CN108641932B - Method for sticking drug sensitive paper sheet - Google Patents
Method for sticking drug sensitive paper sheet Download PDFInfo
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- CN108641932B CN108641932B CN201810382592.6A CN201810382592A CN108641932B CN 108641932 B CN108641932 B CN 108641932B CN 201810382592 A CN201810382592 A CN 201810382592A CN 108641932 B CN108641932 B CN 108641932B
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- paper sheet
- culture dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/18—Testing for antimicrobial activity of a material
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- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
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- Toxicology (AREA)
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Abstract
The invention relates to a method for sticking a drug sensitive paper sheet, which comprises the following steps: the first step is as follows: moving the paper support column to the outside of the two square sliding blocks, then reversely buckling the culture dish onto the positioning ruler, buckling the two square sliding blocks, then respectively moving the two square sliding blocks to the two sides until the distance between the two square sliding blocks is maximum, then moving the whole culture dish and the square sliding blocks together to the middle part of the positioning ruler, wherein the scales of the square sliding blocks on the two sides are the same, the middle point of the positioning ruler is the center of a circle of the culture dish, and then screwing the fixing knob to lock the position; the second step is that: the number of the drug sensitive paper sheets to be adhered and the shape of the paper sheets to be adhered in the culture dish are determined, then one drug sensitive paper sheet is placed on the paper sheet supporting column, the positioning ruler and the supporting ruler are opened at an angle along the hinge, and the paper sheet supporting column is moved to a required position.
Description
Technical Field
The invention relates to a method for sticking a drug sensitive paper sheet.
Background
At present, in a medical microorganism laboratory, the most important work is the separation and the verification of microorganisms, and drug sensitivity tests are used for measuring the drug sensitivity of bacteria so as to guide the clinical more accurate and effective treatment by using drugs. The conventional methods include a drug sensitive paper sheet method (or a paper sheet method), a dilution method, an E-test (gradient) method, and the like. The paper sheet method is a common method for general manual operation because of the advantages of convenient operation, less consumption of materials and the like.
When the sheet application is performed manually, there are often operational problems that result in improper sheet application positions or an insufficiently uniform sheet distribution and may lead to errors in the interpretation of the results. However, the currently available drug sensitive paper sheet dispensers are generally fixed in size (e.g. fixed in diameter of 6cm or 10cm) and in mode (e.g. 6 paper sheets or 8 paper sheets are stuck at one time), and are not suitable for sticking flat plates with different sizes or a plurality of types of paper sheets. And often are of the face-on type, which can easily cause contamination of the flat sheet or paper dispenser.
Disclosure of Invention
The invention aims to provide a method for pasting a drug sensitive paper sheet, which solves the problem that the manual pasting position in the prior art is not stable and uniform enough.
In order to achieve the above purpose, the invention adopts the technical scheme that: a method for pasting a drug sensitive paper sheet comprises the following steps:
the first step is as follows: moving the paper support column to the outside of the two square sliding blocks, then reversely buckling the culture dish onto the positioning ruler, buckling the two square sliding blocks, then respectively moving the two square sliding blocks to the two sides until the distance between the two square sliding blocks is maximum, then moving the whole culture dish and the square sliding blocks together to the middle part of the positioning ruler, wherein the scales of the square sliding blocks on the two sides are the same, the middle point of the positioning ruler is the center of a circle of the culture dish, and then screwing the fixing knob to lock the position;
the second step is that: determining the number of the drug sensitive paper sheets to be adhered and the shape of the paper sheets to be adhered in a culture dish, then placing one drug sensitive paper sheet on a paper sheet supporting column, opening a positioning ruler and a supporting ruler at an angle along a hinge, and moving the paper sheet supporting column to a required position;
the third step: pressing the positioning ruler and the supporting ruler, pressing the drug sensitive paper sheets on the culture dish, reopening the positioning ruler and the supporting ruler, replacing new drug sensitive paper sheets on the paper sheet supporting columns, rotating the culture dish to a required angle, pressing again and sticking a second piece of drug sensitive paper sheets;
repeating the above process until the paper sheet is completely pasted.
Furthermore, in the third step, the culture dish is marked before the rotation angle.
Preferably, in the second step, the positioning rule and the support rule are opened along the hinge by an angle of less than 15 °.
The invention has the technical effects that:
(1) the paper sheet is fixed by the tool when being pasted, and the position is uniform and more stable than the common manual pasting position.
(2) The angle can be freely rotated during pasting, the distance of the circle center can be freely selected, the method is not limited to a fixed mode (for example, the existing part of the drug sensitive paper sheet distributor is fixed with 6 holes or 8 holes), and the position selection is flexible.
(3) The culture dish has wide application range, the diameter of the culture dish can be used without exceeding the full length of the positioning ruler, and the culture dish with specific specification is not needed.
(4) When the paper sheets are pasted, the culture dish is not in contact with objects outside the paper sheets, and pollution is avoided.
(5) Compared with other pasting devices and methods, the culture dish is in an inverted buckling state in the whole pasting process, an opening is not required to be upwards, and the pollution probability of external bacteria sedimentation can be reduced.
