CN108641951A - A kind of sterile drying equipment of plant cell cultures - Google Patents
A kind of sterile drying equipment of plant cell cultures Download PDFInfo
- Publication number
- CN108641951A CN108641951A CN201810398407.2A CN201810398407A CN108641951A CN 108641951 A CN108641951 A CN 108641951A CN 201810398407 A CN201810398407 A CN 201810398407A CN 108641951 A CN108641951 A CN 108641951A
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- CN
- China
- Prior art keywords
- air
- culture
- babinet
- drying
- laying rack
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/14—Drying
Abstract
The present invention provides a kind of sterile drying equipments of culture, it includes babinet, sterilizing filter, air -return duct, moist chamber, wind turbine, laying rack and airing net, in box house, it sets gradually as the moist chamber, sterilizing filter and laying rack, sterilizing filter is arranged close to the side of babinet, it is moist chamber between sterilizing filter and babinet, sterilizing filter completely separates moist chamber and the laying rack, upper end or following or side in babinet are equipped with air -return duct, air -return duct is vertically arranged with the sterilizing filter, air -return duct is connected to the other side of moist chamber and babinet, wind turbine is arranged in institute's air -return duct or in moist chamber, the airing net is arranged on laying rack.The sterile apparatus for drying of culture of the present invention, it can be used through the wind after sterilizing filter degerming, it is opposite to and air-dried article is needed to be air-dried on laying rack, and it dehumidifies, effectively prevent the growth of microorganism, guarantee is air cured not being contaminated for article, and passes through air -return duct, circulated air is formed, air-dried speed is accelerated.
Description
Technical field
The invention belongs to biotechnology production equipment technologies, and in particular to a kind of sterile apparatus for drying of culture.
Background technology
The bioreactor of culture plant cell, tissue and organ has arrived the stage of industrialized production at present.Example
Such as, serfin is produced with airlift bioreactor culture Catharanthus Roseus Cell, South Korea S.H.Son et al. carries out yew cell
Reactor large-scale culture, using the airlift reactor from 100L to 500L, Kee-Yoeup Paek are using spherical bubble type
Reactor carries out large-scale culture to ginseng adventitious root and obtains the ginsenoside with very strong pharmacological activity, obtains compared with Seedling height
The ginseng adventitious root etc. of amount.Meiyan Inst. of Biological Engineering, Meixian County produces yew cell using gas circulating type bioreactor
It is also up to the level of 500L, the cell culture fluid obtained for reactor can not be utilized directly, but need to use
Drying equipment is dried, during the drying process it is also noted that protecting the bioactivity of cell culture, but for for training
The equipment for supporting the drying of object yet there are no.
Existing drying equipment is easy microbiological contamination, since the drying temperature of use is not in culture carries out drying course
It is very high, cause the used time longer, it is exceeded to eventually lead to microorganism in culture, and since culture moisture is larger, causes to air-dry and sets
Standby interior humidity is larger, and humidity is easy to cause greatly mould growth quasi-microorganism, therefore very long in air drying process, microbial growth
Time is long, further increases the possibility of pollution, results even in the rotten of culture, causes serious financial consequences.
Invention content
In order to solve the above problem in the prior art, the present invention provides a kind of sterile apparatus for drying of culture, the equipment
Can effective degerming, air-dried, for plant cell it is sterile air-dried when, microorganism subtracts significantly in obtained culture after air-drying
It is few.
To achieve the above object, the present invention use technical solution below for：
A kind of sterile apparatus for drying of culture comprising babinet, air -return duct, moist chamber, wind turbine, is put sterilizing filter
Frame and airing net are set gradually in the box house as the moist chamber, sterilizing filter and laying rack, the sterile mistake
Filter is arranged close to the side of babinet, is the moist chamber, the aseptic filtration between the sterilizing filter and the babinet
Device completely separates the moist chamber and the laying rack, and the upper end or following or side in the babinet are equipped with air -return duct, institute
Air -return duct to be stated to be vertically arranged with the sterilizing filter, the air -return duct is connected to the other side of the moist chamber and the babinet,
In institute's air -return duct or in moist chamber, the airing net is arranged on laying rack for the wind turbine setting.Culture as described above
Sterile apparatus for drying, it is preferable that dehumidification device is equipped in the moist chamber.
