is connected to the storage tank, and the fixing groove is used to fix the body of zebra fish, and the storage tank is for accommodating described
The head of zebra fish, and the head interval of the madial wall of the storage tank and the zebra fish.
In above-mentioned zebra fish fixing device, fixing groove be used for fix zebra fish body namely fixing groove width with
The width of the body of zebra fish is adapted to, and two madial walls of fixing groove can prevent the body of zebra fish from moving.For example, working as zebra
Fish is placed vertically, and head is upward, back outwardly (towards working surface) when, two madial walls of fixing groove can prevent zebra
The body bilateral of fish is mobile and moves up and down.And storage tank is used to house the head of zebra fish, and the madial wall of storage tank with
The head interval of zebra fish, can be to avoid the eye movement of influence zebra fish.Therefore, above-mentioned zebra fish fixing device can preferably be consolidated
Determine zebra fish, and be avoided that the eye movement for influencing zebra fish, consequently facilitating carrying out vestibulo-ocular reflection reflection measurement, Er Qieneng to zebra fish
Eliminate the error brought using anesthesia damage eye movement flesh.
The ontology is transparent body in one of the embodiments,.
In one of the embodiments, the storage tank include the first storage tank and the second storage tank, the fixing groove,
First storage tank and the second storage tank are sequentially communicated, and the width of the fixing groove is less than the width of second storage tank
Degree, on the arragement direction of the fixing groove, first storage tank and second storage tank, the width of first storage tank
Degree gradually increases.
The width of the fixing groove is 0.2~0.4mm, the width of second storage tank in one of the embodiments,
For 0.9~1.1mm.
Further include coverslip in one of the embodiments, the coverslip is used to be covered on the work of the ontology
To make on surface, to close the open side of the storage tank, and forms accommodating cavity, the accommodating cavity is used to keep culture medium, for
The zebra fish breathing.
A kind of fixing means of zebra fish, includes the following steps：
Zebra fish fixing device is provided, the zebra fish fixing device includes ontology, and the ontology includes working surface, institute
It states working surface and offers fixing groove and storage tank, the fixing groove is connected to the storage tank, and the fixing groove is for solid
Determine the body of zebra fish, the storage tank is used to house the head of the zebra fish, and the madial wall of the storage tank with it is described
The head interval of zebra fish；And
The body of the zebra fish is stuck in the fixing groove, and makes the head captured of the zebra fish in the appearance
It sets in slot, the madial wall interval on the head of the zebra fish and the storage tank.
The body of the zebra fish is stuck in the fixing groove in one of the embodiments, and makes the zebra
The head captured of fish includes the following steps in the step in the storage tank：
By the zebra fish and for being positioned over working surface, the zebra fish for the culture medium of zebra fish breathing
In the culture medium；
The zebra fish is stirred using needle so that the body of the zebra fish and head respectively with the fixing groove and institute
Storage tank face is stated, the back of the zebra fish is far from the working surface；And
The culture medium is siphoned away using blotting paper, when siphoning away the culture medium, the body of the zebra fish and head point
It does not take advantage of a situation and slides into the fixing groove and the storage tank.
In one of the embodiments, when the body of the zebra fish is stuck in the fixing groove, the head of the zebra fish
After portion is placed in the storage tank, adhesion is applied to cling the body of the zebra fish in the working surface, avoids institute
State zebra fish movement.
In one of the embodiments, when the body of the zebra fish is stuck in the fixing groove, the head of the zebra fish
After portion is placed in the storage tank, on the working surface, lid sets coverslip, to cover the head of the zebra fish.
The coverslip closes the open side of the storage tank in one of the embodiments, and is formed for keeping training
The accommodating cavity for supporting base instills culture medium so that zebra fish breathes in accommodating cavity.
Description of the drawings
Fig. 1 is the stereoscopic schematic diagram of the zebra fish fixing device of the present embodiment；
Fig. 2 is the schematic top plan view of Fig. 1；
Fig. 3 is the schematic top plan view of zebra fish.
Specific implementation mode
In order to make the foregoing objectives, features and advantages of the present invention clearer and more comprehensible, below in conjunction with the accompanying drawings to the present invention
Specific implementation mode be described in detail.Many details are elaborated in the following description in order to fully understand this hair
It is bright.But the invention can be embodied in many other ways as described herein, those skilled in the art can be not
Similar improvement is done in the case of violating intension of the present invention, therefore the present invention is not limited to the specific embodiments disclosed below.
It should be noted that when element is referred to as " being fixed on " another element, it can be directly on another element
Or there may also be elements placed in the middle.When an element is considered as " connection " another element, it can be directly connected to
To another element or it may be simultaneously present centering elements.
