CN108642052A - A kind of automation molecular detector arrangement - Google Patents
A kind of automation molecular detector arrangement Download PDFInfo
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- CN108642052A CN108642052A CN201810747165.3A CN201810747165A CN108642052A CN 108642052 A CN108642052 A CN 108642052A CN 201810747165 A CN201810747165 A CN 201810747165A CN 108642052 A CN108642052 A CN 108642052A
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Abstract
A kind of automation molecular detector arrangement, including：Portable inspectiont platform, is equipped with multigroup sense channel that one is extracted and detected for molecule, and each sense channel is equipped with the single detection as unit of each detection and encapsulates reagent device；Thermostat, the temperature needed for providing different extractions and amplification are placed on to thermostat variable below single detection encapsulation reagent device；Magnetic adsorption device, the magnetic field environment needed for providing different extractions and amplification are placed on to magnetic adsorption device variable below single detection encapsulation reagent device；Multiple channel mechanical arm is corresponded with sense channel, and multiple channel mechanical arm carries out pipetting to the single detection encapsulation reagent device on corresponding sense channel simultaneously, completes the extraction and detection of molecule；Fluorescence reading device is for the amplified production in liquid after Detection and Extraction.By molecule extraction and detection fusion in an equipment, the falsehood of manual operation generation and the risk to personnel, environment are farthest avoided.
Description
Technical field
The present invention relates to field of molecular detection, and in particular to a kind of automation molecular detector arrangement.
Background technology
It in molecule experiments, often needs to extract purifying and augmentation detection to the nucleic acid in sample, the Process Design is real
It is more complex to test operation, and manual operation has larger mistakes and omissions risk and has potential risk to the safety of experimenter, therefore needs certainly
Dynamicization instrument replaces manually being operated.It is largely single detection project, larger in existing molecule extraction and detection device
Detect the experimental design of flux, generally existing volume weight is big, malfunction, difficult shift position the problems such as, in experiment item
It is inconvenient for use when mesh is more, detection limit is less.
Invention content
The application provides a kind of automation molecular detector arrangement, including：
Portable inspectiont platform, is equipped with multigroup sense channel that one is extracted and detected for molecule, and each detection is logical
Road is equipped with the single detection as unit of each detection and encapsulates reagent device；
Thermostat, the temperature needed for providing different extractions and amplification, is placed on to thermostat variable single
Below detection encapsulation reagent device；
Magnetic adsorption device, the magnetic field environment needed for providing different extractions and amplification, puts to magnetic adsorption device variable
It sets below single detection encapsulation reagent device；
Multiple channel mechanical arm is corresponded with sense channel, and multiple channel mechanical arm is simultaneously to the list on corresponding sense channel
A detection encapsulation reagent device carries out pipetting, completes the extraction and detection of molecule；
Fluorescence reading device, for detecting the amplified production after multiple channel mechanical arm extracts in liquid.
In a kind of embodiment, single detection encapsulation trial assembly is set single including conventional reagent preassembled unit and special reagent prepackage
It is positioned on sense channel to member, conventional reagent preassembled unit and the splicing of special reagent preassembled unit；
If conventional reagent preassembled unit includes equipped with all reagents for providing molecule extraction and augmentation detection process needs
Dry test tube；
Special reagent preassembled unit includes that the test tube of molecule extracting solution and internal standard mixed liquor is housed and enzyme is housed and is sealed with wax
Test tube.
In a kind of embodiment, conventional reagent preassembled unit detachably splices with special reagent preassembled unit.
There are two test tube it is at least empty test tube in several test tubes in conventional reagent preassembled unit in a kind of embodiment.
In a kind of embodiment, thermostat includes 60 DEG C of thermostats and 42 DEG C of thermostats.
In a kind of embodiment, multichannel mechanism arm is 16 channel mechanical arms.
In a kind of embodiment, molecule is nucleic acid.
According to the automation molecular detector arrangement of above-described embodiment, by molecule extraction and detection fusion in an equipment,
Farthest avoid the falsehood of manual operation generation and the risk to personnel, environment.Meanwhile the device uses single detection
Encapsulation reagent device is detected as unit package reagent with each, and items selection is flexible, can place arbitrary project in one experiment
Reagent, and simultaneously extract and detect, substantially increase entry detection flexibility.
