CN108640262A - Magnetic Nano chitosan microball and preparation method thereof - Google Patents
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Abstract
The invention discloses a kind of magnetic Nano chitosan microballs and preparation method thereof, belong to magnetic Nano material preparing technical field, include the following steps：1. NaOH is added into ferric chloride solution, it is adjusted to alkalinity, filters out primary deposition, primary deposition is mixed with the dispersion phase, removal ethyl alcohol obtains the dispersed phase of primary deposition；The dispersed phase of the primary deposition is placed in autoclave to the dispersed phase that secondary precipitation is obtained by the reaction, gained secondary precipitation, that is, magnetic nanoparticle；2. the magnetic nanoparticle is added in chitosan solution, emulsifier and fixative is then added, it is isolated to precipitate three times, up to magnetic Nano chitosan microball after precipitation is dry three times by described in.The present invention is quasi- to provide a kind of magnetic nano-carrier, have the characteristics that it is good it is biological, with various active group, large specific surface area, porous, particle diameter distribution is narrow, it quickly can be moved and be detached under the action of externally-applied magnetic field, make microballoon have many advantages, such as easily recycle, it is renewable.
Description
Technical field
The present invention relates to magnetic Nano material preparing technical field, in particular to a kind of magnetic Nano chitosan microball and its
Preparation method.
Background technology
Cr (VI) is widely present in the industrial wastewaters such as leather manufacture, plating, printing, metallurgy, is had very to human body and environment
Big harmfulness is listed in first kind toxic contaminants element, therefore how effectively processing waste water containing chrome is current environmental protection
Important topic.Processing to chromate waste water mainly uses bioanalysis, ion-exchange, electrolysis, chemical precipitation method and suction at present
Attached method etc..
Common biological adsorption agent has bacterium, fungi, algae etc., study more microbial adsorbent have Zoogloea,
Arthrobacter, filamentous fungi, saccharomycete and algae, and less as the research of microbial adsorbent to sheath bacteria, mainly because
It is relatively difficult for isolating and purifying for sheath bacteria.Sheath bacteria (Sheathe Bacteria) is a kind of important filiform in activated sludge
Flora, can produce Fe-Mn oxidation enzyme, and form is in filar structure, non-limbed or have false ramification, and one is surrounded outside filamentous
Layer sheath, the key component of sheath is the organic matters such as protein, polysaccharide and lipid.Sheath bacteria, can profit to the of less demanding of nutritional condition
With a variety of simple organic compounds, such as alcohol, organic acid and carbohydrate, there are stronger tolerance and conversion capability to industrial wastewater.
In recent years some studies have shown that sheath bacteria not only in sewage organic matter and poisonous substance have very strong degradation, also have
The ability for adsorbing heavy metal all has good suction-operated to the various metals such as lead, copper, zinc, chromium, iron ion.In addition, sheath is thin
Bacterium can also secrete synthesis iron, manganese oxidase, these enzymes play critically important effect in sheath bacteria waste water control.Therefore, sheath
Bacterium is with a wide range of applications in heavy metal recovery, purification of water quality, environmental monitoring etc..
Using free viable bacteria body as biological adsorption agent, although with contact area is larger, mass-transfer performance preferably, reaction compared with
The advantages that rapid, but need to maintain the conditions such as certain temperature, nutrition and pH in adsorption process ensure microorganism itself
Growth, and viable bacteria body is used as adsorbent, physicochemical property there are certain defect, such as the later stage separation of solid and liquid difficulty,
The shortcomings of stability is poor, and adsorption strength is relatively low, it is difficult to realize industrial applications.Therefore a kind of suitable carrier loaded trip is selected
From cell, to improve the mechanical strength and biological stability of cell, it is easy to accomplish the separation of solid and liquid in later stage is current biological adsorption
One of the hot spot of agent research.
