CN108659070A - A kind of preparation method of hyperin - Google Patents
A kind of preparation method of hyperin Download PDFInfo
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- CN108659070A CN108659070A CN201710212135.8A CN201710212135A CN108659070A CN 108659070 A CN108659070 A CN 108659070A CN 201710212135 A CN201710212135 A CN 201710212135A CN 108659070 A CN108659070 A CN 108659070A
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- hyperin
- film
- cavitation
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- dried
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
- C07H1/08—Separation; Purification from natural products
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H17/00—Compounds containing heterocyclic radicals directly attached to hetero atoms of saccharide radicals
- C07H17/04—Heterocyclic radicals containing only oxygen as ring hetero atoms
- C07H17/06—Benzopyran radicals
- C07H17/065—Benzo[b]pyrans
- C07H17/07—Benzo[b]pyran-4-ones
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- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
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- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicinal Preparation (AREA)
Abstract
The present invention relates to a kind of preparation methods of hyperin, and in particular to a method of hyperin in tassel flower is extracted using multiple-effect broken wall cryotronl collimation method.Its processing step includes：Tassel flower is dried in the shade, is crushed, by raw material by ultra high pressure extraction device, is extracted with entering percussion flow after the pressure extraction of 100MPa, carries out cavitation subsequently into cavitation extraction device, 100% cell wall passes through supersonic jet extractor after dismembering；Extracting solution is entered into film after press filtration drainage piece-rate system and is combined multistage film separation system, enters resin column piece-rate system later, obtains separating liquid；By separating liquid through film combination Multistage Membranes concentration systems concentration, concentrate is obtained；Gained concentrate is dried through vacuum belt-type dryer finally, obtains hyperin.The present invention has labor intensity low, and operating environment is excellent.The advantages that room temperature is run, and heat-sensitive ingredients are decomposed without destruction, and color, smell and taste reservation is abundant and environmental-friendly, and circular agriculture comprehensive utilization ratio is high.
Description
Technical field
The present invention relates to a kind of preparation methods of hyperin, belong to traditional Chinese medicine extyaction field.
Background technology
Tassel flower alias leaf lower red, sowthistle tasselflower herb, redback chrismashush root and leaf, 72 flowers, false cabbage mustard, goatweed, day hair grass, wild mustard
The red grass of orchid, oxtail knee, loudspeaker, sheep hoof angle, mengtzu peperomia.According to analysis, tassel flower contains hyperin.
Hyperoside also known as Quercetin -3-O- β-D- galactopyranosides.Belong to flavonols glycosides compound.Aglycon is
Quercetin, glycosyl are galactopyranose, are connected with glycosyl with β glycosidic bonds by 3 O atoms of Quercetin.Hyperoside distribution is wide
It is general, there is anti-inflammatory, spasmolysis, diuresis, cough-relieving, decompression, reduce cholesterol, protein assimilating, part and maincenter town pain and to the heart, brain
A variety of physiological activity such as the protective effect of blood vessel are a kind of important natural products.Since its raw material tassel flower is distributed more widely, from
Middle extraction hyperin has achieved encouraging progress.
Hyperin extracts in preparation process, usually refluxing extraction, big sky resin adsorption, but these methods are remaining with
Imitate ingredient, removal invalid components etc., there is energy consumptions it is big, loss of effective components is big the shortcomings of.
Invention content
The present invention be directed to the deficiencies in the prior art, it is desirable to provide a kind of high efficiency and selective, easy to operate, yield
It is high, by-product is few, can realization of industrialization the method for preparing hyperin.
Technical scheme of the present invention is carried out according to the following steps：
A. tassel flower is dried in the shade, is crushed, by raw material by ultra high pressure extraction device, hit with entering after the pressure extraction of 100MPa
Stream extraction is hit, carries out cavitation subsequently into cavitation extraction device, 100% cell wall is carried after dismembering by supersonic jet
Take device；
B., extracting solution is entered to film combination-multistage film separation system after press filtration drainage piece-rate system, later into resin column point
From system, separating liquid is obtained；Separating liquid is concentrated through film combination-Multistage Membranes concentration systems, obtains concentrate；
C. gained concentrate is dried through vacuum belt-type dryer, obtains hyperin.
Ultra high pressure extraction device pressure described in step a is 100MPa, power of motor 75KW.After cavitation comprehensive function,
100% cell wall is dismembered, about 20 microns of fragment, and secondary metabolite is directly contacted with solvent, is acted on and was transported without osmotic pressure
Journey, solvents counter-current extraction very high speed are nearly 10 times of velocity of sound.
Ceramic membrane permeate turbidity is less than or equal to 1 in film combination-multistage film separation system described in step b, film concentration
The pure pressure-driven of equipment concentration process, flow of inlet water 6.5m3/h。
Vacuum belt-type dryer drying and dehydrating ultrahigh vacuum described in step c, and starvation, rate of drying is fast, steams
Hair amount is big and good product quality.
The advantage of the invention is that：
1. the present invention handles raw material using multiple-effect broken wall cryogenic pipelines extraction method, realizes crushing, extracts step completion, while by
In entire production process without heating, produced in cryogenic pipelines, so as to effectively avoid the destruction of hyperin ingredient.
2. of the invention use multiple-effect broken wall cryogenic pipelines to extract hyperin, there is quick, efficient, recovery rate height, completely
The advantages of retaining hyperin active ingredient.
