CN108663254A - Automatic adjustment method, device and the dyeing machine system of reagent dyeing duration - Google Patents
Automatic adjustment method, device and the dyeing machine system of reagent dyeing duration Download PDFInfo
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- CN108663254A CN108663254A CN201810468368.9A CN201810468368A CN108663254A CN 108663254 A CN108663254 A CN 108663254A CN 201810468368 A CN201810468368 A CN 201810468368A CN 108663254 A CN108663254 A CN 108663254A
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- 238000004043 dyeing Methods 0.000 title claims abstract description 276
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 35
- 238000000034 method Methods 0.000 title claims abstract description 16
- 239000012128 staining reagent Substances 0.000 claims abstract description 107
- 238000012549 training Methods 0.000 claims description 41
- 238000012216 screening Methods 0.000 claims description 8
- 238000012545 processing Methods 0.000 claims description 4
- 239000000975 dye Substances 0.000 description 34
- 238000004040 coloring Methods 0.000 description 12
- WZUVPPKBWHMQCE-XJKSGUPXSA-N (+)-haematoxylin Chemical compound C12=CC(O)=C(O)C=C2C[C@]2(O)[C@H]1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-XJKSGUPXSA-N 0.000 description 5
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Natural products C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 230000007423 decrease Effects 0.000 description 3
- 235000013399 edible fruits Nutrition 0.000 description 3
- 210000001519 tissue Anatomy 0.000 description 3
- 238000013461 design Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000001575 pathological effect Effects 0.000 description 2
- 238000002791 soaking Methods 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
Abstract
The invention discloses a kind of Automatic adjustment method, device and the dyeing machine system of reagent dyeing duration, this method includes：The first dyeing duration and the second dyeing duration are increased separately after staining reagent is using preset times and using preset number of days；Obtain the actual use number and actual use number of days of staining reagent；When the actual use number of staining reagent is greater than or equal to using preset times, with the first dyeing cycle to being dyed when time sample to be dyed；When staining reagent is greater than or equal to using practical number of days using preset number of days, with the second dyeing cycle to being dyed when time sample to be dyed；And it is greater than or equal to using preset times and when being greater than or equal to using preset number of days using practical number of days of staining reagent, with the sum of the first dyeing cycle and the second dyeing cycle to being dyed when time sample to be dyed in the actual use number of staining reagent.The present invention can automatically gradually the added-time be dyed, and can either simplify operation, and can ensure dyeing quality.
Description
Technical field
The present invention relates to the field of medical instrument technology more particularly to a kind of Automatic adjustment method of reagent dyeing duration, dresses
It sets and dyeing machine system.
Background technology
Overflow dyeing machine is one of commonly used equipment in medical instrument, is mainly used for carrying out at dyeing biological tissue or cell
Reason, to facilitate the observation of biological tissue or cell.Traditional pathological staining machine, staining reagent can with the increase of tinction number,
Coloring continuously decreases.Assuming that fresh reagent concentration is 90%, then concentration will drop to 80% with access times increase
Left and right causes finally to organize observing effect in flakes under the microscope undesirable.The reagent soaking time of traditional overflow dyeing machine is all
It is changeless, coloring of batch sample can be thin after this is resulted in, this can influence pathological study person or medical worker couple
The analysis of cell or tissue；Generally use increases the replacement number of staining reagent and can be made if often replacing staining reagent in the industry
At reagent waste and increase manpower or production cost.
In view of this, it is necessary to which the automatic staining technology of current overflow dyeing machine is further improved in proposition.
Invention content
To solve an above-mentioned at least technical problem, the main object of the present invention is to provide a kind of multiple batches of sample dyeing duration
Automatic adjustment method.
To achieve the above object, one aspect of the present invention is：A kind of the automatic of reagent dyeing duration is provided
Adjusting method, including：
Increase by the first dyeing duration after staining reagent is using preset times, and after staining reagent is using preset number of days
Increase by the second dyeing duration；
Obtain the actual use number and actual use number of days of staining reagent；
When the actual use number of staining reagent is greater than or equal to using preset times, with the first dyeing cycle to when secondary
Sample to be dyed is dyed；
When staining reagent is greater than or equal to using practical number of days using preset number of days, with the second dyeing cycle to when secondary
Sample to be dyed is dyed；And it is greater than or equal to using preset times and dyeing examination in the actual use number of staining reagent
When being greater than or equal to using preset number of days using practical number of days of agent, with the sum of the first dyeing cycle and the second dyeing cycle to working as
Secondary sample to be dyed is dyed,
Wherein, the first dyeing cycle is the sum of initial dyeing cycle and the first dyeing duration；Second dyeing cycle is initial
The sum of dyeing cycle and the second dyeing cycle, third dyeing cycle are the first dyeing duration, the second dyeing duration and initial dyeing
The sum of period.
