CN108645900A - A kind of preparation method for the glutathione modified electrode can be applied to electrochemistry chiral Recognition - Google Patents
A kind of preparation method for the glutathione modified electrode can be applied to electrochemistry chiral Recognition Download PDFInfo
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- CN108645900A CN108645900A CN201810346999.3A CN201810346999A CN108645900A CN 108645900 A CN108645900 A CN 108645900A CN 201810346999 A CN201810346999 A CN 201810346999A CN 108645900 A CN108645900 A CN 108645900A
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- glutathione
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/308—Electrodes, e.g. test electrodes; Half-cells at least partially made of carbon
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- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
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- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/48—Systems using polarography, i.e. measuring changes in current under a slowly-varying voltage
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Abstract
The present invention relates to a kind of preparation methods for the glutathione modified electrode can be applied to electrochemistry chiral Recognition.Include the following steps：It prepares glutathione solution, prepare glutathione modified electrode, electrochemical process identification amino acid enantiomer.The beneficial effects of the invention are as follows：The preparation method of glutathione modified electrode is simple and environmentally-friendly；And since there is glutathione certain chiral environment, glutathione modified electrode to have preferable recognition capability to amino acid enantiomer.
Description
Technical field
The present invention relates to a kind of preparation methods for the glutathione modified electrode can be applied to electrochemistry chiral Recognition, belong to
Biotechnology and electrochemical field.
Technical background
The chiral Recognition of amino acid all has the fields such as pharmaceutical chemistry, biomedicine, food analysis and materials chemistry
Extremely important researching value.Chiral Recognition refers to that chiral selector is specifically combined with the chiral isomer of a certain configuration, from
And it realizes chiral isomers and distinguishes.It is also completely different to the recognition effect of isomers due to the difference of chiral selector,
So the selection of chiral selective agent is just particularly important.Recently, our team are it has been reported that be based on phenylalanine dipeptide
Chiral interface carries out amino acid enantiomer the work of electrochemistry chiral Recognition.However, as far as we know, using simple biology
The progress that unit such as amino acid, peptide and protein carry out electrochemistry chiral Recognition is little.
Biologically active polypeptide drug glutathione contains a variety of bases such as sulfydryl, amino, carboxyl, amide groups
Group, is easily formed a variety of non-covalent bonds.Have pertinent literature report to the detection of glutathione, but using glutathione as hand
Property selective agent not yet had been reported that applied to electrochemical recognition amino acid, therefore this novel chiral identification system exploitation have emphatically
The economic value and social benefit wanted.
Invention content
The purpose of the present invention is to provide a kind of systems for the glutathione modified electrode can be applied to electrochemistry chiral Recognition
Preparation Method.Amino acid enantiomer can will be effectively identified after glutathione modification to glassy carbon electrode surface.
A kind of preparation method for the glutathione modified electrode can be applied to electrochemistry chiral Recognition, includes the following steps：
A, glutathione solution is prepared：It weighs a certain amount of glutathione to be added in the centrifuge tube of 5mL, be pipetted with pipette
The ultrapure water dissolutions of 4mL, after product fully dissolves, you can acquire certain density glutathione solution；
B, glutathione modified electrode is prepared：It is added dropwise to the liquid-transfering gun removing step a glutathione solutions being prepared
Glassy carbon electrode surface is incubated certain time at a certain temperature, you can obtains corresponding glutathione modified electrode；
C, electrochemical process identifies amino acid enantiomer：Amino acid enantiomer is identified using differential pulse voltammetry, by paddy
The sweet peptide modified electrode of Guang is statically placed in certain time in 20~30mL amino acid enantiomer solution, in the electrochemical window of 0.4~1.2V
Differential pulse voltammetry figure is recorded in mouth range, after having surveyed every time, modified electrode is 6~8 in 20~30mL, 0.1~0.3M pH
It is swept in phosphate buffer solution surely to restore electrode activity；
Further, a concentration of 0.4~1.6mM of step a Glutathiones peptide solution.
Further, the volume for the glutathione solution that liquid-transfering gun pipettes in step b is 2~8 μ L.
Further, incubation temperature is 0~6oC in step b.
Further, incubation time is 4~12h in step d.
Further, amino acid enantiomer is tryptophan, tyrosine and phenylalanine in step c.
Further, the concentration of amino acid enantiomer is 0.1~1.0mM in step c.
Further, time of repose is 30~90s in step c.
The beneficial effects of the invention are as follows：The preparation method of glutathione solution is simple and environmentally-friendly；And since glutathione has
Certain chiral environment, glutathione modified electrode have preferable recognition capability to amino acid enantiomer.
