CN108633918A - Using nimbin and aalii total saposins as Pesticidal combination of active constituent and usage thereof - Google Patents
Using nimbin and aalii total saposins as Pesticidal combination of active constituent and usage thereof Download PDFInfo
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- CN108633918A CN108633918A CN201810375146.2A CN201810375146A CN108633918A CN 108633918 A CN108633918 A CN 108633918A CN 201810375146 A CN201810375146 A CN 201810375146A CN 108633918 A CN108633918 A CN 108633918A
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- aalii
- nimbin
- total saposins
- diamondback moth
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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Abstract
Using nimbin and aalii total saposins as Pesticidal combination of active constituent and usage thereof, belong to the pest control field during crop-planting.Composition contains active component nimbin and aalii total saposins, and the weight ratio of nimbin and aalii total saposins is 1:0.1~10.Most preferably, the weight ratio of nimbin and aalii total saposins is 1:6.38.Composition is used for pest control in agricultural production, it is preferable that for preventing lepidoptera pest, is especially used to prevent diamondback moth (Plutella xylostella L.).Aalii total saposins close mutual relative potencies with print chinaberry extremely strong humidification, is greatly improved to agricultural insect management effect, effect is far superior to used alone aalii total saposins or nimbin is used alone, and can significantly reduce pesticide cost, widens use scope.
Description
Technical field
The invention belongs to the pest control fields during crop-planting, and in particular to one kind is with nimbin and aalii total saposins
For the Pesticidal combination and usage thereof of active constituent.
Background technology
Nimbin (Azadirachtin) is to separate highly active compound in a kind of chinaberry from print, it belongs to Fourth Ring
Triterpenes.It prints in fruit, seed, seed nucleus, branch, leaf, bark and the sap of chinaberry and all contains active material, so far, print in chinaberry
Have found that 100 multiple compounds, at least 70 kinds of compounds have bioactivity, predominantly margosa tree element, China tree three
Alcohol and nimbin etc., these extracts to insect have food refusal, interference oviposition, interference insect make a variation, make it that can not change in quality for adult,
Repellent larva and inhibit growth effect and reach desinsection purpose.
Nimbin has become a kind of biological pesticide common at this stage, in the biological prevention and control neck of the pests such as prevention diamondback moth
It is used widely in domain.Applicant has the research on certain basis, patent in the extraction of aalii total saposins and application field
CN201310075529.5 has shown that prevention and control principle of the aalii total saposins to diamondback moth.
Diamondback moth (Plutella xylostella L.) belongs to lepidoptera diamond-back moth section, alias square moth, two cephalonts.Diamondback moth
For worldwide pest, vegetables production district in all parts of the country, which has, to cause harm, and is attached most importance to southern various regions.Diamondback moth mainly endangers leaf with larva
Based on piece.Blade can be eaten into hole and incised by 3~4 instar larvaes, eaten blade when serious and reticulated, seriously affect yield of vegetables
And quality.
If nimbin is shared with another active constituent there can be positive synergistic effect, there is synergy, can greatly improve
To the control effect of the pests such as diamondback moth, effect is far superior to used alone, then has important energy economy & environment meaning.
Aalii Dodonaea voscosa (Linn.) Jacq also known as akeake, bright foxy old hand, lopseed, iron broom, pine torch
Bavin, parched rice bavin, hair breast, white stone chinaberry, small stream willow, belong to Sapindaceae shrub or dungarunga, high 1~5m, bark sepia, single leaf are mutual
Raw, film quality, leaf is slightly larger, long 7~10cm, wide 1.5~2cm.Shape size difference is big, and oblong, narrow lanceolar fall lanceolar extremely
Wire lanceolar, long 5~12cm, wide 0.5~4cm.Panicle or raceme, basidixed or in sprig top axillary are shorter
Or nearly stockless.Main application and pharmacological effects:Seed oil can make soap, and civil to be used for lighting, leaf is clearing heat and detoxicating, and anti-inflammatory stops
Cough, can also control scald, and complete stool is controlling rheumatism.Aalii seed ethanol extract has good food refusal to diamondback moth, cabbage caterpillar etc.
Activity, saponin(e are the main actives in aalii seed, and it is triterpenoid saponin to have the active constituent of Antifeedant Effects to diamondback moth.
So far there are no, and document report nimbin is shared with aalii total saposins with by force building performance.
Invention content
The purpose of the present invention is to provide using nimbin and aalii total saposins as the Pesticidal combination of active constituent and its use
Method.
