CN108640373B - Method for promoting rapid separation of algae liquid in microalgae wastewater treatment by using calamus water extract - Google Patents
Method for promoting rapid separation of algae liquid in microalgae wastewater treatment by using calamus water extract Download PDFInfo
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 51
- 235000011996 Calamus deerratus Nutrition 0.000 title claims abstract description 39
- 238000000034 method Methods 0.000 title claims abstract description 25
- 241000195493 Cryptophyta Species 0.000 title claims abstract description 23
- 238000000926 separation method Methods 0.000 title claims abstract description 21
- 239000007788 liquid Substances 0.000 title claims abstract description 20
- 238000004065 wastewater treatment Methods 0.000 title claims abstract description 18
- 230000001737 promoting effect Effects 0.000 title claims abstract description 6
- 240000008167 Calamus deerratus Species 0.000 title abstract 2
- 239000002351 wastewater Substances 0.000 claims abstract description 27
- 238000001914 filtration Methods 0.000 claims abstract description 12
- 238000002791 soaking Methods 0.000 claims abstract description 9
- 230000003311 flocculating effect Effects 0.000 claims abstract description 8
- 230000015572 biosynthetic process Effects 0.000 claims abstract description 6
- 238000005286 illumination Methods 0.000 claims abstract description 6
- 238000012258 culturing Methods 0.000 claims abstract description 5
- 244000205574 Acorus calamus Species 0.000 claims description 41
- 244000249201 Scenedesmus obliquus Species 0.000 claims description 22
- 235000007122 Scenedesmus obliquus Nutrition 0.000 claims description 22
- 239000008367 deionised water Substances 0.000 claims description 10
- 229910021641 deionized water Inorganic materials 0.000 claims description 10
- 239000010865 sewage Substances 0.000 claims description 6
- 239000000470 constituent Substances 0.000 claims description 3
- 238000005520 cutting process Methods 0.000 claims description 3
- 239000012982 microporous membrane Substances 0.000 claims 1
- 238000005189 flocculation Methods 0.000 abstract description 14
- 238000004062 sedimentation Methods 0.000 abstract description 11
- 230000000694 effects Effects 0.000 abstract description 9
- 238000005516 engineering process Methods 0.000 description 6
- 230000016615 flocculation Effects 0.000 description 5
- 230000005764 inhibitory process Effects 0.000 description 5
- 235000006480 Acorus calamus Nutrition 0.000 description 4
- 238000005265 energy consumption Methods 0.000 description 3
- 238000003306 harvesting Methods 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 239000002131 composite material Substances 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- KMUONIBRACKNSN-UHFFFAOYSA-N potassium dichromate Chemical compound [K+].[K+].[O-][Cr](=O)(=O)O[Cr]([O-])(=O)=O KMUONIBRACKNSN-UHFFFAOYSA-N 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000002798 spectrophotometry method Methods 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 1
- WYWFMUBFNXLFJK-UHFFFAOYSA-N [Mo].[Sb] Chemical compound [Mo].[Sb] WYWFMUBFNXLFJK-UHFFFAOYSA-N 0.000 description 1
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 238000005188 flotation Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000001471 micro-filtration Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 238000005375 photometry Methods 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- USHAGKDGDHPEEY-UHFFFAOYSA-L potassium persulfate Chemical compound [K+].[K+].[O-]S(=O)(=O)OOS([O-])(=O)=O USHAGKDGDHPEEY-UHFFFAOYSA-L 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000002352 surface water Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F9/00—Multistage treatment of water, waste water or sewage
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/001—Processes for the treatment of water whereby the filtration technique is of importance
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/30—Treatment of water, waste water, or sewage by irradiation
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F2001/007—Processes including a sedimentation step
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- Life Sciences & Earth Sciences (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Separation Of Suspended Particles By Flocculating Agents (AREA)
Abstract
The invention provides a method for promoting rapid separation of algae liquid in microalgae wastewater treatment by using calamus water extract, which comprises the following steps: (1) soaking rhizoma Acori Calami in water for 25-35 days, and filtering to obtain rhizoma Acori Calami water extractive solution; (2) adding rhizoma Acori Calami water extractive solution into wastewater containing microalgae, culturing at 29 + -0.5 deg.C under illumination intensity of 3500lx and light-dark ratio of 14h:10h to promote formation of microalgae flocculating body and allow the microalgae flocculating body to settle. The method has the advantages of strong microalgae self-flocculation sedimentation effect, capability of promoting the growth of microalgae, short self-flocculation time, quick sedimentation and remarkable effect.