Description of the drawings
This experiment is further illustrated below in conjunction with the accompanying drawings.
Fig. 1 is the cyclic voltammogram of two GSH-PX activity modified electrode of embodiment.
Fig. 2 is the recognition effect figure of three GSH-PX activity modified electrode tryptophan enantiomer of embodiment.
Fig. 3 is recognition effect figure of the three GSH-PX activity modified electrode of embodiment to tyrosine enantiomer.
Fig. 4 is recognition effect figure of the three GSH-PX activity modified electrode of embodiment to phenylalanine enantiomer.
Specific implementation mode
Presently in connection with specific embodiment, the present invention will be further described, following embodiment be intended to illustrate invention rather than
Limitation of the invention further.
Amino acid enantiomer is identified in glutathione modified electrode of the present invention as follows：
RL/D=IL/ID
In formula, RL/DIndicate amino acid enantiomer oxidation peak current ratio, ILAnd IDL-type amino acid and D type ammonia are indicated respectively
Oxidation peak current value of the base acid on differential pulse voltammetry figure.
Embodiment one：
The preparation of glutathione solution includes following steps：
(1) it weighs 1mg glutathione to be added in the centrifuge tube of 5mL, pipettes the ultrapure water dissolutions of 4mL with pipette, wait for product
Fully after dissolving, you can obtain the glutathione solution of a concentration of 0.8mM.
(2) glutathione solution that the preparation of 5 μ L steps (1) is pipetted with liquid-transfering gun is added dropwise to glassy carbon electrode surface, at 4 DEG C
It is incubated 6h, you can obtain corresponding glutathione modified electrode.
Embodiment two：
The glutathione modified electrode that embodiment one is prepared is rested in 5mM potassium ferricyanide solutions, -0.2~
The modified electrode is characterized using cyclic voltammetry under the electrochemical window of 0.6V, it is 0.1V/s to sweep speed, and result is for example attached
It is reversible symmetrical cyclic voltammogram shown in Fig. 1, shown in glutathione modified electrode.
Embodiment three：
The glutathione modified electrode that embodiment one is prepared is rested on to the amino acid enantiomer (color of 25mL 0.5mM
Propylhomoserin, tyrosine and phenylalanine) in solution, difference arteries and veins is recorded within the scope of the electrochemical window of 0.4~1.2V after standing 60s
Voltammogram is rushed, after having surveyed every time, modified electrode is swept surely in the 0.1M phosphate buffer solutions of 25mL pH=7 to restore electrode
Activity.Attached drawing 2 is the recognition effect figure of glutathione modified electrode tryptophan enantiomer, and oxidation peak current ratio is 2.01.
Attached drawing 3 is identification of the glutathione modified electrode to tyrosine enantiomer, and oxidation peak current ratio is 1.91.Attached drawing 4 is paddy Guang
Sweet peptide modified electrode is to the recognition effect figure of phenylalanine enantiomer, and oxidation peak current ratio is 1.45, and recognition effect is minimum.
Claims (4)
1. a kind of preparation method for the glutathione modified electrode can be applied to electrochemistry chiral Recognition, steps are as follows：
A, glutathione solution is prepared：It weighs a certain amount of glutathione to be added in the centrifuge tube of 5mL, 4mL is pipetted with pipette
Ultrapure water dissolution, after product fully dissolves, you can obtain certain density glutathione solution；
B, glutathione modified electrode is prepared：It is added dropwise to glass carbon with the liquid-transfering gun removing step a glutathione solutions being prepared
Electrode surface is incubated certain time at a certain temperature, you can obtains corresponding glutathione modified electrode；
C, electrochemical process identifies amino acid enantiomer：Amino acid enantiomer is identified using differential pulse voltammetry, by gluathione
Peptide modified electrode is statically placed in certain time in 20~30mL amino acid enantiomer solution, in the electrochemical window model of 0.4~1.2V
Interior record differential pulse voltammetry figure is enclosed, the phosphoric acid that modified electrode is 6~8 in 20~30mL, 0.1~0.3M pH after having surveyed every time
It is swept in salt buffer solution surely to restore electrode activity.
2. a kind of preparation side for the glutathione modified electrode can be applied to electrochemistry chiral Recognition according to claim 1
Method, it is characterized in that：A concentration of 0.4~1.6mM of the step a Glutathione peptide solutions.
3. a kind of preparation side for the glutathione modified electrode can be applied to electrochemistry chiral Recognition according to claim 1
Method, it is characterized in that：The volume for the glutathione solution that liquid-transfering gun pipettes in the step b is 2~8 μ L, and incubation temperature is 0~6
DEG C, incubation time is 4~12h.