Applicant has been surprisingly found that aalii total saposins share tool with print chinaberry in the later stage R&D work to nimbin
There is extremely strong synergistic effect, the control effect to insect pests such as diamondback moths can be greatly improved, aalii is far superior to used alone in effect
Total saposins are used alone nimbin, are found by a series of experiments and determine in the best mixtures' ratio of prevention diamondback moth.
Pesticidal combination of the present invention is characterized in that containing active component nimbin and aalii total saposins, nimbin and aalii
The weight ratio of total saposins is 1:0.1~10.Most preferably, the weight ratio of nimbin and aalii total saposins is 1:6.38.
The various regular dosage forms of insecticide can be made in Pesticidal combination of the present invention.Pesticidal combination of the present invention can contain
Accordingly the conventional non-active ingredient needed for dosage form, the pharmaceutically various auxiliary materials of acceptable.
The usage of Pesticidal combination of the present invention is for pest control in agricultural production, and occupation mode includes but not limited to spray
Spill, brush, inducing feeding etc..Preferably, Pesticidal combination of the present invention is especially used to prevent small for preventing lepidoptera pest
Diamond-back moth (Plutella xylostella L.).
Advantages of the present invention:Aalii total saposins close mutual relative potencies with print chinaberry extremely strong humidification, can be greatly improved
To agricultural insect management effect, effect is far superior to used alone aalii total saposins or nimbin is used alone, can be significantly
Pesticide cost is reduced, use scope is widened.
Specific implementation mode
See following instance:
1. materials and methods
1.1 material
Reagent agent:50% nimbin raw medicine:Yunnan Zhongke Bio-Industry Co., Ltd provides;Aalii total saposins:Yunnan
Agriculture university's botanical pesticide laboratory is prepared according to patent ZL201310075529.5 methods.
Selected insect source:Diamondback moth Plutella xylostella.L, from being connected on the wild cabbage of diamondback moth for laboratory autotrophy
The pupa for collecting diamondback moth maturation, is put into triangular flask, is inserted into the self-control blowing paper production for being coated with wild cabbage juice into bottle after sprouting wings
Ovum plate is used in combination 10% hydromel to feed adult, replaces oviposition plate and hydromel daily, and oviposition plate is put into 26 ± 1 DEG C
Moisturizing is hatched in incubator.The oviposition plate hatched is placed in fresh dish leaf, egg hatching independently takes in dish leaf at larva
Food, according to circumstances replaces dish leaf, waits for that larvae development was for experiment to 3 ages.
1.2 test method
1.2.1 the AFC of single dose50Value measures
Using leaf butterfly method, on the basis of prerun, active compound is dissolved with suitable solvent, and be configured to the medicine of 5~6 concentration
Liquid.Fresh more smooth uniform cabbage leaves are broken into leaf dish with round card punch (Φ 1.8cm), is put into extract liquid and soaks
2~3s of stain (control coordinative solvent).It dries at room temperature, is put into the culture dish of diameter 9cm that (interior pad filter paper, adds a small amount of distillation
Water moisturizing), it is often equidistant in ware to put 4 leaf dish.It is equidistant in every ware to be put into 4 processing in non-selective Antifeedant Effects experiment
Leaf dish, cross type arrangement, 4 another wares of control leaf dish are placed.3 instar larvae of diamondback moth for accessing starvation 4h, per 1 conduct of ware
Primary to repeat, each processing sets 20 repetitions, is control with proper amount of acetone plus clear water processing.For examination larva 25 ± 1 DEG C of temperature,
Relative humidity 75%~85% is raised in the illumination box of daily illumination 16h, measures remaining leaf with squared paper afterwards for 24 hours
Area calculates anti-food rate according to the following formula.Then anti-food rate is converted to probit value, concentration is converted into logarithm and is linearly returned
Return, according to concentration (AFC in regression equation calculation food refusal50)。
1.2.2 the screening of mixture optimum proportioning
Using mixing method in proportion.The AFC of 2 kinds of single doses is determined first50Value, with respective AFC50Value is 100%, then 5
Decile calculates separately the content in each ratio, then 2 kinds of single doses is pressed (a+0), [(4/5) a+ (1/5) b], [(3/5) a+ (2/
5) b], [(2/5) a+ (3/5) b], [(1/5) a+ (4/5) b], (ten b of O) mixing measure food refusal of each mix ratio group to diamondback moth
Activity.