Description
Technical Field
The invention relates to a method for accelerating the separation of algae liquid in wastewater treatment, in particular to a method for applying calamus water extract to scenedesmus obliquus treatment wastewater to promote the rapid separation of algae liquid.
Background
The discharge standard of the treated sewage is still far higher than the water quality standard of surface water class V even if the treated sewage reaches the first-level A, and the discharged wastewater needs to be subjected to advanced treatment for improving the ecological environment and improving the reutilization of water resources and wastes. A large number of researches show that the microalgae has a wide prospect in treating wastewater and recycling the pollutants in the wastewater.
In recent years, the technology for treating wastewater by using microalgae is always stopped, and is mainly limited by algae liquid separation in the wastewater treatment process by algae, wherein the energy consumption of algae liquid separation accounts for 20-30% of the total cost of wastewater treatment by algae [1], and the problem that the algae liquid separation is the key for wastewater treatment by algae is solved. The algae liquid separation technology mainly comprises [2-3 ]: inorganic flocculation, composite electrolyte flocculation, composite flocculation, centrifugation technology, filtration, electrolytic flotation, electrolytic precipitation and other methods; some uses firstly pre-oxidation treatment and then flocculation precipitation is carried out on the microalgae. The algae liquid separation technologies have the defects of high energy consumption, secondary pollution, difficult operation, influence of external factors (such as pH value of culture solution, temperature and the like) and difficult popularization and application in industrial production, and the centrifugation technology is mainly adopted in the industry at present to dehydrate algae. Therefore, the algae-liquid separation in the advanced wastewater treatment process is still a key difficulty restricting the popularization and application of the microalgae wastewater treatment technology.
[1]Molina G E,Belarbi E H,Acien Fernandez F G,et al.2003.Recovery of Microalgal Biomass and Metabolites:Process Options and Economics[J].Biotechnol.Adv.,(20):491-515。
[2]Uduman,N.and Y.Qi,et al.2010.Dewatering of microalgal cultures:a major bottleneck to algae-based fuels."Journal of renewable and sustainable energy 2(1):012701-16。
[3] Hu Yuansheng, Liu and Ha Xiao Di, etc. 2015 research status and prospect of flocculation separation/harvest in microalgae treated wastewater [ J ] science report on environmental sciences, 35(1): 12-29).
Disclosure of Invention
The invention aims to provide a method for applying calamus water extract to scenedesmus obliquus treatment wastewater to promote rapid separation of algae liquid, which realizes self-flocculation of scenedesmus obliquus in the wastewater treatment process by controlling the leaching time and content of the calamus water extract to accelerate the separation of the algae liquid. The method has the advantages of simple process, simple and convenient operation, low energy consumption, simple and convenient material acquisition, high efficiency, small land occupation area, no secondary pollution, strong practicability and convenient popularization and application.
The invention is realized by the following technical scheme:
a method for promoting rapid separation of algae liquid in microalgae wastewater treatment by using calamus water extract comprises the following steps:
(1) soaking rhizoma Acori Calami in water for 25-35 days, and filtering to obtain rhizoma Acori Calami water extractive solution;
(2) adding rhizoma Acori Calami water extractive solution into wastewater containing microalgae, culturing at 29 + -0.5 deg.C under illumination intensity of 3500lx and light-dark ratio of 14h:10h to promote formation of microalgae flocculating body and allow the microalgae flocculating body to settle.
Preferably, the calamus in the step (1) is cut to a section of 3-5cm, the water is deionized water, and the ratio of the calamus to the deionized water is 80g to 1L of the deionized water.
Preferably, the filtration in step (1) is filtration through a 0.45 μm microfiltration membrane.
Preferably, the content of the calamus water extract in the step (2) in the wastewater containing microalgae is 1g/L-4 g/L.
Preferably, the microalgae is scenedesmus obliquus.
Preferably, the wastewater is wastewater of a sewage treatment plant.