Description of the drawings
Fig. 1 is automation molecular detector arrangement schematic diagram；
Fig. 2 is that single detection encapsulates reagent device schematic diagram.
Specific implementation mode
Below by specific implementation mode combination attached drawing, invention is further described in detail.
In embodiments of the present invention, this example provides a kind of automation molecular detector arrangement, and the extraction of molecule and detection are melted
It closes into an equipment, structure chart is as shown in Figure 1, include portable inspectiont platform 1, thermostat, magnetic adsorption device and Duo Tong
Road mechanical arm 2.
Wherein, portable inspectiont platform 1 is equipped with multigroup sense channel 11, Ge Gejian that one is extracted and detected for molecule
It surveys the single detection that channel 11 is equipped with as unit of each detection and encapsulates reagent device 3, as shown in Fig. 2, the single inspection of this example
It includes conventional reagent preassembled unit 31 and special reagent preassembled unit 32, conventional reagent preassembled unit 31 to survey encapsulation reagent device 3
It is positioned on sense channel 11 with the splicing of special reagent preassembled unit 32, specifically, conventional reagent preassembled unit 31 includes dress
It is provided with several test tubes of all reagents of molecule extraction and augmentation detection process needs；Special reagent preassembled unit 32 includes
Test tube equipped with molecule extracting solution and internal standard mixed liquor and the test tube that enzyme is housed and is sealed with wax.
The special reagent preassembled unit 32 of this example is the environment stored with 31 maximum region of conventional reagent preassembled unit
Difference, e.g., the needs of special reagent preassembled unit 32 store in the environment of freezing, and conventional reagent preassembled unit 31 only need to be normal
Therefore the lower storage of temperature in practical applications, can pre-install special reagent preassembled unit 32 and conventional reagent single according to requiring
Member 31 stores respectively, and therefore, the conventional reagent preassembled unit 31 and special reagent preassembled unit 32 of this example are detachable splicing.
The quantity of several test tubes in the conventional reagent preassembled unit 31 of this example can depending on actually detected situation,
Specific encapsulation amount can also be depending on actually detected situation, wherein several test tubes in conventional reagent preassembled unit 31
In be at least empty test tube there are two test tube, the sky test tube for transfer liquid, storage waste liquid, isolation, heating or for extension etc.,
Other test tubes can fill cleaning solution, augmentation detection liquid etc. according to actual needs.
The thermostat of this example includes 60 DEG C of thermostats and 42 DEG C of thermostats, and thermostat is for providing different extractions
The temperature needed with amplification is placed on to thermostat variable below single detection encapsulation reagent device, e.g., when a certain single
When test tube in detection encapsulation reagent device needs 60 DEG C of isoperibols, then 60 DEG C of thermostats can be put by elevating mechanism
It is placed in below the test tube, 60 DEG C of isoperibols is provided to the test tube, after a period of time, then by elevating mechanism by 60 DEG C of constant temperature
Device rises to certain altitude, away from the test tube.In other embodiments, thermostat can be fixed under a certain test tube
Side, it would be desirable to which the liquid of warm bath moves in the test tube, again removes the liquid after warm bath after warm bath, in order under the test tube
Secondary warm bath uses.
The magnetic adsorption device of this example is used to provide the magnetic field environment that different extractions and amplification need, magnetic adsorption device variable
Ground is placed below single detection encapsulation reagent device, and the control process of magnetic adsorption device can also pass through such as thermostat
Elevating mechanism control magnetic adsorption device is positioned over below a certain test tube, or leaves the test tube.
After placing corresponding conventional reagent preassembled unit 31 and special reagent preassembled unit 32 on channel 11 to be detected, i.e.,
Controllable multiple channel mechanical arm 2 carries out pipetting, specifically, multiple channel mechanical arm 2 is corresponded with sense channel 11, it is mostly logical
Road mechanical arm 2 can encapsulate reagent to the single detection on corresponding sense channel 11 simultaneously under the control that molecule extracts and detects
Device 3 carries out pipetting, is finally completed extraction and the detection integration of molecule, it is preferred that the multiple channel mechanical arm 2 of this example is
16 channel mechanical arms can handle the pipetting of 16 detections simultaneously.