Wherein, described before increasing by the first dyeing duration after staining reagent is using preset times, further include：
Multiple coloration results are obtained after being dyed to multiple batches of training sample using same staining reagent；
It is greater than or equal to the training sample of dyeing threshold value and (n+1)th dyeing in the training sample dye number of n-th dyeing
When dye number is less than dyeing threshold value, use preset times of the n-th dyeing as staining reagent are selected, wherein n is to be more than or wait
In 1 natural number.
Wherein, it is described multiple batches of training sample is dyed using same staining reagent after obtain multiple coloration results it
Afterwards, further include：
The training sample dye number dyed at the m days is greater than or equal to the training sample of dyeability threshold value and dyeing in the m+1 days
When this dye number is less than dyeing threshold value, selectes dyeing in the m days and use preset number of days as staining reagent, wherein m to be more than or
Natural number equal to 1.
Wherein, described pair after time sample to be dyed dyes, further include：
The dyeing duration that record is dyed with the second dyeing cycle to working as time sample to be dyed；
The primitive period of dyeing is updated to the dyeing cycle after adjustment, and with the dyeing cycle after adjustment to waiting for next time
Color card is dyed.
In order to solve the above technical problems, another technical solution used in the present invention is：A kind of reagent dyeing duration is provided
Self-checking device, including：
Presetting module dyes duration for increasing by first after staining reagent uses preset times, and in staining reagent
Duration is dyed using increasing by second after preset number of days；
Acquisition module, the actual use number for obtaining staining reagent and actual use number of days；
Processing module, for when the actual use number in staining reagent is greater than or equal to using preset times, with the
One dyeing cycle when time sample to be dyed to dyeing；It is greater than or equal to using default using practical number of days in staining reagent
When number of days, with the second dyeing cycle to being dyed when time sample to be dyed；And it is big in the actual use number of staining reagent
In or equal to use when using practical number of days to be greater than or equal to use preset number of days of preset times and staining reagent, contaminated with first
The sum of color period and the second dyeing cycle are dyed to working as time sample to be dyed,
Wherein, the first dyeing cycle is the sum of initial dyeing cycle and the first dyeing duration；Second dyeing cycle is initial
The sum of dyeing cycle and the second dyeing cycle, third dyeing cycle are the first dyeing duration, the second dyeing duration and initial dyeing
The sum of period.
Wherein, further include：
Training module, for obtaining multiple dyeing knots after being dyed to multiple batches of training sample using same staining reagent
Fruit；
First screening module, the training sample dye number for being dyed in n-th are greater than or equal to dyeing threshold value and (n+1)th
When the training sample dye number of secondary dyeing is less than dyeing threshold value, use preset times of the n-th dyeing as staining reagent are selected,
Wherein, n is the natural number more than or equal to 1.
Wherein, further include：
Second screening module, the training sample dye number for being dyed at the m days are greater than or equal to dyeability threshold value and m
When the training sample dye number of dyeing in+1 day is less than dyeing threshold value, day is preset in the use for selecting dyeing in the m days as staining reagent
Number, wherein m is the natural number more than or equal to 1.
Wherein, further include：
Logging modle, for recording the dyeing duration dyed with the second dyeing cycle to working as time sample to be dyed；
Update module, for the primitive period of dyeing to be updated to the dyeing cycle after adjustment, and with the dyeing after adjustment
Period dyes sample to be dyed next time.
To achieve the above object, another technical solution that the present invention uses for：A kind of dyeing machine system is provided, including upper
The self-checking device for the reagent dyeing duration stated.
The problem that effect is partially shallow after staining reagent repeatedly dyes is different from the prior art in technical scheme of the present invention, using root
Added-time dyeing is carried out to the soaking time of sample according to the access times of staining reagent and using antenna, cumbersome operation can be reduced
While, and can ensure dyeing quality, reach the uniformity of multiple coloring, the chromosome of pathologist can be improved
It tests.
Description of the drawings
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technology description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
Some embodiments of invention for those of ordinary skill in the art without creative efforts, can be with
The structure shown according to these attached drawings obtains other attached drawings.