The sterile apparatus for drying of culture as described above, it is preferable that the indoor bottom of dehumidifying is equipped with drainage, described
Recovery tube is arranged below in drainage, and the other end of the recovery tube leads to the outside of the babinet.
The sterile apparatus for drying of culture as described above, it is preferable that the laying rack is equipped with multiple, movable removal case
Body, each laying rack are set as multilayer, and every layer is arranged for the airing net.
The sterile apparatus for drying of culture as described above, it is preferable that the laying rack is set as multilayer, and every layer of top is equipped with
Airing net, lower section are equipped with drawing box.
The sterile apparatus for drying of culture as described above, it is preferable that the sterilizing filter or its filter core are detachably to set
It sets.
The sterile apparatus for drying of culture as described above, it is preferable that the sterile apparatus for drying of culture further includes air
Filter, the air filter are set to the other side of the babinet relative to sterilizing filter.
The sterile apparatus for drying of culture as described above, it is preferable that the wind turbine is fan or hot air apparatus.
The sterile apparatus for drying of culture as described above, it is preferable that the sterilizing filter or air filter carry
0.22 Mm filter film.
The sterile apparatus for drying of culture as described above, it is preferable that the babinet is set as door, the door face pendulum on one side
Put frame setting.
The sterile apparatus for drying of culture as described above, it is preferable that the lower end of the laying rack is equipped with export-oriented wheel or pulley,
The card slot of fixed laying rack is equipped in the babinet.
The beneficial effects of the present invention are：
The sterile apparatus for drying of culture of the present invention, can be used through the wind after sterilizing filter degerming, is opposite to and puts
It needs air-dried article to be air-dried on frame, and dehumidifies, effectively prevent the growth of microorganism, guarantee is air cured article not
It is contaminated, and by air -return duct, form circulated air, accelerate air-dried speed.
It can quickly remove using the equipment moisture on culture for saussurea involucrata cell culture, and culture
Microbiological indicator be greatly reduced, detect the product micro organism quantity of common drying box drying by testing and mostly can not
The index of meter, and the Saussurea involucrata culture total plate count for using the sterile apparatus for drying of the present invention air-dried mostly 10,000 hereinafter, accord with completely
Close the standard requirement of culture.
Description of the drawings
Fig. 1 is the structural schematic diagram of a preferred sterile apparatus for drying of culture of the invention.
Fig. 2 is the structural schematic diagram of a preferred laying rack of the invention.
Reference sign
1, babinet；2, sterilizing filter；3, air -return duct；4, moist chamber；5, hurricane drier；6, dehumidification device；7, laying rack；8、
Airing net；9, air filter.
Specific implementation mode
Following embodiment should not be construed as limiting the invention for further illustrating the present invention.Without departing substantially from this
Under the premise of spirit and essence, modification or replacement made for the present invention belong to scope of the invention.
Unless otherwise specified, the conventional means that technological means used in embodiment is well known to those skilled in the art.
Embodiment 1
A kind of sterile apparatus for drying of culture, as shown in Figure 1, including babinet 1, sterilizing filter 2, air -return duct 3, moist chamber
4, wind turbine, laying rack and airing net are set gradually in the inside of babinet 1 as moist chamber 4, sterilizing filter 2 and laying rack, nothing
Bacterium filter 2 is arranged close to the side of babinet 1, is moist chamber 4, laying rack position between sterilizing filter 2 and babinet 1
For hurricane drier 5, sterilizing filter 2 completely separates moist chamber 4 with laying rack, ensures through the wind of hurricane drier to be to pass through nothing completely
Bacterium filter is filtrated air, and the upper end in babinet 1 is equipped with air -return duct 3, and air -return duct 3 is vertically arranged with sterilizing filter 2,
Air -return duct 3 is connected to moist chamber 4 and hurricane drier 5 is the other side of babinet, and wind turbine is arranged in institute's air -return duct；The wind for making wind turbine blow out
The filtrated air after degerming is become by sterilizing filter 2 by the entrance of air -return duct 3 moist chamber 4, into hurricane drier 5, can be incited somebody to action
It is placed in the objects such as article or the culture of laying rack to be air-dried, wind enters back into air -return duct, can once carry out cycle and air-dry.It puts
Airing net is provided on frame, laying rack is provided with multilayer, and every layer is separated by airing net, and article is placed in the enterprising sector-style of airing net
It is dry.