In addition, this example can also increase fluorescence reading device, each channel mechanical arm is read by fluorescence reading device and is detected
Fluorescence signal, carry out obtaining the corresponding testing result of the fluorescence signal, in order to operator watch.
It should be noted that the motion control of the multiple channel mechanical arm 2 of this example, the movement of thermostat and magnetic adsorption device
The modes such as control can be controlled by cylinder, motor controls are realized.
Such as, the movement of multiple channel mechanical arm 2 is driven by motor, and the pipetting of multiple channel mechanical arm 2 is driven by motor
Piston in cylinder is realized.The movement of thermostat, magnetic adsorption device and fluorescence reading device is driven by motor.
In the following, this example says the applicating example for automating molecular detector arrangement so that molecule is the extraction and detection of nucleic acid as an example
It is bright.
1) internal standard solution for being extracted 10ul in the test tube equipped with internal standard solution using big suction nozzle is added to and is carried equipped with 100ul nucleic acid
It takes in the test tube of liquid, nucleic acid extraction liquid and internal standard extracting solution is lashed into mixing, obtain nucleic acid/internal standard mixing liquid；
2) pretreated sample is extracted using big suction nozzle and be added in nucleic acid/internal standard mixing liquid, and lashed mixing and obtain sample
This mixing liquid；
3) sample mixing liquid is drawn using big suction nozzle, by it under 60 DEG C of thermostats first warm bath 10 minutes, then drop naturally
After magnetic suck, the waste liquid in the big suction nozzle is spat in waste liquid tank then by the big suction nozzle magnetic suck 2 minutes for temperature 10 minutes, and
Magnetic beads held is in the big suction nozzle；
4) after the big suction nozzle in utilizing 3) draws cleaning solution and lashes mixing in the big suction nozzle, magnetic suck 2 minutes, magnetic
Attached, the waste liquid in the big suction nozzle is spat in waste liquid tank, and magnetic beads held is in the big suction nozzle；
5) after the big suction nozzle in utilizing 4) draws cleaning solution and lashes mixing in the big suction nozzle, magnetic suck 2 minutes, magnetic
Attached, the waste liquid in the big suction nozzle is spat in waste liquid tank, and magnetic beads held is in the big suction nozzle；
6) the big suction nozzle in utilizing 5) draws augmentation detection liquid, and utilizes augmentation detection liquid in the concussion of the intracavitary of the big suction nozzle
Rinse magnetic bead, so that magnetic bead is spat into mineral oil pipe, and by the mineral oil pipe under 60 DEG C of thermostats warm bath 10 minutes；
7) liquid in mineral oil pipe is moved in enzyme pipe/paraffin pipe using small suction nozzle, carries out corresponding amplification processing.
Nucleic acid extraction and detection fusion in an equipment, are farthest avoided manual operation generation by the application
Falsehood and risk to personnel, environment.Meanwhile the device encapsulates reagent strip using single detection and is detected as unit package with each
Reagent, items selection is flexible, can place Arbitrary Term purpose reagent in one experiment, and extracts and detect simultaneously, greatly improves
The flexibility of entry detection, and using reagent strip as the container of extraction process, reduce liquid relief step, greatly reduce and carry
What is taken and detect takes, and the sample extraction of this example and detection take about 1 hour.
In addition, the present invention, in nucleic acid amplification and after experiment, consumptive material uses paraffin coating, can directly abandon, prevent
The effusion of external environmental reaction system and product.The automation molecular detector arrangement of the present invention has the characteristics that portable, can lead to
Vehicular accumulator cell powered operation is crossed, achievees the purpose that sample at any time, detect at any time.
Use above specific case is illustrated the present invention, is merely used to help understand the present invention, not limiting
The system present invention.For those skilled in the art, according to the thought of the present invention, can also make several simple
It deduces, deform or replaces.