Fig. 1 is the method flow diagram of the Automatic adjustment method of one embodiment of the invention reagent dyeing duration；
Fig. 2 is the block diagram of the self-checking device of one embodiment of the invention reagent dyeing duration；
Fig. 3 is the design sketch that same staining reagent repeatedly dyes in the prior art；
Fig. 4 is the design sketch that the same staining reagent of the present invention repeatedly dyes.
The embodiments will be further described with reference to the accompanying drawings for the realization, the function and the advantages of the object of the present invention.
Specific implementation mode
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation describes, it is clear that described embodiment is only a part of the embodiment of the present invention, instead of all the embodiments.Base
Embodiment in the present invention, those of ordinary skill in the art obtained without creative efforts it is all its
His embodiment, shall fall within the protection scope of the present invention.
It is to be appreciated that the description of " first ", " second " etc. is used for description purposes only involved in the present invention, and should not be understood as
It indicates or implies its relative importance or implicitly indicate the quantity of indicated technical characteristic.Define as a result, " first ",
The feature of " second " can explicitly or implicitly include at least one of the features.In addition, the technical side between each embodiment
Case can be combined with each other, but must can be implemented as basis with those of ordinary skill in the art, when the combination of technical solution
Conflicting or cannot achieve when occur will be understood that the combination of this technical solution is not present, also not the present invention claims guarantor
Within the scope of shield.
Currently, when staining reagent repeatedly dyes, the concentration of staining reagent can be caused to reduce, be dyed using fixed dyeing time
When, staining reagent is different to the coloring of the sample of multiple batches.As shown in figure 3, with the increasing of staining reagent access times
Add, coloring is gradually thin out, influences the observation of pathology personnel.For this purpose, devising the automatic adjustment of following reagent dyeing durations
Method can make multiple coloring substantially balanced.
Fig. 1 is please referred to, in embodiments of the present invention, the Automatic adjustment method of the reagent dyeing duration, including：
Step S10, increase by the first dyeing duration after staining reagent is using preset times, and used in advance in staining reagent
If increasing by the second dyeing duration after number of days；In this step, it is contemplated that reagent concentration reduces after staining reagent repeatedly dyes, and passes through increasing
Add dyeing time, specifically increases by the first dyeing duration on the basis of original dyeing cycle, same staining reagent pair can be stablized
The coloring of multiple batches of sample.The reagent concentration influenced simultaneously in view of the volatilization of staining reagent reduces, and is contaminated by increasing
The color time, specifically the second dyeing of superposition duration on the basis of dyeing cycle, can stablize same staining reagent to multiple batches of sample
This coloring.
Step S20, the actual use number and actual use number of days of staining reagent are obtained；In this step, staining reagent
Actual use number and actual use number of days can be automatically recorded by equipment, can be according to actual use number when specifically judging
With preset times, and the relationship of actual use number of days and preset number of days, the increased dyeing time length of institute is determined.
Step S30, when the actual use number of staining reagent is greater than or equal to using preset times, with the first dyeing week
Phase when time sample to be dyed to dyeing；It is less than in the actual use number of staining reagent and uses preset times, initially contaminates
The color period when secondary to waiting for that sample dyes.
Step S40, when staining reagent is greater than or equal to using practical number of days using preset number of days, with the second dyeing week
Phase when time sample to be dyed to dyeing；When staining reagent is less than using practical number of days using preset number of days, with initial
Dyeing cycle when secondary to waiting for that sample dyes.
Step S50, being greater than or equal in the actual use number of staining reagent is made using preset times and staining reagent
When being greater than or equal to using preset number of days with practical number of days, with the sum of the first dyeing cycle and the second dyeing cycle to waiting contaminating when secondary
Colo(u)r atlas is dyed.
In for step S50, the actual use number for first comparing staining reagent may be used and using preset times
Size, the actual use number of days of rear relatively staining reagent can also use with using the size of preset number of days and first compare dyeing examination
Agent actually uses number of days with using the size of preset number of days, compares the actual use number of staining reagent afterwards and using default secondary
Several sizes.Wherein, the first dyeing cycle is the sum of initial dyeing cycle and the first dyeing duration；Second dyeing cycle is initial
The sum of dyeing cycle and the second dyeing cycle, third dyeing cycle are the first dyeing duration, the second dyeing duration and initial dyeing
The sum of period.