Unless otherwise defined, all of technologies and scientific terms used here by the article and belong to the technical field of the present invention
The normally understood meaning of technical staff is identical.Used term is intended merely to description tool in the description of the invention herein
The purpose of the embodiment of body, it is not intended that in the limitation present invention.Term " and or " used herein includes one or more phases
Any and all combinations of the Listed Items of pass.
As shown in Figure 1, Figure 2 and Figure 3, zebra fish fixing device 10 provided by the embodiment, the zebra fish fixing device 10 are used
In fixed zebra fish 20.
Zebra fish fixing device 10 includes ontology 100, and ontology 100 includes opposite installation surface 102 and working surface
104.Working surface 104 offers fixing groove 110 and storage tank 120, and fixing groove 110 is connected to storage tank 120.Fixing groove
110 body 22 for fixing zebra fish, storage tank 120 are used to house the head 24 of zebra fish, and the madial wall of storage tank 120
The head 24 of 120a and zebra fish is spaced.
In above-mentioned zebra fish fixing device 10, fixing groove 110 is used to fix the body 22 namely fixing groove of zebra fish
110 width is adapted to the width of the body 22 of zebra fish, and two madial wall 110a of fixing groove 110 can prevent zebra fish
Body 22 moves.For example, when zebra fish 20 is placed vertically, and head 24 is upward, back 26 is outwardly (towards working surface 104)
When, two madial wall 110a of fixing groove 110 can prevent the body 22 of zebra fish from moving left and right.And storage tank 120 is for holding
The head 24 of zebra fish is set, and the head 24 of the madial wall 120a of storage tank 120 and zebra fish is spaced, it can be to avoid influence zebra
The eye movement of fish 20.Therefore, above-mentioned zebra fish fixing device 10 can preferably fix zebra fish 20 and influence zebra fish
20 eye movement consequently facilitating carrying out vestibulo-ocular reflection reflection measurement to zebra fish 20, and can be eliminated using anesthesia damage eye movement flesh
The error brought.
Further, in the present embodiment, the end face of ontology 100 is run through in the one end of fixing groove 110 far from storage tank 120, holds
Set the other end that ontology 100 is run through in the one end of slot 120 far from fixing groove 110.In this way, convenient for forming fixing groove 110 and housing
Slot 120 also allows for cleaning fixing groove 110 and storage tank 120.
Further, in the present embodiment, storage tank 120 includes the first storage tank 122 and the second storage tank 124, is fixed
Slot 110, the first storage tank 122 and the second storage tank 124 are sequentially communicated, and the width of fixing groove 110 is less than the second storage tank 124
Width, on the arragement direction of fixing groove 110, the first storage tank 122 and the second storage tank 124, the width of the first storage tank 122
Degree gradually increases.Further, in the present embodiment, width and fixing groove of first storage tank 122 close to one end of fixing groove 110
110 width is equal, width of first storage tank 122 close to the width and the second storage tank 124 of one end of the second storage tank 124
It is equal.
Further, in the present embodiment, it is located at the madial wall 110a and the second storage tank 124 of the fixing groove 110 of the same side
Madial wall 124a it is parallel, connect the madial wall 124a of the madial wall 110a and the second storage tank 124 of the fixing groove 110 of the same side
The first storage tank 122 madial wall 122a be tapered plane.
Further, in the present embodiment, the madial wall 122a of the madial wall 110a of fixing groove 110 and the first storage tank 122
Junction formed rounded corner (not shown), in this way, can to avoid wedge angle injure zebra fish 20.
In the present embodiment, zebra fish 20 is zebra juvenile fish.Specifically, zebra fish 20 is the zebra children being born 4~10 days
Fish, at this point, the length of zebra fish 20 is 3~4mm, the width on the head 24 of zebra fish is 0.8~1.0mm.Correspondingly, in this reality
It applies in example, the width of fixing groove 110 is 0.2~0.4mm, and the width of the second storage tank 124 is 0.9~1.1mm.
Further, in the present embodiment, ontology 100 is in cuboid, and the length of ontology 100 is 6.5mm, width 5mm,
Height is 4mm.The size of ontology 100 is smaller, easy to carry.Wherein, the 110, first storage tank of long side Yu fixing groove of ontology 100
122 and second storage tank 124 arragement direction it is parallel, between a height of installation surface 102 and working surface 104 of ontology 100
Distance.
Further, in the present embodiment, ontology 100 is transparent body.In this way, can be by the installation surface 102 of ontology 100
On the light shield of infrared light supply, and zebra fish 20 is located on vertical direction, and the head 24 of zebra fish 20 is relative to spot
The body 22 of horse fish 20 is located above, then thermal camera is arranged in the side where the working surface 104 in ontology 100, black
Under dark situation, the head image for the zebra fish irradiated through infrared light supply is obtained by thermal camera, and zebra is obtained according to image
The vestibulo-ocular reflection reflection results of fish 20.In the present embodiment, infrared light supply, ontology 100 and infrared camera are arranged successively, and are located at
On same straight line, image acquisition mode is transmission-type, and in other embodiments, ontology 100 is arranged with infrared camera face, infrared
Light source be set to infrared camera on, and with 100 face of ontology, image acquisition mode be it is reflective, ontology 100 can be impermeable at this time
Light.