1.2.3 the measurement of synergistic effect
The co-toxicity coefficient of each mixture is calculated with reference to Sun-JohnSon methods, if the co-toxicity coefficient of mixture indicates this close to 100
Mixture effect is similar to synergy;If co-toxicity coefficient is noticeably greater than 100, synergistic effect is indicated;If co-toxicity coefficient is less than
100, then it represents that have antagonism:
The AFC of confection actual toxicity index (ATI)=standard agent50The AFC of/confection50×100
Toxicity index × A of confection theoretical toxicity index (TTI)=A medicaments accounts for the percentage+B medicines during mixing assembles
Toxicity index × B of agent accounts for the percentage during mixing assembles
Co-toxicity coefficient (CTC)=confection actual toxicity index (ATI)/confection theoretical toxicity index (TTI) ×
100
1.3 data analysing method
Analyzing processing is carried out to test data using SPSS16.0,
2 results and analysis
2.1 print chinaberrys are with aalii total saposins to the antifeedant activity of diamondback moth
Measure AFC of the print chinaberry active compound to diamondback moth50For 0.278g/L, aalii total saposins are to the AFC50 of diamondback moth
0.4437g/L is shown in Table 1
Table 1 prints AFC of the chinaberry with diamondback moth to diamondback moth50Value
2.1 mixture co-toxicity coefficients measure and optimum proportioning screening
Toxicity Determination is carried out to each proportioning respectively, finds out respective virulence regression equation and AFC50Value, and combine two
The AFC of single dose50Value calculates co-toxicity coefficient.As can be seen from Table 2, the co-toxicity coefficient of each mixture processing is all higher than 100, shows
For synergistic effect.Wherein mixture 2, i.e. aalii total saposins and the ratio of print chinaberry are 6.38:When 1, co-toxicity coefficient is up to 688.74,
It is far longer than 100, synergistic effect is the most notable.
2 aalii saponin(e of table with it is nimbin mixed to the coefficient of synergism of 3 instar larvae antifeedant activity of diamondback moth compared with
Note:A:Aalii total saposins, B:Nimbin
Above the experimental results showed that print chinaberry is a kind of biogenic pesticide having the environmental protection compared with high bioactivity to a variety of biologies,
It is compatible with the environment, is nuisanceless, insecticidal mechanism is unique, active high, superior performance broad spectrum pesticide, can avoid chemical pesticide band
The disadvantage come, meets the insecticide of " green " crops of current World Health organizational requirements.But its cost is higher to cause its use
It is subject to certain restrictions.This experimental study shows:Print chinaberry shares with good synergistic effect, especially with aalii total saposins
The two ratio is 1:When 2.394, co-toxicity coefficient is up to 688.74, and synergy is the most apparent.Print chinaberry active compound can be substantially reduced in this way
Usage amount.
3. aalii total saposins are to diamondback moth Antifeedant Effect Against result
3.1 non-selective Antifeeding Effects
3 aalii total saposins of table are to 3 instar larvae non selective antifeedant activity of diamondback moth
Note:There is same letter to indicate under 95% level without significant difference (P=0.05) in table after data
The test result of table 3 shows that same aalii total saposins concentration for the treatment of, for 24 hours, the anti-food rate of 48h, 72h are gradual for processing
Decline, various concentration handling result and concentration gradient are proportionate trend.It can be seen that, under 2.00g/L concentration, handled in table
Anti-food rate for 24 hours is up to 90.03%, and after handling 72h, anti-food rate is reduced to 88.34%.
3.2 regression equation
4 aalii total saposins of table are to 3 instar larvae non selective antifeedant activity virulence of diamondback moth
Note:There is same letter to indicate under 95% level without significant difference (P=0.05) in table after data
It can see from table 4, AFC of the aalii total saposins to 3 instar larvae of diamondback moth50Value for 24 hours, distinguish by when 48h, 72h
For 0.4437g/L, 0.4748g/L, 0.6605g/L, show an ascendant trend, i.e. antifeedant activity gradually weakens.In addition, we
It can further be seen that being gradually risen from the P values that 0.9579, T is examined are reduced to from 0.9918 to 72h related coefficients for 24 hours.