In a specific embodiment, it is described staining reagent using increase after preset times the first dyeing duration it
Before, further include：
Multiple coloration results are obtained after being dyed to multiple batches of training sample using same staining reagent；
It is greater than or equal to the training sample of dyeing threshold value and (n+1)th dyeing in the training sample dye number of n-th dyeing
When dye number is less than dyeing threshold value, use preset times of the n-th dyeing as staining reagent are selected, wherein n is to be more than or wait
In 1 natural number.In this step, since training sample repeatedly dyes so that staining reagent concentration continuously decreases, in n-th dyeing
When training sample dyeing angle value be in critical value and more than dyeing threshold value when, next batch sample dyeing needs to increase corresponding
Dyeing time, at this point, n-th dyeing is the use preset times of staining reagent.
Further, it is described multiple batches of training sample is dyed using same staining reagent after obtain multiple dyeing knot
After fruit, further include：
The training sample dye number dyed at the m days is greater than or equal to the training sample of dyeability threshold value and dyeing in the m+1 days
When this dye number is less than dyeing threshold value, selectes dyeing in the m days and use preset number of days as staining reagent, wherein m to be more than or
Natural number equal to 1.In this step, consider that factor of volatilizing when dyeing, dyeing in more days make staining reagent concentration continuously decrease,
When the dyeing angle value of the m times training sample is in critical value and is more than dyeing threshold value, next batch sample dyeing needs increase pair
The dyeing time answered, at this point, dyeing in the m days uses preset number of days for staining reagent.
In a specific embodiment, described pair after time sample to be dyed dyes, further include：
The dyeing duration that record is dyed with the second dyeing cycle to working as time sample to be dyed；
The primitive period of dyeing is updated to the dyeing cycle after adjustment, and with the dyeing cycle after adjustment to waiting for next time
Color card is dyed.In this step, in the multiple dyeing of same staining reagent, it can be arranged multiple using default time
Number.Being more than to be more than using the actual use number of days of preset times and/or staining reagent in the actual use number of staining reagent is made
It, can be in the first dyeing cycle, the second dyeing cycle or third dyeing cycle when being dyed with preset number of days next time
Any is the primitive period, is adjusted so as to the superposition of the dyeing time to subsequent samples.
To be better understood from this programme, it is below haematoxylin as example using staining reagent, illustrates：
After selecting haematoxylin option in reagent option, (it can be used by dip dyeing number of days (using number of days) or frame number respectively
Number) it is configured.
It is configured by frame number：Such as default frame number is set as every 2 framves, increases by one first dyeing duration, and specially 1
Second, then it represents that when haematoxylin use to 4 frame, the time of dip dyeing can increase by 1 second on the basis of the program setting time；Work as bush
When plain use to 7 frame, the time of dip dyeing can increase by 2 seconds on the basis of the program setting time, and so on.It is carried out by number of days
Setting：Such as preset number of days was set as every 2 days, one second dyeing duration of increase, specially 1 second.Then indicate that haematoxylin soaks
When dye was to the 4th day, the time of dip dyeing can increase by 1 second on the basis of the program setting time；When haematoxylin was disseminated to the 7th day,
The time of dip dyeing can increase by 2 seconds on the basis of the program setting time, and so on.Same staining reagent is made using n times
After m days, the dyeing time of next sample is the sum of original dyeing cycle and adjustment dyeing duration, and specific effect please refers to Fig. 4,
As can be seen from Figure 4 the 1st batch, the 50th batch, the 100th batch and the 150th batch coloring is roughly the same.
Please refer to Fig. 2, in the embodiment of the present invention, the self-checking device of the reagent dyeing duration, including：
Presetting module 10 is tried for increasing by the first dyeing duration after staining reagent uses preset times, and in dyeing
Agent increases by the second dyeing duration after using preset number of days；Reagent concentration reduces after repeatedly being dyed in view of staining reagent, passes through increasing
Add dyeing time, specifically increases by the first dyeing duration on the basis of original dyeing cycle, the dye of multiple batches of sample can be stablized
Color effect.The reagent concentration influenced simultaneously in view of the volatilization of staining reagent reduces, and by increasing dyeing time, specifically exists
The second dyeing of superposition duration, can stablize the coloring of multiple batches of sample on the basis of dyeing cycle.It should be understood that the use
It preset times and is configured according to the coloring of different reagents using preset number of days.