4. a kind of preparation side for the glutathione modified electrode can be applied to electrochemistry chiral Recognition according to claim 1
Method, it is characterized in that：Amino acid enantiomer is tryptophan, tyrosine and phenylalanine enantiomer, amino acid mapping in the step c
The concentration of body is 0.1~1.0mM, and time of repose of the glutathione modified electrode in amino acid enantiomer solution be 30~
90s。
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110243897A (en) * | 2019-07-10 | 2019-09-17 | 常州大学 | A kind of preparation of polyethyleneimine/chirality peptide modified glassy carbon electrode and its application in Tryptophan enantiomer Selective recognition |
| CN110455880A (en) * | 2019-07-02 | 2019-11-15 | 常州大学 | A kind of L lysine HCL can be used for electrochemistry chiral Recognition induces the preparation method of four pairs of carboxyl phenyl porphyrin self-assemblies |
| CN110658245A (en) * | 2019-10-28 | 2020-01-07 | 常州大学 | Preparation method of glutathione-double nano-metal particle composite material modified electrode capable of being applied to electrochemical chiral recognition |
| CN111537581A (en) * | 2020-05-13 | 2020-08-14 | 常州大学 | Preparation of electrochemical sensor for identifying configuration of non-electroactive enantiomer |
| CN114152655A (en) * | 2021-12-31 | 2022-03-08 | 常州大学 | Polystyrene and L-Phe compound modified electrode for chiral recognition of glutamic acid enantiomer and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104820005A (en) * | 2015-04-23 | 2015-08-05 | 常州大学 | Chiral sensor based on chitosan/cyclodextrin dinuclear copper and manufacturing method of chiral sensor |
| CN105044178A (en) * | 2015-04-23 | 2015-11-11 | 常州大学 | Chiral recognition to tryptophan enantiomer containing zinc ion by chiral sensor based on chitosan/sodium alginate |
| CN106226381A (en) * | 2016-07-11 | 2016-12-14 | 常州大学 | The preparation of a kind of Thermo-sensitive quinine chiral sensor and be applied to electrochemical recognition Tryptophan enantiomer |
-
2018
- 2018-04-18 CN CN201810346999.3A patent/CN108645900A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104820005A (en) * | 2015-04-23 | 2015-08-05 | 常州大学 | Chiral sensor based on chitosan/cyclodextrin dinuclear copper and manufacturing method of chiral sensor |
| CN105044178A (en) * | 2015-04-23 | 2015-11-11 | 常州大学 | Chiral recognition to tryptophan enantiomer containing zinc ion by chiral sensor based on chitosan/sodium alginate |
| CN106226381A (en) * | 2016-07-11 | 2016-12-14 | 常州大学 | The preparation of a kind of Thermo-sensitive quinine chiral sensor and be applied to electrochemical recognition Tryptophan enantiomer |
Non-Patent Citations (1)
| Title |
|---|
| LICONG ZHAO ET.AL: "Silica-based polypeptide-monolithic stationary phase for hydrophilic chromatography and chiral separation", 《JOURNAL OF CHROMATOGRAPHY A》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110455880A (en) * | 2019-07-02 | 2019-11-15 | 常州大学 | A kind of L lysine HCL can be used for electrochemistry chiral Recognition induces the preparation method of four pairs of carboxyl phenyl porphyrin self-assemblies |
| CN110455880B (en) * | 2019-07-02 | 2021-11-09 | 常州大学 | Preparation method of L-lysine hydrochloride induced tetra-p-carboxyphenylporphyrin self-assembly for electrochemical chiral recognition |
| CN110243897A (en) * | 2019-07-10 | 2019-09-17 | 常州大学 | A kind of preparation of polyethyleneimine/chirality peptide modified glassy carbon electrode and its application in Tryptophan enantiomer Selective recognition |
| CN110658245A (en) * | 2019-10-28 | 2020-01-07 | 常州大学 | Preparation method of glutathione-double nano-metal particle composite material modified electrode capable of being applied to electrochemical chiral recognition |
| CN111537581A (en) * | 2020-05-13 | 2020-08-14 | 常州大学 | Preparation of electrochemical sensor for identifying configuration of non-electroactive enantiomer |
| CN114152655A (en) * | 2021-12-31 | 2022-03-08 | 常州大学 | Polystyrene and L-Phe compound modified electrode for chiral recognition of glutamic acid enantiomer and preparation method thereof |
| CN114152655B (en) * | 2021-12-31 | 2023-11-14 | 常州大学 | Polystyrene and L-Phe compound modified electrode for chiral recognition of glutamic acid enantiomer and preparation method thereof |
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Application publication date: 20181012 |