4. printing antifeedant activity of the chinaberry active compound to diamondback moth larvae
Table 5 prints anti-food rate of the chinaberry to diamondback moth larvae
Note:There is same letter to indicate under 95% level without significant difference (P=0.05) in table after data
It can be seen that, print chinaberry is very high to the anti-food rate of diamondback moth larvae, and under 1g/L concentration, anti-food rate is for 24 hours in table 5
88.21%, 48h 90.24%, 72h 93.77%.It is in rising trend, and between various concentration, in identical processing time,
Anti-food rate and concentration level are proportionate trend, and otherness is apparent between each processing.During the test, we can also see
It arrives, after processing for 24 hours, the pickles larvae no longer feeding substantially of high concentration processing, some diamondback moth larvae polypides reduce, and head is extremely
Anterior part of chest color burn, blackening are dead.This with it has been reported that print chinaberry food refusal mechanism be obstruction insect lancet, lead to insect
Can not feeding death be consistent.
Table 6 prints selective antifeedant activity Virulent Analysis of the chinaberry to diamondback moth larvae
Table 6 is the antifeedant activity Virulent Analysis for printing chinaberry to diamondback moth, is handled for 24 hours, the AFC of 48h, 72h50Respectively
0.293g/L, 0.2027g/L, 0.0926g/L.Wherein, the AFC of 72h is handled50Minimum, Antifeeding Effects are best.
5. aalii total saposins and print chinaberry active compound mixed synergies measurement result
A:Aalii total saposins, B:Nimbin.Refer to the weight ratio of aalii saponin(e and nimbin, 1 (A of mixture:B) it is
6.38:1,2 (A of mixture:B it is) 2.394:1,3 (A of mixture:B it is) 1.06:1,4 (A of mixture:B it is) 1:2.506.5.1.1 mixture 1
To the non-selective Antifeeding Effects of diamondback moth larvae
Non-selective Antifeeding Effects of 7 mixture 1 of table to diamondback moth larvae
Note:There is same letter to indicate under 95% level without significant difference (P=0.05) in table after data
In table 7 it can be seen that, under maximum concentration, for 24 hours when, anti-food rate 74.39%;48h is that 79.34%, 72h is
81.04%.In low concentration 0.051g/L, 0.026g/L, anti-food rate is minimum when for 24 hours, and respectively 25.07%, 5.90%, 48h
Highest, and to 72h when, anti-food rate is reduced to 28.72%, 0.93%.Same processing time, with the reduction of concentration for the treatment of,
Anti-food rate also reduces.
5.1.2 selective antifeedant activity Virulent Analysis of the mixture 1 to diamondback moth larvae
Non- antifeedant activity Virulent Analysis of 8 mixture 1 of table to 3 instar larvae of diamondback moth
By table 8, it can be seen that, mixture 1 is preferable to the non-selective Antifeeding Effects of diamondback moth larvae, handles for 24 hours, 48h, 72h
AFC50Respectively 0.155g/L, 0.0699g/L, 0.1236g/L, wherein handle the AFC of 48h50It is worth minimum, Antifeeding Effects are most
It is good.
Non-selective Antifeeding Effects of 5.2 mixtures 2 to diamondback moth larvae
5.2.1 Antifeeding Effects of the mixture 2 to diamondback moth larvae
Non-selective Antifeeding Effects of 9 mixture 2 of table to 3 instar larvae of diamondback moth
Note:There is same letter to indicate under 95% level without significant difference (P=0.05) in table after data
Table 9 shows under maximum concentration that for 24 hours, the anti-food rate of 48h, 72h are difference 83.29%, 90.26% for processing,
89.65%, 48h anti-food rate highest.Such a trend is presented in 5 concentration for the treatment of, i.e. food refusal takes the lead in subtracting after increasing, and is in 48h
Highest, 72h are reduced again.But it is higher than anti-food rate for 24 hours.Same processing time, anti-food rate are reduced as concentration reduces.Difference is bright
It is aobvious.
5.2.2 selective antifeedant activity Virulent Analysis of the mixture 2 to diamondback moth larvae
Selective antifeedant activity Virulent Analysis of 10 mixture 2 of table to 3 instar larvae of diamondback moth
In table 10, mixture 2 handles for 24 hours, the AFC of 48h, 72h the food refusal Virulent Analysis of 3 instar larvae of diamondback moth50Respectively
0.0511g/L, 0.0283g/L, 0.0348g/L, wherein handle the food refusal AFC of 48h50Minimum, Antifeeding Effects are best.