Preferably, the method further comprises the following steps:
(3) filtering to separate the microalgae flocculating constituent from the waste water completely.
The invention has the technical effects that: the microalgae flocculant has a strong microalgae self-flocculation sedimentation effect, can promote the growth of microalgae, and has the advantages of short self-flocculation time, quick sedimentation and obvious effect; the calamus resources are rich, the acquisition is simple and convenient, the technical requirement is lower, the operability is strong, and the popularization and the application of the technology are facilitated; the wastewater after being put into the algae treatment has no secondary pollution, is easy to degrade, is simple to process, and saves manpower, material resources and financial resources; the method has the advantages of low site requirement, simple operation, simple maintenance, low cost and strong popularization, and overcomes the problem of algae-liquid separation in the wastewater of algae treatment.
Drawings
FIG. 1 is a schematic diagram showing the effect of the content of the water extract of Acorus calamus on the inhibition rate of Scenedesmus obliquus in the example.
FIG. 2 is a photograph showing an example of the self-flocculation of Scenedesmus obliquus produced by the Acorus calamus water extract in the example.
Detailed Description
The following examples are provided to illustrate the advantageous effects of the present invention in order to help the reader to better understand the essence of the present invention, but should not be construed as limiting the scope of the present invention in any way.
Preparing water extract of calamus: harvesting fresh dry calamus on the lake bank of a certain park in Nanchang city, Jiangxi, cutting the selected calamus to a section of 3-5cm, soaking the section in deionized water, wherein the dosage of the calamus and the deionized water is 80g of calamus and 1L of distilled water, soaking for 25-35 days, and filtering through a 0.45 mu m microporous filter membrane to obtain the water extract of the calamus.
Collecting and measuring waste water: firstly, collecting the discharged wastewater of a sewage treatment plant, placing the wastewater in a room for later use, and measuring COD (chemical oxygen demand) of the wastewater by adopting a potassium dichromate methodCrTN is measured by potassium persulfate ultraviolet oxidation spectrophotometry, TP is measured by molybdenum antimony anti-spectrophotometry, ammonia nitrogen is measured by nano reagent photometry, and pH is measured by glass electrode method, which is specifically referred to in the methods of water and wastewater monitoring and analysis (fourth edition).
The calamus water extract promotes the formation of microalgae groups and accelerates sedimentation: adding the above rhizoma Acori Calami water extractive solution into the above wastewater at a ratio of 1g/L, controlling temperature at 29 + -0.5 deg.C, and culturing under controlled light with illumination intensity of 3500lx and light-dark ratio of 14h:10 h.
The result shows that the calamus water extract can promote the microalgae to form self-flocs and promote the separation of algae liquid, and the effect is good.
EXAMPLES of the invention
Firstly harvesting calamus on certain lake beach in south Jiangxi, cutting the selected calamus to a section of 3-5cm, putting the section into a 3000mL beaker, adding 2000mL deionized water, wherein the dosage of the calamus and the deionized water is 80g and 1L distilled water, and continuously soaking for 30 days under indoor normal temperature light shading conditions.
Taking out the water extract of the calamus, filtering the water extract through a 0.45-micron microporous filter membrane, and adding wastewater of a sewage treatment plant according to the concentration gradient of the soaking solution of the calamus, wherein the concentration gradient of the soaking solution of the calamus is 0g/L, 1g/L, 4g/L, 8g/L, 12g/L and 16g/L to obtain culture media with different calamus contents.
Culturing in an indoor temperature-controlled and light-controlled illumination incubator at 29 + -0.5 deg.C under illumination intensity of 3000lx and light-dark ratio of 14h:10h to obtain Scenedesmus obliquus form, sedimentation rate and inhibition rate, and calculating the sedimentation rate of rhizoma Acori Calami water extract on Scenedesmus obliquus, as shown in figure 1, figure 2 and table 1. From figure 1, it can be seen that scenedesmus obliquus growth in wastewater treatment in calamus water extract produces a phenomenon of "low promotion and high inhibition", and when the concentration is less than 1g/L, scenedesmus obliquus growth is promoted, which is improved by about 20%; the content of the calamus increases, the inhibition rate is increased along with the increase of the content of the calamus, and the inhibition rate is between 61 and 93 percent.