Acquisition module 20, the actual use number for obtaining staining reagent and actual use number of days；Pass through acquisition module
20, the actual use number and actual use number of days data of staining reagent are obtained, convenient follow-up adjustment is to when time dyeing of dyeing
Time.
Processing module 30, for when the actual use number of staining reagent is greater than or equal to using preset times, with the
One dyeing cycle when time sample to be dyed to dyeing；It is greater than or equal to using default using practical number of days in staining reagent
When number of days, with the second dyeing cycle to being dyed when time sample to be dyed；And it is big in the actual use number of staining reagent
In or equal to use when using practical number of days to be greater than or equal to use preset number of days of preset times and staining reagent, contaminated with first
The sum of color period and the second dyeing cycle when time sample to be dyed to dyeing.Staining reagent is adjusted by processing module 30
Dyeing time, so that the coloring of multiple batches of sample is balanced, above-mentioned, the first dyeing cycle is initial dyeing cycle and first
Dye the sum of duration；Second dyeing cycle is the sum of initial dyeing cycle and the second dyeing cycle, and third dyeing cycle is first
Dye duration, the second dyeing the sum of duration and initial dyeing cycle.
In a specific embodiment, further include：
Training module, for obtaining multiple dyeing knots after being dyed to multiple batches of training sample using same staining reagent
Fruit；
First screening module, the training sample dye number for being dyed in n-th are greater than or equal to dyeing threshold value and (n+1)th
When the training sample dye number of secondary dyeing is less than dyeing threshold value, use preset times of the n-th dyeing as staining reagent are selected,
Wherein, n is the natural number more than or equal to 1.By training module and the first screening module, the use of staining reagent can be selected
Preset times.
Further, further include：
Second screening module, the training sample dye number for being dyed at the m days are greater than or equal to dyeability threshold value and m
When the training sample dye number of dyeing in+1 day is less than dyeing threshold value, day is preset in the use for selecting dyeing in the m days as staining reagent
Number, wherein m is the natural number more than or equal to 1.By training module and the second screening module, staining reagent can be selected
Use preset number of days；
In a specific embodiment, further include：
Logging modle, for recording the dyeing duration dyed with the second dyeing cycle to working as time sample to be dyed；
Update module, for the primitive period of dyeing to be updated to the dyeing cycle after adjustment, and with the dyeing after adjustment
Period dyes sample to be dyed next time.Be more than using preset times in the actual use number of staining reagent and/or
The actual use number of days of staining reagent is more than when being dyed using preset number of days next time, can be by update module with first
In dyeing cycle, the second dyeing cycle or third dyeing cycle it is any be the primitive period, when so as to the dyeing of subsequent samples
Between superposition adjustment.
In the embodiment of the present invention, which includes the self-checking device of above-mentioned reagent dyeing duration, examination
The function and structure of the self-checking device of agent dyeing duration please refer to above-described embodiment, and details are not described herein again.Due to overflow dyeing machine
System applies the self-checking device of above-mentioned reagent dyeing duration, therefore has the advantages that its all and effect.
The foregoing is merely the preferred embodiment of the present invention, are not intended to limit the scope of the invention, every at this
Under the inventive concept of invention, using equivalent structure transformation made by description of the invention and accompanying drawing content, or directly/use indirectly
In the scope of patent protection that other related technical areas are included in the present invention.
Claims (9)
1. a kind of Automatic adjustment method of reagent dyeing duration, which is characterized in that the automatic adjustment side of the reagent dyeing duration
Method includes：
Increase by the first dyeing duration after staining reagent is using preset times, and increases after staining reagent is using preset number of days
Second dyeing duration；
Obtain the actual use number and actual use number of days of staining reagent；
When the actual use number of staining reagent is greater than or equal to using preset times, with the first dyeing cycle to waiting contaminating when secondary
Colo(u)r atlas is dyed；
When staining reagent is greater than or equal to using practical number of days using preset number of days, with the second dyeing cycle to waiting contaminating when secondary
Colo(u)r atlas is dyed；And
It is greater than or equal in the actual use number of staining reagent big using the practical number of days of use of preset times and staining reagent
When using preset number of days, with the sum of the first dyeing cycle and the second dyeing cycle to being contaminated when time sample to be dyed
Color,
Wherein, the first dyeing cycle is the sum of initial dyeing cycle and the first dyeing duration；Second dyeing cycle is initial dyeing
The sum of period and the second dyeing cycle, third dyeing cycle are the first dyeing duration, the second dyeing duration and initial dyeing cycle
The sum of.