In recent years, with the fast development of nanosecond science and technology, more and more nano-oxides (such as titanium oxide, iron oxide)
Due to its superpower adsorption capacity to heavy metal, it is used as ecological restoration material, wherein magnetic Nano material is most widely used, most has
The adsorbent of effect.Skin effect specific to magnetic Nano material and magnetic response characteristic, make it as heavy metal ion adsorbed
When agent can high efficiente callback, easy to use, elution is simple, to avoid the secondary pollution to water body.Wherein iron oxide（Three oxidations two
Iron, ferroso-ferric oxide）It is most common nano adsorption material.But since magnetic Nano material has larger specific surface area,
Under the driving of skin effect, unmodified magnetic Nano material is easily reunited, therefore often need to be to magnetic Nano material surface
It is modified, such as silica, activated carbon, high molecular polymer substance are common decorative material.Magnetism after modification is received
Rice material further grafts some groups, such as carboxyl, hydroxyl, sulfate radical, phosphate radical, amide and amino function on the surface thereof
Group not only increases the stability of magnetic nano-particle in this way, also improves absorption of the magnetic Nano material to metal ion
Selectivity and adsorption capacity.
Chitosan（Chitosan）Be by the product also known as chitin after chitin (Chitin) deacetylation, chitin,
It is many higher mammals, especially the important component of arthropod (such as shrimp, crab) shell, while existing in rudimentary plant
As fungi cell wall in, be widely distributed.This natural macromolecular material has good biocompatibility and blood phase
Capacitive, and be easily modified, molecular surface contains a large amount of amino（-NH2）And hydroxyl（-OH）Isoreactivity group, can with again
Metal ion forms stable chelate.
Suitable carrier is needed when sheath bacteria is applied to sewage disposal, and prepares the carrier and works as what is applied at present
Business is anxious.
Invention content
In view of the above technical problems, magnetic Nano chitosan microball of the present invention and preparation method thereof is quasi- provides one kind
It is suitble to the magnetic nano-carrier of load sheath bacteria S9, makes it have there are many good biocompatibility and biological degradabilities, band
Active group while having the characteristics that the large specific surface area of magnetic responsiveness and nano-particle, porous, particle diameter distribution is narrow, outside plus
Quickly can be moved and be detached under the action of magnetic field, make microballoon have many advantages, such as easily recycle, it is renewable.
To achieve the above object, the preparation method of the magnetic Nano chitosan microball designed by the present invention, including walk as follows
Suddenly：
1. the preparation of magnetic nanoparticle：Alkaline matter is added into ferric chloride solution, its pH value is adjusted to 11 ~ 13, is waited for molten
Primary deposition is filtered out after liquid layering, primary deposition is placed in absolute ethyl alcohol and is disperseed；
Dispersion liquid is prepared, the primary deposition that will be dispersed in absolute ethyl alcohol is mixed with the dispersion phase, is dried after mixing to go
Except absolute ethyl alcohol, the dispersed phase of primary deposition is obtained；
The dispersed phase of the primary deposition is placed in autoclave, reacts 10 ~ 14 hours, obtains under conditions of 210 ~ 250 DEG C
The dispersed phase of secondary precipitation；
The dispersed phase of the secondary precipitation is centrifuged to collect secondary precipitation, is dried to get magnetic nanoparticle after washing；
2. the preparation of magnetic Nano chitosan microball：The magnetic nanoparticle is added in chitosan solution, breast is then added
Agent, stirring is abundant, and fixative is then added, and is again stirring for fully, being centrifugally separating to obtain and precipitating three times, precipitate described three times
Up to magnetic Nano chitosan microball after drying.
As a preferred embodiment of the above technical solution, 1. the alkaline matter is sodium hydroxide solution to step.
As a preferred embodiment of the above technical solution, 1. step passes through after the primary deposition and the secondary precipitation are separated
Cross the alternating washing of deionized water and absolute ethyl alcohol；Step 2. described in three times precipitation separate after using deionized water carry out
Washing.
As a preferred embodiment of the above technical solution, step 1. the dispersion liquid be octadecylene and oleic acid mixed liquor, described ten
The volume ratio of eight alkene and the oleic acid is 1.4 ~ 1.6:1.
As a preferred embodiment of the above technical solution, step 1. in will be dispersed in primary deposition in absolute ethyl alcohol and the dispersion
Liquid phase mixes, and drying in 5 ~ 6 hours is dried under the conditions of 100 DEG C to remove absolute ethyl alcohol after mixing.