Non-selective Antifeeding Effects of 5.3 mixtures 3 to diamondback moth larvae
5.3.1 Antifeeding Effects of the mixture 3 to diamondback moth larvae
Antifeeding Effects of 11 mixture 3 of table to 3 instar larvae of diamondback moth
Note:There is same letter to indicate under 95% level without significant difference (P=0.05) in table after data
As can be seen from Table 11, when maximum concentration 0.344g/L is handled, for 24 hours, the anti-food rate of 48h, 72h are respectively
90.02%, 90.13%, 91.51%, it is in raising trend.In low concentration 0.086g/L, 0.043g/L, 0.021g/L, anti-food rate
In 48h highests, 72h takes second place, minimum for 24 hours, is first to increase the trend dropped again.
5.3.2 selective antifeedant activity Virulent Analysis of the mixture 3 to diamondback moth larvae
Selective antifeedant activity Virulent Analysis of 12 mixture 3 of table to 3 instar larvae of diamondback moth
Table 12 is antifeedant activity Virulent Analysis of the mixture 3 to diamondback moth, for 24 hours, the AFC of 48h, 72h50Respectively 0.0957g/
L, 0.0551g/L, 0.0514g/L, on a declining curve, Antifeeding Effects enhance as processing time extends.
Non-selective Antifeeding Effects of 5.4 mixtures 4 to diamondback moth larvae
5.4.1 Antifeeding Effects of the mixture 4 to diamondback moth larvae
Antifeeding Effects of 13 mixture 4 of table to 3 instar larvae of diamondback moth
Note:There is same letter to indicate under 95% level without significant difference (P=0.05) in table after data
In maximum concentration 0.311g/L, 48h is handled, anti-food rate is up to 92.37%.In table 13, a total trend is, together
One concentration for the treatment of, processing time, the anti-food rate of 48h, 72h were minimum for 24 hours, and 48h highests, 72h is reduced again for 24 hours.When same processing
Between, anti-food rate is reduced as concentration for the treatment of reduces.
3.4.4.2 non selective antifeedant activity Virulent Analysis of the mixture 4 to diamondback moth larvae
Non selective antifeedant activity Virulent Analysis of 14 mixture 4 of table to diamondback moth larvae
Table 14 is non selective antifeedant activity Virulent Analysis of the mixture 4 to diamondback moth larvae, it can be seen that, is handled from table
Time for 24 hours, the AFC of 48h, 72h50Value is respectively 0.0893g/L, 0.0358g/L, 0.0447g/L.Wherein, processing time 48h
When AFC50It is worth minimum, Antifeeding Effects are best.
3.5 aalii saponin(es are compared with print chinaberry mixture is to the coefficient of synergism of diamondback moth larvae antifeedant activity
15 aalii saponin(e of table is compared with print chinaberry mixture is to the coefficient of synergism of 3 instar larvae antifeedant activity of diamondback moth
Note:A is aalii total saposins in table;B is print chinaberry active compound.Data are the calculated value of determination data for 24 hours in table.
After the test data of table 15 fully shows that aalii total saposins and print chinaberry active compound are compounded, synergistic effect is notable.
Claims (5)
1. a kind of using nimbin and aalii total saposins as the Pesticidal combination of active constituent, it is characterised in that nimbin is total with aalii
The weight ratio of saponin(e is 1:0.1~10.
2. as described in claim 1 using nimbin and aalii total saposins as the Pesticidal combination of active constituent, it is characterised in that
The weight ratio of nimbin and aalii total saposins is 1:6.38.
3. a kind of using nimbin and aalii total saposins as the usage of the Pesticidal combination of active constituent, it is characterised in that for agricultural
Pest control in production.
4. special as claimed in claim 3 using nimbin and aalii total saposins as the usage of the Pesticidal combination of active constituent
Sign is in agricultural production that insect pest is Lepidoptera.
5. special as claimed in claim 4 using nimbin and aalii total saposins as the usage of the Pesticidal combination of active constituent
Sign is in agricultural production that insect pest is diamondback moth (Plutella xylostella L.).
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CN111248211A (en) * | 2020-02-03 | 2020-06-09 | 扬州工业职业技术学院 | Azadirachtin and ginsenoside Rb1Mixture and use thereof |
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CN103113449A (en) * | 2013-03-11 | 2013-05-22 | 云南农业大学 | Aalii saponin compound as well as preparation method and application thereof |
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CN103113449A (en) * | 2013-03-11 | 2013-05-22 | 云南农业大学 | Aalii saponin compound as well as preparation method and application thereof |
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《植保员手册》编绘组,: "《植保员手册》", 31 May 2014, 上海科学技术出版社 * |
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CN111248211A (en) * | 2020-02-03 | 2020-06-09 | 扬州工业职业技术学院 | Azadirachtin and ginsenoside Rb1Mixture and use thereof |
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