Further, in the present embodiment, ontology 100 is organic glass.
Further, in the present embodiment, zebra fish fixing device 10 further includes coverslip (not shown), and coverslip is for covering
On the working surface 104 of ontology 100, to close the open side of storage tank 120, and accommodating cavity (not shown) is formed, housed
Chamber is for housing the head 24 of zebra fish and for keeping culture medium, so that zebra fish 20 breathes.And positioned at zebra fish
Coverslip at head 24, moreover it is possible to eliminate the pattern distortion that water droplet lens effect is brought.
In the present embodiment, a kind of fixing means of zebra fish is also provided, is included the following steps：
Step S210 provides above-mentioned zebra fish fixing device.
The body of zebra fish is stuck in fixing groove by step S220, and makes the head captured of zebra fish in storage tank,
The head of zebra fish and the madial wall interval of storage tank.
In the present embodiment, step S220 includes the following steps：
Step S222, by zebra fish and for being positioned over working surface, zebra fish position for the culture medium that zebra fish breathes
In culture medium.In the present embodiment, drawn using suction pipe and be incubated at zebra fish and culture medium in culture medium, by zebra fish and
Culture medium drop is on working surface namely zebra fish and culture medium are placed on the work surface simultaneously.It is appreciated that at other
In embodiment, the placement that zebra fish and culture medium can also be one in front and one in back is on the work surface.Specifically, in the present embodiment,
Suction pipe is Bath suction pipe.
Step S224 stirs zebra fish using needle so that the body of zebra fish and head respectively with fixing groove and accommodating
Slot face, the back of zebra fish is far from installation surface.Namely the body of zebra fish and head is made to be located at fixing groove and appearance
Set the top of slot.Specifically, in the present embodiment, zebra fish is stirred using thin copper needle.Zebra fish is stirred using needle, can be made
The body of zebra fish fast and accurately alignment and fixation slot.
Step S226, siphons away culture medium, so that the body of zebra fish is stuck in fixing groove, and makes the head of zebra fish
Portion is placed in storage tank.In the present embodiment, culture medium is siphoned away using blotting paper, when siphoning away culture medium, the body of zebra fish
Body and head are taken advantage of a situation respectively to be slid into fixing groove and storage tank.Specifically, in the present embodiment, blotting paper is institute in daily life
Toilet paper.
It is appreciated that in other embodiments, by zebra fish and culture medium drop after fixing groove, zebra fish master can also be waited
It is dynamic to enter fixing groove.
Step S230 applies adhesion to cling the body of zebra fish in working surface, zebra fish is avoided to move.Specifically
Ground is stained with the brush of adhesion in the present embodiment, and the intersection of self-retaining slot and storage tank is slided along fixing groove, with
Working surface applies adhesion.Namely in the present embodiment, adhesion is applied above fixing groove, is not applied above storage tank
Adhesion.Adhesion can cling the body of zebra fish, and avoiding zebra fish from moving up and down, (head 24 of zebra fish 20 is divided with body 22
Dui Ying not be up and down) so that zebra fish is more firmly fixed on ontology.It is appreciated that in other embodiments, step S230
It can be omitted.
More specifically, in the present embodiment, adhesion is the methocel solution that mass fraction is 3%.In other realities
It applies in example, adhesion may be agar.
Step S240, on the work surface, lid set coverslip, to cover the head of zebra fish.
In the present embodiment, coverslip is adsorbed in by adhesion on working surface.It is appreciated that in other embodiment
In, when step S230 is omitted, clear water can be applied on the work surface, coverslip is adsorbed in by clear water on working surface.
Coverslip is set, the pattern distortion that water droplet lens effect is brought can be eliminated.
Specifically, in the present embodiment, the size of coverslip is consistent with the size of working surface 104, and work is completely covered
Surface 104.More specifically, in the present embodiment, the length of coverslip is 6.5mm, width 5mm.
Step S250, coverslip close the open side of storage tank, and form accommodating cavity, instilled in accommodating cavity culture medium with
It is breathed for zebra fish.Coverslip can not only eliminate the pattern distortion that water droplet lens effect is brought, moreover it can be used to close storage tank
Open side so that culture medium is stable in accommodating cavity.
After fixing zebra fish using the fixing means of above-mentioned zebra fish, the installation surface of ontology is set to the light of infrared light supply
On cover, to carry out obtaining the vestibulo-ocular reflection reflection results of zebra fish, coverslip is finally removed, and zebra fish is rinsed from ontology
Get off, to wait for next use.