Drawings
FIG. 1 is a front view of the apparatus of the present invention;
FIG. 2 is a side view of the apparatus of the present invention;
FIG. 3 is a front view of the device of the present invention in a practical application state;
FIG. 4 is a side view of the device of the present invention in a practical use;
FIG. 5 is a front view of another state of the device of the present invention in practical use, namely, the state where a drug sensitive paper sheet is placed on the paper sheet support column;
FIG. 6 is a side view of another state of practical application of the device of the present invention;
FIG. 7 is a front view of the culture dish in a labeled state;
in the figure, a is the solid culture medium at the bottom layer of the culture dish and is the place where the bacteria actually grow; b is the mark on the culture dish.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The specific method of the invention comprises the following steps:
the first step is as follows: move scraps of paper support column 8 outside two square slide blocks 2 earlier, then with the culture dish back-off to the locating ruler 1 on, withhold two square slide blocks 2, then move two square slide blocks 2 to both sides respectively, until the distance is the biggest between two square slide blocks 2, then move whole culture dish and square slide block 2 to locating ruler 1 middle part together, and the shown scale of square slide block 2 in both sides is the same, the midpoint of locating ruler 1 is the centre of a circle of culture dish this moment, then tighten fixed knob (6), locking position.
The second step is that: the number of drug sensitive paper pieces to be stuck and the shape to be stuck in the culture dish are determined, then one drug sensitive paper piece is put on the paper piece supporting column 8, the positioning rule 1 and the supporting rule 4 are slightly opened (the opening angle is less than 15 degrees) along the hinge 7, and the paper piece supporting column 8 is moved to a required position.
The third step: the positioning ruler 1 and the supporting ruler 4 are lightly pressed, and at the moment, the drug sensitive paper sheets are pressed on the culture dish. And (3) reopening the two rulers of the positioning ruler 1 and the supporting ruler 4, replacing a new drug sensitive paper sheet on the paper sheet supporting column 8, rotating the culture dish to a required angle, and pressing again to paste a second drug sensitive paper sheet. Repeating the above process until the paper sheet is completely pasted.
Furthermore, in the third step, the rotation angle may be marked on the culture dish.
The invention has the advantages that:
(1) the paper sheet is fixed by the tool when being pasted, and the position is uniform and more stable than the common manual pasting position.
(2) The angle can be freely rotated during pasting, the distance of the circle center can be freely selected, the method is not limited to a fixed mode (for example, the existing part of the drug sensitive paper sheet distributor is fixed with 6 holes or 8 holes), and the position selection is flexible.
(3) The culture dish has wide application range, the diameter of the culture dish can be used without exceeding the full length of the positioning ruler 1, and the culture dish with specific specification is not needed.
(4) When the paper sheets are pasted, the culture dish is not in contact with objects outside the paper sheets, and pollution is avoided.
(5) Compared with other pasting devices and methods, the culture dish is in an inverted buckling state in the whole pasting process, an opening is not required to be upwards, and the pollution probability of external bacteria sedimentation can be reduced.
Referring to the attached drawings, the drug sensitive paper sheet sticking device comprises a positioning rule 1 and a supporting rule 4, wherein the positioning rule 1 is hinged with one end of the supporting rule 4 through a hinge 7, and the other end of the positioning rule is freely arranged;
the positioning ruler 1 is provided with a hollowed-out chute 3 along the length direction, the hollowed-out chute 3 comprises a first guide rail groove arranged on the front surface of the positioning ruler 1 and a second guide rail groove arranged on the side surface of the positioning ruler 1, two square sliders 2 are arranged in the hollowed-out chute 3, the upper ends of the square sliders 2 are arranged in the first guide rail groove, the lower ends of the square sliders 2 are arranged in the second guide rail groove, the square sliders 2 can freely slide along the arrangement direction of the hollowed-out chute 3, a protruding block 5 is arranged at the upper ends of the square sliders 2, a fixing knob 6 is arranged at the lower ends of the square sliders 2, the protruding block 5 is arranged outside the first guide rail groove, the fixing knob 6 is arranged outside the second guide rail groove, and the fixing knob 6 limits the free sliding of the square sliders 2 through rotating action;
the zero scale on the positioning ruler 1 is arranged in the middle of the positioning ruler, and the positioning ruler 1 extends from the middle to two sides to gradually increase in scale value;
a third guide rail groove is formed in the front face of the supporting ruler 4, a paper sheet supporting column 8 is arranged in the third guide rail groove, the upper end of the paper sheet supporting column 8 extends into the first guide rail groove, and the paper sheet supporting column 8 can freely slide along the third guide rail groove.
Further, the cross section of the protruding block 5 is triangular or V-shaped.
Still further, a movable handle 9 is arranged between the fixed knob 6 and the square sliding block 2.
Preferably, the paper sheet supporting column 8 can stretch up and down.