2. the Automatic adjustment method of reagent dyeing duration as described in claim 1, which is characterized in that described to make in staining reagent
With before increasing by the first dyeing duration after preset times, further include：
Multiple coloration results are obtained after being dyed to multiple batches of training sample using same staining reagent；
It is greater than or equal to the training sample dyeing of dyeing threshold value and (n+1)th dyeing in the training sample dye number of n-th dyeing
When value is less than dyeing threshold value, use preset times of the n-th dyeing as staining reagent are selected, wherein n is more than or equal to 1
Natural number.
3. the Automatic adjustment method of reagent dyeing duration as claimed in claim 2, which is characterized in that described to utilize same dyeing
Reagent obtains multiple coloration results after being dyed to multiple batches of training sample after, further include：
The training sample dye number dyed at the m days is greater than or equal to dyeability threshold value and the training sample dye of dyeing in the m+1 days
Color value be less than dyeing threshold value when, select the m days dyeing as staining reagent use preset number of days, wherein m for more than or equal to
1 natural number.
4. the Automatic adjustment method of reagent dyeing duration as claimed in claim 3, which is characterized in that described pair when secondary to be dyed
After sample is dyed, further include：
The dyeing duration that record is dyed with the second dyeing cycle to working as time sample to be dyed；
The primitive period of dyeing is updated to the dyeing cycle after adjustment, and with the dyeing cycle after adjustment to next time to be dyed
Sample is dyed.
5. a kind of self-checking device of reagent dyeing duration, which is characterized in that the automatic adjustment of the reagent dyeing duration fills
Set including：
Presetting module is used for increasing by the first dyeing duration after staining reagent uses preset times, and in staining reagent
Increase by the second dyeing duration after preset number of days；
Acquisition module, the actual use number for obtaining staining reagent and actual use number of days；
Processing module, for when the actual use number in staining reagent is greater than or equal to using preset times, being contaminated with first
The color period when time sample to be dyed to dyeing；It is greater than or equal to using practical number of days in staining reagent and uses preset number of days
When, with the second dyeing cycle to being dyed when time sample to be dyed；And be more than in the actual use number of staining reagent or
Equal to preset times and when using practical number of days more than or equal to preset number of days is used of staining reagent is used, with the first dyeing week
The sum of phase and the second dyeing cycle are dyed to working as time sample to be dyed,
Wherein, the first dyeing cycle is the sum of initial dyeing cycle and the first dyeing duration；Second dyeing cycle is initial dyeing
The sum of period and the second dyeing cycle, third dyeing cycle are the first dyeing duration, the second dyeing duration and initial dyeing cycle
The sum of.
6. the self-checking device of reagent dyeing duration as claimed in claim 5, which is characterized in that further include：
Training module, for obtaining multiple coloration results after being dyed to multiple batches of training sample using same staining reagent；
First screening module, the training sample dye number for being dyed in n-th are greater than or equal to dyeing threshold value and (n+1)th dye
When the training sample dye number of color is less than dyeing threshold value, use preset times of the n-th dyeing as staining reagent are selected,
In, n is the natural number more than or equal to 1.
7. the self-checking device of reagent dyeing duration as claimed in claim 6, which is characterized in that further include：
Second screening module, the training sample dye number for being dyed at the m days are greater than or equal to dyeability threshold value and the m+1 days
When the training sample dye number of dyeing is less than dyeing threshold value, selectes dyeing in the m days and uses preset number of days as staining reagent,
In, m is the natural number more than or equal to 1.
8. the self-checking device of reagent dyeing duration as claimed in claim 7, which is characterized in that further include：
Logging modle, for recording the dyeing duration dyed with the second dyeing cycle to working as time sample to be dyed；
Update module, for the primitive period of dyeing to be updated to the dyeing cycle after adjustment, and with the dyeing cycle after adjustment
Sample to be dyed next time is dyed.
9. a kind of dyeing machine system, which is characterized in that the dyeing machine system includes such as claim 5 to 8 any one of them
The self-checking device of reagent dyeing duration.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810468368.9A CN108663254A (en) | 2018-05-16 | 2018-05-16 | Automatic adjustment method, device and the dyeing machine system of reagent dyeing duration |
Applications Claiming Priority (1)
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| CN111028929A (en) * | 2019-11-29 | 2020-04-17 | 广州金域医学检验中心有限公司 | Management method and device for section staining, computer equipment and storage medium |
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