It is equipped with dehumidification device 6 in moist chamber, dehumidifies to moisture, air-dried indoor air humidity is substantially reduced, is carried
The operating efficiency of high bellows.Dehumidification device is made of compressor, heat exchanger, fan, water container, casing and controller, is filled with water
Device can be set to the outside of babinet, and remaining component is set to inside moist chamber, and dehumidification device also provides for the sterile apparatus for drying of culture
Dry wind.
Further, be equipped with drainage in the bottom of dehumidification device, drainage is arranged below recovery tube, recovery tube it is another
End leads to outside babinet, can will be collected into moisture and be discharged to except equipment.
It is provided in the laying rack of hurricane drier multiple, laying rack is not fixed setting, is independent movable removal babinet, such as
Shown in Fig. 2, laying rack 7 is arranged for multilayer, and every layer is set as airing net 8, that is, is used for drying article, and airing net 8 is porose nylon
Wire side or other perforated spacers of tool.The lower end of laying rack can also be equipped with export-oriented wheel or pulley moves convenient for laying rack.When
When equipped with pulley, it is equipped with card slot in hurricane drier, laying rack is stablized and is fixed.
When drying has the bigger material of moisture, the moisture of the material of last layer drips to next layer of object in order to prevent
Material, is additionally provided with drawing box, drawing box is mobilizable, can be extracted out at any time, and the setting of drawing box is every below airing net
The lower section of layer airing net is equipped with frame, and drawing box can be fastened on frame, when not used, removes drawing box, airing net up and down
Fang Junwei skies, facilitate air circulation.In order to facilitate sterilizing filter is replaced, sterilizing filter or its filter core are set as removable
Setting is unloaded, engaging setting can be set as.Sterilizing filter is 0.22 Mm filter film, can effectively cross air filtering, remove microorganism, shape
At sterile wind.
In order to further reach gnotobasis, as shown in Figure 1, in the other side of hurricane drier, it is another far from sterilizing filter
One end is additionally provided with air filter 9, and air filter 9 is used to remove the microorganism in air, and air filter can be set as carrying
0.22 Mm filter film or filter core.The opening of the other end of ventilating duct is then the other end for being connected to air filter 9, and air passes through
After air filter 9, ventilating duct is entered back into.
It removes and moves into order to facilitate laying rack, door is equipped in the side of the babinet of face laying rack.
When cell culture using above equipment air-dry, the moisture on culture can be quickly removed, and
The microbiological indicator of culture is greatly reduced.It is detected by testing, when the product dried with common drying box, such as saussurea involucrata
Culture, total plate count is in the index that can not mostly count, and the Saussurea involucrata culture for using the sterile apparatus for drying to air-dry, bacterium colony are total
Number is 10,000 hereinafter, complying fully with the standard requirement of culture.
Embodiment 2
The present embodiment is that air -return duct can be set as under babinet or side on the basis of embodiment 1.Wind turbine is arranged
In moist chamber, wind turbine can be that fan is arranged, and wind is made directly by sterilizing filter, to be air-dried to air-drying indoor article.Root
According to needs, air -return duct can be set as one or several parallel circular pipe, and wind is made to be recycled.
When need certain temperature it is air-dried when, wind turbine can be set as the wind turbine with hot air apparatus, by hot wind into sector-style
It is dry, air-dried speed can be accelerated, air-dried suitable for the article not high to temperature requirement.
Claims (10)
1. a kind of sterile apparatus for drying of culture, which is characterized in that it include babinet, sterilizing filter, air -return duct, moist chamber,
Wind turbine, laying rack and airing net are disposed with the moist chamber, sterilizing filter and laying rack, institute in the box house
It states sterilizing filter close to the side of babinet to be arranged, is the moist chamber between the sterilizing filter and the babinet, it is described
Sterilizing filter completely separates the moist chamber and the laying rack, and the upper end or following or side in the babinet are equipped with back
Air duct, the air -return duct are vertically arranged with the sterilizing filter, and the air -return duct is connected to the moist chamber and the babinet
The other side, in institute's air -return duct or in moist chamber, the airing net is arranged on laying rack for the wind turbine setting.