As a preferred embodiment of the above technical solution, step 1. described in secondary precipitation be collected by centrifugation condition be 5000r/min
Centrifugation 10 minutes.
As a preferred embodiment of the above technical solution, step 2. described in chitosan in chitosan solution and the magnetic Nano
The mass ratio of particle is 150 ~ 170:1.
As a preferred embodiment of the above technical solution, step 2. described in emulsifier be Span-80, addition be every milligram of magnetism
1ml Span-80 are added in nano particle.
As a preferred embodiment of the above technical solution, step 2. described in fixative be that the glutaraldehyde that volume fraction is 4% is water-soluble
Liquid, addition are the glutaraldehyde water solution that every milligram of magnetic nanoparticle is added that 5ml volume fractions are 4%.
Magnetic Nano chitosan microball, prepared by the preparation method by above-mentioned magnetic Nano chitosan microball.
The embodiment of the present invention compared with prior art, has the following advantages and advantageous effect：
The embodiment of the present invention is quasi- to provide a kind of magnetic nano-carrier of suitable load sheath bacteria S9, makes it have good biology
Compatibility and biological degradability with various active group while having the large specific surface area, more of magnetic responsiveness and nano-particle
The features such as hole, narrow particle diameter distribution, quickly can be moved and be detached under the action of externally-applied magnetic field, make microballoon have easily recycling,
The advantages that renewable；
Sheath bacteria S9 is the main Filamentous flora in activated sludge, cell surface contain a large amount of amide groups, hydroxyl, amino,
Carboxyl, sulfydryl isoreactivity group, have tolerance to various metals ion, have good absorption property to hexavalent chromium,
Since the stability of free cell is poor, the later stage be separated by solid-liquid separation it is difficult, therefore using being combined with receiving for chitosan and Fe3O4 advantages
Rice chitosan magnetic loads sheath bacteria S9, while increasing the mechanical strength and chemical stability of sheath bacteria cell,
Also the advantages of sheath bacteria S9 is with chitosan and Fe3O4 is assigned, absorption of the sheath bacteria S9 to Cr (VI) ion is greatly improved
Performance, for the industrial improvement containing Cr (VI) ion waste water provide a kind of novel, high-adsorption-capacity, stabilization biological adsorption
Agent.
Specific implementation mode
With reference to specific embodiment, the present invention is described in further detail：
Embodiment 1：The preparation method of magnetic Nano chitosan microball, includes the following steps：
1. the preparation of magnetic nanoparticle：The sodium hydroxide of 3mol/L is added dropwise into the ferric chloride solution of 80ml, 0.0375mol/L
Its pH value is adjusted to 12 by aqueous solution, and primary deposition is filtered out after solution layering, is alternately washed with absolute ethyl alcohol and deionized water
3 times, primary deposition is placed in absolute ethyl alcohol and is disperseed using ultrasound；
It is configured to dispersion liquid after 20ml octadecylenes and 13.5ml oleic acid are mixed ultrasonic disperse, will be dispersed in absolute ethyl alcohol
Primary deposition is mixed with the dispersion phase, again ultrasound 30 minutes, is placed at 100 DEG C dry 5.5 hours removal absolute ethyl alcohols,
Obtain the dispersed phase of primary deposition；
The dispersed phase of the primary deposition is placed in autoclave, is reacted 12 hours under conditions of 230 DEG C, obtains secondary precipitation
Dispersed phase；
After its cooling, the dispersed phase of the secondary precipitation is carried out centrifuging 10 minutes to collect under conditions of 5000r/min
Secondary precipitation, absolute ethyl alcohol and deionized water spontaneously dry after alternately washing to get magnetic nanoparticle；
2. the preparation of magnetic Nano chitosan microball：It is added in the chitosan solution of a concentration of 4g/L of 4ml magnetic described in 0.1mg
Nano particle, is then added 0.1mlSpan-80, then it is water-soluble that the glutaraldehyde that volume fraction is 4% is added in magnetic agitation 1 hour
Liquid stirs 2 hours under conditions of 350r/min, is centrifugally separating to obtain and precipitates three times, deionized water is used in combination to be washed, will wash
Up to magnetic Nano chitosan microball after the drying of precipitation three times after washing, magnetic Nano chitosan microball is put to refrigerator and is protected
It deposits.