Claims (7)
1. a kind of automation molecular detector arrangement, which is characterized in that including：
Portable inspectiont platform is equipped with and integrated multigroup sense channel is extracted and detected for molecule, on each sense channel
Reagent device is encapsulated equipped with the single detection as unit of each detection；
Thermostat, the temperature needed for providing different extractions and amplification, is placed on to the thermostat variable single
Below detection encapsulation reagent device；
Magnetic adsorption device, the magnetic field environment needed for providing different extractions and amplification, puts to the magnetic adsorption device variable
It sets below single detection encapsulation reagent device；
Multiple channel mechanical arm is corresponded with the sense channel, and the multiple channel mechanical arm is simultaneously on corresponding sense channel
Single detection encapsulation reagent device carry out pipetting, complete the extraction and detection of molecule；
Fluorescence reading device, for detecting the amplified production after the multiple channel mechanical arm extracts in liquid.
2. automation molecular detector arrangement as described in claim 1, which is characterized in that packet is set in the single detection encapsulation trial assembly
Conventional reagent preassembled unit and special reagent preassembled unit are included, the conventional reagent preassembled unit and special reagent preassembled unit are spelled
Ground connection is positioned on the sense channel；
If the conventional reagent preassembled unit includes equipped with all reagents for providing molecule extraction and augmentation detection process needs
Dry test tube；
The special reagent preassembled unit includes that the test tube of molecule extracting solution and internal standard mixed liquor is housed and enzyme is housed and is sealed with wax
Test tube.
3. automation molecular detector arrangement as claimed in claim 2, which is characterized in that the conventional reagent preassembled unit and institute
Special reagent preassembled unit is stated detachably to splice.
4. automation molecular detector arrangement as claimed in claim 2, which is characterized in that in the conventional reagent preassembled unit
There are two test tube it is at least empty test tube in several test tubes.
5. automation molecular detector arrangement as described in claim 1, which is characterized in that the thermostat includes 60 DEG C of constant temperature
Device and 42 DEG C of thermostats.
6. automation molecular detector arrangement as described in claim 1, which is characterized in that multichannel mechanism arm is 16 logical
Road mechanical arm.
7. automation molecular detector arrangement as described in claim 1, which is characterized in that the molecule is nucleic acid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810747165.3A CN108642052A (en) | 2018-07-09 | 2018-07-09 | A kind of automation molecular detector arrangement |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810747165.3A CN108642052A (en) | 2018-07-09 | 2018-07-09 | A kind of automation molecular detector arrangement |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110699241A (en) * | 2019-10-28 | 2020-01-17 | 军事科学院系统工程研究院卫勤保障技术研究所 | Automatic disinfection effect rapid evaluation device and method |
| CN112725162A (en) * | 2021-01-15 | 2021-04-30 | 山东大学 | Full-automatic microbial nucleic acid extraction and detection device and extraction and detection method |
| CN113174428A (en) * | 2021-01-15 | 2021-07-27 | 北京中科生仪科技有限公司 | Control method of PCR continuous reaction |
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| CN106916743A (en) * | 2017-03-19 | 2017-07-04 | 北京化工大学 | Integrated nucleic acid extraction and augmentation detection system |
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Patent Citations (5)
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| CN102954954A (en) * | 2012-09-19 | 2013-03-06 | 东南大学 | Magnetic separation-based multi-sample multi-site high-flux nucleic acid analysis system |
| WO2018014678A1 (en) * | 2016-07-19 | 2018-01-25 | 湖州高亿诺生物科技有限公司 | Automated microbial molecular assay device and method |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110699241A (en) * | 2019-10-28 | 2020-01-17 | 军事科学院系统工程研究院卫勤保障技术研究所 | Automatic disinfection effect rapid evaluation device and method |
| CN112725162A (en) * | 2021-01-15 | 2021-04-30 | 山东大学 | Full-automatic microbial nucleic acid extraction and detection device and extraction and detection method |
| CN113174428A (en) * | 2021-01-15 | 2021-07-27 | 北京中科生仪科技有限公司 | Control method of PCR continuous reaction |
| CN113174428B (en) * | 2021-01-15 | 2021-12-31 | 北京中科生仪科技有限公司 | Control method of PCR continuous reaction |
| CN112725162B (en) * | 2021-01-15 | 2022-03-01 | 山东大学 | Full-automatic microbial nucleic acid extraction and detection device and extraction and detection method |
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