The fixing means of above-mentioned zebra fish can be eliminated in vestibulo-ocular reflection reflection measurement, because using anesthesia damage eye movement flesh band
The error come, and the fixing means of above-mentioned zebra fish and vestibulo-ocular reflection reflection measurement are relatively independent, can provide multiple zebras
Fish fixing device fixes zebra fish so as to multi agent cooperation, accelerates testing efficiency, and every fish average cost 2 minutes is conducive to height
Flux is tested.
Each technical characteristic of embodiment described above can be combined arbitrarily, to keep description succinct, not to above-mentioned reality
It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, it is all considered to be the range of this specification record.
Several embodiments of the invention above described embodiment only expresses, the description thereof is more specific and detailed, but simultaneously
It cannot therefore be construed as limiting the scope of the patent.It should be pointed out that coming for those of ordinary skill in the art
It says, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the protection of the present invention
Range.Therefore, the protection domain of patent of the present invention should be determined by the appended claims.
Claims (10)
1. a kind of zebra fish fixing device, which is characterized in that including ontology, the surface of the ontology offers fixing groove and appearance
Slot is set, the fixing groove is connected to the storage tank, and the fixing groove is used to fix the body of zebra fish, and the storage tank is used for
House the head of the zebra fish, and the head interval of the madial wall of the storage tank and the zebra fish.
2. zebra fish fixing device according to claim 1, which is characterized in that the ontology is transparent body.
3. zebra fish fixing device according to claim 1, which is characterized in that the storage tank include the first storage tank with
And second storage tank, the fixing groove, first storage tank and the second storage tank are sequentially communicated, and the width of the fixing groove
Less than the width of second storage tank, in the fixing groove, the arrangement side of first storage tank and second storage tank
Upwards, the width of first storage tank gradually increases.
4. zebra fish fixing device according to claim 3, which is characterized in that the width of the fixing groove be 0.2~
The width of 0.4mm, second storage tank are 0.9~1.1mm.
5. zebra fish fixing device according to claim 1, which is characterized in that further include coverslip, the coverslip is used
In being covered on the working surface of the ontology, to close the open side of the storage tank, and accommodating cavity is formed, the appearance
Chamber is set for keeping culture medium, so that the zebra fish breathes.
6. a kind of fixing means of zebra fish, which is characterized in that include the following steps：
Zebra fish fixing device is provided, the zebra fish fixing device includes ontology, and the ontology includes working surface, the work
Fixing groove and storage tank are offered as surface, the fixing groove is connected to the storage tank, and the fixing groove is for fixing spot
The body of horse fish, the storage tank are used to house the head of the zebra fish, and the madial wall of the storage tank and the zebra
The head interval of fish；And
The body of the zebra fish is stuck in the fixing groove, and makes the head captured of the zebra fish in the storage tank
It is interior, the madial wall interval on the head of the zebra fish and the storage tank.
7. the fixing means of zebra fish according to claim 6, which is characterized in that the body of the zebra fish is stuck in institute
It states in fixing groove, and the head captured of the zebra fish is included the following steps in the step in the storage tank：
By the zebra fish and for being positioned over working surface for the culture medium of zebra fish breathing, the zebra fish is located at
In the culture medium；
The zebra fish is stirred using needle so that the body of the zebra fish and head respectively with the fixing groove and the appearance
Slot face is set, the back of the zebra fish is far from the working surface；And
The culture medium is siphoned away using blotting paper, when siphoning away the culture medium, the body of the zebra fish and head are suitable respectively
Gesture slides into the fixing groove and the storage tank.
8. the fixing means of zebra fish according to claim 6, which is characterized in that when the body of the zebra fish is stuck in institute
It states in fixing groove, the head captured of the zebra fish applies adhesion with viscous after in the storage tank, in the working surface
The body of zebra fish is stated in residence, and the zebra fish is avoided to move.
9. the fixing means of zebra fish according to claim 6, which is characterized in that when the body of the zebra fish is stuck in institute
It states in fixing groove, the head captured of the zebra fish is after in the storage tank, and on the working surface, lid sets coverslip,
To cover the head of the zebra fish.
10. the fixing means of zebra fish according to claim 9, which is characterized in that the coverslip closing is described accommodating
The open side of slot, and the accommodating cavity for keeping culture medium is formed, culture medium is instilled in accommodating cavity so that zebra fish breathes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810321543.1A CN108670485B (en) | 2018-04-11 | 2018-04-11 | Zebra fish fixing device and fixing method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810321543.1A CN108670485B (en) | 2018-04-11 | 2018-04-11 | Zebra fish fixing device and fixing method thereof |
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| CN112642500A (en) * | 2020-11-02 | 2021-04-13 | 中山大学 | Multi-sample zebra fish fry high-flux microfluidic chip, screening system and application of multi-sample zebra fish fry high-flux microfluidic chip |
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