It is further illustrated the present invention below in conjunction with specific implementation mode, but the scope of protection of present invention is not limited to
In following embodiments.
Specific implementation mode：
Embodiment 1：
Tassel flower is dried in the shade, is crushed, by raw material by ultra high pressure extraction device, to enter percussion flow after the pressure extraction of 100MPa
Extraction carries out cavitation subsequently into cavitation extraction device, and 100% cell wall passes through supersonic jet extractor after dismembering；
Extracting solution is entered into film combination-multistage film separation system after press filtration drainage piece-rate system, enters resin column piece-rate system later,
Obtain separating liquid；Separating liquid is concentrated through film combination-Multistage Membranes concentration systems, ceramic membrane is saturating in film combination-multistage film separation system
Cross liquid turbidity be less than or equal to 1, the pure pressure-driven of film concentrator concentration process, flow of inlet water 6m3/ h, obtains concentrate；Finally
Gained concentrate is dried through vacuum belt-type dryer, hyperin is obtained, is detected through HPLC, purity 98.2%.
Embodiment 2：
Tassel flower is dried in the shade, is crushed, by raw material by ultra high pressure extraction device, to enter percussion flow after the pressure extraction of 100MPa
Extraction carries out cavitation subsequently into cavitation extraction device, and 100% cell wall passes through supersonic jet extractor after dismembering；
Extracting solution is entered into film combination-multistage film separation system after press filtration drainage piece-rate system, enters resin column piece-rate system later,
Obtain separating liquid；Separating liquid is concentrated through film combination-Multistage Membranes concentration systems, ceramic membrane is saturating in film combination-multistage film separation system
Cross liquid turbidity be less than or equal to 1, the pure pressure-driven of film concentrator concentration process, flow of inlet water 6.5m3/ h, obtains concentrate；Most
Gained concentrate is dried through vacuum belt-type dryer afterwards, hyperin is obtained, is detected through HPLC, purity 98.5%.
Embodiment 3：
Tassel flower is dried in the shade, is crushed, by raw material by ultra high pressure extraction device, to enter percussion flow after the pressure extraction of 100MPa
Extraction carries out cavitation subsequently into cavitation extraction device, and 100% cell wall passes through supersonic jet extractor after dismembering；
Extracting solution is entered into film combination-multistage film separation system after press filtration drainage piece-rate system, enters resin column piece-rate system later,
Obtain separating liquid；Separating liquid is concentrated through film combination-Multistage Membranes concentration systems, ceramic membrane is saturating in film combination-multistage film separation system
Cross liquid turbidity be less than or equal to 1, the pure pressure-driven of film concentrator concentration process, flow of inlet water 7m3/ h, obtains concentrate；Finally
Gained concentrate is dried through vacuum belt-type dryer, hyperin is obtained, is detected through HPLC, purity 97.9%.
Claims (4)
1. a kind of preparation method of hyperin, it is characterised in that：
A. tassel flower is dried in the shade, is crushed, by raw material by ultra high pressure extraction device, hit with entering after the pressure extraction of 100MPa
Stream extraction is hit, carries out cavitation subsequently into cavitation extraction device, 100% cell wall is carried after dismembering by supersonic jet
Take device；
B., extracting solution is entered to film combination-multistage film separation system after press filtration drainage piece-rate system, later into resin column point
From system, separating liquid is obtained；Separating liquid is concentrated through film combination-Multistage Membranes concentration systems, obtains concentrate；
C. gained concentrate is dried through vacuum belt-type dryer, obtains hyperin.
2. a kind of preparation method of hyperin according to claim 1, which is characterized in that the superelevation described in step a
Pressure extractor pressure is 100MPa, power of motor 75KW, and after cavitation comprehensive function, 100% cell wall is dismembered, and fragment about 20 is micro-
Rice, secondary metabolite are directly contacted with solvent, and transport process, solvents counter-current extraction speed pole are acted on without osmotic pressure
Height is nearly 10 times of velocity of sound.
3. a kind of preparation method of hyperin according to claim 1, which is characterized in that the film connection described in step b
It is less than or equal to 1 with ceramic membrane permeate turbidity in-multistage film separation system, the pure pressure-driven of film concentrator concentration process, into
Water flow is 6.5m3/h。
4. a kind of preparation method of hyperin according to claim 1, which is characterized in that the vacuum described in step c
Belt dryer drying and dehydrating ultrahigh vacuum, and starvation, rate of drying is fast, and evaporation capacity is big and good product quality.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710212135.8A CN108659070A (en) | 2017-04-01 | 2017-04-01 | A kind of preparation method of hyperin |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710212135.8A CN108659070A (en) | 2017-04-01 | 2017-04-01 | A kind of preparation method of hyperin |
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| Publication Number | Publication Date |
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| CN108659070A true CN108659070A (en) | 2018-10-16 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201710212135.8A Pending CN108659070A (en) | 2017-04-01 | 2017-04-01 | A kind of preparation method of hyperin |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110655997A (en) * | 2019-10-21 | 2020-01-07 | 青岛啤酒股份有限公司 | Preparation method of lophatherum gracile health beer |
-
2017
- 2017-04-01 CN CN201710212135.8A patent/CN108659070A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110655997A (en) * | 2019-10-21 | 2020-01-07 | 青岛啤酒股份有限公司 | Preparation method of lophatherum gracile health beer |
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| PB01 | Publication | ||
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20181016 |