With the help of the attached drawings, the middle of the positioning ruler 1 is provided with the hollowed-out sliding groove 3, the square sliding block 2 can slide in the hollowed-out sliding groove 3 and is locked by the fixing knob 6, the front surface and the side surface of the positioning ruler 1 are respectively provided with the guide rail grooves (the first guide rail groove and the second guide rail groove), the square sliding block 2 can conveniently slide freely in the guide rail grooves, and the square sliding block 2 plays a role in positioning the culture dish. The support ruler 4 is also provided with a slide groove structure (third guide rail groove) in the middle thereof, in which the sheet support post 8 moves. One end between the positioning rule 1 and the supporting rule 4 is hinged by a hinge 7, and the other end can be freely opened and closed. The zero scale of the rule 1 increases in value as it extends to both sides at the middle of the rule, i.e. a type of rule of 10, 9, 8 … … 2, 1, 0, 1, 2 … … 9, 10. The upper end of the square sliding block 2 is provided with a triangular or V-shaped protruding block 5 which is used for adjusting the position of the culture dish, determining the center of the culture dish and fixing the culture dish when the culture dish is reversely buckled on the positioning ruler 1. The position of scraps of paper support column 8 can be adjusted according to the demand to the culture dish back-off on location chi 1 and fixed back.
Preferably, the paper sheet supporting column 8 can be stretched up and down. The reason is that the thickness of part of the culture medium is different, the paper support columns 8 can be properly heightened if being lower, and the force is required to be light when the paper support columns 8 are higher, so that the bottom is not needed, and the culture dish is prevented from being crushed.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof.
Claims (3)
1. A method for pasting a drug sensitive paper sheet is characterized by comprising the following steps:
the first step is as follows: firstly, moving a paper support column (8) to the outside of two square sliding blocks (2), then reversely buckling a culture dish onto a positioning ruler (1), buckling the two square sliding blocks (2), then respectively moving the two square sliding blocks (2) to two sides until the distance between the two square sliding blocks (2) is maximum, then moving the whole culture dish and the square sliding blocks (2) to the middle part of the positioning ruler (1) together, wherein scales shown by the square sliding blocks (2) at the two sides are the same, the middle point of the positioning ruler (1) is the circle center of the culture dish at the moment, then screwing a fixing knob (6), and locking the position;
the second step is that: determining the number of the drug sensitive paper sheets to be adhered and the shape of the paper sheets to be adhered in a culture dish, then placing one drug sensitive paper sheet on a paper sheet supporting column (8), opening the positioning ruler (1) and the supporting ruler (4) at an angle along the hinge (7), and moving the paper sheet supporting column (8) to a required position;
the third step: pressing the positioning ruler (1) and the supporting ruler (4), pressing the drug sensitive paper sheets on the culture dish, reopening the positioning ruler (1) and the supporting ruler (4), replacing new drug sensitive paper sheets on the paper sheet supporting columns (8), rotating the culture dish to a required angle, pressing again and sticking a second piece of drug sensitive paper sheets;
repeating the above process until the paper sheet is completely pasted.
2. The method for sticking a drug sensitive paper sheet according to claim 1, wherein: in the third step, the culture dish is marked before the rotation angle.
3. The method for sticking a drug sensitive paper sheet according to claim 1, wherein: in the second step, the angle of opening the positioning ruler (1) and the supporting ruler (4) along the hinge (7) is less than 15 degrees.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810382592.6A CN108641932B (en) | 2018-04-26 | 2018-04-26 | Method for sticking drug sensitive paper sheet |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810382592.6A CN108641932B (en) | 2018-04-26 | 2018-04-26 | Method for sticking drug sensitive paper sheet |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108641932A CN108641932A (en) | 2018-10-12 |
| CN108641932B true CN108641932B (en) | 2020-09-11 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810382592.6A Active CN108641932B (en) | 2018-04-26 | 2018-04-26 | Method for sticking drug sensitive paper sheet |
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| CN (1) | CN108641932B (en) |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108642124B (en) * | 2018-04-26 | 2021-10-19 | 合肥迪安医学检验所有限公司 | Quick scraps of paper paster device of medicine |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| SE442021B (en) * | 1984-05-16 | 1985-11-25 | Biodisk Ab | SET AND DEVICE FOR APPLICATION OF OBJECTS LIKE ANTIBIOTIC COATS ON A RECEIVING DOWNTOWN SURFACE |
| CN101561238B (en) * | 2009-06-02 | 2010-12-08 | 西南铝业（集团）有限责任公司 | Measuring tool for measuring aluminum alloy ring and measuring method |
| CN205398610U (en) * | 2016-03-11 | 2016-07-27 | 河南金泰生物技术股份有限公司 | Quick scraps of paper distributor of electronic medicine |
| CN107344364B (en) * | 2017-09-04 | 2023-12-19 | 武汉申安智能系统股份有限公司 | Tool clamp for batch orderly transfer of culture dishes |
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Address after: 230000, building 9, Xing Lu tech Industrial Park, Luyang Industrial Zone, Hefei, Anhui Applicant after: Hefei Dean Medical Laboratory Co.,Ltd. Address before: 230000, building 9, Xing Lu tech Industrial Park, Luyang Industrial Zone, Hefei, Anhui Applicant before: HEFEI DIAN MEDICAL LABORATORY Co.,Ltd. |
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