Embodiment 2：The preparation method of magnetic Nano chitosan microball, includes the following steps：
1. the preparation of magnetic nanoparticle：The sodium hydroxide of 3mol/L is added dropwise into the ferric chloride solution of 80ml, 0.0375mol/L
Its pH value is adjusted to 11 by aqueous solution, and primary deposition is filtered out after solution layering, is alternately washed with absolute ethyl alcohol and deionized water
3 times, primary deposition is placed in absolute ethyl alcohol and is disperseed using ultrasound；
It is configured to dispersion liquid after 20ml octadecylenes and 14.3ml oleic acid are mixed ultrasonic disperse, will be dispersed in absolute ethyl alcohol
Primary deposition is mixed with the dispersion phase, again ultrasound 30 minutes, is placed at 100 DEG C dry 5 hours removal absolute ethyl alcohols, is obtained
To the dispersed phase of primary deposition；
The dispersed phase of the primary deposition is placed in autoclave, is reacted 14 hours under conditions of 210 DEG C, obtains secondary precipitation
Dispersed phase；
After its cooling, the dispersed phase of the secondary precipitation is carried out centrifuging 10 minutes to collect under conditions of 5000r/min
Secondary precipitation, absolute ethyl alcohol and deionized water spontaneously dry after alternately washing to get magnetic nanoparticle；
2. the preparation of magnetic Nano chitosan microball：It is added described in 0.1mg in the chitosan solution of a concentration of 3.75g/L of 4ml
Magnetic nanoparticle, is then added 0.1mlSpan-80, then the glutaraldehyde that volume fraction is 4% is added in magnetic agitation 1 hour
Aqueous solution stirs 2 hours under conditions of 350r/min, is centrifugally separating to obtain and precipitates three times, deionized water is used in combination to be washed,
Up to magnetic Nano chitosan microball, magnetic Nano chitosan microball will be put to ice after precipitation is dry three times described in after washing
Case preserves.
Embodiment 3：The preparation method of magnetic Nano chitosan microball, includes the following steps：
1. the preparation of magnetic nanoparticle：The sodium hydroxide of 3mol/L is added dropwise into the ferric chloride solution of 80ml, 0.0375mol/L
Its pH value is adjusted to 13 by aqueous solution, and primary deposition is filtered out after solution layering, is alternately washed with absolute ethyl alcohol and deionized water
3 times, primary deposition is placed in absolute ethyl alcohol and is disperseed using ultrasound；
It is configured to dispersion liquid after 20ml octadecylenes and 12.5ml oleic acid are mixed ultrasonic disperse, will be dispersed in absolute ethyl alcohol
Primary deposition is mixed with the dispersion phase, again ultrasound 30 minutes, is placed at 100 DEG C dry 6 hours removal absolute ethyl alcohols, is obtained
To the dispersed phase of primary deposition；
The dispersed phase of the primary deposition is placed in autoclave, is reacted 10 hours under conditions of 250 DEG C, obtains secondary precipitation
Dispersed phase；
After its cooling, the dispersed phase of the secondary precipitation is carried out centrifuging 10 minutes to collect under conditions of 5000r/min
Secondary precipitation, absolute ethyl alcohol and deionized water spontaneously dry after alternately washing to get magnetic nanoparticle；
2. the preparation of magnetic Nano chitosan microball：It is added described in 0.1mg in the chitosan solution of a concentration of 4.25g/L of 4ml
Magnetic nanoparticle, is then added 0.1mlSpan-80, then the glutaraldehyde that volume fraction is 4% is added in magnetic agitation 1 hour
Aqueous solution stirs 2 hours under conditions of 350r/min, is centrifugally separating to obtain and precipitates three times, deionized water is used in combination to be washed,
Up to magnetic Nano chitosan microball, magnetic Nano chitosan microball will be put to ice after precipitation is dry three times described in after washing
Case preserves.