The calamus water extract produces self-flocculation to Scenedesmus obliquus in wastewater treatment (figure 2) to obtain Scenedesmus obliquus self-flocculent in500-12000 mu m, the sedimentation velocity is 1.8-3.5 multiplied by 10-3m/s, the calamus content is between 1g/L and 8g/L, the optimal Scenedesmus obliquus self-flocculation sedimentation effect is achieved, and the Scenedesmus obliquus growth is facilitated to absorb more nutrient salts. The content of the water extract of Acorus calamus is controlled at 8g/L and 12g/L, and is plotted by using self-flocculating constituent of Scenedesmus obliquus, as shown in FIG. 2. From FIG. 2 and Table 1, it can be seen that the calamus water extract promotes the formation of Scenedesmus obliquus self-flocs in wastewater treatment, and the sedimentation rate is about 0.25 cm/s. The content of calamus obtained by comprehensive analysis is 1g/L-4g/L, which is not only beneficial to the growth of scenedesmus obliquus, but also can promote the formation of scenedesmus obliquus self-flocs and promote the separation of algae liquid. Therefore, the calamus water extract can be used for performing self-flocculation on scenedesmus obliquus in wastewater treatment to promote algae-liquid separation, so that the technical bottleneck of algae-liquid separation in wastewater treatment by algae is overcome. The experimental parameters can meet the industrial application of scenedesmus obliquus in wastewater treatment.
TABLE 1 Scenedesmus obliquus sedimentation rates in different Acorus calamus biomass soaking solutions
The above-mentioned embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solution of the present invention by those skilled in the art should fall within the protection scope defined by the claims of the present invention without departing from the spirit of the present invention.
Claims (5)
1. A method for promoting rapid separation of algae liquid in microalgae wastewater treatment by using calamus water extract comprises the following steps:
(1) soaking rhizoma Acori Calami in water for 25-35 days, and filtering to obtain rhizoma Acori Calami water extractive solution;
(2) adding the calamus water extract into wastewater containing microalgae, culturing at 29 + -0.5 deg.C under the conditions of illumination intensity of 3500lx and light-dark ratio of 14h:10h to promote formation of microalgae flocculating body and allow the microalgae flocculating body to settle; wherein the content of the calamus water extract in the wastewater containing microalgae is 1 g/L; the microalgae is Scenedesmus obliquus.
2. The method of claim 1, wherein: cutting off the calamus in the step (1) to a section of 3-5cm, wherein the water is deionized water, and the ratio of the calamus to the deionized water is 80g to 1L of the deionized water.
3. The method of claim 1, wherein: the filtration in the step (1) is filtration through a 0.45 mu m microporous membrane.
4. The method of claim 1, wherein: the wastewater is wastewater of a sewage treatment plant.
5. The method according to any one of claims 1 to 4, wherein: further comprising the steps of:
(3) filtering to separate the microalgae flocculating constituent from the waste water completely.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011061596A3 (en) * | 2009-11-17 | 2012-01-19 | David Johannes Gaybba | Composition for use in water treatment |
| CN105129948A (en) * | 2015-09-06 | 2015-12-09 | 河南城建学院 | Method for extracting natural flocculation active ingredients from pomegranate bark and method for preparing flocculating agent |
| CN105254020A (en) * | 2015-11-17 | 2016-01-20 | 江西师范大学 | Method for quickly separating algae liquid in wastewater of microalgae treatment |
| CN106434354A (en) * | 2016-12-06 | 2017-02-22 | 暨南大学 | Oligomer flocculating microalgae utilization method and application method thereof |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011061596A3 (en) * | 2009-11-17 | 2012-01-19 | David Johannes Gaybba | Composition for use in water treatment |
| CN105129948A (en) * | 2015-09-06 | 2015-12-09 | 河南城建学院 | Method for extracting natural flocculation active ingredients from pomegranate bark and method for preparing flocculating agent |
| CN105254020A (en) * | 2015-11-17 | 2016-01-20 | 江西师范大学 | Method for quickly separating algae liquid in wastewater of microalgae treatment |
| CN106434354A (en) * | 2016-12-06 | 2017-02-22 | 暨南大学 | Oligomer flocculating microalgae utilization method and application method thereof |
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