2. the sterile apparatus for drying of culture as described in claim 1, which is characterized in that be equipped with dehumidifying dress in the moist chamber
It sets.
3. the sterile apparatus for drying of culture as described in claim 1, which is characterized in that the indoor bottom of dehumidifying is equipped with row
Recovery tube is arranged below in water channel, the drainage, and the other end of the recovery tube leads to the outside of the babinet.
4. the sterile apparatus for drying of culture as described in claim 1, which is characterized in that the laying rack be equipped with it is multiple, can live
Dynamic to remove babinet, each laying rack is set as multilayer, and every layer is arranged for the airing net.
5. the sterile apparatus for drying of culture as described in claim 1, which is characterized in that the laying rack is set as multilayer, every layer
Top be equipped with airing net, lower section be equipped with drawing box.
6. the sterile apparatus for drying of culture as described in claim 1, which is characterized in that the sterilizing filter or its filter core are
Detachable setting.
7. the sterile apparatus for drying of culture as described in claim 1, which is characterized in that the sterile apparatus for drying of culture is also
Including air filter, the air filter is set to the other side of the babinet, the sterile mistake relative to sterilizing filter
Filter or air filter carry 0.22 Mm filter film.
8. the sterile apparatus for drying of culture as described in claim 1, which is characterized in that the wind turbine is that fan or hot wind fill
It sets.
9. the sterile apparatus for drying of culture as described in claim 1, which is characterized in that the babinet is set as door, institute on one side
State a face laying rack setting.
10. the sterile apparatus for drying of culture as described in claim 1, which is characterized in that the lower end of the laying rack is equipped with outer
To wheel or pulley, the card slot of fixed laying rack is equipped in the babinet.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810398407.2A CN108641951A (en) | 2018-04-28 | 2018-04-28 | A kind of sterile drying equipment of plant cell cultures |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810398407.2A CN108641951A (en) | 2018-04-28 | 2018-04-28 | A kind of sterile drying equipment of plant cell cultures |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108641951A true CN108641951A (en) | 2018-10-12 |
Family
ID=63748165
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810398407.2A Pending CN108641951A (en) | 2018-04-28 | 2018-04-28 | A kind of sterile drying equipment of plant cell cultures |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108641951A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112833643A (en) * | 2021-01-21 | 2021-05-25 | 梁庆开 | Electric heat blast air drying cabinet for cell culture |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH05137564A (en) * | 1991-11-21 | 1993-06-01 | Nitto Denko Corp | Method for multistage type culture and its apparatus |
| CN204154063U (en) * | 2014-03-27 | 2015-02-11 | 张建岗 | A kind of drying equipment |
| CN205082633U (en) * | 2015-07-30 | 2016-03-16 | 福建信龙农产品开发有限公司 | White fungus sunning frame |
-
2018
- 2018-04-28 CN CN201810398407.2A patent/CN108641951A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH05137564A (en) * | 1991-11-21 | 1993-06-01 | Nitto Denko Corp | Method for multistage type culture and its apparatus |
| CN204154063U (en) * | 2014-03-27 | 2015-02-11 | 张建岗 | A kind of drying equipment |
| CN205082633U (en) * | 2015-07-30 | 2016-03-16 | 福建信龙农产品开发有限公司 | White fungus sunning frame |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112833643A (en) * | 2021-01-21 | 2021-05-25 | 梁庆开 | Electric heat blast air drying cabinet for cell culture |
| CN112833643B (en) * | 2021-01-21 | 2022-08-26 | 北京中城汇联科技发展有限公司青岛研发中心 | Electric heat blast air drying cabinet for cell culture |
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Address after: 116000 Changdianbao Community, Pulandian Economic Development Zone, Dalian, Liaoning Province Applicant after: Puruikang Biotechnology Co., Ltd., Dalian Address before: 116023 No. 32 Lixian Street, Qixianling, Dalian High-tech Industrial Park, Liaoning Province Applicant before: Puruikang Biotechnology Co., Ltd., Dalian |