Embodiment 4：The preparation method of magnetic Nano chitosan microball, includes the following steps：
1. the preparation of magnetic nanoparticle：The sodium hydroxide of 3mol/L is added dropwise into the ferric chloride solution of 80ml, 0.0375mol/L
Its pH value is adjusted to 12 by aqueous solution, and primary deposition is filtered out after solution layering, is alternately washed with absolute ethyl alcohol and deionized water
3 times, primary deposition is placed in absolute ethyl alcohol and is disperseed using ultrasound；
It is configured to dispersion liquid after 20ml octadecylenes and 13.2ml oleic acid are mixed ultrasonic disperse, will be dispersed in absolute ethyl alcohol
Primary deposition is mixed with the dispersion phase, again ultrasound 30 minutes, is placed at 100 DEG C dry 6 hours removal absolute ethyl alcohols, is obtained
To the dispersed phase of primary deposition；
The dispersed phase of the primary deposition is placed in autoclave, is reacted 11 hours under conditions of 240 DEG C, obtains secondary precipitation
Dispersed phase；
After its cooling, the dispersed phase of the secondary precipitation is carried out centrifuging 10 minutes to collect under conditions of 5000r/min
Secondary precipitation, absolute ethyl alcohol and deionized water spontaneously dry after alternately washing to get magnetic nanoparticle；
2. the preparation of magnetic Nano chitosan microball：It is added in the chitosan solution of a concentration of 4g/L of 4ml magnetic described in 0.1mg
Nano particle, is then added 0.1mlSpan-80, then it is water-soluble that the glutaraldehyde that volume fraction is 4% is added in magnetic agitation 1 hour
Liquid stirs 2 hours under conditions of 350r/min, is centrifugally separating to obtain and precipitates three times, deionized water is used in combination to be washed, will wash
Up to magnetic Nano chitosan microball after the drying of precipitation three times after washing, magnetic Nano chitosan microball is put to refrigerator and is protected
It deposits.
Embodiment 5：The preparation method of magnetic Nano chitosan microball, includes the following steps：
1. the preparation of magnetic nanoparticle：The sodium hydroxide of 3mol/L is added dropwise into the ferric chloride solution of 80ml, 0.0375mol/L
Its pH value is adjusted to 12 by aqueous solution, and primary deposition is filtered out after solution layering, is alternately washed with absolute ethyl alcohol and deionized water
3 times, primary deposition is placed in absolute ethyl alcohol and is disperseed using ultrasound；
It is configured to dispersion liquid after 20ml octadecylenes and 13.5ml oleic acid are mixed ultrasonic disperse, will be dispersed in absolute ethyl alcohol
Primary deposition is mixed with the dispersion phase, again ultrasound 30 minutes, is placed at 100 DEG C dry 6 hours removal absolute ethyl alcohols, is obtained
To the dispersed phase of primary deposition；
The dispersed phase of the primary deposition is placed in autoclave, is reacted 12 hours under conditions of 210 DEG C, obtains secondary precipitation
Dispersed phase；
After its cooling, the dispersed phase of the secondary precipitation is carried out centrifuging 10 minutes to collect under conditions of 5000r/min
Secondary precipitation, absolute ethyl alcohol and deionized water spontaneously dry after alternately washing to get magnetic nanoparticle；
2. the preparation of magnetic Nano chitosan microball：Magnetic described in 0.1mg is added in the chitosan solution of a concentration of 4.1g/L of 4ml
Property nano particle, be then added 0.1mlSpan-80, then the glutaraldehyde water that volume fraction is 4% is added in magnetic agitation 1 hour
Solution stirs 2 hours under conditions of 350r/min, is centrifugally separating to obtain and precipitates three times, deionized water is used in combination to be washed, will
Up to magnetic Nano chitosan microball after the drying of precipitation three times after washing, magnetic Nano chitosan microball is put to refrigerator
It preserves.
Embodiment 6 ~ 10：Respectively prepared by the preparation method of the magnetic Nano chitosan microball described in corresponding embodiment 1 ~ 5
Magnetic Nano chitosan microball.
Claims (10)
1. the preparation method of magnetic Nano chitosan microball, which is characterized in that include the following steps：
1. the preparation of magnetic nanoparticle：Alkaline matter is added into ferric chloride solution, its pH value is adjusted to 11 ~ 13, is waited for molten
Primary deposition is filtered out after liquid layering, primary deposition is placed in absolute ethyl alcohol and is disperseed；
Dispersion liquid is prepared, the primary deposition that will be dispersed in absolute ethyl alcohol is mixed with the dispersion phase, is dried after mixing to go
Except absolute ethyl alcohol, the dispersed phase of primary deposition is obtained；
The dispersed phase of the primary deposition is placed in autoclave, reacts 10 ~ 14 hours, obtains under conditions of 210 ~ 250 DEG C
The dispersed phase of secondary precipitation；
The dispersed phase of the secondary precipitation is centrifuged to collect secondary precipitation, is dried to get magnetic nanoparticle after washing；
2. the preparation of magnetic Nano chitosan microball：The magnetic nanoparticle is added in chitosan solution, breast is then added
Agent, stirring is abundant, and fixative is then added, and is again stirring for fully, being centrifugally separating to obtain and precipitating three times, precipitate described three times
Up to magnetic Nano chitosan microball after drying.
2. the preparation method of magnetic Nano chitosan microball according to claim 1, it is characterised in that：The step 1. alkali
Property substance be sodium hydroxide solution.
3. the preparation method of magnetic Nano chitosan microball according to claim 1, it is characterised in that：Step is 1. described first
The alternating washing of deionized water and absolute ethyl alcohol is passed through in secondary precipitation and the secondary precipitation after separating；Step 2. described in
Precipitation is washed after separating using deionized water three times.
4. the preparation method of magnetic Nano chitosan microball according to claim 1, it is characterised in that：1. described point of step
Dispersion liquid is the mixed liquor of octadecylene and oleic acid, and the volume ratio of the octadecylene and the oleic acid is 1.4 ~ 1.6:1.
5. the preparation method of magnetic Nano chitosan microball according to claim 1, it is characterised in that：Step 1. in will point
The primary deposition being dispersed in absolute ethyl alcohol is mixed with the dispersion phase, the dry drying in 5 ~ 6 hours under the conditions of 100 DEG C after mixing
To remove absolute ethyl alcohol.
6. the preparation method of magnetic Nano chitosan microball according to claim 1, it is characterised in that：Step 1. described in
The condition that is collected by centrifugation of secondary precipitation is that 5000r/min is centrifuged 10 minutes.
7. the preparation method of magnetic Nano chitosan microball according to claim 1, it is characterised in that：Step 2. described in
The mass ratio of chitosan and the magnetic nanoparticle in chitosan solution is 150 ~ 170:1.
8. the preparation method of magnetic Nano chitosan microball according to claim 1, it is characterised in that：Step 2. described in
Emulsifier is Span-80, and addition is that 1ml Span-80 are added in every milligram of magnetic nanoparticle.
9. the preparation method of magnetic Nano chitosan microball according to claim 1, it is characterised in that：Step 2. described in
Fixative is the glutaraldehyde water solution that volume fraction is 4%, and addition is that 5ml volume fractions are added in every milligram of magnetic nanoparticle
For 4% glutaraldehyde water solution.
10. magnetic Nano chitosan microball, it is characterised in that：It is poly- by the magnetic Nano shell described in any bar in claim 1 ~ 9
Prepared by the preparation method of sugared microballoon.
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| CN109603765B (en) * | 2018-12-14 | 2021-07-16 | 陕西师范大学 | Magnetic bead of chitosan oligosaccharide, preparation method and application thereof |
| CN109699687A (en) * | 2019-02-27 | 2019-05-03 | 陕西省微生物研究所 | A kind of preparation method of environment-friendly type pyrethrin control-release microsphere |
| CN110627509A (en) * | 2019-10-11 | 2019-12-31 | 航天特种材料及工艺技术研究所 | Preparation method of aluminum nitride powder |
| CN110627509B (en) * | 2019-10-11 | 2022-03-15 | 航天特种材料及工艺技术研究所 | Preparation method of aluminum nitride powder |
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